| purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in australia. | isometric virus-like particles, 18 nm in diameter, have been isolated from banana (musa spp.) affected by bunchy top disease in australia. banana bunchy top disease-associated virus-like particles (bbtv) banded as a single component with buoyant density of 1.28 to 1.29 g/ml in cs2so4 and sedimented at about 46s in isokinetic sucrose density gradients. the a260/a280 of purified preparations was about 1.33. a single coat protein of mr 20,500 was identified with antibodies to bbtv particles from au ... | 1991 | 1993863 |
| virus-like particles associated with banana bunchy top disease contain small single-stranded dna. | virus-like particles were purified from banana plants with banana bunchy top disease. these particles were isometric with a diameter of 18 to 20 nm and a density of 1.28 to 1.30 g/ml in caesium sulphate. associated with these particles were an ssdna of about 1 kb and one major protein of mr 20,100. dsdna was synthesized from nucleic acid extracts from these particles and cloned. one clone, pbt338, hybridized specifically (i) with sap extracts from plants infected with banana bunchy top virus (bb ... | 1991 | 1993864 |
| movement and transmission of banana bunchy top virus dna component one in bananas. | the systemic movement and replication of banana bunchy top virus (bbtv) dna component one were investigated. strand-specific rna probes and pcr were used to indicate the presence of the virus in various parts of infected banana plants during infection on the basis of dsdna replicative intermediates of bbtv. the strand-specific probes were not only able to detect the presence of the virus but also gave an indication of where the virus replicated. the results using both the virion sense and comple ... | 1995 | 7561765 |
| the genome organization of banana bunchy top virus: analysis of six ssdna components. | we have cloned, sequenced and analysed an additional five circular ssdna components of banana bunchy top virus (bbtv) which we have called components 2, 3, 4, 5 and 6. these components were present in all bbtv infections tested. four of these components (components 3, 4, 5 and 6) had one large open reading frame (orf) in the virion sense located 3' of a stem-loop structure. each orf had a potential tata box and one or two potential polyadenylation signals associated with it and each polyadenylat ... | 1995 | 7782775 |
| sequence of subterranean clover stunt virus dna: affinities with the geminiviruses. | the nucleotide sequences of seven circular, single-stranded dna components of one isolate of subterranean clover stunt virus (scsv) have been determined. each component, of about 1 kb, appears to encode a single open reading frame in the same sense as the encapsidated dna. notably, the proteins encoded by two scsv components are related. each has a consensus nucleotide binding motif and shares about 40% amino acid identity with the other and with the putative replication proteins of banana bunch ... | 1995 | 7886939 |
| evidence that banana bunchy top virus has a multiple component genome. | a 93 nucleotide sequence was found to be strongly conserved between two ssdna genomic components of banana bunchy top virus (bbtv). two outwardly extending degenerate primers were designed from this sequence and used in a polymerase chain reaction (pcr) with dna extracted from purified bbtv virions. pcr amplified products consisting of at least seven distinct bands all approximately 1 kb and possibly representing full-length bbtv dsdna were resolved. the pcr amplified products were cloned and th ... | 1994 | 7944956 |
| evidence for two groups of banana bunchy top virus isolates. | banana bunchy top virus (bbtv) dna component 1 from isolates from 10 different countries was cloned and sequenced and the sequences were aligned and compared. this analysis indicated two groups: the south pacific group (isolates from australia, burundi, egypt, fiji, india, tonga and western samoa) and the asian group (isolates from the philippines, taiwan and vietnam). the mean sequence difference within each group was 1.9 to 3.0% and between isolates from the two groups was approximately 10%, b ... | 1994 | 7996145 |
| genome characterization and identification of viral-associated dsdna component of banana bunchy top virus. | the single-stranded (ss) and double-stranded (ds) viral-associated dnas (vads) of banana bunchy top virus (bbtv) were characterized. the ssvads were shown to be the viral genomic dna and the dsvads are the likely replication forms. both dsvads and ssvads contain dna species that cross-hybridize to each other. under the electron microscope, both circular and linear dnas were observed from these vad species. libraries were constructed from vads to further characterize them. two overlapping vad clo ... | 1994 | 8291246 |
| nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. | one dna component of the banana bunchy top virus (bbtv) genome was cloned and sequenced. this component is present as a circular, ssdna in the virions and consists of 1111 nucleotides. it contains one large open reading frame (orf) of 858 nucleotides in the virion sense; this orf encodes a putative replicase based on the presence of a dntp-binding motif (ggegkt). two smaller orfs (249 and 366 nucleotides), in the complementary orientation, could not be assigned any obvious function. neither of t ... | 1993 | 8445361 |
| putative full-length clones of the genomic dna segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein. | subterranean clover stunt disease is an economically important aphid-borne virus disease affecting certain pasture and grain legumes in australia. the virus associated with the disease, subterranean clover stunt virus (scsv), was previously found to be representative of a new type of single-stranded dna virus. analysis of the virion dna and restriction mapping of double-stranded cdna synthesized from virion dna suggested that scsv has a segmented genome composed of 3 or 4 different species of ci ... | 1993 | 8460528 |
