| [considerations on the antibiotic activity (m.i.c.) revealed in 247 serotypes of salmonella]. | 247 strains of salmonella, isolated in brescia province duriing 1973-1974 have been typed by serological methods. the mic has been determined, using the following antibiotics: chloramphenicol, tetracycline hcl, ampicillin, doxycycline, rifampicin, cephazolin, carbenicillin, nifuratel, gentamicin, aminosidine, trimetho-prim-sulphamethoazole, nalidixic acid. the pattern of resistence of the various serotypes is quite constant. the relationship between diffusion and epidermiological factors are dis ... | 1975 | 1045970 |
| olive (olea europea) and privet (ligustrum vulgare) pollen allergens. identification and cross-reactivity with grass pollen proteins. | protein blotting studies showed that three olive pollen components with mol. wts approximately 18-19, 20 and 40 kd can be considered to be major allergens. for privet pollen, the highest recognition frequencies were for allergens of mol. wts approximately 20, approximately 19, approximately 40 and approximately 70 kd. when results with the 62 subjects examined were separated into groups corresponding to their geographical locations, viz. italy, france and australia, subjects sensitized to olive, ... | 1992 | 1528191 |
| [integration of plasmid rp1 with the chromosome of escherichia coli k-12 reca. 2 classes of hfr strains]. | integration of broad host range rp1 plasmid into the chromosome of escherichia coli k-12 reca- cells has been studied. using temperature-sensitive for replication plasmids pvd1 and pvd3, the derivatives of rp1, it has been shown that integration of rp1 into the bacterial chromosome results in formation of two classes of hfr strains. properties of these hfrs have been examined. from the data obtained, it has been concluded that the plasmid integration and formation of one of the hfrs classes appe ... | 1983 | 6317516 |
| inspiratory on-switch evoked by mesencephalic stimulation: activity of medullary respiratory neurones. | the activity of medullary inspiratory and expiratory neurones was studied in urethan-chloralose anaesthetized cats during stimulus-evoked inspiratory phase (inspiratory on-switch). all neurones were characterized according to their axonal destination (i.e. bulbospinal neurons or vagal motoneurones) or the absence of such axonal projections (i.e. propriobulbar neurones), and to their location in the dorsal or ventral respiratory nuclei. the inspiratory on-switch effects were elicited during expir ... | 1984 | 6499974 |
| inspiratory on-switch evoked by stimulation of mesencephalic structures: a patterned response. | the effects of brief tetanic electrical stimulation (50 to 100 ms) of the mesencephalic central gray matter and reticular formation on the inspiratory "on-switch" mechanism were studied during expiration (e phase) in cats anaesthetized with urethan-chloralose. stimulation during the e phase evoked powerful effects on the phrenic nerve discharge comprising (1) a primary response (prim. r.) during the train; (2) a patterned response (patt. r.) resembling that of the normal inspiratory (i) phase an ... | 1983 | 6617795 |
| [poisoning by the fruit of the privet (ligustrum vulgare)]. | | 1983 | 6636246 |
| inspiratory on-switch evoked by stimulation of the mesencephalon: activity of phrenic and laryngeal motoneurones. | in anaesthetized cats (chloralose-urethan) the effects of brief tetanic electrical stimulation (50 to 100 ms) of the mesencephalic central gray matter and reticular formation on the inspiratory on-switch were studied during the expiratory (e) phase on the gross and unitary activities of phrenic, laryngeal inspiratory and laryngeal expiratory nerves. on the inspiratory laryngeal and phrenic nerves, stimulation elicited a short latency gross response concomitant with the train: the inspiratory pri ... | 1984 | 6745360 |
| evaluation of serum specific ige and skin responsiveness to allergenic extracts of oleaceae pollens (olea europaea, fraxinus excelsior and ligustrum vulgare) "in patients with respiratory allergy. | in the last few years an increasing interest for olea europaea (o.e.) pollen has been developed in many countries. several authors have studied the aerobiological, allergenic, epidemiological and clinical features of this pollinosis. the aim of our study was to evaluate cutaenous and serological responses to other oleaceae pollens allergens (ligustrum vulgare (l.v.) and fraxinus excelsior (f.e.) in patients with skin positivity to olea europaea pollen extract. twenty-three atopic rhinitis and/or ... | 1995 | 7631595 |
| absence of circadian rhythm in the disposition of melatonin in the cow. | five adult pasture-bred prim holstein cows were used to document the time invariance of melatonin disposition in the cow. melatonin was administered by an intravenous route as a bolus (5 micrograms/kg of body weight) at either 1430 or 2230. data were analysed according to a three-compartment open model with melatonin elimination from the central compartment. the mean (+/- sd) kinetic parameters describing melatonin disposition, during day and night, respectively, were plasma clearances: 0.0267 + ... | 1993 | 8106960 |
| simultaneous determination of felbamate, primidone, phenobarbital, carbamazepine, two carbamazepine metabolites, phenytoin, and one phenytoin metabolite in human plasma by high-performance liquid chromatography. | an isocratic liquid-chromatographic method employing one extraction step has been developed for the quantitation of five drugs and three metabolites in human plasma. the method uses 0.100-ml aliquots of human plasma and two internal standards. chromatographic conditions include a 4.6 mm x 150 mm spherisorb ods2, 3 microns a high-performance liquid chromatography, (hplc) column, a phosphate buffer-acetonitrile-methanol (700:160:140) mobile phase, and ultraviolet (uv) absorbance detection at 210 n ... | 1994 | 8160262 |
| the main allergen of olea europaea (ole e i) is also present in other species of the oleaceae family. | three major pollen allergens from fraxinus excelsior, ligustrum vulgare and syringa vulgaris belonging to the oleaceae family were purified. monoclonal antibodies previously raised against the main allergen of olea europaea (ole e i) were used for their purification by affinity chromatography. the three new purified allergens were able to bind human ige from serum of olive-allergic patients in a way analogous to ole e i. crossed radioimmunoelectrophoresis of the four allergens, using anti-olive ... | 1993 | 8319129 |
| evolving t cell responses in murine schistosomiasis. th2 cells mediate secondary granulomatous hypersensitivity and are regulated by cd8+ t cells in vivo. | the dynamics of t cell maturation and regulation were examined during the granulomatous response to schistosoma mansoni eggs under conditions of primary (prim), secondary vigorous (vig), and secondary immunomodulated (mod) immunity. the patterns of vig and mod gr (gr) formation were established in naive cba mice by transfer of lymphoid cells from s. mansoni-infected donors at the vig and mod stages of infection. sequential production of ifn, il-2, and il-4 was assessed at the gr and in draining ... | 1993 | 8335935 |
