| purification, properties, and mode of action of hemicellulase i produced by ceratocystis paradoxa. | a culture isolate (cp2) of the fungal plant pathogen ceratocystis paradoxa produces at least five extra-cellular hemicellulases when grown on a medium containing a commercial hemicellulose as inducer. one of the five enzymes, hemicellulase i (hc-i), was purified by ammonium sulphate preceipitation, ion-exchange chromatography (deae-sephadex and then cellex-cm), and iso-electric focusing at ph 3-10 and 8-10. hc-i behaves as a single protein on a electrophoresis at ph 6.0 and 8.4. the enzyme degra ... | 1975 | 234297 |
| cellulase enzymes in culture filtrates of ceratocystis paradoxa. | | 1976 | 995139 |
| structures of the oligosaccharides from the enzymic hydrolysis of hemicellulose by a hemicellulase of ceratocystis paradoxa. | and endo-hemicellulase (hc-ii) of ceratocystis paradoxa degraded spear-grass hemicellulose b to a series of mixed oligosaccharides. four neutral oligosaccharides (araxyl2, araxyl3, xyl2, and xyl3), isolated by preparative paper chromatography, were shown by enzymic and methylation techniques to constitute a series of beta-(1 leats to 4)-d-xylose and o-alpha-l-arabinofuranosyl-(1 leads to 3)-o-beta-d-xylopyranosyl-(1 leads to 4)-o-beta-d-xylopyranosyl-(1 leads to 4)-d-xylose, respectively, the la ... | 1975 | 1192438 |
| heterogeneity of the glucoamylase components of the raw-starch-digesting amylase from chalara paradoxa. | | 1992 | 1499035 |
| effect of plant extracts and systemic fungicide on the pineapple fruit-rotting fungus, ceratocystis paradoxa. | antifungal activities of extracts of sixteen plants were tested against ceratocystis paradoxa which causes soft rot of pineapples. xanthium strumarium was the most effective followed by allium sativum. the effectiveness of various extracts against c. paradoxa was in the decreasing order of meriandra bengalensis, mentha piperita, curcuma longa, phlogacanthus thyrsiflorus, toona ciliata, vitex negundo, azadirachta indica, eupatorium birmanicum, ocimum sanctum and leucas aspera. extracts of cassia ... | 1996 | 9022263 |
| synthesis, characterisation and antifungal activity of some n-bridged heterocycles derived from 3-(3-bromo-4-methoxyphenyl)-4-amino-5-mercapto-1,2,4-triazole. | 3-(3-bromo-4-methoxyphenyl)-4-amino-5-mercapto-1,2,4-triazole was prepared starting from p-anisic acid. this new triazole was employed in the synthesis of some n-bridged heterocycles. all the newly synthesized compounds were characterized by analytical, ir, 1h-nmr and mass spectral studies. some of the newly synthesized compounds were screened for their antifungal property against thielaviopsis paradoxa, a fungus which is reported to cause stem-bleeding disease in coconut plants. | 1996 | 9050212 |
| identification and characterization of a novel arabinoxylanase from wheat flour. | an endogenous wheat (triticum aestivum) flour endoxylanase was purified to homogeneity from a crude wheat flour extract by ammonium sulfate precipitation and cation-exchange chromatography. the 30-kd protein had an isoelectric point of 9.3 or higher. a sequence of 19 amino acids at the nh2 terminus showed 84.2% identity with an internal sequence of 15-kd grain-softness protein, friabilin. high-performance anion-exchange chromatography and gel-permeation analysis of the hydrolysis products indica ... | 1997 | 9414565 |
| steroid transformations with exophiala jeanselmei var. lecanii-corni and ceratocystis paradoxa. | the fungi exophiala jeanselmei var. lecanii-corni [imi (international mycological institute) 312989, uamh (university of alberta microfungus collection and herbarium) 8783] and ceratocystis paradoxa (imi 374529, uamh 8784) have been examined for their potential in steroid biotransformation. the study has determined that e. jeanselmei var. lecanii-corni effected overall anti-markovnikov hydration on dehydroisoandrosterone, and side-chain degradation on a variety of pregnanes. both ascomycetes wer ... | 1999 | 10577834 |
| phenol-oxidase (laccase) activity in strains of the hyphomycete chalara paradoxa isolated from olive mill wastewater disposal ponds. | production of laccase activity by nine strains of chalara paradoxa isolated from olive mill wastewater disposal ponds were studied. enzyme extracts obtained from cultured broths by adsorption on hydroxyapatite showed a single band of laccase activity on abts after polyacrylamide gel electrophoresis (page). they showed small mobility differences, with molecular masses of 67 to 68 kda. enzymes from the different strains oxidized a variety of phenolic and non-phenolic substances, and they could be ... | 2000 | 10771050 |
| beta-glucosidase from chalara paradoxa ch32: purification and properties. | the hyphomycete chalara paradoxa ch32 produced an extracellular beta-glucosidase during the trophophase. the enzyme was purified to homogeneity by ion-exchange and size-exclusion chromatography. the purified enzyme had an estimated molecular mass of 170 kda by size-exclusion chromatography and 167 kda by sds-page. the enzyme had maximum activity at ph 4.0-5.0 and 45 degrees c. the enzyme was inactivated at 60 degrees c. at room temperature, it was unstable at acidic ph, but it was stable to alka ... | 2000 | 10956173 |
