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the influence of roasting procedure on the formation of mutagenic compounds in coffee.mutagenic products can be formed during the processing of food and especially as a result of heat treatment. direct acting mutagenic activity was found in extracts of instant coffee and roasted coffee beans using salmonella typhimurium ta100 in vitro. the mutagenic activities of the four pure coffee varieties examined (coffea arabica santos, coffea arabica columbia, coffee robusta indonesia, coffee robusta camerun) were within the same range. twenty milligrams per plate freeze-dried powder prepa ...19853891559
diterpene composition of oils from arabica and robusta coffee beans and their effects on serum lipids in man.the cholesterol-raising effect of boiled coffee is caused by diterpenes from coffee oil. in order to identify the diterpene responsible, we analysed the diterpene composition of oils from arabica (coffea arabica) and robusta (coffea canephora robusta) beans and their effects on serum lipids and thyroid function. design, subjects, and intervention: during the first 3-week period of a randomized, cross-over trial, 11 healthy, normolipaemic volunteers received per day either 2 g of coffee oil (n = ...19957782725
potential sources of sugar for the phlebotomine sandfly lutzomyia youngi (diptera: psychodidae) in a colombian coffee plantation.sugar-deprived lutzomyia youngi were exposed to each of the five most numerous plant species in a colombian coffee plantation for 24 h and then tested for the presence of fructose by the cold anthrone assay. many flies exposed to coffee (coffea arabica) (up to 55% in an individual test) and guamo (inga edulis) (up to 28.0%) gave positive results. although some flies also took sugar from red-flowered balsam (impatiens balsamina) and bamboo (bambusa angustifolia), none of the insects exposed to pl ...19947979645
microbial diversity during maturation and natural processing of coffee cherries of coffea arabica in brazil.the magnitude and diversity of the microbial population associated with dry (natural) processing of coffee (coffea arabica) has been assessed during a 2-year period on 15 different farms in the sul de minas region of brazil. peptone water-washed samples were taken of maturing cherries on trees (cherries, raisins and dried cherries) and from ground fermentations. the microbial load varied from 3 x 10(4) to 2.2 x 10(9) cfu/cherry with a median value of 1.6 x 10(7) cfu/cherry. the microbial load in ...200011016614
alpha-galactosidase from cultured rice (oryza sativa l. var. nipponbare) cells.the alpha-galactosidase from rice cell suspension cultures was purified to homogeneity by different techniques including affinity chromatography using n-epsilon-aminocaproyl-alpha-d-galactopyranosylamine as the ligand. from 11 l of culture filtrate, 28.7 mg of purified enzyme was obtained with an overall yield of 51.9%. the cdna coding for the alpha-galactosidase was cloned and sequenced. the enzyme was found to contain 417 amino acid residues composed of a 55 amino acid signal sequence and 362 ...200212423882
molecular cloning and functional characterization of three distinct n-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants.caffeine is synthesized from xanthosine through n-methylation and ribose removal steps. in the present study, three types of cdnas encoding n-methyltransferases were isolated from immature fruits of coffee (coffea arabica) plants, and designated as caxmt1, camxmt2, and cadxmt1, respectively. the bacterially expressed encoded proteins were characterized for their catalytic properties. caxmt1 catalyzed formation of 7-methylxanthosine from xanthosine with a k(m) value of 78 microm, camxmt2 catalyze ...200312746542
toxigenic fungi associated with processed (green) coffee beans (coffea arabica l.).processed (green) coffee beans from coffea arabica in brazil were assessed for the presence of aspergillus and penicillium species both before and after surface sterilisation, the aflatoxigenic and ochratoxigenic potential of the isolates and ochratoxin a levels. contamination by aspergillus and penicillium species was found on 96% and 42%, respectively, of 45 samples from 11 localities. after disinfection with 1% sodium hypochlorite, the levels fell to 47% and 24%, respectively. one hundred and ...200312878387
yeast involved in fermentation of coffea arabica in east africa determined by genotyping and by direct denaturating gradient gel electrophoresis.samples of coffea arabica were collected during the different stages of the fermentation from two production sites in tanzania. the yeasts community was identified by genotyping using its-pcr and sequence analysis of the d1/d2 domain of the 26s rrna gene. for confirmation, denaturating gradient gel electrophoresis (dgge) of pcr-amplified 26s rrna gene was performed to detect yeast directly from coffee samples without cultivation. yeast counts were in the range 4.0 x 10(4) - 5.0 x 10(7) cfu/g wit ...200415164358
plant biochemistry: a naturally decaffeinated arabica coffee.the adverse side effects of caffeine have increased the market for decaffeinated coffee to about 10% of coffee consumption worldwide (http://www.ncausa.org), despite the loss of key flavour compounds in the industrial decaffeinating process. we have discovered a naturally decaffeinated coffea arabica plant from ethiopia, a species normally recognized for the high quality of its beans. it should be possible to transfer this trait to commercial varieties of arabica coffee plants by intraspecific h ...200415215853
volatile composition of coffee berries at different stages of ripeness and their possible attraction to the coffee berry borer hypothenemus hampei (coleoptera: curculionidae).the analysis of volatile emissions of coffee berries in different physiological states of ripeness was performed using dynamic headspace and gas chromatography/mass spectrometry analysis for coffea arabica, var. colombia. the composition of the volatiles emitted by coffee berries is dominated by very high levels of alcohols, mainly ethanol, in all stages of ripeness in comparison with other compounds. overripe coffee berries have high volatile emissions and show a composition dominated mainly by ...200415366842
occurrence of gluconacetobacter diazotrophicus in tropical and subtropical plants of western ghats, india.endophytic bacteria were isolated from the tissues of surface sterilized roots, stems, and leaves of fifty different crop plants. phenotypic, biochemical tests and species-specific pcr assay permitted identification of four isolates of gluconacetobacter diazotrophicus from root tissues of carrot (daucus carota l.), raddish (raphanus sativus l.), beetroot (beta vulgaris l.) and coffee (coffea arabica l.). further the plant growth promoting traits such as nitrogenase activity, production of phytoh ...200415462523
farmers' perceptions, knowledge, and management of coffee pests and diseases and their natural enemies in chiapas, mexico.small farmers' perceptions of coffee coffea arabica l. herbivores and their natural enemies, how those perceptions relate to field infestation levels, and pest management practices being implemented by members from two organic and nonorganic coffee grower organizations in the soconusco region, southeastern mexico, were analyzed through an interview survey, diagnostic workshops, and field sampling. the terms pest, disease, and damage were commonly used as synonyms. the major phytophagous species, ...200415568334