| anticonvulsant-induced changes in tissue manganese, zinc, copper, and iron concentrations in wistar rats. | human epileptics have been reported to have low blood manganese (mn) concentrations in comparison to nonepileptics, an observation that is important because mn deficiency can increase seizure susceptibility in experimental animals. factors that have been suggested to contribute to the low blood mn levels in epileptics include anticonvulsant use, seizure-induced tissue redistribution of mn, and genetics; in the present study, the first of these possibilities was tested. wistar rats were fed semip ... | 1993 | 8345801 |
| activator effect of coinjected enhancers on the muscle-specific expression of promoters in zebrafish embryos. | the transient expression of reporter gene constructs in embryos provides a powerful tool to characterise cis-acting transcriptional elements of the genes involved in development. in the present study, we have analysed the expression pattern of several muscle-specific and ubiquitous regulatory sequences in microinjected zebrafish embryos. by using a fast and reproducible coinjection strategy, the mosaic expression of lacz reporter gene was monitored in wholemount embryos injected with sequences c ... | 1997 | 9211424 |
| cross-reactivity of olea europaea with other oleaceae species in allergic rhinitis and bronchial asthma. | cross-reactivity between pollen extracts of four species of oleaceae was studied: olive (olea europaea), ash (fraxinus excelsior), privet (ligustrum vulgare), and lilac (syringa vulgaris). thus, 51 patients and 13 atopic controls were studied, by means of intracutaneous skin tests, histamine-release tests against the four extracts, and specific ige to o. europaea. the proteic content of the four extracts was assessed by sds-page and immunoblotting, and similarity of all the extracts studied was ... | 1997 | 9284982 |
| the replication protein a binding site in simian virus 40 (sv40) t antigen and its role in the initial steps of sv40 dna replication. | physical interactions of simian virus 40 (sv40) large tumor (t) antigen with cellular dna polymerase alpha-primase (pol/prim) and replication protein a (rpa) appear to be responsible for multiple functional interactions among these proteins that are required for initiation of viral dna replication at the origin, as well as during lagging-strand synthesis. in this study, we mapped an rpa binding site in t antigen (residues 164 to 249) that is embedded within the dna binding domain of t antigen. t ... | 1998 | 9811712 |
| proteic composition of corpora amylacea in the bovine mammary gland. | histochemical studies of mammary gland sections obtained from healthy lactating prim' holstein cows contained numerous corpora amylacea, mainly located in active alveoli. observations by electron microscopy revealed a cauliflower shape, with a fibrillar or multilayered organization. mineral studies confirmed the presence of high calcium levels (12.3% of dry matter) and phosphorus (7.4%) in the corpora amylacea composition. these bodies stained positive to von kossa silver nitrate and to periodic ... | 1998 | 9839482 |
| surface plasmon resonance measurements reveal stable complex formation between p53 and dna polymerase alpha. | surface plasmon resonance measurements were used for detecting and quantifying protein-protein interactions between the tumor suppressor protein p53, the sv40 large t antigen (t-ag), the cellular dna polymerase alpha-primase complex (pol-prim), and the cellular single-strand dna binding protein rpa. highly purified p53 protein bound to immobilized t-ag with an apparent binding constant of 2 x 10(8) m(-1). binding of p53 to rpa was in the same order of magnitude with a binding constant of 4 x 10( ... | 1999 | 9989827 |
| [functional treatment of acute achilles tendon rupture. a histological, immunohistological and ultrasonographic analysis of the animal model]. | in 105 rabbits the course of healing was examined at 1, 2, 4, 8 and 12 weeks (21 rabbits per group) after an experimental achilles tendon rupture. the following treatment modalities were compared: a. operative functional treatment (resorbable suture, kleinert technique) b. operative functional treatment with fibrin glue c. primary functional treatment. for the functional (after)-treatment a special orthosis was applied. a 7.5 mhz ultrasound probe was used for the ultrasonographic evaluation. the ... | 1999 | 10420825 |
| changes in immunomodulatory activity of human mononuclear cells after cultivation with leaf decoctions from the genus ligustrum l. | leaf decoctions from ligustrum vulgare (lv) and ligustrum delavayanum (ld) were studied for candidacidal activity, phagocytic activity studied on human mononuclear cells (mo) and complement activated by the classical pathway. candidacidal activity was studied on candida albicans sc 1539 incubated with mo. the decoction of ld increased the candidacidal activity of mo, whereas lv did not show any effect. the phagocytic activity of mo was decreased by the decoction of lv, whereas ld did not change ... | 1999 | 10594942 |
| postpartum subestrus in dairy cows: comparison of treatment with prostaglandin f2 alpha or gnrh + prostaglandin f2 alpha + gnrh. | two experiments (experiment 1, 185 cows in 1996/97; experiment 2, 168 cows in 1997/98) were conducted with prim holstein dairy cattle in the mayenne region of france to investigate subestrus. cows which had not been observed in estrus since calving were allocated alternately to treatment groups between 60 and 90 d post partum as follows: experiment 1-group 1: gnrh (day 0, 100 micrograms i.m.), pgf2 alpha (day 7, 25 mg i.m.), gnrh (day 9, 100 micrograms i.m.) and ai (day 10); group 2: pgf2 alpha ... | 1999 | 10735129 |
| protein-protein interactions of the primase subunits p58 and p48 with simian virus 40 t antigen are required for efficient primer synthesis in a cell-free system. | dna polymerase alpha-primase (pol-prim, consisting of p180-p68-p58-p48), and primase p58-p48 (prim(2)) synthesize short rna primers on single-stranded dna. in the sv40 dna replication system, only pol-prim is able to start leading strand dna replication that needs unwinding of double-stranded (ds) dna prior to primer synthesis. at high concentrations, pol-prim and prim(2) indistinguishably reduce the unwinding of dsdna by sv40 t antigen (tag). rna primer synthesis on ssdna in the presence of rep ... | 2000 | 10747950 |
| crystal structure of a dna-dependent rna polymerase (dna primase). | primases are essential components of the dna replication apparatus in every organism. they catalyze the synthesis of oligoribonucleotides on single-stranded dna, which subsequently serve as primers for the replicative dna polymerases. in contrast to bacterial primases, the archaeal enzymes are closely related to their eukaryotic counterparts. we have solved the crystal structure of the catalytic primase subunit from the hyperthermophilic archaeon pyrococcus furiosus at 2.3 a resolution by multiw ... | 2001 | 11135672 |
| different regions of primase subunit p48 control mouse polyomavirus and simian virus 40 dna replication in vitro. | dna polymerase alpha-primase (pol-prim), a complex consisting of four subunits, is the major species-specific factor for mouse polyomavirus (pyv) and simian virus 40 (sv40) dna replication. although p48 is the most conserved subunit of pol-prim, it is required for in vitro pyv dna replication but can inhibit cell-free sv40 dna replication. production of chimeric human-mouse p48 revealed that different regions of p48 are involved in supporting pyv dna replication and inhibiting sv40 dna replicati ... | 2001 | 11160673 |
