| [the origin of the locomotor and trophic activities in the young wolf-fish dicentrarchus labrax (l.) (pisces perranidae)]. | the spontaneous activities of a fixed type shown by the larvae of d. l. are at the beginning random (at least apparently) and not directed. the rheotactism which appears as soon as the eyes are pigmented has been used for the presentation of lures, thus allowing the study of the stimuli releasing the feeding activity and the breeding of 913 individuals up to the alevin stage. | 1975 | 808350 |
| the comparison of the effects of heavy metal ions on the antioxidant enzyme activities in human and fish dicentrarchus labrax erythrocytes. | 1. the effect of the increasing concentrations of cuso4 and hgcl2 (0.01-0.3 mmol/l) on erythrocyte haemolysis and the activities of peroxide metabolism enzymes: superoxide dismutase, catalase, peroxidase and glutathione peroxidase was investigated in human erythrocytes and the nucleated red blood cells of marine fish (dicentrarchus labrax). 2. the results show that both heavy metal ions had only little effect on haemolysis and antioxidant enzyme activities in human erythrocytes; in contrast the ... | 1992 | 1358529 |
| the influence of polyunsaturated fatty acids on spermine inhibition of lipoperoxidation. studies on liposomes prepared with microsomal and mitochondrial phospholipids of sea bass (dicentrarchus labrax l.) and rat liver. | 1. composition of phospholipids extracted from different organelles of european sea bass liver was determined and compared with that of phospholipids extracted from the same organelles of rat liver. 2. spermine binding to the vesicles prepared from microsomal and mitochondrial phospholipids and their aggregation was studied: these parameters indicate that only the presence of acidic phospholipids and not their unsaturation was essential for polyamine action. 3. no correlation exists between poly ... | 1989 | 2758801 |
| the attachment of the monogenean diplectanum aequans (wagener) diesing to the gills of morone labrax l. | | 1966 | 5968798 |
| ovarian melanotropic peptides and adaptation in two teleostean species: sparus aurata l. and dicentrarchus labrax l. | the ovarian tissue of dicentrarchus labrax and sparus aurata displays two immunoreactive peaks that correspond to the elution time of human des-acetyl alpha-msh [acth(1-13)-amide] and human alpha-msh. in view of the close identity between the primary structure of fish and human alpha-msh, these data demonstrate that two msh-related peptides are present both in sea bream and sea bass ovary. alpha-msh-like immunoreactivity was found within both granulosa and thecal layers of mature follicles, as w ... | 1994 | 7984515 |
| cloning and characterization of the european seabass, dicentrarchus labrax, mitochondrial genome. | mitochondrial dna (mtdna) from the european seabass, dicentrarchus labrax, has been cloned and characterized. its gene organization was deduced by a comparison of the sequenced termini of different subclones obtained from european seabass mtdna to the completely-sequenced mtdnas from carp and freshwater loach. the difference in genome size between the european seabass mtdna (approximately 18 kb) and most of the other characterized fish mtdnas (approximately 16.5 kb) is accounted for by the displ ... | 1994 | 8001168 |
| detection and identification of a pathogenic marine mycobacterium from the european seabass dicentrarchus labrax using polymerase chain reaction and direct sequencing of 16s rdna sequences. | mycobacteriosis has become a major concern for the commercial mariculture of the european sea bass dicentrachus labrax in israel. the disease remains asymptomatic for a long time, is virtually impossible to eradicate with antibiotics, stunts the growth of the fish and renders the fish unmarketable. the pathogen was identified as mycobacterium marinum by direct sequencing and analysis of approximately 600 bp of the pathogen ribosomal encoding dna (rdna). the polymerase chain reaction technique wa ... | 1993 | 8293073 |
| factors influencing phagocytic response of macrophages from the sea bass (dicentrarchus labrax l.): an ultrastructural and quantitative study. | although phagocytosis is considered the main nonspecific defense mechanism in fish, the significance of the influence of bacterial properties and assay conditions on this mechanism has not been established. we report the influence of leukocyte source, bacterial species, presence or absence of a bacterial wall, bacterial status (live or dead), and bacterial opsonization on in vitro phagocytosis by sea bass (dicentrarchus labrax l.) macrophages. | 1997 | 9268142 |
| pharmacokinetics of oxolinic acid in sea-bass, dicentrarchus labrax (l., 1758), after a single rapid intravascular injection. | the pharmacokinetics of oxolinic acid was studied in sea-bass (dicentrarchus labrax). the fish were kept in seawater at 15.2 degrees c with a 12 h/12 h photoperiod. oxolinic acid was injected in the caudal vein of anaesthetized sea-bass in a single rapid intravascular administration at a dose of 10 mg/kg of body weight. plasma concentrations of oxolinic acid were determined using two analytical methods, a classic plate diffusion bioassay using escherichia coli and a high performance liquid chrom ... | 1997 | 9280366 |
| primary culture of gill epithelial cells from the sea bass dicentrarchus labrax. | we have developed the first explant technique that allows the in vitro study of gill physiology and biochemistry in marine species. gill fragments were cultured at 17 degrees c, in atmospheric pco2, with nutrient medium (leibovitz l15), ph 7.8, supplemented with 10% fetal bovine serum and adjusted to the osmolarity of fish plasma (350 mosm/liter). coating plates with collagen, gelatin, or polylysin did not improve our results. decrease in osmotic pressure, removal of bovine serum, or its replace ... | 1994 | 8193773 |
| immunohistochemical characterization of a polyclonal antibody against sphaerospora dicentrarchi (myxosporea: bivalvulida), a parasite from sea bass (dicentrarchus labrax l.) (teleostei: serranidae). | rabbit antibody was raised against sphaerospora dicentrarchi, a histozoic parasite of sea bass (dicentrarchus labrax l.). light and electron immunohistological staining were used to characterize its specificity and possible reactivity toward other fish parasites. in light immunohistochemistry the polar capsules and valves of the s. dicentrarchi spores appeared strongly stained, whereas developmental stages were not. electron microscopic histochemistry revealed intense labeling in valves and some ... | 1998 | 9766902 |
| non-specific cytotoxic response against tumor target cells mediated by leucocytes from seawater teleosts, sparus aurata and dicentrarchus labrax: an ultrastructural study. | the in vitro cytotoxic reaction of leucocytes from the seawater teleosts gilthead seabream (sparus aurata) and sea bass (dicentrarchus labrax) against tumor target cells was studied by transmission and scanning electron microscopy. head-kidney, blood or peritoneal exudate leucocytes were incubated with hela or b16 melanoma cells. after incubation, conjugates of leucocytes (effectors) and tumor cells (targets) were observed. both the effector-target cell binding and target cell lysis steps of the ... | 1994 | 7880589 |
| phagocytic defence mechanism in sea bass (dicentrarchus labrax l.): an ultrastructural study. | the ultrastructure of the phagocytic process in fish has not been established in spite of the significant morphofunctional differences detected in the fish immune system with respect to the basic immunological pattern in vertebrates. we report the ultrastructure of the bacterial phagocytic defence mechanism in sea bass (dicentrarchus labrax l.). | 1994 | 7879910 |
