| [seed transmission of cherry leaf roll virus in black cherry (prunus serotina ehrh.)]. | | 1976 | 947109 |
| plant virus detection using a new form of indirect elisa. | a novel form of indirect enzyme-linked immunosorbent assay (elisa) has been devised for the detection of viruses in plants. the method uses protein a in two applications to sandwich antibody-antigen-antibody layers. the first applied layer of protein a prepares the plate for the coating antibody layer. the second layer of protein a is conjugated to the enzyme and detects the second antibody layer. the orientation of the igg induced in the coating layer of antibody prevents later unwanted reactio ... | 1985 | 4055975 |
| [transmissibility of cherry leaf-roll virus by seeds of sambucus racemosa l]. | | 1972 | 4679142 |
| [cherry leaf-roll virus from birch (betula pendula roth)]. | | 1972 | 5068493 |
| sequence analysis of the 3' termini of rna1 and rna2 of blueberry leaf mottle virus. | the 3' termini of rna1 and rna2 of blueberry leaf mottle virus (bblmv) were cloned and the cdna sequence of a portion of the putative polymerase gene, the complete coat protein (cp) gene, and the 3' non-coding regions was determined. the n terminus of the coat protein gene was precisely located by comparison with the amino acid sequence determined by the edman degradation sequencing of the purified coat protein. the coat protein gene encoded a polypeptide of 521 amino acids with a predicted m(r) ... | 1994 | 7975879 |
| the identification, cloning, and sequence analysis of the coat protein coding region of a birch isolate (i2) of cherry leaf roll nepovirus. | the coat protein gene of rna-2 of cherry leaf roll nepovirus (clrv) birch isolate i2 was cloned, identified, and sequenced. transcripts derived from cdna to the coat protein gene made a polypeptide of m(r) 51.5 k when translated in vitro. the predicted amino acid sequence of the coat protein showed little identity with nepoviruses having small rna-2s. it did, however, have 27% sequence identity with the coat protein of tomato ringspot nepovirus which, like clrv, has a relatively large rna-2. | 1993 | 8328915 |
| single-step multiplex rt-pcr for simultaneous and colourimetric detection of six rna viruses in olive trees. | a single-step multiplex rt-pcr was developed for the simultaneous and colourimetric detection of six rna viruses (cucumber mosaic virus, cherry leaf roll virus, strawberry latent ringspot virus, arabis mosaic virus, olive latent-1 virus and olive latent-2 virus) which infect olive trees. six compatible primer set for one-step rt-pcr amplification in a single closed-tube and 3' digoxigenin labelled probes were designed in optimal, specific and conserved regions. the method has been assessed with ... | 2001 | 11516487 |
| walnut (juglans). | walnut species are important nut and timber producers in temperate regions of europe, asia, south america, and north america. trees can be impacted by phytophthora, crown gall, nematodes, and cherry leaf roll virus; nuts can be severely damaged by codling moth and xanthomonas blight. the long-generation time of walnuts and an absence of identified natural resistance for most of these problems suggest biotechnological approaches to crop improvement. described here is a somatic embryo based transf ... | 2006 | 17033072 |
| complete nucleotide sequence of cherry leaf roll virus (clrv), a subgroup c nepovirus. | the complete nucleotide sequence of both genomic (+)ss rnas of a rhubarb isolate of cherry leaf roll virus (clrv) was determined. the larger rna1 is 7918 nucleotides and the shorter rna2 6360 nucleotides in size, each genome component comprising a single open reading frame (orf). the rna1-encoded polyprotein (p1) is 2112 amino acids long (235.6kda) containing domains characteristic for a proteinase-cofactor (pco), nucleotide-binding helicase (hel), genome-linked protein (vpg), proteinase (pro), ... | 2011 | 22230314 |
| comparison of diagnostic techniques for the detection and differentiation of cherry leaf roll virus strains for quarantine purposes. | some strains of cherry leaf roll virus (clrv) are considered as quarantine pests in new zealand. clrv was detected in seven plant host species: actinidia chinensis, hydrangea macrophylla, malus domestica, plantago major, ribes rubrum, rubus idaeus and rumex sp. collected from new zealand between 2005 and 2012. biological, serological and molecular techniques were compared for the detection and differentiation of clrv isolates. the biological analysis revealed differences in symptomatology and di ... | 2016 | 27129669 |
| host species-dependent population structure of a pollen-borne plant virus, cherry leaf roll virus. | cherry leaf roll virus (clrv) belongs to the nepovirus genus within the family comoviridae. it has a host range which includes a number of wild tree and shrub species. the serological and molecular diversity of clrv was assessed using a collection of isolates and samples recovered from woody and herbaceous host plants from different geographical origins. molecular diversity was assessed by sequencing a short (375-bp) region of the 3' noncoding region (ncr) of the genomic rnas while serological d ... | 2006 | 16474152 |