| detection of dna and rna plant viruses by pcr and rt-pcr using a rapid virus release protocol without tissue homogenization. | a simple, single-step plant tissue preparation protocol suitable for the detection of viruses by the polymerase chain reaction and reverse transcription-polymerase chain reaction is described. the effect of buffer components and ph, and the incubation temperature for the release of virus from plant material was evaluated. a small amount of plant tissue was heated in a solution containing 100 mm tris-hcl, ph 7.4 or 8.4, 1 m kcl and 10 mm edta for 10 min at 95 degrees c and the supernatant used fo ... | 1995 | 8530569 |
| sequence of porcine circovirus dna: affinities with plant circoviruses. | the complete nucleotide sequence (1759 nt) of the ssdna genome of porcine circovirus (pcv) was determined from a cloned dsdna replicative form isolated from pcv-infected cells. sequence analysis detected no significant nucleic acid or protein similarity with another animal circovirus, chicken anaemia virus (cav) but, surprisingly, the highest protein similarity was obtained between the product of the largest predicted pcv orf (orf1; encoding a potential protein of 35.7 kda) and a putative protei ... | 1997 | 9010307 |
| two mrnas are transcribed from banana bunchy top virus dna-1. | we have mapped the mrna transcripts of banana bunchy top virus (bbtv) dna-1. northern hybridization and 3' race analysis identified two poly-adenylated rnas associated with bbtv dna-1. previously, one major orf in the virion sense of dna-1 had been identified, which encoded a putative replication protein (rep). an mrna was identified in bbtv infected bananas that was clearly transcribed from this rep orf. further, a second transcript was identified which mapped to an orf completely within the re ... | 1997 | 9010308 |
| a dna primer associated with banana bunchy top virus. | banana bunchy top virus (bbtv) genomic ssdna is capable of complementary strand synthesis in vitro without the addition of exogenous primers. we have demonstrated that the self-priming of bbtv can be attributed to a population of endogenous primers which are bound to the genomic dna within the virions. the primer molecules appeared to be composed entirely of dna and are heterogeneous in size. the primers were cloned, sequenced and shown to map to a region within the major common region and exten ... | 1997 | 9018072 |
| analysis of six dna components of the faba bean necrotic yellows virus genome and their structural affinity to related plant virus genomes. | faba bean necrotic yellows virus (fbnyv) has a multicomponent circular ssdna genome. in addition to a previously described genome component (c1) coding for a replicase-associated protein (rep), five further components (c2 to c6) have now been identified. each of the six components is about 1 kb in size, contains one major open reading frame (orf) in the virion sense with a tata box and polyadenylation signal, and has a noncoding region containing a highly conserved sequence possibly forming a st ... | 1997 | 9217049 |
| nicking and joining activity of banana bunchy top virus replication protein in vitro. | the major open reading frame of banana bunchy top virus (bbtv) dna-1 encodes a putative replication initiation protein (rep). in vitro, a fusion protein of bbtv rep linked to a maltose-binding protein exhibited both site-specific nicking and joining activities. these activities were dependent on the presence of mg2+ or mn2+, but did not require atp. the fusion protein specifically cleaved ssdna between bases +7 and +8 of a conserved nonanucleotide loop sequence which is present in the virion-str ... | 1997 | 9225058 |
| banana bunchy top virus dna-3 encodes the viral coat protein. | banana bunchy top virus (bbtv) has a multicomponent genome consisting of at least six circular single-stranded dnas each with a single large open reading frame (orf) in the virion sense. a protein of approximately 20 kda has been associated with purified virions and is assumed to be the viral coat protein. the n-terminus of this protein was sequenced and compared to the predicted amino acid sequence of the large orf of bbtv dna-1 to 6. this comparison indicated that the orf of bbtv dna-3, which ... | 1997 | 9672627 |
| promoter activity associated with the intergenic regions of banana bunchy top virus dna-1 to -6 in transgenic tobacco and banana cells. | promoter regions associated with each of the six ssdna components of banana bunchy top virus (bbtv) have been characterized. dna segments incorporating the intergenic regions of bbtv dna-1 to -6 were isolated and fused to the uida (beta-glucuronidase) reporter gene to assess promoter activity. in tobacco cell suspensions, the bbtv dna-2 and -6 promoters generated levels of gus expression 2-fold greater and similar to the 800 bp camv 35s promoter, respectively. deletion analysis of the bbtv dna-6 ... | 1998 | 9780033 |
| beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. | circoviruses are a diverse group of animal and plant pathogens with undefined relationships to one another but for their non-geminate, non-enveloped capsids and circular, single-stranded dna genomes. the sequences of the beak and feather disease virus and porcine circovirus genomic dnas are presented and analyzed in the context of the other members of the family. sequence comparisons, inferred phylogenies, and geographic occurrence suggest that the ambisense circoviruses, particularly the beak a ... | 1998 | 9787657 |
| ten distinct circular ssdna components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome. | four further circular ssdna components (c7-c10), about 1 kb in size and structurally similar to the previously described components (c1-c6) found associated with a syrian (sy) isolate of faba bean necrotic yellows virus (fbnyv), have been identified. similar to c1 and c2, two of the new components (c7 and c9) encode putative replication-associated (rep) proteins of 33.2 and 32.7 kda, respectively, the former of which is 90% identical to the c10 rep protein of milk vetch dwarf virus (mdv). c8 enc ... | 1998 | 9880028 |
| banana bunchy top virus dna-2 to 6 are monocistronic. | banana bunchy top virus (bbtv) dna-3 to 6 have each previously been shown to contain one large open reading frame in the virion sense, whereas no large orf had been identified in bbtv dna-2. rnas transcribed from the bbtv genome were mapped using northern hybridisation and 3' race. one mrna was transcribed from each of bbtv dna-2 to 6 and four of these mrnas mapped to the orfs previously identified in bbtv dna-3 to 6. the mrna of bbtv dna-2 was transcribed from a virion sense orf probably using ... | 1999 | 10076511 |
