| histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: atlantic croaker (micropogonias undulatus), spotted seatrout (cynoscion nebulosus), and red drum (sciaenops ocellatus). | the adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. in general greater specificity for the different cell types was observed with antisera to the piscine pituitary hormones than with antisera raised against the human hormones. the distribution of the cell types did not differ significantly among the three species, a ... | 1991 | 1667000 |
| conserved restriction sites within the ribosomal rna genes of vertebrates. | we have mapped the cleavage sites of four restriction enzymes which recognize six-base sequences within the nuclear ribosomal (rrna) genes of twelve vertebrates, including several placental mammals (homo sapiens, man; bos taurus, cow; equus caballus, horse; sus scofra, pig; ovis aries, sheep; rattus rattus, rat), a marsupial (didelphis marsupialis, opossum), a bird (gallus domesticus, chicken), an amphibian (xenopus laevis), a reptile (alligator mississipiensis), a bony fish (cynoscion nebulosus ... | 1986 | 3004584 |
| mechanism of stimulatory action of growth hormone on ovarian steroidogenesis in spotted seatrout, cynoscion nebulosus. | the mechanism of growth hormone's (bovine growth hormone, bgh) stimulatory action on steroidogenesis was examined using ovarian tissue of spotted seatrout (cynoscion nebulosus) in an in vitro incubation system and compared to that of a gonadotropin (human chorionic gonadotropin, hcg). testosterone and estradiol production by seatrout ovaries in vitro were stimulated even with low concentrations of bgh (50 ng/ml), and increasing concentrations demonstrated a concentration-dependent relationship s ... | 1993 | 8392959 |
| human homologs of the putative g protein-coupled membrane progestin receptors (mpralpha, beta, and gamma) localize to the endoplasmic reticulum and are not activated by progesterone. | the steroid hormone progesterone exerts pleiotrophic functions in many cell types. although progesterone controls transcriptional activation through binding to its nuclear receptors, it also initiates rapid nongenomic signaling events. recently, three putative membrane progestin receptors (mpralpha, beta, and gamma) with structural similarity to g protein-coupled receptors have been identified. these mpr isoforms are expressed in a tissue-specific manner and belong to the larger, highly conserve ... | 2006 | 16959873 |
| acoustic pressure and particle motion thresholds in six sciaenid fishes. | sciaenid fishes are important models of fish sound production, but investigations into their auditory abilities are limited to acoustic pressure measurements on five species. in this study, we used auditory brainstem response (abr) to assess the pressure and particle acceleration thresholds of six sciaenid fishes commonly found in chesapeake bay, eastern usa: weakfish (cynoscion regalis), spotted seatrout (cynoscion nebulosus), atlantic croaker (micropogonias undulatus), red drum (sciaenops ocel ... | 2008 | 18424685 |
| poecilancistrium caryophyllum and other trypanophynch cestode plerocercoids from the musculature of cynoscion nebulosus and other sciaenid fishes in the gulf of mexico. | examination of over 3,000 fish, all sciaenids, has shown that plerocercoids of poecilancistrium caryophyllum infect cynoscion nebulosus, bairdiella chrysura, sciaenops ocellata, c. arenarius, micropogonias undulatus, and pogonias cromis in mississippi sound. in addition to p. caryophyllum, a pseudophyllidean-like trypanorhynch also infected c. nebulosus and m. undulatus. pseudogrillotia pleistacantha selectively infected large p. cromis, pterobothrium heteracanthum infected m. undulatus, and pte ... | 1977 | 915606 |
| habitat-related predation on juvenile wild-caught and hatchery-reared red drum sciaenops ocellatus (linnaeus). | we examined the patterns of habitat-specific mortality for newly settled red drum (sciaenops ocellatus) using an experimental mesocosm approach. experiments were designed to analyze prey vulnerability and fish rearing-type (wild-caught or hatchery-reared) in estuarine habitats of varying structural complexity including marsh (spartina alterniflora loisel), oyster reef (crassostrea virginica gmelin), seagrass (halodule wrightii aschers), and nonvegetated sand bottom. we used two different predato ... | 2001 | 11358569 |