| preprocessing implementation for microarray (prim): an efficient method for processing cdna microarray data. | cdna microarray technology is useful for systematically analyzing the expression profiles of thousands of genes at once. although many useful results inferred by using this technology and a hierarchical clustering method for statistical analysis have been confirmed using other methods, there are still questions about the reproducibility of the data. we have therefore developed a data processing method that very efficiently extracts reproducible data from the result of duplicate experiments. it i ... | 2001 | 11160997 |
| cytotoxic effects of plant extracts from the families fabaceae, oleaceae, philadelphaceae, rosaceae and staphyleaceae. | a human transformed line of hela cells was treated with extracts prepared from gymnocladus dioicus, holodiscus discolor, stephanandra tanakae, ligustrum delavayanum, ligustrum vulgare and staphylea pinnata. the study evaluating the influence of the active extracts on hela cell division also reveals their acute, delayed and combined effect. a 35% degradation of hela cells was found after 72 h treatment with 62.5 microg/ml of the extract isolated from stephanandra tanakae. a 100% lysis of hela cel ... | 2001 | 11180517 |
| two immunologically distinct human dna polymerase alpha-primase subpopulations are involved in cellular dna replication. | metabolic labeling of primate cells revealed the existence of phosphorylated and hypophosphorylated dna polymerase alpha-primase (pol-prim) populations that are distinguishable by monoclonal antibodies. cell cycle studies showed that the hypophosphorylated form was found in a complex with pp2a and cyclin e-cdk2 in g1, whereas the phosphorylated enzyme was associated with a cyclin a kinase in s and g2. modification of pol-prim by pp2a and cdks regulated the interaction with the simian virus 40 or ... | 2001 | 11259605 |
| multiple phosphorylation sites of dna polymerase alpha-primase cooperate to regulate the initiation of dna replication in vitro. | dna polymerase alpha-primase (pol-prim) is the only enzyme that can start dna replication de novo. the 180-kda (p180) and 68-kda (p68) subunits of the human four-subunit enzyme are phosphorylated by cyclin-dependent kinases (cdks) in a cell cycle-dependent manner. cyclin a-cdk2 physically interacts with pol-prim and phosphorylates n-terminal amino acids of the p180 and the p68 subunits, leading to an inhibition of pol-prim in initiating cell-free sv40 dna replication. mutation of conserved putat ... | 2001 | 11502743 |
| amino acids 257 to 288 of mouse p48 control the cooperation of polyomavirus large t antigen, replication protein a, and dna polymerase alpha-primase to synthesize dna in vitro. | although p48 is the most conserved subunit of mammalian dna polymerase alpha-primase (pol-prim), the polypeptide is the major species-specific factor for mouse polyomavirus (pyv) dna replication. human and murine p48 contain two regions (a and b) that show significantly lower homology than the rest of the protein. chimerical human-murine p48 was prepared and coexpressed with three wild-type subunits of pol-prim, and four subunit protein complexes were purified. all enzyme complexes synthesized d ... | 2001 | 11507202 |
| mutational analysis of simian virus 40 t-antigen primosome activities in viral dna replication. | the recruitment of dna polymerase alpha-primase (pol-prim) is a crucial step in the establishment of a functional replication complex in eukaryotic cells, but the mechanism of pol-prim loading and the composition of the eukaryotic primosome are poorly understood. in the model system for simian virus 40 (sv40) dna replication in vitro, synthesis of rna primers at the origin of replication requires only the viral tumor (t) antigen, replication protein a (rpa), pol-prim, and topoisomerase i. on rpa ... | 2002 | 11967327 |
| role of the p68 subunit of human dna polymerase alpha-primase in simian virus 40 dna replication. | dna polymerase alpha-primase (pol-prim) is a heterotetramer with dna polymerase and primase activities. the polymerase (p180) and primase (p48 and p58) subunits synthesize primers and extend them, but the function of the remaining subunit (p68) is poorly understood. genetic studies in yeast suggested an essential role for the p68 ortholog in early s phase prior to the hydroxyurea-sensitive step, possibly a regulatory role in initiation of dna replication, but found no evidence for an essential f ... | 2002 | 12138179 |
| effects of enteric bacterial and cyanobacterial lipopolysaccharides, and of microcystin-lr, on glutathione s-transferase activities in zebra fish (danio rerio). | cyanobacteria (blue-green algae) can produce a variety of toxins including hepatotoxins e.g. microcystins, and endotoxins such as lipopolysaccharides (lps). the combined effects of such toxins on fish are little known. this study examines the activities of microsomal (m) and soluble (s) glutathione s-transferases (gst) from embryos of the zebra fish, danio rerio at the prim six embryo stage, which had been exposed since fertilisation to lps from different sources. a further aim was to see how ac ... | 2002 | 12200087 |
| [a pharmacodynamic research on chromone glucosides of fangfeng]. | to investigate the pharmacological activity of prim-o-glucosylcimifugin and 4'-o-beta-d-glucosyl-5-o-methylvisamminol con tained in fangfeng. | 2000 | 12512455 |
| purification and characterization of an inverting stereo- and enantioselective sec-alkylsulfatase from the gram-positive bacterium rhodococcus ruber dsm 44541. | whole cells of rhodococcus ruber dsm 44541 were found to hydrolyze (+/-)-2-octyl sulfate in a stereo- and enantiospecific fashion. when growing on a complex medium, the cells produced two sec-alkylsulfatases and (at least) one prim-alkylsulfatase in the absence of an inducer, such as a sec-alkyl sulfate or a sec-alcohol. from the crude cell-free lysate, two proteins responsible for sulfate ester hydrolysis (designated rs1 and rs2) were separated from each other based on their different hydrophob ... | 2003 | 12732552 |
| [physician founders of pediatric pulmonology in serbia]. | initial steps in the field of pediatric pulmology in serbia were made by the first serbian pediatricians dr. platon papakostopulo (1864-1915) and dr. milenko materni (1875-1929). later on these steps were continued by prim dr. ljubomir vulović (1896-1970). until the discovery of anti-tuberculotics, lung tuberculosis was one of the most frequent and most serious diseases among the adults and the children's population. prof. dr. smilja kostić-joksić (1895-1981) and prof. dr borivoje tasovac (1907- ... | 2003 | 14692139 |
| structure of a bifunctional dna primase-polymerase. | genome replication generally requires primases, which synthesize an initial oligonucleotide primer, and dna polymerases, which elongate the primer. primase and dna polymerase activities are combined, however, in newly identified replicases from archaeal plasmids, such as prn1 from sulfolobus islandicus. here we present a structure-function analysis of the prn1 primase-polymerase (prim-pol) domain. the crystal structure shows a central depression lined by conserved residues. mutations on one side ... | 2004 | 14730355 |
| assembly of the replication initiation complex on sv40 origin dna. | the assembly of the complex that forms over the simian virus 40 origin to initiate dna replication is not well understood. this complex is composed of the virus-coded t antigen and three cellular proteins, replication protein a (rpa), dna polymerase alpha/primase (pol/prim) and topoisomerase i (topo i) in association with the origin. the order in which these various proteins bind to the dna was investigated by performing binding assays using biotinylated origin dna. we demonstrate that in the pr ... | 2004 | 14960720 |
