| processing of structural polypeptides of infectious flacherie virus of the silkworm, bombyx mori: vp1 and vp4 are derived from vp0. | infectious flacherie virus of the silkworm, bombyx mori, contains five major polypeptides termed vp0, vp1, vp2, vp3, and vp4 in the order of descending molecular weight. immunoblot analysis with specific antisera against each of these structural polypeptides showed that antisera against vp1 and vp4 unequivocally reacted with vp0 as well as their homologous structural polypeptides. limited proteolysis of vp0 and vp1 by staphylococcus aureus v8 protease gave several common polypeptide fragments. a ... | 1992 | 1401988 |
| virucidal activity and viral protein modification by myeloperoxidase: a candidate for defense factor of human polymorphonuclear leukocytes against influenza virus infection. | influenza virus (ifv) was inactivated by treatment with degranulated fluid (df) or myeloperoxidase (mpo) from human polymorphonuclear leukocytes in the presence of h2o2. hemagglutinin and neuraminidase activities of the virus were also reduced by df treatment. in addition, treatment with df or mpo in the presence of h2o2 resulted in delayed migration of viral proteins in sds-page, indicating extensively modified viral proteins. parallel with this aberrant migration, the radioactivity of each pro ... | 1991 | 1647427 |
| antiviral activities and the mechanism of 9-beta-d-ribofuranosyl-6-alkylthiopurines on several rna viruses from animals. | a series of 9-beta-d-ribofuranosyl-6-alkylthiopurines (6-alkyl ti) were found to inhibit in vitro replication of infectious hematopoietic necrosis virus (ihnv), human influenza virus (ifv) and respiratory syncytial virus (rsv) with ic50 values of about 0.06 microgram/ml, 0.7-1.5 micrograms/ml and 1-3 micrograms/ml, respectively. viral rna synthesis in infected cells in the presence of actinomycin d was inhibited by treatment with the compounds dose-dependently. it was also found that the decreas ... | 1989 | 2608474 |
| changes in infectious flacherie virus-specific polypeptides and translatable mrna in the midgut of the silkworm, bombyx mori, during larval molt. | | 1989 | 2915146 |
| phagocytosis and ingestion of influenza virus by human polymorphonuclear leucocytes in vitro: electronmicroscopy studies. | interaction of human polymorphonuclear leucocytes (pmnl) and influenza virus (ifv) was studied in vitro. at 0 degree c, the viral particles were bound extensively to the surface of the pmnl membrane with a ratio of about 1000 virus particles to a single pmnl. the binding was sensitive to neuraminidase, suggesting attachment through sialo-compound receptors. at 37 degrees c, the virus particles disappeared rapidly from the cell surface, about half of them being eluted and the remainder being endo ... | 1989 | 2926790 |
| evidence for the presence of a genome-linked protein in infectious flacherie virus. | the genomic rna of infectious flacherie virus (ifv), an insect picornavirus, was shown to contain a covalently attached substance which can be labeled with 125i-bolton and hunter reagent. this substance was found to be a protein covalently linked to the virus genome, because it cosedimented and was copurified with the viral rna and was degraded by proteinase k but not by rnases. the molecular weight of the protein was found to be 11,500 in the sodium dodecyl sulfate polyacrylamide gel electropho ... | 1986 | 3729730 |
| in vitro translation of infectious flacherie virus rna in a wheat germ and a rabbit reticulocyte system. | infectious flacherie virus is an insect picornavirus isolated from the silkworm, bombyx mori. its rna was found to act as an efficient mrna in a wheat germ extract and a rabbit reticulocyte lysate translation system. in either system the sum of molecular weights of translation products far exceeded the coding capacity of the virus genome, which suggests the occurrence of proteolytic cleavage of large primary products to smaller polypeptides as reported for other picornaviruses and/or premature t ... | 1984 | 6696917 |
