| inhibitory effect of extracts of muscles of mackerel (scomber japonicus houttuyn) on hepatic glycogenolysis in rats. | the effects of extracts of muscles of mackerel (scomber japonicus; m-ext) on hepatic glycogenolysis were investigated by a rat liver perfusion method. m-ext inhibited glucagon- and cyclic adenosine monophosphate (amp)-induced glycogenolysis but was ineffective on phenylephrine-induced glycogenolysis. the contents of hepatic glycogen and cyclic amp, and phosphorylase and glycogen synthase activities in liver were measured after perfusion with glucagon. m-ext inhibited the increase of cyclic amp a ... | 1989 | 2743499 |
| study on hypoglycemic effects of muscles of pig (sus scrofa), chicken (gallus domesticus) and mackerel (scomber japonicus). | | 1988 | 3240483 |
| isolation and characterization of an asparagine-linked keratan sulfate from the skin of a marine teleost, scomber japonicus. | keratan sulfate was isolated from the skin of pacific mackerel (scomber japonicus) after exhaustive digestion with pronase followed by ethanol precipitation and fractionation on a cellulose column with 0.3% recovery of dried material. the keratan sulfate preparation was separated into four major fractions by dowex-1 column chromatography. the chemical and infrared spectrum analyses of the four fractions showed a high degree of heterogeneity in sulfation. since the carbohydrate-peptide linkage in ... | 1984 | 6230109 |
| sea bird mortality at cabo san lucas, mexico: evidence that toxic diatom blooms are spreading. | domoic acid was found to be responsible for an isolated event involving the massive death of brown pelicans (pelecanus occidentalis) in january 1996, at the tip of the baja california peninsula. the death of these sea birds was the result of feeding on mackerel (scomber japonicus) contaminated by domoic acid-producing diatoms (pseudonitzschia sp.). the number of dead birds (150 animals) found during a period of 5 days caused alarm and called for a governmental task force that would help to imple ... | 1997 | 9080599 |
| larvae and adults of hysterothylacium aduncum (rudolphi, 1802) (nematoda: anisakidae) in fishes and crustaceans in the south west atlantic. | hysterothylacium aduncum (rudolphi, 1802) is reported from five fishes and one invertebrate species. third-stage larvae were found in the crustacean themisto gaudichaudii and in mesenteries of the fishes engraulis anchoita and merluccius hubbsi; fourth-stage larvae were recovered from the digestive tract of m. hubbsi and scomber japonicus and adult specimens were obtained from the stomach and intestine of m. hubbsi, s. japonicus, genypterus blacodes and genypterus brasiliensis. nematodes are des ... | 1998 | 9754308 |
| total mercury in muscle of benthic and pelagic fish from the south adriatic sea (italy). | total mercury concentrations were measured from the muscle of different kinds of fish: yellow gurnard (trigla lucerna), red gurnard (aspitrigla cuculus) red fish (helicolenus dactylopterus), skate spp. (raje spp.), goldline (sarpa salpa), atlantic bonito (sarda sarda), mackerel (scomber scombrus), chub mackerel (scomber japonicus) caught in the south adriatic sea (south italy) in the period june/august 1995. the highest total mercury levels were found in the benthic marine organisms and particul ... | 1998 | 10366996 |
| heavy metal distribution in different fish species from the mauritania coast. | cadmium, copper, zinc and mercury concentrations were determined in pelagic and benthic fishes from the mauritania coast. the pelagic fishes consisted of the round sardinella sardinella aurita (clupeidae), the chub mackerel scomber japonicus (scombridae) and the atlantic horse mackerel trachurus trachurus (carangidae). four benthic species were considered, three serranidae: the painted comber serranus scriba, the golden grouper epinephelus costae, and the niger hind cephalopholis nigri and one m ... | 1999 | 10481293 |
| purification and cloning of an apoptosis-inducing protein derived from fish infected with anisakis simplex, a causative nematode of human anisakiasis. | while investigating the effect of marine products on cell growth, we found that visceral extracts of chub mackerel, an ocean fish, had a powerful and dose-dependent apoptosis-inducing effect on a variety of mammalian tumor cells. this activity was strikingly dependent on infection of the c. mackerel with the larval nematode, anisakis simplex. after purification of the protein responsible for the apoptosis-inducing activity, we cloned the corresponding gene and found it to be a flavoprotein. this ... | 2000 | 10903755 |
| histochemical and morphometric aspects of the lateral musculature of different species of teleost marine fish of the percomorphi order. | cross-sections of the lateral musculature of 19 species of teleost fish of the percomorphi order were studied histochemically and morphometrically. the red, pink and white muscles were observed in all the species except in seriola dumerilii and scomber japonicus, in which no pink muscle was found. the red fibres were the smallest due to their minimal diameters. histochemically and according to their location at least five types were found in the same species: typical red, in rosette, of the bord ... | 2000 | 11008368 |
| a comparison of myocardial beta-adrenoreceptor density and ligand binding affinity among selected teleost fishes. | this study quantified the cell surface beta-adrenoreceptor density and ligand binding affinity in the ventricular tissue of seven teleost species; skipjack tuna (katsowonus pelamis), yellowfin tuna (thunnus albacares), pacific mackerel (scomber japonicus), mahimahi (dolphin fish; coryphaena hippurus), sockeye salmon (oncorhynchus nerka), rainbow trout (oncorhynchus mykiss) and an antarctic nototheniid (trematomus bernacchii). beta-adrenoreceptor density varied by almost fourfold among these spec ... | 2000 | 11128445 |
