| standardized method of sendai virus production for biological assays. | | 1976 | 11671 |
| relationships between chemical structure and antiviral activity of some biguanide derivatives. | the aim of this report was to indicate the correlation between chemical structure and antiviral activity of some biguanide derivatives obtained according to monsanto chemicals ltd. neth. appl. 289. 283. the studies were carried out on the culture of monkey kidney cells infected with parainfluenza sendai virus and on the mice infected with influenza virus. the increase of antiviral activities of n-phenylbiguanide and n-p-ethoxyphenylbiguanide was estimated. | 1977 | 14461 |
| physicochemical properties of sendai virus rna. ii. effect of ionic strength on thermostability of rna. | the dependence of the melting point (tm) and width of the melting range (deltatm) on the ionic strength and ph of the medium was investigated for the double-stranded rna formed through self-hybridization during the isolation of rna from sendai virus. it was shown that tm is a linear function of the logarithm of the sodium ion concentration in the range of concentrations from 10(-1) to 10(-4) m, with a slope of 11.5 degrees toward the abscissa for each order of magnitude. the width of the melting ... | 1976 | 15210 |
| cellular proteases increased in paramyxovirus (sendai virus) carrier cells possibly responsible for enhanced formation of cowpox virus-specific cell surface antigen. | cowpox virus (cpv) growth and its s-ag (cell surface antigen) formation in hvj (sendai virus) carrier cells pre-treated with actinomycin d or puromycin were not affected as much as those in parent cells. these suggest the different cellular functions of carrier cells. the activity of carrier cell extracts causing a characteristic degradation of cpv reacted with them in vitro disappeared after the pre-incubation of extracts with hemoglobin or casein. measurements of cellular protease activities ... | 1977 | 15532 |
| influence of the isolation procedure on the structure, neuraminidase and heamagglutinating activities of sendai virus envelope glycoproteins. | the influence of the isolation procedure of sendai virus glycoproteins from the virus surface on their neuraminidase and haemagglutinating activities, as well as on some structural properties was studied. these glycoproteins exhibited lower specific neuraminidase and haemagglutinating activities than those of intact virus. neuraminidase activity was expressed when the glycoproteins were constituted in forms with molecular weights of about 70,000 (monomer), 160,000 (dimer), 300,000 (tetramer) or ... | 1978 | 35940 |
| specific alteration of sendai virus glycoprotein subunits. | the alteration produced by chemical and physical agents in the structure and neuraminidase activity of sendai virus glycoproteins was studied. while dissociation of glycoproteins with 1% sodium dodecyl sulphate (sds), 2% beta-mercaptoethanol and 5 m urea for 2 min at 100 degrees c yielded the known hn and f subunits (mol. wts. 60,000 and 53,000), in the presence of 1% sds the glycoproteins were converted into components with approximate mol. wts. of 60,000, 120, 000 and higher. treatment of the ... | 1979 | 35955 |
| nucleologenesis in chick erythrocyte nuclei reactivated by cell fusion. | | 1979 | 40045 |
| sendai virus multiplication in the presence of ceruloplasmin and homologous virus envelopes. | preincubation of sendai virus with ceruloplasmin induced in chorioallantoic membrane (cam) fragments the synthesis of a population with decreased infectivity and high haemagglutinating (ha) and neuraminidase (na) activities, whereas preincubation with solubilized virus envelopes led to the production of a viral population with the same infectivity as the control, but with lower ha and na activities. preincubation of the virus with a mixture of ceruloplasmin and solubilized envelopes depressed th ... | 1979 | 41434 |
| a replication defective mutant of rous sarcoma virus which fails to make a functional reverse transcriptase. | | 1975 | 46651 |
| tumor-associated transplantation antigens in immune rejection of mouse malignant cell hybrids. | the inability of mouse cell hybrids derived from two malignant parental cells to produce tumors in syngeneic f1 hosts was analyzed. yc hybrids, derived from the fusion of c1.1d and l1210 clls, failed to induce any tumor in adult mice, while 91% of x-irradiated newborn mice developed tumors and died. some telocentric chromosomes were lacking in hybrid tumors; however, none of the immunologically intact adult mice developed tumors when grafted with tumors grown in x-irradiated newborn mice. this i ... | 1975 | 49053 |
| two antigenically distinct species of human interferon. | rabbit antisera prepared against interferon produced in human fibroblast cell cultures stimulated with poly(1).poly(c) neutralized the activity of interferon preparations produced in various human fibroblast cultures timulated either with poly(1)poly)c) or with viruses. however, these antisera showed no detectable neutralizing activity against interferon produced in cultures of human leukocytes. on the other hand, most rabbit antisera against the human leukocyte interferon were active in neutral ... | 1975 | 49055 |
| early sterile autopsy in etiological studies on multiple sclerosis. | the clinical findings and course in the first 2 cases of multiple sclerosis are described, in whom it was possible to isolate a virus from brain tissue by early sterile autopsy and fusion technique. it is noteworthy that in one of these cases multiple sclerosis probably occurred in female members of the family through three consecutive generations. a report is made on an additional case, in which no virus could be isolated, but in which electron microscopic studies showed two types of virus part ... | 1975 | 49403 |
