| fever and antipyresis in the bluegill sunfish, lepomis macrochirus. | | 1977 | 20274 |
| acute toxicity of dechlorinated ddt, chlordane and lindane to bluegill (lepomis macrochirus) and daphnia magna. | | 1979 | 88975 |
| in vitro metabolism of carbaryl by liver explants of bluegill, catfish, perch, goldfish, and kissing gourami. | | 1979 | 120882 |
| twenty-four hour records of body temperature and activity in bluegill sunfish (lepomis macrochirus) and brown bullheads (ictalurus nebulosus). | | 1975 | 236856 |
| hepatic microsomal epoxide hydratase of bluegill. | | 1979 | 486165 |
| excretion of 131i by freshwater catfish (ictalurus punctatus) and bluegill (lepomis macrochirus). | | 1979 | 489299 |
| predation, aggression, and activity levels in food-deprived sunfish (lepomis macrochirus and l. gibbosus): motivational interactions. | three experiments investigated the effects of food deprivation on several behavioral categories in two species of sunfish. in experiment 1, predatory behavior and general activity were observed under five levels of deprivation. for both species, predation measures increased in a similar negatively accelerating manner with increasing deprivation, while activity changed in a more complex fashion. experiment 2 examined the effects of deprivation on activity in a novel environment and showed that th ... | 1977 | 559695 |
| lepomis macrochirus rafinesque, a new host for argulus maculosus wilson. | | 1977 | 859092 |
| toxicity of intermittent chlorination to bluegill, lepomis macrochirus: interaction with temperature. | | 1977 | 861395 |
| oxygen and phosphate metabolism of bluegill liver mitochondria in the presence of some pesticides. | | 1975 | 1063679 |
| radioimmunoassay for salmon pancreatic somatostatin-25. | a specific and sensitive radioimmunoassay (ria) for the measurement of plasma levels of somatostatin-25 (ss-25) in salmon was developed using antisera raised against coho salmon (oncorhynchus kisutch) ss-25. somatostatin-25 was iodinated by the chloramine-t method and repurified on sephadex g-25. the ria was performed using a double antibody (goat anti-rabbit gammaglobulin as second antibody) method under disequilibrium conditions. plasma from several salmonids (coho, chinook, rainbow trout, bro ... | 1991 | 1676008 |
| spontaneously productive c-type retrovirus infection of fish cell lines. | the spontaneous production and release of morphologically typical, 85 to 90 nm diameter c-type retrovirus particles from four cell lines derived from three species of warmwater fish have been identified. virus pellets from cell culture supernatants showed high levels of mn(2+)-dependent reverse transcriptase activity at 24 degrees c. peak enzyme activity was associated with a 1 x 16 g/ml sucrose gradient fraction. all four isolates induced a cytolytic infection of a bluegill fry cell line within ... | 1991 | 1717644 |
| discovery of bone gamma-carboxyglutamic acid protein in mineralized scales. the abundance and structure of lepomis macrochirus bone gamma-carboxyglutamic acid protein. | the mineralized scale of the freshwater sunfish lepomis macrochirus (bluegill) contains a gla protein. the protein was identified in extracts of scale by a new colorimetric assay for gla-containing proteins. the protein was purified by gel filtration chromatography followed by reversed phase high performance liquid chromatography (hplc). several tests establish the identity of scale gla protein and bone gla protein (bgp). first, the proteins exhibit identical mobilities on electrophoresis and by ... | 1992 | 1597487 |
| selenium and other trace elements in bluegills from agricultural return flows in the san joaquin valley, california. | the effects of water quality and exposure to trace elements in irrigation return flows on bluegills (lepomis macrochirus) were studied in the merced river and salt slough, tributaries to the san joaquin river, california, during 1988. our study revealed that conductivity, turbidity, ph, total filterable residue, total hardness, and alkalinity were significantly greater in the salt slough, which receives tile drainage, then in the merced river, which does not receive tile drainage. the concentrat ... | 1992 | 1554258 |
| external biomarkers to assess chromium toxicity in adult lepomis macrochirus. | | 1992 | 1536994 |
| effects of the insecticides carbofuran and fenvalerate on adenylate parameters in bluegill sunfish (lepomis macrochirus). | bluegills (lepomis macrochirus) were exposed to two sublethal concentrations of carbofuran, a carbamate insecticide, and fenvalerate, a synthetic pyrethroid insecticide, in continuous flow bioassay systems for 30 days. adenylate parameters (atp concentration, total adenylates concentration, and adenylate energy charge) were monitored in gill, liver, muscle, and stomach tissues. there were significant decreases in adenylate energy charge of gill and stomach tissue relative to initial values after ... | 1991 | 1953023 |
| toxicity of chautauqua lake bridge runoff to young-of-the-year sunfish (lepomis macrochirus). | | 1990 | 2279143 |
| effect of malathion on the brain acetylcholinesterase activity of bluegill sunfish lepomis macrochirus. | | 1992 | 1498481 |