| analysis of genetic information of an insect picorna-like virus, infectious flacherie virus of silkworm: evidence for evolutionary relationships among insect, mammalian and plant picorna(-like) viruses. | we synthesized the cdnas of an insect picornavirus, infectious flacherie virus of silkworm (ifv), genomic rna and inserted it into a bacterial plasmid (puc119). the 9,650 nucleotides (nts) sequence except for the poly(a) tail was obtained from the cloned cdnas, and the sequence integrity was confirmed by primer extension and direct rna sequencing. the sequence has a large open reading frame (orf) of 9,255 nts (3,085 codons) flanked by the short 5' non-coding region (156 nts) and by the rather lo ... | 1998 | 9505971 |
| protein a linked latex antisera test for detection of infectious flacherie of silkworm, bombyx mori l. caused by b. mori infectious flacherie virus. | protein a linked latex antisera (pallas) test was used for detection of b. mori infectious flacherie virus (bmifv) in silkworms. the test was found superior to direct latex agglutination (dla) test for detection of bmifv in infected larval and faecal matter extracts and purified preparations. latex beads sensitized with anti-bmifv igg were able to detect up to 75 ng/ml bmifv in pallas test and 300 ng/ml in dla test. bmifv was detected in diluted extracts of infected larvae (up to 1:200) and thei ... | 1997 | 9567751 |
| the nucleotide sequence of sacbrood virus of the honey bee: an insect picorna-like virus. | we have determined the nucleotide sequence of sacbrood virus (sbv), which causes a fatal infection of honey bee larvae. the genomic rna of sbv is longer than that of typical mammalian picornaviruses (8832 nucleotides) and contains a single, large open reading frame (179-8752) encoding a polyprotein of 2858 amino acids. sequence comparison with other virus polyproteins revealed regions of similarity to characterized helicase, protease and rna-dependent rna polymerase domains; structural genes wer ... | 1999 | 10374974 |
| immunomodulatory function of lactic acid bacteria. | using mice, we found that oral administration of bifidobacterium breve yit4064 (b. breve) activated the humoral immune system, augmented anti-rotavirus iga production or anti-influenza virus (ifv) igg production and protected against rotavirus infection or influenza infection, respectively. furthermore, when the b. breve was given to infants from an infant home, there was a significant reduction of the frequency of rotavirus shedding in stool samples during the administration of the bacteria. it ... | 1999 | 10532394 |
| effect of intranasal administration of lactobacillus casei shirota on influenza virus infection of upper respiratory tract in mice. | in mice administered lactobacillus casei strain shirota (lcs) intranasally, potent induction of interleukin 12, gamma interferon, and tumor necrosis factor alpha, which play a very important role in excluding influenza virus (ifv), was evident in mediastinal lymph node cells. in this model of upper respiratory ifv infection, the titers of virus in the nasal wash of mice inoculated with 200 microg of lcs for three consecutive days (lcs 200 group) before infection were significantly (p < 0.01) low ... | 2001 | 11329464 |
| augmentation of cellular immunity and reduction of influenza virus titer in aged mice fed lactobacillus casei strain shirota. | we investigated whether oral administration of lactobacillus casei strain shirota activates the cellular immune system and ameliorates influenza virus (ifv) titer in the nasal site in upper respiratory ifv infection by using aged mice. natural killer activity of splenocytes and lung cells of aged mice fed an l. casei strain shirota diet (l.casei strain shirota group) was significantly (p < 0.01 and p < 0.05) increased compared to those fed a control diet (control group). the increases were 1.5- ... | 2002 | 11777838 |
| evolutionary and taxonomic implications of conserved structural motifs between picornaviruses and insect picorna-like viruses. | a comparison of the recently determined structure of an insect picorna-like virus, cricket paralysis virus (crpv), with that of the mammalian picornaviruses shows that several structural features are highly conserved between these viruses. these conserved features include the topology of the coat proteins, the conformation of most loops, and the general arrangement of the internally located n-terminal arms of the coat proteins. the conformational conservation of the n-termini of the three major ... | 2002 | 11855636 |
