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cypress (cupressus sempervirens) pollen allergens: identification by protein blotting and improved detection of specific ige antibodies.on the basis of results of an investigation of the effects of different treatments employed, a dialysed and reduced extract of cupressus sempervirens was separated electrophoretically on sodium dodecylsulphate-polyacrylamide gels before being transferred and then fixed with glutaraldehyde to nitrocellulose membrane. probing with sera from 91 subjects allergic to c. sempervirens pollen followed by detection of bound ige antibodies with [125i]-labelled anti-human ige revealed 17 ige-binding protei ...19911937919
cross-reactivity between the pollens of cupressus sempervirens (common cypress) and of cryptomeria japonica (japanese cedar).a clinical and immunologic study was performed comparing a group of french patients allergic to the pollens of cypress (cupressus sempervirens, which belongs to the cupressaceae family) and a group of japanese patients allergic to the pollens of sugi (cryptomeria japonica, which belongs to the taxodiaceae family). by skin testing, rast, and rast inhibition, clear cross-reactivity was detected between the two pollens. no cross-reactivity was detected between the pollens of cupressus sempervirens ...19863729079
[catechins and flavanolic oligomers from cupressus sempervirens l. comparative in vitro elastase inhibitory and in vivo angioprotective activity]. 19846567463
cypress pollen allergy. identification of allergens and crossreactivity between divergent species.studies employing sera from 34 subjects allergic to white cypress pine (callitris glaucophylla) pollen identified 18 ige antibody-binding components in the pollen of this species, five of which (mws approximately 94, 68, 64, 43 and 34 kda) were recognized by all of the sera. protein blotting and quantitative inhibition studies revealed clear cross-reactivity between c. glaucophylla and cupressus sempervirens pollen proteins and striking similarities in the ige recognition band patterns of the tw ...19947522944
epitopes on cry j i and cry j ii for the human ige antibodies cross-reactive between cupressus sempervirens and cryptomeria japonica pollen.forty sea from french patients allergic to cupressus sempervirens pollen were tested for cross-reactivities against cry j i, cry j ii (major allergens of cryptomeria japonica pollen) and other pollen allergens from botanically related plants. seventy-three per cent of the sera reacted with either cry j i or cry j ii, or with both of them. these ige cross-reactions were blocked effectively by mab 046 (anti-cry j i) or n26, t27 (anti-cry j ii), and weakly by mabs 052, 027 and 026 (anti-cry j i). f ...19937679186
prevalence of patent and latent atopy among a general normal adult population in the south east of france by rast investigation and correlation with circulating total ige levels.a population of 512 adults (men: 266, 52%, women: 246, 48%) with mean age 41.4 years (s.d. 10.7) was investigated. this population was subdivided into five groups: marseilles city, manosque a small town in a prealpine area, the area surrounding the etang de berre, a rural area in northern bouches du rhône and chateaurenard, a small town in an intensive agricultural area. rasts to a panel of eight allergens (lolium perenne, parietaria officinalis, cupressus sempervirens, olea europea, cat dander, ...19938160568
airborne cupressaceae pollen in southern italy.the allergenic airborne pollen of cupressaceae was surveyed in the area of bari (southern italy) over an 8-year period (1982-1989). a burkard 7-day recording volumetric spore trap was used for the aerobiologic investigation. the results showed a notable annual pollinic presence of airborne cupressaceae pollen, the largest amount recorded in winter and early spring. remarkable fluctuations of the cupressaceae pollen counts in a 2-year cycle were also observed. the features of the main pollen seas ...19938328713
cross-reactivity between cupressus arizonica and cupressus sempervirens pollen extracts.cupressus arizonica and c. sempervirens, two species belonging to the cupressaceae family, are recognized as an important cause of respiratory allergies in countries with a mediterranean climate.19968876556
sapinofuranones a and b, two new 2(3h)-dihydrofuranones produced by sphaeropsis sapinea, a common pathogen of coniferstwo new 5-substituted dihydrofuranones, named sapinofuranones a and b (1 and 2), were isolated from liquid cultures of sphaeropsis sapinea, a phytopathogenic fungus causing a wide range of disease symptoms on conifers. a fungal strain isolated from cupressus macrocarpa produced both 1 and 2 at concentrations higher than those produced by the strain isolated from cupressus sempervirens. bioassay of 1 and 2 gave epinasty and brown discoloration on petioles of tomato leaves, sapwood stain on inner ...199910075753
two-dimensional electrophoretic analysis of proteins associated with somatic embryogenesis development in cupressus sempervirens l.two-dimensional gel electrophoretic analysis and histological studies were performed on somatic embryos in cypress. embryogenic cultures were obtained from in vitro culture of immature seeds. on a modified murashige and skoog (ms) medium they showed an intense and repetitive cleavage polyembryogenesis phenomenon which maintained them in a continuous proliferating status instead of undergoing a complete embryogenic development. only the addition of bovine serum albumin to the culture allowed soma ...199910344292
efficient transmission of an introduced pathogen via an ancient insect-fungus associationin cupressus sempervirens the association between seed insects and tree pathogens has resulted in optimal exploitation of the cones. a fungus-infected cone can be inhabited by the nymphs of a true seed bug (orsillus maculatus), the adults of which may carry a heavy spore load at emergence. cones are infected when eggs are laid within the cone, most frequently via the emergence holes of a seed wasp (megastigmus wachtli). this symbiotic association evolved with the nonaggressive fungus pestalotiop ...199910541657
prevalence of sensitization to cupressus sempervirens: a 4-year retrospective study.in the last few years cupressus sempervirens has been identified as the cause of an increasing number of cases of late winter-early spring pollinosis in mediterranean countries. we conducted a 4-year retrospective study of a large group of subjects with documented allergic respiratory disease in order to determine the prevalence, clinical significance and annual rate of sensitization to c. sempervirens pollen. anamnestic data and skin prick tests (spt) with common aeroallergens and c. sempervire ...200111327403
sphaeropsidins d and e, two other pimarane diterpenes, produced in vitro by the plant pathogenic fungus sphaeropsis sapinea f. sp. cupressi.two pimarane diterpenes structurally related to sphaeropsidins were isolated from the liquid culture of sphaeropsis sapinea f. sp. cupressi, a plant pathogenic fungus causing a form of canker disease of italian cypress (cupressus sempervirens l.). the two metabolites, characterised by spectroscopic methods, were named sphaeropsidins d (0.40 mg l(-1)) and e (0.16 mg l(-1)). the same fungus produced sphaeropsidins a, b and c, sphaeropsidone and episphaeropsidone, which proved to be phytotoxic to c ...200211937160
