| isolation of a subterranean clover mottle virus-like satellite rna from lucerne infected with lucerne transient streak virus. | a circular, viroid-like satellite rna (sat rna) was detected in lucerne transient streak virus (ltsv), from lucerne in south australia. it was larger than the previously reported sat rna of ltsv, being similar in size and sequence homology to the 388 nucleotide sat rna previously shown to be encapsidated by subterranean clover mottle virus (scmov) isolated from subterranean clover in western australia. this indicates that under field conditions, very similar sat rnas can be associated with two d ... | 1990 | 1697331 |
| single antibody dot immunoassay--a simple technique for rapid detection of a plant virus. | a single antibody dot immunoassay (sadi) was developed for use as a rapid, simple and sensitive technique for the direct assay of virus bound to nitrocellulose membranes. sadi has been tested for the detection of subterranean clover mottle virus (scmov) in small amounts of infected tissue and purified virus preparations, and the technique was found to be twelve times more sensitive than elisa in terms of total antigen detected. diba, an indirect dot immunoassay, was about twice as sensitive as s ... | 1986 | 3722309 |
| using epidemiological information to develop effective integrated virus disease management strategies. | virus diseases cause serious losses in yield and quality of cultivated plants worldwide. these losses and the resulting financial damage can be limited by controlling epidemics using measures that minimise virus infection sources or suppress virus spread. for each combination of virus, cultivated plant and production system, there is an 'economic threshold' above which the financial damage is sufficient to justify using such measures. however, individual measures used alone may bring only small ... | 2004 | 15036832 |
| the complete nucleotide sequence of subterranean clover mottle virus. | the complete nucleotide sequence of subterranean clover mottle virus (scmov) genomic rna has been determined. the scmov genome is 4,258 nucleotides in length. it shares most nucleotide and amino acid sequence identity with the genome of lucerne transient streak virus (ltsv). scmov rna encodes four overlapping open reading frames and has a genome organisation similar to that of cocksfoot mottle virus (cfmv). orf1 and orf4 are predicted to encode single proteins. orf2 is predicted to encode two pr ... | 2003 | 14579180 |
| a stable hammerhead structure is not required for endonucleolytic activity of a ribozyme in vivo. | cleavage of a specific target, the mrna encoding the bacterial neomycin phosphotransferase, by mutant satellite rna of subterranean clover mottle virus (sscmov) ribozymes (rz) was used to study the role of the hammerhead (hh) structure in rz activity in cis and in trans. the bimolecular rz-target rna interaction was predicted by computer secondary structure analysis. in vivo, endonucleolytic cleavage was determined in plant protoplasts and compared with in vitro results. two point mutations with ... | 1994 | 7958987 |
| structure, self-cleavage, and replication of two viroid-like satellite rnas (virusoids) of subterranean clover mottle virus. | both the genomic and viroid-like satellite rnas (virusoids) from four subterranean clover mottle virus isolates described by francki et al. were analyzed in detail. restriction endonuclease mapping of cdnas prepared from the genomic rnas from all isolates showed that these rnas are closely related if not identical. the two virusoids, which can occur together in the same isolate or individually, were sequenced and shown to be able to form highly base-paired viroid-like secondary structures. the l ... | 1990 | 1693803 |