| multiple sclerosis and parainfluenza 1 virus. history of the isolation of the virus and expression of phenotypic differences between the isolated virus and sendai virus. | 54 cultures were established from brain tissue obtained 2-3 hrs after death from 1 case of multiple sclerosis and 30 cultures from another case. following fusion with indicator cells in the presence of lysolecithin, a parainfluenza type 1 virus (6/94 virus) was isolated from cultures representing one plaque area in the first case and one plaque area in the second case. a cell line chronically infected with the 6/94 virus has been maintained for more than 100 passages in vitro. a close relationsh ... | 1975 | 49404 |
| antigenic variation among parainfluenza type 1 (sendai) viruses: analysis of 6/94 virus. | 6/94 virus, isolated originally from a multiple sclerosis (ms) patient, was compared antigenically with related parainfluenza type 1 strains. these included two sendai strains of mouse and two sendai strains of reported human origin as well as the ha2 strain. by standard hemagglutination inhibition (hi) or hemadsorption neutralization (had-n) tests or by the complement-fixation (cf) cross-block titration test for detecting surface antigens, 6/94 virus and the sendai virus strains were indistingu ... | 1975 | 52630 |
| letter: lymphocyte agglutination in multiple sclerosis. | | 1975 | 52801 |
| cell-mediated cytotoxicity against murine cells infected with 6/94 virus, a parainfluenza type 1 isolate from multiple sclerosis brain tissue. | maximum cytotoxicity to parainfluenza type 1 viral determinants occurs on day 5 post-immunization in mice with and without preexisting anti-6/94 virus antibody. animals with preexisting anti-6/94 serum antibody, however, exhibited a markedly higher cytotoxic response. syngeneic, allogeneic, and xenogeneic virus-infected cells induce virus-specific cytotoxicity, but only infected cells histocompatible with the effector cells can serve as susceptible target cells. the effector cell is a t cell, an ... | 1976 | 57926 |
| on the study of sendai virus hemolysis. i. complete sendai virus lacking in hemolytic activity. | | 1976 | 58472 |
| on the study of sendai virus hemolysis. ii. morphological study of envelop fusion and hemolysis. | | 1976 | 58473 |
| glycoproteins of sendai virus: purification and antigenic analysis. | solubilized envelope antigens (glycoproteins) of sendai virus were purified in a deae-bio-gel a column. monospecific antisera were prepared against the antigens, designated hn and f glycoproteins. these glycoproteins are antigenically distinct. immunodiffusion analyses of the envelope antigens of three representative paramyxoviruses (sendai, yucaipa, and newcastle disease virus)did not reveal any cross-reactions among them with antisera specific for sendai virus glycoproteins. | 1975 | 58850 |
| cell-mediated cytotoxicity against sendai-virus-infected cells. | after injection of sendai virus, a parainfluenza virus type i, mice generate cytotoxic lymphocytes which lyse specifically sendai-virus-infected target cells in vitro. their action is not inhibited by specific antibody in vitro. killer cell activity appears 4 days after infection, reaches a maximum on the 7th day and disappears on the 14th to 16th day. decrease of cytotoxic cell activity is correlated with an increase of haemagglutinating antibodies. the cytotoxic effector cell could be characte ... | 1976 | 60844 |
| shared determinants between virus-infected and trinitrophenyl-conjugated h-2-identical target cells detected in cell-mediated lympholysis. | infection of h-2-identical mice with either lymphocytic choriomeningitis (lcm) virus, vaccinia virus, or paramyxo (sendai) virus resulted in the generation of specifically sensitized cytotoxic t lymphocytes (ctl). ctl generated in vitro against 2,4,6-trinitrophenyl (tnp)-conjugated syngeneic stimulator cells were specifically cytotoxic for tnp-conjugated h-2k (d) region identical targets. both lcm and vaccinia-induced ctl, however, were found to be strongly cytotoxic towards tnp-conjugated, h-2k ... | 1976 | 63379 |
| elisa, a viral diagnostic method. | | 1977 | 66489 |
| enhancement of ige-mediated histamine release from human basophils by viruses: role of interferon. | human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen e or anti-ige. leukocyte cultures incubated with virus (i.e. hsv-1, influenza a, and adeno-1) but not exposed to ragweed antigen e or anti-ige fail to release histamine. if, however, leukocyte cultures are first exposed to virus and then to ragweed antigen e or anti-ige, significant enhancement of histamine release occurs. both infectious and inactivated virus enhance histamine release and the ... | 1977 | 67173 |
| enhancement and stabilization effect of edta on sendai virus neuraminidase activity. | edta was found to enhance, and exert a stabilizing effect on, sendai virus neuraminidase activity. the effect was dependent on the molecular form of the enzyme and on the nature of the substrate. the "native form" (enzyme bound to the virions) was less influenced than the "mixed form" (enzyme separated from the surface of virions together with other glycoproteins), which in turn was less influenced than the purified enzyme. on the other hand, the stimulation by edta of neuraminidase-induced hydr ... | 1977 | 71853 |
| immunologic properties of purified sendai virus glycoproteins. | | 1977 | 72108 |
| fusion of human cells with haplopappus protoplasts by means of sendai virus. | | 1977 | 73530 |