| the replication protein of the sulfolobus islandicus plasmid prn1. | the thermoacidophile crenarchaeote sulfolobus ssp. is one of the best-studied archaea. cryptic and conjugative plasmids as well as viruses have been described for this genus. for the majority of the genetic elements only the genome sequence and the basic characteristics were determined. in contrast the fusellovirus ssv1 and the cryptic plasmid prn1, which is the smallest known genetic element of the crenarchaeota, have been studied in more detail. the three gene products of the plasmid prn1 have ... | 2004 | 15046580 |
| anti-helicobacter pylori activities of six iranian plants. | helicobacter pylori is the major worldwide cause of bacterial gastrointestinal infections in adults and children. antibiotic therapy and a combination of two or three drugs have been widely used to eradicate these infections. however, development of drug resistance in bacteria calls for new sources of drugs, and plants seem to be a logical source of new antibacterial compounds. | 2004 | 15068416 |
| genomic, cdna, and embryonic expression analysis of zebrafish transforming growth factor beta 3 (tgfbeta3). | tgfbeta3, a member of the transforming growth factor beta family, regulates a spectrum of biological processes and is involved in mammalian pulmonary and craniofacial development. homologs of human tgfbeta3 have been identified in several vertebrate species. we sequenced a cdna clone of zebrafish tgfbeta3, consisting of a 271-bp 5' untranslated region, a 1,233-bp open reading frame that encodes a predicted 410 amino acid peptide, and a 527-bp 3' untranslated region. using 5' rapid amplification ... | 2005 | 15739231 |
| motherhood mitigates aging-related decrements in learning and memory and positively affects brain aging in the rat. | the current work examined spatial learning and memory (i.e., latencies to find a baited food well) in age-matched nulliparous, primiparous and multiparous (null, prim and mult, zero, one or two pregnancies and lactations, respectively). we tested at 6, 12, 18 and 24 months of age in a dry land version of the morris water maze (main task), and at 12, 18 and 24 months in the same task in which the original location of the baited well was changed (reversal task). we show that prim/mult rats, compar ... | 2005 | 15982524 |
| reduction of body-weight gain enhances in vitro embryo production in overfed superovulated dairy heifers. | the aim of our study was to test whether a reduction in dietary intake could improve in vitro embryo production in superovulated overfed dairy heifers. cumulus-oocyte complexes of 16 prim' holstein heifers (14 +/- 1 months old) were collected by ovum pick-up (opu), every 2 weeks following superovulation treatment with 250 microg fsh, before being matured and fertilized in vitro. embryos were cultured in synthetic oviduct fluid medium for 7 days. heifers were fed with hay, soybean meal, barley, m ... | 2006 | 16595729 |
| searching for interpretable rules for disease mutations: a simulated annealing bump hunting strategy. | understanding how amino acid substitutions affect protein functions is critical for the study of proteins and their implications in diseases. although methods have been developed for predicting potential effects of amino acid substitutions using sequence, three-dimensional structural, and evolutionary properties of proteins, the applications are limited by the complication of the features and the availability of protein structural information. another limitation is that the prediction results ar ... | 2006 | 16984653 |
| [simultaneous analysis of six effective components in the anti-alzheimer's disease effective component group of xiao-xu-ming decoction]. | a method based on high performance liquid chromatography (hplc) was developed for the quantitative determination of six components in an anti-alzheimer medicine, xiao-xu-ming decoction, which is an effective prescription in treating stroke and the sequela of stroke by herbalist doctors for thousands years. the effective component group (ecg) was made according to the results of high-throughput screening, and the curative effect of ecg was validated on aging rats. in this method an ods column was ... | 2007 | 17432581 |
| cloning and expression of translation elongation factor 2 (ef-2) in zebrafish. | we have identified a developmentally regulated gene translation elongation factor 2 (ef-2) in zebrafish (genbank accession no. aaq91234). analysis of dna sequence of zebrafish ef-2 shows that the 2826 bp cdna spans an open reading frame from nucleotide 55 to 2631 and encodes a protein of 858 amino acids. it shares an identity of 92, 93, 93, 92, 79 and 80% in amino acid sequence to human, mouse, chinese hamster, gallus gullus, c. elegans and drosophila ef-2, respectively. zebrafish ef-2 protein h ... | 2008 | 18300156 |
| interactions of water stress and solar irradiance on the physiology and biochemistry of ligustrum vulgare. | we studied the interactive effects of water stress and solar irradiance on physiological and biochemical traits in ligustrum vulgare l., with special emphasis on antioxidant enzymes and flavonoids. water relations, photosynthetic performance, plant growth, activities of antioxidant enzymes and of phenylalanine-ammonia-lyase, and concentrations of nonstructural carbohydrates and phenylpropanoids were measured in plants growing in 12% (shade) or 100% (sun) sunlight and supplied with 100 or 40% of ... | 2008 | 18381268 |
| a sensitive liquid chromatography-mass spectrometry method for simultaneous determination of two active chromones from saposhnikovia root in rat plasma and urine. | a sensitive and efficient liquid chromatography-mass spectrometry method was developed and validated for the simultaneous determination of two active chromones (prim-o-glucosylcimifugin and 4'-o-d-glucosyl-5-o-methylvisamminol) from saposhnikovia root in rat plasma and urine. the plasma or urine samples were prepared by protein precipitation. chromatographic separation of the two active chromones from matrix interferences was achieved on an angilent tc-c(18) column with a mobile phase consisted ... | 2008 | 18485839 |
| phenotype-specific cpg island methylation events in a murine model of prostate cancer. | aberrant dna methylation plays a significant role in nearly all human cancers and may contribute to disease progression to advanced phenotypes. study of advanced prostate cancer phenotypes in the human disease is hampered by limited availability of tissues. we therefore took advantage of the transgenic adenocarcinoma of mouse prostate (tramp) model to study whether three different phenotypes of tramp tumors (prim, late-stage primary tumors; aip, androgen-independent primary tumors; and met, meta ... | 2008 | 18519676 |
| complete genome sequence of a virulent isolate of arabis mosaic virus from privet (ligustrum vulgare). | | 2008 | 18563287 |
| the binding of topoisomerase i to t antigen enhances the synthesis of rna-dna primers during simian virus 40 dna replication. | topoisomerase i (topo i) is required for the proper initiation of simian virus 40 (sv40) dna replication. this enzyme binds to sv40 large t antigen at two places, close to the n-terminal end and near the c-terminal end of the helicase domain. we have recently demonstrated that the binding of topo i to the c-terminal site is necessary for the stimulation of dna synthesis by topo i and for the formation of normal amounts of completed daughter molecules. in this study, we investigated the mechanism ... | 2008 | 18702506 |