| a laboratory study of the toxicity of the butanol extract of endod (phytolacca dodecandra) on two species of freshwater fish and two species of aquatic snails. | the purpose of this study was to evaluate the toxicity of the butanol extract of endod (phytolacca dodecandra) on 4 species of aquatic animals. groups of 10 mosquito fish (gambusia affinis) and 8 bluegill (lepomis macrochirus) were exposed to the butanol extract of endod in 300 ml of water at concentrations of 0.0, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.2 or 2.0 ppm. groups of 10 tropical snails (biomphalaria glabrata) and 10 pond snails (physa spp) were also exposed to the crude extract in 5 ... | 1990 | 2353429 |
| effects of hepatotoxicants on the induction of microsomal monooxygenase activity in sunfish liver by beta-naphthoflavone and benzo[a]pyrene. | effects of chemically induced hepatic injury on biotransformation enzymes in fish were studied. sunfish hybrids (lepomis macrochirus x l. cyanellus) were dosed per os with allyl formate (alf) and carbon tetrachloride (ccl4), and the induction of liver erod (7-ethoxyresorufin o-deethylase) activity was subsequently challenged by injections of beta-naphthoflavone (bnf) and benzo[a]pyrene (b[a]p). hepatotoxicity of chemical treatments was assessed using blood enzymes (asat, alat, and ldh) along wit ... | 1992 | 1375151 |
| aquatic hazard assessment of the organophosphate insecticide fonofos. | this study determined the acute and chronic toxicity of the organophosphate insecticide fonofos to standard freshwater aquatic organisms under laboratory conditions. fonofos was acutely toxic to bluegill (lepomis macrochirus), daphnia (d. magna), and midge (chironomous riparius) at 5.3, 2.7, and 39 micrograms/l, respectively. three fonofos formulations (technical, 94.8% a.i.; 20g, field granular 20% a.i.; and 4e, field liquid 4#/gal a.i.) exhibited similar acute toxicities to bluegill. exposure ... | 1992 | 1375017 |
| brain acetylcholinesterase activity and optomotor behavior in bluegills, lepomis macrochirus exposed to different concentrations of diazinon. | organophosphorus pesticides inhibit acetylcholinesterase (ache) activity to varying degrees in different species of fish. estimation of ache activity is a useful tool for establishing the degree of pollution caused by these pesticides. behavioral changes are very good and sensitive indicators of any chemical pollution. changes in the optomotor behavior are easily quantifiable. therefore estimation of ache activity and changes in the optomotor response are taken as criteria to study the effects o ... | 1992 | 1282384 |
| effects of water hardness and salinity on the acute toxicity and uptake of fenvalerate by bluegill (lepomis macrochirus). | | 1989 | 2706346 |
| pilum pilum gen. et sp. n. (acanthocephala: echinorhynchidae) from freshwater fishes of the southeastern united states. | pilum pilum gen. et sp. n. is described from the warmouth, lepomis gulosus (cuvier); brown bullhead, ictalurus nebulosus (lesueur); yellow bullhead, i. natalis (lesueur); redbreast sunfish, l. auritus (linnaeus); bluegill, l. macrochirus rafinesque; spotted sunfish, l. punctatus (valenciennes); and redfin pickerel, esox americanus (gmelin), from the alabama river drainage, brown bullhead from the mobile bay drainage in alabama, and pirate perch, aphredoderus sayanus gilliams, from an atlantic co ... | 1976 | 1255353 |
| interaction of predation and intraspecific aggression in bluegill sunfish lepomis macrochirus. | | 1975 | 1239272 |
| diel activity rhythms and thermoregulatory behavior of bluegill in response to unnatural photoperiods. | | 1975 | 1169991 |
| changes in the darkness of four body features of bluegill sunfish (lepomis macrochirus rafinesque) during aggressive encounters. | | 1975 | 1169938 |
| susceptibility of bluegil sunfish (lepomis macrochirus) to nonionic surfactants. | the present report provides a basis for direct comparison of the susceptibility of fish to alkylphenol and alcohol ethoxylates of various eo chain lengths. it is obvious from the data provided that susceptibility of bluegil to nonionic surfactants (both types) increases with decreasing eo chain length. also it appears that bluegill are no more susceptible, and, in fact, are probably less susceptible to the acute effects of alkylphenol ethoxylates than of alcohol ethoxylates. | 1975 | 1125468 |
| circular dichroism of rhodopsins and porphyropsins. | circular dichroism and absorption spectra were determined for digitonin extracts of three rhodopsins: cattle, grass frog, and pigeon; and three porphyropsins: channel catfish, bluegill sunfish, and redear sunfish. a comparison of these spectra shows the following: (1) porphyropsins, like rhodopsins, exhibit two positive cd peaks in the spectral region 320--700 nm: an alpha peak at about 520 nm and a small beta peak at about 355 nm. these peaks substantially diminish upon bleaching. (2) in the cd ... | 1976 | 1083248 |
| the lethality of an intact and degraded las mixture to bluegill sunfish and a snail. | | 1976 | 988198 |
| social-aggressive behavior after various intervals of social isolation in bluegill sunfish (lepomis macrochirus rafinesque) in different states of reproductive readiness. | | 1976 | 986809 |
| locomotor hyperactivity induced in the bluegill sunfish, lepomis macrochirus, by sublethal concentrations of ddt. | | 1977 | 884635 |