| the complete genome sequence of perina nuda picorna-like virus, an insect-infecting rna virus with a genome organization similar to that of the mammalian picornaviruses. | perina nuda picorna-like virus (pnpv) is an insect-infecting rna virus with morphological and physicochemical characters similar to the picornaviridae. in this article, we determine the complete genome sequence and analyze the gene organization of pnpv. the genome of pnpv consists of 9476 nucleotides (nts) excluding the poly(a) tail and contains a single large open reading frame (orf) of 8958 nts (2986 codons) flanked by 473 and 45 nt noncoding regions on the 5' and 3' ends, respectively. northe ... | 2002 | 12009873 |
| sequence analysis and genomic organization of a new insect picorna-like virus, ectropis obliqua picorna-like virus, isolated from ectropis obliqua. | the complete nucleotide sequence of a new insect picorna-like virus, ectropis obliqua picorna-like virus (eopv), which causes a fatal infection of ectropis obliqua larvae, has been determined. the genomic rna of eopv is 9394 nt in length and contains a single, large open reading frame (nt 391-9351) encoding a polyprotein of 2987 aa. sequence comparisons with other viral polyproteins revealed that the consensus sequences for picornavirus rna helicase, protease and rna-dependent rna polymerase pro ... | 2004 | 15105531 |
| reduction of influenza virus titer and protection against influenza virus infection in infant mice fed lactobacillus casei shirota. | we investigated whether oral administration of lactobacillus casei strain shirota to neonatal and infant mice ameliorates influenza virus (ifv) infection in the upper respiratory tract and protects against influenza infection. in a model of upper respiratory ifv infection, the titer of virus in the nasal washings of infant mice administered l. casei shirota (l. casei shirota group) was significantly (p < 0.05) lower than that in infant mice administered saline (control group) (10(2.48) +/- 10(0. ... | 2004 | 15242940 |
| sialidase fusion protein as a novel broad-spectrum inhibitor of influenza virus infection. | influenza is a highly infectious disease characterized by recurrent annual epidemics and unpredictable major worldwide pandemics. rapid spread of the highly pathogenic avian h5n1 strain and escalating human infections by the virus have set off the alarm for a global pandemic. to provide an urgently needed alternative treatment modality for influenza, we have generated a recombinant fusion protein composed of a sialidase catalytic domain derived from actinomyces viscosus fused with a cell surface ... | 2006 | 16569867 |
| safety of sperm washing and art outcome in 741 hiv-1-serodiscordant couples. | to evaluate the safety of sperm washing and assisted reproduction technique (art) outcome offered to serodiscordant couples with a human immunodeficiency virus-1 (hiv-1)-positive male. | 2007 | 17107974 |
| a feline case of isolated fourth ventricle with syringomyelia suspected to be related with feline infectious peritonitis. | a one-year-old female cat was unable to stand. magnetic resonance imaging was performed, and an enlargement of the lateral, third, and fourth ventricles and syringomyelia were detected. the cat was diagnosed with an isolated fourth ventricle (ifv) with syringomyelia. the serum isoantibody test for the feline infectious peritonitis (fip) virus was 1:3,200. after the cat died, a pathological examination revealed nonsuppurative encephalomyelitis. we suspected that the ifv, detected in the cat, was ... | 2007 | 17675810 |
| [structure and antiviral activity of an acidic polysaccharide from an edible blue-green alga, nostoc flagelliforme]. | recently, the development of antiviral agents with novel mechanisms of action has been required since many types of infectious disease have become a serious problem in our society. in the present study, we isolated a novel acidic polysaccharide, nostoflan (nsf), from a terrestrial blue-green alga, nostoc flagelliforme, and examined its structure and antiviral activity. the sugar composition and methylation analyses of nsf revealed that it is mainly composed of (-->4)- d-glcp-(1-->, -->6,4)-d-glc ... | 2008 | 18451619 |
| [recombinant expression of human influenza a virus nucleocapsid protein and its antigenicity analyses]. | to prepare anti-recombinant protein antibody from immunized mice with recombinant nucleocapsid protein (np) of human influenza a3 (ifv-a3) virus expressed in prokaryotic cell, and to explore the feasibility of utilizing anti-recombinant protein antibody to detect influenza a virus. | 2008 | 19031705 |