application of rnai to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties.the caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct n -methyltransferases, xanthosine methyltransferase (xmt), 7- n -methylxanthine methyltransferase (mxmt; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (dxmt; caffeine synthase). we previously isolated all corresponding cdnas designated as caxmt1 , camxmt1 , camxmt2 and cadxmt1 , respectively, and showed that caffeine was indeed synthesized in vitro by the combination of their gene p ...200415604660
the effects of yeasts involved in the fermentation of coffea arabica in east africa on growth and ochratoxin a (ota) production by aspergillus ochraceus.the effects of pichia anomala, pichia kluyveri and hanseniaspora uvarum predominant during coffee processing on growth of aspergillus ochraceus and production of ochratoxin a (ota) on malt extract agar (mea) and on coffee agar (ca) were studied. the three yeasts were able to inhibit growth of a. ochraceus when co-cultured in mea and ca. growth inhibition was significantly higher on mea than on ca. furthermore, p. anomala and p. kluyveri were found to have a stronger effect on growth of a. ochrac ...200616213049
influence of volatile compounds produced by yeasts predominant during processing of coffea arabica in east africa on growth and ochratoxin a (ota) production by aspergillus ochraceus.the effects of volatile compounds produced during coffee processing by pichia anomala, p. kluyveri and hanseniaspora uvarum on growth of aspergillus ochraceus and production of ochratoxin a (ota) were studied. on malt extract agar (mea) and on coffee agar (ca), exposure of a. ochraceus to the gaseous phase of malt yeast glucose peptone (mygp) plates inoculated with p. anomala, p. kluyveri and h. uvarum inhibited fungal growth, with the two pichia spp. showing the strongest effect. the main ester ...200516240461
design and evaluation of traps for idiarthron subquadratum (orthoptera: tettigoniidae) with farmer participation in coffee plantations in chiapas, mexico.three new types of traps designed and made by farmers were evaluated for capture of idiarthron subquadratum saussure & pictet. bag, sack, and fabric traps were compared with previously used bamboo internode traps. a participatory methodology was used involving farmer interviews and workshops to design and make the traps. farmer participation was useful for obtaining information on perceptions, knowledge, and control activities of i. subquadratum. the bag trap captured a greater number of individ ...200516022311
[growth and photosynthetic characteristics of field-grown coffea arabica under different watering and fertilization managements].straw mulching and drip irrigation have been widely used for coffee production. in order to know if these techniques are suitable in yunnan, five-year-old coffee plants were separated into 8 groups to grow in the field under 2 fertilization rates (low and high) and 4 watering regimes: dry straw mulching (m), drip irrigation (i), drip irrigation + straw mulching (mi) and control treatment (ck), and the effects of various watering and fertilization treatments on the growth and photosynthetic chara ...200415506100
comparison of bean biochemical composition and beverage quality of arabica hybrids involving sudanese-ethiopian origins with traditional varieties at various elevations in central america.for buyers of arabica coffee (coffea arabica l.) in central america, elevation and variety are important indicators of quality. we compared coffee produced by three types of varieties established in various trials at elevations ranging from 700-1600 m in three countries (el salvador, costa rica and honduras). arabica hybrids resulting from crosses of sudanese-ethiopian origins with either traditional varieties or with introgressed lines derived from the hybrid of timor (c. arabica x coffea canep ...200616740499
introgression molecular analysis of a leaf rust resistance gene from coffea liberica into c. arabica l.leaf rust caused by the fungus hemileia vastatrix is the most devastating disease of arabica coffee ( coffea arabica). therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. the purpose of the present work was to gain insight into the mechanism of introgression into c. arabica of a leaf rust resistance gene from c. liberica (i.e. s(h)3 resistance factor) and to identify associated molecular markers. an f(2) progeny (i.e. 101 i ...200415241596
genetic diversity of forest arabica coffee (coffea arabica l.) in ethiopia as revealed by random amplified polymorphic dna (rapd) analysis.genetic diversity within the forest coffea arabica l. gene pool in ethiopia has not been extensively examined with molecular markers. in the present study, a total of 75 polymorphic rapd bands generated by twelve random primers were used to assess genetic diversity among 144 genotypes representing 16 c. arabica populations. the number of polymorphic bands detected with each primer ranged from 2 to 9 with a mean of 6.25 bands per primer. banding patterns ranged in percentage polymorphism from 37% ...200312830983
3-methylbutanoyl and 3-methylbut-2-enoyl disaccharides from green coffee beans (coffea arabica).three 3-methylbutanoyl and 3-methylbut-2-enoyl disaccharides isolated from green coffee beans (coffea arabica) were identified as 3-methylbutanoyl-1-o-beta-d-glucopyranosyl-beta-d-apiofuranoside, 3-methylbutanoyl-6-o-alpha-d-glucopyranosyl-beta-d-fructofuranoside, and 3-methylbut-2-enoyl-1-o-beta-d-glucopyranosyl-beta-d-apiofuranoside. the structures were established by one- and two-dimensional 1h and 13c nmr spectra as well as by esi ms/ms spectra.200212031434
parasitoids of medfly, ceratitis capitata, and related tephritids in kenyan coffee: a predominantly koinobiont assemblage.arabica coffee was sampled from two sites in the central highlands of kenya (rurima, ruiru) and one site on the western side of the rift valley (koru). three species of ceratitidine tephritidae, ceratitis capitata (wiedemann), c. rosa karsch and trirhithrum coffeae bezzi, were reared from sites in the central highlands, and an additional species, c. anonae graham, was recovered from the western-most site. ten species of parasitic hymenoptera were reared from these tephritids. the parasitoid asse ...200011107253
[coffea arabica--production, botanical classification, new components and active agents and their pharmacological effects].the present paper surveys the latest knowledge about coffee grains (coffea arabica) as an important part of our everyday life. it lists the botanical classification of its principal species, such as coffea arabica, coffea robusta, and coffea liberica, the most important improved cultivars of production species in the world. the review paper also discusses the economic aspect, lists the most important regions of production, the optimal and farming technological conditions. it points out the occur ...200010953453
an effective and low-cost culture medium for isolation and growth of xylella fastidiosa from citrus and coffee plants.buffered charcoal-yeast extract medium (bcye) has been used for isolation of xylella fastidiosa from citrus (citrus sinensis) and coffee (coffea arabica) plants affected by citrus variegated chlorosis (cvc) and coffee leaf scorch (cls). bcye is composed of aces (2-[2-amino-2oxoethyl) amino]-ethanesulfonic acid) buffer, activated charcoal, yeast extract, l-cysteine, ferric pyrophosphate, and agar. aces buffer is costly and not always commercially available in brazil, and the l-cysteine and ferric ...200617111277