| the prolactin of european sea bass (dicentrarchus labrax l.): cloning of cdna and efficient expression in escherichia coli. | the cdna encoding sea bass (dicentrarchus labrax) prolactin (sbprl) was obtained by reverse transcription-polymerase chain reaction (rt/pcr) from pituitary rna with degenerate primers designed on the basis of the cdnas of the two prls (tprl188 and tprl177) from the tilapia, oreochromis niloticus. the sbprl cdna encodes a preprotein of 212 amino acids composed of a putative signal peptide of 24 residues and a mature protein of 188 amino acids that is the homologue of tiprl188. the cdna coding for ... | 1994 | 7804137 |
| isolation and properties of white skeletal muscle alpha-actinin from sea-trout (salmo trutta) and bass (dicentrarchus labrax). | fish alpha-actinin purified from sea-trout and bass white muscle by means of two different extraction procedures was used to investigate the eventual presence of different muscle isoforms in z-disks. these fish alpha-actinins have the same apparent molecular weight (100 kda) and the same isoelectric point (pi = 5.6), and also have a total antigenic identity towards anti-bass and anti-chicken alpha-actinin antibodies, suggesting a single molecular species. the role of fish alpha-actinin as an anc ... | 1995 | 7584856 |
| determination of glucose turnover in sea bass dicentrarchus labrax. comparative aspects of glucose utilization. | 1. parameters of in vivo glucose utilization by sea bass (132 +/- 6 g, mean +/- sem) acclimated at 15 degrees c in sea-water were measured after single injection of labelled glucose. 2. glucose turnover rate (rg; mumol . min-1 . kg-1) was found to be 0.55-065 (2-3h glucose) and 0.34 +/- 0.42 (u14c glucose). 3. glucose transit time was 443-449 min, glucose mass 233-261 mumol . kg-1, and glucose recycling 37%. 4. oxygen consumption (mo2) amounted to 94 +/- 6.2 mumol . min-1 . kg-1. 5. the comparis ... | 1987 | 3665443 |
| polyamine distribution and activity of their biosynthetic enzymes in the european sea bass (dicentrarchus labrax l.) compared to the rat. | 1. in the liver, heart and brain of the european sea bass, putrescine concentrations are much higher than in the equivalent rat tissues; spermidine and spermine levels are smaller. 2. ornithine decarboxylase in the bass liver is more active, but less stable than that in the rat; stability is acquired upon partial purification. bass liver adenosylmethionine decarboxylase activity is less than that found in the rat. both are activated and stabilized by putrescine. 4. the activating effect of putre ... | 1987 | 3427897 |
| localization of growth hormone releasing factor-like immunoreactivity in the hypothalamo-hypophyseal system of the frog (rana temporaria) and the sea bass (dicentrarchus labrax). | using two different antisera, one raised against total human pancreatic growth hormone releasing factor (hpgrf) coupled through a two-step glutaraldehyde method and the other one raised against rat hypothalamic growth hormone releasing factor 1-10 (rgrf1-10), grf-like immunoreactivity was localized in the hypothalamo-hypophyseal system of the frog (rana temporaria) and the sea bass (dicentrarchus labrax). in the frog immunoreactive neurons were found in the nucleus preopticus, pars magnocellular ... | 1988 | 3141244 |
| spontaneous cytotoxic activity of eosinophilic granule cells separated from the normal peritoneal cavity of dicentrarchus labrax. | in this study the spontaneous in vitro cytotoxic activity to tumour cell lines, (k562), by unstimulated sea bass (dicentrarchus labrax) leukocytes was examined by trypan blue exclusion test and lactate dehydrogenase release assay. a high anti-tumour cell line activity of resident peritoneal leukocytes was found at an effector to target ratio (e:t) of 25:1 after incubation for 2 h at 18 degrees c. rabbit and sheep erythrocytes were not lysed. a low activity was displayed by head kidney and spleen ... | 2000 | 10938730 |
| plasma essential amino acids changes in sea-bass (dicentrarchus labrax) after feeding diets deficient and supplemented in l-methionine. | a fishmeal-based reference diet, a soybean-yeast-based methionine (met)-deficient diet and two met-supplemented diets were fed to groups of yearling sea-bass, reared in sea-water at 21 +/- 1 degrees c. levels of most free essential amino acids (eaa) reached a maximum concentration in plasma between 4 and 8 hr after a single meal. plasma met level remained very low with the deficient diet, exhibiting by 4 hr a 10-fold reduction when compared to the reference diet, although met content was divided ... | 1985 | 2864207 |
| granulopoiesis in the head-kidney of the sea bass (dicentrarchus labrax l.): an ultrastructural study. | the ultrastructure of granulopoiesis in the head-kidney of the sea bass (dicentrarchus labrax l.), an euryhaline seawater teleost, was studied. the heterophilic, acidophilic and basophilic series consisting of promyelocyte, myelocyte, metamyelocyte and mature granulocyte stages are described. the granulopoietic pattern was similar to those of higher vertebrates but dissimilar to those of some other fish, suggesting that the vertebrate granulopoietic scheme was probably conserved during the evolu ... | 1990 | 2390328 |
| the uptake metabolism and biological half-life of benzo[a]pyrene in different tissues of sea bass, dicentrarchus labrax. | the disposition of polycyclic aromatic hydrocarbons in aquatic organisms is of interest from the viewpoint of both animal and human health. the kinetics of uptake and metabolism of benzo[a]pyrene (bap) were studied in various tissues of the marine fish, sea bass, upon intraperitoneal injection of the compound. a decrease in radioactivity occurred in all tissues and the calculated half-lives were 12.4 days for fat, 6.5 for kidney, 5.1 for intestine, 4.8 for gallbladder, 4.5 for spleen, 2.9 for mu ... | 1990 | 2090438 |
| viral erythrocytic infection in sea bass: virus purification and confirmative diagnosis. | the viral erythrocytic infection (vei) of sea bass (dicentrarchus labrax) is evidenced by the presence of inclusion bodies in the erythrocytes and erythroblasts. virus-like particles (veiv) about 135-150 nm in diameter were observed in erythroblasts of affected fish. no culturable virus could be recovered from the organs and blood of vei-affected fish, after inoculation of different fish cell lines. histochemical staining revealed the presence of rna but not of dna in the inclusion bodies. speci ... | 1991 | 1929880 |
| immunoaffinity purification and partial characterization of sea bass (dicentrarchus labrax) growth hormone. | growth hormone (gh) was isolated from sea bass (dicentrarchus labrax) pituitary extract by a simple one-step procedure involving immunoaffinity chromatography. a monoclonal antibody raised against chicken gh and found to immunostain very specifically the gh cells in the pituitary of the sea bass was coupled to cnbr-activated sepharose 4b. sea bass pituitary extracts were run on the affinity column, and the eluted material was analyzed on reversed-phase hplc and found to consist of one single pea ... | 1991 | 1916214 |
| diplectanidae (monogenea) infestations of sea bass, dicentrarchus labrax (l.), from the spanish mediterranean area. histopathology and population dynamics under culture conditions. | in the present study, diplectanum laubieri was redescribed from material obtained from wild and cultured fish. the population dynamics of d. aequans and d. laubieri under culture conditions was studied. the prevalence and mean intensity (average number of worms per parasitized fish) were 80.64% and 112 for d. aequans and 67.74% and 59.61 for d. laubieri, respectively. both diplectanidae exhibited seasonal variations, with maximal infection levels occurring in winter, suggesting continuous parasi ... | 1991 | 1866420 |