| [antiviral effects of an effective section of a prescription of traditional chinese medicine on influenza virus a in vitro]. | to study the inhibitive effects of an effective section of a prescription of traditional chinese medicine (tcm-es) on influenza virus a fm1 strain in vitro. | 2009 | 19565719 |
| novel pandemic influenza a(h1n1) viruses are potently inhibited by das181, a sialidase fusion protein. | the recent emergence of a novel pandemic influenza a(h1n1) strain in humans exemplifies the rapid and unpredictable nature of influenza virus evolution and the need for effective therapeutics and vaccines to control such outbreaks. however, resistance to antivirals can be a formidable problem as evidenced by the currently widespread oseltamivir- and adamantane-resistant seasonal influenza a viruses (ifv). additional antiviral approaches with novel mechanisms of action are needed to combat novel ... | 2009 | 19893747 |
| inhibition of neuraminidase inhibitor-resistant influenza virus by das181, a novel sialidase fusion protein. | antiviral drug resistance for influenza therapies remains a concern due to the high prevalence of h1n1 2009 seasonal influenza isolates which display h274y associated oseltamivir-resistance. furthermore, the emergence of novel h1n1 raises the potential that additional reassortments can occur, resulting in drug resistant virus. thus, additional antiviral approaches are urgently needed. das181 (fludase), a sialidase fusion protein, has been shown to have inhibitory activity against a large number ... | 2009 | 19893749 |
| das181, a sialidase fusion protein, protects human airway epithelium against influenza virus infection: an in vitro pharmacodynamic analysis. | the influenza virus (ifv) infection models commonly used to evaluate antiviral agents (e.g. mdck cell line and mice) are limited by physiological differences from the human respiratory tract in vivo. here we report the pharmacodynamics of das181, a sialidase fusion protein that inhibits influenza infection, in the model systems of well-defined human airway epithelium (hae) culture and ex vivo culture of fresh human bronchial tissue, both of which are close mimics of the human respiratory tract i ... | 2010 | 19942616 |
| the three-dimensional structure of infectious flacherie virus capsid determined by cryo-electron microscopy. | cryo-electron microscopy and image reconstruction were used to determine the three-dimensional structure of infectious flacherie virus (ifv). 5047 particles were selected for the final reconstruction. the fsc curve showed that the resolution of this capsid structure was 18 a. the structure is a psuedo t=3 (p=3) icosahedral capsid with a diameter of 302.4 a and a single shell thickness of 15 a. the density map showed that ifv has a smooth surface without any prominent protrude or depression. comp ... | 2009 | 20016976 |
| sialidase-based anti-influenza virus therapy protects against secondary pneumococcal infection. | das181 (fludase) is a sialidase fusion protein in clinical development as a broad-spectrum anti-influenza virus (ifv) therapeutic agent. previous reports by other investigators have raised the concern that desialylation of airway epithelium might increase susceptibility to streptococcus pneumoniae infection. | 2010 | 20170378 |
| performance of rapid influenza testing in hospitalized children. | influenza infection is associated with high hospitalization rates among young children. rapid diagnosis of influenza infection is particularly useful in order to prevent nosocomial infection and allows for the timely initiation of antiviral treatment. we evaluated the performance of a rapid influenza test in hospitalized children during the influenza season. all children (aged 6 months to 14 years) hospitalized with fever and/or respiratory symptoms, admitted during the 2005 influenza season, pa ... | 2010 | 20349200 |
| characterization of structures and antiviral effects of polysaccharides from portulaca oleracea l. | three polysaccharides including a neutral polysaccharide (rn), an acidic polysaccharide (ra) and a pectic polysaccharide (rp) were isolated from aerial part of portulaca oleracea l. and evaluated for their anti-herpes simplex virus type 2 (hsv-2) and anti-influenza a virus (ifv-a). rn was found to consist of glucose (glc), mannose (man) and arabinose (ara) with small amounts of galactose (gal), and identified to be an arabinoglucomannan. ra was mainly composed of gal and ara with a small proport ... | 2010 | 20410633 |