isolation of a paracoccidioides brasiliensis strain from the soil of a coffee plantation in ibiá, state of minas gerais, brazil.paracoccidioides brasiliensis has rarely been isolated from its habitat in rural areas. in order to investigate the hypothesis that human infection with this fungus is linked to coffee plantations (coffea arabica), material was collected monthly over a period of 1 year from farms in the town of ibiá, state of minas gerais, brazil. a total of 760 samples of soil, coffee leaves and fruits was cultured and inoculated into mice. a fungus isolated from the liver of a mouse inoculated with soil showed ...19989776810
biochemistry and toxicology of the diterpenoid glycoside atractyloside.atractyloside (atr) is a diterpenoid glycoside that occurs naturally in plants (many of which are used in ethnomedicines) found in europe, africa, south america, asia and the far east. it is also present in animal grazing forage. atr (and its analogues) may be present at levels as high as 600 mg/kg dried plant material. consumption of the plants containing atr or carboxyatractyloside (carboxyatr) has caused fatal renal proximal tubule necrosis and/or centrilobular hepatic necrosis in man and far ...19989651051
[susceptibility of six arabic coffee cultivars to fruit flies (diptera: tephritoidea) under shaded and unshaded organic management in valença, state of rio de janeiro, brazil].the infestation indices by fruit flies were determined for six cultivars of coffea arabica l. in shaded and unshaded systems under organic management. the experiment was set in a completely randomized design with a split-split-plot arrangement and four replicates. a 250g-sample of maturing fruits per plot was harvested in may 2005. the cultivars icatu amarelo and catucaí amarelo were the least susceptible to attack by tephritids in both systems. as for lonchaeids, oeiras, catucaí amarelo and cat ...200717607461
coffea arabica l., a new host plant for acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria.acetobacter diazotrophicus was isolated from coffee plant tissues and from rhizosphere soils. isolation frequencies ranged from 15 to 40% and were dependent on soil ph. attempts to isolate this bacterial species from coffee fruit, from inside vesicular-arbuscular mycorrhizal fungi spores, or from mealybugs (planococcus citri) associated with coffee plants were not successful. other acid-producing diazotrophic bacteria were recovered with frequencies of 20% from the coffee rhizosphere. these n2-f ...19979293018
dietary fiber from coffee beverage: degradation by human fecal microbiota.arabinogalactans and galactomannans from coffee beverages are part of the dietary fiber complex. chemical structures and fermentability of soluble dietary fiber obtained from a standard filter coffee beverage (coffea arabica, origin colombia, medium roasted) by human intestinal bacteria were investigated. one cup (150 ml) of filter coffee contained approximately 0.5 g of soluble dietary fiber (enzymatic-gravimetric methodology), 62% of which were polysaccharides. the remainder was composed of ma ...200717658822
[use of a coffea arabica tosta extract for the prevention and therapy of polyfactorial infectious diseases in newborn calves].two studies have been carried out to evaluate the prophylactic and therapeutical effect of a 30%-extract from the coffee-bean seeds coffeae arabicae on infectious diseases in newborn calves. 1. within a large cattle-herd, which endemically showed a high proportion of infections within the gastroenteric and/or respiratory systems in calves, a randomised placebo-controlled double-blind study has been done. 50 newborn calves were given a subcutaneous injection of 10 ml coffea-preparation 30% on fir ...19969035968
semiquantitative evaluation of dermal exposure to granulated insecticides in coffee (coffea arabica l.) crop and efficiency of individual protective equipment. 19968875843
host breadth and parasitoids of fruit flies (anastrepha spp.) (diptera: tephritidae) in puerto rico.twenty fruit species representing 12 families were collected from various regions in western puerto rico and monitored for the emergence of anastrepha spp. pupae. we collected 14,154 tephritid pupae from 16 fruit species representing 10 families. the relative infestations of these fruits (pupae per kilogram of fruit) were recorded. recorded host ranges were not in complete agreement with those reported in the literature. this host-use pattern should give pause to regulators of fruit importation ...200818348802
impact of the invasive plant syzigium jambos (myrtaceae) on patterns of understory seedling abundance in a tropical premontane forest, costa rica.habitat fragmentation, along with other human-induced disturbances, increase the vulnerability of native habitats to be invaded by aggressive, ecologically released, exotic species. syzigium jambos (l.) alston (myrtaceae, rose apple) is an important invader still spreading throughout hawaii, the antilles, central and south america. this study examines the effects of s. jambos on plant understory diversity in a 25 ha tropical premontane moist forest in atenas, alajuela, costa rica, a protected wa ...200618494312
investigation of the effect of coffee lipids on serum cholesterol in hamsters.the effect of coffee total lipids (ctl), coffee non-saponifiable matter (nsm) and coffee diterpene alcohols (dta) extracted from coffea arabica beans on serum cholesterol in adult male syrian hamsters was examined. the animals were fed either a commercial laboratory chow diet (study 1), containing 5% fat and low in saturated fat (1.46 g/100 g diet) and cholesterol (0.03 g/100 g diet) or a semi-synthetic diet (study 2) set in gelatine, containing 10% fat and high in saturated fat (4 g/100 g diet) ...19957896229
tobacco, cocoa, coffee, and ragweed: cross-reacting allergens that activate factor-xii-dependent pathways.a glycoprotein antigen has been isolated from cured tobacco leaves (tgp-l) nicotiana tabacum) and from cigarette smoke condensate (tgp-csc) to which approximately one-third of human volunteers, smokers and non-smokers, exhibit immediate cutaneous hypersensitivity. tgp-l and tgp-csc contain polyphenol haptens that activate the factor xii (hageman factor) dependent pathways of coagulation, fibrinolysis, and kinin generation in normal human plasma. the purpose of this communication is to describe t ...19817295998
natural antioxidants.the constant exposure of the skin to oxidative stress results in damage to cellular dna and cell membrane lipids and proteins. to combat this problem, the skin contains a number of antioxidants that protect against oxidative injury. however, these cutaneous antioxidants can be depleted by sun exposure and environmental insults, resulting in an overload of oxidation products. thus, topical antioxidants that replenish the antioxidant capacity of the skin have the potential to prevent oxidative dam ...200818681153
incidence of microflora and of ochratoxin a in green coffee beans (coffea arabica).coffee is produced in tropical countries around the equator where climatic conditions are favourable for fungal development and mycotoxin production; however, mycotoxins do not only occur in the tropical countries. the aim was to evaluate the mycoflora and possible incidence of ochratoxin a (ota) in 60 samples of green coffee beans from brazil. the mycological evaluation was carried out using a conventional method and the ota was determined using sequential phenyl silane and immunoaffinity colum ...200314726276