| apoptosis-inducing protein, aip, from parasite-infected fish induces apoptosis in mammalian cells by two different molecular mechanisms. | aip (apoptosis-inducing protein) is a protein purified and cloned from chub mackerel infected with the larval nematode, anisakis simplex, which induces apoptosis in various mammalian cells including human tumor cell lines. aip has shown structural and functional homology to l-amino acid oxidase (lao) which oxidizes several l-amino acids including l-lysine and aip-induced apoptosis has been suggested to be mediated by h2o2 generated by lao activity of aip. in this study, we confirmed that recombi ... | 2001 | 11319613 |
| serum lipid peroxide and alpha-tocopherol concentrations and superoxide dismutase activity in captive bottle-nosed dolphins. | to evaluate serum lipid peroxide (lpo) and alpha-tocopherol concentrations and superoxide dismutase (sod) activity in captive bottle-nosed dolphins and to evaluate effects of storage on production of lpo in various marine fish. | 2001 | 11763187 |
| genetic markers in the study of anisakis typica (diesing, 1860): larval identification and genetic relationships with other species of anisakis dujardin, 1845 (nematoda: anisakidae). | genetic variation at 21 gene-enzyme systems was studied in a sample of an adult population of anisakis typica (diesing, 1860) recovered in the dolphin sotalia fluviatilis from the atlantic coast of brazil. the characteristic alleles, detected in this population, made it possible to identify as a. typica, anisakis larvae with a type i morphology (sensu berland, 1961) from various fishes: thunnus thynnus and auxis thazard from brazil waters, trachurus picturatus and scomber japonicus from madeiran ... | 2002 | 11912342 |
| quantification of the wake of rainbow trout (oncorhynchus mykiss) using three-dimensional stereoscopic digital particle image velocimetry. | although considerable progress has been made within the last decade in experimental hydrodynamic analyses of aquatic locomotion using two-dimensional digital particle image velocimetry (two-dimensional dpiv), data have been limited to simultaneous calculation of two out of the three flow velocity variables: downstream (u), vertical (v) and lateral (w). here, we present the first biological application of stereo-dpiv, an engineering technique that allows simultaneous calculation of u, v and w vel ... | 2002 | 12324537 |
| a cathepsin b-like enzyme from mackerel white muscle is a precursor of cathepsin b. | a cathepsin b-like enzyme from the white muscle of common mackerel scomber japonicus was a cysteine protease that hydrolyzed z-arg-arg-mca, the substrate for cathepsin b. in a partial purified cathepsin b-like enzyme preparation at 4 degrees c left over time, a converted enzyme that hydrolyzes z-arg-arg-mca and z-phe-arg-mca appeared in the preparation. the converted enzyme was purified from the cathepsin b-like enzyme, characterized and was identified as mackerel cathepsin b. these results sugg ... | 2002 | 12431398 |
| the occurrence and infection dynamics of anisakis larvae in the black-scabbard fish, aphanopus carbo, chub mackerel, scomber japonicus, and oceanic horse mackerel, trachurus picturatus from madeira, portugal. | larval stages of anisakis spp. (nematoda: anisakidae) were found encapsulated or free in the viscera and abdominal cavity of the black-scabbard fish, aphanopus carbo, chub mackerel, scomber japonicus and oceanic horse mackerel, trachurus picturatus in madeiran waters. the prevalence of infection reached 97.2% for a. carbo, 69.5% for s. japonicus and 62.5% for t. picturatus. considerable differences in parasite intensities between a. carbo and both s. japonicus and t. picturatus were found, with ... | 2003 | 12756070 |
| host and geographic range extensions of the north american strain of viral hemorrhagic septicemia virus. | viral hemorrhagic septicemia virus (vhsv) was isolated from populations of pacific sardine sardinops sagax from the coastal waters of vancouver island, british columbia, canada, and central and southern california, usa. the virus was also isolated from pacific mackerel scomber japonicus in southern california, from eulachon or smelt thaleichthys pacificus, and surf smelt hypomesus pretiosus pretiosus from oregon, usa. mortality and skin lesions typical of viral hemorrhagic septicemia in other ma ... | 2003 | 13677507 |
| [the parasite fauna of the chub mackerel (scombridae: scomber japonicus houttuyn, 1782) in the central-eastern atlantic (atlantic coast of the northern africa and the azores archipelago banks)]. | the parasite fauna of the chub mackerel scomber japonicus houtuym, 1782 was studied from the neritic areas of morocco, western sahara, mauritania and from the banks of the azores archipelago (the great meteor bank, the hyeres bank and the irving bank) in 1994-2001. twenty eight species of parasites of following group have been were found: coccidia (1 species), microsporidia (1), myxosporea (4), monogenea (4), cestoda (5), trematoda (5), acanthocephala (1) and nematoda (6). the differences betwee ... | 2004 | 15174392 |
| molecular analysis of the rebeccamycin l-amino acid oxidase from lechevalieria aerocolonigenes atcc 39243. | rebeccamycin, a member of the tryptophan-derived indolocarbazole family, is produced by lechevalieria aerocolonigenes atcc 39243. the biosynthetic pathway that specifies biosynthesis of this important metabolite is comprised of 11 genes spanning 18 kb of dna. a presumed early enzyme involved in elaboration of the rebeccamycin aglycone is encoded by rebo, located at the left-hand region of the reb gene cluster. the deduced protein product, rebo (51.9 kda), is an l-amino acid oxidase (l-aao) that ... | 2005 | 15743957 |
