| occurrence of nepoviruses in rubus species in the czech republic. | the occurrence of arabis mosaic virus (amv), raspberry ringspot virus (rrv), tomato black ring virus (tbrv), strawberry latent ringspot virus (slrv) and cherry leaf roll virus (clrv) in cultivated and wild plants of raspberry and blackberry has been studied in the czech republic in 1993-1996. five hundred and seventy samples were collected at 51 localities and assayed by double antibody sandwich enzyme-linked immunosorbent assay (das-elisa). the results represent the first evidence on the occurr ... | 1997 | 9385408 |
| differences between the coat protein amino acid sequences of english and scottish serotypes of raspberry ringspot virus exposed on the surface of virus particles. | the region of the rna 2 coding for the putative helper/movement protein and the coat protein (cp) of each of six isolates of raspberry ringspot virus was sequenced and these sequences were compared with the published sequence of the scottish type isolate. minimal differences were detected among the putative translations of the helper/movement proteins, however, multiple alignment and phylogenetic analysis of the putative cps separated the english and scottish serotypes into two distinct clades. ... | 2000 | 10958983 |
| transmission of raspberry ringspot virus by longidorus elongatus (de man) (nematoda: dorylaimidae). | | 1962 | 13919927 |
| cross-reacting and heterospecific monoclonal antibodies produced against arabis mosaic nepovirus. | monoclonal antibodies (mabs) were produced against arabis mosaic nepovirus (amv). a hybridoma screening procedure was applied which involved the testing of culture supernatants, before the hybridomas were cloned to single cell lines, for their reaction with eight nepoviruses [amv, cherry leafroll virus (clrv), grapevine fanleaf virus (gflv), peach rosette mosaic virus, raspberry ringspot virus (rrsv), tobacco ringspot virus, tomato black ring virus (tbrv) and tomato ringspot virus]. in addition ... | 1992 | 1402798 |
| complete nucleotide sequence of an isolate of the nepovirus raspberry ringspot virus from grapevine. | the complete nucleotide sequence of the rnas 1 and 2 of the nepovirus raspberry ringspot virus cherry isolate (rprsv-ch) from grapevine was determined. the rna 1 is 7935 nucleotides (nt) long excluding the poly(a) tail, and contains one long open reading frame (orf) encoding a polypeptide of 2367 amino acids. this orf is preceeded by a 136nt 5' non-coding region, and followed by a 695nt 3' non-coding region. conserved amino acid motifs, characteristic of the viral protease cofactor, the ntp-bind ... | 2003 | 14602206 |
| sequence analysis of grapevine isolates of raspberry ringspot nepovirus. | the nucleotide sequences of rnas 1 and 2 of a german isolate of raspberry ringspot virus (rprsv) infecting grapevine (rprsv-grapevine), as well as partial sequences of another grapevine isolate from switzerland (rac815) were determined. the sequences of the protease-polymerase region encoded by rna1, and the movement protein and coat protein genes encoded by rna 2, of these isolates were compared with those of other isolates available in databases. the coat proteins of the grapevine isolates for ... | 2006 | 16328136 |
| ingestion, retention, and transmission of two strains of raspberry ring-spot virus by longidorus macrosoma. | the transmission of two strains of raspberry ringspot virus (rrv) by small numbers of nematodes was compared. a strain of rrv from scotland (rrv-s), originally found in the field associated with longidorus elongatus, was transmitted frequently by l. elongatus but only once by l. macrosoma. a strain from england (rrv-e) associated with l. macrosoma in the field was transmitted infrequently by each species of nematode. the reasons why l. macrosoma infected only a small proportion of bait plants wi ... | 1978 | 19305817 |
| novel approaches to mitigate primer interaction and eliminate inhibitors in multiplex pcr, demonstrated using an assay for detection of three strawberry viruses. | multiplex pcr is an important technique for detecting a variety of pathogens simultaneously in a single assay. previous research has focused on optimising the factors affecting reliable multiplex pcr, including primer design, pcr components and conditions, and inhibitors in samples. in this study, the interaction of primers to form complex secondary structures including visible dimers and invisible "primer clusters", a novel form of primer secondary structure found during this research, were sho ... | 2008 | 18453003 |
| cloning and sequencing of the coat protein gene of tobacco ringspot virus isolates from uk and iran. | the coat protein (cp) gene and the 3' untranslated region (utr) of genomic rna 2 of tobacco ringspot virus (trsv, the genus nepovirus, subgroup a) isolates from the uk and iran were cloned from total viral rna and sequenced. comparison of these isolates with an isolate from the usa revealed a high degree of nucleotide and amino acid identity which extends the knowledge of molecular relationships between these three trsv isolates. the uk isolate shared the highest nucleotide identity (95%) with t ... | 2001 | 12083332 |