| pancreatic endocrine cells in sea bass (dicentrarchus labrax l.) ii. immunocytochemical study of insulin and somatostatin peptides. | insulin (ins)- and somatostatin (sst)-immunoreactive cells were demonstrated by light immunocytochemistry in the endocrine pancreas of sea bass (dicentrarchus labrax). ins-immunoreactive cells were identified using bovine/porcine, bonito, and salmon (s) ins antisera; the immunostaining was abolished when each antiserum was preabsorbed with its respective peptide but not with unrelated peptides. these cells also reacted with mammal (m) sst-28 (4-14) antiserum. the immunoreaction did not change wh ... | 1991 | 1673443 |
| molecules, morphology and morphometrics of cainocreadium labracis and cainocreadium dentecis n. sp. (digenea: opecoelidae) parasitic in marine fishes. | molecular, morphological and morphometric analyses were conducted on several samples of cainocreadium labracis (opecoelidae), a trematode parasitic in marine teleosts. the samples were isolated from several specimens of dicentrarchus labrax, the type host, and dentex dentex. the molecular analysis of complete internal transcribed spacer sequences of ribosomal dna revealed that specimens isolated from each host species form two well-defined groups, whose sequence divergence reaches 7.5%. the morp ... | 2001 | 11336752 |
| cloning and sequencing of european sea bass (dicentrarchus labrax l.) growth hormone cdna using polymerase chain reaction and degenerate oligonucleotides. | to clone and sequence the cdna of the growth hormone of european sea bass (dicentrarchus labrax l.) (sbgh), total pituitary rna was reverse transcribed and amplified using polymerase chain reaction (pcr). degenerate oligonucleotides, designed by comparing available gh cdna sequences from related teleost species, were used as primers to amplify the 5' end and the core region of sbgh cdna, while the 3' end was amplified according to the rapid amplification of cdna ends (race) method. sbgh cdna con ... | 1992 | 1472711 |
| [gill repartition of two monogenea: diplectanum aequans (wagener 1857), diesing 1958 and d. laubieri lambert and maillard 1974 (monogenea, monopisthocotylea) simultaneous parasites of the sea bass dicentrarchus labrax (author's transl)]. | the close observation of the gill habitat of two monogeneans diplectanum aequans (wagener 1857) diesing 1858 and diplectanum laubieri lambert and maillard 1974 shows the microbiotopes of these two simultaneous parasites of the sea-bass: dicentrarchus labrax. the authors propose to study the distribution of the monogeneans with references to three theoretic axes in the gill chambers. for the diplectanum, the populations spread on each of these axes according to preferential gradients. | 1975 | 1227377 |
| recent advances in our knowledge of the myxozoa. | in the last few years two factors have helped to significantly advance our understanding of the myxozoa. first, the phenomenal increase in fin fish aquaculture in the 1990s has lead to the increased importance of these parasites; in turn this has lead to intensified research efforts, which have increased knowledge of the development, diagnosis. and pathogenesis of myxozoans. the hallmark discovery in the 1980s that the life cycle of myxobolus cerebralis requires development of an actinosporean s ... | 2001 | 11456316 |
| action of leptin on in vitro luteinizing hormone release in the european sea bass (dicentrarchus labrax). | the discovery of leptin has sparked a rapidly growing number of publications concerning the role of leptin in the regulation of body adiposity, feeding, and reproductive system in mammals. to date, there have been no reports on the presence of leptin-related peptide, and functional studies on the role of leptin remain limited in fishes. we investigated the effect of mouse recombinant leptin on basal and sea bream (sb) gnrh-induced lh release from dispersed pituitary cells obtained from male euro ... | 2001 | 11673277 |
| [phyletic affinities among polystomatidae (monogenea)]. | the chaetotaxy and ciliated cells in two monogenean larvae of marine fishes are described for the first time: a monopisthocotylea diplectanum aequans (wagener, 1857) diesing, 1858 parasite of dicentrarchus labrax (l) and a polyopisthocotylea, microcotyle mormyri lorenz, 1878 parasite of pagellus mormyrus (l). a comparative study with the known larvae shows evidence of two larval types in the monogenea of fishes, related to the two great phyla of the class: the monopisthocoytylea and polyopisthoc ... | 1977 | 413661 |
| glucagon- and npy-related peptide-immunoreactive cells in the gut of sea bass (dicentrarchus labrax l.): a light and electron microscopic study. | glucagon and peptide of the neuropeptide y (npy) family immunoreactivities were studied in the gut of sea bass (dicentrarchus labrax) using antisera against bovine/porcine glucagon, porcine glucagon, glicentin (10-30), bovine pancreatic polypeptide (pp), peptide tyrosine tyrosine (pyy), salmon pyy (spyy), and npy. glucagon-, glicentin-, pyy-, and npy-immunoreactive (ir) cells were detected in the stomach, and glucagon-, pp-, pyy-, spyy-, and npy-ir cells in the intestine. pp, pyy, and npy immuno ... | 1998 | 9748400 |
| adrenocortical and adrenomedullary homologs in eight species of adult and developing teleosts: morphology, histology, and immunohistochemistry. | morphology, histology, and immunohistochemistry of the adrenocortical and adrenomedullary homologs (adrenal glands) of the following developing and adult teleosts were examined: salmoniformes-oncorhynchus mykiss (rainbow trout), salmo trutta fario (brown trout), coregonus lavaretus (white fish); cyprinodontiformes-gambusia affinis (mosquito fish). perciformes-dicentrarchus labrax (sea bass), sparus aurata (sea bream), diplodus sargus (white bream), oblada melanura (saddled bream). the anatomical ... | 1997 | 9405124 |
| cloning, expression and purification of the coat protein of encephalitis virus (diev) infecting dicentrarchus labrax. | the coat protein gene from encephalitis virus infecting dicentrarhus labrax (diev) has been cloned by gene amplification, sequenced and expressed in escherichia coli. dna sequencing has revealed an open reading frame of 1017 bases encoding a polypeptide of 338 amino acids. the sequence similarities between the diev coat protein gene and the same gene in five encephalitis viruses infected other fish species were over 71.5% at the nucleotide level and over 79.5% at the amino acid level. these resu ... | 1997 | 9238540 |
| development of enzyme immunoassays for 3,5,3'-triiodo-l-thyronine and l-thyroxine: time-course studies on the effect of food deprivation on plasma thyroid hormones in two marine teleosts, sea bass (dicentrarchus labrax l.) and sea bream (sparus aurata l.). | the effects of short-term food deprivation and photoperiod on plasma thyroid hormone levels of sea bass and sea bream were studied. animals were acclimated under constant photoperiod regime (15l/9d) and feeding times (2 hr after light onset and 2 hr before light offset). time-course studies involved monitoring plasma hormone levels every 4 hr throughout 1.5 24-hr cycles. plasma 3,5, 3'-triiodo-l-thyronine (t3) and l-thyroxine (t4) were assayed using a newly developed competitive enzyme immunoass ... | 1996 | 8812399 |
| immunotoxic effects of copper and cadmium in the sea bass dicentrarchus labrax. | two phagocytes-mediated activities of the sea bass dicentrarchus labrax were examined after exposure to sublethal concentrations of copper and cadmium: (a) phagocytosis (measured by phagocytotic index), and (b) the production of reactive oxygen intermediates (luminol-dependent chemiluminescence) in response to bacteria aeromonas salmonicida. in vivo exposure for 48 h to each metal separately by intraperitoneal injection did not affect the quantity of phagocytes of pronephros and their viability ... | 1996 | 8683034 |
