| characterization of an avian rotavirus strain by neutralization and molecular hybridization assays. | pigeon rotavirus strain po-13, which was recently shown to be neutralized by a hyperimmune serum to the prototype serotype 7 virus ch-2, showed a one-way neutralization cross with turkey rotavirus ty-1. when its genome was compared by rna-rna hybridization under stringent conditions with those of avian and mammalian rotaviruses, po-13 displayed a low to medium level of homology only with turkey rotavirus strains ty-1 and ty-3 but not with chicken rotavirus strain ch-1. furthermore, no homology w ... | 1991 | 1665575 |
| nucleotide and amino acid sequence analysis of the rotavirus nonstructural rna-binding protein ns35. | ns35, a basic protein encoded by gene 8 of sa11 rotavirus, possesses rna-binding activity and is essential for genome replication. to identify conserved regions in the ns35 gene and its protein product, we determined the nucleotide sequences of the ns35 gene for the mammalian and avian rotaviruses wa, ds1, sa11 (patton and ramig strains), ncdv, and ty-1 and compared them and their deduced amino acid sequences to those reported for sa11 (both strain), osu, and uk. the results indicated that the n ... | 1993 | 8380660 |
| the avian rotavirus ty-1 vp7 nucleotide and deduced amino acid sequences differ significantly from those of ch-2 rotavirus. | the vp7 gene of the avian strain ty-1, which is classified as g7 serotype, was sequenced and the amino acid sequence deduced. the gene is 1065 nucleotides long with a long open reading frame of 987 nucleotides producing a protein 329 amino acids in length. the amino acid homology of the ty-1 vp7 protein to that of the avian ch-2 vp7 was 70%. the a, b, and c variable epitope regions of ty-1 were unique compared to those of ch-2 and other strains representing the 14g serotypes. the low 53% homolog ... | 1993 | 8385915 |