| histamine release from rat mast cells induced by sendai virus. | | 1977 | 74020 |
| involvement of fusion activity of ultraviolet light-inactivated sendai virus in formation of target antigens recognized by cytotoxic t cells. | mice inoculated with ultraviolet light-inactivated sendai virus mount a cell- mediated immune response to the virus. cytotoxic t cells specific for sendai virus can be obtained by in vitro secondary stimulation of primed spleen cells with syngeneic stimulator cells coated with uv-inactivated sendai virus. neither in vivo nor in vitro stimulation alone is sufficient to generate specific cytotoxic t cells. sharing of the h-2 haplotype between cytotoxic t cells and target cells is required for the ... | 1978 | 78960 |
| viral infections and ige levels. | six non-atopic patients with respiratory infections to a variety of viruses demonstrated a consistent drop in ige levels (35% to 87%) in the convalescent phase compared to the acute phase of infection, suggesting that the viral agent affects t lymphocytes to suppress and hence b lymphocytes to secrete ige immunoglobulin. | 1978 | 80144 |
| epidemiology of respiratory viral infection among paediatric inpatients over a six-year period in north-east england. | during a 6-year, hospital-based study at newcastle upon tyne five consecutive winter epidemics of respiratory syncytial (r.s.) virus infection occurred; the virus was identified in 1428 cases, showing that 1 in 50 live-births were admitted to hospital with r.s. virus infection. epidemics were inversely related to temperature and to number of hours of sunshine. parainfluenzaviruses, the second largest group of pathogens, were identified in 543 cases; most infections by this group were due to para ... | 1978 | 82045 |
| primary in vitro sensitization of virus specific cytotoxic t lymphocytes. | cba mouse-derived splenic lymphocytes treated with either beta-priopionlactone-inactivated or u.v. light-inactivated parainfluenza (sendai) virus stimulated in vitro unprimed syngeneic t-lymphocytes to differentiate into cytotoxic t-lymphocytes (ctl). the ctl generated were virus specific and h-2 restricted. for optimal ctl responses to be induced (i) a critical treatment of stimulator cells, (ii) an optimal ratio of responder to stimulator cells and (iii) an in vitro incubation period of 5 days ... | 1978 | 82534 |
| h-2-linked murine cytotoxic t cell responses specific for sendai virus-infected cells. | cba (h-2k) mouse-derived lymphochoriomeningitis virus and herpes simplex virus-specific cytotoxic t lymphocytes lyse virus-infected target cells compatible on either the h-2k or h-2d region. in contrast, cba, c3h and akr (h-2k) mouse-derived sendai virus-specific cytotoxic t lymphocytes (ctl) fail to lyse h-2d-compatible virus-infected cells. a similar lack of h-2d region-associated lytic activity was found with c57bl/6 and c57bl/10 (h-2b) mice as well as with the recombinants b10.a (2r) [kb-db] ... | 1978 | 83237 |
| generation on infected fibroblasts of human t and non-t lymphocytes with specific cytotoxicity, influenced by histocompatibility, against measles virus-infected cells. | | 1979 | 87462 |
| generation of virus-specific cytotoxic t cells in vitro. i. induction conditions of primary and secondary sendai virus-specific cytotoxic t cells. | h-2-restricted cytotoxic t cells specific for sendai virus were generated in vitro in a primary response from normal mouse lymphocytes cultured in the presence of infective as well as inactivated sendai virus. antigen-presenting cells of different origin, including t cells, were found to be effective stimulators. antibodies to sendai virus were shown to inhibit the activation of specific precursor killer cells when added to cultures before, but not after, the addition of viral antigen. data obta ... | 1979 | 92413 |
| characteristics and genetic control of nk-cell-mediated cytotoxicity activated by naturally acquired infection in the mouse. | most and perhaps all natural-cell-mediated cytotoxicity (ncmc) may be activated by a response to exogenous infections. specific-pathogen-free (spf) mice have little or no natural killer (nk) cell activity, but when they are relocated in conventional conditions, they develop strong ncmc within 2 to 3 days. unlike other spf animals, hypothymic nude mice display good ncmc which is further augmented upon their entry into a pathogenic environment. the ontogeny, genetic control and other features of p ... | 1979 | 93585 |
| modulation by cell trypsinization of sendai virus expression in african green monkey kidney cells: first infection and establishment of a carrier state. | two african green monkey kidney (agmk) cell lines, 37rc (interferon-producing) and vero (non-interferon-producing), were infected by egg-grown sendai virus passaged in eggs at high and low m.o.i. the appearance of haem-adsorption, and cytopathic effect (c.p.e.) as well as the presence of haemagglutinating virions in the supernates were much more pronounced with a virus seed obtained with 10(-3) diluted passages than with a seed obtained with undiluted inoculum. they were also independent of inte ... | 1979 | 94347 |
| different sensitivity of hemagglutinating and hemolytic activities of sendai virus to non-antibody inhibitors. | research was carried out in order to verify if some components of human serum, which are capable of inhibiting the hemagglutination by sendai virus, are active even towards the hemolysis by the same virus. it was ascertained that both orosomucoid and low-density lipoproteins obtained by floatation had no action on the hemolysis by sendai virus while lipoproteins precipitated with dextrane sulphate showed a certain inhibiting effect. this action however is not due to lipoproteins but is probably ... | 1979 | 95432 |