| dual inhibition of metallopeptidases ace and nep by extracts, and iridoids from ligustrum vulgare l. | in order to confirm the traditional use of ligustrum vulgare l. (common privet, oleaceae) we investigated the inhibitory activity of different extracts from leaves (lle), flowers (lfle) and fruits (lfre) on metallopeptidases ace and nep. | 2008 | 18790039 |
| [effect of prim-o-glucosylcimifugin and 4'-o-beta-d-glucosyl-5-o-methylvisamminol con on proliferation of smooth muscle cell stimulated by tnf-alpha]. | to investigate the effect of prim-o-glucosylcimifugin and 4'-o-p-d-glucosyl-5-o-methylvisa-mminol con on the proliferation of smooth muscle cell stimulated by tnf-alpha. | 2008 | 19066065 |
| a novel sequence variation in the transactivation regulating domain of the human androgen receptor. | to study a novel sequence variation within the androgen receptors' n-terminal cag repeat region and possible resulting consequences for the receptors' three-dimensional (3d) protein structure. | 2009 | 19361789 |
| prim-snping: a primer designer for cost-effective snp genotyping. | many kinds of primer design (pd) software tools have been developed, but most of them lack a single nucleotide polymorphism (snp) genotyping service. here, we introduce the web-based freeware "prim-snping," which, in addition to general pd, provides three kinds of primer design functions for cost-effective snp genotyping: natural pd, mutagenic pd, and confronting two-pair primers (ctpp) pd. the natural pd and mutagenic pd provide primers and restriction enzyme mining for polymerase chain reactio ... | 2009 | 19480636 |
| [effect of chuanxiong fangfeng baizhi prescription on isolated rabbit basilar artery]. | to observe the effect of the main constituents and its combination from chuanxiong fangfeng baizhi prescription on isolated rabbit basilar artery. | 2009 | 19771875 |
| the sv40 large t-antigen origin binding domain directly participates in dna unwinding. | the origin binding domain (obd) of sv40 large t-ag serves a critical role during initiation of dna replication to position t-ag on the origin. after origin recognition, t-ag forms a double hexamer over the origin. within each hexamer, the obd adopts a lock washer structure where the origin recognizing a1 and b2 loops face toward the helicase domain and likely become unavailable for binding dna. in this study, we investigated the role of the central channel of the obd hexamer in dna replication b ... | 2010 | 20108984 |
| host-specific replication of bk virus dna in mouse cell extracts is independently controlled by dna polymerase alpha-primase and inhibitory activities. | the activation of the human polyomavirus bk causes polyomavirus-associated nephropathy in immunocompromised humans. studies of the virus have been restricted since the virus dna replication is species specific. cell-based and cell-free dna replication systems, including the bk virus (bkv) monopolymerase dna replication system using purified proteins, reproduce the species specificity (28). therefore, the major host proteins comprising this assay, dna polymerase alpha-primase (pol-prim) and repli ... | 2010 | 20392840 |
| lack of association between hypoxia inducible factor-1 alpha gene polymorphisms and biopsy-proven giant cell arteritis. | since the transcription factor hypoxia-inducible factor 1 (hif-1) is a key early mediator of the response to ischemia and giant cell arteritis (gca) is a polygenic disease leading to severe ischemic complications, in the present study we analysed for first time the implication of two hif-1alpha gene polymorphisms in the susceptibility to and clinical expression of gca. | 2010 | 20412701 |
| a specific docking site for dna polymerase {alpha}-primase on the sv40 helicase is required for viral primosome activity, but helicase activity is dispensable. | replication of simian virus 40 (sv40) dna, a model for eukaryotic chromosomal replication, can be reconstituted in vitro using the viral helicase (large tumor antigen, or tag) and purified human proteins. tag interacts physically with two cellular proteins, replication protein a and dna polymerase α-primase (pol-prim), constituting the viral primosome. like the well characterized primosomes of phages t7 and t4, this trio of proteins coordinates parental dna unwinding with primer synthesis to ini ... | 2010 | 20685648 |
| plasma and milk kinetics of eprinomectin following topical or oral administration to lactating chinese holstein cows. | chinese holstein, bred by mating the holstein-friesian to chinese yellow cattle, is a major dairy cattle breed in china. eprinomectin is widely used in the treatment of nematode and ectoparasite infections in lactating cattle. the pharmacokinetics of eprinomectin in the plasma and milk were determined in chinese holstein cows following topical (at 0.5 mg kg(-1)) or oral (at 0.2 mg kg(-1)) administration. for topical administration, the concentrations of eprinomectin in plasma reached peak values ... | 2010 | 20851527 |
| isolated perfused lung extraction and hplc-esi-ms(n) analysis for predicting bioactive components of saposhnikoviae radix. | a novel strategy for predicting bioactive components in traditional chinese material herb was proposed, using isolated perfused rat lung (ipl) extraction and high performance liquid chromatography\tandem mass spectrometry (hplc-ms(n)) analysis. the hypothesis is that when the ipl is perfused with the extract of saposhnikoviae radix (esr), the potential bioactive components in the esr should selectively combine with the receptor or channel of lung, by changing the ph of perfused liquid, the combi ... | 2010 | 20970279 |
| estimation of genetic parameters and effects of cytoplasmic line on scrotal circumference and semen quality traits in angus bulls. | the purpose of this study was to estimate the heritability of scrotal circumference (sc) and semen traits, genetic correlations between sc and semen quality traits, and the effect of cytoplasmic line on sc and semen traits. breeding soundness exam (bse) data were collected on registered angus bulls at 4 ranches over 7 yr. the american angus association provided historical pedigree information to estimate the effect of cytoplasmic line on sc and semen quality traits. after editing, the evaluated ... | 2010 | 21036934 |
| intestinal permeability of the constituents from the roots of saposhnikovia divaricata in the human caco-2 cell monolayer model. | the bidirectional intestinal permeability of the active constituents from the roots of saposhnikovia divaricata, including four coumarins, anomalin ( 1), 5-methoxy-7-(3,3-dimethylallyloxy)coumarin ( 2), decursin ( 3), and decursinol angelate ( 4), as well as four chromones, cimifugin ( 5), prim- o-glucosylcimifugin ( 6), 3'- o-angeloylhamaudol ( 7), and sec- o-glucosylhamaudol ( 8), was studied by using the caco-2 cell monolayer. these compounds were assayed by hplc, and their transport paramete ... | 2011 | 21308612 |