| mercury levels in freshwater fish of the state of south carolina. | samples of fish from freshwater sources of rivers, lakes and ponds all over the state of south carolina were collected during the summer of 1974 and 1975. the fish collected were bass, bluegill, redbreast, catfish, shad, carp, crappie, mudfish and pike. samples were analyzed using the flameless atomic absorption procedure outlined by hatch and ott, and uthe et al as modified for use with perkin-elmer, coleman mas-50 mercury analyzer. triplicate samples of fish tissue were analyzed by wet digesti ... | 1977 | 836979 |
| uptake of channel catfish virus from water by channel catfish and bluegills. | radiolabeled channel catfish virus entered the gills of juvenile channel catfish (ictalurus punctatus) and was then concentrated in the gut and the liver over 48 hours. diminution of radioactivity was not detected in these tissues over the course of the experiment. bluegills (lepomis macrochirus) were capable of clearing the virus during a period of 48 hours. [3h]thymidine alone had a different distribution in the channel catfish than did labeled virus. | 1987 | 3032024 |
| radon and radium retention by the bluegill. | | 1979 | 478867 |
| acute toxicity of hydrogen cyanide to freshwater fishes. | acute toxicity of hydrogen cyanide was determined at various temperatures from 4 degrees to 30 degrees c and oxygen concentrations of 3.36 to 9.26 mg/l on different life history stages of five species of fish: fathead minnow, pimephales promelas refinesque; bluegill, lepomis macrochirus rafinesque; yellow perch, perca flavescens (mitchill); brook trout, salvelinus fontinalis (mitchill); and rainbow trout, salmo gairdneri richardson. median lethal threshold concentrations and 96-hr lc50's were es ... | 1978 | 215091 |
| transfer of 14c-toluene from mosquito larvae to bluegill sunfish. | | 1979 | 42456 |
| acute toxicity of selenium dioxide to freshwater fishes. | acute toxicity tests of selenium dioxide were conducted for 96 to 336 hr in intermittent-flow bioassay systems using six species of freshwater fish. the decreasing order of species sensitivity was: fathead minnow, flagfish, brook trout, channel catfish, goldfish, and bluegil. curves relating median lethal concentration to exposure time for each species exposed for more than 168 hr were sigmoid in shape and were characterized by a change in slope indicating a more rapid mortality rate after 96 to ... | 1976 | 5060 |
| trace element residues in bluegills and common carp from the lower san joaquin river, california, and its tributaries. | whole-body samples of bluegills (lepomis macrochirus) and common carp (cyprinus carpio) from the san joaquin river and two tributaries (merced river and salt slough) were analyzed to determine if the concentrations of any of nine elements were elevated as a result of exposure of the fish to agricultural subsurface (tile) drainage water. highest concentrations (micrograms/g dry weight) detected were as follows (first number in each pair applies to bluegills and the second to carp): arsenic (as), ... | 1988 | 3222692 |
| toxicity of natural pyrethrins and five pyrethroids to fish. | the toxicity of natural pyrethrins and five pyrethroids was determined with coho salmon (oncorhynchus kisutch), steelhead trout (salmo gairdneri), fathead minnow (pimephales promelas), channel catfish (icatlurus punctatus), bluegill (lepomis macrochirus), and yellow perch (perca flavescens). the 96-hour lc50's in static tests at 12 degrees c ranged from 24.6 to 114 mug/l of natural pyrethrins and from 0.110 to 1,140 mug/l of pyrethroids. two pyrethroids, ru-11679 and sbp-1382 (r), were over 10 t ... | 1976 | 5059 |
| the effects of exogenous 11-ketotestosterone, testosterone, and cyproterone acetate on prespawning and parental care behaviors of male bluegill. | this study investigated the effect of exogenous 11-ketotestosterone (11kt) and testosterone (t), two important androgens involved in fish reproduction, as well as cyproterone acetate (cya), an antiandrogen, on behaviors of male bluegill (lepomis macrochirus) during prespawning and parental care periods. parental male bluegill build nests in colonies, spawn with females, and then remain alone on these nests to provide care for their developing offspring. implantation of pellets containing either ... | 1991 | 1834540 |
| inhibition of atp dephosphorylation by acaricides with emphasis on the anti-atpase activity of the carbodiimide metabolite of diafenthiuron. | 3-(2,6-diisopropyl-4-phenoxyphenyl)-1-tert-butylcarbodiimide (dfcd), a toxic metabolite and photodegradation product of the propesticide diafenthiuron, and dicyclohexylcarbodiimide (dccd), a commonly used biochemical inhibitory probe, inhibited mg(2+)-, na+k(+)-, and ca2+mg(2+)-atpase activities in preparations from bulb mites (rhizoglyphus echinopus (fumouze and robin)), twospotted spider mites (tetranychus urticae koch), and bluegill (lepomis macrochirus rafinesque) brain. dccd was more active ... | 1991 | 1832178 |