| production, purification, and properties of an endoglucanase produced by the hyphomycete chalara (syn. thielaviopsis) paradoxa ch32. | the hyphomycete chalara (syn. thielaviopsis) paradoxa produces endoglucanase activity during the late trophophase. the low molecular mass (35 kda) endoglucanase purified from cultured broths works optimally at 37 degrees c and ph 5.0. the enzyme inactivates at ph below 3.0 and also at temperatures of 50 degrees c or higher, but it is stable at lower temperatures, including refrigeration temperature and freezing. the enzyme is inhibited by detergents, by edta, and by the divalent cations hg(2+) a ... | 2001 | 11170563 |
| growth factor requirements of six fungi associated with fruit decay. | esposito, r. g. (united fruit co., norwood, mass.), h. greenwood, and a. m. fletcher. growth factor requirements of six fungi associated with fruit decay. j. bacteriol. 83:-250-255. 1962.-a study of the growth factor requirements of representatives of six genera of fruit-rotting fungi has been made. gloeosporium musarum, nigrospora sphaerica, thielaviopsis paradoxa, and verticillium theobromae all required biotin for growth. under certain conditions in nitrate medium, fusarium roseum appeared to ... | 1962 | 16561927 |
| in vitro antagonism of thielaviopsis paradoxa by trichoderma longibrachiatum. | seventy-nine trichoderma strains were isolated from soil taken from 28 commercial plantations of agave tequilana cv. 'azul' in the state of jalisco, mexico. nine of these isolates produced nonvolatile metabolites that completely inhibited the growth of thielaviopsis paradoxa on potato dextrose agar plates. these isolates were identified as trichoderma longibrachiatum on the basis of their morphology and dna sequence analysis of two genes (its rdna and translation elongation factor ef-1alpha). my ... | 2007 | 17216331 |
| inhibitory activity against plant pathogenic fungi of extracts from myoporum bontioides a. gray and indentification of active ingredients. | in order to understand the bioactivity of myoporum bontioides a. gray against plant pathogens and determine its active ingredients, the inhibitory activities of methanol extracts from m. bontioides against fusarium oxysporum f. sp. niveum (e. f. smith) snyder & hansen, pestalotia mangiferae p. henn., thielaviopsis paradoxa (de seynes) v. hohnel, colletotrichum musae (berk. & m. a. curtis) v. arx, alternaria alternata (fr.) keissler, mycosphaerella sentina (fr.) schroter and sphaceloma fawcettii ... | 2008 | 18069657 |
| alcohol fermentation of corn starch digested by chalara paradoxa amylase without cooking. | alcohol fermentation of corn starch without cooking was performed by using chalara paradoxa glucoamylase preparation, which had stronger raw starch digesting activity than those of the conventionally known glucoamylases. a raw corn starch-enzyme-yeast mixture was fermented optimally at ph 5.0 and 30 degrees c for five days and produced ethanol. the yields of ethanol were between 63.5 and 86.8% of the theoretical value by baker's yeast (saccharomyces cerevisiae), and between 81.1 and 92.1% of the ... | 1987 | 18576507 |
| repeating use of chalara paradoxa amylase by alpha-cyclodextrin affinity column. | | 1988 | 18584767 |
| physico-chemical and antifungal properties of protease inhibitors from acacia plumosa. | this study was aimed at investigating the purification, biological activity, and some structural properties of three serine protease inhibitors isoforms, denoted aptia, aptib, and aptic from acacia plumosa lowe seeds. they were purified from the saline extract of the seeds, using superdex-75 gel filtration and mono-s ion exchange chromatography. they were further investigated by mass spectrometry, spectroscopic measurements, surface plasmon resonance, and inhibition assays with proteases and phy ... | 2009 | 19443001 |
| production of cellulolytic enzymes by fungi acrophialophora nainiana and ceratocystis paradoxa using different carbon sources. | although a number of filamentous fungi, such as trichoderma and aspergillus, are well known as producers of cellulases, xylanases, and accessory cellulolytic enzymes, the search for new strains and new enzymes has become a priority with the increase in diversity of biomass sources. moreover, according to the type of pretreatment applied, biomass of the same type may require different enzyme blends to be efficiently hydrolyzed. this study evaluated cellulases, xylanases, and beta-glucosidases pro ... | 2010 | 20174889 |
| hydroxylation of steroids by fusarium oxysporum, exophiala jeanselmei and ceratocystis paradoxa. | the potential of fusarium oxysporum var. cubense uamh 9013 to perform steroid biotransformations was reinvestigated using single phase and pulse feed conditions. the following natural steroids served as substrates: dehydroepiandrosterone (1), pregnenolone (2), testosterone (3), progesterone (4), cortisone (5), prednisone (6), estrone (7) and sarsasapogenin (8). the results showed the possible presence of c-7 and c-15 hydroxylase enzymes. this hypothesis was explored using three synthetic androst ... | 2011 | 21763336 |