| large-scale analysis of acute ethanol exposure in zebrafish development: a critical time window and resilience. | in humans, ethanol exposure during pregnancy causes a spectrum of developmental defects (fetal alcohol syndrome or fas). individuals vary in phenotypic expression. zebrafish embryos develop fas-like features after ethanol exposure. in this study, we ask whether stage-specific effects of ethanol can be identified in the zebrafish, and if so, whether they allow the pinpointing of sensitive developmental mechanisms. we have therefore conducted the first large-scale (>1500 embryos) analysis of acute ... | 2011 | 21625530 |
| hplc determination of antilipoxygenase activity of a water infusion of ligustrum vulgare l. leaves and some of its constituents. | the aim of the study was a hplc evaluation of the lipoxygenase activity inhibiting activity of a water infusion of ligustrum vulgare l. leaves and selected isolates from it. the antiradical activity of the water infusion was determined using dpph, abts and frap tests. oleuropein and echinacoside concentrations in the water infusion were determined by hplc. water infusion, echinacoside and oleuropein were used for an antilipoxygenase activity assay using lipoxygenase isolated from rat lung cytoso ... | 2011 | 21959294 |
| primidone metabolism in renal insufficiency and acute intoxication. | primidone (prim) is metabolized into phenobarbital (pb) and phenylethylmalonamide (pema). during anticonvulsant therapy with prim under normal conditions pb represents by fat the largest portion of the total concentration of all three components (prim + pb + pema). in combined therapy with diphenylhydantoin (dph), and during chronic prim overdosage, the relative concentration of pb is even higher. a case of renal insufficiency while on prim therapy and a case of acute prim intoxication are prese ... | 2001 | 92538 |
| effects of antiepileptic drugs on concentration of serum proteins and immunoglobulins of epileptic patients. | serum of normal volunteers and serum of epileptic patients receiving either a single drug or combined antiepileptic therapy with phenytoin (phen); carbamazepine (cbz); primidone (prim); phenobarbital (phb) were examined and showed: no differences with regard to total proteins, albumins and a/g ratio. the alpha 1-globulin fraction increased to 4.4% with phen/cbz (p less than 0.001) and decreased to 1.7% with phen/prim = (p less than 0.001). the alpha 2-globulins corresponded to 7.2% (p less than ... | 2014 | 3949148 |
| a new family of polymerases related to superfamily a dna polymerases and t7-like dna-dependent rna polymerases. | using sequence profile methods and structural comparisons we characterize a previously unknown family of nucleic acid polymerases in a group of mobile elements from genomes of diverse bacteria, an algal plastid and certain dna viruses, including the recently reported sputnik virus. using contextual information from domain architectures and gene-neighborhoods we present evidence that they are likely to possess both primase and dna polymerase activity, comparable to the previously reported prim-po ... | 2008 | 18834537 |
| microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities. | this review gives an overview on the occurrence of sulfatases in prokaryota, eukaryota and archaea. the mechanism of enzymes acting with retention or inversion of configuration during sulfate ester hydrolysis is discussed taking two complementary examples. methods for the discovery of novel alkyl sulfatases are described by way of sequence-based search and enzyme induction. a comprehensive list of organisms with their respective substrate scope regarding prim- and sec-alkyl sulfate esters allows ... | 2013 | 24352732 |
| asymmetric preparation of prim-, sec-, and tert-amines employing selected biocatalysts. | this account focuses on the application of ω-transaminases, lyases, and oxidases for the preparation of amines considering mainly work from our own lab. examples are given to access α-chiral primary amines from the corresponding ketones as well as terminal amines from primary alcohols via a two-step biocascade. 2,6-disubstituted piperidines, as examples for secondary amines, are prepared by biocatalytical regioselective asymmetric monoamination of designated diketones followed by spontaneous rin ... | 2013 | 23794796 |
| the substrate tolerance of alcohol oxidases. | alcohols are a rich source of compounds from renewable sources, but they have to be activated in order to allow the modification of their carbon backbone. the latter can be achieved via oxidation to the corresponding aldehydes or ketones. as an alternative to (thermodynamically disfavoured) nicotinamide-dependent alcohol dehydrogenases, alcohol oxidases make use of molecular oxygen but their application is under-represented in synthetic biotransformations. in this review, the mechanism of copper ... | 2015 | 26153139 |
| cultural adaptation and psychometric testing of the short form of iranian childbirth self efficacy inventory. | to assess maternal confidence in her ability to cope with labor, a measure of childbirth self efficacy is necessary. | 2013 | 24719682 |
| antinociceptive effects of prim-o-glucosylcimifugin in inflammatory nociception via reducing spinal cox-2. | we measured anti-nociceptive activity of prim-o-glucosylcimifugin (pog), a molecule from saposhnikovia divaricate (turcz) schischk. anti-nociceptive or anti-inflammatory effects of pog on a formalin-induced tonic nociceptive response and a complete freund's adjuvant (cfa) inoculation-induced rat arthritis pain model were studied. single subcutaneous injections of pog produced potent anti-nociception in both models that was comparable to indomethacin analgesia. anti-nociceptive activity of pog wa ... | 2016 | 27257008 |
| non-invasive imaging of disseminated candidiasis in zebrafish larvae. | disseminated candidiasis caused by the pathogen candida albicans is a clinically important problem in hospitalized individuals and is associated with a 30 to 40% attributable mortality(6). systemic candidiasis is normally controlled by innate immunity, and individuals with genetic defects in innate immune cell components such as phagocyte nadph oxidase are more susceptible to candidemia(7-9). very little is known about the dynamics of c. albicans interaction with innate immune cells in vivo. ext ... | 2012 | 22872032 |
| suitable in vitro culture of eimeria bovis meront ii stages in bovine colonic epithelial cells and parasite-induced upregulation of cxcl10 and gm-csf gene transcription. | we here established a suitable in vitro cell culture system based on bovine colonic epithelial cells (bcec) for the development of eimeria bovis merozoites i and the characterization of early parasite-induced innate epithelial host cell reactions as gene transcription of proinflammatory molecules. both primary and permanent bcec (bcec (rim) and bcec(perm)) were suitable for e. bovis merozoite i invasion and subsequent development of meronts ii leading to the release of viable merozoites ii. e. b ... | 2015 | 25982572 |
| a response regulator promotes francisella tularensis intramacrophage growth by repressing an anti-virulence factor. | the orphan response regulator pmra is essential for the intramacrophage growth and survival of francisella tularensis. pmra was thought to promote intramacrophage growth by binding directly to promoters on the francisella pathogenicity island (fpi) and positively regulating the expression of fpi genes, which encode a type vi secretion system required for intramacrophage growth. using both chip-seq and rna-seq we identify those regions of the f. tularensis chromosome occupied by pmra and those ge ... | 2016 | 27169554 |