| kudoa inornata sp. n. (myxosporea: multivalvulida) from the skeletal muscles of cynoscion nebulosus (teleostei: sciaenidae). | a new myxosporean species, kudoa inornata sp. n. (myxosporea: multivalvulida), is described from skeletal muscles of the spotted seatrout cynoscion nebulosus (cuvier), collected in estuarine waters along the coast of south carolina, usa. light microscopic and ultrastructural characters rank this species to the group of kudoa species with simple-shaped spores. the uniqueness of the ssu and lsu rdna sequences justifies its status of a new species with sister relationship to kudoa paniformis. the 1 ... | 2009 | 19606785 |
| evaluation of phosphorus, protein, and n-3 fatty-acid content in 15 marine fish species identifies the species most beneficial to renal patients. | among 15 marine fish species, we sought to identify those most beneficial to renal patients by evaluating their phosphorus (p), protein (pc), and n-3 fatty-acid (n-3 pufa) content. | 2009 | 19818298 |
| mycobacteria isolated from chesapeake bay fish. | mycobacteriosis in fish can result in ulcers, emaciation, and in some cases death. mycobacteria have been previously isolated from a variety of chesapeake bay fish species, and the current study was designed to identify potential host specificity and location fidelity of mycobacterial isolates. mycobacteria were isolated from wild fish of the chesapeake bay collected from the upper bay, the choptank river, herring bay, the chicamacomico river, the pocomoke river and the potomac river in 2003-200 ... | 2010 | 19909394 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| use of molecular tools in identification of philometrid larvae in fishes: technical limitations parallel our poor assessment of their biodiversity. | the usefulness of the polymerase chain reaction with restriction fragment length polymorphism (pcr-rflp) and partial sequencing of the cytochrome oxidase i (coi) gene was tested regarding the utility of these techniques in unraveling philometrid life cycles and, in particular, to determine putative paratenic host species. our focus was to study three species of philometrids commonly found in the estuaries of south carolina: philometroides paralichthydis and philometra overstreeti from the southe ... | 2011 | 21656267 |
| stable isotopes and mercury in a model estuarine fish: multibasin comparisons with water quality, community structure, and available prey base. | stable-isotope ratios (δ(13)c and δ(15)n) and mercury in a model predator, and associated prey community assessments were used to make inferences regarding food web relationships and how these relationships are influenced by habitat variability and anthropogenic factors. although interconnected, the three major basins of the indian river lagoon system on the atlantic coast of florida comprise noticeably different available habitat types with spatially distinct faunal communities and available pr ... | 2011 | 22104385 |
| oyster reefs as natural breakwaters mitigate shoreline loss and facilitate fisheries. | shorelines at the interface of marine, estuarine and terrestrial biomes are among the most degraded and threatened habitats in the coastal zone because of their sensitivity to sea level rise, storms and increased human utilization. previous efforts to protect shorelines have largely involved constructing bulkheads and seawalls which can detrimentally affect nearshore habitats. recently, efforts have shifted towards "living shoreline" approaches that include biogenic breakwater reefs. our study e ... | 2011 | 21850223 |
| variation in fatty acid composition among nine forage species from a southeastern us estuarine and nearshore coastal ecosystem. | the fatty acid (fa) composition of nine potentially important forage species was determined (n = 330): red drum (sciaenops ocellatus), spot (leiostomus xanthurus), spotted seatrout (cynoscion nebulosus), striped mullet (mugil cephalus), pinfish (lagodon rhomboides), atlantic croaker (micropogonias undulatus), star drum (stellifer lanceolatus), striped anchovy (anchoa hepsetus), and brief squid (lolliguncula brevis). samples were collected from estuarine and nearshore coastal waters around charle ... | 2008 | 18665465 |
| sciaenid inner ears: a study in diversity. | sciaenid fishes (family sciaenidae) could potentially serve as models for understanding the relationship between structure and function in the teleost auditory system, as they show a broad range of variation in not only the structure of the ear but also in the relationship between the ear and swim bladder. in this study, scanning electron microscopy (sem) was used to investigate inner ear ultrastructure of the atlantic croaker (micropogonias undulatus), spotted seatrout (cynoscion nebulosus), ki ... | 2001 | 11910172 |