| effect of soy sauce on the antioxidative capacity of the gelatin gel food 'nikogori' measured using the chemiluminescence method. | oxygen-related free radicals have been suggested as a cause of aging and various diseases, for example, various cancers and rheumatoid arthritis. because of this a radical scavenger as an antioxidant has been sought in many foods. 'nikogori' gelatin gel from cooked fish is a traditional food in japan. recently, 'nikogori' has been noted as a readily consumed food for the elderly and those with medical problems associated with swallowing. in this study we report that the 'nikogori' gelatin gels m ... | 2005 | 16077950 |
| molecular characterization of larval anisakid nematodes from marine fishes of madeira by a pcr-based approach, with evidence for a new species. | one-hundred and fifteen anisakid larvae from 3 different fish hosts, aphanopus carbo, scomber japonicus, and trachurus picturatus, caught in madeiran waters, were identified by pcr-rflp. three distinct species were identified in a. carbo, namely anisakis simplex sensu srricto, anisakis pegreffii, and anisakis ziphidarum; 5 in s. japonicus, i.e., a. simplex s.s., a. pegreffii, anisakis physeteris, anisakis typica, and a. ziphidarum; and 3 in t. picturatus, i.e., a. simplex s.s., a. pegreffii, and ... | 2005 | 16539027 |
| substrate specificity of chitinases from two species of fish, greenling, hexagrammos otakii, and common mackerel, scomber japonicus, and the insect, tobacco hornworm, manduca sexta. | three chitinase isozymes, hochia, hochib, and hochic, were purified from the stomach of the greenling, hexagrammos otakii, by ammonium sulfate fractionation, followed by column chromatography on chitopearl basic bl-03 and cm-toyopearl 650s. the molecular masses and pis of hochia, hochib, and hochic are 62 kda and ph 5.7, 51 kda and ph 7.6, and 47 kda and ph 8.8, respectively. substrate specificities of these chitinases were compared with those of another fish stomach chitinase from the common ma ... | 2006 | 16636466 |
| molecular cloning, expression and phylogenetic analyses of parvalbumin in tilapia, oreochromis mossambicus. | the gene expression of parvalbumin (pvalb), a high-affinity calcium-binding protein and the major fish allergen, was significantly increased in the tilapia fry treated with methyltestosterone (mt) as examined using a subtractive hybridization assay. using the real-time quantitative pcr, we further confirmed the increased pvalb expression in the mt-treated tilapia fry. the 568 base pairs (bp) tilapia pvalb (tpvalb) cdna clone was fully sequenced and found to contain a coding region of 330 bp, whi ... | 2007 | 17094115 |
| [detection of fish dna in ruminant feed by pcr amplification]. | the japanese government has prohibited the use of seafood protein, as well as mammalian protein, in ruminant feed. there is an official method to detect meat and bone meal, but no method is yet available to detect fishmeal in ruminant feed. we tried to develop a suitable method to detect fishmeal in ruminant feed, similar to the official method "pcr detection of animal-derived dna in feed". our previously reported primers (fishcon5 and fishcon3-1) showed low sensitivity, so we designed new prime ... | 2006 | 17128872 |
| gill monogeneans of the chub mackerel, scomber japonicus from madeiran waters of the atlantic ocean, portugal. | five species of monogeneans were recovered from the gill filaments of 181 chub mackerel, scomber japonicus, from the madeiran waters of the atlantic ocean, portugal, during 2004/2005. the monogenean pseudokuhnia minor showed the highest prevalence (98.68%) and a mean intensity of 28.23, followed by kuhnia scombri (prevalence of 43.71% and mean intensity of 2.69) and k. scombercolias (prevalence of 39.1% and a mean intensity of 1.81). kuhnia sprostonae and grubea cochlear were rare, occurring in ... | 2007 | 17381864 |
| genetic and phenotypic comparison of nocardia seriolae isolated from fish in japan. | the phenotypic and genetic characterizations of 58 isolates of the fish pathogen nocardia seriolae, from amberjack, seriolae dumerili, yellowtail, seriola quinqueradiata, japanese flounder, paralichthys olivaceus, and chub mackerel, scomber japonicus, in japan from 1970-2005, were examined to investigate the epidemiological relationship between isolates. the phenotypic and genetic characterizations were determined by alpha-glucosidase activity and biased sinusoidal field gel electrophoresis (bsf ... | 2008 | 18482385 |
| morphological differences between larvae and in vitro-cultured adults of anisakis simplex (sensu stricto) and anisakis pegreffii (nematoda: anisakidae). | proper identification of anisakis species infecting host fishes is very important to both human health and fish disease diagnosis. the foremost problem in the identification of anisakis larvae in fishes is that l3 larvae cannot be easily differentiated morphologically, especially between a. simplex (sensu stricto) (s.s.) (rudolphi, 1809) and a. pegreffii campana-rouget et biocca, 1955. instead, molecular means such as allozyme, mitochondrial dna (mtdna) cox2 region and polymerase chain reaction- ... | 2008 | 18644463 |