| contributions of cytoplasmic factors to in vitro cellular senescence. | mass populations of normal human lung fibroblasts were enucleated by centrifugation at greater than or equal to 25,000 g in 4 mug/ml cytochalasin b. the 1 per cent of cells that did not enucleate where rendered nonviable by treatment with mitomycin c. whole cells were poisoned with a 99 per cent lethal dose of the sulfhydryl reagent iodoacetate. the washed cells were then mixed with the anucleate cytoplasms, fused with inactivated sendai virus, and planted in rotenone for 20 hours. whereas norma ... | 1975 | 162798 |
| temperature-sensitive virus derived from bhk cells persistently infected with hvj (sendai virus). | bhk-hvj cells, a cell line of baby hamster kidney cells persistantly infected with hvj (sendai virus), started to produce infectious virus by shifting down the incubation temperature from 38 to 32 c. the virus derived from bhk-hvj cells, designated as hjv-pb, was effectively neutralized with antibody against wild-type virus (hvj-w) which was used for the establishment of bhk-hvj cells. hvj-pb replicated in eggs at 32 c, but not at 38 c, while hvj-w grew equally well at both temperatures. when bh ... | 1975 | 163346 |
| malignant transformation of hamster kidney cells by bk virus. | primary hamster kidney cells were transformed by bk virus, a new human papovavirus. transformed (hkbk) cell produced bk virus t antigen and induced tumors in hamsters that developed antibodies to bk virus t antigen. bk virus was rescued from hkbk cells by sendai virus-assisted fusion with permissive cells. one out of six cell lines derived from hkbk cell-induced tumors showed the same characteristics as hkbk cells. | 1975 | 163364 |
| [effect of rous sarcoma virus on the acid phosphatase activity in cells sensitive and naturally resistant to the virus]. | | 1975 | 163552 |
| detection by means of cell fusion of macromolecular synthesis involved in the reconstruction of the nuclear envelope in mitosis. | using the cultured chinese hamster cell line don, g1 or s or a mixture of late-s/g2 cells were prepared by release from metaphase arrest. metaphase (m) cells were also obtained by mitotic arrest of log-phase cultures with colcemid and held in metaphase; such m cells remained untreated with any other compound and were termed standard m cells. when interphase (i) cells were fused at ph 8.0 and 37 degrees c with standard cells in the presence of colcemid by means of uv-inactivated sendai virus, bin ... | 1975 | 163831 |
| genetic heterogeneity in xeroderma pigmentosum: complementation groups and their relationship to dna repair rates. | fibroblast strains from 12 patients with xeroderma pigmentosum had lower than normal rates of dna repair, as determined by autoradiographic studies of ultraviolet-induced unscheduled nuclear dna synthesis. the nuclei in binuclear cells, obtained by fusing fibroblasts from certain pairs of these strains, had a greater rate of dna repair than the nuclei of either strain's unfused mononuclear cells. these results indicate that complementary corrections of the strains' repair defects had occurred in ... | 1975 | 164028 |
| differentiation in erythroleukemic cells and their somatic hybrids. | clones of erythroleukemic cells differ in the extent to which they (1) undergo differentiation spontaneously and (2) can be induced to differentiate in the presence of dimethylsulfoxide. here we demonstrate that relative differences in globin gene expression within and between clones largely reflect differences in the proportion of cells participating in differentiation rather than uniform differences in the extent to which all cells in these clones undergo differentiation. we call this phenotyp ... | 1975 | 164031 |
| assembly of viruses. | | 1975 | 164048 |
| do hyperplastoid cell lines "differentiate themselves to death"? | | 1975 | 164106 |
| prematurely condensed chromosomes of dividing and non-dividing cells in aging human cell cultures. | | 1975 | 164354 |
| mammalian cell fusion. vi. regulation of mitosis in binucleate hela cells. | | 1975 | 164357 |
| identification of sendai virus neuraminidase and hemagglutinin subunits. | | 1975 | 164379 |
| detection of cellular hypersensitivity among multiple sclerosis (ms) patients to 6/94 virus; a parainfluenza type 1 isolate from ms brain tissue. | multiple sclerosis (ms) patients and normal subjects were tested for cellular hypersensitivity, using the leukocyte migration inhibition test lmi), to a parainfluenza-1-virus (6/94), previously isolated from brain biopsy tissue of an ms patient. the response of ms patients as a group was not greater than that of normals to 6/94, but a significant increase in the response was observed among selected patients in the early active and chronic advanced stages of the disease. ms patients, however, did ... | 1975 | 164416 |
| neoplastic differentiation: interaction of simian virus 40 and polyoma virus with murine teratocarcinoma cells in vitro. | the host-virus interactions of simian virus 40 (sv40) and polyoma virus (py) with cell lines established from a teratocarcinoma were studied. the cells utilized in this study were the multipotential stem cell of the teratocarcinoma, embryonal carcinoma, and differentiated cells derived from embryonal carcinoma. several lines of differentiated cells were established in vitro which included parietal yolk sac, epithelial, and spindle cell types. embryonal carcinoma cells are not susceptible to infe ... | 1975 | 164473 |
