| transforming variants of the avian myc-containing retrovirus fh3 arise prior to phenotypic selection. | the avian retrovirus fh3, which encodes a gag-myc fusion protein, transforms chicken macrophages but not fibroblasts. however, passage of fh3 viral stock in fibroblasts leads to emergence of a virus capable of fibroblast transformation. this virus has not acquired myc mutations; instead, it carries internal gag deletions which confer the ability to transform fibroblasts. we now demonstrate that this and similar deletion variants emerge repeatedly during selection. sequence analysis reveals direc ... | 1993 | 8497071 |
| gag as well as myc sequences contribute to the transforming phenotype of the avian retrovirus fh3. | the avian retrovirus fh3, like mc29 and cmii, encodes a gag-myc fusion protein. however, the fh3-encoded protein is larger, about 145 kda, and contains almost the entire retroviral gag gene. in contrast to the other gag-myc avian retroviruses, fh3 fails to transform fibroblasts in vitro, although macrophages are transformed both in vitro and in vivo (c. chen, b. j. biegalke, r. n. eisenman, and m. l. linial, j. virol. 63:5092-5100, 1989). we have used the polymerase chain reaction technique to o ... | 1992 | 1731115 |