| factors affecting toxicity test endpoints in sensitive life stages of native gulf of mexico species. | indigenous species are less commonly used in laboratory aquatic toxicity tests compared with standard test species due to (1) limited availability lack of requisite information necessary for their acclimation and maintenance under laboratory conditions and (2) lack of information on their sensitivity and the reproducibility of toxicity test results. as part of the natural resource damage assessment aquatic toxicity program in response to the deepwater horizon oil incident (2010), sensitive life ... | 2015 | 25563746 |
| acoustic monitoring indicates a correlation between calling and spawning in captive spotted seatrout (cynoscion nebulosus). | fish sound production is widespread throughout many families. territorial displays and courtship are the most common reasons for fish sound production. yet, there is still some questions on how acoustic signaling and reproduction are correlated in many sound-producing species. in the present study, our aim was to determine if a quantitative relationship exists between calling and egg deposition in captive spotted seatrout (cynoscion nebulosus). this type of data is essential if passive acoustics ... | 2017 | 28289557 |
| effects of release procedures on the primary stress response and post-release survival and growth of hatchery-reared spotted seatrout cynoscion nebulosus. | to help explain the apparent poor post-release success of hatchery-reared (hr) spotted seatrout cynoscion nebulosus, this study examined the effects of handling, transport and release procedures on the stress response of two age classes [48 and 80 day post-hatch (dph)] of hr c. nebulosus, as measured by cortisol concentrations and the post-release survival and growth of 48 and 80 dph hr c. nebulosus. as a proxy for stress, tissue cortisol was measured at various times during the handling, taggin ... | 2017 | 27905106 |
| quantifying the seafood consumption patterns of recreational anglers in charleston and berkeley counties, south carolina. | this study was designed to provide self-reported data on the frequency of fish consumption and shellfish consumption in charleston and berkeley (cb) counties, south carolina. while commercial fishing and recreational fishing have played an important role in the culture and history of the area, information on the specific patterns of consumption by recreational anglers has been previously unavailable. the pilot data presented here will help determine the feasibility of a large-scale survey of sea ... | 2016 | 27891049 |
| co-exposure to sunlight enhances the toxicity of naturally weathered deepwater horizon oil to early lifestage red drum (sciaenops ocellatus) and speckled seatrout (cynoscion nebulosus). | the 2010 deepwater horizon oil spill resulted in the accidental release of millions of barrels of crude oil into the gulf of mexico. photo-induced toxicity following co-exposure to ultraviolet (uv) radiation is 1 mechanism by which polycyclic aromatic hydrocarbons (pahs) from oil spills may exert toxicity. red drum and speckled seatrout are both important fishery resources in the gulf of mexico. they spawn near-shore and produce positively buoyant embryos that hatch into larvae in approximately ... | 2017 | 27868239 |
| comparative ontogeny of the feeding apparatus of sympatric drums (perciformes: sciaenidae) in the chesapeake bay. | the anatomy of the feeding apparatus in fishes, including both oral and pharyngeal jaw elements, is closely related to the ecology of a species. during ontogeny, the oral and pharyngeal jaws undergo dramatic changes. to better understand how such ontogenetic changes occur and relate to the feeding ecology of a species, ontogenetic series of four closely related members of the family sciaenidae (cynoscion nebulosus, cynoscion regalis, micropogonias undulatus, and leiostomus xanthurus) were examin ... | 2016 | 26558606 |
| development of a modified cortisol extraction procedure for intermediately sized fish not amenable to whole-body or plasma extraction methods. | the corticosteroid hormone cortisol is the central mediator of the teleost stress response. therefore, the accurate quantification of cortisol in teleost fishes is a vital tool for addressing fundamental questions about an animal's physiological response to environmental stressors. conventional steroid extraction methods using plasma or whole-body homogenates, however, are inefficient within an intermediate size range of fish that are too small for phlebotomy and too large for whole-body steroid ... | 2016 | 26245954 |