| functional analysis of proteins encoded by banana bunchy top virus dna-4 to -6. | green fluorescent protein (gfp)-tagging was used to determine the intracellular localization pattern of the proteins encoded by banana bunchy top virus (bbtv) dna-3, -4 and -6. the protein encoded by bbtv dna-4, which possesses a hydrophobic n terminus, was found to localize exclusively to the cell periphery while the proteins encoded by bbtv dna-3 and -6 were found in both the nucleus and the cytoplasm. co-expression of the dna-4 protein and the proteins encoded by bbtv dna-3 and -6 revealed th ... | 2000 | 10640570 |
| sequence variability in the coat protein gene of two groups of banana bunchy top isolates. | complete nucleotide sequences of the coat protein gene (dna-3) of banana bunchy top virus (bbtv) were obtained from five geographical isolates by pcr. analysis of these sequences revealed two distinct groups of bbtv isolates with those from the philippines, taiwan and vietnam forming the asian group while the south pacific/african group consisted of isolates from australia, burundi and fiji. at the nucleotide level, the sequences of dna-3 were more similar between isolates from the same group (m ... | 2000 | 10795525 |
| development of a multiplex immunocapture pcr with colourimetric detection for viruses of banana. | a multiplex, immunocapture pcr (m-ic-pcr) was developed for the simultaneous detection of three viruses from crude sap extracts of banana and plantain (musa spp.). a reverse transcription step was required for banana bract mosaic virus and cucumber mosaic virus, which have ssrna genomes. the detection of banana bunchy top virus (ssdna genome) was not adversely affected by inclusion in this step. all the three viruses could be detected simultaneously from a mixed infection. identification and det ... | 2000 | 10996641 |
| additional rep-encoding dnas associated with banana bunchy top virus. | banana bunchy top nanovirus (bbtv) has a multicomponent circular single-stranded dna (cssdna) genome consisting of at least six components. we have cloned, sequenced and analysed two additional cssdna components, designated bbtv-s1 and s2, associated with a taiwanese bbtv isolate. the sequences of bbtv-s1 and s2 comprised 1109 and 1095 nucleotides (nt), respectively, and like bbtv dna-1, potentially encoded replication initiation proteins (reps). however, the genome organisation of bbtv-s1 and s ... | 2001 | 11266219 |
| [specific amplification of the coding sequences of bbtv iii, iv, i and their application in bbtv detection]. | banana bunchy top virus disease (bbtd) is a disastrous disease in bananas, and it is spreading in the world (including china) by the banana bunchy top virus(bbtv). at present, virus-free plantlets are used to prevent bbtd in banana production, therefore, it is very important to establish a method to detect bbtv quickly, sensitively and specifically. elisa is now popularly used to detect bbtv. the sensitivity of this method is not high enough, and needs specific antiserum, otherwise, pseudo-posit ... | 2001 | 12549199 |
| birth-and-death evolution of protein-coding regions and concerted evolution of non-coding regions in the multi-component genomes of nanoviruses. | genomes of the four plant viruses of the genus nanovirus consist of multiple circular single-stranded dna components, each of which encodes a single protein. protein phylogenies supported the hypothesis that faba bean necrotic yellows virus (fbnyv) and milk vetch disease virus (mdv) are sister taxa; that subterranean clover stunt virus (scsv) branched next; and that banana bunchy top virus (bbtv) is an outgroup to the three other species. the phylogeny of replication (rep) proteins indicate that ... | 2004 | 14715221 |
| an iterated sequence in the genome of banana bunchy top virus is essential for efficient replication. | banana bunchy top virus (bbtv) has a multi-component genome of circular, single-stranded dna. bbtv replicates via a rolling-circle mechanism, probably involving sequence-specific interaction of the replication initiation protein (rep) with iterated sequences (iterons) within the viral genome. three putative iterons (designated f1, f2 and r), with the sequence gggac, have been identified in the intergenic region of each bbtv component. to investigate their role in replication, each of the iterons ... | 2006 | 17030877 |
| reassortment and concerted evolution in banana bunchy top virus genomes. | the nanovirus banana bunchy top virus (bbtv) has six standard components in its genome and occasionally contains components encoding additional rep (replication initiation protein) genes. phylogenetic network analysis of coding sequences of dna 1 and 3 confirmed the two major groups of bbtv, a pacific and an asian group, but show evidence of web-like phylogenies for some genes. phylogenetic analysis of 102 major common regions (cr-ms) from all six components showed a possible concerted evolution ... | 2007 | 17135318 |
| within-plant distribution and binomial sampling of pentalonia nigronervosa (hemiptera: aphididae) on banana. | the banana aphid, pentalonia nigronervosa coquerel (hemiptera: aphididae), infests banana (musa spp.) worldwide. pentalonia nigronervosa is the vector of banana bunchy top virus (family nanoviridae, genus babuvirus) the etiological agent of banana bunchy top disease (bbtd). bbtd is currently the most serious problem affecting banana in hawaii. despite the importance of this vector species, little is known about its biology or ecology. there are also no sampling plans available for p. nigronervos ... | 2006 | 17195692 |
| a pcr-based method, with internal control, for the detection of banana bunchy top virus in banana. | banana bunchy top disease is a major constraint to banana production in most regions where this crop is grown. the disease is caused by banana bunchy top virus (bbtv), a multicomponent, single-stranded dna virus of the family nanoviridae. we have designed primers to a conserved region of the master replication-associated protein that are useful for the polymerase chain reaction (pcr)-mediated detection of bbtv. in addition, primers to banana genomic sequence are used as an internal control, over ... | 2005 | 15920288 |