| a multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees. | a multiplex polymerase chain reaction (mpcr) assay was developed to detect six rna and one dna citrus virus: citrus leaf rugose virus (clrv), citrus psorosis virus (cpsv), citrus tatter leaf virus (ctlv), citrus tristeza virus (ctv), citrus variegation virus (cvv), citrus yellow mosaic virus (cymv), and indian citrus ringspot virus (icrsv) from citrus plants. these seven viruses are classified in six different virus genera. degenerate primers were designed based on the respective virus isolate s ... | 2005 | 15951030 |
| simultaneous and co-operational amplification (co-pcr): a new concept for detection of plant viruses. | a new and highly sensitive method for the amplification of viral rna targets from plant material has been developed and patented. this technique called co-operational amplification (co-pcr) can be carried out easily in a simple tetraprimer reaction based on the simultaneous action of four primers. the reaction process consists of the simultaneous reverse transcription of two different fragments from the same target, one containing the other; the production of four amplicons by the combination of ... | 2002 | 12367729 |
| transient gene expression of antisense rna and coat protein-encoding sequences reduced accumulation of cherry leafroll virus in tobacco protoplasts. | cherry leafroll nepovirus (clrv) is the causative agent of blackline disease, which results in a fatal necrosis of the graft union of english walnut scion on certain rootstocks. tobacco suspension cell protoplasts were electroporated with plasmid constructions, bearing or not bearing rna-derived sequences, and, subsequently, were electroporated with clrv virions or virion rna replication of clrv in protoplasts was demonstrated by accumulation of both positive- and negative-sense clrv genomic rna ... | 1995 | 11831693 |
| subcellular localisation of cherry leaf roll virus coat protein and genomic rnas in tobacco leaves. | the in vivo subcellular location of the coat protein and rnas of cherry leaf roll nepovirus (clrv) was studied in infected tobacco plants by two different approaches and it was correlated with the cytopathic structures induced by the virus. subcellular fractions were obtained by differential centrifugation, visualised by electron microscopy and analysed for their viral rna and coat protein content by northern blot and western blot analysis, respectively. results indicate that viral rnas accumula ... | 2000 | 10717317 |
| evidence for participation of rna 1-encoded elicitor in cowpea mosaic virus-mediated concurrent protection. | the cowpea (vigna unguiculata) line arlington, inoculated with cowpea mosaic virus (cpmv), showed no symptoms, and no infectivity or accumulation of capsid antigen was detected at several days after inoculation. coinoculation, but not sequential inoculation, of cpmv with similar concentrations of another comovirus; cowpea severe mosaic virus (cpsmv), resulted in reduced numbers of cpsmv-induced lesions. this apparent, cpmv-mediated reduction in number of cpsmv-induced infection centers was terme ... | 2000 | 10639316 |
| in vitro evidence for rna binding properties of the coat protein of prunus necrotic ringspot ilarvirus and their comparison to related and unrelated viruses. | the rna binding properties of the prunus necrotic ringspot virus (pnrsv) coat protein (cp) were demonstrated by northwestern and dot-blot analyses. the capability to bind pnrsv rna 4 was compared with viruses representing three different interactions prevailing in the assembly and architecture of virions. the results showed that cucumber mosaic virus (cmv) and pnrsv cps, which stabilise their virions mainly through rna-protein interactions bound pnrsv rna 4 even at very high salt concentrations. ... | 1999 | 10365170 |
| occurrence of nepoviruses in rubus species in the czech republic. | the occurrence of arabis mosaic virus (amv), raspberry ringspot virus (rrv), tomato black ring virus (tbrv), strawberry latent ringspot virus (slrv) and cherry leaf roll virus (clrv) in cultivated and wild plants of raspberry and blackberry has been studied in the czech republic in 1993-1996. five hundred and seventy samples were collected at 51 localities and assayed by double antibody sandwich enzyme-linked immunosorbent assay (das-elisa). the results represent the first evidence on the occurr ... | 1997 | 9385408 |
| long-distance movement of cherry leaf roll virus in infected tobacco plants. | the long-distance movement of cherry leaf roll virus (clrv) in tobacco plants was studied using a tissue printing technique with non-isotopic rna probes. time-course analysis revealed that clrv rna accumulated in the inoculated leaf at an early stage, such as 20 h post-inoculation. the virus accumulation reached a peak at 8-10 days post-inoculation (d.p.i.) and then progressively decreased. the virus rna signal was detected before the appearance of symptoms. the virus invaded stem vascular tissu ... | 1996 | 8601792 |