isolation, identification and toxigenic potential of ochratoxin a-producing aspergillus species from coffee beans grown in two regions of thailand.in 2006 and 2007, 32 thai dried coffee bean samples (coffea arabica) from two growing sites of chiang mai province, and 32 thai dried coffee bean samples (coffea canephora var. robusta) from two growing sites of chumphon province, thailand, were collected and assessed for the distribution of fungi with the potential to produce ochratoxin a (ota). the overall percentage of fungal contamination in coffee was 98% and reduced to 60% after surface disinfection. there were remarkable ecological differ ...200818819720
an evaluation of the genetic diversity of xylella fastidiosa isolated from diseased citrus and coffee in são paulo, brazil.abstract strains of xylella fastidiosa, isolated from sweet orange trees (citrus sinensis) and coffee trees (coffea arabica) with symptoms of citrus variegated chlorosis and requeima do café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (pcr) and enterobacterial repetitive intergenic consensus pcr assays. these strains were also indistinguishable with a previously described pcr assay that distinguished the citrus strains from all other ...200118943950
genetic variability and host specialization in the latin american clade of ceratocystis fimbriata.abstract the ceratocystis fimbriata complex includes many undescribed species that cause wilt and canker diseases of many economically important plants. phylogenetic analyses of dna sequences have delineated three geographic clades within ceratocystis fimbriata. this study examined host specialization in the latin american clade, in which a number of lineages were identified using sequences of the internal transcribed spacer (its) region of the rdna. three host-associated lineages were identifie ...200318944327
impact of the coffea canephora gene introgression on beverage quality of c. arabica.lines of coffea arabica derived from the timor hybrid (hybrid between c. arabica and c. canephora) are resistant to coffee leaf rust (hemileia vastatrix) and to the nematode meloidogyne exigua. the introgression of c. canephora resistance genes is suspected of causing a drop in beverage quality. coffee samples from pure lines, compared in a trial 1, and from f1 hybrids and parental lines from a half-diallel trial in a trial 2, were studied for beverage quality, chemical composition and amount of ...200312750771
in vitro antioxidant and ex vivo protective activities of green and roasted coffee.the antioxidant properties of green and roasted coffee, in relation to species (coffea arabica and coffea robusta) and degree of roasting (light, medium, dark), were investigated. these properties were evaluated by determining the reducing substances (rs) of coffee and its antioxidant activity (aa) in vitro (model system beta-carotene-linoleic acid) and ex vivo as protective activity (pa) against rat liver cell microsome lipid peroxidation measured as tba-reacting substances. rs of c. robustasam ...200010820041
effect of shade on arabica coffee berry disease development: toward an agroforestry system to reduce disease impact.coffee berry disease (cbd), caused by colletotrichum kahawae, is a major constraint for arabica coffee cultivation in africa. the disease is specific to green berries and can lead to 60% harvest losses. in cameroon, mixed cropping systems of coffee with other crops, such as fruit trees, are very widespread agricultural practices. fruit trees are commonly planted at random on coffee farms, providing a heterogeneous shading pattern for coffee trees growing underneath. based on a recent study of cb ...200819000007
[epidemiology of anthracnosis and eye spot disease, conclusions for its prognosis of occurrence and its control in the republic of cuba].the course of attacks by anthracnosis and by the eye spot disease on branches, leaves, and fruits of coffea arabica l. were investigated in cuba. the daily precipitation and the shading are most influential for both diseases. there are marked differences in the attacks between the rainy season (highest attacks) and dry season (decreased attacks). lack of shading increases the susceptibility of the plants to the eye spot disease. under the conditions in cuba, unshaded plantations exhibited an inc ...19827126133
[temporal and spatial distribution of ants in a light gradient, in a coffee agroforestry system, turrialba, costa rica].shade trees are frequently present in coffee (coffea arabica l.) agroforestry systems of mesoamerica. these systems can harbor a rich entomofauna, including ants, which could be predators of key pests in these systems. however, the role of shade on the distribution and abundance of these ants is unknown, yet such knowledge could suggest guidelines for manipulating certain environmental conditions of their habitat, thereby achieving their conservation and increase. therefore, we studied the effec ...200719086397
in vitro fasciolicide activity of some plant extracts against newly excysted flukes.the aim of the present study was to evaluate, under in vitro conditions, the activity of 20 plant extracts against newly excysted fasciola hepatica metacercariae. the plants were selected on the basis of anthelmintic activity, traditional use, availability, solubility, and permanence in the liver. for the in vitro evaluation, 24-well nunc-immunoplates containing rpmi-1640 culture medium bovine sera plus antibiotics were used. extract concentrations of 500 and 50 mg/ml were placed in triplicate. ...200819120205
5-(hydroxymethyl)-2-furfural: a selective inhibitor of dna polymerase lambda and terminal deoxynucleotidyltransferase.5-(hydroxymethyl)-2-furfural (hmf), a pyrolysate of carbohydrate isolated from instant coffee (coffea arabica l.), selectively inhibits the activities of mammalian dna polymerase lambda (pol lambda) and terminal deoxynucleotidyltransferase (tdt) which are family x pols, in vitro. the compound influenced neither the activities of replicative dna polymerases such as alpha, delta, and epsilon, nor even the activity of pol beta which is from the same family and thought to have a very similar three-d ...200616405901
efficient production of agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots.the possibility of rapid validation and functional analysis of nematode resistance genes is a common objective for numerous species and particularly for woody species. in this aim, we developed an agrobacterium rhizogenes-mediated transformation protocol for coffea arabica enabling efficient and rapid regeneration of transformed roots from the hypocotyls of germinated zygotic embryos, and the subsequent production of composite plants. the a. rhizogenes strain a4rs proved to be the most virulent. ...200616596429
characterizing plasma membrane h+-atpase in two varieties of coffee leaf (coffea arabica l.) and its interaction with an elicitor fraction from the orange rust fungus (h. vastatrix berk and br.) race ii.early intercellular signaling in coffea arabica l.-hemileia vastatrix host-pathogen interaction was studied, using inside-out plasma membrane from two varieties of coffee leaf and a fungal fraction to determine the plant's biochemical responses. microsomal pellets (100,000 x g) from the susceptible (caturra) and resistant (colombia) coffee leaf varieties were purified by partitioning in two-polymer dex (6.3% w/w) and peg (6.3% w/w) system aqueous phase. fungal material was obtained from orange r ...200616781870