| abacá bunchy top virus, a new member of the genus babuvirus (family nanoviridae). | two isolates of a novel babuvirus causing "bunchy top" symptoms were characterised, one from abacá (musa textilis) from the philippines and one from banana (musa sp.) from sarawak (malaysia). the name abacá bunchy top virus (abtv) is proposed. both isolates have a genome of six circular dna components, each ca. 1.0-1.1 kb, analogous to those of isolates of banana bunchy top virus (bbtv). however, unlike bbtv, both abtv isolates lack an internal orf in dna-r, and the orf in dna-u3 found in some b ... | 2008 | 17978886 |
| molecular characterisation of banana bunchy top virus (bbtv) from pakistan. | banana bunchy top disease is caused by a single-stranded circular dna virus, banana bunchy top virus (bbtv), which is a member of the genus babuvirus (family nanoviridae). we have cloned and sequenced five components (dna-r, dna-s, dna-n, dna-m and dna-c) of a bbtv isolate originating from pakistan. in addition, the dna-r and several other components of five further isolates, originating from geographically distinct sites across the banana-growing area of sindh province, pakistan, were cloned an ... | 2008 | 17990091 |
| effect of temperature, vector life stage, and plant access period on transmission of banana bunchy top virus to banana. | the study of the transmission biology of insect-borne plant viruses is important to develop disease control practices. we characterized the transmission of a nanovirus, banana bunchy top virus (bbtv), by its aphid vector pentalonia nigronervosa coquerel (hemiptera, aphididae) with respect to temperature, vector life stage, and plant access time. adult aphids transmitted bbtv more efficiently than third instar nymphs at all temperatures tested. adult aphids transmitted the virus more efficiently ... | 2008 | 18944300 |
| spread of an introduced vector-borne banana virus in hawaii. | emerging diseases are increasing in incidence; therefore, understanding how pathogens are introduced into new regions and cause epidemics is of importance for the development of strategies that may hinder their spread. we used molecular data to study how a vector-borne banana virus, banana bunchy top virus (bbtv), spread in hawaii after it was first detected in 1989. our analyses suggest that bbtv was introduced once into hawaii, on the island of oahu. all other islands were infected with isolat ... | 2009 | 19037897 |
| an improved electrochemiluminescence polymerase chain reaction method for highly sensitive detection of plant viruses. | recently, we have reported an electrochemiluminescence polymerase chain reaction (ecl-pcr) method for detection of genetically modified organisms. the ecl-pcr method was further improved in the current study by introducing a multi-purpose nucleic acid sequence that was specific to the tris(bipyridine) ruthenium (tbr) labeled probe, into the 5' terminal of the primers. the method was applied to detect plant viruses. conserved sequence of the plant viruses was amplified by pcr. the product was hyb ... | 2007 | 17386503 |
| aphid transmission of banana bunchy top virus to bananas after treatment with a bananacide. | field and laboratory studies were conducted to determine the impact of using a herbicide as a bananacide on aphid transmission of banana bunchy top virus (family nanoviridae, genus babuvirus, bbtv) to healthy banana (musa spp.) plants. bbtv-infected banana plants in a commercial orchard were treated with roundup weathermax herbicide. using polymerase chain reaction, the time after herbicide treatment that bbtv could no longer be detected in the infected plants was determined. the impact of the h ... | 2009 | 19449627 |
| unusual events involved in banana bunchy top virus strain evolution. | banana bunchy top virus (bbtv) can be transmitted by aphids and consists of at least six integral components (dna-r, -u3, -s, -m, -c, and -n). several additional replication-competent components (additional reps) are associated with some bbtv isolates. a collected bbtv strain (tw3) that causes mild symptoms was selected to study the processes in bbtv evolution. southern blot hybridization, polymerase chain reaction (pcr), and real-time pcr did not detect dna-n in tw3. real-time pcr quantificatio ... | 2009 | 19522579 |
| comparative susceptibility of two banana cultivars to banana bunchy top virus under laboratory and field environments. | field and laboratory experiments were carried out on the island of oahu, hi, to compare the susceptibility of the two most commonly grown banana (musa sp.) cultivars in the state ('dwarf brazilian' or santa catarina [locally known as dwarf apple] and 'williams') to the aphid-borne banana bunchy top virus (genus babuvirus, family nanoviridae, bbtv). several morphological and physiological features of the two cultivars were monitored to determine whether the banana aphid, pentalonia nigronervosa c ... | 2009 | 19610400 |
| identification of two rna silencing suppressors from banana bunchy top virus. | in order to suppress rna silencing, many plant and some animal viruses encode rna silencing suppressors to achieve infection. in this study, we report that b3 and b4, encoded by dna3 and dna4 of banana bunchy top virus (bbtv), exhibit rna silencing suppression activity. b3 and b4 were able to increase the transient expression of green fluorescent protein (gfp) and dramatically enhanced the pathogenicity of potato virus x (pvx) in nicotiana benthamiana. b4 was able to reverse established gene sil ... | 2009 | 19816653 |
| identification of a major pathogenicity determinant and suppressors of rna silencing encoded by a south pacific isolate of banana bunchy top virus originating from pakistan. | five genes encoded by banana bunchy top virus (bbtv) originating from pakistan were expressed in nicotiana benthamiana using a potato virus x (pvx) vector. expression of the master replication-associated protein (mrep) and movement protein (mp) resulted in necrotic cell death of inoculated tissues, as well as leaf curling and necrosis along the veins in newly emerging leaves. the systemic necrosis induced by the expression of mp was discolored (dark) in comparison to that induced by mrep. expres ... | 2010 | 21161359 |