| role of exposure mode in the bioavailability of triphenyl phosphate to aquatic organisms. | a laboratory study was conducted to investigate the role of the route of triphenyl phosphate (tpp) entry on its aquatic bioavailability and acute biological effects. three tpp treatments were used for exposures of fish and invertebrates. these consisted of tpp dosed directly into water with and without clean sediment and tpp spiked onto sediment prior to aqueous exposures. results of static acute toxicity tests (no sediment) were 0.78 mg/l (96-h lc50) for bluegill, 0.36 mg/l (48-h ec50) for midg ... | 1991 | 1759843 |
| dietary exposure of bluegills (lepomis macrochirus) to (75) se: uptake and distribution in organs and tissues. | | 1988 | 3382794 |
| visual responses of nucleus isthmi in a teleost fish (lepomis macrochirus). | extracellular electrical activity was recorded from the nucleus isthmi of the bluegill sunfish (lepomis macrochirus) in response to brief flashes produced by red light emitting diodes, and other visual stimuli. metal microelectrodes detected positive spikes outside the nucleus, and negative spikes inside. spikes of a continuous range of amplitudes up to 1 mv occurred in bursts, spontaneously and visually triggered. the highest amplitude spikes were triggered by the appearance or movement of stim ... | 1991 | 1843757 |
| purification of epizootic haematopoietic necrosis virus and its detection using elisa. | epizootic haematopoietic necrosis virus (ehnv) was grown in bluegill fry (bf-2) cells and purified using differential and gradient centrifugation. the lower band (b2) from 15-60% sucrose gradients contained infective ehnv but few contaminating cell components when assessed by electron microscopy, sds-page, and western blotting using anti-bf-2 serum and anti-b2 serum. both rabbit and sheep anti-b2 sera precipitated b2 in agarose gel immunodiffusion and detected ehnv in cell culture supernatant wh ... | 1991 | 1939507 |
| in vitro cyto- and genotoxicity of organomercurials to cells in culture. | the sequence of cytotoxic effects for a series of mercury compounds to the bg/f epithelioid cells derived from fin tissue of bluegill sunfish was phenyl mercuric chloride greater than methyl mercuric chloride greater than ethyl mercuric chloride much greater than mercuric chloride. this sequence of in vitro cytotoxicity was similar to that observed in a 48-h lc50 in vivo acute toxicity assay with rainbow trout. using induction of micronuclei as an indicator of genetic damage, the organomercurial ... | 1990 | 2309233 |
| effect of fenvalerate on metabolic ion dynamics in the fathead minnow (pimephales promelas) and bluegill (lepomis macrochirus). | | 1989 | 2545294 |
| mitochondrial and nuclear gene dynamics of introduced populations of lepomis macrochirus. | a population of bluegill sunfish, lepomis macrochirus, was introduced into three man-made ponds in 1966. analyses of these ponds in 1984 and 1985 found significant mtdna divergence without nuclear gene differentiation. the difference between nuclear gene and mtdna measures of interpopulational divergence was very large and suggests that sexual asymmetries in life histories may be important considerations in mtdna and nuclear gene rates of divergence. the rapid divergence of mtdna haplotype frequ ... | 1989 | 2583482 |
| toxicity of fenvalerate and its constituent isomers to the fathead minnow, pimephales promelas, and bluegill, lepomis macrochirus. | | 1987 | 3580587 |
| evolution of motor patterns: aquatic feeding in salamanders and ray-finned fishes. | patterns of muscle activity (motor patterns) have generally been found to be strongly conserved during the evolution of aquatic feeding behavior within closely related groups of fishes and salamanders. we conducted a test of the generality of motor pattern conservation with a much broader phylogenetic scope than has been done previously. activity patterns of three cranial muscles were quantified from electromyographic (emg) recordings made during suction feeding in a salamander (ambystoma mexica ... | 1989 | 2611639 |
| the intestinal loops of bluegill fish, lepomis macrochirus. | the structural configurations of the intestinal loops of bluegill fish (lepomis macrochirus) and a correlation of their probable functional significance have been investigated. the bluegill fish accommodates its relatively large intestine in its smaller body cavity by developing two intestinal loops. regional variations in the intestinal wall exist due to the presence of the intestinal loops. the structural complexity of the intestine by loop formation has apparently created a problem by posing ... | 1986 | 3751688 |
| effect of methylazoxymethanol acetate on bluegill sunfish cell cultures in vitro. | an epithelioid cell line derived from fin tissue of bluegill sunfish (designated bg/f) exhibited early indications of cell transformation upon exposure to methylazoxymethanol acetate (mam acetate). such changes included the induction of polyploidy, increased colony-forming efficiency, loss of contact inhibition, and formation of transformed foci. unlike later transformation characteristics observed with mammalian cells, the mam acetate-treated bg/f cells could not be propagated under conditions ... | 1989 | 2743918 |
