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electron microscopy of the novel barley yellow streak mosaic virus.unique particles of barley yellow streak mosaic virus (bysmv) were detected in diseased barley, wheat, and several species of grass. they appeared to be about 64 nm in width and from 127 nm to an astonishing 4000 nm in length. individual particles were circular in transverse section. the outermost layer of each particle seemed to be a membrane-like envelope. the internal structure of many particles was bead-like. some particles had centers that were translucent. the bysmv particles were distribu ...19892635708
investigations on the structure and morphogenesis of some arboviruses isolated in the u.s.s.r.the morphology and some stages of morphogenesis in suckling mouse brains of hitherto serologically unclassified viruses kaspiy (leiv-a-63), zavashan (leiv-ap-6158), artashat (leiv-a-2366), and paramushir (leiv-c-2268), isolated in the u.s.s.r., of gm-710 virus isolated in scotland, and of sokuluk (leiv-k-400) virus belonging to the genus flavivirus (family togaviridae) were studied. virion sizes were determined and changes in infected cells described. based on their structure and morphogenesis, ...19816115562
unconventional agents and unclassified viruses: recent advances in biology and epidemiology. proceedings of the 8th munich symposium on microbiology. munich, october 21-22, 1992. 19938106030
structure, function, and evolution of adenovirus-associated rna: a phylogenetic approach.to explore the structure and function of a small regulatory rna, we examined the virus-associated (va) rna species of all 47 known human adenovirus serotypes and of one simian virus, sa7. the va rna gene regions of 43 human adenoviruses were amplified and sequenced, and the structures of 10 representative va rnas were probed by nuclease sensitivity analysis. most human viruses have two va rna species, va rna, and va rnaii, but nine viruses (19%) have a single va rna gene. sequence alignments cla ...19968764016
phylogeny of the genus flavivirus.we undertook a comprehensive phylogenetic study to establish the genetic relationship among the viruses of the genus flavivirus and to compare the classification based on molecular phylogeny with the existing serologic method. by using a combination of quantitative definitions (bootstrap support level and the pairwise nucleotide sequence identity), the viruses could be classified into clusters, clades, and species. our phylogenetic study revealed for the first time that from the putative ancesto ...19989420202
international committee on taxonomy of viruses and the 3,142 unassigned species.in 2005, ictv (international committee on taxonomy of viruses), the official body of the virology division of the international union of microbiological societies responsible for naming and classifying viruses, will publish its latest report, the state of the art in virus nomenclature and taxonomy. the book lists more than 6,000 viruses classified in 1,950 species and in more than 391 different higher taxa. however, genbank contains a staggering additional 3,142 "species" unaccounted for by the ...200516105179
complete nucleotide sequence of four rna segments of fig mosaic virus.the complete sequence of four viral rna segments of fig mosaic virus (fmv) was determined. each of the four rnas comprises a single open reading frame (orf) 7,093, 2,252, 1,490 and 1,472 nucleotides in size, respectively. these orfs encode the following proteins in the order: rna-dependent rna polymerase (p1 264 kda), a putative glycoprotein (p2 73 kda), a putative nucleocapsid protein (p3 35 kda) and a protein with unknown function (p4 40.5 kda). all rna segments possess untranslated regions co ...200919777155
Genome-based phylogeny of dsDNA viruses by a novel alignment-free method.Sequence alignment is not directly applicable to whole genome phylogeny since several events such as rearrangements make full length alignments impossible. Here, a novel alignment-free method derived from the standpoint of information theory is proposed and used to construct the whole-genome phylogeny for a population of viruses from 13 viral families comprising 218 dsDNA viruses. The method is based on information correlation (IC) and partial information correlation (PIC). We observe that (i) t ...201222100880
aguacate virus, a new antigenic complex of the genus phlebovirus (family bunyaviridae).genomic and antigenic characterization of aguacate virus, a tentative species of the genus phlebovirus, and three other unclassified viruses, armero virus, durania virus and ixcanal virus, demonstrate a close relationship to one another. they are distinct from the other nine recognized species within the genus phlebovirus. we propose to designate them as a new (tenth) serogroup or species (aguacate virus) within the genus. the four viruses were all isolated from phlebotomine sandflies (lutzomyia ...201121325481
quaranfil, johnston atoll, and lake chad viruses are novel members of the family orthomyxoviridae.arboviral infections are an important cause of emerging infections due to the movements of humans, animals, and hematophagous arthropods. quaranfil virus (qrfv) is an unclassified arbovirus originally isolated from children with mild febrile illness in quaranfil, egypt, in 1953. it has subsequently been isolated in multiple geographic areas from ticks and birds. we used high-throughput sequencing to classify qrfv as a novel orthomyxovirus. the genome of this virus is comprised of multiple rna se ...200919726499