| discovering hotspots in functional genomic data superposed on 3d chromatin configuration reconstructions. | the spatial organization of the genome influences cellular function, notably gene regulation. recent studies have assessed the three-dimensional (3d) co-localization of functional annotations (e.g. centromeres, long terminal repeats) using 3d genome reconstructions from hi-c (genome-wide chromosome conformation capture) data; however, corresponding assessments for continuous functional genomic data (e.g. chromatin immunoprecipitation-sequencing (chip-seq) peak height) are lacking. here, we demon ... | 2016 | 26869583 |
| crystal structure of the human pol α b subunit in complex with the c-terminal domain of the catalytic subunit. | in eukaryotic dna replication, short rna-dna hybrid primers synthesized by primase-dna polymerase α (prim-pol α) are needed to start dna replication by the replicative dna polymerases, pol δ and pol ϵ. the c terminus of the pol α catalytic subunit (p180c) in complex with the b subunit (p70) regulates the rna priming and dna polymerizing activities of prim-pol α. it tethers pol α and primase, facilitating rna primer handover from primase to pol α. to understand these regulatory mechanisms and to ... | 2015 | 25847248 |
| insights into eukaryotic primer synthesis from structures of the p48 subunit of human dna primase. | dna replication in all organisms requires polymerases to synthesize copies of the genome. dna polymerases are unable to function on a bare template and require a primer. primases are crucial rna polymerases that perform the initial de novo synthesis, generating the first 8-10 nucleotides of the primer. although structures of archaeal and bacterial primases have provided insights into general priming mechanisms, these proteins are not well conserved with heterodimeric (p48/p58) primases in eukary ... | 2014 | 24239947 |
| the detection and quantitation of protein oligomerization. | there are many different techniques available to biologists and biochemists that can be used to detect and characterize the self-association of proteins. each technique has strengths and weaknesses and it is often useful to combine several approaches to maximize the former and minimize the latter. here we review a range of methodologies that identify protein self-association and/or allow the stoichiometry and affinity of the interaction to be determined, placing an emphasis on what type of infor ... | 2012 | 22949109 |
| mannitol synthesis in higher plants : evidence for the role and characterization of a nadph-dependent mannose 6-phosphate reductase. | mannitol is a major photosynthetic product in many algae and higher plants. photosynthetic pulse and pulse-chase (14)c-radiolabeling studies with the mannitol-synthesizing species, celery (apium graveolens l.) and privet (ligustrum vulgare l.), showed that mannose 6-phosphate (m6p) and mannitol 1-phosphate were among the early photosynthetic products. a nadph-dependent m6p reductase was detected in these species (representing two different higher plant families), and the enzyme was purified to a ... | 1992 | 16668806 |
| methyl tert butyl ether targets developing vasculature in zebrafish (danio rerio) embryos. | disruption of vascular endothelial growth factor (vegf) signaling during early development results in abnormal angiogenesis and increased vascular lesions. embryonic exposure to 0.625-10mm methyl tert butyl ether (mtbe), a highly water soluble gasoline additive, resulted in a dose dependent increase in pooled blood in the common cardinal vein (ccv), cranial hemorrhages and abnormal intersegmental vessels (isvs). the ec50s for the lesions ranked in terms of likelihood to occur with mtbe exposure ... | 2011 | 21684239 |
| pharmacokinetic interaction of astragaloside iv with atractylenolide i and prim-o-glucosylcimifugin in male sprague dawley rats. | astragaloside iv, atractylenolide i, and prim-o-glucosylcimifugin are main medicinal components of the traditional chinese medicine prescription yu-ping-feng which is composed of three herbs: astragalus membranaceus, atractylodes macrocephala, and saposhnikovia divaricata. this study is aimed to assess the influence of atractylenolide i and prim-o-glucosylcimifugin on the pharmacokinetic profile of astragaloside iv so as to investigate the pharmacokinetic mechanisms of the yu-ping-feng prescript ... | 2014 | 24452462 |
| differences during the first lactation between cows cloned by somatic cell nuclear transfer and noncloned cows. | lactation performance is dependent on both the genetic characteristics and the environmental conditions surrounding lactating cows. however, individual variations can still be observed within a given breed under similar environmental conditions. the role of the environment between birth and lactation could be better appreciated in cloned cows, which are presumed to be genetically identical, but differences in lactation performance between cloned and noncloned cows first need to be clearly evalua ... | 2016 | 27016834 |
| metabolic and oxidative status of saanen goats of different parity during the peripartum period. | the aim of this study was to research changes in metabolic and antioxidative status of saanen goats of different parity occurring during the peripartum period. blood samples were taken on 10-7 and 3-1 d prepartally and 1-3, 14 and 28 d postpartally from goats allocated in three groups according to their parity: primiparous (prim), goats that kidded the 2nd or 3rd time (mid), and goats that kidded 4 or more times (multi)). metabolic profile parameters (non-esterified fatty acids (nefa), β-hydroxy ... | 2015 | 26511664 |
| seasonal changes between one- and two-phasic response of plant leaves to heat stress. | plant leaves normally respond to increasing injurious temperatures with a more or less continuous increase of tissue damage. a discontinuous response comprising a first lethal or sublethal temperature range which is followed by a non-injurious temperature range and a second, the lethal temperature range appeared to be an exception. the data with populus deltoides x simonii show that a two-phasic response is most pronounced after heat shocks of 15 s and was no longer detected after exposure to he ... | 1977 | 28309148 |
| fifteen-year phenological plant species and meteorological trends in central italy. | the present study was carried out in a phenological garden in central italy that contains vegetative clones of shrubs and trees common to several international phenological gardens, such as cornus sanguinea l.; corylus avellana l.; ligustrum vulgare l.; robinia pseudoacacia l.; salix acutifolia willd. and sambucus nigra l. vegetative plant growth monitoring was carried out weekly using common international keys: bbch07, bud break and leaf unfolding; bbch19, young unfolded leaf; bbch91, adult lea ... | 2014 | 23435513 |
| the role of cctpt1 in scale and early embryo development in common carp (cyprinus carpio, cyprinidae). | the full length cdna sequence of the tpt1/tctp (tumor protein, translationally-controlled1) gene was identified from common carp (cyprinus carpio, cyprinidae), and was designated as cctpt1 gene. the cds is 510 bp and encodes a 170-amino acid peptide with a typical tpt1 signature 2 domain, and is a typical tpt1 protein. the deduced amino acid sequence of tpt1 shared significant identity with the tpt1 from other animals. a phylogenetic tree analysis revealed that the common carp tpt1 protein has t ... | 2013 | 24122620 |