| vitrification of sperm from marine fishes: effect on motility and membrane integrity. | our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. the objectives were to: 1) estimate acute toxicity of cryoprotectants over a range of concentrations; 2) evaluate the properties of vitrification solutions (vs); 3) evaluate different thawing solutions, and 4) evaluate sperm quality after thawing by examination of motility and membrane integrity. sperm were collected from red snapper (lutjanus campechanus), s ... | 2015 | 26074721 |
| the muscle dwelling myxozoan, kudoa inornata, enhances swimming performance in the spotted seatrout, cynoscion nebulosus. | parasites usurp host resources and, as a consequence, enhance their transmission and increase their fitness while reducing the fitness of their host. performance capacity is a key predictor of fitness. thus, the effects of parasites on host fitness may often be mediated by alteration of host performance. we tested the effect of the skeletal muscle dwelling myxozoan, kudoa inornata, on the swimming performance in spotted seatrout, cynoscion nebulosus. we predicted greater infection would result i ... | 2015 | 25876046 |
| genetic population structure of spotted seatrout cynoscion nebulosus along the south-eastern u.s.a. | analyses of the genetic population structure of spotted seatrout cynoscion nebulosus along the south-eastern u.s. coast using 13 microsatellites suggest significant population differentiation between fish in north carolina (nc) compared with south carolina (sc) and georgia (ga), with new river, nc, serving as an area of integration between northern and southern c. nebulosus. although there is a significant break in gene flow between these areas, the overall pattern throughout the sampling range ... | 2014 | 24890601 |
| sinuolinea infections in the urinary system of cynoscion species (sciaenidae) and phylogenetic position of the type species of sinuolinea davis, 1917 (myxozoa: myxosporea). | myxosporean infections that we diagnosed frequently in the urinary tract of cynoscion nebulosus (cuvier, 1830) and cynoscion regalis (bloch and schneider, 1801) (sciaenidae) collected in the estuarine systems of sc, usa, are described together with their etiological agent. based on the morphology of spores and plasmodial stages, we identified the agent, in both fish host species, as sinuolinea dimorpha (davis, 1916), which is the type species of the genus. based on sequences of ssu rdna generate ... | 2013 | 24533311 |
| gene expression and growth as indicators of effects of the bp deepwater horizon oil spill on spotted seatrout (cynoscion nebulosus). | the bp deepwater horizon oil spill has great potential to negatively affect estuarine fish populations. in order to assess possible impacts of this event, a series of sublethal lab experiments were performed, using the economically and ecologically important species spotted seatrout (cynoscion nebulosus). larval and juvenile spotted seatrout were exposed to sublethal concentrations of high energy water accommodated fraction (hewaf), chemically enhanced water accommodated fraction (cewaf), or dis ... | 2013 | 24283371 |
| population model for amyloodinium ocellatum infecting the spotted seatrout cynoscion nebulosus and the red snapper lutjanus campechanus. | the dinoflagellate amyloodinium ocellatum, a major pathogen in warm water mariculture, has a trophont, a tomont and a dinospore life history stage. this paper presents a population model for a. ocellatum infecting spotted seatrout cynoscion nebulosus and red snapper lutjanus campechanus and evaluates the relative effect of each vital rate on the a. ocellatum population growth rate. the vital rates were estimated by incubating trophonts in vitro and tracking their development through the successi ... | 2013 | 24113247 |
| monogenoideans (platyhelminthes) from the gill lamellae of the spotted sea trout, cynoscion nebulosus (perciformes, sciaenidae), from the western coast of the yucatan peninsula, mexico, with redescription of diplectanum bilobatus hargis 1955 (diplectanidae). | the gill lamellae ectoparasites of the spotted sea trout, cynoscion nebulosus (sciaenidae), in the western coast of the yucatán peninsula, mexico, revealed three species of monogenoidea: cynoscionicola heteracantha (manter 1938) price, 1962 (microcotylidae); choricotyle cynoscioni (maccallum, 1917) llewellyn, 1941 (diclidophoridae); and diplectanum bilobatus hargis, 1955 (diplectanidae). brief comments about the current taxonomic status as well as supplemental observations of all these monogenoi ... | 2013 | 23686533 |