| distinction between malignant l cells and normal mouse fibroblasts by rosette formation with sheep red blood cells. | murine l cell fibroblasts, and derivatives were found to rosette with sheep red blood cells (srbc). primary fibroblast explants from the parent murine strain, c3h, did not possess this potential. no rosettes were observed with primary fibroblast explants from c57bl and b10br mice, with a human fetal lung fibroblast, with baby hamster fibroblasts or their polyoma transormed derivative, or with a cell line, 1t-22, derived from balb/c mice. hybridization of 1t-22 and l cells, by sendai virus-mediat ... | 1975 | 164508 |
| selective thermal effects on freeze-dried biological materials. | | 1975 | 165039 |
| contribution to the study of the parainfluenza antigens. | from epidemiological data it appeared that an epidemic of parainfluenza type 1 occurred in lyons in october-november 1967. thereafter a few sporadic cases were detected. then in 1970 strains were isolated which showed a change in the hemagglutinin antigen. the persistence of antibodies in the population is shorter than that of the other parainfluenza viruses (i. e. parainfluenza 3); it is not known if reinfections with parainfluenza 1 occur since there are cross-reactions by cf test between para ... | 1975 | 165116 |
| vaccination against parainfluenza 1 virus (typus muris) infection in order to eradicate this virus in colonies of laboratory animals. | parainfluenza 1 virus (typus muris), commonly known as sendai virus, is still contaminating mouse colonies in japan. this presents a serious problem to keep mouse colonies pathogen-free. it seems that the infection is spread by dust and the virus remains active a considerable time in the mouse breeding rooms after all mice are removed. in order to eradicate the infection of this virus, vaccination was attempted to inoculate newly born young mice successively for about six months and during and a ... | 1975 | 165125 |
| organ cultures of rat and hamster colon. | human fibrin foam as a matrix for three-dimensional organ cultures was successfully employed for the cultivation of newborn rat and hamster colon tissue. colonic tissue was maintained under different oxygen atmospheres and with various synthetic media. explants of rat and hamster colon, maintained in leibovitz's l-15 medium buffered with hepes in the presence of 5% co2 and air, retained their normal columnar epithelial architecture for 24 days in culture. outgrowth of epithelial cells from colon ... | 1975 | 165141 |
| sarcoma in a hamster inoculated with bk virus, a human papovavirus. | an undifferentiated sarcoma occurred in 1 of 52 hamsters inoculated when newborn with bk virus (bkv), a simian virus 40 (sv40)-related human papovavirus. it was transplantable and grew in tissue culture. sera of tumor-bearing hamsters were without antibodies reactive to bkv virion antigens in hemagglutination-inhibition and neutralizationtexts, but contained antibodies reactive in immunofluorescence (if) tests to sv40 t antigen in sv40-transformed cells and to antigens in cells acutely infected ... | 1975 | 165302 |
| polyoma virus-transformed rat cell lines inducible for viral capsid antigen synthesis. | | 1975 | 165623 |
| routes of fetal infection and mechanisms of fetal damage. | | 1975 | 165711 |
| hydrocephalus produced by the 6/94 virus; a parainfluenza type 1 isolate from multiple sclerosis brain tissue. | the 6/94 virus, parainfluenza type 1 isolate from multiple sclerosis brain tissue, produced hydrocephalus in newborn syrian hamsters. all animals developed clinical disease and died within a week. ependymal cells lining the aqueduct of sylvius became necrotic and fused, resulting in obstructive hydrocephalus. the 6/94 virus antigen was seen in ependyma and meninges. paramyxovirus nucleocapsids were seen within cytoplasm of ependymal cells. virus was recovered from hamster brains for only two day ... | 1975 | 165802 |
| studies on the immune response and pathogenesis of sendai virus infection of mice. iii. the effects of cyclophosphamide. | studies of the effects of cyclophosphamide on a non-lethal primary sendai virus infection of mice are reported. treatment with cyclophosphamide resulted in failure to limit or eradicate virus, diminished and delayed the appearance of immunoglobulin-secreting cells in the lungs and the production of local and serum antibody, reduced and delayed the appearance of bronchial basement membrane damage and the desquamation of infected mucosal cells, and reduced the incidence of immune complex depositio ... | 1975 | 166035 |
| complementing xeroderma pigmentosum fibroblasts restore biological activity to uv-damaged dna. | uv survival curves of adenovirus 2 using fused, complementing xeroderma pigmentosum (xp) fibroblast strains as virus hosts showed a component with an inactivation slope identical to that given by normal cells. this component was not observed when the fibroblasts were not fused or when fusion involved strains in the same complementation group. extrapolation of this component indicated that at zero dose 3% of the viral plaque-forming units had infected cells capable of normal repair. these results ... | 1975 | 166304 |
| comparative study of the protein kinase associated with animal viruses. | | 1975 | 166492 |
| antigenomes in sendai virions and sendai virus-infected cells. | | 1975 | 166498 |