| high-speed assessment of fat and water content distribution in fish fillets using online imaging spectroscopy. | a nondestructive method using online spectral imaging has been developed for quantitative measurements of moisture and fat distribution in six species of fish fillets: atlantic halibut (hippoglossus hippoglossus), catfish (icatalurus punctatus), cod (gadus morhua), mackerel (scomber japonicus), herring (clupea harengus), and saithe (pollachius virens). a spectral image cube was acquired for each fish fillet, and a subsampling approach for relating spectral and chemical features was applied. spec ... | 2008 | 18656933 |
| carcinogen adsorbent prepared from dna complex by gamma-ray irradiation. | the effects of gamma-ray irradiation on aqueous solutions of chub mackerel chromatin, salmon milt dna with cocl(2), mixtures of dna with type a gelatin, bovine serum albumin (bsa), cm-chitosan, carboxymethyl cellulose (cmc) and catinal (hydroxyethyl-cellulose, o-[2-hydroxy-3-(trimethyl ammonio)-propyl], chloride) and dna in the presence of polyfunctional monomers with the aim to insolubilize dna for preparing a novel carcinogen adsorbent have been studied. among those, precipitates or inhomogene ... | 2008 | 18727858 |
| anisakid parasites of fishes caught on the african shelf. | anisakids of scomber japonicus, trachyrincus scabrus, sardina pilchardus, merluccius polli, trachurus trachurus, and hoplostethus cadenati from african shelf are reported. parasites were extensively studied with respect to their morphology and sequence analysis of the rdna fragment (containing internal transcribed spacer [its]-1, 5.8s subunit and its-2); all of them are approximately 1,000 base pairs. digestion of the amplified region and its sequencing revealed the presence of a sibling species ... | 2009 | 19093709 |
| analysis of polycyclic aromatic hydrocarbons in fish: evaluation of a quick, easy, cheap, effective, rugged, and safe extraction method. | quechers method was evaluated for extraction of 16 pahs from fish samples. for a selective measurement of the compounds, extracts were analysed by lc with fluorescence detection. the overall analytical procedure was validated by systematic recovery experiments at three levels and by using the standard reference material srm 2977 (mussel tissue). the targeted contaminants, except naphthalene and acenaphthene, were successfully extracted from srm 2977 with recoveries ranging from 63.5-110.0% with ... | 2009 | 19750509 |
| effect of vacuum packaging and low-dose irradiation on the microbial, chemical and sensory characteristics of chub mackerel (scomber japonicus). | the effects of vacuum packaging followed by gamma irradiation treatment (1.5 kgy) on the shelf-life of fillets of chub mackerel (scomber japonicus) were examined, during chill storage. the control and the treated packs were analyzed periodically for chemical (tma, tbars, biogenic amines) and microbial characteristics. based on chemical and microbial data, vacuum packaging - by itself - was improper in extending the shelf-life of chub mackerel, estimated to 7 days. on the 7th day, tma and histami ... | 2009 | 19835766 |
| risk factors for human anisakis infection and association between the geographic origins of scomber japonicus and anisakid nematodes. | it has been reported that nearly all cases of anisakiasis in japan are caused by anisakis simplex sensu stricto. to elucidate this further, we investigated the presence of anisakis type i larvae and pseudoterranova decipiens in 218 scomber japonicus fish collected from the seas of japan. anisakis type i larvae were detected in 74.3% (162/218) of the fish, and 99.8% of the anisakis type i larvae comprised a. simplex sensu stricto and anisakis pegreffii. molecular identification techniques were us ... | 2010 | 19892425 |
| immunological characterization and distribution of three gnrh forms in the brain and pituitary gland of chub mackerel (scomber japonicus). | the presence of three gonadotropin-releasing hormone (gnrh) forms in the brain of the chub mackerel, scomber japonicus, namely, salmon gnrh (sgnrh), chicken gnrh-ii (cgnrh-ii), and seabream gnrh (sbgnrh), was confirmed by combined high performance liquid chromatography (hplc) and time-resolved fluoroimmunoassay (tr-fia). immunocytochemical localization of the three gnrh forms in the brain was investigated by using specific antisera, to elucidate possible roles of each gnrh form in reproduction i ... | 2009 | 19968470 |
| mercury, cadmium, lead and arsenic levels in three pelagic fish species from the atlantic ocean: intra- and inter-specific variability and human health risks for consumption. | three commonly consumed and commercially valuable fish species (sardine, chub and horse mackerel) were collected from the northeast and eastern central atlantic ocean in portuguese waters during one year. mercury, cadmium, lead and arsenic amounts were determined in muscles using graphite furnace and cold vapour atomic absorption spectrometry. maximum mean levels of mercury (0.1715 ± 0.0857 mg/kg, ww) and arsenic (1.139 ± 0.350 mg/kg, ww) were detected in horse mackerel. the higher mean amounts ... | 2010 | 21193008 |
| anisakis simplex larvae: infection status in marine fish and cephalopods purchased from the cooperative fish market in busan, korea. | the infection status of marine fish and cephalopods with anisakis simplex third stage larva (l3) was studied over a period of 1 year. a total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the cooperative fish market in busan, korea, from august 2006 to july 2007. they were examined for a. simplex l3 from the whole body cavity, viscera, and muscles. a. simplex l3 were confirmed by light microscopy. the overall infection rate reached 34 ... | 2011 | 21461267 |