| long, nearly identical untranslated sequences at the 3' terminal regions of the genomic rnas of cherry leafroll virus (walnut strain). | hybridization analyses of cdna clones derived from the two genomic rnas, rna1 and rna2, of the walnut strain of the nepovirus cherry leafroll nepovirus (wclrv) demonstrated a long region of high homology between the two viral rnas. subsequent mapping and nucleotide sequencing revealed a long, noncoding, presumably untranslated, region (3' utr) immediately 5' of the terminal polyadenylate, a region that is almost identical in the two rnas. this 3' utr is 1567 nucleotide residues long in rna1. hom ... | 1995 | 8560786 |
| a method combining immunocapture and pcr amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. | a method for the detection of rna viral and subviral plant pathogens was developed that combines pathogen partial purification by solid-phase adsorbed antibodies, reverse transcriptional-polymerase chain reaction (rt-pcr) and quantitation of the amplified products by fluorescence. the reverse transcription of the rna is performed directly on the retained material without any previous thermal or chemical disruption of the virus particles. the whole procedure can be carried out in a microtiter pla ... | 1993 | 8113346 |
| the multiplication in plants of arabis mosaic virus satellite rna requires the encoded protein. | oligonucleotide-directed mutagenesis was used to create two mutations at each of three positions within the open reading frame (orf) of a cdna clone representing a satellite rna from a lilac isolate of arabis mosaic nepovirus (armv). three of the six mutants, in which stop codons were introduced at three different sites, did not direct synthesis of a translation product. the other three mutants, in which stop codons were not introduced, directed synthesis of a translation product (39k) although, ... | 1993 | 7687652 |
| a subgenomic rna associated with cherry leafroll virus infections. | cherry leafroll nepovirus (clrv) genomic rna 1 (8 kb) and genomic rna 2 (7 kb) have 3' polyadenylate tracts and, extending 5' from the polyadenylate, nearly identical sequences of 1.6 kb termed the 3' common region. we observed rnas 1 and 2 and a third rna of 1.5 kb in nucleic acid extracts of clrv-infected nicotiana tabacum suspension cell protoplasts and chenopodium quinoa plants, using a hybridization probe complementary to 1 kb of the 3' common region. the third rna was partially purified by ... | 1995 | 7645232 |
| chemiluminescent and colorigenic detection of cherry leaf roll virus with digoxigenin-labeled rna probes. | digoxigenin-labeled rna probes were used to detect cherry leaf roll virus in infected plants. a dot-blot hybridization immunoenzymatic assay in both crude sap extracts and partially purified tissue with a colorigenic and chemiluminescent detection was developed. the use of the new amppd substrate was found to be effective in clarified sap extracts in conditions were the colorigenic detection method failed. both detection assays were effective when using unfractionated nucleic acid preparations, ... | 1993 | 7505786 |
| [demonstration of the relationship between the origins of cherry leaf-roll virus and elm mosaic virus]. | | 1972 | 4628106 |
| synthesis and proteolytic processing of arabis mosaic nepovirus, cherry leaf roll nepovirus, and strawberry latent ringspot nepovirus proteins in reticulocyte lysate. | the genomic rna components of three nepoviruses, arabis mosaic (armv), cherry leaf roll (clrv), and strawberry latent ringspot (slrv), were translated in rabbit reticulocyte lysate. each component (except the rna-2 of clrv) directed the synthesis of proteins that corresponded in size to their theoretical coding capacity. the rna-1 components of all three viruses were translated to yield polyproteins of mr 250k, which were autocatalytically processed to yield up to five cleavage products. the pri ... | 1991 | 1929877 |
| an appraisal of different methods for the detection of the walnut strain of cherry leafroll virus. | three methods were evaluated for the detection of cherry leafroll virus: elisa, dot-blot and reverse transcriptional polymerase chain reaction (rt-pcr). dot-blot and rt-pcr were carried out in crude plant extracts without any further rna purification. dot-blot hybridization using a 32p-labelled dna probe was as sensitive as previously reported elisa results for cherry leafroll virus detection. the most sensitive method was rt-pcr, which amplified a specific fragment of 448 bp from the 3' untrans ... | 1992 | 1551937 |
| a 1.5 kb sequence homology in 3'-terminal regions of rna-1 and rna-2 of a birch isolate of cherry leaf roll nepovirus is also present, in part, in a rhubarb isolate. | a series of cdna clones has been made from the birch isolate of cherry leaf roll nepovirus. restriction enzyme analysis and sequencing showed that at the 3' end, rna-1 and rna-2 are identical for 1.5 kb. also a 0.7 kb 3' end homology exists between the birch and rhubarb isolate. these sequences do not seem to code for any proteins; however, the sequence conservation points to a role in virus replication. | 1992 | 1538198 |