variability of meloidogyne exigua on coffee in the zona da mata of minas gerais state, brazil.minas gerais is the major coffee-producing state of brazil, with 28% of its production coming from the region of zona da mata. four major species of root-knot nematode attacking coffee (meloidogyne incognita, m. paranaensis, m. coffeicola, and m. exigua) have been reported from brazil. to determine the variability in meloidogyne spp. occurring in that region, 57 populations from 20 localities were evaluated for morphological, enzymatic, and physiological characteristics. according to the perinea ...200519262880
pectin degrading enzymes in yeasts involved in fermentation of coffea arabica in east africa.the ability of six strains of pichia anomala, four strains of pichia kluyveri and two strains of hanseniaspora uvarum predominant during coffee processing to produce polygalacturonase (pg), pectin esterase (pe) and pectin lyase (pl) in yeast polygalacturonic acid medium (ypa) and in coffee broth (cb) was studied. for comparison, a reference strain of kluyveromyces marxianus cct 3172 isolated from cocoa and reported to produce high amount of pg was included. initial screening of pg activity using ...200616784790
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
antiadhesive effect of green and roasted coffee on streptococcus mutans' adhesive properties on saliva-coated hydroxyapatite beads.green and roasted coffees of the two most used species, coffea arabica and coffea robusta, several commercial coffee samples, and known coffee components were analyzed for their ability to interfere with streptococcus mutans' sucrose-independent adsorption to saliva-coated hydroxyapatite (ha) beads. all coffee solutions showed high antiadhesive properties. the inhibition of s. mutans' adsorption to ha beads was observed both when coffee was present in the adsorption mixture and when it was used ...200211853508
gamma-tocopherol as a marker of brazilian coffee (coffea arabica l.) adulteration by corn.the adulteration of coffee with cereals, coffee twigs, etc. is apparently widespread in brazil with corn being considered the most widely used. no adequate methods are available to detect such contamination in commercial coffee. a new method, based on high-performance liquid chromatography (hplc) tocopherol determination was developed to detect coffee adulteration by corn. percentages of alpha-, beta-, gamma-, and delta-tocopherol determined by hplc in six coffee varieties were 29.0, 61.7, 3.3, ...200717602658
description of pratylenchus gutierrezi n. sp. (nematoda: pratylenchidae) from coffee in costa rica.a lesion nematode, pratylenchus gutierrezi n. sp., collected from the roots of coffee in the central plateau of costa rica, is described and illustrated. its relationships to pratylenchus flakkensis, p. similis, and p. gibbicaudatus, the only other species of the genus having two head annules, males, or spermatheca with sperm, and an annulated tail terminus, is discussed. other distinctive characters are its posterior vulva (mean of 80%); its prominently rounded stylet knobs, low head, and subcy ...199219282999
inter-simple sequence repeat (issr) variation in forest coffee trees (coffea arabica l.) populations from ethiopia.genetic variation of forest coffee trees (coffea arabica l.) from four regions of ethiopia was investigated using inter-simple sequence repeat (issr) markers. a total of 160 individuals representing 16 populations were sampled. eleven issr primers amplified a total of 123 fragments of which 31 fragments (25%) were polymorphic. estimate of total gene diversity (ht), and the coefficient of genetic differentiation (gst) were 0.37 and 0.81, respectively. this indicates that most of the variability i ...200516134334
the first committed step reaction of caffeine biosynthesis: 7-methylxanthosine synthase is closely homologous to caffeine synthases in coffee (coffea arabica l.).in coffee and tea plants, caffeine is synthesized from xanthosine via a pathway that has three methylation steps. we identified and characterized the gene encoding the enzyme for the first methylation step of caffeine biosynthesis. the full-length cdna of coffee tentative caffeine synthase 1, ctcs1, previously isolated by the rapid amplification of cdna ends was translated with an escherichia coli expression system and the resultant recombinant protein was purified using ni-nta column. the prote ...200312860386
penicillium species endophytic in coffee plants and ochratoxin a production.tissues from coffea arabica, c. congensis, c. dewevrei and c. liberica collected in colombia, hawaii and at a local plant nursery in maryland were sampled for the presence of fungal endophytes. surface sterilized tissues including roots, leaves, stems and various berry parts were plated on yeast-malt agar. dna was extracted from a set of isolates visually recognized as penicillium, and the internal transcribed spacer region and partial lsu-rdna was amplified and sequenced. comparison of dna sequ ...200616800302
nickel elicits a fast antioxidant response in coffea arabica cells.the antioxidant responses of coffee (coffea arabica l.) cell suspension cultures to nickel (ni) were investigated. ni was very rapidly accumulated in the cells and the accumulation could be directly correlated with the increase of nicl(2) concentration in the medium. at 0.05 mm nicl(2) growth was stimulated, but at 0.5 mm nicl(2), the growth rate was reduced. an indication of alterations in the presence of reactive oxygen species was detected by an increase in lipid peroxidation at 0.5 mm nicl(2 ...200616806955
a simple method to measure effective catalase activities: optimization, validation, and application in green coffee.oxidative metabolism in coffee cherries during maturation appears to be regulated by the timely expression of redox enzymes such as catalase (cat), peroxidase (pod), and polyphenoloxidase (ppo). among these enzymes, cat is suspected to contribute significantly in setting the redox status of the healthy cherry and the processed bean. the initial redox status of the green bean might further control the nature and dynamics of reactions induced by roasting and eventually quality aspects of the end p ...200717141173
[diversity of hemiptera auchenorrhyncha in citrus, coffee and a fragment of native forest of the state of são paulo].the population of hemiptera auchenorrhyncha was studied in sweet citrus groves (citrus sinensis), coffee plantations (coffea arabica) and a semi-deciduous seasonal forest with shrub physiognomy in bebedouro, sp, to evaluate the influence of the natural ecosystem on the species composition of the agroecosystems. monitoring was carried out by using yellow stick cards, which were replaced every 15 days and all auchenorrhyncha collected were counted and identified. seven families, 11 subfamilies and ...200920098931
biodiversity and new records of microfungi in the ruhrarea (north rhine westfalia), germany.during our investigations of the microflora in nrw (duisburg, düsseldorf and essen incl. the greenhouse of the botanical garden) in 2007 and 2008, we were able to collect and identify about 55 species on trees, bushes and ornamental plants as parasites and saprophytes. some of these species are new for germany or have been only rarely found until now. most of the species belong the ascomycotina, basidiomycotina and deuteromycotina for example arthrocladiella mougeotii (lév.) vassilkov. on lycium ...200920222564
the coffee diterpene kahweol sensitizes trail-induced apoptosis in renal carcinoma caki cells through down-regulation of bcl-2 and c-flip.kahweol, a coffee-specific diterpene, found in the beans of coffea arabica, has potent anti-carcinogenic, anti-tumor, and anti-inflammatory properties. trail is a potential anti-cancer compound that induces apoptosis in a wide variety of cancer cells, but not in most normal human cell types. in the present study, we show that kahweol sensitizes human renal cancer cells, but not normal human mesangial cells, to trail-mediated apoptosis. moreover, treatment with a combination of kahweol and trail ...201020403343