| polycyclic aromatic hydrocarbon levels in three pelagic fish species from atlantic ocean: inter-specific and inter-season comparisons and assessment of potential public health risks. | the concentrations of 18 polycyclic aromatic hydrocarbons (pahs) were determined in three commercially valuable fish species (sardine, sardine pilchardus; chub mackerel, scomber japonicus; and horse mackerel, trachurus trachurus) from the atlantic ocean. specimens were collected seasonally during 2007-2009. only low molecular weight pahs were detected, namely, naphthalene, acenaphthene, fluorene and phenanthrene. chub mackerel (1.80-19.90μg/kgww) revealed to be significantly more contaminated th ... | 2011 | 22079181 |
| Anisakis simplex s.l. parasitization in mackerel (Scomber japonicus) caught in the North of Morocco - prevalence and analysis of risk factors. | Consumption of mackerel (Scomber japonicus) is very widespread in Morocco, where its production is an important economic pillar. We investigated the prevalence of infection by Anisakis spp. in mackerel caught in Moroccan waters, analyzing infection risk factors. The prevalence was generally higher in fish from the Atlantic (67.9%) than from the Mediterranean (57.0%), but they did not differ in the mean abundance, intensity, or prevalence of muscle parasitization. A. pegreffii was the predominant ... | 2011 | 21868119 |
| Bioaccumulation and Biomagnification of Total Mercury in Four Exploited Shark Species in the Baja California Peninsula, Mexico. | The present study determined the average mercury bioaccumulation in the muscle tissue of four shark species (Carcharhinus falciformis, Prionace glauca, Sphyrna zygaena and Isurus oxyrinchus) captured in the Baja California Peninsula. We also evaluated biomagnification of some prey consumed by sharks. All sharks' species had mercury levels over the limit specified by the Mexican government for human consumption. Blue shark (P. glauca) presented highest mercury values (1.96 ± 1.48 µg/g Hg d.w.) an ... | 2011 | 22187022 |
| effect of season on heavy metal contents and chemical compositions of chub mackerel (scomber japonicus) muscle. | seasonal variations of heavy metals concentrations and overall chemical compositions were determined in chub mackerel caught in the southern sea of korea. the average mercury and lead content varied between 0.04 and 0.08 mg/kg and between 0.01 and 0.02 mg/kg, respectively. seasonal variations were not detected in lead, but mercury displayed maximal values in winter (p < 0.05). a distinct seasonal pattern was found in crude fat content with maximal values in december and minimal values in april. ... | 2012 | 22251075 |
| change of volatile compounds in fresh fish meat during ice storage. | the change of volatile compounds in fresh fish meat during 3- to 4-d ice storage was investigated for several fishes using an electronic nose system and a gas chromatography-mass spectrometer (gc/ms) with headspace solid-phase micro-extraction (spme). principal component analyses for samples using the electronic nose system revealed that the increase of some volatile compounds during storage was rapid in sardine (sardinops melanostictus), jack mackerel (trachurus japonicus), and chub mackerel (s ... | 2011 | 22416694 |
| the pacific bluefin tuna (thunnus orientalis) dead end gene is suitable as a specific molecular marker of type a spermatogonia. | we developed a spermatogonial transplantation technique to produce donor-derived gametes in surrogate fish. our ultimate aim is to establish surrogate broodstock that can produce bluefin tuna. we previously determined that only type a spermatogonia (asg) could colonize recipient gonads in salmonids. therefore, it is necessary to develop a precise molecular marker that can distinguish asg in order to develop efficient spermatogonial transplantation methods. in this study, the pacific bluefin tuna ... | 2013 | 23913406 |
| analysis of bacterial community structure in saba-narezushi (narezushi of mackerel) by 16s rrna gene clone library. | narezushi, a derivation of sushi, is a traditional japanese food made by fermenting salted fish meat and cooked rice together. in this study, the microbial diversity of saba-narezushi (narezushi of mackerel, scomber japonicus) was analyzed by the 16s ribosomal rna gene clone library method. chemical composition was also analyzed to compare with different kinds of narezushi. the chemical composition of the narezushi was similar to those obtained from samma-narezushi. ninety-four clones were rando ... | 2011 | 24425983 |
| simultaneous detection and prevalence of allergens in anisakis species isolated from marine fishes. | this study was conducted to develop a multiplex reverse transcription (rt) pcr for the detection of anisakis allergens and to investigate the relationship between allergen profiles and anisakid larvae isolated from scomber japonicus, trichiurus lepturus, and conger myriaster in korea. the species of anisakis was determined using anisakis pegreffii-specific pcr and restriction fragment length polymorphism analysis. the prevalence and profiles of five ani s allergens were examined by multiplex rtp ... | 2016 | 27296426 |
| occurrence of anisakid nematode larvae in chub mackerel (scomber japonicus) caught off korea. | chub mackerel (scomber japonicus) is a pelagic fish species widely distributing in the indo-pacific and a commercially important fish species in korea. it is known to harbor anisakid nematodes larvae, and ingesting the raw or undercooked fish can accidentally cause human infection. in this study, we isolated the nematode larvae in 417 chub mackerel caught from 7 sampling locations around the korean peninsula in 2011 and 2012, and identified them by pcr-rflp of the its (internal transcribed space ... | 2014 | 25268324 |