| diversity of the skin microbiota of fishes: evidence for host species specificity. | skin microbiota of gulf of mexico fishes were investigated by ribosomal internal spacer analysis (risa) and 16s rrna gene sequencing. a total of 102 fish specimens representing six species (mugil cephalus, lutjanus campechanus, cynoscion nebulosus, cynoscion arenarius, micropogonias undulatus, and lagodon rhomboides) were sampled at regular intervals throughout a year. the skin microbiota from each individual fish was analyzed by risa and produced complex profiles with 23 bands on average. simil ... | 2013 | 23607777 |
| feeding performance of juvenile hatchery-reared spotted seatrout cynoscion nebulosus. | the feeding performance of individual hatchery-reared (hr) and wild juvenile spotted seatrout cynoscion nebulosus was compared across a series of six 1·5 h feeding exposures over a 3 day period in a controlled experiment. the predation cycle served as a context for discerning feeding performance elements. the experimental design facilitated assessments of the effects of experience, motivation due to hunger or satiation and prey density and encounter frequency. although feeding success improved s ... | 2013 | 23464558 |
| henneguya cynoscioni sp. n. (myxosporea: bivalvulida), an agent of severe cardiac lesions in the spotted seatrout, cynoscion nebulosus (teleostei: sciaenidae). | a new myxosporean species, henneguya cynoscioni sp. n., is described from the spotted seatrout, cynoscion nebulosus (cuvier) (sciaenidae) as a causative agent of cardiac henneguyosis. this new myxosporean species is characterized by the morphology of spores and the sequence of ssu rdna. examination of 227 spotted seatrout from four south carolina estuaries in 2008-2010 revealed a 33.5% total prevalence of h. cynoscioni. henneguya cynoscioni produces lesions in the bulbus arteriosus, its specific ... | 2011 | 22053614 |
| susceptibility and tolerance of spotted seatrout, cynoscion nebulosus , and red snapper, lutjanus campechanus , to experimental infections with amyloodinium ocellatum. | amyloodinium ocellatum is a parasitic dinoflagellate that infects warm-water marine and estuarine fishes and causes mortalities in aquaculture. its life cycle consists of 3 stages: a feeding trophont that parasitizes the gills and skin where it interferes with gas exchange, osmoregulation, and tissue integrity; a detached reproductive tomont; and a free-swimming infective dinospore. we compared the susceptibility and tolerance of juvenile spotted seatrout, cynoscion nebulosus, and red snapper, l ... | 2011 | 21506818 |
| mercury contamination in spotted seatrout, cynoscion nebulosus: an assessment of liver, kidney, blood, and nervous system health. | marine fishes in south florida (florida keys-florida bay-everglades region) accumulate higher concentrations of mercury (hg) in their tissues than similar fishes from other areas of the southeastern u.s., though it is not known whether these elevated levels affect fish health. in this study, we used quantifiable pathological and biochemical indicators to explore hg-associated differences in marine fish from south florida, where hg contamination is high, and from indian river lagoon, florida, whi ... | 2010 | 20850170 |
| synchronization of occurrence of the ovarian philometrid, philometra carolinensis, with the spawning season of its fish host, the spotted seatrout, cynoscion nebulosus. | the philometrid philometra carolinensis inhabits the ovaries of the spotted seatrout, cynoscion nebulosus. a 2-year study in estuaries of south carolina showed that each year adult female worms were present only during the spawning season of the host and that only sexually mature fish were infected. overall prevalence was 13.1%. young-of-the-year fish were uninfected and mature 1-year-old fish were less frequently infected than older fish. abundance of the philometrid was significantly different ... | 2009 | 19093133 |