characterization and fermentability of an ethanol soluble high molecular weight coffee fraction.brews from differently roasted arabica coffees were shown to contain 8-12% ethanol soluble substances with molecular masses greater than 2 kda, possibly contributing to their dietary fiber contents. about 13% of these substances were nondigestible carbohydrates, mainly arabinogalactans. the nondigestible high molecular weight ethanol soluble fraction (hesf) of the medium roasted coffee brew was further characterized and subjected to in vitro fermentation with human fecal bacteria. in addition to ...200818558695
endophytic bacteria in coffea arabica l.eighty-seven culturable endophytic bacterial isolates in 19 genera were obtained from coffee plants collected in colombia (n = 67), hawaii (n = 17), and mexico (n = 3). both gram positive and gram negative bacteria were isolated, with a greater percentage (68%) being gram negative. tissues yielding bacterial endophytes included adult plant leaves, various parts of the berry (e.g., crown, pulp, peduncle and seed), and leaves, stems, and roots of seedlings. some of the bacteria also occurred as ep ...200516187260
coffee (coffea arabica l.) genes early expressed during infection by the rust fungus (hemileia vastatrix).summary the beverage cash crop coffee (coffea arabica l.) is subject to severe losses caused by the rust fungus hemileia vastatrix. in naturally resistant coffee plants, a specific hypersensitive reaction (hr) may be elicited early to stop fungal infection. to isolate host genes involved in hr, we undertook an expressed sequence tags (ests) analysis. two cdna libraries were constructed using suppression subtractive hybridization (ssh) and 527 non-redundant ests were generated from 784 randomly p ...200420565627
antioxidant metabolism of coffee cell suspension cultures in response to cadmium.the antioxidant responses of coffee (coffea arabica l.) cell suspension cultures to cadmium (cd) were investigated. cd accumulated very rapidly in the cells and this accumulation was directly correlated with an increase in applied cdcl(2) concentration in the external medium. at 0.05mm cdcl(2), growth was stimulated, but at 0.5mm cdcl(2), the growth rate was reduced. an alteration in activated oxygen metabolism was detected by visual analysis as well as by an increase in lipid peroxidation at th ...200616762393
novel nitrogen-fixing acetic acid bacteria, gluconacetobacter johannae sp. nov. and gluconacetobacter azotocaptans sp. nov., associated with coffee plants.diazotrophic bacteria were isolated, in two different years, from the rhizosphere and rhizoplane of coffee (coffea arabica l.) plants cultivated in mexico; they were designated as type dor and type sad isolates. they showed characteristics of the family acetobacteraceae, having some features in common with gluconacetobacter (formerly acetobacter) diazotrophicus, the only known n2-fixing species of the acetic acid bacteria, but they differed from this species with regard to several characteristic ...200111491326
molecular ecology and polyphasic characterization of the microbiota associated with semi-dry processed coffee (coffea arabica l.).this work was aimed at isolating and identifying the microbiota present during the semi-dry method of coffee processing using polyphasic methods and to evaluate microbial diversity with pcr-dgge. samples of coffea arabica l. were collected during different processing stages in southern minas gerais, brazil. the bacterial and fungal isolates were phenotypically characterised and grouped according to the ardra technique, in which the 16-23s and its1-5.8s regions of the rdna were sequenced for spec ...201020832694
effective sampling range of a synthetic protein-based attractant for ceratitis capitata (diptera: tephritidae).studies were conducted in honduras to determine effective sampling range of a female-targeted protein-based synthetic attractant for the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae). multilure traps were baited with ammonium acetate, putrescine, and trimethylamine lures (three-component attractant) and sampled over eight consecutive weeks. field design consisted of 38 traps (over 0.5 ha) placed in a combination of standard and high-density grids to facilitate ge ...201021061993
coffee farm diversity and landscape features influence density of colonies of atta cephalotes (hymenoptera: formicidae).the density of colonies of leaf-cutting ants, atta cephalotes l. (hymenoptera: formicidae), was measured and compared among coffee (coffea arabica l.) plantations in five management categories: monoculture conventional, diversified conventional, diversified organic, highly diversified conventional, and highly diversified organic. twenty-four small farms (<4 ha) in turrialba, costa rica, were included in this study. within-farm and off-farm (landscape) variables were measured and tested for their ...201121404854
ochratoxin formation in aspergillus ochraceus with particular reference to spoilage of coffee.production of ochratoxin on media by eight isolates of aspergillus ochraceus from coffee or its processing environment in india, indonesia, kenya, and brazil, and seven brazilian isolates from other commodities, has been compared with yields in shaken fermentation on shredded wheat and coffee (coffea arabica). shredded wheat most consistently allowed expression of biosynthesis of ochratoxins a and b in yields up to 3.5% of the dry product. culture on artificial media was an unreliable predictor ...200010857930
life history of amblyseius herbicolus (chant) (acari: phytoseiidae) on coffee plants.the predaceous mite amblyseius herbicolus (chant) is the second most abundant phytoseiid on coffee plants (coffea arabica l), after euseius alatus deleon, in lavras, mg, brazil, associated to the vector of the coffee ring spot virus, brevipalpus phoenicis (geijskes) (acari: tenuipalpidae). its life history was studied taking into account biological aspects, life table, predatory activity and functional and numerical responses in relation to the density of the prey. the adult female has longevity ...200717607463
transmission efficiency of xylella fastidiosa by sharpshooters (hemiptera: cicadellidae) in coffee and citrus.xylella fastidiosa (wells, raju, hung, weisburg, mandelco-paul, and brenner) is a bacterial pathogen transmitted by several sharpshooters in two tribes of cicadellinae (proconiini and cicadellini). here, we compared the transmission efficiency of x. fastidiosa in coffee (coffea arabica l.) and citrus [citrus sinensis (l.) osbeck] by cicadellini [bucephalogonia xanthophis (berg) and dilobopterus costalimai young] and proconiini [homalodisca ignorata melichar and oncometopia facialis (signoret)] s ...200818767717
succession of bacterial and fungal communities during natural coffee (coffea arabica) fermentation.bacteria, yeasts and filamentous fungi were isolated during natural coffee processing. bacteria were isolated in greater numbers at the beginning of the fermentation, when the moisture of the coffee beans was around 68%. gram-positive bacteria represented 85.5% of all bacteria isolated, and bacillus was the predominant genus (51%). gram-negative species of the genera serratia, enterobacter and acinetobacter were also found. approximately 22% of 940 randomly chosen isolates of microorganisms were ...200818954729