| serum 11-ketotestosterone and testosterone concentrations associated with reproduction in male bluegill (lepomis macrochirus: centrarchidae). | male bluegill (lepomis macrochirus) display a complex reproductive behavior involving two alternative life history pathways: delay of sexual maturation to become "parentals" or precocious maturation as "cuckolders." the purpose of our study was to investigate the association of two androgens, 11-ketotestosterone (11kt) and testosterone (t), with reproduction in these two types of males. radioimmunoassay techniques were used to measure daily levels of the two androgens in the blood serum of paren ... | 1989 | 2792730 |
| species differences in 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and biotransformation in fish. | rainbow trout, yellow perch, carp, bluegill, largemouth bass, and bullhead were treated with graded doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd; 1, 5, 25, or 125 micrograms/kg) or vehicle, ip. the lethal potency of tcdd tended to be greater in yellow perch, carp, and bullhead than in the other three species (ld50 80 days post-treatment, 3-5 versus 10-16 micrograms/kg, respectively). all species treated with the highest dose of tcdd (125 micrograms/kg) displayed a latency period of 1-4 wee ... | 1988 | 3356307 |
| structure-activity relationships for diorganotins, chlorinated benzenes, and chlorinated anilines established with bluegill sunfish bf-2 cells. | the bluegill sunfish (lepomis macrochirus) bf-2 cell line, propagated at 34 degrees c, served as target for evaluation of the acute toxicities of various classes of aquatic pollutants, using the neutral red cytotoxicity assay. for a series of chlorinated benzenes and anilines, the sequence of cytotoxicity was dependent on the degree of chlorination and on their hydrophobicity, as described by their logarithmic octanol/water partition coefficients (log p values). with increasing numbers of chlori ... | 1988 | 3356316 |
| fathead minnow fhm cells for use in in vitro cytotoxicity assays of aquatic pollutants. | the suitability of the fathead minnow (fhm) epithelial cell line for use as the target (indicator) system in in vitro cytotoxicity assays was evaluated using several endpoints. the organometal diethyltin dichloride served as the representative test agent. the concentration of diethyltin dichloride which resulted in a midpoint toxicity was 3.5 microm in a 3-day cell growth assay, 3.8 microm in the 24-hr neutral red assay, and 16.5 microm in a 4-hr cell detachment assay. the neutral red assay was ... | 1987 | 3428209 |
| histopathological changes induced by malathion in the gills of bluegill lepomis macrochirus. | | 1989 | 2758128 |
| acid phosphatase activity in the intestine and caeca of bluegill, exposed to methyl mercuric chloride. | | 1986 | 3955260 |
| arylsulfatase and beta-glucuronidase expression in green sunfish, bluegill, and their reciprocal interspecific hybrids. | fish arylsulfatases (arylsulfate sulfohydrolase; ec 3.1.6.1) were resolved into cationic arylsulfatase a-like (arsa) and anionic arylsulfatase b-like (arsb) fractions by deae-sephacel chromatography. green sunfish (gsf) hepatic arsa was more acidic and more thermostable than bluegill (bg) arsa. gsf x bg interspecific hybrids preferentially expressed gsf arsa, while bg x gsf hybrids appeared to produce a dimeric enzyme consisting of both gsf and bg arsa polypeptides. gsf hepatic beta-glucuronidas ... | 1989 | 2775168 |
| sudden death by lepomis macrochirus (the killer fish). | in this unusual case, death was not caused by either anaphylactoid reaction to fish or by fish poisoning. death was due to asphyxia caused by a live, whole fish occluding the mouth and upper airway. | 1989 | 2782307 |
| comparative activity of gill atpase in three freshwater teleosts exposed to cadmium. | the comparative activity of gill atpase was examined in the bluegill sunfish (lepomis macrochirus), fathead minnow (pimephales promelas), and golden shiner (notemigonus crysoleucas). basal na/k atpase activity was highest in bluegill sunfish (1.46 mumol pi/mg protein/hr) and lowest in golden shiners (1.01 mumol pi/mg protein/hr). while a stimulation of na/k atpase activity was observed at an exposure concentration of 1 micrograms cd/liter in the bluegill sunfish and fathead minnows, an inhibitio ... | 1987 | 2826104 |
| the effects of temperature on the cytochrome p-450 system of thermally acclimated bluegill. | the effects of temperature acclimation at 10, 20 and 30 degrees c on the concentration and activity of the mixed function oxidase system in bluegill are as follows. liver weight/body weight varied inversely with temperature. significant (p less than 0.05) differences in concentration of cytochrome p-450 of hepatic microsomes were seen and varied inversely with temperature. benzo(a)pyrene hydroxylase activity tested in vitro at incubation temperatures of 10, 20 and 30 degrees c showed significant ... | 1985 | 2858333 |
| temperature compensation in the hepatic mixed-function oxidase system of bluegill. | bluegill (lepomis macrochirus r.) were acclimated to 12, 22 or 32 degrees c for 5 or 14 days. liver weight to body weight ratio and the rate of metabolism of benzo[alpha]pyrene by liver microsomes varied inversely with the acclimation temperature of the fish. concentration of microsomal cytochrome p-450, as determined by co-difference binding spectra, was not significantly affected by acclimation temperature. there were no qualitative or quantitative differences in the electrophoretic patterns o ... | 1985 | 2861037 |