| two new species of philometra (nematoda: philometridae) parasitic in the perciform fish cynoscion nebulosus (sciaenidae) in the estuaries of south carolina, usa. | two new nematode species of philometra costa, 1845, p. carolinensis sp. n. and p. cynoscionis sp. n., are described from the spotted seatrout, cynoscion nebulosus (cuvier) (sciaenidae, perciformes) in estuaries on the atlantic coast of south carolina, usa. philometra carolinensis (males and gravid and subgravid females) parasitic in the host's ovary is most similar to p. lateolabracis (yamaguti, 1935) in female morphology, but distinctly differs from it in possessing conspicuously short spicules ... | 2006 | 16696433 |
| chemical interference with genomic and nongenomic actions of steroids in fishes: role of receptor binding. | the characteristics of steroid nuclear and membrane receptors and their interactions with xenobiotic chemicals in two marine perciform species, atlantic croaker (micropogonias undulatus) and spotted seatrout (cynoscion nebulosus) are briefly reviewed. several organochlorines that bind to the nuclear progesterone receptor in mammals show negligible binding to the nuclear progestogen receptor in seatrout ovaries. two distinct nuclear androgen receptors with different tissue distributions have been ... | 2007 | 11460678 |
| upregulation of the maturation-inducing steroid membrane receptor in spotted seatrout ovaries by gonadotropin during oocyte maturation and its physiological significance. | changes in ovarian maturation-inducing steroid (mis; 17,20 beta, 21-trihydroxy-4-pregnen-3-one [20 beta-s]) membrane receptor concentrations during the reproductive cycle were investigated in spotted seatrout (cynoscion nebulosus) captured at their spawning grounds. ovarian receptor concentrations increased gradually during ovarian recrudescence and subsequently increased rapidly during oocyte maturation, reaching 3.5-fold the prematuration values by the beginning of ovulation. the significant e ... | 2001 | 11133654 |
| induction of ovulation of mature oocytes by the maturation-inducing steroid 17,20beta,21-trihydroxy-4-pregnen-3-one in the spotted seatrout. | incubation of mature, hydrated, follicle-enclosed oocytes of the spotted seatrout, cynoscion nebulosus, with the maturation-inducing steroid (mis), 17,20beta,21-trihydroxy-4-pregnen-3-one (20beta-s), for 9-12 h resulted in the appearance of ovulated oocytes in the culture media. the ovulation response was concentration-dependent and steroid-specific. the other teleost mis, 17, 20beta-dihydroxy-4-pregnen-3-one (17,20beta-p), was also a potent inducer of ovulation, whereas progesterone and 11-deox ... | 1999 | 10417233 |
| pesticides interfere with the nongenomic action of a progestogen on meiotic maturation by binding to its plasma membrane receptor on fish oocytes. | although many environmental contaminants disrupt endocrine function by binding to nuclear steroid receptors, it is not known whether they are capable of binding to steroid membrane receptors and interfering with nongenomic actions of steroids. the binding of several organochlorine pesticides to the plasma membrane receptor for the maturation-inducing steroid, 17,20beta,21-trihydroxy-4-pregnen-3-one (20beta-s), in the ovaries of spotted seatrout (cynoscion nebulosus) was investigated in in vitro ... | 1999 | 10098537 |
| sensory development and its relation to habitat change in three species of sciaenids. | visual and mechanosensory development of three sciaenid species was investigated to examine possible correlations between sensory morphology and patterns of habitat use. although the three species have different migration patterns as early larvae, few differences in sensory morphology occurred between species until late in the larval period. atlantic croaker, micropogonias undulatus, were distinguished by enhancements of visual morphology (large eyes, abundant photoreceptors, and best summation ... | 1998 | 9807012 |
| correlation between binding affinities of c21 steroids for the maturation-inducing steroid membrane receptor in spotted seatrout ovaries and their agonist and antagonist activities in an oocyte maturation bioassay. | the relative binding affinities of steroids for the maturation-inducing steroid (mis) plasma membrane receptor in spotted seatrout (cynoscion nebulosus) ovaries were compared to their relative potencies in inducing final oocyte maturation (fom) of seatrout oocytes in vitro. the mis receptor is specific for c21 steroids (pregnenes) lacking ketone or hydroxyl (oh) groups at the 11 position. the addition of single oh groups at the 17, 20, or 21 positions and two ohs at the 17, 20 and at the 17, 21 ... | 1997 | 9369163 |