| population dynamics of sphaerospora dicentrarchi sitja-bobadilla et alvarez-pellitero, 1992 and s. testicularis sitja-bobadilla et alvarez-pellitero, 1990 (myxosporea: bivalvulida) infections in wild and cultured mediterranean sea bass (dicentrarchus labrax l.). | the population dynamics of sphaerospora dicentrarchi and s. testicularis, myxosporean parasites of sea bass (dicentrarchus labrax), was studied in different groups of cultured and wild fish. s. dicentrarchi prevalence was higher for wild fish (100%) than for cultured ones (70.5%). infection levels increased with host age and there were no significant sex differences in cultured fish. among parasitized organs, the gall bladder and intestine registered the highest infection levels in wild and cult ... | 1993 | 8479799 |
| photobacterium damselae ssp. piscicida: detection by direct amplification of 16s rrna gene sequences and genotypic variation as determined by amplified fragment length polymorphism (aflp). | a pcr protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16s rrna gene sequences was developed. the procedure combines low annealing temperature that detects low titers of photobacterium damselae but also related species, and high annealing temperature for the specific identification of p. damselae directly from infected fish. the pcr protocol was validated on 19 piscine isolates of p. damselae ssp. piscicida from different geographic regions (japan, italy, spain, greece and isra ... | 2002 | 12033705 |
| molecular cloning of a phthalate-inducible cyp4 gene (cyp4t2) in kidney from the sea bass, dicentrarchus labrax. | a full-length cdna sequence was isolated from kidney total rna of di(2-ethylhexyl) phthalate-treated sea bass by reverse-transcriptase polymerase chain reaction and then rapid amplification of cdna ends. the deduced amino acid sequence, which has been named cyp4t2, shared 69 and 54.4% amino acid identity with rainbow trout cyp4t1 and rat cyp4b1, respectively. rna blot analysis using the cyp4t2 cdna as a probe indicated that the mrna was rather abundant in kidney, and less so in liver, small inte ... | 1998 | 9790933 |
| dietary fish oil and digestible protein modify susceptibility to lipid peroxidation in the muscle of rainbow trout (oncorhynchus mykiss) and sea bass (dicentrarchus labrax). | the effects of dietary fish oil and digestible protein (dp) levels on muscle fatty acid composition and susceptibility to lipid peroxidation were studied in two representative fish species for human nutrition, from fresh and seawater, rainbow trout (oncorhynchus mykiss) and european sea bass (dicentrarchus labrax). in rainbow trout, higher concentrations of dietary fat and dp led to higher weight gain (g/d) (p = 0.001 and p = 0.043 respectively). additionally, an interaction effect was observed ... | 1998 | 9875068 |
| cloning the neuropeptide y exon 2 from sea bass (dicentrarchus labrax). | a partial genomic library of sea bass dna was constructed and screened with a goldfish npy cdna probe. two identical clones were isolated and sequenced. the clones contain a segment with high identity to exon 2 of the npy gene in tetrapods. this segment encodes a 62-amino acid peptide consisting of a signal peptide of 28 amino acids and the main portion of the mature npy (34 amino acids). in the latter extension, sea bass npy shows high identity with the human and deduced ancestral gnathostome s ... | 1999 | 10425721 |
| viral encephalopathy and retinopathy of farmed marine fish species in italy. | viral encephalopathy and retinopathy, otherwise known as fish encephalitis or viral nervous necrosis (vnn), is an emerging problem in several farmed marine fish species in various geographic areas all over the world. since summer 1995, heavy losses affecting mainly juvenile and adult sea bass (dicentrarchus labrax) have been observed in several on-growing facilities in italy. dying fish show abnormal swimming behaviour and, at temperatures higher than 20-22 degrees c, mortality rates range betwe ... | 1999 | 10509726 |
| energetic dependence of npy-induced lh secretion in a teleost fish (dicentrarchus labrax). | the purpose of this work was to examine the role of energetic status in neuropeptide y (npy)-induced luteinizing hormone (lh) secretion and glucose metabolism in fish. fasted juvenile sea bass (dicentrarchus labrax) were injected intraperitoneally with pig (p) npy or pnpy + glucose, whereas fed animals were injected with pnpy alone and plasma glucose, insulin, and lh levels were examined. pnpy alone or in combination with glucose was found to induce a dose-dependent increase in lh secretion in f ... | 1999 | 10600908 |
| first record of emetha audouini, a cymothoid isopod parasite, from cultured sea bass dicentrarchus labrax in greece. | for the first time, emetha audouini (milne edwards, 1840), a cymothoid isopod, is reported parasitising cage cultured sea bass dicentrarchus labrax l., 1758 in greece. the specimens observed are larvae (pulli ii). they were found in great numbers in the buccal and branchial cavity of young (3.5 m.o. old) sea bass, in an intensive cage farm facility. this parasite is certainly transferred to sea bass from wild populations of sparidae or centracanthidae. serious lesions were visible and typical of ... | 1999 | 10686675 |
| mycobacteriosis in wild rabbitfish siganus rivulatus associated with cage farming in the gulf of eilat, red sea. | infection patterns of mycobacterium marinum were studied over a period of 3 yr in wild rabbitfish siganus nivulatus populations associated with commercial mariculture cages and inhabiting various sites along the israeli red sea coastline. mycobacteriosis was first recorded from the red sea in 1990 in farmed sea bass dicentrarchus labrax and is absent from records of studies on parasites and diseases of wild rabbitfish carried out in the 1970s and 1980s. a sharp increase in the prevalence of the ... | 2000 | 10768289 |
| immunohistochemical localization of s100-like protein in non-mammalian kidney. | the immunolocalization of s100-like protein was investigated in the kidney of saltwater fishes (dicentrarchus labrax; coris julis; serranus cabrilla; scorpaena porcus), amphibia (rana aesculenta), reptiles (lacerta viridis), and aves (gallus domesticus; strutio camelus). s100-like immunoreactivity was detected in the juxtaglomerular cells of all saltwater fishes studied. no immunoreactivity was observed in other tracts of the nephron or in the interstitial tissue. in frog kidney, s100-like immun ... | 2003 | 12645012 |
| [gonadal ontogenesis and sex differentiation in the sea bass, dicentrarchus labrax, under fish-farming conditions]. | the histology of the different stages of gonadal development (appearance of pgc, edification of gonad primordium, organization of an undifferentiated gonad, testicular or ovarian development) has been studied in fingerlings and juveniles of sea-bass in fish-culture conditions. sex differentiation with a caudo-cranial gradient was direct and more in accordance with length than with age. ovarian and testicular differentiation occurred in fish 11 to 23 months old and from 90 to 187 mm sl. testis ov ... | 1983 | 6844709 |
| antigenic characterization of sphaerospora dicentrarchi (myxosporea: bivalvulida), a parasite from european sea bass dicentrarchus labrax (teleostei: serranidae). | the biochemical composition of sphaerospora dicentrarchi was studied. periodate and proteinase k treatments as well as lectin blots were used to analyse carbohydrate terminals. zymography was applied to detect proteases. four polyclonal antisera, raised against s. dicentrarchi (rasdic), s. testicularis (rastest), ceratomyxa labracis (raclab) and c. sparusaurati (racspr), were used in sds polyacrylamide gel electrophoresis and immunoblot. bands with molecular weight (mw) between 32 and 130 kda we ... | 2000 | 10782345 |