| actions of cholecystokinin-related peptides on the gallbladder of bony fishes in vitro. | 1. cholecystokinin (cck) family peptides elicited dose-related gallbladder contractions in vitro for bluegill (lepomis macrochirus), killifish (fundulus heteroclitus), and bowfin (amia calva). 2. effects measured by increases in isometric contractions indicated equipotency for nonsulfated forms of cck and caerulein (crl; bluegill) and for sulfated cck and crl (bluegill, fundulus). sulfation increased potency. 3. responses to cck-related peptides were insensitive to atropine, a muscarinic antagon ... | 1988 | 2904870 |
| arsenic-selenium interactions determined with cultured fish cells. | a fibroblastic cell line derived from gill tissue (designated bg/g) and an epithelioid cell line derived from fin tissue (designed bg/f) of bluegill sunfish (lepomis macrochirus) were used as the bioindicators in toxicity experiments. the neutral red in vitro cytotoxicity assay served as the endpoint. in both cell lines the sequence of observed cytotoxicity was arsenite greater than arsenate greater than selenite greater than selenate, with each cell type exhibiting comparable ranking of midpoin ... | 1989 | 2919396 |
| [quantitative structure-activity relations of the lethal effects of 38 halogenated compounds against lepomis macrochirus]. | autocorrelation molecular descriptors have been used for the prediction of acute toxicity (96 hrs., lc 50) of 38 halogenated compounds on lepomis macrochirus (teleostei-centrarchidae). a stepwise regression procedure shows that structural and physico-chemical properties govern the ecotoxicological behaviour of these molecules. | 1986 | 3099980 |
| metabolism and dna adduct formation of benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene in fish cell lines in culture. | the metabolic activation of the carcinogens benzo[a]pyrene (bp) and 7,12-dimethylbenz[a]anthracene (dmba) was examined in cell lines derived from bluegill fry (bf-2), rainbow trout (rtg-2) and brown bullhead (bb). all three cell lines metabolized bp (0.5 microgram/ml medium) almost completely to water-soluble metabolites within 120 h, but the maximum amount of bp bound to dna ranged from only 5 pmol/mg dna in the bf-2 cells to 17 in the bb cells and 44 in the rtg-2 cells. the major bp-dna adduct ... | 1987 | 3115618 |
| the balances of 137 cs, stable cesium and potassium of bluegill (lepomis macrochirus raf.) and other fish in white oak lake. | | 1972 | 4642952 |
| seasonal abundance of gyrodactylus macrochiri hoffman and putz, 1964 on bluegill and largemouth bass. | | 1973 | 4701186 |
| the telencephalon and hypothalamus of the bluegill (lepomis macrochirus): evoked feeding, aggressive and reproductive behavior with representative frontal sections. | | 1971 | 4938346 |
| the cytological course of experimental lymphocystis in the bluegill. | | 1966 | 4959183 |
| ultrastructure of lymphocystis virus. | lymphocystis virus obtained from bluegills (lepomis macrochirus) was cultured in the permanent bluegill cell line bf-2 and examined by electron microscopy in ultrathin sections of cell cultures and in negative-contrast preparations from cells and from centrifuged culture medium. according to negative-contrast preparations, the icosahedral virions have an overall diameter close to but not exceeding 300 mmu. delicate filaments seem to issue from the vertices. in collapsed virions, an ordered array ... | 1968 | 4986903 |
| epitheliocystis, a new infectious disease of the bluegill (lepomis macrochirus). | | 1969 | 4987372 |
| seasonal abundance of ancyrocephalinaen (monogenoidea) parasites of bluegill, lepomis macrochirus (raf). | | 1972 | 5049118 |
| effects of cadmium, zinc, manganese, and calcium on oxygen and phosphate metabolism of bluegill liver mitochondria. | | 1971 | 5102849 |
| multiplication of lymphocystis virus in the bluegill (lepomis macrochirus). | | 1965 | 5220168 |
| comparative aspects of hepatic udp-glucuronosyltransferases and glutathione s-transferases in bluegill and channel catfish. | 1. microsomal udp-glucuronosyltransferases (udpgts) and cytosolic glutathione s-transferases (gsts) were examined in bluegill (lepomis macrochirus r.) and channel catfish (ictalurus punctatus r.) liver. 2. hepatic udpgt activity was of a similar magnitude in both species and was markedly increased by the addition of 0.05-0.2% triton x-100, however, optimal estimates of activity were obtained when 0.1% detergent was used. 3. both species exhibited hepatic gst activity toward several structurally ... | 1987 | 3117483 |
| spermiogenesis in the bluegill (lepomis macrochirus): a study of cytoplasmic events including cell volume changes and cytoplasmic elimination. | the process involved in the reduction of both nuclear and cytoplasmic volume was investigated in the bluegill (lepomis macrochirus), a teleost fish. young spermatids contained centrally positioned nuclei which, with time, moved toward the cell surface to become eccentrically positioned. chromatin condensation was initiated from a region near the implantation fossa, whereas at the opposite pole of the nucleus an area sparse in heterochromatin (clear area) was noted. the nuclear membrane lying adj ... | 1988 | 3216396 |
| the effect of temperature on the respiratory and cardiac response of the bluegill sunfish to hypoxia. | | 1969 | 5804484 |