| ecology and genetic structure of zoonotic anisakis spp. from adriatic commercial fish species. | consumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of live anisakis larvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. we investigated the population dynamics of anisakis spp. in commercially important fish-anchovies (anisakis), sardines (sardina pilchardus), european hake (merluccius merluccius), whiting (merlangius merlangus), chub mackerel (scomber japonicus), and atlantic bl ... | 2014 | 24317085 |
| molecular characterisation of anisakidae larvae from fish in adriatic sea. | in the present study, anisakids from: tuna (thunnus thynnus) fattened in the croatian farm in middle adriatic sea, three different feral fish species caught near tuna farm (trachurus trachurus, scomber japonicus and oblada melanura) and fish marketed in croatia (t. trachurus) were analysed by morphology and molecular methods. larvae were identified to the species level by pcr restriction fragment length polymorphism and characterised by sequencing of nuclear (internal transcribed spacer) and mit ... | 2012 | 22983167 |
| anisakis simplex sensu stricto and anisakis pegreffii: biological characteristics and pathogenetic potential in human anisakiasis. | anisakiasis is one of the most common fishborne helminthic diseases in japan, which is contracted by ingesting the larvae of the nematode anisakis spp. carried by marine fish. anisakis simplex sensu stricto (s.s.) and a. pegreffii are the dominant species in fish caught offshore japan. the present study aimed to identify the anisakid species infecting japanese patients and determine whether there is any difference in the pathogenetic potential of a. simplex (s.s.) and a. pegreffii. in total, 41 ... | 2012 | 22545961 |
| functional expression of l-lysine α-oxidase from scomber japonicus in escherichia coli for one-pot synthesis of l-pipecolic acid from dl-lysine. | l-pipecolic acid is a key component of biologically active molecules and a pharmaceutically important chiral building block. it can be stereoselectively produced from l-lysine by a two-step bioconversion involving l-lysine α-oxidase and ∆(1)-piperideine-2-carboxylae (pip2c) reductase. in this study, we focused on an l-lysine α-oxidase from scomber japonicus that was originally identified as an apoptosis-inducing protein (aip) and applied the enzyme to one-pot fermentation of l-pipecolic acid in ... | 2015 | 25547835 |
| identification and inhibition of histamine-forming bacteria in blue scad (decapterus maruadsi) and chub mackerel (scomber japonicus). | in this study, we investigated the differences in histamine accumulation between blue scad and chub mackerel and methods of inhibiting histamine-forming bacteria and controlling histamine accumulation in fish. the free histidine contents in blue scad and chub mackerel were 1.45 and 2.75 mg/g, respectively. the histamine-forming bacteria isolated from them were identified as citrobacter freundii, citrobacter braakii, and enterobacter aerogenes using 16s rdna sequence analysis, the vitek 2 compact ... | 2015 | 25710155 |
| helminth parasites of the chub mackerel scomber colias off the tunisian coast and their use in stock discrimination. | nine helminth parasites were used as biological tags to discriminate diverse areas of scomber colias gmelin, 1789. during three seasons, a total of 369 fish were examined in four zones off the tunisian coast, including bizerte in the north, kelibia and mahdia in the centre and zarzis in the south. discriminant analyses were used to identify distinct areas of s. colias. fish from bizerte were grouped as one area and were correlated negatively with the monogenean grubea cochlear and the digenean l ... | 2017 | 28215202 |
| parasites of the head of scomber colias (osteichthyes: scombridae) from the western mediterranean sea. | the metazoan parasite assemblage of the head of 30 specimens of the atlantic chub mackerel (scomber colias) from the western mediterranean sea was analysed. eight species of parasites were found, four mazocraeid monogeneans: grubea cochlear (prevalence = 10%), kuhnia scombercolias (59%), k. scombri (52%), pseudokuhnia minor (86%); three didymozoid trematodes: nematobothrium cf. faciale (21%), n. filiforme (41%), n. scombri (7%); and one laerneopodid copepod: clavelissa scombri (7%). results were ... | 2014 | 24570065 |
| comparative phylogeography of two monogenean species (mazocraeidae) on the host of chub mackerel, scomber japonicus, along the coast of china. | in the present paper, the phylogeographies of two monogenean species, pseudokuhnia minor and kuhnia scombri, on the same species of host, scomber japonicus, were studied. fragments of the mitochondrial cytochrome c oxidase subunit 1 gene were sequenced for 264 individuals of p. minor and 224 individuals of k. scombri collected from 10 localities along the coast of china. genetic diversity of k. scombri was higher than that of p. minor, which may imply that p. minor has a lower evolution rate and ... | 2016 | 26888047 |
| ultrastructure of the spermatozoon of the digenean lecithocladium excisum (rudolphi, 1819) (hemiuroidea: hemiuridae), a parasite of marine teleosts in senegal. | the present study describes the ultrastructure of the mature spermatozoon of lecithocladium excisum (rudolphi, 1819) (digenea: hemiuroidea: hemiuridae) from the stomach of the marine teleost scomber japonicus houttuyn (scombridae) captured in the atlantic ocean, off dakar (senegal). the ultrastructural organization of the spermatozoon of l. excisum follows the general model described in most digeneans. it presents two axonemes of the 9+'1' pattern of the trepaxonemata, nucleus, mitochondrion and ... | 2012 | 23136797 |