| in vitro effect of leptin on somatolactin release in the european sea bass (dicentrarchus labrax): dependence on the reproductive status and interaction with npy and gnrh. | the aim of the present work was to investigate the neuroendocrine control of pituitary somatolactin (sl) release using dispersed pituitary cell culture obtained from male european sea bass (dicentrarchus labrax) at different stages of sexual development. the effect of mouse recombinant leptin, sea bream gonadotropin releasing-hormone (sbgnrh) and porcine neuropeptide y (pnpy) and their potential interaction on the sl release were investigated. high doses of leptin (10(-8)-10(-6)m) were different ... | 2003 | 12812777 |
| pituitary levels of three forms of gnrh in the male european sea bass (dicentrarchus labrax, l.) during sex differentiation and first spawning season. | in the present study, levels of three gnrh forms [seabream gnrh (sbgnrh), chicken gnrh-ii (cgnrh-ii), and salmon gnrh (sgnrh)] were analyzed in the pituitary of male sea bass during sex differentiation and the first spawning season. plasma levels of gonadotropin (gth-2), testosterone (t), and 11-ketotestosterone (11-kt) were determined during the same periods. all gnrh forms were present in the pituitary. sbgnrh levels were 9-fold higher than cgnrh-ii and 17-fold higher than sgnrh levels. the hi ... | 2000 | 11042012 |
| modulation of the in vitro activity of european sea bass (dicentrarchus labrax l.) phagocytes by the myxosporean parasite sphaerospora dicentrarchi (myxosporea: bivalvulida). | several in vitro studies were performed to study the cellular reaction of european sea bass (dicentrarchus labrax l.) against sphaerospora dicentrarchi. head kidney phagocytes were obtained from parasitised (p) and non-parasitised (np) fish. the production of superoxide anion (o2-), tested by the nbt method, was higher in p than in np fish. the addition of increasing amounts of sea bass serum (sbs) produced a gradual increment of the respiratory burst with sbs from parasitised animals (p-sbs), w ... | 2000 | 11081435 |
| differential expression of three different prepro-gnrh (gonadotrophin-releasing hormone) messengers in the brain of the european sea bass (dicentrarchus labrax). | the expression sites of three prepro-gonadotrophin-releasing hormones (gnrhs), corresponding to seabream gnrh (sbgnrh: ser(8)-mgnrh, mammalian gnrh), salmon gnrh (sgnrh: trp(7)leu(8)-mgnrh), and chicken gnrh-ii (cgnrh-ii: his(5)trp(7)tyr(8)-mgnrh) forms were studied in the brain of a perciform fish, the european sea bass (dicentrarchus labrax) by means of in situ hybridization. the riboprobes used in this study correspond to the three gnrh-associated peptide (gap)-coding regions of the prepro-gn ... | 2001 | 11086295 |
| effects of polyunsaturated fatty acids and prostaglandins on oocyte maturation in a marine teleost, the european sea bass (dicentrarchus labrax). | the effects of the polyunsaturated fatty acids (pufas), arachidonic acid (aa), eicosapentaenoic acid (epa), docosahexaenoic acid (dha), and prostaglandins (pgs) on oocyte maturation were investigated in a marine teleost, the sea bass (dicentrarchus labrax). follicle-enclosed postvitellogenic, preovulatory oocytes were cultured in vitro and maturation was verified by assessing volume increase, lipid droplet coalescence, yolk clarification, and germinal vesicle migration and breakdown. human chori ... | 2001 | 11133697 |
| isolation in cell culture of nodavirus from farmed atlantic halibut hippoglossus hippoglossus in norway. | isolation in cell culture of nodavirus from atlantic halibut hippoglossus hippoglossus suffering from viral encephalopathy and retinopathy (ver) is described. the cell line ssn-1 was inoculated with tissue material from affected juveniles (60 d after first feeding). extensive cytopathic effects (cpe) developed approximately 5 d after inoculation, and were also observed after several passages in the same cell line. cells from infected cultures showed reactivity with an antiserum against sea bass ... | 2000 | 11206733 |
| spontaneous haemolytic activity of atlantic halibut (hippoglossus hippoglossus l.) and sea bass (dicentrarchus labrax) serum. | the spontaneous haemolytic (sh) activity of sera was compared in groups of cultured halibut and sea bass. the optimum assay temperature was determined for each species and different red blood cell donors were tested. the effects of heat inactivation, storage temperature and of different agents like edta, egta, yeast cell components and bacterial lps were compared. halibut sera gave optimum lysis with sheep red blood cells (rbc) at 16 degrees c whereas sea bass sera showed optimum lysis with rabb ... | 2003 | 12941643 |
| the gill is a major organ for antibody secreting cell production following direct immersion of sea bass (dicentrarchus labrax, l.) in a photobacterium damselae ssp. piscicida bacterin: an ontogenetic study. | extremely high numbers of antibody secreting cells (asc) were observed in the gills of sea bass fry immunised at three different age/sizes (initial weight of 0.1, 2 and 5 g) by direct immersion in a photobacterium damselae spp. piscicida bacterin. the relatively low asc production in the head kidney and spleen suggests that the systemic compartment was only slightly stimulated upon immersion vaccination. there was no response of corresponding magnitude in the gut as the one observed in the gills ... | 2001 | 11271603 |
| interaction of actin with the capping protein, capz from sea bass (dicentrarchus labrax) white skeletal muscle. | we have compared the functional properties of capz from fish white skeletal muscle with those of capz from chicken muscle. capz is a heterodimer, which enhances actin nucleation and inhibits the depolymerization process by binding to the barbed ends of microfilaments. here, we report the interaction of capz not only with f-actin, but also with monomeric actin. the affinity of sea bass capz for g-actin estimated by enzyme-linked immunosorbent assay (elisa) was in the microm range. this associatio ... | 2000 | 11281272 |
| sea bass dicentrarchus labrax nervous necrosis virus isolates with distinct pathogenicity to sea bass larvae. | reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (sb1 and sb2) isolated from sea bass collected along the atlantic and mediterranean french coast. the pathogenicity of the virus strains was assigned after detection of the virus by elisa and immunohistochemistry (ihc). the atlantic (sb1) strain was more pathogenic than the mediterranean (sb2) strain during the f ... | 2001 | 11411641 |
| kinetics of juvenile sea bass (dicentrarchus labrax, l.) systemic and mucosal antibody secreting cell response to different antigens (photobacterium damselae spp. piscicida, vibrio anguillarum and dnp). | the elispot assay was used to measure the number of specific antibody secreting cells (asc) induced during the primary and secondary immune responses in the spleen, head kidney and gut of juvenile (5 g) sea bass (dicentrarchus labrax) to bacterial (vibrio anguillarum and photobacterium damselae ssp. piscicida) and hapten dinitrophenyl-conjugated to keyhole limpet haemocyanin (dnp-klh) antigens administered intraperitoneally. high variability among individuals was observed at each sampling day. a ... | 2001 | 11417719 |
| distribution and potential role of cytosolic water-soluble phosphodiesters in fish. | the distribution of water-soluble phosphodiesters (wspdes) visible by nuclear magnetic resonance (nmr) in some intact tissues of rainbow trout (oncorhynchus mykiss walbaum) and in perchloric extracts after partial purification was examined by (31)p nmr spectroscopy. the compounds of interest were serine ethanolamine phosphate (sep), threonine ethanolamine phosphate (tep), glycerophosphorylcholine (gpc), and glycerophosphorylethanolamine (gpe). tep and sep were mostly accumulated in the heart and ... | 2003 | 13130434 |