damping-off in conifer seedling nurseries in noshahr and kelardasht.to study the damping-off of conifer seedlings, we have collected samples from the roots of conifer seedlings (pinus nigra, picea excelsa, abieces spp, cupressus arizonica, cupressus sempervirens) from nurseries in the south of iran (noshahr and kelardasht). after disinfecting the samples, we have used standard media like pda, ma and cla. the following fungi were identified: fusarium solani, fusarium oxysporum, fusarium sambucinum, clamydosporium, rhizoctonia solani, cylindrocarpon spp., alternar ...200112425024
susceptibility of cypress seedlings to the eriophyoid mite trisetacus juniperinus.in italian nurseries and young groves of evergreen cypress (cupressus sempervirens l.), the eriophyoid mite trisetacus juniperinus (nal.) is considered a very serious pest. a rating system of damage symptoms was developed to investigate the susceptibility of different cypress seedling families to the mite. based on this system, the seedlings were evaluated for three years in the nursery and in two field locations after transplanting. data obtained in the nursery allowed the cypress families to b ...200212537293
effects of cupressus sempervirens cone extract on lipid parameters in wistar rats.lipid-lowering drugs have been developed to treat hyperlipidemia, but they are expensive, while serious side-effects may occasionally occur. in albania, a hydroalcoholic extract of the cones of cupressus sempervirens is used to treat a variety of disorders, including hyperlipidemia. the purpose of our study was to assess the effects of cupressus sempervirens cone extract (cse) on the lipid profile of wistar rats.200312655800
an epidemiological survey of cupressaceae pollenosis in italy.allergic reaction to cupressaceae and taxodiaceae pollens appears to be on the increase in the last years in italy. an epidemiological survey on this pollenosis was conducted in 12 italian centers. the diagnostic panel consisted of seven cupressaceae and taxodiaceae genus (cupressus sempervirens, cupressus arizonica, cryptomeria japonica, chamaecyparis obtusa, thuja orientalis, taxus baccata, and juniperus oxycedrus). a total of 3057 pollen-sensitized outpatients were examined. the study took pl ...200212940232
asthma induced by canary food mix.a 42-year-old woman reported immediate rhinoconjunctivitis, asthma, and contact urticaria while handling bird food. skin-prick tests were positive to lolium, cynodon, phragmites, cupressus sempervirens, cupressus arizonica, chenopodium, sunflower pollen and seed, mugwort, chamomile, chrysanthemum, taraxacum, canary seed, and black seed (guizotia abyssinica). the patient's serum-specific immunoglobulin (ige) to taraxacum, black seed, and canary seed was positive. enzyme-linked immunosorbent assay ...200312974193
use of pruning waste compost as a component in soilless growing media.the objective of this work was to study the use of pruning wastes compost (pwc) as a growing media component for ornamental plants. the main physical, chemical and biological characteristics of pwc were analysed in order to evaluate its suitability for use in soil-less cultivation. six growth substrates were prepared by mixing pwc with peat (p), ground leaves (gl), sand (s) and spent mushroom compost (smc) in different proportions. two different pot experiments were carried out to test its chara ...200515501667
allergy to cypress pollen.although cupressus sempervirens has been spread over southern europe since antiquity, cypress pollen allergy has not been reported until 1945. in france, the very first case reports were published in 1962. since then, the prevalence of cypress pollinosis seems to demonstrate an upward trend, concomitantly with the increased use of cypress trees as ornamental plants, as wind breaks and as hedges. hyposensitization, using improved pollen extracts, is increasingly prescribed. besides, prevention me ...200515679713
in vitro antimycotic activity of some plant extracts towards yeast and yeast-like strains.as part of screening aimed at the selection of novel antimycotic compounds of vegetable origin, leaf extracts of camellia sinensis l., cupressus sempervirens l. and pistacia lentiscus l. and the seed extract of glycine soja sieb. et zucc. were tested against yeast and yeast-like species implicated in human mycoses. of the extracts only those of c. sinensis (obtained from a commercial preparation of green tea) exhibited broad activity towards candida glabrata, clavispora lusitatiae, cryptococcus ...200515798996
comparative screening of plant essential oils: phenylpropanoid moiety as basic core for antiplatelet activity.essential oils extracted from different plants (anthemis nobilis l., artemisia dracunculus l., cannabis sativa l., cupressus sempervirens l., cymbopogon citratus (dc.) stapf., curcuma longa l., foeniculum vulgare l., hypericum perforatum l., hyssopus officinalis l., mentha spicata l., monarda didyma l., ocimum basilicum l., ocotea quixos kosterm., origanum vulgare l., pinus nigra j.f. arnold, pinus silvestris l., piper crassinervium kunth., rosmarinus officinalis l., salvia officinalis l., salvi ...200616274702
enzymes of glyoxylate in conifers.the high level of lipids in seeds of some species of conifers suggested that the glyoxylate cycle might have a role in conifer seed metabolism.six species (pinus pinea, pinus pinaster, pinus canariensis, pinus strobus, abies alba, and cupressus sempervirens) were investigated for their lipid content and malate synthase and isocitrate lyase level. the fatty acid composition of the triglyceride fraction was also investigated. the correlation between lipid content of germinating seed with the prese ...196816656892
chemical and biological characterisation of sapinopyridione, a phytotoxic 3,3,6-trisubstituted-2,4-pyridione produced by sphaeropsis sapinea, a toxigenic pathogen of native and exotic conifers, and its derivatives.a phytotoxic trisubstituted 2,4-pyridione, named sapinopyridione, was isolated from the culture filtrates of sphaeropsis sapinea, a fungal pathogen of conifers occurring world-wide. three strains were isolated from two cypress species. strain d-55 isolated from cupressus sempervirens resulted high producer of sapinopyridione (12.3 mg l(-1)), whereas strain d-54 isolated from the same cypress species was low producer (1.1 mg l(-1)); strain d-50 isolated from c. macrocarpa was intermediate produce ...200616712886
ritual plants of muslim graveyards in northern israel.this article surveys the botanical composition of 40 muslim graveyards in northern israel, accompanied by an ethnobotanical study of the folkloristic traditions of the use of these plants in cemeteries. three groups of plants were found to be repeated systematically and were also recognized for their ritual importance: aromatics herbs (especially salvia fruticosa and rosmarinus officinalis), white flowered plants (mainly narcissus tazetta, urginea maritima, iris spp. and pancratium spp.) and cup ...200616961931
