| pear blister canker viroid is a member of the apple scar skin subgroup (apscaviroids) and also has sequence homology with viroids from other subgroups. | the sequence of pear blister canker viroid (pbcvd), the putative causal agent of pear blister canker (pbc) disease, has been determined. pbcvd consists of a single-stranded circular rna of 315 nucleotide residues which assumes a branched conformation when it is folded in the model of lowest free energy. pbcvd has highest sequence similarity with grapevine 1b viroid (52.4%), but also contains sequences related to regions present in viroids that belong to different subgroups, suggesting that pbcvd ... | 1992 | 1383394 |
| pear blister canker viroid: sequence variability and causal role in pear blister canker disease. | the sequences of several cdna clones of pear blister canker viroid (pbcvd) p1914t and p47a isolates have been determined. seven out of eight p1914t clones analysed have a constant sequence which differs at six positions from that of the p2098t isolate reported previously. the remaining p1914t clone (8) has a single nucleotide substitution. the same six changes have been also observed in most of the ten p47a clones sequenced. however, some p47a clones show additional variability in positions on b ... | 1995 | 7595369 |
| a novel multiplex rt-pcr probe capture hybridization (rt-pcr-elisa) for simultaneous detection of six viroids in four genera: apscaviroid, hostuviroid, pelamoviroid, and pospiviroid. | a rapid and sensitive assay was developed for the detection and identification of viroids by standard or multiplex reverse transcription-polymerase chain reaction (rt-pcr)-probe capture hybridization (rt-pcr-elisa). the assay was applied successfully for the detection and identification of the following six viroid species from infected tissues: potato spindle tuber viroid (pospiviroid), peach latent mosaic viroid (pelamoviroid), apple scar skin viroid (apscaviroid), apple dimple fruit viroid (ap ... | 2002 | 12176148 |
| development of a one tube-one step rt-pcr protocol for the detection of seven viroids in four genera: apscaviroid, hostuviroid, pelamoviroid and pospiviroid. | a one tube-one step rt-pcr was developed for the detection of seven viroids (apple scar skin viroid, apple dimple fruit viroid, pear blister canker viroid, hop stunt viroid, chrysanthemum stunt viroid, citrus exocortis viroid and peach latent mosaic viroid) in four genera that infect eight plant species. the efficiency and specificity of this method were optimized by the use of moloney-murine leukemia virus (m-mlv) reverse-transcriptase and hotstartaq dna polymerase which allowed increase sensit ... | 2004 | 15350729 |
| one-step multiplex quantitative rt-pcr for the simultaneous detection of viroids and phytoplasmas of pome fruit trees. | a one-step multiplex real-time quantitative reverse transcription polymerase chain reaction (rt-qpcr) based on taqman chemistry was developed for the simultaneous detection of pear blister canker viroid and apple scar skin viroid along with universal detection of phytoplasmas, in pome trees. total nucleic acids (tnas) extraction was performed according to a modified ctab protocol. primers and taqman mgb probes for specific detection of the two viroids were designed in this study, whereas for phy ... | 2015 | 25479356 |