| the nucleotide sequence of rna-1 of raspberry bushy dwarf virus. | raspberry bushy dwarf virus (rbdv) has isometric, 33 nm diameter particles and a bipartite rna genome. sequencing of the larger component (rna-1) showed that it consists of 5449 nucleotides and contains one large open reading frame encoding a putative translation product with a calculated m(r) of 190,000. comparisons of this polypeptide with non-structural proteins of other plant viruses revealed significant homologies with those of alfalfa mosaic virus (almv), brome mosaic virus (bmv), cucumber ... | 1992 | 1469359 |
| nucleotide sequence of raspberry bushy dwarf virus rna-2: a bicistronic component of a bipartite genome. | northern blot analysis with cdna probes to rna-3 (1 kb) of raspberry bushy dwarf virus (rbdv) revealed extensive sequence homology with rbdv rna-2 (2.2 kb). nucleotide sequencing showed that rna-2 contains two large open reading frames (orfs), of 1074 (5' orf) and 822 (3' orf) bases. the 3' orf is virtually identical in sequence to rna-3, which encodes the mr 30509 (30k) coat protein. the 5' orf encodes an mr 38860 (39k) protein which slightly resembles the 32k protein encoded by rna-3 of alfalf ... | 1991 | 1895055 |
| nucleotide sequence of raspberry bushy dwarf virus rna-3. | a nucleotide sequence is reported for rna-3, the smallest of the three major rna species found in particles of raspberry bushy dwaft virus (rbdv). the sequence of 946 nucleotides contains a single large open reading frame which encodes an mr 30,509 polypeptide. in vitro translation of rna-3 yielded an mr 30,000 product that reacted specifically with antiserum to rbdv particles and we conclude that the amino acid sequence deduced from the sequence of rna-3 is that of the rbdv coat protein, or an ... | 1991 | 1993886 |
| proposed classification of the bipartite-genomed raspberry bushy dwarf idaeovirus, with tripartite-genomed viruses in the family bromoviridae. | raspberry bushy dwarf virus (rbdv) has an unusual combination of properties and has been classified as the sole member of a new plant virus genus, for which the name idaeovirus has been proposed. particles of rbdv resemble those of ilarviruses (family bromoviridae) in appearance and in being transmitted in association with pollen. rbdv has two genomic rna species, rna-1 (5,449 nt) and rna-2 (2,231 nt). the particles also contain rna-3 (946 nt), a subgenomic monocistronic coat protein mrna which ... | 1993 | 8347087 |
| rapid differentiation of closely related isolates of two plant viruses by polymerase chain reaction and restriction fragment length polymorphism analysis. | immunocapture reverse transcriptase-polymerase chain reaction (rt-pcr) followed by restriction fragment length polymorphism (rflp) analysis of the product has been shown to be an effective procedure for discriminating serologically indistinguishable isolates of two plant viruses, raspberry bushy dwarf (rbdv) and zucchini yellow mosaic (zymv). for both viruses, only limited sequence information was available at the time of primer design, but most of the isolates which were tested could be amplifi ... | 1995 | 8576302 |
| single-step immunocapture rt-pcr in the detection of raspberry bushy dwarf virus. | an immunocapture reverse transcription polymerase chain reaction (ic-rt-pcr) method for a highly sensitive analysis of raspberry bushy dwarf virus (rbdv) in infected plants is described. in the method, preliminary purification of virus particles or viral rna from the plant material is not necessary. viruses are enriched during the assay by antibodies bound in the pcr microplate wells, followed by lysis of the viral particles, and rt-pcr of the viral rna. the reaction mixtures, including reverse ... | 1996 | 8723929 |
| transmission of raspberry bushy dwarf virus by seed of chenopodium quinoa. | | 1998 | 9842450 |
| identificaton of raspberry bushy dwarf virus strains occurring in the czech republic. | | 1999 | 10757236 |