identification of italian cypress (cupressus sempervirens) pollen allergen cup s 3 using homology and cross-reactivity.the prevalence of seasonal allergic diseases of the upper airways is increasing in industrialized countries. the cupressaceae are important causes of pollinosis, particularly in europe.200617042139
phytochemical investigation and hepatoprotective activity of cupressus sempervirens l. leaves growing in egypt.three phenolic compounds cosmosiin, caffeic acid, and p-coumaric acid were isolated for the first time from the leaves of cupressus sempervirens l., together with cupressuflavone, amentoflavone, rutin, quercitrin, quercetin, myricitrin. the isolated compounds were identified using (1)h- and (13)c-nmr spectra. the hepatoprotective activity of the meoh extract was carried out in liver homogenate of normal and ccl(4)-treated rats; a significant decrease in glutamate oxaloacetate transaminase, gluta ...200717680494
phytochemical analysis and in vitro antiviral activities of the essential oils of seven lebanon species.the chemical composition of the essential oils of laurus nobilis, juniperus oxycedrus ssp. oxycedrus, thuja orientalis, cupressus sempervirens ssp. pyramidalis, pistacia palaestina, salvia officinalis, and satureja thymbra was determined by gc/ms analysis. essential oils have been evaluated for their inhibitory activity against sars-cov and hsv-1 replication in vitro by visually scoring of the virus-induced cytopathogenic effect post-infection. l. nobilis oil exerted an interesting activity agai ...200818357554
prevalence of cypress pollen sensitization and its clinical importance in izmir, turkey, with cypress allergy assessed by nasal provocation.pollens from the cupressaceae family are considered important allergens in the mediterranean area, though reports of the prevalence of allergic symptoms have ranged from 1.04% to 35.4%. our aim was to detect the prevalence of cypress pollen sensitization and determine its clinical importance in patients with seasonal respiratory allergy.200818361102
in vitro antimicrobial activity and antagonistic effect of essential oils from plant species.kahramanmaras, is a developing city located in the southern part of turkey thymus eigii (m. zohary and rh. davis) jalas, pinus nigraam. sub sp pallasiana and cupressus sempervirens l. are the useful plants of the kahramanmaras province and have been understudy since 2004 for the traditional uses of plants empiric drug, spice, herbal tea industry herbal gum and fuel. the study was designed to examine the antimicrobial activities of essential oils of these plants by the disc diffusion and minimum ...200718380074
the cypress aphids in the south of france.in the south of france, cinara (cupressobium) cupressi, the main cypress aphid, has been studied during 6 years on a mixed hedgerow in which cupressus sempervirens and c. arizonica had been planted alternatively. in the area, this monoecious aphid is anholcyclic and heavily attended by ants. continuous observation of the trees and of the colonies allowed a description of the population dynamics and the characterization of the damages. the two cypress species are regularly attacked, but the aphid ...200718399464
antiproliferative effects of essential oils and their major constituents in human renal adenocarcinoma and amelanotic melanoma cells.the purpose of this study was to evaluate cytotoxic activity of platycladus orientalis, prangos asperula and cupressus sempervirens ssp. pyramidalis essential oils and to identify active components involved in inhibition of population growth of human cancer cell lines.200819040575
growth and survival response of potted cupressus sempervirens seedlings to different soils.in february 2001, one-year bareroot cypress (cupressus sempervirens var. horizontalis) seedlings were replanted in plastic pot in a lowland nursery located in southern coast of the caspian sea (north of iran). soils of pots consisted of 1:1 sand:clay (a), pure sand (b), 2:1 sand:clay (c), 1:1:1 sand:clay:organic matter (d), 1:1:2 sand:clay:organic matter (e). in each soil treatment a high value of survival and growth was appeared in july and progressively decreased till november. in each month t ...200719069934
is cupressus sempervirens native in italy? an answer from genetic and palaeobotanical data.this study represents the first large-scale analysis using nuclear molecular markers to assess genetic diversity and structure of cupressus sempervirens l.. genetic and fossil data were combined to infer the possible role of human activity and evolutionary history in shaping the diversity of cypress populations. we analysed 30 populations with six polymorphic nuclear microsatellite markers. dramatic reductions in heterozygosity and allelic richness were observed from east to west across the spec ...200919389173
evaluation of the in vitro anticancer, antimicrobial and antioxidant activities of some yemeni plants used in folk medicine.the present research study deals with the evaluation of sixty four methanolic and aqueous extracts of thirty yemeni plants used in traditional medicine for their in vitro antiproliferative activity against three human cancer cell lines in a microtiter plate assay based on cellular staining with crystal violet, for their antimicrobial activity against antibiotic susceptible three gram-positive, three gram-negative bacterial and one fungal stains and three multiresistant staphylococcus strains by ...200919435146
allergenicity and cross-reactivity of pine pollen.pine pollen has long been considered a non-allergenic pollen. the large size of the grain and its low levels of proteins are the main reasons invoked to explain this low allergenicity. the aim of this study was to describe the main allergenic bands of pinus radiata (pr) and its cross-reactivity with other pine species, other conifers and grass pollen.200919573163
8,8''-biapigeninyl stimulates osteoblast functions and inhibits osteoclast and adipocyte functions: osteoprotective action of 8,8''-biapigeninyl in ovariectomized mice.8,8''-biapigeninyl (ba), a condensation product of two apigenin molecules, is found abundantly in the nuts of cupressus sempervirens. we investigated the effects of ba on murine bone cells in vitro and in ovariectomized (ovx) mice. ba at 10(-10)m and 10(-8)m, inhibited osteoclastogenesis of bone marrow cells (bmcs) and displayed concentration dependence. ba at 10(-8) m and 10(-6) m inhibited differentiation of 3t3-l1 and bmcs to mature adipocytes. ba (10(-10)m) stimulated osteoblast proliferatio ...201020380869
biomonitoring of chemical elements in an urban environment using arboreal and bush plant species.the aim of this work was to investigate the possibility of using several bush and arboreal plant species, usually present as ornamental plants in street and parks, as environmental indicators of pollution. this is a research paper that evaluates the real possibility of using a fast and low-cost procedure to evaluate the pollution degree through data obtained from plant species growing within an urban environment.201120559747