| cymothoid isopod parasites in aquaculture: a review and case study of a turkish sea bass (dicentrarchus labrax) and sea bream (sparus auratus) farm. | this paper reviews the occurrence of cymothoid isopod parasitism in aquaculture, reports the first case of infection by a cymothoid isopod (ceratothoa oestroides) in turkish aquaculture, and analyses its effects on sea bass dicentrarchus labrax. analyses revealed that c. oestroides negatively affects the weights and lengths of sea bass hosts. these effects have been previously underestimated because host age has not been accounted for. the analysis of condition factors as a means of assessing pa ... | 2001 | 11710552 |
| the gnrh system in the european sea bass (dicentrarchus labrax). | the cdna sequences encoding three gnrh forms, sea bream gnrh (sbgnrh), salmon gnrh (sgnrh) and chicken gnrh ii (cgnrh ii), were cloned from the brain of european sea bass, dicentrarchus labrax. comparison of their deduced amino acid sequences to the same forms in the gilthead sea bream, sparus aurata, and striped bass, morone saxatilis, revealed high homology of the prepro-cgnrh ii (94% and 98% respectively), and prepro-sgnrh (92% to both species). the sbgnrh exhibited dissimilar identities, wit ... | 2002 | 11786378 |
| sea bream sparus aurata, an asymptomatic contagious fish host for nodavirus. | during an epidemiological survey of viral encephalopathy and retinopathy (ver) in diseased sea bass dicentrarchus labrax, a nodavirus isolate was recovered from net pen-reared sea bream sparus aurata harboured in the same farming premises. after the virus was isolated and identified by immunofluorescence on ssn-1 cells, sequence analysis with a pcr product from the t4 region of the capsid protein gene indicated that the virus shared 100% identity with a pathogenic virus strain isolated from sea ... | 2001 | 11797913 |
| european sea bass (dicentrarchus labrax l.) cytochrome p450arom: cdna cloning, expression and genomic organization. | cytochrome p450arom, a key enzyme in the hormonal steroidogenic pathway, mediates the conversion of androgens to estrogens. this work describes the molecular cloning of the cdna encoding the european sea bass (dicentrarchus labrax l.) cytochrome p450arom by means of reverse transcriptase and polymerase chain reaction (rt-pcr) and 5' and 3'-rapid amplification of cdna ends (race) analyses. the cdna is 1822bp in length and encodes a putative protein of 517 amino acids. northern blot analysis revea ... | 2002 | 11867261 |
| immunohistochemical localization of three different prepro-gnrhs in the brain and pituitary of the european sea bass (dicentrarchus labrax) using antibodies to the corresponding gnrh-associated peptides. | the distribution of the cells expressing three prepro-gonadotrophin-releasing hormones (gnrh), corresponding to salmon gnrh (sgnrh), seabream gnrh (sbgnrh), and chicken gnrh-ii (cgnrh-ii) forms, was studied in the brain and pituitary of the sea bass (dicentrarchus labrax) by using immunohistochemistry. to circumvent the cross-reactivity problems of antibodies raised to gnrh decapeptides, we used specific antibodies generated against the different sea bass gnrh-associated peptides (gap): salmon g ... | 2002 | 11932929 |
| developmental expression of three different prepro-gnrh (gonadotrophin-releasing hormone) messengers in the brain of the european sea bass (dicentrarchus labrax). | in this study, we have analyzed the ontogenic expression of three gonadotrophin-releasing hormones (gnrh) systems expressed in the brain of a perciform fish, the european sea bass, using in situ hybridization. the riboprobes used correspond to the gnrh-associated peptide (gap) coding regions of the three prepro-gnrh cdnas cloned from the same species: prepro-salmon gnrh, prepro-seabream gnrh and prepro-chicken gnrh ii. on day 4 after hatching, the first prepro-chicken gnrh-ii mrna-expressing cel ... | 2002 | 12048109 |
| nodavirus infections in israeli mariculture. | viral encephalopathy and retinopathy (ver) infections were diagnosed in five fish species: epinephelus aeneus, dicentrarchus labrax, sciaenops ocellatus, lates calcarifer and mugil cephalus cultured on both the red sea and mediterranean coasts of israel during 1998-2002. spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. with transmission electron microscopy, paracrystalline arrays and pieces of membrane-associated non-enveloped virions measur ... | 2004 | 15291788 |
| mercury chloride effects on the function and cellular integrity of sea bass (dicentrarchus labrax) head kidney macrophages. | the objective of this work was to study mercury chloride effects on the function and integrity of sea bass (dicentrarchus labrax) head kidney macrophages (s-hkm), and to evaluate the response of hgcl2-exposed cells to macrophage activating factor(s) (maf) produced by sea bass head kidney leukocytes. there was considerable variability in the effects of hgcl2 on the production of reactive oxygen species (ros) by s-hkm. when incubated with hgcl2, cells from five out of nine fish tested showed a dec ... | 2004 | 15313514 |
| cryptosporidium molnari n. sp. (apicomplexa: cryptosporidiidae) infecting two marine fish species, sparus aurata l. and dicentrarchus labrax l. | cryptosporidium molnari n. sp. is described from two teleost fish, the gilthead sea bream (sparus aurata l.) and the european sea bass (dicentrarchus labrax l.). the parasite was found mainly in the stomach epithelium and seldom in the intestine. oocysts were almost spherical, with four naked sporozoites and a prominent residuum, and measured 3.23-5.45 x 3.02-5.04 (mean 4.72 x 4.47) microm in the type host, gilthead sea bream (shape index 1-1.17, mean 1.05). sporulation was endogenous, as fully ... | 2002 | 12076630 |
| antiprotozoals effective in vitro against the scuticociliate fish pathogen philasterides dicentrarchi. | the histophagous ciliate philasterides dicentrarchi causes fatal scuticociliatosis in farmed turbot scophthalmus maximus and sea bass dicentrarchus labrax. the present study screened 52 candidate antiprotozoals for activity against this pathogen in vitro. of these compounds, 14 were effective (i.e. killed all ciliates within a 24 h assay period). in descending order of efficacy (minimum lethal concentration 100 to 0.8 ppm), these were niclosamide, oxyclozanide, bithionol sulfoxide, toltrazuril, ... | 2002 | 12113305 |
| intramolecular disulfide bonding is essential for betanodavirus coat protein conformation. | here we report on the conformational changes that are responsible for the appearance of the dicentrarchus labrax encephalitis virus (dlev) coat protein as a doublet in sds-page. wild-type and mutated forms of the coat protein cdna were expressed in e. coli. the study of the resulting recombinant molecules excluded the possibility of the involvement of a precursor autocatalysis mechanism or a ribosomal frameshifting event in the doublet formation. the appearance of the coat protein doublet was fo ... | 2002 | 12185275 |
| amplified fragment length polymorphism (aflp) and biochemical typing of photobacterium damselae subsp. damselae. | the aim of the present study was to characterize subspecifically photobacterium damselae subsp. damselae strains isolated from cultured sparus aurata and dicentrarchus labrax by means of phenotypic and molecular typing techniques (amplified fragment length polymorphism, aflp). | 2002 | 12234352 |
