| using protein synthesis inhibitors to establish the phylogenetic relationships of the sulfolobales order. | the sensitivity of the cell-free protein synthesis systems from acidanus brierleyi, acidianus infernus, and metallosphaera sedula, members of the archaeal order sulfolobales, to 40 antibiotics with different specificities has been studied. the sensitivity patterns were compared to those of sulfolobus solfataricus and other archaeal, bacterial, and eukaryotic systems. the comparative analysis shows that ribosomes from the sulfolobales are the most refractory to inhibitors of protein synthesis des ... | 1994 | 7807541 |
| presence of acetyl coenzyme a (coa) carboxylase and propionyl-coa carboxylase in autotrophic crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation. | the pathway of autotrophic co2 fixation was studied in the phototrophic bacterium chloroflexus aurantiacus and in the aerobic thermoacidophilic archaeon metallosphaera sedula. in both organisms, none of the key enzymes of the reductive pentose phosphate cycle, the reductive citric acid cycle, and the reductive acetyl coenzyme a (acetyl-coa) pathway were detectable. however, cells contained the biotin-dependent acetyl-coa carboxylase and propionyl-coa carboxylase as well as phosphoenolpyruvate ca ... | 1999 | 9973333 |
| the unusual iron sulfur composition of the acidianus ambivalens succinate dehydrogenase complex. | the succinate dehydrogenase complex of the thermoacidophilic archaeon acidianus ambivalens was investigated kinetically and by epr spectroscopy in its most intact form, i.e., membrane bound. here it is shown that this respiratory complex has an unusual iron-sulfur cluster composition in respect to that of the canonical succinate dehydrogenases known. the spectroscopic studies show that center s3, the succinate responsive [3fe-4s]1+/0 cluster of succinate dehydrogenases, is not present in membran ... | 1999 | 10216159 |
| acidophiles of saline water at thermal vents of vulcano, italy. | dna was extracted from samples taken from close to acidic hydrothermal vents on shore of the aeolian island of vulcano (italy). rna gene sequences were amplified by pcr, cloned, and sequenced. a sequence with an origin in samples at 35 degrees and 45 degrees c corresponded to that of a novel acidithiobacillus species that was isolated from water close to the vents. novel, iron-oxidizing mesophilic acidophiles were isolated through enrichment cultures with ferrous iron but were not represented in ... | 2002 | 12072955 |
| diversity of thermophilic and non-thermophilic crenarchaeota at 80 degrees c. | a hot spring in the solfataric field of pisciarelli (naples-italy) was analysed for archaeal diversity. total dna was extracted from the environment, archaeal 16s rrna genes were amplified with archaea specific primers, and a clone library consisting of 201 clones was established. the clones were grouped in 10 different groups each representing a specific band pattern using restriction fragment length polymorphism (rflp). members of all 10 groups were sequenced and phylogenetically analyzed. sur ... | 2005 | 15727822 |
| novel thermostable y-family polymerases: applications for the pcr amplification of damaged or ancient dnas. | for many years, taq polymerase has served as the stalwart enzyme in the pcr amplification of dna. however, a major limitation of taq is its inability to amplify damaged dna, thereby restricting its usefulness in forensic applications. in contrast, y-family dna polymerases, such as dpo4 from sulfolobus solfataricus, can traverse a wide variety of dna lesions. here, we report the identification and characterization of five novel thermostable dpo4-like enzymes from acidianus infernus, sulfolobus sh ... | 2006 | 16488882 |
| archaeal diversity in two thermophilic chalcopyrite bioleaching reactors. | this study used a culture-independent molecular approach to investigate the archaeal community composition of thermophilic bioleaching reactors. two culture samples, mtc-a and mtc-b, grown with different concentrations of chalcopyrite (cufes2), a copper sulfidic ore, at a temperature of 78 degrees c and ph 1.6 were studied. phylogenetic analysis of the 16s rrna genes revealed that both cultures consisted of archaea belonging to the sulfolobales. the 16s rrna gene clone library of mtc-a grown wit ... | 2006 | 17014503 |
| acidianus manzaensis sp. nov., a novel thermoacidophilic archaeon growing autotrophically by the oxidation of h2 with the reduction of fe3+. | a novel thermoacidophilic iron-reducing archaeon, strain na-1, was isolated from a hot fumarole in manza, japan. strain na-1 could grow autotrophically using h2 or s0 as an electron donor and fe3+ as an electron acceptor, and also could grow heterotrophically using some organic compounds. fe3+ and o2 served as electron acceptors for growth. however, s0, no3-, no2-, so4(2-), mn4+, fumarate, and fe2o3 did not serve as electron acceptors. the ranges of growth temperature and ph were 60-90 degrees c ... | 2006 | 17066338 |
| acidianus sulfidivorans sp. nov., an extremely acidophilic, thermophilic archaeon isolated from a solfatara on lihir island, papua new guinea, and emendation of the genus description. | a novel, extremely thermoacidophilic, obligately chemolithotrophic archaeon (strain jp7(t)) was isolated from a solfatara on lihir island, papua new guinea. cells of this organism were non-motile, gram-negative staining, irregular-shaped cocci, 0.5-1.5 microm in size, that grew aerobically by oxidation of sulfur, fe(2+) or mineral sulfides. cells grew anaerobically using fe(3+) as a terminal electron acceptor and h(2)s as an electron donor but did not oxidize hydrogen with elemental sulfur as el ... | 2007 | 17625168 |
| probing the archaeal diversity of a mixed thermophilic bioleaching culture by tgge and fish. | the archaeal community present in a sample of mixed thermophilic culture-b (mtc-b) from a laboratory-scale thermophilic bioleaching reactor was investigated by temperature gradient gel electrophoresis (tgge) and fluorescence in situ hybridisation (fish). both techniques were specifically adapted for use on native state bioleaching samples, with a view to establishing convenient means for monitoring culture composition. using the tgge protocol developed, the relative species composition of the th ... | 2009 | 19541445 |
| physiologic versatility and growth flexibility as the main characteristics of a novel thermoacidophilic acidianus strain isolated from copahue geothermal area in argentina. | a novel thermoacidophilic archaeal strain has been isolated from three geothermal acidic hot springs in copahue, argentina. one of the most striking characteristic of ale1 isolate is its metabolic versatility. it grows on sulphur, tetrathionate, iron (ii) and sucrose under aerobic conditions, but it can also develop under anaerobic conditions using iron (iii) or sulphur as electron acceptors and sulphur or hydrogen as electron donors autotrophically. a temperature of 75 °c and a ph between 2.5 a ... | 2013 | 23052926 |