| biology of pentalonia nigronervosa (hemiptera, aphididae) on banana using different rearing methods. | the banana aphid, pentalonia nigronervosa coquerel, is present worldwide where banana (musa spp.) is grown. it is the vector of banana bunchy top virus (nanoviridae, babuvirus), the etiological agent of banana bunchy top disease, currently the most important constraint for banana producers in hawaii. p. nigronervosa is not well studied, and effects of temperature on its growth and reproduction are unknown. we studied the longevity and fecundity of one clone of banana aphid on different types of ... | 2007 | 17349115 |
| evidence of recombination in the banana bunchy top virus genome. | viruses serve as good model for evolutionary studies, owing to their short generation times and small genomes. banana bunchy top virus (bbtv) is a significant subject being multicomponent circular single stranded dna virus. bbtv belongs to family nanoviridae and contains dna-r, -u3, -s, -m, -c, and -n as integral genomic components. evolutionary studies have shown genetic re-assortment of components among its isolates and revealed a concerted type evolution in non-coding regions of its genome. t ... | 2011 | 21539936 |
| banana bunchy top virus in sub-saharan africa: investigations on virus distribution and diversity. | banana bunchy top virus (bbtv) was first reported from sub-saharan africa (ssa) from democratic republic of congo (drc) in the 1950s, has become invasive and spread into 11 countries in the region. to determine the potential threat of bbtv to the production of bananas and plantains (musa spp.) in the sub-region, field surveys were conducted for the presence of banana bunchy top disease (bbtd) in the drc, angola, cameroon, gabon and malawi. using the dna-s and dna-r segments of the virus genome, ... | 2011 | 21549775 |
| population distribution and density of pentalonia nigronervosa (hemiptera: aphididae) within banana mats: influence of plant age and height on sampling and management. | the banana aphid, pentalonia nigronervosa coquerel (hemiptera: aphididae), is the most economically important pest of banana (musa spp.) fields in hawaii. recently, there has been a concerted effort in hawaii to learn more about the biology and ecology of this pest. however, limited work has been directed at determining the distribution of p. nigronervosa in banana fields and developing an integrated pest management plan. therefore, a survey was conducted in banana fields throughout the hawaiian ... | 2011 | 21735915 |
| bioinformatic analysis of bbtv satellite dna in hainan. | banana bunchy top virus (bbtv), family nanaviridae, genus babuvirus, is a single stranded dna virus (ssdna) that causes banana bunchy top disease (bbtd) in banana plants. it is the most common and most destructive of all viruses in these plants and is widespread throughout the asia-pacific region. in this study we isolated, cloned and sequenced a bbtv sample from hainan island, china. the results from sequencing and bioinformatics analysis indicate this isolate represents a satellite dna compone ... | 2011 | 21847760 |
| mapping the 5' ends of banana bunchy top virus gene transcripts. | banana bunchy top virus (bbtv), a multi-component circular ssdna virus, replicates via a dsdna intermediate that also serves as a template for virion sense transcription. seven virus-derived transcripts have been previously identified and analysed in bbtv-infected bananas by northern analysis and 3' rapid amplification of cdna ends (3' race). in this study, we have used rna ligase-mediated rapid amplification of 5' cdna ends (rlm-race) to complete the mapping of the bbtv gene transcripts and hav ... | 2007 | 17187296 |
| localization, concentration, and transmission efficiency of banana bunchy top virus in four asexual lineages of pentalonia aphids. | banana bunchy top virus (bbtv) is the most destructive pathogenic virus of banana plants worldwide. the virus is transmitted in a circulative non-propagative manner by the banana aphid, pentalonia nigronervosa coquerel. in this work, we examined the localization, accumulation, and transmission efficiency of bbtv in four laboratory-established lineages of pentalonia aphids derived from four different host plants: taro (colocasia esculenta), heliconia (heliconia spp.), red ginger (alpinia purpurat ... | 2013 | 23435241 |
| a metagenomic approach from aphid's hemolymph sheds light on the potential roles of co-existing endosymbionts. | aphids are known to live in symbiosis with specific bacteria, called endosymbionts which can be classified as obligate or accessory. buchnera aphidicola is generally the only obligatory symbiont present in aphids, supplying essential nutrients that are missing in the plants phloem to its host. pentalonia nigronervosa is the main vector of the banana bunchy top virus, one of the most damageable viruses in banana. this aphid is carrying two symbionts: b. aphidicola (bpn) and wolbachia sp. (wpn). t ... | 2015 | 26667400 |
| a transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters. | the first generation transgenic crops used strong constitutive promoters for transgene expression. however, tissue-specific expression is desirable for more precise targeting of transgenes. moreover, piercing/sucking insects, which are generally resistant to insecticidal bacillus thuringiensis (bt) proteins, have emerged as a major pests since the introduction of transgenic crops expressing these toxins. phloem-specific promoters isolated from banana bunchy top virus (bbtv) were used for the exp ... | 2016 | 27708374 |
| biology, etiology, and control of virus diseases of banana and plantain. | banana and plantain (musa spp.), produced in 10.3 million ha in the tropics, are among the world's top 10 food crops. they are vegetatively propagated using suckers or tissue culture plants and grown almost as perennial plantations. these are prone to the accumulation of pests and pathogens, especially viruses which contribute to yield reduction and are also barriers to the international exchange of germplasm. the most economically important viruses of banana and plantain are banana bunchy top v ... | 2015 | 25591881 |