| intranasal administration of lactobacillus rhamnosus gg protects mice from h1n1 influenza virus infection by regulating respiratory immune responses. | to investigate whether intranasal lactobacillus administration protects host animals from influenza virus (ifv) infection by enhancing respiratory immune responses in a mouse model. | 2010 | 20438620 |
| therapeutic effect of arctiin and arctigenin in immunocompetent and immunocompromised mice infected with influenza a virus. | arctiin and its aglucone, arctigenin from the fruits of arctium lappa l. showed potent in vitro antiviral activities against influenza a virus (a/nws/33, h1n1) (ifv). based on the data from time-of-addition experiments and on release tests of progeny viruses, arctigenin was assumed to interfere with early event(s) of viral replication after viral penetration into cells, and to suppress the release of progeny viruses from the host cells. arctiin was orally effective against either ifv-inoculated ... | 2010 | 20606313 |
| identification of adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus by two kinds of multiplex polymerase chain reaction (pcr) and a shell vial culture in pediatric patients with viral pneumonia. | early identification of causative agents in lower respiratory infection of pediatric patients can reduce morbidity and prevent an overuse of antimicrobials. two kinds of multiplex polymerase chain reaction (pcr) and a commercial shell vial viral culture were performed to identify causative agents in pediatric patients. | 2010 | 20635453 |
| evaluation of an edible blue-green alga, aphanothece sacrum, for its inhibitory effect on replication of herpes simplex virus type 2 and influenza virus type a. | a hot-water extract of aphanothece sacrum, an edible aquacultured blue-green alga, was found to show a remarkable inhibitory effect on the replication of enveloped viruses including herpes simplex virus type 2 (hsv-2) and influenza virus type a (ifv-a, h1n1) in vitro. the main active components were suggested to be sulfated polysaccharides in non-dialyzable portion (aswph). aswph was found to inhibit the viral adsorption to the receptor of the host cells involved in the replication process of hs ... | 2010 | 20699552 |
| potent virucidal effect of pheophorbide a and pyropheophorbide a on enveloped viruses. | in this study, we evaluated the inhibitory effect of ethanol and aqueous extracts from a stem of opuntia ficus indica on replication of three kinds of viruses: two enveloped viruses [herpes simplex virus type 2 (hsv-2), influenza a virus (ifv-a)], and one non-enveloped virus [poliovirus type 1 (pv-1)]. only ethanol extract from the cactus stem showed significant antiviral activity in vitro. two chlorophyll derivatives, pheophorbide a and pyropheophorbide a, were isolated as active substances exh ... | 2011 | 20835849 |
| oral administration of heat-killed lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice. | host-defense mechanisms against influenza virus (ifv) infection involve both innate and acquired immunities. among other components, secretory immunoglobulin a (siga) in the airway mucosa plays a particularly pivotal role in preventing ifv infection. among 150 strains of lactic acid bacteria, lactobacillus pentosus strain b240 (b240) has the highest iga-inducing potency in mouse peyer's patch cells. we previously reported a practical new finding that oral ingestion of nonviable heat-killed b240 ... | 2010 | 21095259 |
| phenotypic and genotypic characterization of influenza virus mutants selected with the sialidase fusion protein das181. | influenza viruses (ifvs) frequently achieve resistance to antiviral drugs, necessitating the development of compounds with novel mechanisms of action. das181 (fludase), a sialidase fusion protein, may have a reduced potential for generating drug resistance due to its novel host-targeting mechanism of action. | 2010 | 21097900 |
| passive immune-prophylaxis against influenza virus infection by the expression of neutralizing anti-hemagglutinin monoclonal antibodies from plasmids. | the genetic delivery of therapeutic monoclonal antibodies (mabs) by in vivo production may offer a new solution to the current problems in the mab therapy for microbial diseases. herein, plasmids encoding the neutralizing mab against hemagglutinin (ha) of a/pr/8/34 influenza virus (ifv) were electro-transferred into mouse muscle and the relationship between serum recombinant anti-ha mab (rha mab) levels and the prophylactic efficacy against lethal ifv infection were analyzed. pretreatment of the ... | 2011 | 21266754 |