| changes in plasma sex steroid-binding protein levels associated with ovarian recrudescence in the spotted seatrout (cynoscion nebulosus). | adult spotted seatrout were sampled from the wild during the period of their yearly gonadal recrudescence to study the association of plasma sex steroid-binding protein (sbp) levels with seasonal reproductive development. plasma sbp levels were estimated by scatchard analysis of the binding of radiolabeled testosterone to seatrout plasma. plasma sbp levels were lowest in regressed females, with a mean binding capacity of 310 +/- 18 nm. sbp levels increased slightly with the appearance of cortica ... | 1997 | 9096875 |
| evaluation of bioassays to monitor surface microlayer toxicity in tropical marine waters. | bioassays were developed, using embryos of: coral,montastraea faveolata; graysby, epinephelus cruentatus;grouper, epinephelus adscensionis x gruttatus (hybrid); queenconch, strombus gigas; rock-boring urchin, echinodermatalucunter; spotted seatrout, cynoscion nebulosus; variegatedurchin, lytechinus variegatus; winged pearl oyster, pteriacolymbus; and yellowtail snapper, ocyurus chrysurus. relativesensitivities and precison of various species-endpoint combinations wereevaluated using three refere ... | 1997 | 9069187 |
| the ovarian progestogen receptor in the spotted seatrout, cynoscion nebulosus, demonstrates steroid specificity different from progesterone receptors in other vertebrates. | a nuclear progestogen receptor has previously been characterized in the ovary of the spotted seatrout. the steroid specificity of this receptor was further defined in the present study by determining the binding affinity of a wide variety of progestin and corticosteroid agonists and antagonists. the addition of a hydroxyl or keto group to the 11 position resulted in a 10-100-fold decrease in relative binding affinity (rba). the significance of the 17, 20, and 21 positions in determining the rba ... | 1997 | 9182865 |
| characterization of a progestogen receptor in the ovary of the spotted seatrout, cynoscion nebulosus. | a nuclear progestogen receptor was identified in the ovary of the spotted seatrout, cynoscion nebulosus. a single class of high-affinity, low-capacity cytoplasmic binding sites for 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-p) was characterized by saturation and scatchard analyses (kd = 1.89 +/- 0.61 nm, bmax = 1.80 +/- 0.63 pmol/g ovary, n = 4), as well as by one-point assay (bmax = 1.41 +/- 0.26 pmol/g ovary, n = 12). analysis of the binding kinetics indicated a fairly rapid ... | 1995 | 7756460 |
| partial characterization of a sex-steroid binding protein in the spotted seatrout (cynoscion nebulosus). | a sex-steroid binding protein (sbp) that binds both testosterone and estradiol-17 beta was identified in the plasma and ovarian interstitial fluids of the spotted seatrout, cynoscion nebulosus. scatchard analysis revealed a single binding component with dissociation constants of 4.89 +/- 0.19 and 3.13 +/- 0.11 nm and binding capacities of 423 +/- 25 and 482 +/- 24 nm for testosterone and 17 beta-estradiol respectively (n = 5). competition studies demonstrated that the seatrout sbp had a high aff ... | 1994 | 7849199 |
| comparative visual function in five sciaenid fishes inhabiting chesapeake bay. | maintaining optimal visual performance is a difficult task in the photodynamic coastal and estuarine waters in which western north atlantic sciaenid fishes support substantial commercial and recreational fisheries. unavoidable tradeoffs exist between visual sensitivity and resolution, yet sciaenid visual systems have not been characterized despite strong species-specific ecomorphological and microhabitat differentiation. we therefore used electroretinographic techniques to describe the light sen ... | 2008 | 18978225 |
| permanent genetic resources added to molecular ecology resources database 1 december 2011-31 january 2012. | this article documents the addition of 473 microsatellite marker loci and 71 pairs of single-nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: barteria fistulosa, bombus morio, galaxias platei, hematodinium perezi, macrocentrus cingulum brischke (a.k.a. m. abdominalis fab., m. grandii goidanich or m. gifuensis ashmead), micropogonias furnieri, nerita melanotragus, nilaparvata lugens stål, sciaenops ocellat ... | 2012 | 22448966 |