| cross-reacting and heterospecific monoclonal antibodies produced against arabis mosaic nepovirus. | monoclonal antibodies (mabs) were produced against arabis mosaic nepovirus (amv). a hybridoma screening procedure was applied which involved the testing of culture supernatants, before the hybridomas were cloned to single cell lines, for their reaction with eight nepoviruses [amv, cherry leafroll virus (clrv), grapevine fanleaf virus (gflv), peach rosette mosaic virus, raspberry ringspot virus (rrsv), tobacco ringspot virus, tomato black ring virus (tbrv) and tomato ringspot virus]. in addition ... | 1992 | 1402798 |
| comparison of complete nucleotide sequences and genome organization of six distinct cherry leaf roll virus isolates from new zealand. | the complete genomic sequences of six phenotypically distinct cherry leaf roll virus (clrv) isolates from new zealand were determined and compared. rna1 and rna2 are 7,919-7,921 and 6,361-6,363 nucleotides in length, respectively, excluding the poly(a) tails. both genome segments contain a single open reading frame and encode one polyprotein of 2,113 amino acids for rna1 and 1,590 aa for rna2, with corresponding molecular masses of 235.5-236.1 kda and 174.2-174.8 kda, respectively. the six rna1 ... | 2014 | 25091745 |
| walnut (juglans). | walnut species are important nut and timber producers in temperate regions of europe, asia, south america, and north america. trees can be impacted by phytophthora, crown gall, nematodes, armillaria, and cherry leaf roll virus; nuts can be severely damaged by codling moth, husk fly, and xanthomonas blight. the long generation time of walnuts and an absence of identified natural resistance for most of these problems suggest biotechnological approaches to crop improvement. described here is a soma ... | 2015 | 25416262 |
| a new virus discovered by immunocapture of double-stranded rna, a rapid method for virus enrichment in metagenomic studies. | next-generation sequencing technologies enable the rapid identification of viral infection of diseased organisms. however, despite a consistent decrease in sequencing costs, it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. as the majority of plant viruses have an rna genome, a common approach is to extract the double-stranded rna (dsrna) replicative form, to enrich the replicating virus genetic material over the host background. the tradi ... | 2016 | 26990372 |
| differentiation of cherry leaf roll virus isolates from various host plants by immunocapture-reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations. | a restriction fragment length polymorphism assay (rflp) was developed to differentiate cherry leaf roll virus (clrv) isolates according to phylogenetic clades by examining restriction patterns from partial 3' non-coding region (ncr) genomic fragments (approx. 420bp). the 3' ncr fragment from 43 clrv isolates belonging to different phylogenetic groups were compared after restriction analysis with the endonucleases bsp143i, alui, rsai, ecori and eco130i, and another 23 isolates were analyzed by co ... | 2009 | 19136029 |
| multiplex nested reverse transcription-polymerase chain reaction in a single tube for sensitive and simultaneous detection of four rna viruses and pseudomonas savastanoi pv. savastanoi in olive trees. | abstract a multiplex nested reverse transcription-polymerase chain reaction (rt-pcr) in a single closed tube was developed for the simultaneous detection of four rna viruses: cucumber mosaic virus, cherry leaf roll virus, strawberry latent ringspot virus, and arabis mosaic virus, and the bacterium pseudomonas savastanoi pv. savastanoi. the method enabled, for the first time, the sensitive and simultaneous detection of rna and dna targets from plant viruses and a bacterium, saving time, decreasin ... | 2003 | 18944338 |
| localization of transmissible and nontransmissible viruses in the vector nematode xiphinema americanum. | abstract the inner lining of the food canal of nematodes that transmit plantinfecting viruses is regarded as the retention region of viruses. to characterize the location of transmissible and nontransmissible viruses in the vector nematode xiphinema americanum, three nepoviruses, tobacco ringspot virus (trsv), tomato ringspot virus(tomrsv), and cherry leaf roll virus(clrv), and one non-nematode-transmissible virus, squash mosaic virus (sqmv), were evaluated for transmission efficiency and locali ... | 2002 | 18944262 |
| complete nucleotide sequences and genome organization of a cherry isolate of cherry leaf roll virus. | the complete nucleotide sequence of cherry leaf roll virus (clrv, genus nepovirus) from a naturally infected cherry tree (prunus avium cv. bing) in north america was determined. rna1 and rna2 consist of 7,893 and 6,492 nucleotides, respectively, plus a poly-(a) tail. each rna encodes a single potential open reading frame. the first 657 nucleotides of rna1 and rna2 are 99% identical and include the 5'-utr and the first 214 deduced amino acids of the polyproteins following the first of two in-fram ... | 2012 | 22218961 |