biodegradable gelatin-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation.essential oils of clove (syzygium aromaticum l.), fennel (foeniculum vulgare miller), cypress (cupressus sempervirens l.), lavender (lavandula angustifolia), thyme (thymus vulgaris l.), herb-of-the-cross (verbena officinalis l.), pine (pinus sylvestris) and rosemary (rosmarinus officinalis) were tested for their antimicrobial activity on 18 genera of bacteria, which included some important food pathogen and spoilage bacteria. clove essential oil showed the highest inhibitory effect, followed by ...201020688230
rhizosphere dynamics during phytoremediation of olive mill wastewater.the potential of phytoremediation as a treatment option for olive mill wastewater (omw) was tested on five perennial tree species. cupressus sempervirens and quercus ilex proved tolerant to six-month omw treatment followed by six-month water irrigation, whereas salix sp. and laurus nobilis and, later, pinus mugo suffered from phytotoxic effects. test plants were compared to controls after treatment and irrigation, by monitoring biochemical and microbiological variations in the rhizosphere soil. ...201021256739
pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides.to cite this article: vinhas r, cortes l, cardoso i, mendes vm, manadas b, todo-bom a, pires e, veríssimo p. pollen proteases compromise the airway epithelial barrier through degradation of transmembrane adhesion proteins and lung bioactive peptides. allergy 2011; doi: 10.1111/j.1398-9995.2011.02598.x. abstract: background:  allergic disorders, such as seasonal rhinitis and asthma, are increasing causes of morbidity worldwide and often result from exposure to airborne pollen. pollen allergy has ...201121480927
protective role of juniperus phoenicea and cupressus sempervirens against ccl(4).to investigate the role of cupressus sempervirens (c. sempervirens) and juniperus phoenicea (j. phoenicea) extracts as therapeutic effect against ccl(4) with biochemical, histopathological evaluations.201021577307
mechanical stability of trees under dynamic loads.tree stability in windstorms and tree failure are important issues in urban areas where there can be risks of damage to people and property and in forests where wind damage causes economic loss. current methods of managing trees, including pruning and assessment of mechanical strength, are mainly based on visual assessment or the experience of people such as trained arborists. only limited data are available to assess tree strength and stability in winds, and most recent methods have used a stat ...200621642099
antioxidant and antiacetylcholinesterase activities of some commercial essential oils and their major compounds.the commercial essential oils of citrus aurantium l., cupressus sempervirens l., eucalyptus globulus labill., foeniculum vulgare mill. and thymus vulgaris l., isolated by steam distillation by a company of morocco were evaluated in terms of in vitro antioxidant activity through several methods. in vitro acetylcholinesterase inhibitory activity was also determined. citrus limon (l.) burm. f. oil was also studied, but it was obtained by peel expression. the best antioxidant was t. vulgaris oil, in ...201121900869
structural characterization of the o-chain polysaccharide from an environmentally beneficial bacterium pseudomonas chlororaphis subsp. aureofaciens strain m71.pseudomonas chlororaphis subsp. aureofaciens strain m71 was isolated from the root of a tomato plant and it was able to control in vivo fusarium oxysporum f. sp. radicis-lycopersici responsible for the tomato crown and root rot. recently, strain m71 was evaluated even for its efficacy in controlling seiridium cardinale, the causal agent of bark canker of common cypress (cupressus sempervirens l.). strain m71 ability to persist on the tomato rhizosphere and on the aerial part of cypress plants co ...201122036123
terpene arms race in the seiridium cardinale - cupressus sempervirens pathosystem.the canker-causing fungus seiridium cardinale is the major threat to cupressus sempervirens worldwide. we investigated the production of terpenes by canker-resistant and susceptible cypresses inoculated with s. cardinale, the effect of these terpenes on fungal growth, and the defensive biotransformation of the terpenes conducted by the fungus. all infected trees produced de novo terpenes and strongly induced terpenic responses, but the responses were stronger in the canker-resistant than the sus ...201626796122
ephedra alte (joint pine): an invasive, problematic weedy species in forestry and fruit tree orchards in jordan.a field survey was carried out to record plant species climbed by ephedra alte in certain parts of jordan during 2008-2010. forty species of shrubs, ornamental, fruit, and forest trees belonging to 24 plant families suffered from the climbing habit of e. alte. growth of host plants was adversely affected by e. alte growth that extended over their vegetation. in addition to its possible competition for water and nutrients, the extensive growth it forms over host species prevents photosynthesis, s ...201222645486
antiproliferative, antifungal, and antibacterial activities of endophytic alternaria species from cupressaceae.recent research has shown the bioprospecting of endophytic fungi from cupressaceae. here, we further uncover that the healthy cypress plants such as cupressus arizonica, cupressus sempervirens var. cereiformis, and thuja orientalis host highly bioactive endophytic alternaria fungal species. indeed, endophytic alternaria alternata, alternaria pellucida, and alternaria tangelonis were recovered from healthy cupressaceous trees. biodiversity and bioactivity of recovered endophytic alternaria specie ...201424801337
the skin prick test - european standards.skin prick testing is an essential test procedure to confirm sensitization in ige-mediated allergic disease in subjects with rhinoconjunctivitis, asthma, urticaria, anapylaxis, atopic eczema and food and drug allergy. this manuscript reviews the available evidence including medline and embase searches, abstracts of international allergy meetings and position papers from the world allergy literature. the recommended method of prick testing includes the appropriate use of specific allergen extract ...201323369181
diverse and bioactive endophytic aspergilli inhabit cupressaceae plant family.aspergilli are filamentous, cosmopolitan and ubiquitous fungi which have significant impact on human, animal and plant welfare worldwide. due to their extraordinary metabolic diversity, aspergillus species are used in biotechnology for the production of a vast array of biomolecules. however, little is known about aspergillus species that are able to adapt an endophytic lifestyle in cupressaceae plant family and are capable of producing cytotoxic, antifungal and antibacterial metabolites. in this ...201424912659
in-vitro antimicrobial activities of some iranian conifers.male and female leaves and fruits of eleven different taxons of iranian conifers (cupressus sempervirens var. horizontalis, c. sempervirens var. sempervirens, c. sempervirens cv. cereifeormis, juniperus communis subsp. hemisphaerica, j. excelsa subsp. excelsa, j. excelsa subsp. polycarpos, j. foetidissima, j. oblonga, j. sabina, platycladus orientalis and taxus baccata) were collected from different localities of iran, dried and extracted with methanol. the extracts were tested for their antimic ...201324250573
chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the mediterranean cypress (cupressus sempervirens l.).cupressus sempervirens is a medicinal plant traditional, its dried leaves are used in treatment of stomach pain, diabetes, inflammation, toothache, laryngitis and as contraceptive.201424890383
in vitro antiplasmodial, antileishmanial and antitrypanosomal activities of selected medicinal plants used in the traditional arabian peninsular region.worldwide particularly in developing countries, a large proportion of the population is at risk for tropical parasitic diseases. several medicinal plants are still used traditionally against protozoal infections in yemen and saudi arabia. thus the present study investigated the in vitro antiprotozoal activity of twenty-five plants collected from the arabian peninsula.201222520595
fungal endophyte diversity and bioactivity in the mediterranean cypress cupressus sempervirens.fungal endophytes were isolated from the mediterranean cypress cupressus sempervirens. eleven taxa of fungi, all within the ascomycota, were identified based on pcr amplification and sequencing of the internal transcribed spacer sequences of nuclear ribosomal dna (its rdna) with taxonomic identity assigned using the ncbi nucleotide megablast search tool. the endophytic fungi included alternaria multiformis, didymella sp., phoma sp., phoma herbarum, pyrenochaeta sp. (dothideomycetes), penicillium ...201525527365
essential oils composition and antimicrobial activity of six conifers harvested in lebanon.the chemical composition and antimicrobial activity of the essential oils (eos) of six conifers harvested in lebanon, abies cilicica, cupressus sempervirens, juniperus excelsa, juniperus oxycedrus, cedrus libani and cupressus macrocarpa gold crest, were investigated. the eos were obtained by hydrodistillation using a clevenger-type apparatus and characterized by gc and gc/ms analyses. a principal components analysis based on pearson correlation between essential oils chemical analyses was also c ...201727685246
planting sentinel european trees in eastern asia as a novel method to identify potential insect pest invaders.quarantine measures to prevent insect invasions tend to focus on well-known pests but a large proportion of the recent invaders were not known to cause significant damage in their native range, or were not even known to science before their introduction. a novel method is proposed to detect new potential pests of woody plants in their region of origin before they are introduced to a new continent. since asia is currently considered to be the main supplier of insect invaders to europe, sentinel t ...201525993342
preparation and characterisation of biodegradable pollen-chitosan microcapsules and its application in heavy metal removal.biosorbents have been widely used in heavy metal removal. new resources should be exploited to develop more efficient biosorbents. this study reports the preparation of three novel chitosan microcapsules from pollens of three common, wind-pollinated plants (acer negundo, cupressus sempervirens and populus nigra). the microcapsules were characterized (fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and elemental analysis) and used in removal of he ...201525479387
essential oil composition, adult repellency and larvicidal activity of eight cupressaceae species from greece against aedes albopictus (diptera: culicidae).the present study evaluated leaf essential oils from eight cupresaceae species; cupressus arizonica, cupressus benthamii, cupressus macrocarpa, cupressus sempervirens, cupressus torulosa, chamaecyparis lawsoniana, juniperus phoenicea, and tetraclinis articulata for their larvicidal and repellent properties against aedes albopictus, a mosquito of great ecological and medical importance. based on the lc(50) values, c. benthamii essential oil was the most active (lc(50) = 37.5 mg/l) while the other ...201323263252
identification of the plant origin of the botanical biomarkers of mediterranean type propolis.propolis is a honeybee product which bees produce by collecting resins from various botanical sources. the chemical composition of propolis is directly dependant on the availability of resinous plant materials in different geographic regions. this study was undertaken to evaluate the resinous plant sources used by bees to produce mediterranean type propolis. although this propolis type has already been the subject of numerous studies, its major botanical source had not yet been identified. in th ...201222799077
identification of low temperature stress regulated transcript sequences and gene families in italian cypress.cold acclimation is a complex transcriptionally controlled process regulated by many different genes and genic-interactions in plants. the northward spreading of woody species is mainly limited by winter harshness. to increase our knowledge about the biological processes underlying cold acclimation, plants evolved in warmer climates can serve as models. in this work, a suppression subtractive hybridization approach using pcr-select was used to isolate italian cypress (cupressus sempervirens l.) ...201525534982
anti-oxidant, anti-inflammatory and anti-proliferative activities of moroccan commercial essential oils.essential oils (eo) possess antimicrobial, anti-inflammatory, insect repellent, anti-cancer, and antioxidant properties, among others. in the present work, the antioxidant, anti-inflammatory and anti-proliferative activities of moroccan commercial eos (citrus aurantium, c. limon, cupressus sempervirens, eucalyptus globulus, foeniculum vulgare and thymus vulgaris) were evaluated and compared with their main constituents. t. vulgaris eo showed the best free radicals scavenging capacity. this eo wa ...201424868891
differential ige sensitization to cypress pollen associated to a basic allergen of 14 kda.the common cypress (cupressus sempervirens) (cups) pollen represents the first cause of respiratory allergies in the mediterranean basin. the aim of this study was to characterize a novel 14-kda cypress pollen allergen (bp14) allowing a clear dissociation of ige sensitization patterns among allergic patients. the biochemical and immunochemical characterization of bp14 included determination of its isoelectric point, molecular mass, extraction kinetics, ige binding prevalence, the presence of bro ...201222340670
double-blind placebo controlled specific immunotherapy with mixed cupressaceae taxodiaceae pollens in respiratory allergy to cupressus sempervirens.twenty adult patients presenting a respiratory allergy to the pollen of cupressus sempervirens were randomly divided into two groups; one received a placebo the other one an equal mixture of cupresssus sempervirens and cryptomeria japonica pollen during a period of 3 years (1993-1996). according to the following criteria: symptoms score, drug consumption, skin test and rast, nasal provocation test, the results for the treated group were statistically better than those for the placebo group. no s ...20049111873