| cestodes of the blue shark, prionace glauca (linnaeus 1758), (carcharhiniformes: carcharhinidae), off the west coast of baja california sur, mexico. | the cestode species recovered from the spiral intestines of 27 blue sharks (prionace glauca) (linnaeus, 1758) are reported from the western coast of baja california sur (bcs). the sampling was undertaken on a monthly basis from january 2003 to january 2004. the helminthological examination indicated the presence of four species of cestodes: platybothrium auriculatum yamaguti, 1952; prosobothrium japonicum yamaguti, 1934; anthobothrium caseyi (yamaguti, 1934) ruhnke & caira, 2009; and paraorygmat ... | 2016 | 27394312 |
| molecular characterization of muscle-parasitizing didymozoid from a chub mackerel, scomber japonicus. | didymozoids found in the muscles of marine fish are almost always damaged because they are usually found after being sliced. therefore, identifying muscle-parasitizing didymozoids is difficult because of the difficulty in collecting non-damaged worms and observing their organs as key points for morphological identification. moreover, muscle-parasitizing didymozoids are not easily found because they parasitize at the trunk muscles. therefore, muscle-parasitizing didymozoid classification has not ... | 2015 | 26204013 |
| dolichol biosynthesis: the occurrence of epoxy dolichol in skipjack tuna liver. | polyisoprenoid alcohols from the livers of temperate sea fish (skipjack tuna, chub mackerel, red sea bream and rainbow trout) were analyzed by using 2d-tlc, electrospray ionization (esi) mass spectrometry and nmr methods. dolichols (dols) were detected in all the fish livers, and they were composed of 19-22 isoprene units with dol-20 as the predominant prenolog. in addition, dol-like family compounds were found by using 2d-tlc on skipjack tuna samples. these compounds were found to have a larger ... | 2014 | 24866245 |
| the effects of extraction methods on the contents of fatty acids, especially epa and dha in marine lipids. | this study aims to evaluate the efficiency of trans-methylation methods in fish oil obtained using soxhlet extraction method and bligh and dyer method and also to observe the effects of extraction methods on the contents of polyunsaturated fatty acids [pufas, especially eicosapentaenoic acid (epa) and docosahexaenoic acid (dha)] in five marine species: sardine (sardinella aurita), anchovy (engraulis encrasicolus), sea bream (sparus aurata), brushtooth lizardfish (saurida undosquamis) and chub ma ... | 2012 | 22010951 |
| enzymatic properties of fish muscle aldolase. | 1. aldolases were isolated from the ordinary muscle of red sea bream pagrus major, pacific mackerel scomber japonicus, and carp cyprinus carpio by ammonium sulfate fractionation, followed by ion-exchange chromatography on deae-cellulose and cm-sepharose cl-6b columns, and examined for enzymatic properties. 2. the aldolases showed the highest activity in a ph range from 6.8-7.8 km values for fructose-1,6-bisphosphate ranged from 0.025-0.10 mm. 3. irrespective of fish species, aldolase activity wa ... | 1989 | 2924547 |
| enzymatic properties of glyceraldehyde-3-phosphate dehydrogenase from fish muscle. | 1. glyceraldehyde-3-phosphate dehydrogenase was isolated from the ordinary muscle of red sea bream pagrus major, pacific mackerel scomber japonicus and carp cyprinus carpio by ammonium sulfate fractionation, followed by deae-sepharose cl-6b and deae-cellulose column chromatography and sephadex g-150 gel filtration, and examined for enzymatic properties. 2. their optimum ph values in the backward reaction ranged from 7.8 to 8.2, and km values from 1.56 to 1.90 mm. 3. irrespective of the species o ... | 1989 | 2776431 |
| thermal modulation of the toxicokinetics of benzo[a]pyrene in isolated hepatocytes of sablefish (anoplopoma fimbria), black rockfish (sebastes melanops), and chub mackerel (scomber japonicus). | hepatocytes from sablefish (anoplopoma fimbria), black rockfish (sebastes melanops) and chub mackerel (scomber japonicus) were isolated from 11 degrees c acclimated animals. the uptake, metabolism, and excretion of benzo[a]pyrene (b[a]p) in hepatocytes was measured at 6, 11 and 19 degrees c. chub mackerel hepatocyte uptake rates were significantly lower (0.012 +/- 0.003 microg/s per g cell) at 11 degrees c than black rockfish (0.028 +/- 0.009 microg/s per g cell) or sablefish (0.032 +/- 0.012 mi ... | 1999 | 10622431 |
| histamine levels in fish from markets in lima, perú. | illnesses associated with seafood are an important public health concern worldwide, particularly considering the steady increase in seafood consumption. however, research about the risks associated with seafood products is scarce in developing countries. histamine fish poisoning is the most common form of fish intoxication caused by seafood and usually presents as an allergic reaction. this condition occurs when fish are not kept appropriately refrigerated and histamine is formed in the tissues. ... | 2009 | 19517744 |
| why does gila elegans have a bony tail? a study of swimming morphology convergence. | caudal-fin-based swimming is the primary form of locomotion in most fishes. as a result, many species have developed specializations to enhance performance during steady swimming. specializations that enable high swimming speeds to be maintained for long periods of time include: a streamlined body, high-aspect-ratio (winglike) caudal fin, a shallow caudal peduncle, and high proportions of slow-twitch ("red") axial muscle. we described the locomotor specializations of a fish species native to the ... | 2016 | 27157474 |
