| three species of land leeches from taiwan, haemadipsa rjukjuana comb. n., a new record for haemadipsa picta moore, and an updated description of tritetrabdella taiwana (oka). | three species of land leeches, including a new combination haemadipsa rjukjuanacomb. n., a new record for haemadipsa picta moore, as well as an updated description for tritetrabdella taiwana (oka), are reported in this study. morphological characters and dna barcode analysis were used to identify these species. in addition, since haemadipsa rjukjuana had been regarded as a variety of the japanese land leech haemadipsa japonica for a century, morphological differences between these two species we ... | 2011 | 22259307 |
| keeping leeches at bay: field evaluation of plant-derived extracts against terrestrial blood-sucking leeches (haemadipsidae) in lao pdr. | terrestrial blood-sucking leeches (haemadipsidae) are common in the damp forests of the subtropical and tropical indo-pacific region. members of the genus haemadipsa are abundant in laos and adjacent countries of southeast asia, and discomfort to people and livestock. plant-derived repellents against arthropods and leeches are common in lao pdr, and have been used by lao ethnic groups for generations. numerous studies have been conducted on the efficacy of traditional plant-derived repellents ag ... | 2011 | 21679682 |
| a new lineage of trypanosomes from australian vertebrates and terrestrial bloodsucking leeches (haemadipsidae). | little is known about the trypanosomes of indigenous australian vertebrates and their vectors. we surveyed a range of vertebrates and blood-feeding invertebrates for trypanosomes by parasitological and pcr-based methods using primers specific to the small subunit ribosomal rna (ssu rrna) gene of genus trypanosoma. trypanosome isolates were obtained in culture from two common wombats, one swamp wallaby and an australian bird (strepera sp.). by pcr, blood samples from three wombats, one brush-tail ... | 2005 | 15777919 |
| the complete amino acid sequences of four globins from the land leech haemadipsa zeylanica var. japonica. | the amino acid sequences of four globins from the land leech, haemadipsa zeylanica var. japonica, were determined using nucleotide sequencing and protein sequencing. the mature globin-molecules were composed of 146 amino acid residues for m-1 globin, 156 for m-2 globin, 143 for d-1 globin, and 149 for d-2 globin. alignment of the four kinds of globins by clustal x revealed 22 invariant amino acids. the four globins were 26-33% identical. a striking feature of amino acid alteration was: the repla ... | 2004 | 15081996 |
| leech hemoglobin: primary structures of four kinds of globins from haemadipsa zeylanica var. japonica. | | 2004 | 15036314 |
| observations on the behaviour of land-leeches (genus haemadipsa). | | 1950 | 14785963 |
| characterization of the residues involved in the human alpha-thrombin-haemadin complex: an exosite ii-binding inhibitor. | haemadin is a 57-amino acid thrombin inhibitor from the land-living leech haemadipsa sylvestris, whose structure has recently been determined in complex with human alpha-thrombin. here we communicate the effect of ionic strength on the kinetics of the inhibition of human alpha-thrombin by haemadin, by using thrombin mutants modified in exosite ii. data analysis has allowed both the ionic and nonionic binding contributions to be ascertained, with the nonionic component being virtually the same fo ... | 2002 | 11851400 |
| [toxicity of different pesticides to haemadipsa hainana and its control]. | laboratory determination on the toxicity of sumicidin, isoprocarb, dicofol, thiocyclam, dimethoate and glyphosate to haemadipsa hainana showed that ld50 for the six pesticides followed the order of sumicidin > isoprocarb > dicofol > thiocyclam > dimethoate, but glyphosate was not virulent. "glyphosate + sumicidin" had much more toxicity. the susceptibility of h. hainana to sumicidin differed in the order of adult > juvenile > subadult. field tests showed that the control effect of 1.5% sumicidin ... | 2001 | 11757377 |
| crystal structure of the human alpha-thrombin-haemadin complex: an exosite ii-binding inhibitor. | the serine proteinase alpha-thrombin plays a pivotal role in the regulation of blood fluidity, and therefore constitutes a primary target in the treatment of various haemostatic disorders. haemadin is a slow tight- binding thrombin inhibitor from the land-living leech haemadipsa sylvestris. here we present the 3.1 a crystal structure of the human alpha-thrombin- haemadin complex. the n-terminal segment of haemadin binds to the active site of thrombin, forming a parallel beta-strand with residues ... | 2000 | 11060016 |
| leech extracellular hemoglobin: two globin strains that are akin to vertebrate hemoglobin alpha and beta chains. | leech (whitmania edentula, haemadipsa zeylanica var. japonica and erpobdella lineata) extracellular hemoglobins are basically composed of three constituent subunits, a dimer (d1 and d2 chains) and two monomers (m1 and m2 chains). we isolated these four chains from respective species by a combination of reversed-phase chromatography on a resource rpc column and gel-filtration on a superdex 75 column. the apparent molecular masses of the four globin chains were estimated by sds-page analysis to be ... | 1997 | 9520633 |
| thrombin inhibitors of bloodsucking animals. | hematophagous animals have the unique ability to inhibit blood coagulation when sucking blood from a wound. this article concerns thrombin inhibitors from these animals, specifically the one from the insect rhodnius prolixus. to date, the most-studied inhibitor from these animals is hirudin, which specifically neutralizes thrombin but no other clotting serine proteases. the biochemical properties of hirudin are described. hirudinlike thrombin inhibitors from the asian leech hirudo manillensis, h ... | 1996 | 8807718 |
| isolation, sequence analysis, and cloning of haemadin. an anticoagulant peptide from the indian leech. | a slow, tight-binding inhibitor of thrombin with an apparent molecular mass of about 5 kda has been isolated from haemadipsa sylvestris, an indian leech of the family of haemadipsidae. the inhibitory activity, called haemadin, is thrombin specific since it does not inhibit other proteases like trypsin, chymotrypsin, factor xa, or plasmin. nh2-terminal amino acid sequence analysis (residues 1-45) does not reveal any homology to known serine protease inhibitors, including the thrombin-specific inh ... | 1993 | 8473305 |
| histochemical localization of alkaline phosphatase in the testicular nephridia of the land-leech, haemadipsa sp. | | 1968 | 5670276 |
| laboratory and field tests of the effectiveness of the lemon-eucalyptus extract, citridiol, as a repellent against land leeches of the genus haemadipsa (haemadipsidae). | citridiol is an extract of the leaves of corymbia citriodora (myrtaceae), the lemon eucalyptus, and mostly consists of p-menthane-3,8-diol isomers. the effectiveness of this extract as a repellent against land leeches of the genus haemadipsa (haemadipsidae), primarily h. sylvestris, was tested in the laboratory and field, in peninsular malaysia. the formulation tested, mosi-guard natural spray, contained 40% (w/w) citridiol in a base of ethanol, water and isopropanol. in the laboratory test, spe ... | 2005 | 16212803 |