| the professional phagocytes of sea bass (dicentrarchus labrax l.): cytochemical characterisation of neutrophils and macrophages in the normal and inflamed peritoneal cavity. | in order to identify the phagocytic cells of sea bass, the peritoneal leucocyte population of fish injected intraperitoneally with photobacterium damselae subspecies piscicida was studied by light microscopy using cytocentrifuge preparations stained by the antonow technique for peroxidase detection. among the leucocytes present in the peritoneal exudate of the infected fish (macrophages, neutrophils, eosinophilic granular cells, lymphocytes and thrombocytes), macrophages and neutrophils were the ... | 2002 | 12365730 |
| inhibition of cytochrome p450 enzymes by enrofloxacin in the sea bass (dicentrarchus labrax). | currently, there are no reports on the effects of enrofloxacin (ef), a fluoroquinolone antibiotic, on the cytochrome p450 enzymes in fish, although its use as antimicrobial agent in aquaculture has been put forward. therefore, the in vivo and in vitro effects of ef on hepatic p450 enzymes of sea bass, a widespread food-producing fish, have been evaluated. sea bass pretreated with a single dose of ef (3 mg/kg i.p.) or with three daily doses of ef (1 mg/kg i.p.) markedly depressed the microsomal n ... | 2003 | 12413791 |
| chemiluminescence response of beta-glucan stimulated leukocytes isolated from different tissues and peritoneal cavity of dicentrarchus labrax. | the respiratory burst of leukocytes isolated from sea bass (dicentrarchus labrax) pronephros, peritoneal cavity (p.c.), spleen and blood, was measured by a chemiluminescence (cl) assay after stimulation with beta-glucan. the cl response by p.c. and pronephros leukocytes was significantly higher than that expressed by a similar number of cells separated from spleen and blood. this probably reflects the observation that the proportion of macrophages and neutrophils was highest in the populations o ... | 2003 | 12711275 |
| peculiar gene organisation and incomplete splicing of sea bass (dicentrarchus labrax l) interleukin-1beta. | in this paper we describe the gene organisation of sea bass dicentrarchus labrax interleukin-1beta (il-1beta) and the presence of incompletely spliced forms of this gene. interestingly, the sea bass il-1beta gene comprises five exons and four introns, thus being remarkably different from other known teleost and mammalian genes. the sizes of the introns were typically much shorter than in mammals and this feature, together with the loss of two introns, gave a much smaller gene than in mammals (2. ... | 2003 | 12823998 |
| apoptosis of sea bass (dicentrarchus labrax l.) neutrophils and macrophages induced by experimental infection with photobacterium damselae subsp. piscicida. | the infection of sea bass (dicentrarchus labrax l.) by intraperitoneal (i.p.) injection of the agent of fish pasteurellosis photobacterium damselae subsp. piscicida resulted in the apoptosis of peritoneal neutrophils and macrophages. all the eight virulent and none of the two non-virulent strains tested exhibited apoptogenic activity. a secreted bacterial protein(s) is a likely candidate as the factor(s) responsible for this activity, since no apoptosis was induced by i.p. injected uv-killed vir ... | 2003 | 12834617 |
| virulence and molecular typing of vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and european sea bass. | vibrio harveyi was isolated from internal organs or ulcers of diseased and apparently healthy gilthead sea bream (sparus aurata) and european sea bass (dicentrarchus labrax) cultured in several fish farms located on the spanish mediterranean coast. the prevalence of the bacterium was significantly higher in european sea bass than in gilthead sea bream, and was closely related to the season in both fish species, occurring almost exclusively on warm months (june to november). after phenotypic char ... | 2003 | 12866856 |
| viral encephalopathy and retinopathy of dicentrarchus labrax (l.) farmed in fresh water in greece. | | 2003 | 12899411 |
| expression and characterization of european sea bass (dicentrarchus labrax) somatolactin: assessment of in vivo metabolic effects. | the complementary dna coding for european sea bass somatolactin was expressed in the pet-3a bacteria expression vector. the recombinant somatolactin (rbsl) was purified by size exclusion chromatography, and 95% of the protein remained in the oxidized form with negligible aggregation over prolonged cold storage. the identity of the recombinant protein was demonstrated by western blotting with a rabbit polyclonal antibody against gilthead sea bream somatolactin. the same antibody was utilized in a ... | 2003 | 12925923 |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| post-haemorrhagic anaemia in sea bass, dicentrarchus labrax (l.), caused by blood feeding of ceratothoa oestroides (isopoda: cymothoidae). | the effects of the fish parasitic isopod, ceratothoa oestroides (risso), on haematological parameters of its cage-cultured sea bass host, dicentrarchus labrax (l.), were studied. analyses of blood parameters (cell counts, haemoglobin content and haematocrit) were carried out on parasitized and unparasitized sea bass from a fish farm in turkey. parasitized fish had significantly lowered erythrocyte counts, haematocrit and haemoglobin values and significantly increased leucocyte counts. blood feed ... | 2003 | 12946009 |
| investigation of media formulations promoting differential antigen expression by photobacterium damsela ssp. piscicida and recognition by sea bass, dicentrarchus labrax (l.), immune sera. | photobacterium damsela ssp. piscicida (phdp) isolates were grown in various bacteriological media, in eukaryotic cell culture media and in the presence of fish cells (resembling some aspects of in vivo growth environments). bacterial cells, extracellular products (ecps) and crude capsular polysaccharide were isolated and analysed by electrophoresis and western blot using sea bass sera. growth in bacteriological media conserved the synthesis of cell and extracellular components when these were co ... | 2003 | 12962207 |
| a serum fucolectin isolated and characterized from sea bass dicentrarchus labrax. | a lectin specific for fucose and galactose was isolated by affinity chromatography on sepharose cl-6b from the serum of dicentrarchus labrax. the hemagglutinating activity against rabbit erythrocytes was calcium-independent, and reached its maximum at 37 degrees c. two protein components were found in the hemagglutinating fractions eluted from the sepharose column. only the 34 kda component (dll2) eluted from the polyacrylamide gels (sds-page) showed agglutinating activity against rabbit erythro ... | 2001 | 11687307 |
| the immune system of sea bass, dicentrarchus labrax, reared in aquaculture. | the sea bass dicentrarchus labrax is one the most important seawater fish species of south europe and mediterranean aquaculture, and studies on its immune system are important for both scientific and applied purposes. in this paper, we summarise the results obtained in studies of the immune system in this species, and present original data on cell-mediated acquired immune response. | 2002 | 11696380 |
| pcb and ddt contamination in cultivated and wild sea bass from ria de aveiro, portugal. | polychlorinated biphenyls (pcbs) and dichlorodiphenyltrichloroethane (ddt) and metabolites were quantified in muscle and liver of sea bass (dicentrarchus labrax) collected in ria de aveiro and in two fish farms. sea bass from natural environment showed lower levels than fish from farming, which may be partly attributed to the higher lipid content of cultivated fish. pcb congener distribution in tissues of sea bass from the two farms resembled that of diet pellets suggesting that commercial diet ... | 2004 | 14659952 |