cypress pollinosis: from tree to clinic.cypress (cupressus sp.pl) is a genus within the cupressaceae family. this family covers all of the earth's continents except for antarctica, and it includes about 160 species. the most important taxa for allergic diseases belong to five different genera: cupressus, hesperocyparis, juniperus, cryptomeria, and chamaecyparis. cupressaceae species share a common pollen type that can even include the genus taxus (taxaceae) when this plant is also present. as juniperus oxycedrus pollinates in october, ...201728401436
[analysis of the effectiveness of the spa and health resort-based treatment of the patients presenting with broncho-pulmonary pathology at the southern coast of the crimea depending on the period of flowering of mediterranean cypress (cupressus sempervirens)].the objective of the present study was to evaluate the influence of pollen of mediterranean cypress (cupressus sempervirens) on the effectiveness of the spa and health resort-based treatment (shrt) of the patients with broncho-pulmonary pathology at the southern coast of the crimea (scc).201727500676
lead removal from aqueous solutions by raw sawdust and magnesium pretreated biochar: experimental investigations and numerical modelling.lead removal from aqueous solutions by raw cypress (cupressus sempervirens l.) sawdust (rcs) and its derivative magnesium pretreated biochar (mg-b) was investigated under static and dynamic conditions through batch and column assays. the hydrus-1d model was used to estimate the transport parameters of the lead measured breakthrough curves. the batch experiments results showed that mg-b was very efficient in removing lead compared to rcs and several other previously tested natural and modified ma ...201627266649
possible land management uses of common cypress to reduce wildfire initiation risk: a laboratory study.accurate determination of flammability is required in order to improve knowledge about vegetation fire risk. study of the flammability of different plant species is essential for the mediterranean area, where most ecosystems are adapted to natural fire but vulnerable to recurrent human-induced fires, which are the main cause of forest degradation. however, the methods used to evaluate vegetation flammability have not yet been standardized. cupressus sempervirens is a native or naturalized forest ...201526046989
in vitro cholinesterase inhibitory and antioxidant effect of selected coniferous tree species.to explore cholinesterase inhibitory and antioxidant effect of six coniferous trees (abies bornmulleriana, picea pungens, juniperus communis, cedrus libani, taxus baccata, and cupressus sempervirens var. horizantalis).201525975497
strong induction of minor terpenes in italian cypress, cupressus sempervirens, in response to infection by the fungus seiridium cardinale.seiridium cardinale, the main fungal pathogen responsible for cypress bark canker, is the largest threat to cypresses worldwide. the terpene response of canker-resistant clones of italian cypress, cupressus sempervirens, to two differently aggressive isolates of s. cardinale was studied. phloem terpene concentrations, foliar terpene concentrations, as well as foliar terpene emission rates were analyzed 1, 10, 30, and 90 days after artificial inoculation with fungal isolates. the phloem surroundi ...201525740205
determination of epigenetic inheritance, genetic inheritance, and estimation of genome dna methylation in a full-sib family of cupressus sempervirens l.genetic inheritance and epigenetic inheritance are significant determinants of plant evolution, adaptation and plasticity. we studied inheritance of restriction site polymorphisms by the f-aflp method and epigenetic dna cytosine methylation inheritance by the f-msap technique. the study involved parents and 190 progeny of a cupressus sempervirens l. full-sib family. results from aflp genetic data revealed that 71.8% of the fragments studied are under mendelian genetic control, whereas faithful m ...201525726917
protective effect of cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats.cupressus sempervirens (c. sempervirens) belongs to the family cupressaceae. it is widspread in northern africa, greece, turkey, north america, cyprus and syria. cupressuflavone is the major ingredient of the plant leave extract. the aim of the present study was to evaluate the antiulcerogenic activity of the extract of c. sempervirens leaves in gastric ulcer tissues induced by indomethacin. the results of the present study revealed that indomethacin significantly decreased glutathione s-transfe ...201527486357
the effect of long-term wastewater irrigation on accumulation and transfer of heavy metals in cupressus sempervirens leaves and adjacent soils.wastewater reuse for agriculture is an important management strategy in areas with limited freshwater resources, yielding potential economic and environmental benefits. here the effects of long-term irrigation with wastewater on the nutrient contents of green and senesced leaves of cupressus sempervirens l. were assessed for three planted forests in egypt. stoichiometric ratios, transfer factors for nutrients from soil to plant and enrichment factors in contaminated soils were estimated and comp ...201525613764
osteogenic activity of natural diterpenoids isolated from cupressus sempervirens fruits in calvarial derived osteoblast cells via differentiation and mineralization.the aim of the present study was to investigate the antiosteoporotic activity of four structurally related diterpenoids: sugiol (1), trans-communic acid (2), 15-acetoxy imbricatolic acid (3) and imbricatolic acid (4). their osteogenic effect was evaluated by using validated models including alkaline phosphatase (alp) assay, mineralization assay and expression of osteogenic genes-bone morphogenetic protein-2 (bmp-2) and osteoblast transcription factor (runx2) - in primary calvarial cultures harve ...201425481392
nitrate uptake improvement by modified activated carbons developed from two species of pine cones.activated carbons from two species of pine cones (pinus canariensis and cupressus sempervirens) were prepared by phosphoric acid activation and tested for the removal of nitrate ions from aqueous solution. to investigate the feasibility of improving their nitrate adsorption capacity, two different post-treatments—a thermal treatment and a treatment with saturated urea solution—were also applied to the prepared activated carbons. comparison of the treated and untreated activated carbons showed th ...201525460695
quantitative evaluation by attenuated total reflectance infrared (atr-ftir) spectroscopy of the chemical composition of decayed wood preserved in waterlogged conditions.this paper reports on the assessment of lignin and holocellulose by means of atr-ftir analysis and multivariate pls regression. the analysis was conducted on 59 samples coming from different excavations where wood had been preserved in waterlogged conditions. a range of results from different wood species (alnus sp.p., cupressus sempervirens, larix decidua, picea abies, pinus sp.p., quercus sp.p., ulmus sp.p.), states of preservation, waterlogged environments, and burial times are presented. a c ...201525281067