| myosin heavy chain expression in cranial, pectoral fin, and tail muscle regions of zebrafish embryos. | to investigate whether different myosin heavy chain (mhc) isoforms may constitute myofibrils in the trunk and tail musculature and if their respective expression may be regulated by spadetail (spt) and no tail (brachyury), we identified and characterized mrna expression patterns of an embryonic- and tail muscle-specific mhc gene (named myhz2) during zebrafish development in wild type, spt, and ntl mutant embryos. the identified myhz2 mhc gene encodes a polypeptide containing 1,935 amino acids. d ... | 2002 | 12412043 |
| regulation of ahr signaling by nrf2 during development: effects of nrf2a deficiency on pcb126 embryotoxicity in zebrafish (danio rerio). | the embryotoxicity of co-planar pcbs is regulated by the aryl hydrocarbon receptor (ahr), and has been reported to involve oxidative stress. ahr participates in crosstalk with another transcription factor, nfe2l2, or nrf2. nrf2 binds to antioxidant response elements to regulate the adaptive response to oxidative stress. to explore aspects of the crosstalk between nrf2 and ahr and its impact on development, we used zebrafish (danio rerio) with a mutated dna binding domain in nrf2a (nrf2a(fh318/fh ... | 2015 | 26325326 |
| factors affecting chilled storage of zebrafish (danio rerio) embryos. | chilled storage of zebrafish embryos was investigated at a temperature that arrests embryonic development as this technique might offer interesting practical applications. five parameters played an important role for chilled storage: (a) storage temperature, (b) development stage of embryos, (c) storage solution (extender), (d) postchilling treatment, and (e) inhibition of growth of microorganisms by antibiotics. the optimal chilling temperature was 8 degrees c. prim-5 stage (24h postfertilizati ... | 2009 | 19406462 |
| effects of methanol and developmental arrest on chilling injury in zebrafish (danio rerio) embryos. | stage-dependent chilling sensitivity has been reported for many species of fish embryos. most of these studies reveal that developmental stages beyond 50% epiboly are less sensitive to chilling, but the chilling sensitivity accelerates rapidly at subzero temperatures. in this study, the effects of methanol and developmental arrest on chilling injury were studied using zebrafish (danio rerio) embryos at 64-cell, 50% epiboly, 6-somite, prim-6 and long-bud stages. embryos were exposed to methanol o ... | 2003 | 12559459 |
| differential scanning calorimetry studies of intraembryonic freezing and cryoprotectant penetration in zebrafish (danio rerio) embryos. | nucleation temperatures of intraembryonic water and cryoprotectant penetration in zebrafish embryos were studied using differential scanning calorimetry. the effects of embryo developmental stage, dechorionation, partial removal of yolk, cooling rate, and cryoprotectant treatment on the temperatures of intraembryonic freezing were investigated. embryo stages were found to have a significant effect on the nucleation temperatures of intact embryos. freeze onset temperatures of -11.9 +/- 1.5, -15.6 ... | 2001 | 11479909 |
| effect of cooling rate and partial removal of yolk on the chilling injury in zebrafish (danio rerio) embryos. | high chilling sensitivity is one of the main obstacles to successful cryopreservation of zebrafish embryos. so far the nature of the chilling injury in fish embryos has not been clear. the aim of this study is to investigate the effect of cooling rate and partial removal of yolk on chilling injury in zebrafish embryos. zebrafish embryos at 64-cell, 50%-epiboly, 6-somite and prim-6 stages were cooled to either 0 degrees c or -5 degrees c at three different cooling rates: slow (0.3 degrees c/min o ... | 2001 | 11393222 |
| characterization of a zebrafish (danio rerio) desmin cdna: an early molecular marker of myogenesis. | desmin is a muscle-specific protein and a constitutive subunit of the intermediate filaments (if) in skeletal, cardiac and smooth muscles. it is an early marker of skeletal muscle myogenesis. we have characterized a clone of desmin cdna from an embryonic zebrafish (danio rerio) cdna library. the full-length cdna comprised 1798 nucleotides, encoding a protein of 473 amino acids. the predicted amino acid sequence of the zebrafish desmin shares a high degree of similarity to other vertebrate desmin ... | 2000 | 10929203 |
| uptake, toxicity, and effects on detoxication enzymes of atrazine and trifluoroacetate in embryos of zebrafish. | the uptake, toxicity, and elimination of atrazine and trifluoroacetate (tfa) were studied in early life stages of the zebrafish (danio rerio). furthermore, the effects of these xenobiotics on soluble (s) and microsomal (m) glutathione s-transferases (gst) of zebrafish embryos were investigated using 1-chloro-2,4-dinitrobenzene (cdnb), 1,2-dichloro-4-nitrobenzene (dcnb), and [(14)c]atrazine. [(14)c]atrazine was taken up by the embryos within seconds, unhindered by the chorions. it accumulated in ... | 2000 | 10648131 |
| the effect of partial removal of yolk on the chilling sensitivity of zebrafish (danio rerio) embryos | the effect of partial removal of yolk on the survival of zebrafish embryos and the chilling sensitivity of yolk-reduced embryos were investigated at several stages of embryo development. dechorionated embryos were punctured with a sharp microneedle and approximately 50 to 75% of yolk content was released following multiple punctures. the survival of yolk-reduced embryos was found to be stage dependent. only 7.9% of 26-somite (24 h) embryos survived, whereas 56.7% of prim-6 (27 h), 62.4% of prim- ... | 1999 | 10600257 |
| ole e i: epitope mapping, cross-reactivity with other oleaceae pollens and ultrastructural localization. | ole e i is the major allergen derived from olive tree pollen (olea europaea) and it is composed of two polypeptides with molecular weights (mws) of 18 and 20 kd. a panel of six monoclonal antibodies (mabs) has been prepared and used to map antigenic determinants on this molecule. four epitope determinants have been identified on ole e i. using the purified mabs produced against ole e i, we have analyzed the common epitope determinants in olive (o. europaea) and different oleaceae pollens: ash (f ... | 1994 | 7515294 |
| fundamental host range of leptoypha hospita (hemiptera: tingidae), a potential biological control agent of chinese privet. | chinese privet, ligustrum sinense lour., is an invasive shrub within riparian areas of the southeastern united states. biological control is considered the most suitable management option for chinese privet. the potential host range of the lace bug, leptoypha hospita drake et poor, was evaluated on the basis of adult feeding and oviposition, combined oviposition-nymphal development no-choice tests, nymphal development no-choice tests, multiple generation comparison on forestiera pubescens nutt. ... | 2016 | 27325627 |
| functional and cell proliferative properties of an exopolysaccharide produced by nitratireductor sp. prim-31. | exopolysaccharides (eps) produced by bacteria are important source of natural biomolecules with therapeutic applications. in this study eps produced by a strain prim-31 isolated from seawater from south west coast of india identified by 16s rrna gene sequencing as nitratireductor sp. was investigated for its biotechnological applications. the isolate produced 650 mg l(-1) eps under optimum growth conditions. the purified eps contained 58.3% carbohydrates with 7.08% uronic acid containing sugars, ... | 2016 | 26772917 |