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construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees.infections with different viroid species are common among cultivated fruit trees and grapevines, and many old-clone citrus varieties contain up to five citrus viroids (cvds) within a single tree. this paper describes the construction of a cvd-multiprobe consisting of full-length clones of hop stunt viroid, citrus exocortis viroid, citrus bent leaf viroid and cvd-iii. the cvd-multiprobe was tested against rna transcripts of the four viroids and rna extracts from plants singly infected with cevd o ...200616990999
nucleotide sequence of a new viroid species, citrus bent leaf viroid (cblvd) isolated from grapefruit in israel. 20071891367
phloem restriction of viroids in three citrus hosts is overcome by grafting with etrog citron: potential involvement of a translocatable factor.viroid systemic spread involves cell-to-cell movement from initially infected cells via plasmodesmata, long-distance movement within the phloem and again cell-to-cell movement to invade distal tissues including the mesophyll. citrus exocortis viroid (cevd), hop stunt viroid, citrus bent leaf viroid, citrus dwarfing viroid, citrus bark cracking viroid and citrus viroid v remained phloem restricted when singly infecting citrus karna, citrus aurantium and poncirus trifoliata, but not etrog citron, ...201525888624
variability of the progeny of a sequence variant citrus bent leaf viroid (cblvd).a field isolate of cblvd was previously shown to contain two dominant subpopulations (i and ii), which differed by the presence or absence of a sal i restriction site in the pcr product [10]. here we demonstrate the infectivity and symptom expression of subpopulation ii by inoculating etrog citron with a single representative haplotype. the resulting progeny was characterised as an heterogeneous population of closely related variants with a new fitness peak represented by an haplotype that was n ...200414745604
a population of variants of a viroid closely related to citrus viroid-i in citrus plants.sequencing analyses showed a population of variants consisting of 325-330 nucleotides (nt) of a viroid closely related to citrus viroid (cvd)-i in citrus plants. these variants, for which we propose the tentative acronym cvd-i-lss (low sequence similarity), have only 82-85% sequence similarities to cvd-i variants. a phylogenetic tree showed that the cvd-i-lss variants formed an individual cluster that was distinct from that of the cvd-i variants, but no intermediate variants were found which cou ...200011087094
citrus viroid ia is a derivative of citrus bent leaf viroid (cvd-ib) by partial sequence duplications in the right terminal region.nucleotide sequences of group i citrus viroids ia (cvd-ia) and citrus bent leaf viroid (cblvd, formerly designated cvd-ib) isolated from citrus plants in japan, the philippines and china have been determined. citrus samples in japan and the philippines contained cvd-ia, which consists of 328 nucleotides(nt). although 10 nt longer than the type cblvd-225a in israel they share 94% identity in overall nucleotide sequence. the philippines sample also contained a 329-nt long cvd-ia sequence variant, ...19989645202
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