| a safety evaluation of das181, a sialidase fusion protein, in rodents. | das181 is a novel inhaled drug candidate blocking influenza virus (ifv) and parainfluenza virus (piv) infections through removal of sialic acid receptors from epithelial surface of the respiratory tract. to support clinical development, a 28-day glp inhalation toxicology study was conducted in sprague-dawley rats. in this study, achieved average daily doses based on exposure concentrations were 0.47, 0.90, 1.55, and 3.00 mg/kg/day of das181 in a dry powder formulation. das181 was well tolerated ... | 2011 | 21572096 |
| [characteristics of human metapneumovirus respiratory tract infection in children and the relationship between the infection and meteorological conditions]. | to study the epidemiology of human metapneumovirus (hmpv) infection in children and its relations with meteorological conditions in suzhou. | 2011 | 21575373 |
| oral administration of bifidobacterium longum ameliorates influenza virus infection in mice. | we investigated whether the oral administration of bifidobacterium longum bb536 could ameliorate influenza virus (ifv) infection in a mice model. mice were orally administrated bb536 or saline for 2 weeks and then infected with ifv. orally administered bb536 significantly alleviated symptoms, reduced the loss of body weight, and inhibited viral proliferation in the lungs relative to the control group findings. histopathological findings in the lungs were improved in the bb536 group compared to c ... | 2011 | 21804232 |
| Adenovirus-Vectored Drug-Vaccine Duo as a Rapid-Response Tool for Conferring Seamless Protection against Influenza. | Few other diseases exert such a huge toll of suffering as influenza. We report here that intranasal (i.n.) administration of E1/E3-defective (+öE1E3) adenovirus serotype 5 (Ad5) particles rapidly induced an anti-influenza state as a means of prophylactic therapy which persisted for several weeks in mice. By encoding an influenza virus (IFV) hemagglutinin (HA) HA1 domain, an Ad5-HA1 vector conferred rapid protection as a prophylactic drug followed by elicitation of sustained protective immunity a ... | 2011 | 21818346 |
| genome sequence of seiv-1, a novel virus from the iflaviridae family infective to spodoptera exigua. | analysis of the transcriptome of spodoptera exigua larvae revealed the presence of several ests with homology to virus of the order picornavirales and with the highest similarity to infectious flacherie virus (iflaviridae) that infects bombyx mori larvae. iflaviridae is a recently defined family of insect-infecting viruses that consist of positive single strand rna genomes translated into a single polyprotein of around 3000 amino acids long. using the sequence information derived from the obtain ... | 2012 | 22041201 |
| high-resolution computed tomography findings of influenza virus pneumonia: a comparative study between seasonal and novel (h1n1) influenza virus pneumonia. | purpose: to evaluate the high-resolution computed tomography (hrct) findings of novel influenza virus (n-ifv) pneumonia and compare them with the findings for seasonal (s-ifv) pneumonia. materials and methods: we evaluated 29 cases of pure ifv pneumonia that occurred between 1990 and 2010. we evaluated the existence, extent, and patterns of hrct findings and compared these features between s-ifv and n-ifv. ... | 2011 | 22180185 |
| oral administration of patchouli alcohol isolated from pogostemonis herba augments protection against influenza viral infection in mice. | seasonal influenza a infection results in considerable morbidity and mortality. the limited efficacy of available therapeutic strategies stresses the need for development and study of new molecules against influenza virus (ifv). patchouli alcohol (pa), the major chemical constituent of pogostemonis herba, was previously found to strongly inhibit influenza h1n1 replication in vitro. in the present study, the in vivo anti-ifv effect of pa was investigated. in a mouse model infected with lethal lev ... | 2011 | 22193241 |