| comparison of sugar, acids, and volatile composition in raspberry bushy dwarf virus-resistant transgenic raspberries and the wild type 'meeker' (rubus idaeus l.). | raspberry bushy dwarf virus (rbdv) causes a significant reduction in yield and quality in raspberry and raspberry-blackberry hybrid. genetic modifications were made to 'meeker' red raspberries to impart rbdv resistance. the rbdv-resistant transgenic and wild type 'meeker' plants were grown in oregon and washington, and the fruits were harvested in the 2004 and 2005 growing seasons. year-to-year and site-to-site variations were observed for the degrees brix and titratable acidity, with oregon ras ... | 2008 | 18598047 |
| combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral rna degradation in shoot tips. | accumulation of viruses in vegetatively propagated plants causes heavy yield losses. therefore, supply of virus-free planting materials is pivotal to sustainable crop production. in previous studies, raspberry bushy dwarf virus (rbdv) was difficult to eradicate from raspberry (rubus idaeus) using the conventional means of meristem tip culture. as shown in the present study, it was probably because this pollen-transmitted virus efficiently invades leaf primordia and all meristematic tissues excep ... | 2008 | 18705855 |
| genome activation by raspberry bushy dwarf virus coat protein. | two sets of infectious cdna clones of raspberry bushy dwarf virus (rbdv) have been constructed, enabling either the synthesis of infectious rna transcripts or the delivery of infectious binary plasmid dna by infiltration of agrobacterium tumefaciens. in whole plants and in protoplasts, inoculation of rbdv rna1 and rna2 transcripts led to a low level of infection, which was greatly increased by the addition of rna3, a subgenomic rna coding for the rbdv coat protein (cp). agroinfiltration of rna1 ... | 2009 | 19218221 |
| a novel plant virus with unique properties infecting japanese holly fern. | a novel rna virus with a bipartite genome has been found associated with an emerging disease affecting japanese holly fern (cyrtomium falcatum). diseased japanese holly fern plants showed a variety of foliar symptoms and reduction in size. the virus was transmitted by grafting, as well as through spores from an infected plant. partially purified preparations of the virus from infected ferns contained quasi-spherical particles that ranged from 30 to 40 nm in diameter. double-stranded rna (dsrna) ... | 2009 | 19570959 |
| improved recovery of cryotherapy-treated shoot tips following thermotherapy of in vitro-grown stock shoots of raspberry (rubus idaeus l.). | raspberry bushy dwarf virus (rbdv) can be efficiently eradicated from raspberry plants (rubus idaeus) by a procedure combining thermotherapy and cryotherapy. however, the bottleneck of this procedure is that, following thermotherapy, cryopreserved shoot tips become chlorotic during regrowth and eventually die after several subcultures. in addition, survival of heat-treated stock shoots and recovery of cryopreserved shoot tips following thermotherapy are low. the present study focused towards imp ... | 2009 | 19750241 |
| protein kinase c-dependent phosphorylation of borna disease virus p protein is required for efficient viral spread. | mutational analysis of the phosphate acceptor sites of the borna disease virus (bdv) phosphoprotein (p) has suggested a role of phosphorylation for viral spread. however, the studied mutant viruses also had two amino acid exchanges in the x protein, because the reading frames of p and x overlap. to determine the relative contribution of p and x to viral attenuation, we studied a p variant with serine-to-leucine substitutions (p(s26l,s28l)) in which the wild-type x sequence was conserved. viral s ... | 2010 | 20333534 |
| molecular characterisation of raspberry bushy dwarf virus isolates from sweden and belarus. | the complete coding sequences were determined for rna-1 and rna-2 of five raspberry isolates of raspberry bushy dwarf virus (rbdv) from belarus (by1, by3, by8, by22) and sweden (se3). the analysed sequences for both rna-1 and rna-2 were highly conserved among these isolates. phylogenetic analyses including available sequences for the cp gene and the mp gene showed that all analysed rbdv isolates from raspberry were closely related. however, there was no strong correlation between the grouping of ... | 2011 | 21253783 |