| effects of photoperiod on pituitary levels of three forms of gnrh and reproductive hormones in the male european sea bass (dicentrarchus labrax, l.) during testicular differentiation and first testicular recrudescence. | photoperiod is one of the main external cues in the control of different stages of fish development. in the present work, the effect of several photoperiods, known to delay spawning in adult fish, was investigated in juvenile male sea bass (dicentrarchus labrax) during testicular differentiation and first testicular recrudescence. fish were exposed to constant long (lo), expanded (ex), constant short photoperiod with long photoperiod in october (sloct) and constant short photoperiod with long ph ... | 2004 | 14980795 |
| comparison of the api 20e and bbl crystal e/nf identification systems for differentiating bacterial isolates from apparently healthy reared sea bass (dicentrarchus labrax). | the ability of two commercial rapid identification systems, api 20e and bbl crystal e/nf, to reliably identify bacterial isolates from the internal organs of reared sea bass were compared. the tests gave different results: api 20e identified bacteria as pseudomonas spp. with 37% accuracy, while bbl crystal e/nf identified them as flavobacterium odoratum with 99% accuracy. although f. odoratum is not a marine fish pathogen, conventional tests conducted with the same isolates were more indicative ... | 2004 | 14992239 |
| modelling of fish interleukin-1 and its receptor. | this paper presents original data regarding: the three-dimensional modelling of rainbow trout (oncorhynchus mykiss) and sea bass (dicentrarchus labrax) il-1beta, the modelling of trout il-1 receptor type i (il-1ri) and the modelling of trout il-1beta bound to its receptor. the 3d models of trout and sea bass il-1beta molecules were predicted by comparison with those already available of human and mouse, and, in both cases, a structure consisting of a beta-trefoil fold was obtained, a motif well ... | 2004 | 15062642 |
| glutathione protects heavy metal-induced inhibition of hepatic microsomal ethoxyresorufin o-deethylase activity in dicentrarchus labrax l. | the in vitro effects of chromium (cr(vi)), copper (cu2+), iron (fe2+), mercury (hg2+), and zinc (zn2+) were assessed on liver microsomal ethoxyresorufin o-deethylase (erod) activity from a sea bass (dicentrarchus labrax l.) preexposed under laboratory conditions to 2.7 microm beta-naphthoflavone. the reduced glutathione (gsh) protection potential against heavy metal effects was also studied. the heavy metal concentration ranges used for this study were as follows: 10 pm-5 mm cr(vi), 10 pm-100 mi ... | 2004 | 15223263 |
| sphaerospora dicentrarchi and s. testicularis (myxozoa: sphaerosporidae) in farmed european seabass (dicentrarchus labrax) from italy. | the distribution of sphaerospora dicentrarchi sitjà-bobadilla et alvarez-pellitero, 1992 and s. testicularis sitjà-bobadilla et alvarez-pellitero, 1990, myxozoan parasites of european seabass dicentrarchus labrax (l.), was investigated in different farming systems in italy. in total, 1406 fish were examined. high s. dicentrarchi prevalence was observed in all the farming systems involved in this survey (extensive farms: 51.5%; intensive farms: inland 59.6%, inshore floating cages 76.2%, offshore ... | 2004 | 15357398 |
| new insights in developmental origins of different gnrh (gonadotrophin-releasing hormone) systems in perciform fish: an immunohistochemical study in the european sea bass (dicentrarchus labrax). | the knowledge of the roles and origins of different gonadotrophin-releasing hormone (gnrh) systems could greatly contribute to improve the understanding of mechanisms involved in the physiological control of early development, puberty and spawning. thus, in this study, we have analyzed the distribution of the cells expressing salmon gnrh, seabream gnrh and chicken gnrh-ii forms in the brain and pituitary of developing sea bass using specific antibodies to their corresponding gnrh-associated pept ... | 2004 | 15363486 |
| molecular cloning and characterisation of sea bass (dicentrarchus labrax l.) caspase-3 gene. | caspase-3 is one of the major caspases operating in apoptosis, cleaving and inactivating a number of molecules and largely contributing to the apoptotic phenotype and the dismantling of the apoptoting cell. the opening reading frame of sea bass (dicentrarchus labrax l.) caspase-3 has 281 amino acids. the complete sequence of caspase-3 shows a very close homology to the correspondent sequence from other vertebrates, in particularly with that of takifugu rubripes and oryzias latipes, with 87.7 and ... | 2007 | 16780952 |
| genomic classification of new betanodavirus isolates by phylogenetic analysis of the coat protein gene suggests a low host-fish species specificity. | viral encephalopathy and retinopathy is a devastating disease that causes neurological disorders and high mortality in a large number of cultivated marine fish species around the world. it is now established that several viral strains classified in the genus betanodavirus of the family nodaviridae are the aetiological agents of this disease. betanodaviruses can be classified into four genotypes based on the coat protein gene sequence. here, the coat protein genes of the three major strains isola ... | 2004 | 15448371 |
| effect of dietary iron overload on photobacterium damselae ssp. piscicida pathogenicity in sea bass, dicentrarchus labrax (l.). | | 2004 | 15509262 |
| production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-i in european sea bass (dicentrarchus labrax). | a specific radioimmunoassay (ria) for european sea bass (dicentrarchus labrax) growth hormone (gh) was developed and validated. for this purpose, a stable source of gh was produced by means of recombinant dna technology in a bacteria system. the identity of the purified protein (ion exchange chromatography) was demonstrated by western blot and a specific gh antiserum was raised in rabbit. in western blot and ria system, this antiserum recognized specifically native and recombinant gh, and it did ... | 2004 | 15560873 |
| parasitic diseases of marine fish: epidemiological and sanitary considerations. | over recent decades, parasitic diseases have been increasingly considered a sanitary and economic threat to mediterranean aquaculture. in order to monitor the distribution of parasites in cultured marine fish from italy and study their pathogenic effects on the host, a three-year survey based on parasitological and histopathological exams was carried out on 2141 subjects from eleven fish species and coming from different farming systems (extensive, intensive inland farms, inshore floating cages, ... | 2006 | 16881387 |
| short- and long-term effects of a dietary yeast beta-glucan (macrogard) and alginic acid (ergosan) preparation on immune response in sea bass (dicentrarchus labrax). | the present study investigated the immunomodulatory activity of ergosan, an algal extract containing alginic acid, and macrogard, a yeast extract containing beta-glucans, on innate and specific immunity in sea bass (dicentrarchus labrax). four cycles of experimental feeding using normal fish feed formulation (control group) supplemented with ergosan (0.5%) or macrogard (0.1%) were performed at 60-day intervals (15 days of treatment+45 days of suspension). serum complement, lysozyme, total protei ... | 2005 | 15561561 |
| sea bass (dicentrarchus labrax) sex hormone binding globulin: molecular and biochemical properties and phylogenetic comparison of its orthologues in multiple fish species. | sex hormone binding globulin (shbg) binds and transports androgens and estrogens in the blood of vertebrate species including fish. we have used oligonucleotide primers corresponding to highly conserved regions of the shbg coding sequences within the zebrafish and fugufish genomes to obtain a 1528 bp cdna encoding shbg from tissue rna extracts from the european sea bass. amino-terminal sequence analysis of recombinant sea bass shbg indicated that its deduced precursor polypeptide includes a 35-r ... | 2005 | 15607525 |