| nine novel dna components associated with the foorkey disease of large cardamom: evidence of a distinct babuvirus species in nanoviridae. | foorkey disease is a serious constraint to the production of large cardamom (amomum subulatum, family zingiberaceae). the disease is characterized by profuse proliferation of excessive stunted shoots, which makes the clump totally unproductive. the disease has been known in india since 1936 but the complete genome of the virus had not yet been characterized. in a preliminary study, an associated virus tentatively named as cardamom bushy dwarf virus (cbdv) was identified based on the partial sequ ... | 2013 | 24091364 |
| local phytochemical response of musa acuminata × balbisiana colla cv. 'bluggoe' (abb) to colonization by sternorrhyncha. | the interaction of two sternorrhyncha species, the banana aphid (pentalonia nigronervosa coquerel (hemiptera: aphididae, aphidinae)), vector of the banana bunchy top virus (bbtv), and the latania scale (hemiberlesia lataniae signoret (hemiptera: diaspididae, diaspidinae)) with musa acuminata × balbisiana colla (abb group) 'bluggoe' (musaceae) was investigated by a combination of conventional and spatially resolved analytical techniques, (1)h nmr, uhplc-ms, and matrix-free uv-laser desorption/ion ... | 2017 | 27839785 |
| low genetic diversity of banana bunchy top virus, with a sub-regional pattern of variation, in democratic republic of congo. | banana bunchy top virus (bbtv), belonging to the genus babuvirus, is the most devastating and widespread banana virus. banana and plantain are major crops in terms of household income and food security in democratic republic of congo (drc). despite the large area under banana and plantain cultivation in the country, before this study, the genetic characterization of bbtv isolates had only been undertaken for two provinces. in the study presented here, genetic variation in bbtv was assessed from ... | 2016 | 27550369 |
| the global distribution of banana bunchy top virus reveals little evidence for frequent recent, human-mediated long distance dispersal events. | banana bunchy top virus (bbtv; family nanoviridae, genus babuvirus) is a multi-component single-stranded dna virus, which infects banana plants in many regions of the world, often resulting in large-scale crop losses. we analyzed 171 banana leaf samples from fourteen countries and recovered, cloned, and sequenced 855 complete bbtv components including ninety-four full genomes. importantly, full genomes were determined from eight countries, where previously no full genomes were available (samoa, ... | 2015 | 27774281 |
| molecular characterization of banana bunchy top virus isolate from sri lanka and its genetic relationship with other isolates. | bunchy top disease of banana caused by banana bunchy top virus (bbtv, genus babuvirus family nanoviridae) is one of the most important constraints in production of banana in the different parts of the world. six genomic dna components of bbtv isolate from kandy, sri lanka (bbtv-k) were amplified by polymerase chain reaction (pcr) with specific primers using total dna extracted from banana tissues showing typical symptoms of bunchy top disease. the amplicons were of expected size of 1.0-1.1 kb, w ... | 2016 | 27366766 |
| identification and in silico characterisation of defective molecules associated with isolates of banana bunchy top virus. | banana bunchy top virus (bbtv) is a multi-component single-stranded dna virus. from 267 potentially infected musa plants, 24 apparently 'defective' bbtv components have been identified. interestingly, 23/24 of these defective molecules were apparently derived from dna-r. all of the identified defective molecules had retained at least part of the cr-sl and cr-m but had insertions and/or deletions that in most cases resulted in open reading frame disruptions. our detection of three monophyletic bu ... | 2016 | 26733296 |
| the antagonistic effect of banana bunchy top virus multifunctional protein b4 against fusarium oxysporum. | the viral-induced banana bunchy top disease and the fungal-induced banana blight are two major causes of concern for industrial scale production of bananas. banana blight is particularly troublesome, affecting ∼80% of crops worldwide. strict guidelines and protocols are in place in order to ameliorate the effects of this devastating disease, yet little success has been achieved. from the data presented here, we have found that banana bunchy top virus (bbtv)-infected bananas are more resistant to ... | 2016 | 26369403 |
| impact of diseases on export and smallholder production of banana. | banana (musa spp.) is one of the world's most valuable primary agricultural commodities. exported fruit are key commodities in several producing countries yet make up less than 15% of the total annual output of 145 million metric tons (mmt). transnational exporters market fruit of the cavendish cultivars, which are usually produced in large plantations with fixed infrastructures and high inputs of fertilizers, pesticides, and irrigation. in contrast, smallholders grow diverse cultivars, often fo ... | 2015 | 26002290 |
| localization of banana bunchy top virus and cellular compartments in gut and salivary gland tissues of the aphid vector pentalonia nigronervosa. | banana bunchy top virus (bbtv) (nanoviridae: babuvirus) is transmitted by aphids of the genus pentalonia in a circulative manner. the cellular mechanisms by which bbtv translocates from the anterior midgut to the salivary gland epithelial tissues are not understood. here, we used multiple fluorescent markers to study the distribution and the cellular localization of early and late endosomes, macropinosomes, lysosomes, microtubules, actin filaments, and lipid raft subdomains in the gut and princi ... | 2016 | 25728903 |
| miniaturized paper-based gene sensor for rapid and sensitive identification of contagious plant virus. | plant viruses cause significant production and economic losses in the agricultural industry worldwide. rapid and early identification of contagious plant viruses is an essential prerequisite for the effective control of further spreading of infection. in this work, we describe a miniaturized paper-based gene sensor for the rapid and sensitive identification of a contagious plant virus. our approach makes use of hybridization-mediated target capture based on a miniaturized lateral flow platform a ... | 2014 | 25412341 |