| xenobiotic metabolizing enzyme systems in test fish. iii. comparative studies of liver cytosolic glutathione s-transferases. | the reduced glutathione (gsh)-conjugating capacities of the livers of trout, carp, zebra fish, guppy, and bluegill have been evaluated with several substrates preferentially metabolized by the major gsh-transferase isozymes. carp and zebra fish lack gsh-transferase activity with 1,2-epoxy-3-(p-nitrophenoxy)propane. guppy is characterized by the highest activities with 1-chloro-2,4-dinitrobenzene and the absence of activity toward trans-4-phenyl-3-buten-2-one. enzyme activities with the other sub ... | 1988 | 3234293 |
| cholecystokinin immunoreactivity in the digestive tract of bowfin (amia calva), bluegill (lepomis macrochirus), and bullfrog (rana catesbeiana). | the distribution of the intestinal hormone, cholecystokinin (cck), was studied in the gastrointestinal tract of a holostean fish, the bowfin (amia calva), and compared to a teleostean fish, the bluegill (lepomis macrochirus), and an amphibian, the bullfrog (rana catesbeiana), using an antiserum specific for the carboxyl terminal tetrapeptide of cck in an unlabeled biotin-avidin immunoperoxidase procedure. in the bowfin cck immunostained cells were detected only in the anterior and mid-intestine; ... | 1988 | 3286368 |
| xenobiotic-metabolizing enzyme systems in test fish. i. comparative studies of liver microsomal monooxygenases. | all monooxygenase activities assayed in guppy (poecilia reticulata) and red killifish (oryzias latipes) were 7-10 times higher than those measured in trout (salmo gairdneri), carp (cyprinus carpio), and golden orfe (leuciscus idus). cytochrome p-450 was 3 times higher in the former fish than in all the other species. zebra fish (brachydanio rerio) 7-ethoxycoumarin o-deethylase and bluegill (lepomis macrochirus) ethylmorphine n-demethylase (emnd) were not greatly different from those of the forme ... | 1987 | 3830019 |
| in vitro cytotoxicity of metals to bluegill (bf-2) cells. | | 1986 | 3947137 |
| effects of aroclor 1254 treatment on the in vitro hepatic metabolism of toluene, aniline and aminopyrine in hybrid sunfish. | the in vitro metabolism of the volatile aromatic hydrocarbon toluene by enzymes associated with the 12,000 g supernatant fraction of hybrid sunfish (lepomis macrochirus x l. cyanellus x l. gibbosus) liver homogenates was studied. aminopyrine demethylase (apdm) and aniline hydroxylase (ah) activities were measured. intramuscular injections of aroclor 1254 (a polychlorinated biphenyl) produced significant increases in apdm and ah activities (p less than or equal to 0.1, anova). there were no signi ... | 1984 | 6146461 |
| methylmercuric chloride and serum cholesterol level in the bluegill (lepomis macrochirus). | | 1986 | 3947753 |
| morphology and ultrastructure of lymphocystis disease virus, a fish iridovirus, grown in tissue culture. | the morphology and the ultrastructure of the lymphocystis disease virus (ldv) strain leetown , a fish iridovirus , was studied by electron microscopy. the virus was grown on bluegill fry (bf-2) cells at 21 degrees. ldv showed an icosahedral shape by ultrathin sections and negative staining, with a diameter of about 200 nm. the shell of the virion seemed to be composed of two unitary membranes with a total diameter of 16 nm. the outer membrane demonstrated swelling in negative staining, exhibitin ... | 1984 | 6203213 |
| decreased survival and teratogenesis during laboratory selenium exposures to bluegill, lepomis macrochirus. | | 1987 | 3440157 |
| in vitro effect of some mutagens/carcinogens on cultured fish cells. | fibroblast cells derived from juvenile bluegill sunfish were tested for their response the effects of known mutagens/carcinogens. the cells grew well and cloned in eagle's minimal essential medium which contained fetal bovine serum. growth rate was temperature dependent and increased steadily as temperature was raised from 15 to 33 degrees c. direct mutagens and promutagens were toxic to the cells, and fluorescence spectroscopy revealed the capability of metabolizing at least one promutagen, ben ... | 1981 | 6275801 |
| a comparative study of sertoli cell ectoplasmic specializations in selected non-mammalian vertebrates. | ectoplasmic specializations (es) facing spermatids were studied in species representative of four classes of non-mammalian vertebrates (pisces--bluegill; amphibia--bullfrog; reptilia--red eared turtle; aves--domestic chicken). es was not seen in the bluegill but was present in all other species studied. in the frog, turtle, and chicken, es did not resemble its mammalian counterpart and could only be characterized by the presence of 6 nm filaments (presumedly actin) within the somatic cell facing ... | 1987 | 3498237 |
| germ cell-somatic cell relationships: a comparative study of intercellular junctions during spermatogenesis in selected non-mammalian vertebrates. | specialized germ cell-somatic cell relationships were surveyed in the testis of species representative of four classes of non-mammalian vertebrates. desmosome-like junctions were present in all classes studied. in the teleost fish studied (bluegill; lepomis macrochirus), small, infrequent desmosomes, seen between the spherical cyst cells and spermatocytes, were characterized by poorly represented subsurface densities. in the bullfrog (rana catesbeiana), similar desmosome-like junctions were foun ... | 1987 | 3498985 |