| phagocytosis of lymphoblastoid cells and cell destruction of human malignant tumor cells. | on the basis of the previous study, on the cell interaction between malignant tumor cells and other cells, especially with lymphocytes, the present study was carried out by investigating cell to cell interaction of human malignant tumor cells and human lymphoblastoid cells such as t-cell (molt-4 cell) and b-cell (burkitt lymphoma cell). as a result it has been revealed that live lymphoblastoid cells were not adhered on the cell surface of the tumor cells, nor is it ingested by tumor cells, but i ... | 1975 | 166555 |
| failure of attenuated temperature-sensitive influenza a (h3n2) virus to induce heterologous interference in humans to parainfluenza type 1 virus. | the present investigation was undertaken to determine if a candidate live vaccine virus, influenza a/hong kong/68-ts-1 [e] (h3n2), induced heterologous interference against an interferon-sensitive, wild-type, parainfluenza type 1 challenge virus. the parainfluenza virus was administered 7 days after hong kong/68-ts-1 [e] virus infection. the clinical response, daily quantitative virus shedding, interferon production, and serum and nasal wash antibody responses were determined in an experimental ... | 1975 | 166929 |
| studies on cell fusion between babesia rodhaini-infected mouse erythrocytes and baby hamster kidney cells. | | 1975 | 166951 |
| "ultramicroinjection" of macromolecules or small particles into animal cells. a new technique based on virus-induced cell fusion. | a new method is described for the introduction of macromolecules and small particles into animal cells. the first step in this procedure is the trapping of particles in ghosts of human erythrocytes. this is achieved by the gradual hemolysis of erythrocytes in the presence of the particles to be trapped. the second step is the sendai virus-induced fusion of the ghosts containing the particles with cells. by this method, ferritin and latex spheres (diameter 0.1 mum) have been "injected" into cells ... | 1975 | 167032 |
| iterferon induction in mice by bhk cells persistently infected with hvj. | intraperitoneal injection into mice of bhk-hvj cells (bhk cells persistently infected with hvj) induced considerable amounts of circulating interferon. sonication of bhk-hvj cells almost totally abolished their interferon inducing capacities. this result suggests that a certain native organized structure of virus infected cells might be essential for production of interferon in this system, as is suggested in the in vitro sytem. | 1975 | 167109 |
| location and abundance of poly (a) sequences in sendai virus messenger rna molecules. | adenine-rich sequences from 18s sendai virus messenger rna species were 99% adenylate, 3'-oh terminal, and were present in at least 50% of the rna molecules. intact virus messenger rna molecules were resistant to exonucleolytic attack by polynucleotide phosphorylase, suggesting that their 3'-termini are masked. | 1975 | 167113 |
| protein synthesis in sendai virus-infected cells. | the rate of protein synthesis in chicken embryo cells infected with sendai virus 18 to 20 h previously was about two times greater than in mock-infected controls. at this time of infection six stable virus-induced proteins, four major structural proteins (p, nh, np and m) and two non-structural proteins (28k and 61k), were identified by electrophoresis in sds-polyacrylamide gel of total cell extracts. the structural glycopeptide f was not detected in the infected cell extracts. pulse-chase exper ... | 1975 | 167115 |
| husbandry of the "nude" mouse in conventional and germfree environments. | the "nude" mouse is a unique tool for immunologic studies. its relatively short life span dictates the application of rigid environmental controls to increase longevity if the mouse is to assume the role of a practical experimental animal. in this paper we discussed the husbandry procedures employed to raise "nude" mice in our facilities under conventional, defined flora, and germfree conditions. conventional and defined flora mice were raised on laminar flow stay-clean rocks, and germfree "nude ... | 1975 | 167230 |
| experimental parainfluenza-type-1-virus-induced encephalopathy in the adult mouse. an ultrastructural study of early lesions. | the adult mouse inoculated intracerebrally with 6/94 strain of parainfluenza type 1 virus developed selective degenerative lesions in cerebral white matter. ultrastrucrally, the infiltration of mononuclear cells, mostly lymphoid cells, apparently preceded the alterations of white matter parenchyma. the prominent feature of the white matter lesion was a lytic degeneration of both axon and myelin that seemed to be triggered by the mononuclear cell infiltration. nucleocapsids of paramyxovirus were ... | 1975 | 167587 |
| inhibition of the growth of hemadsorption 2 virus by three acyl derivatives of amino acids. | three acyl derivatives of amino acids, dicarbobenzoxy-l-lysine sodium, carbobenzoxy-l-aspartic acid-beta-benzyl ester potassium, and n-3-phenylpropionyl-s-benzyl-l-cysteine potassium inhibit the growth of parainfluenza 1 (hemadsorption 2) virus. the growth of simian virus 40, vaccinia, poliomyelitis type 1, semliki forest, eastern equine encephalitis, and western equine encephalitis viruses was not affected by these compounds. four other acyl derivatives of amino acids did not inhibit the growth ... | 1975 | 167659 |
| an immunoprecipitin study of the incidence of influenza a antibodies in animal sera in the ottawa area. | a survey of over 600 'normal' sera from 14 animal species by immunoprecipitin tests in cellulose acetate using viron antigens revealed a high incidence of precipitating activity against a broad range of influenza a virus strains, particularly a2hhong kong/1/68 and /pr8. however, serum treatments trypsin-heat-periodate, naio4, v. cholerae receptor-destroying enzyme (rde), or kaolin eliminated most precipitating activity, which suggests that it was due to "non-specific" inhibitors of influenze vir ... | 1975 | 167931 |