Identification and characterization of the Non-race specific Disease Resistance 1 (NDR1) orthologous protein in coffee.Leaf rust, which is caused by the fungus Hemileia vastatrix (Pucciniales), is a devastating disease that affects coffee plants (Coffea arabica L.). Disadvantages that are associated with currently developed phytoprotection approaches have recently led to the search for alternative strategies. These include genetic manipulations that constitutively activate disease resistance signaling pathways. However, molecular actors of such pathways still remain unknown in C. arabica. In this study, we have ...201122023696
effects of pratylenchus brachyurus and p. coffeae on seedlings of coffea arabica.two experiments were carried out to evaluate the effects of pratylenchus brachyurus and p. coffeae on coffea arabica. the first experiment was conducted in a greenhouse to determine the effects of pratylenchus brachyurus and p. coffeae on seedlings of coffea arabica cv. mundo novo. both pratylenchus spp. reduced the growth of coffee seedlings. higher contents of soluble sugars were detected in the leaves of infected plants. the reproduction rate of p. brachyurus was very low on cv. mundo novo, i ...199819274229
cascading indirect effects in a coffee agroecosystem: effects of parasitic phorid flies on ants and the coffee berry borer in a high-shade and low-shade habitat.nonconsumptive effects (nce) of parasites on hosts vary with habitat complexity thereby modifying trait-mediated effects on lower trophic levels. in coffee agroecosystems, pseudacteon sp. phorid fly parasites negatively affect azteca instabilis f. smith ants via nce thereby indirectly benefiting prey. it is unknown how differences in habitat complexity influence azteca-phorid interactions or how phorids affect the coffee berry borer (hypothenemus hampei ferrari), an important pest of coffee (cof ...201122251635
characterization of high molecular weight coffee fractions and their fermentation by human intestinal microbiota.to investigate the structure and fermentability of high m(r) components of coffee brews by human gut bacteria arabica coffee samples of three different degrees of roast (light, medium, and dark) were used for drip brew preparations and fractionation by ultrafiltration with different m(r) cut-offs. total carbohydrates of the fractions ranged from 28.6 g/100 g to 56.7 g/100 g. galactomannans and arabinogalactans were the main polysaccharides and made up between one-fourth and one-half of the respe ...200918985654
long-term caffeine consumption reverses tumor-induced suppression of the innate immune response in adult mice.caffeine (1,3,7-trimethylxanthine), the active principle alkaloid of coffee ( coffea arabica) and tea ( camellia sinensis) possesses a restraining effect on tumor-induced suppression of the specific immune response in adult mice. the present study deals with the effect of long-term consumption of caffeine in the development of ehrlich ascites carcinoma (eac) cells in adult swiss female mice, in relation to the innate immune response and tumor growth. although the consumption of caffeine alone fo ...200819016405
effect of aldicarb, ethoprop, and carbofuran on control of coffee root-knot nematode, meloidogyne exigua.egg hatch of meloidogyne exigua was significantly inhibited in 14 days pretreatment with aldicarb, ethoprop, or carbofnran at concentrations higher than 0.1 mug/ml; these eggs were found to delay hatch in 19 days posttreatment in ethoprop. aldicarb and carbofuran solutions at concentrations greater than 0.1 mug/ml significantly decreased the motility and the life span of the second-stage juveniles; aldicarb was more toxic than carbofuran to the nematode. in a field test, aldicarb (temik 10g), et ...198319295839
prediction of sensory properties of brazilian arabica roasted coffees by headspace solid phase microextraction-gas chromatography and partial least squares.volatile compounds in fifty-eight arabica roasted coffee samples from brazil were analyzed by spme-gc-fid and spme-gc-ms, and the results were compared with those from sensory evaluation. the main purpose was to investigate the relationships between the volatile compounds from roasted coffees and certain sensory attributes, including body, flavor, cleanliness and overall quality. calibration models for each sensory attribute based on chromatographic profiles were developed by using partial least ...200919185116
new coffee plant-infecting xylella fastidiosa variants derived via homologous recombination.xylella fastidiosa is a xylem-limited phytopathogenic bacterium endemic to the americas that has recently emerged in asia and europe. although this bacterium is classified as a quarantine organism in the european union, importation of plant material from contaminated areas and latent infection in asymptomatic plants have engendered its inevitable introduction. in 2012, four coffee plants (coffea arabica and coffea canephora) with leaf scorch symptoms growing in a confined greenhouse were detecte ...201626712553
isolation of cdna encoding the catalytic site of phosphatidylinositol-specific phospholipase c from coffea arabica l.: recombinant expression and peptide purification.a cdna encoding the catalytic site of a phosphatidylinositol-specific phospholipase c (pi-plc) was isolated from coffea arabica suspension cells. the cdna (designated caplc) encodes a polypeptide of 308 amino acids, containing the catalytic x and y domains, and has 99% identity to the soybean gene. recombinant caplc protein was expressed in escherichia coli, purified, and used to produce a polyclonal antibody. the peptide has a molecular mass of 27 kda on sodium dodecyl sulfate-polyacrylamide ge ...200819513191
quantification of coffea arabica and coffea canephora var. robusta in roasted and ground coffee blends.this study reports direct quantification of arabica in roasted and ground coffee blends of coffea arabica and coffea canephora var. robusta. (1)h-nmr analysis of water extracts of coffee blends were combined with multivariate statistical analysis to obtain an opls model with high predictive capability. this approach allowed to evaluate the composition of coffee blends of unknown arabica and robusta content, on the basis of multiple chemical components. differences in geographical origin of the a ...201223598112
transformation of coffee (coffea arabica l. cv. catimor) with the cry1ac gene by biolistic, without the use of markers.the transformation of coffee plantlets with the cry1ac gene of bacillus thuringiensis was achieved by biolistic using either the whole pubc plasmid or only the ubi-cry1ac-nos genetic cassette. the cry1ac gene was inserted into coffee plants in order to confer resistance to the leaf miner leucoptera coffeella, an insect responsible for considerable losses in coffee crops. bearing in mind that the genetic cassettes used for this study lack reporter genes and/or selection marker genes, the paramete ...201020549067
survey of philippine coffee beans for the presence of ochratoxigenic fungi.in 2012 to 2014, philippine green coffee beans from coffea arabica in benguet and ifugao; coffea canephora var. robusta in abra, cavite, and ifugao; and coffea liberica and coffea excelsea from cavite were collected and assessed for the distribution of fungi with the potential to produce ochratoxin a (ota). the presence of fungal species was evaluated both before and after surface sterilization. there were remarkable ecological and varietal differences in the population of ota-producing species ...201626814977