complementarity between microarray and immunoblot for the comparative evaluation of ige repertoire of french and italian cypress pollen allergic patients.cypress pollen represents the primary cause of respiratory allergies in mediterranean areas. patients allergic to cupressus sempervirens pollen (cups) (cpa) can be discriminated on the basis of the immunoglobulin e (ige) binding to a basic 14 kda protein (bp14) or to high-molecular-weight (hmw) glycoproteins only. specific ige repertoires of two differentially exposed cpa cohorts, french and italian, were investigated using an ige microarray system (some known major allergens from several allerg ...201425152053
novel merosesquiterpene exerts a potent antitumor activity against breast cancer cells in vitro and in vivo.this article describes the antitumor properties of a new family of merosesquiterpenes, which were synthesized by diels-alder cycloaddition of the labdane diene trans-communic acid, highly abundant in cupressus sempervirens, or its methyl ester, with the appropriate dienophile. these compounds demonstrated potent cytotoxic activity in vitro against human breast, colon, and lung tumor cells. we highlight the elevated activity (ic50: 0.35 ± 0.10 μm) and specificity (ti: 9) of compound 13 against th ...201424704691
labda-8(17),12,14-trien-19-oic acid contained in fruits of cupressus sempervirens suppresses benign prostatic hyperplasia in rat and in vitro human models through inhibition of androgen and stat-3 signaling.fruit extract of cupressus sempervirens (cs), which is used traditionally to treat benign prostatic hyperplasia (bph)-like urinary symptoms in patients, was scientifically validated for anti-bph activity. the ethanolic fruit extract of cs inhibited proliferation of human bph-stromal cells and the activity was localized to its chloroform-soluble, diterpene-rich fraction. eight major diterpenes isolated from this fraction exhibited moderate to potent activity and the most active diterpene (labda-8 ...201424399792
[cypress pollen allergy].cypress belongs to the cupressaceae family, which includes 140 species with non-deciduous foliage. the most important genera in allergic diseases are cupressus sempervirens or green cypress, cupressus arizonica or blue cypress, juniperus oxycedrus, juniperus communis and thuya. because j. oxycedrus pollinates in october, c. sempervirens in january and february, c. arizonica in february and march, j. communis in april, the symptomatic period is long-lasting. because of global warming, the pollina ...201324314710
molecular detection assay of the bud mite trisetacus juniperinus on cupressus sempervirens in nurseries of central italy.trisetacus juniperinus (nalepa) sensu keifer (acari: eriophyoidea: phytoptidae) causes irregular development of buds, shoot deformations and stunted growth of trees, resulting in a serious threat to nurseries and young stands of cupressus sempervirens l. (mediterranean cypress). recently, some cypress clones selected for their resistance to the fungal canker agent seiridium cardinale (wag.) have shown high susceptibility to the mite. considering its tiny body, its hidden lifestyle inside the bud ...201424030201
essential-oil composition of the tunisian endemic cypress (cupressus sempervirens l. var. numidica trab.).the essential oils isolated from leaves, wood, and cones of the tunisian endemic cypress cupressus sempervirens l. var. numidica trab. collected from three natural populations were characterized by gc-fid and gc/ms analyses. in the wood, leaf, and cone oils, 38, 35, and 26 constituents, representing 94.4, 97.8, and 98.5% of the total oil composition, respectively, were identified. monoterpenes constituted the major fraction of the oils from all organs and for all populations. the oils were found ...201323776018
flammability of some ornamental species in wildland-urban interfaces in southeastern france: laboratory assessment at particle level.assessment of the flammability of ornamental vegetation (particularly hedges) planted around houses is necessary in light of the increasing urbanization of the wildland-urban interfaces (wuis) and the high fire occurrence in such areas. the structure and flammability of seven of the species most frequently planted as hedges in provence (southeastern france) were studied at particle level. spatial repartition of the different types of fuel particles within plants was assessed by means of the cube ...201323765042
description of a new relict eriophyoid mite, loboquintus subsquamatus n. gen. & n. sp. (eriophyoidea, phytoptidae, pentasetacini) based on confocal microscopy, sem, coi barcoding and novel clsm anatomy of internal genitalia.a new pentasetacine mite loboquintus subsquamatus n. gen. & n. sp. was found living under scale-like leaves of 2-3 years old twigs of cupressus sempervirens in montenegro. this mite species possesses a number of morphological features (uncommon teardrop-shaped body, traits of prosoma, atypical primitive anatomy of the genital apparatus and morphological traits of immatures) which clearly distinguish it from all other known eriophyoids. adults of l. subsquamatus have seta vi situated on the anter ...201323494457
relevance of the sea sand disruption method (ssdm) for the biometrical differentiation of the essential-oil composition from conifers.sea sand disruption method (ssdm) is a simple and cheap sample-preparation procedure allowing the reduction of organic solvent consumption, exclusion of sample component degradation, improvement of extraction efficiency and selectivity, and elimination of additional sample clean-up and pre-concentration step before chromatographic analysis. this article deals with the possibility of ssdm application for the differentiation of essential-oils components occurring in the scots pine (pinus sylvestri ...201323418171
extraction of essential oil from cupressus sempervirens: comparison of global yields, chemical composition and antioxidant activity obtained by hydrodistillation and supercritical extraction.in this study, supercritical fluid extraction (sfe) with co2 and hydrodistillation (hd) were compared as methods to isolate the essential oil from cupressus sempervirens. the odour of the oil obtained by sfe at 90 bar and 40°c was very close to the odour of the leaves of c. sempervirens before the extraction. compounds extracted by both sfe and hd were identified by gc-fid and gc-ms. moreover, the difference in the chemical composition obtained by sfe and hd was quite noticeable qualitatively an ...201323316864
chemical analysis and biological activities of cupressus sempervirens var. horizontalis essential oils.safe and effective antioxidants are regarded as a cornerstone for the prevention and treatment of several types of disorders.201323167275
leaf plasticity to light intensity in italian cypress (cupressus sempervirens l.): adaptability of a mediterranean conifer cultivated in the alps.italian cypress (cupressus sempervirens l.) is native to the eastern mediterranean, an area characterised by hot, dry summers and mild winters. over the centuries, however, the species has been introduced into more northerly regions, a long way from its native range. the current, generally warmer climatic conditions brought about by global warming have favoured its cultivation in even more northerly areas in the alps and other european alpine regions. given that not only temperature, but also li ...201223079539