[complete genome sequence analysis of a newly isolated paramyxovirus tianjin strain].paramyxovirus tianjin strain is the high-pathogenic virus to primate and might also cause human lower respiratory tract infection. to determine the genome structure, variation features and phylogenetic position, the complete nucleotide sequence of paramyxovirus tianjin strain was analyzed. the homology comparison and phylogenetic analysis of the nucleotide and the deduced amino acid sequences among paramyxovirus tianjin strain and the 28 strains in seven genera and the 7 unclassified viruses of ...200818320815
whole genome molecular phylogeny of large dsdna viruses using composition vector method.one important mechanism by which large dna viruses increase their genome size is the addition of modules acquired from other viruses, host genomes or gene duplications. phylogenetic analysis of large dna viruses, especially using methods based on alignment, is often difficult due to the presence of horizontal gene transfer events. the recent composition vector approach, not sensitive to such events, is applied here to reconstruct the phylogeny of 124 large dna viruses.200717359548
systematics of rna tumor viruses and virus-like particles of human origin.[(3)h]dna copies of avian, feline, murine, and primate rna tumor virus genomes were synthesized in vitro by an rna-dependent dna polymerase reaction. these dnas were hybridized to 60-70s rna that had been purified from the viruses. the amount of the [(3)h]dna hybridized yielded a measure of the genetic relatedness among the dna preparations synthesized by the viruses. when many combinations of dna and rna were analyzed, the pattern of hybridization showed in some cases that the dna copies of the ...19744137960
electron microscopic and antigenic studies of uncharacterized viruses. iii. evidence suggesting the placement of viruses in the family reoviridae.this is the last of three papers describing the use of electron microscopy and antigenic tests intended to characterize and place in taxa more than 60 previously unclassified viruses. the first paper describes the viruses we classified as members of the families arenaviridae, paramyxoviridae, or poxviridae. the second paper describes those classified as members of the virus family bunyaviridae. another paper, published separately, discusses viruses classified as members of the family rhabdovirid ...19892692538
electron microscopic and antigenic studies of uncharacterized viruses. ii. evidence suggesting the placement of viruses in the family bunyaviridae.this is the second of three papers describing the use of electron microscopy and antigenic analyses intended to characterize and place in taxa more than 60 previously unclassified viruses. the first paper of the series describes the viruses we classified as provisional members of the families arenaviridae, paramyxoviridae, or poxviridae; another paper, published separately, discusses the rhabdoviridae. in this paper we report that electron microscopy provided sufficient evidence to place 17 of t ...19892690776
whole-proteome phylogeny of large dsdna viruses and parvoviruses through a composition vector method related to dynamical language model.the vast sequence divergence among different virus groups has presented a great challenge to alignment-based analysis of virus phylogeny. due to the problems caused by the uncertainty in alignment, existing tools for phylogenetic analysis based on multiple alignment could not be directly applied to the whole-genome comparison and phylogenomic studies of viruses. there has been a growing interest in alignment-free methods for phylogenetic analysis using complete genome data. among the alignment-f ...201020565983
whole-proteome phylogeny of large dsdna virus families by an alignment-free method.the vast sequence divergence among different virus groups has presented a great challenge to alignment-based sequence comparison among different virus families. using an alignment-free comparison method, we construct the whole-proteome phylogeny for a population of viruses from 11 viral families comprising 142 large dsdna eukaryote viruses. the method is based on the feature frequency profiles (ffp), where the length of the feature (l-mer) is selected to be optimal for phylogenomic inference. we ...200919553209
phylogenetic relationships of seven previously unclassified viruses within the family rhabdoviridae using partial nucleoprotein gene sequences.partial nucleoprotein (n) gene sequences of the rhabdoviruses obodhiang (obov), kotonkon (kotv), rochambeau (rbuv), kern canyon (kcv), mount elgon bat (mebv), kolongo (kolv) and sandjimba (sjav) were generated and their phylogenetic positions within the family rhabdoviridae were determined. both obov and kotv were placed within the genus ephemerovirus. rbuv was joined to the same cluster, but more distantly. mebv and kcv were grouped into a monophyletic cluster (putative genus) with oita virus ( ...200616847128
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