| [proceedings: in vitro cultivation of ehrlichascites tumor cells by cell fusion]. | | 1975 | 168136 |
| heterokaryons in the analysis of genes and gene regulation. | cytological and chemical analysis of heterokaryons, the immediate product of cell fusion, offer new possibilities for studying the factors responsible for genetic regulation in eukaryotic cells. in comparison with proliferating cell hybrids the heterokaryon state offers the important advantage that a heterokaryon contains two complete genomes since chromosome loss does not occur, but since segregation and recombination are absent, heterokaryons cannot be used for gene mapping in the same way as ... | 1975 | 168148 |
| specific macrophage immunity to sendai virus: macrophage aggregation in vitro with sendai virus by cytophilic antibodies. | when a 24-h tube culture of rabbit alveolar macrophages was infected with sendai virus, the rate of infected cells was found to be limited. even at a multiplicity of infection (moi) of 500 plaque-forming units per cell, an average of 63% cells was found to synthesize viral antigens stainable by direct immunofluorescence. when the macrophages obtained from rabbits hyperimmunized by an intravenous injection of sendai virus were infected under the same in vitro conditions, the rate of antigen synth ... | 1975 | 168154 |
| haemoglobin synthesis in fused cells. | when primitive erythroid cells from 5-day-old chick embryos are exposed to inactivated sendai virus they do not undergo haemolysis but fuse with other cells by the normal process of cytoplasmic coalescence. in this way cells actively engaged in the synthesis of haemoglobin may be fused with others that are not. in heterokaryons formed by the fusion of such erythroid cells with cells from established mouse or hamster lines, haemoglobin synthesis initially continues at a high level, but then decli ... | 1975 | 168212 |
| the action of vibrio cholerae and corynebacterium diphtheriae neuraminidases on the sendai virus receptor of human erythrocytes. | studies of the sendai virus haemagglutinin receptor on the human erythrocyte surface have confirmed that it involves 2 leads to 3 linked sialic acid. because the primary specificity of vibrio cholerae neuraminidase is for this linkage, it is able to compete with the virus for the receptor, to which it adsorbs strongly at low temperatures. corynebacterium diphtheriae neuraminidase, whose principal specificity is for a sialic acid linkage other than 2 leads to 3, does not easily remove sendai viru ... | 1975 | 168311 |
| murine virus susceptibility of cell cultures of mouse, rat, hamster, monkey, and human origin. | studies were initiated to determine the practicality of using various tissue cultures for the propagation of murine viruses isolated from laboratory animals. the cytopathogenic effects of 10 murine viruses known to cause disease in laboratory rodents were compared in monolayer cultures of l929, bhk-21, wi-38, bsc-1, and vero cells. the susceptibility of primary hamster embryo, hamster kidney, mouse embryo, mouse kidney, and rat embryo cell cultures was also tested. seven of the viruses produced ... | 1975 | 168428 |
| application of agar-gel precipitin test for the detection of sendai virus antibody in rat sera. | a simplified agar-gel precipitin test was performed for the detection of sendai virus antibody in rat sera. a close correlation was observed between detection of antibodies by complement-fixation test and agar-gel precipitin test. no correlation was found between results obtained by hemagglutination-inhibition test and agar-gel precipitin test in sera with hi titer of less than 1:8. | 1975 | 168429 |
| inhibition by interferon of delayed-type hypersensitivity in the mouse. | the effect of interferon on delayed-type by persensitivity to picryl chloride and sheep erythrocytes was examined in the mouse. when administered to sensitized animals on the day before or the day of challenge, tissue culture interferon inhibited both the ear swelling induced by pieryl chloride and footpad swelling induced by sheep erythrocytes. newcastle disease virus, when injected into sensitized if-1l or if-1h congenic mice a few hours before challenge, caused an inhibition of delayed-type h ... | 1975 | 168573 |
| somatic hybrid of thymus leukemia (. | a somatic hybrid of asl-1 leukemia cells [h-2a, thymus leukemia (tl)1,2,3, thy-1b] and lm(tk)-cells [h-2-k, tl(-), thy-1 (-), thymidine kinase deficient] was formed with the aid of inactivated sendai virus. the selection of hybrid cell clones was facilitated by the inability of asl-1 cells to grow in vitro and of lm(tk)-cells to survive in hypoxanthine/amethopterin/thymidine medium. the h-2 antigens of both parental cells were formed in approximately equivalent amounts by the hybrid cells, and t ... | 1975 | 168580 |
| lipids and cell fusion in vitro: effect of amphotericin b. | fusion rate of cl-1d cells mediated by sendai virus was decreased by preincubating the cells or the virus in the presence of lysolecithin. however, a significant increase occurred when the cells were preincubated in the presence of amphotericin b. this increase was suppressed by exogenous cholesterol. | 1975 | 168588 |
| experimental parainfluenza type 1 virus-induced encephalomyelopathy in adult mouse: a histopathological study. | the pathogenicity of a strain of parainfluenza type 1 virus, isolated from the cultured brain cells from multiple sclerosis patients and designated as 6/94 virus, was studied in mouse brain. selective degeneration of cerebral white matter, preceded by mononuclear cell infiltration, and ependymitis were prominent pathological features of the mouse intracerebrally inoculated with 6/94 virus. after inactivation by ultraviolet light the virus was still capable of producing the inflammatory and degen ... | 1975 | 168719 |