allergomic study of cypress pollen via combinatorial peptide ligand libraries.although cupressus sempervirens (cups) pollen represents one of the main aeroallergens in southern europe, only two cups allergens have yet been identified and reported: cup s 1 and cup s 3. the aim of this study was to identify allergens in cypress pollen using an immuno-proteomic approach. a sequential pollen protein extraction was developed and supplemented by a combinatorial peptide ligand library (cpll) treatment to select low-abundance species. control extracts and cpll eluates have then b ...201222813879
the loss of essential oil components induced by the purge time in the pressurized liquid extraction (ple) procedure of cupressus sempervirens.the influence of different purge times on the effectiveness of pressurized liquid extraction (ple) of volatile oil components from cypress plant matrix (cupressus sempervirens) was investigated, applying solvents of diverse extraction efficiencies. the obtained results show the decrease of the mass yields of essential oil components as a result of increased purge time. the loss of extracted components depends on the extrahent type - the greatest mass yield loss occurred in the case of non-polar ...201222608426
proteomics of cypress pollen allergens using double and triple one-dimensional electrophoresis.italian cypress (cupressus sempervirens, cups) pollen causes allergic diseases in inhabitants of many of the cities surrounding the mediterranean basin. however, allergens of cups pollen are still poorly known. we introduce here a novel proteomic approach based on double one-dimensional gel electrophoresis (d1-de) as an alternative to the 2-de immunoblot, for the specific ige screening of allergenic proteins from pollen extracts. the sequential one-dimensional combination of ief and sds-page ass ...201222287175
arthrobacter cupressi sp. nov., an actinomycete isolated from the rhizosphere soil of cupressus sempervirens.an actinobacterial strain, designated d48(t), was isolated from the rhizosphere soil of a cypress tree collected from mianyang in sichuan province, china. the strain was gram-stain-positive, catalase-positive, oxidase-negative and non-motile, with lysine as the peptidoglycan diagnostic diamino acid and acetyl as the peptidoglycan acyl type. the predominant menaquinone was mk-9(h(2)); small amounts of mk-7(h(2)), mk-10(h(2)) and mk-6 were also present. the major fatty acids were anteiso-c(15:0), ...201222228666
batch and dynamic biosorption of basic dyes from binary solutions by alkaline-treated cypress cone chips.a simple alkaline pre-treatment of cupressus sempervirens cone chips was performed to improve their biosorption capacity towards methylene blue and rhodamine b from aqueous solutions, in batch and continuous modes. biosorption kinetics were determined from single and binary dyes solutions, and properly described by the pseudo-second-order rate model. experimental single-dye equilibrium isotherms fitted the langmuir-freundlich model, with maximum biosorption capacities of 0.68mmol/g for methylene ...201222197337
prevalence and real clinical impact of cupressus sempervirens and juniperus communis sensitisations in tuscan "maremma", italy.the aim was to evaluate the impact of cupressus sempervirens (cs) and juniperus communis (jc) sensitisations in "maremma" in southern tuscany.201421982402
linkage mapping of the mediterranean cypress, cupressus sempervirens, based on molecular and morphological markers.gene mapping for a cupressus species is presented for the first time. two linkage maps for the mediterranean cypress (cupressus sempervirens) varieties, c. sempervirens var. horizontalis and c. sempervirens var. pyramidalis, were constructed following the pseudo-testcross mapping strategy and employing rapd, scar and morphological markers. a total of 427 loci (425 rapds, two scars) representing parents and f(1) progeny were screened for polymorphism with 32 random decamer and two scar primers. a ...201121948752
effectiveness of cupressus sempervirens cones as biosorbent for the removal of basic dyes from aqueous solutions in batch and dynamic modes.the feasibility of using cypress cone chips from cupressus sempervirens as a low-cost biosorbent for the removal of two representative basic dyes, methylene blue (mb) and rhodamine b (rhb), from aqueous solutions was investigated in batch and continuous modes. dyes biosorption was strongly dependent on the solution's ph. sorption kinetics was determined and properly described by the pseudo-second-order rate model. experimental equilibrium isotherms fitted the langmuir model, showing maximum bios ...201020727738
cypress pollen does not cross-react to plant-derived foods.some studies hypothesize the existence of cross-reactivity between allergy to cupressus sempervirens pollen and plant-derived foods. we aimed to assess whether this holds true.201020648776
barriopsis iraniana and phaeobotryon cupressi: two new species of the botryosphaeriaceae from trees in iran.species in the botryosphaeriaceae are well known as pathogens and saprobes of woody hosts, but little is known about the species that occur in iran. in a recent survey of this family in iran two fungi with diplodia-like anamorphs were isolated from various tree hosts. these two fungi were fully characterised in terms of morphology of the anamorphs in culture, and sequences of the its1/its2 regions of the ribosomal dna operon and partial sequences of the translation elongation factor 1-alpha. phy ...200920198156
an insight into some relevant aspects concerning eriophyoid mites inhabiting forests, ornamental trees and shrubs.worldwide a great variety of eriophyoid mites inhabit forest canopy trees and ornamental plants that are used in city parks, squares and boulevards. an analysis of the relevant bibliography portrays only a fragmentary knowledge and the majority of our information concerns the temperate zone. three case studies are presented as examples of different approaches to solve problems connected with eriophyoid mites of forest and ornamental trees. the first example deals with eriophyoids of a temperate ...201019771396
molecular evidence for the natural production of homozygous cupressus sempervirens l. lines by cupressus dupreziana seed trees.paternal apomixis was recently reported in the endangered mediterranean cypress, cupressus dupreziana. this species acts as a surrogate mother for the development of all-paternal embryos from pollen grains. c. dupreziana production of cupressus sempervirens haploid or diploid seedlings from c. sempervirens pollen was also demonstrated. the haploid progeny was derived from the embryogenic development of haploid gametes, but the origin of the diploid progeny remained unknown. to determine the onto ...201019707231
constituents from fruits of cupressus sempervirens.two new phenolic glycosides (1, 2), along with fourteen known compounds (3-16) have been isolated from the fruit of cupressus sempervirens. the structures of these compounds were determined by spectroscopic analysis and were evaluated for their inhibitory activity against glycogen phosphorylase and glucose-6-phosphatase enzymes. compounds 14 showed a moderate inhibition against glucose-6-phosphatase and 15 against glycogen phosphorylase enzymes.201019686818
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