| isolation of maturational gonadotropin subunits from spotted seatrout (cynoscion nebulosus) and development of a beta-subunit-directed radioimmunoassay for gonadotropin measurement in sciaenid fishes. | maturational gonadotropin (gth) subunits were isolated from pituitaries of the spotted seatrout (cynoscion nebulosus), a marine perciform teleost, by ethanolic extraction and ion-exchange, gel-filtration, and reverse-phase chromatography. partial amino acid sequencing of the n-terminal regions of the alpha and beta subunits indicated 60-80% identities with various carp and salmon gth subunits. the spotted seatrout gth beta-subunit was used as radioligand in a radioimmunoassay (ria) with atlantic ... | 2006 | 1426954 |
| evidence for a role of na+,k(+)-atpase in the hydration of atlantic croaker and spotted seatrout oocytes during final maturation. | during final maturation the oocytes of many marine teleosts swell four to five times their original size due to uptake of water. the involvement of active inorganic ion transport and na+,k(+)-atpase in oocyte hydration in atlantic croaker (micropogonias undulatus) and spotted seatrout (cynoscion nebulosus), marine teleosts which spawn pelagic eggs, was investigated by examining changes in the inorganic ion content of ovarian follicles containing mainly oocytes, by performing in vitro incubations ... | 1991 | 1651366 |
| changes in hepatic estrogen-receptor concentrations during the annual reproductive and ovarian cycles of a marine teleost, the spotted seatrout, cynoscion nebulosus. | the concentrations of estrogen and vitellogenin in plasma, and hepatic estrogen receptor in cytosolic (erc) and nuclear (ern) extracts were elevated throughout the reproductive season in females from a wild population of spotted seatrout, cynoscion nebulosus. there were significant correlations between plasma estradiol and erc and between ern and plasma vitellogenin during the period of ovarian recrudescence (january-may), but not during the remainder of the reproductive season (june-september). ... | 1991 | 2019398 |
| characterization of membrane receptor activity for 17 alpha, 20 beta, 21-trihydroxy-4-pregnen-3-one in ovaries of spotted seatrout (cynoscion nebulosus). | the proposed maturation-inducing substance (mis) of spotted seatrout (cynoscion nebulosus) is 17 alpha, 20 beta, 21-trihydroxy-4-pregnen-3-one (20 beta-s). in this study, we characterized the binding of radioactive 20 beta-s to plasma membranes from the ovaries of spotted seatrout. bound 20 beta-s was isolated by filtration of membrane suspensions and quantified by measurement of the radioactivity content of the filters. the saturable component of 20 beta-s binding reached equilibrium within 5 m ... | 1990 | 2354763 |
| binding characteristics of the hepatic estrogen receptor of the spotted seatrout, cynoscion nebulosus. | estrogen receptors were identified in cytosolic and nuclear extracts of livers of adult female spotted seatrout, cynoscion nebulosus. a single class of high affinity binding sites was found, kd = 1.26 +/- 0.55 nm (n = 55) for the cytosolic extract and kd = 1.96 +/- 0.42 nm (n = 8) for the nuclear extract. the kd did not differ between males and females or between vitellogenic and nonvitellogenic females. the binding in both the cytosolic and nuclear extracts was specific for estrogens (des great ... | 1990 | 2295421 |
| evidence that 17α,20β,21-trihydroxy-4-pregnen-3-one is a maturation-inducing steroid in spotted seatrout. | ovarian steroidogenesis during final oocyte maturation (fom) in the spotted seatrout (cynoscion nebulosus) was investigated by incubating ovarian fragments with tritiated pregnenolone, followed by chromatographic separation of the radioactive products. the major tritiated steroid produced during fom comigrated with 17α,20β,21-trihydroxy-4-pregnen-3-one (20β-dihydro-11-deoxycortisol, 20β-s) on hplc and tlc. only minor amounts of radioactive material coeluted with 17α,20β-dihydroxy-4-pregnen-3-one ... | 1989 | 24221771 |
| the measurement of plasma vitellogenin levels in a marine teleost, the spotted seatrout (cynoscion nebulosus) by homologous radioimmunoassay. | 1. vitellogenin was purified from plasma of estrogen-treated spotted seatrout (cynoscion nebulosus) and was shown to be a protein of approximately 200,000 mol. wt. 2. antisera were raised against the isolated protein and were used to develop a radioimmunoassay for spotted seatrout vitellogenin. 3. the substance measured was shown to be female-specific, hepatically-derived, estrogen-inducible and immunologically related to oocyte yolk proteins. 4. vitellogenin levels in the plasma of female spott ... | 1988 | 3197389 |
| electrophoretic investigation of subpopulations of the spotted seatrout. cynoscion nebulosus (cuvier), in the gulf of mexico and atlantic coast of florida. | | 1976 | 1269244 |