| lactobacillus plantarum strain yu from fermented foods activates th1 and protective immune responses. | lactic acid bacteria (lab) are known to have effects on immune function. from 203 strains of lab isolated from fermented foods, we selected a beneficial strain, lactobacillus plantarum strain yu (lpyu), which has high interleukin (il)-12-inducing activity in mouse peritoneal macrophages. this activity of lpyu was partially mediated by toll-like receptor (tlr) 2, but not tlr4 or tlr9. oral administration of lpyu to ovalbumin (ova)-immunized mice caused suppression of serum ova-specific immunoglob ... | 2011 | 21893216 |
| efficacy of oral administration of heat-killed probiotics from mongolian dairy products against influenza infection in mice: alleviation of influenza infection by its immunomodulatory activity through intestinal immunity. | some probiotics possess immunomodulatory activities and have been used as complementary and alternative medicines. we previously found that 10 lactic acid bacteria (lab) strains isolated from traditional mongolian dairy products showed probiotic potential in vitro. in this study, we assessed the immunomodulatory activity of 10 labs on influenza virus (ifv) infection in relation to their efficacies in ifv-infected mice. in an intranasal ifv infection model in mice, oral administration of boiled l ... | 2011 | 21871585 |
| quantitative analysis of the lipidomes of the influenza virus envelope and mdck cell apical membrane. | the influenza virus (ifv) acquires its envelope by budding from host cell plasma membranes. using quantitative shotgun mass spectrometry, we determined the lipidomes of the host madin-darby canine kidney cell, its apical membrane, and the ifv budding from it. we found the apical membrane to be enriched in sphingolipids (sps) and cholesterol, whereas glycerophospholipids were reduced, and storage lipids were depleted compared with the whole-cell membranes. the virus membrane exhibited a further e ... | 2012 | 22249292 |
| ictv virus taxonomy profile: iflaviridae. | iflaviridae is a family of small non-enveloped viruses with monopartite, positive-stranded rna genomes of approximately 9-11 kilobases. viruses of all classified species infect arthropod hosts, with the majority infecting insects. both beneficial and pest insects serve as hosts, and infections can be symptomless (nilaparvatalugens honeydew virus 1) or cause developmental abnormalities (deformed wing virus), behavioural changes (sacbrood virus) and premature mortality (infectious flacherie virus) ... | 2017 | 28382900 |
| proteomic analysis of silkworm midgut cellular proteins interacting with the 5' end of infectious flacherie virus genomic rna. | the flacherie disease in the silkworm is caused by the infectious flacherie virus (ifv). ifv relies on its 5' region of genomic rna to recruit host-related factors to implement viral translation and replication. to identify host proteins bound to the 5'-region of ifv rna and identify proteins important for its function, mass spectrometry was used to identify proteins from silkworm midgut extracts that were obtained using rna aptamer-labeled 5' region of ifv rna. we found 325 protein groups (uniq ... | 2015 | 25534780 |
| rapid detection of infectious flacherie virus of the silkworm, bombyx mori, using rt-pcr and nested pcr. | in this study, a method for detection of an ssrna viral pathogen that causes viral flacherie in the silkworm, bombyx mori (l.) (lepidoptera: bombycidae), was used for the detection of b. mori infectious flacherie virus (bmifv). a combination of nested and reverse transcriptase polymerase chain reaction was used for detection. although bmifv has been reported in almost all the sericultural regions of the world, there had been no reports of bmifv incidence in india. therefore, the confirmation of ... | 2013 | 24785655 |
| detection of an internal translation activity in the 5' region of bombyx mori infectious flacherie virus. | the 5' untranslated region plays an important role in positive-sense single-stranded rna virus translation initiation, as it contains an internal ribosome entry site (ires) that mediates cap-independent translation and is applied to simultaneously express several proteins. infectious flacherie virus (ifv) is a positive-sense single-stranded rna virus; however, the ires function is still not proved. to investigate whether the sequences of ifv contain ires activity, a series of bicistronic reporte ... | 2012 | 22476262 |