ochratoxigenic fungi associated with green coffee beans (coffea arabica l.) in conventional and organic cultivation in brazil.the genera aspergillus comprises species that produce mycotoxins such as aflatoxins, ochratoxins and patulin. these are cosmopolitan species, natural contaminants of agricultural products. in coffee grains, the most important aspergillus species in terms of the risk of presenting mycotoxins belong to the genera aspergillus section circumdati and section nigri. the purpose of this study was to assess the occurrence of isolated ochratoxigenic fungi of coffee grains from organic and conventional cu ...201324294225
effect of green coffea arabica l. seed oil on extracellular matrix components and water-channel expression in in vitro and ex vivo human skin models.green coffea arabica l. seed oil is being widely used in cosmetic formulations, although its effects on human skin cells are not clear and most observations are unpublished.200919250168
culture-dependent and -independent methods to investigate the predominant microorganisms associated with wet processed coffee.the fermentation process of yunnan arabica coffee is a typical wet fermentation. its excellent quality is closely related to microbes in the process of fermentation. the purpose of this study was to isolate and identify the microorganisms in the wet method of coffee processing in yunnan province, china. microbial community structure and dominant bacterial species were evaluated by traditional cultivated separation method and pcr-dgge technology, and were further analyzed in combination with the ...201627113591
safety studies on products from whole coffee fruit.the fruit of the coffee plant, coffea arabica, has high phenolic antioxidant and phytonutrient content and could be a beneficial food ingredient. however, the fruit has historically been discarded for the favored harvesting of the coffee bean alone. coffeeberry products are derived from the whole fruit and include a ground whole powder, a water extract, and a more recently developed water-ethanol extract. the safety of coffeeberry products was evaluated in three genotoxicity studies, three short ...201020600539
microbiological diversity associated with the spontaneous wet method of coffee fermentation.the evaluation of the microbiota present during coffee wet fermentation was done in two distinct regions of minas gerais, brazil: one farm in the south of minas gerais (lavras=l) and another farm in the savannah region (monte carmelo=mc). the yeast population ranged from 2.48 to 4.92 log cfu/g and from 2 to 4.81 log cfu/g, the mesophilic bacteria population ranged from 3.83 to 8.47 log cfu/g and from 5.37 to 7.36 log cfu/g, and the lab population ranged from 2.57 to 5.66 log cfu/g and from 3.40 ...201526119187
green synthesis of silver nanoparticles using coffea arabica seed extract and its antibacterial activity.a novel green source was opted to synthesize silver nanoparticles using dried roasted coffea arabica seed extract. bio-reduction of silver was complete when the mixture (agno3+extract) changed its color from light to dark brown. uv-vis spectroscopy result showed maximum adsorption at 459 nm, which represents the characteristic surface plasmon resonance of nanosilver. x-ray crystal analysis showed that the silver nanoparticles are highly crystalline and exhibit a cubic, face centered lattice with ...201626478284
heat stress causes alterations in the cell-wall polymers and anatomy of coffee leaves (coffea arabica l.).coffee plants were subjected to heat stress (37 °c) and compared with control plants (24 °c). cell wall polysaccharides were extracted using water (w), edta (e) and 4m naoh (h30 and h70). in addition, monolignols were analyzed, and the leaves were observed by microscopy. plants under heat stress accumulated higher contents of arabinose and galactose in fraction w. xylose contents were observed to decrease in h30 fractions after the heat stress, whereas galactose and uronic acid increased. h70 fr ...201223465912
plant nutrient partitioning in coffee infected with meloidogyne konaensis.two experiments were conducted to assess nutrient partitioning in coffee (coffea arabica cv. typica land race guatemala) infected with meloidogyne konaensis. nutrient levels were quantified from soil, roots, and leaves. in the first experiment, 500-cm3 aliquants of a kealakekua andisol were infested with four initial population densities of m. konaensis ranging from 0 to 1,500 freshly hatched second-stage juveniles. coffee plants (~3 months old) were transplanted into the soil and grown for 25 w ...200419262790
functional characterization of three coffea arabica l. monoterpene synthases: insights into the enzymatic machinery of coffee aroma.the chemical composition of the coffee beverage is extremely complex, being made up of hundreds of volatile and non-volatile compounds, many of which are generated in the thermal reactions that occur during the roasting process. however, in the raw coffee bean there are also compounds that survive roasting and are therefore extracted into the beverage. monoterpenes are an example of this category, as their presence has been reported in the coffee flower, fruit, seed, roasted bean and in the beve ...201323398891
cof a 1: identification, expression and immunoreactivity of the first coffee allergen.over the past years, dust of green coffee beans has become known to be a relevant cause for occupational type i allergies. up to now, allergy diagnostics is based on native green coffee bean extract which exhibits insufficient specificity due to interfering substances as well as batch-to-batch variations. no coffee allergen has been described on the molecular level so far. the aim of this study was to identify the first allergen of green coffee.201222722540
[coccus species affecting coffea arabica in brazil (coccoideae: coccidae) and the redescription of two species].five coccus on coffea arabica from the states of bahia, espírito santo and minas gerais were studied. females were prepared and mounted in canada balsam. coccus brasiliensis fonseca and coccus lizeri (fonseca) were redescribed and coccus alpinus de lotto, coccus celatus de lotto and coccus viridis (green) were characterized. a key to species and illustration of all of them are included. coccus alpinus and c. celatus are new records for brazil and the neotropical region.201020676513
synthesis of silver nanoparticles by endosymbiont pseudomonas fluorescens ca 417 and their bactericidal activity.the present study emphasizes on biogenic synthesis of silver nanoparticles and their bactericidal activity against human and phytopathogens. nanoparticle synthesis was performed using endosymbiont pseudomonas fluorescens ca 417 inhabiting coffea arabica l. synthesized nanoparticles were characterized using hyphenated spectroscopic techniques such as uv-vis spectroscopy which revealed maximum absorption 425nm. fourier transform infrared spectroscopy (ftir) analysis revealed the possible functiona ...201627866607
esterase polymorphism in meloidogyne konaensis.the continual detection of a slow (i1) esterase band in greenhouse cultures of meloidogyne konaensis isolated from the field led to a hypothesis that the nematode may be polymorphic for esterase. a survey of coffee fields demonstrated at least four esterase phenotypes were present in meloidogyne recovered. an f1 phenotype predominated (60% of the females), but an i1 phenotype was also common (30% of samples). a series of greenhouse and laboratory experiments were undertaken to understand this po ...200519262888
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