| identification and characterization of a distinct banana bunchy top virus isolate of pacific-indian oceans group from north-east india. | banana bunch top virus (bbtv) is considered to be a serious threat to banana production. a new isolate of the virus (bbtv-umiam) was identified and characterized from local banana mats growing in mid-hills of meghalaya in north-east india. the complete nucleotide sequence analysis revealed the presence of six full-length ssdna components (dna r, dna u3, dna s, dna m, dna c and dna n) sharing major common region (cr-m) and a stem-loop common region (cr-sl). bbtv-umiam showed a unique deletion of ... | 2014 | 24468493 |
| natural occurrence of banana bunchy top virus in ensete superbum in india. | | 2013 | 24426269 |
| application of motif-based tools on evolutionary analysis of multipartite single-stranded dna viruses. | multipartite viruses contain more than one distinctive genome component, and the origin of multipartite viruses has been suggested to evolve from a non-segmented wild-type virus. to explore whether recombination also plays a role in the evolution of the genomes of multipartite viruses, we developed a systematic approach that employs motif-finding tools to detect conserved motifs from divergent genomic regions and applies statistical approaches to select high-confidence motifs. the information th ... | 2013 | 23936517 |
| development of a test to assess the banana bunchy top virus transmissibility through direct analyze of its aphid vector pentalonia nigronervosa. | | 2013 | 23875297 |
| lack of evidence for an interaction between buchnera groel and banana bunchy top virus (nanoviridae). | circulative plant viruses such as luteovirids and geminiviruses have been shown to bind to groel proteins produced by endosymbiotic bacteria harboured within hemipteran vectors. these interactions seem to prevent the degradation of the viral particles in the aphid's haemocoel. similarly to luteovirids and geminiviruses, banana bunchy top virus (bbtv), a member of the nanoviridae family, is transmitted in a persistent, circulative manner and can be detected in the haemolymph of the aphid vector, ... | 2013 | 23816604 |
| high-throughput detection of banana bunchy top virus in banana plants and aphids using real-time taqman(®) pcr. | banana bunchy top virus (bbtv) is the causal agent of banana bunchy top disease. current diagnostic methods for bbtv are laborious and prone to generate false-negative results. a simple, reliable, and high-throughput method for detecting bbtv in plants and aphids has been developed, which involves tissues disruption from banana plants and viruliferous aphids followed by real-time taqman(®) pcr. extraction of bbtv single-stranded dna using this method is simple and less prone to contamination tha ... | 2013 | 23773808 |
| development of agrobacterium-mediated transformation of highly valued hill banana cultivar virupakshi (aab) for resistance to bbtv disease. | one of the most severe viral diseases of hill banana is caused by banana bunchy top virus (bbtv), a nanovirus transmitted by the aphid pentalonia nigronervosa. in this study, we reported the agrobacterium-mediated transformation on a highly valued hill banana cultivar virupakshi (aab) for resistance to bbtv disease. the target of the rna interference (rnai) is the rep gene, encoded by the bbtv-dna1. in order to develop rnai construct targeting the bbtv rep gene, the full-length rep gene of 870 b ... | 2013 | 23184576 |
| thermotherapy, chemotherapy, and meristem culture in banana. | bananas that provide a staple food to the millions of people are adversely affected by several viruses such as banana bunchy top virus (bbtv), banana streak virus (bsv), and cucumber mosaic virus (cmv). these viruses are known to have a devastating effect on crop production and constraint to the international exchange and conservation of banana germplasm-a cornerstone for breeding new cultivars. the viruses are particularly problematic in vegetative propagated crops, like bananas, because of the ... | 2013 | 23179717 |
| tropism, compartmentalization and retention of banana bunchy top virus (nanoviridae) in the aphid vector pentalonia nigronervosa. | plant viruses of the families luteoviridae and geminiviridae rely on hemipteran vectors for the infection of their hosts. several lines of evidence have revealed that these viruses are transmitted by competent vectors in a circulative manner, involving entry into the vector's body and the crossing of epithelial tissues forming the alimentary tract and the salivary glands. similar to luteovirids and geminiviruses, a third family of plant viruses, the family nanoviridae, have also been reported to ... | 2013 | 23015741 |
| predicting the benefits of banana bunchy top virus exclusion from commercial plantations in australia. | benefit cost analysis is a tried and tested analytical framework that can clearly communicate likely net changes in producer welfare from investment decisions to diverse stakeholder audiences. however, in a plant biosecurity context, it is often difficult to predict policy benefits over time due to complex biophysical interactions between invasive species, their hosts, and the environment. in this paper, we demonstrate how a break-even style benefit cost analysis remains highly relevant to biose ... | 2012 | 22879960 |
| rapid and sensitive detection of banana bunchy top virus by loop-mediated isothermal amplification. | a sensitive loop-mediated isothermal amplification (lamp) assay was developed for rapid detection of banana bunchy top virus (bbtv) infection. the reaction was performed in a single tube at 63°c for 90 min, with an improved closed-tube detection system by adding the sybr green i dye to the inside of the tube lid prior to amplification. the detection limit of the lamp assay was approximately 1 pg/μl plasmid dna when mixed with extracted dna from healthy banana plant, and no cross-reaction with ot ... | 2012 | 22771738 |
| transgenic banana plants expressing small interfering rnas targeted against viral replication initiation gene display high-level resistance to banana bunchy top virus infection. | the banana aphid-transmitted banana bunchy top virus (bbtv) is the most destructive viral pathogen of bananas and plantains worldwide. lack of natural sources of resistance to bbtv has necessitated the exploitation of proven transgenic technologies for obtaining bbtv-resistant banana cultivars. in this study, we have explored the concept of using intron-hairpin-rna (ihprna) transcripts corresponding to viral master replication initiation protein (rep) to generate bbtv-resistant transgenic banana ... | 2012 | 22552945 |