| polycyclic aromatic hydrocarbon in vitro cytotoxicity to bluegill bf-2 cells: mediation by s-9 microsomal fraction and temperature. | the in vitro cytotoxicities of various polycyclic aromatic hydrocarbons (pahs) to bluegill sunfish bf-2 cells were determined with the neutral red assay, which was modified by the incorporation of an s-9 microsomal fraction. whereas the pahs per se were weakly cytotoxic, the presence of the s-9 fraction in the incubation mixture increased the cytotoxicity of many of the pahs, presumably due to the formation of active metabolites. in addition, the cytotoxicity of the pahs (whether in the absence ... | 1987 | 3576641 |
| bioaccumulation factor for 32p measured in bluegill, lepomis macrochirus, and catfish, ictalurus punctatus. | the ratio of the bioaccumulation factors for 32p and phosphorus was determined for edible tissue in two species of freshwater fish by measuring the specific activity (32p activity per milligram phosphorus) in muscle relative to feed. the 32p tracer was added to the feed at a uniform level throughout the study. feeding was at two levels: ad libitum and at a lower but constant intake per body weight. in the main experiment, bluegill were maintained in a large flow-through tank and sacrificed at ap ... | 1987 | 3654227 |
| idiopathic skeletal muscle necrosis in bluegills. | skeletal muscle degeneration and necrosis in a wild population of bluegills (lepomis macrochirus) were investigated. hemorrhage and large areas of muscle necrosis were evident at necropsy. histologically, muscle fibers were granular, vacuolated, and fragmented. ultrastructural alterations included mitochondrial swelling, clumping and loss of actin and myosin fibrils, swelling of t tubules, and loss of continuity of the sarcolemma. an etiologic agent was not identified. | 1984 | 6511585 |
| toxicity of selected priority pollutants to various aquatic organisms. | toxicity tests were conducted with selected compounds listed by the united states environmental protection agency (epa) as priority pollutants. acute toxicity information was determined for acenaphthene, arsenic trioxide, cadmium chloride, mercury(ii) chloride, silver nitrate, chlordane, endosulfan, and heptachlor. acute tests were conducted using one or more of the following species: fathead minnows (pimephales promelas), channel catfish (ictalurus punctatus), rainbow trout (salmo gairdneri), b ... | 1983 | 6617566 |
| separation by ion-pair high-performance liquid chromatography of the glucuronide, sulfate and glutathione conjugates formed from benzo[a]pyrene in cell cultures from rodents, fish and humans. | a large proportion of the metabolites formed from benzo[a]pyrene (bp) in cell cultures from rodents, fish and humans result from conjugation of an oxidized metabolite of bp with sulfate, glucuronic acid or glutathione (gsh). to improve the analysis of these metabolites, a reversed-phase ion-pair h.p.l.c. system using a step gradient of methanol:tetrabutyl-ammonium bromide in ammonium formate buffer has been developed for the separation of these three classes of conjugates. this system separated ... | 1987 | 3802396 |
| in vitro cytotoxicity of organic pollutants to bluegill sunfish (bf-2) cells. | bf-2 cells, an established cell line derived from bluegill sunfish, (lepomis macrochirus), were exposed to 18 organic toxicants, with cytotoxicity being assayed by the neutral red (nr) technique. based on the concentration of toxicant that reduced lysosomal uptake of neutral red by 50% (nr50), the rank order of cytotoxicity was methyl mercury greater than pentachlorophenol greater than 2,3,5,6-tetrachlorophenol greater than 2,3,5-trichlorophenol greater than 2,3-dinitrotoluene greater than 2,4,6 ... | 1987 | 3803339 |
| the histological and biochemical effects of cadmium exposure in the bluegill sunfish (lepomis macrochirus). | cadmium effects on the bluegill sunfish (lepomis macrochirus) were assessed histologically and biochemically and the effects were compared with effects on the ecologically relevant parameters of growth and survival. growth and survival were monitored and tissues were removed for histopathological assessment of toxicant effects in a 163-day chronic exposure. the biochemical effects of cadmium were determined in a 32-day subchronic exposure. exposure of fish to cadmium in hard water (363 mg cd/lit ... | 1986 | 3956430 |
| in vitro cytotoxicity testing of aquatic pollutants (cadmium, copper, zinc, nickel) using established fish cell lines. | the cytotoxicity of cadmium toward cultured bluegill fry (bf-2) cells was determined using several assay endpoints. the concentrations of cadmium causing a 50% decrease in colony formation, cell replication, uptake of neutral red, population growth (as determined by protein analysis), and uptake of [3h]uridine and 50% detachment of cells (as determined by protein analysis) were 0.03, 0.04, 0.08, 0.09, 0.12, and 0.21 mm cadmium, respectively. the neutral red assay was used to compare the relative ... | 1986 | 3956434 |
| toxicity of adjuvants to bluegill. | | 1985 | 3971055 |