| parasites as biological tags for stock discrimination in marine fish from south american atlantic waters. | the use of parasites as biological tags in population studies of marine fish in the south-western atlantic has proved to be a successful tool for discriminating stocks for all species to which it has been applied, namely: scomber japonicus, engraulis anchoita, merluccius hubbsi and cynoscion guatucupa, the latter studied on a broader geographic scale, including samples from uruguayan and brazilian waters. the distribution patterns of marine parasites are determined mainly by temperature-salinity ... | 2007 | 17578590 |
| patterns of distribution and spatial indicators of ecosystem change based on key species in the southern benguela. | several commercially and ecologically important species in the southern benguela have undergone southward and eastward shifts in their distributions over previous decades, most notably the small pelagic fish sardine sardinops sagax and anchovy engraulis encrasicolus. understanding these changes and their implications is essential in implementing an ecosystem approach to fisheries in the southern benguela and attempting to appreciate the potential impacts of future environmental change. to invest ... | 2016 | 27441550 |
| effect of freezing/thawing process in different sizes of blue fish in the mediterranean through lysosomal enzymatic tests. | the assessment of freshness of different sizes of blue fish (engraulis encrasicolus 12 cm, sardina pilchardus 15 cm, trachurus trachurus 40 cm, scomber japonicus colias 60 cm) was carried out using non-conventional enzymatic methods. the activities of the three lysosomal enzymes (α-glucosidase (ag), β-galactosidase (b-gal) and β-n-acetylglucosamidase (b-na)) in extracts of blue fish muscle were measured over a period of 21 days of storage. a significant increase (p<0.05) of ag activity was obser ... | 2014 | 24262525 |
| maximum sustainable speed, energetics and swimming kinematics of a tropical carangid fish, the green jack caranx caballus. | maximum sustained swimming speeds, swimming energetics and swimming kinematics were measured in the green jack caranx caballus (teleostei: carangidae) using a 41 l temperature-controlled, brett-type swimming-tunnel respirometer. in individual c. caballus [mean ±s.d. of 22·1 ± 2·2 cm fork length (l(f) ), 190 ± 61 g, n = 11] at 27·2 ± 0·7° c, mean critical speed (u(crit)) was 102·5 ± 13·7 cm s⁻¹ or 4·6 ± 0·9 l(f) s⁻¹. the maximum speed that was maintained for a 30 min period while swimming steadil ... | 2012 | 22650430 |
| swimming performance studies on the eastern pacific bonito sarda chiliensis, a close relative of the tunas (family scombridae) ii. kinematics. | the swimming kinematics of the eastern pacific bonito sarda chiliensis at a range of sustained speeds were analyzed to test the hypothesis that the bonito's swimming mode differs from the thunniform locomotor mode of tunas. eight bonito (fork length fl 47.5+/-2.1 cm, mass 1.25+/-0.15 kg) (mean +/- s.d.) swam at speeds of 50-130 cm s(-1) at 18+/-2 degrees c in the same temperature-controlled water tunnel that was used in previous studies of tunas. kinematics variables, quantified from 60 hz video ... | 2003 | 12847120 |
| swimming kinematics of juvenile kawakawa tuna (euthynnus affinis) and chub mackerel (scomber japonicus). | the swimming kinematics of two active pelagic fishes from the family scombridae were compared to test the hypothesis that the kawakawa tuna (euthynnus affinis) uses the thunniform mode of locomotion, in which the body is held more rigid and undergoes less lateral movement in comparison with the chub mackerel (scomber japonicus), which uses the carangiform swimming mode. this study, the first quantitative kinematic comparison of size-matched scombrids, confirmed significantly different swimming k ... | 2000 | 11003821 |
| maximum sustainable speeds and cost of swimming in juvenile kawakawa tuna (euthynnus affinis) and chub mackerel (scomber japonicus). | tunas (scombridae) have been assumed to be among the fastest and most efficient swimmers because they elevate the temperature of the slow-twitch, aerobic locomotor muscle above the ambient water temperature (endothermy) and because of their streamlined body shape and use of the thunniform locomotor mode. the purpose of this study was to test the hypothesis that juvenile tunas swim both faster and more efficiently than their ectothermic relatives. the maximum sustainable swimming speed (u(max), t ... | 2000 | 11003820 |
| effects of chicken breast meat on quality properties of mackerel (scomber japonicus) sausage. | this study was performed to evaluate the effects of chicken breast meat on the quality of mackerel sausages. the mackerel sausages were manufactured by additions of 5%, 7%, and 10% of chicken breast meat. the lightness of mackerel sausages showed no significant differences between the control and addition groups. the redness increased in a dose-dependent manner, but the yellowness decreased significantly with the addition of 7% chicken breast meat (p<0.05). the whiteness value of mackerel sausag ... | 2014 | 26760754 |
| characterization of the ribosomal rna gene of kudoa neothunni (myxosporea: multivalvulida) in tunas (thunnus spp.) and kudoa scomberi n. sp. in a chub mackerel (scomber japonicus). | kudoa neothunni is the first described kudoa species having six shell valves and polar capsules, previously assigned to the genus hexacapsula arai and matsumoto, 1953. since its genetic analyses remain to be conducted, the present study characterizes the ribosomal rna gene (rdna) using two isolates from a yellowfin tuna (thunnus albacares) with post-harvest myoliquefaction and a northern bluefin tuna (thunnus thynnus) without tissue degradation. spores of the two isolates localized in the myofib ... | 2013 | 23455941 |