| sequence similarity between xylose isomerase and replicase: another tim-barrel in the replicase structure? | the blast search using the strand beta 2 (46_gahgvtfhdddlip) of the (alpha/beta)8-barrel of xylose isomerase from streptomyces olivochromogenes resulted in retrieving the sequentially similar segment of replicase from garlic latent virus (692_gghgigfhrdd). the detailed analysis of the entire amino acid sequences of both xylose isomerase and replicase suggested that the polypeptide segment 644-1046 of replicase (the entire length of this enzyme is 1924 residues) could share the structure of xylos ... | 1997 | 9352374 |
| differentiation of allium carlaviruses isolated from different parts of the world based on the viral coat protein sequence. | common primers which amplify the 3' terminal genomic rnas of allium carlaviruses were designed based on the nucleotide sequence of shallot latent virus (slv), garlic latent virus (glv) and garlic common latent virus (gclv). a total of fifteen cdnas encoding the coat protein (cp) of the carlaviruses, including the biologically identified isolates slv, glv and gclv as well as viruses from infected allium plants cultivated in different parts of the world, were amplified by rt-pcr with the common pr ... | 1998 | 9687867 |
| molecular characterisation of a complex mixture of viruses in garlic with mosaic symptoms in china. | degenerate primers were used to detect and amplify cdna of viruses of the genera carlavirus, allexivirus and potyvirus from garlic plants with mosaic symptoms growing in zhejiang province, china. plants contained a complex mixture of viruses and strains. three distinct stains of garlic latent virus were detected; the most frequent one was completely sequenced and partial sequences were obtained for the other two. the complete sequence (8363 nt) was 76.4% identical to a korean isolate. two allexi ... | 2001 | 11722009 |
| characterisation of some carla- and potyviruses from bulb crops in china. brief report. | conserved carla- and potyvirus primers were used in rt-pcr to amplify virus fragments from garlic and other bulb crops in china and the fragments were subsequently sequenced and compared in phylogenetic analyses. garlic plants from henan, hubei, jiangsu, shangdong and yunnan provinces all contained at least one isolate each of garlic latent virus (genus carlavirus), onion yellow dwarf virus (oydv, genus potyvirus) and leek yellow stripe virus (lysv, genus potyvirus). the complete sequence of a z ... | 2002 | 11890533 |
| complete genome sequence of garlic latent virus, a member of the carlavirus family. | the complete genome sequence of the garlic latent virus (glv) has been determined. the whole glv genome consists of 8,353 nucleotides, excluding the 3'-end poly(a)+ tail, and contains six open-reading frames (orfs). putative proteins that were encoded by the reading frames contain the motifs that were conserved in carlavirus-specific rna replicases, ntp-dependent dna helicases, two viral membrane-bound proteins, a viral coat protein, and a zinc-finger. overall, the glv genome shows structural fe ... | 2002 | 12442892 |
| [cloning and expression of two garlic virus coat protein genes]. | the coat protein(cp) genes of garlic mosaic virus(gmvc) and garlic latent virus(glvc) isolated from garlic(allium) plants in tianjin, china, were amplified from an established cdna library by pcr method and subsequently expressed in e. coli. using the pet-30a expression system. the determined sequences of gmvc and glvc cp genes show that the complete gmvc cp gene has 867 nucleotides encoding 289 amino acids. it has 88.5% and 97.2% homology, at the levels of nucleotide and amino acid, respectivel ... | 2001 | 12552905 |
| genome organization and variation in the 3'-partial sequence of garlic latent virus in china. | ten different isolates of a carlavirus were detected by degenerate pcr from 12 garlic samples collected from 6 provinces in china, and the complete genome sequence of the zhejiang isolate zj1 and 3'-terminal sequences of 9 other isolates were determined. the rna genome of isolate zj1 consisted of 8363nts excluding the 3'-poly (a) tail, and the genome organization was similar to other carlaviruses with 6 open reading frames encoding a replicase, tgb1, tgb2, tgb3, cp and nabp respectively. sequenc ... | 2002 | 18759032 |