| Real-time RT-PCR for detection of Raspberry bushy dwarf virus, Raspberry leaf mottle virus and characterizing synergistic interactions in mixed infections. | Two TaqMan-based real-time One-Step RT-PCR assays were developed for the rapid and efficient detection of Raspberry bushy dwarf virus (RBDV) and Raspberry leaf mottle virus (RLMV), two of the most common raspberry viruses in North America and Europe. The primers and probes were designed from conserved fragments of the polymerase region of each virus and were effective for the detection of different isolates tested in this study. The RBDV assay amplified a 94bp amplicon and was able to detect as ... | 2012 | 21968094 |
| A novel borna disease virus vector system that stably expresses foreign proteins from an intercistronic noncoding region. | Borna disease virus (BDV), a nonsegmented, negative-strand RNA virus, infects a wide variety of mammalian species and readily establishes a long-lasting, persistent infection in brain cells. Therefore, this virus could be a promising candidate as a novel RNA virus vector enabling stable gene expression in the central nervous system (CNS). Previous studies demonstrated that the 5' untranslated region of the genome is the only site for insertion and expression of a foreign gene. In this study, we ... | 2011 | 21937656 |
| complete genome sequence and analysis of blackcurrant leaf chlorosis associated virus, a new member of the genus idaeovirus. | blackcurrant leaf chlorosis associated virus (bclcav) was isolated from symptomatic blackcurrants (ribes nigrum cv. baldwin). the virus has a genome organization similar to that of raspberry bushy dwarf virus (rbdv), the type member of the genus idaeovirus. the rna-1of this virus encodes the replicase complex (orf1, mr 197 kda), while rna-2 encodes a putative movement protein (orf2a, mr 38.8 kda) and the putative coat protein (orf2b, mr 30 kda). a concatenated form of bclcav rna-2 was detected b ... | 2017 | 28190194 |
| identification and characterization of privet leaf blotch-associated virus, a novel idaeovirus. | a novel virus has been identified by next-generation sequencing (ngs) in privet (ligustrum japonicum l.) affected by a graft-transmissible disease characterized by leaf blotch symptoms resembling infectious variegation, a virus-like privet disease with an unclear aetiology. this virus, which has been tentatively named 'privet leaf blotch-associated virus' (prlbav), was absent in non-symptomatic privet plants, as revealed by ngs and reverse transcription-polymerase chain reaction (rt-pcr). molecu ... | 2016 | 27349357 |
| pollen tubes introduce raspberry bushy dwarf virus into embryo sacs during fertilization processes. | we developed a fertilization method in which pollen tubes entered into embryo sacs without any need to contact surrounding female sporophytic cells by using torenia fournieri (torenia) plants under the condition of hindering movement of the virus from a stigma, which is the first infection site leading to systemic infection. when rbdv-infected torenia pollen grains were used for the developed fertilization method, the virus was transmitted to the seeds by pollen tubes germinating from them. on t ... | 2015 | 26176979 |
| a raspberry bushy dwarf virus isolate from ecuadorean rubus glaucus contains an additional rna that is a rearrangement of rna-2. | sequencing of the complete genome of a raspberry bushy dwarf virus isolate from rubus glaucus in ecuador revealed that its rna-1 and rna-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from sweden and slovenia. in dsrna analysis of infected plants, an additional band of 3 kbp was observed. sequencing of this band revealed that it was 3279 nt long. blast searches revealed that this band contained a modified version of rna-2, which consisted of rn ... | 2014 | 24719196 |
| penetration of pollen tubes with accumulated raspberry bushy dwarf virus into stigmas is involved in initial infection of maternal tissue and horizontal transmission. | torenia fournieri (torenia) plants were infected with raspberry bushy dwarf virus (rbdv) by pollination with rbdv-infected raspberry pollen grains. the infected raspberry pollen grains germinated on torenia stigmas, and then the pollen tubes penetrated into the stigma, even though the pollen tubes were arrested in the styles. in whole-mount in situ hybridization of germinating infected raspberry pollen grains, rbdv accumulated in the tips of the pollen tubes. tissue blot hybridization of torenia ... | 2014 | 24606702 |