| microinjection of thymidine kinase and bovine serum albumin into mammalian cells by fusion with red blood cells. | a procedure is described by which proteins can be rapidly and efficiently microinjected into large numbers of culture cells. proteins were first introduced into mammalian red blood cells during hypotonic hemolysis, and the resealed red cells were subsequently fused to culture cells using sendai virus. in seven experiments, thymidine kinase or 125i-bsa were transferred to culture cells during fusion. although proteins were used in the present experiments, the microinjection procedure should work ... | 1975 | 168973 |
| in vitro and in vivo effect of 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide (ribavirin) on types 1 and 3 parainfulenza virus infections. | 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide (ribavirin) had significant in vitro activity against type 1 parainfluenza (sendai) and type 3 parainogenic (ha-1) viruses. activity was manifested as inhibition of both viral cytopathogenic effect and of recoverable virus or viral hemagglutinin titer. the minimum sendai virus inhibitory concentration was determined to be approximately 3.2 mug/ml. previous studies had determined the minimum concentration inhibiting ha-1 virus was approximately ... | 1975 | 169109 |
| modification of cell membranes with viral envelopes during fusion of cells with hvj (sendai virus). | | 1975 | 169133 |
| modification of cell membranes with viral envelopes during fusion cells with hvj (sendai virus). ii. effects of pretreatment with a small number of hvj. | | 1975 | 169134 |
| in vitro cellular responsiveness in multiple sclerosis patients to a viral isolate from multiple sclerosis brain tissue and to other antigens. | the clausen modification of the leukocyte migration test was used to test patients with multiple sclerosis, normal subjects, and patients with other neurologic diseases for cell-mediated immunity to 6/94 virus (a parainfluenza virus previously isolated from the brain tissue of a multiple sclerosis patient), c-rna virus isolated from a tumor, and the nonviral antigens candida and purified protein derivative. leukocytes of multiple sclerosis patient showed significantly less mean inhibition of mig ... | 1975 | 169493 |
| a temperature-sensitive mutant of sendai virus with an altered hemagglutinin-neuraminidase polypeptide: consequences for virus assembly and cytopathology. | | 1975 | 169624 |
| production of human lymphoblastoid interferon. | the interferon response of 21 lines of human lymphoblasts varied greatly. interferon from the best producer (11,000 u/ml) resembled human leukocyte interferon. | 1975 | 170301 |
| influence of oxygen and culture media on maintenance of whole hamster trachea organ cultures and replication of sendai virus. | effects of various oxygen concentrations and culture media on the maintenance of 4-day-old hamster trachea organ cultures and the yield of sendai virus were studied. the basic media used were eagle minimal essential medium, medium 199, and cmrl 1066 supplemented with glutamine and antibiotics and buffered with nahco3 or n-2-hydroxyethyl-piperazine-n'-2'-ethanesulfonic acid (hepes). in addition, each medium was evaluated under a gas phase of 5% co2 and 95% o2, 5% co2 and 45% o2 and 50% n2, or 5% ... | 1975 | 170302 |
| sulfated components of enveloped viruses. | the glycoproteins of several enveloped viruses, grown in a variety of cell types, are labeled with 35so4(-2), whereas the nonglycosylated proteins are not. this was shown for the hn and f glycoproteins of sv5 and sendai virus, the e1 and e2 glycoproteins of sindbis virus, and for the major glycoprotein, gp69, as well as for a minor glycoprotein, gp52, of rauscher leukemia virus. the minor glycoprotein of rauscher leukemia virus is more highly sulfated, with a ratio of 35so4- [3h]glucosamine abou ... | 1975 | 170420 |
| interferon assessment by the immunofluorescent, immunoperoxidase, and hemadsorption cell-counting techniques. | | 1975 | 170887 |
| decreased interferon response by lymphocytes from children with chronic hepatitis. | since a decreased interferon response has been linked with certain chronic viral infections, a preliminary study was undertaken to determine whether an altered interferon response may be involved in the pathogenesis of chronic hepatitis, a complication of type b hepatitis. the production of interferon by lymphocytes from patients with chronic hepatitis was compared with the production by lymphocytes from normal subjects. the amount of interferon produced by paramyxovirus-stimulated lymphocytes f ... | 1975 | 171316 |
| 'phagocytosis' of sendai virus by model membranes. | sendai viruses were attached to liposomes (vesicular model membranes) at 0 to 4 degrees c, and were then incubated at 37 degrees c. liposomes made of phosphatidylcholine, cholesterol and gangliosides enveloped the viruses at 37 degrees c to give a picture that resembles the ingestion step of phagocytosis. virus particles were enveloped only by liposomes that contained gangliosides which serve as sendai virus receptors. | 1975 | 171337 |
| the smallest protein of sendi virus: its candidate function of binding nucleocaspsid to envelope. | | 1975 | 171833 |
| viability of cells reconstituted by virus-induced fusion of minicells with anucleate cells. | | 1975 | 172343 |