| cloning and sequence analysis of two banana bunchy top virus genomes in hainan. | the genome of banana bunchy top virus (bbtv) consists of six segments of single-stranded dna of approximately 1 kb in length. we identified and sequenced the complete genomes of two bbtv isolates, one with and one without satellite dna, from haikou, hainan, china. the haikou-2 isolate contains six genomic segments and an additional satellite dna while the haikou-4 isolate contains only six genomic segments. typical of other babuviruses, each genomic segment encodes a single open reading frame an ... | 2012 | 22286609 |
| evidence of inter-component recombination, intra-component recombination and reassortment in banana bunchy top virus. | banana bunchy top virus (bbtv; family nanoviridae, genus babuvirus) is a multi-component, ssdna virus, which causes widespread banana crop losses throughout tropical africa and australasia. we determined the full genome sequences of 12 bbtv isolates from the kingdom of tonga and analysed these together with previously determined bbtv sequences to show that reassortment and both inter- and intra-component recombination have all been relatively frequent occurrences during bbtv evolution. we found ... | 2012 | 22278830 |
| analysis of synonymous codon usage bias and phylogeny of coat protein gene in banana bract mosaic virus isolates. | the banana is one of the world's most important livelihood crops. banana plants are principally infected by four virus species, banana bunchy top virus (genus babuvirus), cucumber mosaic virus (genus cucumovirus), banana streak virus (genus badnavirus) and banana bract mosaic virus (genus potyvirus). the objective of this study is to understand the codon usage pattern and phylogeny of coat protein gene in different banana bract mosaic virus (bbrmv) isolates. the bbrmv coat protein (cp) gene was ... | 2017 | 28770241 |
| cloning of bbtv (banana bunchy top virus) components and screening of bbtv using functionalized gold nanoparticles. | banana bunchy top virus (bbtv) affects all varieties of banana plants and causes heavy economic loss in most of the banana cultivating areas. the bbtv genome comprises of six dna components; in this study, we have cloned the six bbtv-dna components from one of the bbtv-infected plants (tri-8) and were submitted to genbank. analysis of the bbtv dna-r component showed that it belonged to south pacific group. resistance against bbtv has not been observed so far in banana plants and removal and kill ... | 2017 | 28677087 |
| genetic diversity of banana bunchy top virus isolates from china. | banana bunchy top virus (bbtv) (the genus babuvirus, the family nanoviridae) is a single-stranded circular dna virus with a genome composed of six components designated as dna-r, -u3, -s, -m, -c, and -n. this study analyzed the nucleotide identities of the dna-r of 23 isolates from banana-producing provinces of china, including guangdong, hainan, guangxi, and yunnan. results showed that the nucleotide identity of dna-r was 72.3-100%. phylogenetic analysis indicated that these bbtv isolates were ... | 2017 | 28523929 |
| editing plants for virus resistance using crispr-cas. | this minireview summarizes recent advancements using the clustered regularly interspaced palindromic repeats-associated nuclease systems (crispr-cas) derived from prokaryotes to breed plants resistant to dna and rna viruses. the crispr-cas system represents a powerful tool able to edit and insert novel traits into plants precisely at chosen loci offering enormous advantages to classical breeding. approaches to engineering plant virus resistance in both transgenic and non-transgenic plants are di ... | 2017 | 28523919 |
| immunofluorescence localisation of banana bunchy top virus (family nanoviridae) within the aphid vector, pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteoviruses. | we have applied immunocapture pcr and developed an immunofluorescence assay to specifically detect banana bunchy top virus (bbtv; family nanoviridae, genus babuvirus) within its aphid vector, pentalonia nigronervosa (hemiptera, aphididae). bbtv was localised using either monoclonal or polyclonal antibodies into the anterior midgut (stomach) and into specific cells forming the principal salivary glands. these results suggest a distinct path of virus translocation that likely differs from the one ... | 2010 | 21167229 |
| molecular characterization of geographically different banana bunchy top virus isolates in india. | banana bunchy top disease (bbtd) caused by banana bunchy top virus (bbtv) is one of the most devastating diseases of banana and poses a serious threat for cultivars like hill banana (syn: virupakshi) and grand naine in india. in this study, we have cloned and sequenced the complete genome comprised of six dna components of bbtv infecting hill banana grown in lower pulney hills, tamil nadu state, india. the complete genome sequence of this hill banana isolate showed high degree of similarity with ... | 2011 | 23637489 |
| molecular characterization of an indian isolate of banana bunchy top virus based on six genomic dna components. | banana bunchy top virus (bbtv) is a single-stranded circular dna virus of the genus babuvirus, belonging to family nanoviridae. the six genomic dna components of indian (lucknow) isolate of bbtv were amplified by polymerase chain reaction (pcr) with specific primers using total dna extracted from banana tissues showing typical symptoms of banana bunchy top disease (bbtd). the resulting ~1.1 kb amplicons were cloned and sequenced. analysis of sequence data revealed the presence of six full-length ... | 2009 | 19194792 |
| development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars. | recombinase polymerase amplification (rpa) is a rapid, isothermal amplification method with high specificity and sensitivity. in this study, an assay was developed and evaluated for the detection of banana bunchy top virus (bbtv) in infected banana plants. three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of bbtv to function both in rpa as well as in polymerase chain reaction (pcr). a total of 133 symptomatic as well as asymptomatic banana leaf ... | 2017 | 28500444 |