| site-specific integration of the temperate bacteriophage phi adh into the lactobacillus gasseri chromosome and molecular characterization of the phage (attp) and bacterial (attb) attachment sites. | the temperate bacteriophage phi adh integrates its genome into the chromosomal dna of lactobacillus gasseri adh by a site-specific recombination process. southern hybridization analysis of bcli-digested genomic dna from six relysogenized derivatives of the prophage-cured strain nck102 displayed phage-chromosomal junction fragments identical to those of the lysogenic parent. the phi adh attachment site sequence, attp, was located within a 365-bp ecori-hindiii fragment of phage phi adh. this fragm ... | 1992 | 1512192 |
| genetic transfer systems for delivery of plasmid deoxyribonucleic acid to lactobacillus acidophilus adh: conjugation, electroporation, and transduction. | lactobacillus acidophilus adh is a bacteriocin-producing human isolate that adheres to human fetal intestinal cells and human ileal cells. we have employed both electroporation and conjugation methodologies to transfer various plasmids to l. acidophilus adh. furthermore, we have demonstrated transduction of plasmid dna within this strain by a temperate bacteriophage (phi adh) harbored by l. acidophilus adh. plasmid pgk12 was introduced into strain adh by electroporation at frequencies as high as ... | 1989 | 2503549 |
| characterization of the temperate bacteriophage phi adh and plasmid transduction in lactobacillus acidophilus adh. | lactobacillus acidophilus adh is lysogenic and harbors an inducible prophage, phi adh. bacteriophage were detected in cell lysates induced by treatment with mitomycin c or uv light. electron microscopy of lysates revealed phage particles with a hexagonal head (62 nm) and a long, noncontractile, flexible tail (398 nm) ending in at last five short fibers. phage phi adh was classified within bradley's b1 phage group and the siphoviridae family. the phi adh genome is a linear double-stranded dna mol ... | 1989 | 2508554 |
| primary structure and functional analysis of the lysis genes of lactobacillus gasseri bacteriophage phi adh. | the lysis genes of the lactobacillus gasseri bacteriophage phi adh were isolated by complementation of a lambda sam mutation in escherichia coli. nucleotide sequencing of a 1,735-bp dna fragment revealed two adjacent coding regions of 342 bp (hol) and 951 bp (lys) in the same reading frame which appear to belong to a common transcriptional unit. proteins corresponding to the predicted gene products, holin (12.9 kda) and lysin (34.7 kda), were identified by in vitro and in vivo expression of the ... | 1995 | 7836307 |
| genetic organization and sequence of the region encoding integrative functions from lactobacillus gasseri temperate bacteriophage phi adh. | a 2.0-kb fragment from the lactobacillus gasseri temperate bacteriophage phi adh contained the essential genetic determinants for site-specific integration. the nucleotide sequence of this fragment was determined. an open reading frame (intg), which adjoined the phage attachment site (attp), encoded a deduced protein related to the integrase family. the organization of this region was comparable to other phage site-specific recombination systems. | 1993 | 8472961 |
| structure of a genome region of the lactobacillus gasseri temperate phage phiadh covering a repressor gene and cognate promoters. | by sequencing the dna regions which flank the intg gene encoding integrase of the temperate lactobacillus (lb.) gasseri bacteriophage phiadh, a continuous sequence of 6590 bp was established. it encompasses five newly identified orfs, of which four are located upstream, and one (orfc) downstream of intg. proteins corresponding to the expected products of the intg upstream coding regions, orfa (33 kda), orf2 (14 kda), rad (12.1 kda), and tec (7.9 kda), were identified by in vitro expression of su ... | 1998 | 9599081 |
| primary structure and features of the genome of the lactobacillus gasseri temperate bacteriophage (phi)adh. | the complete dna sequence of the lactobacillus (lb.) gasseri temperate phage (phi)adh was determined. the linear and double-stranded genome consists of 43.785bp with a g+c content of 35. 3% and 3' protruding cohesive ends of 12nt. sixty-two possible orfs were identified. on the basis of homology comparisons, some of them could be assigned to possible functions, such as a helicase, a nucleic acid polymerase and a protease. in a non-coding area of the (phi)adh genome, structural features of a pote ... | 1999 | 10452953 |
| transcript map of the temperate lactobacillus gasseri bacteriophage phiadh. | temporal transcription of phage phiadh was analysed during lytic reproduction. based on northern hybridizations the phage genome was divided into regions of early, middle and late transcription. eight groups of overlapping transcripts, probably originating from common precursors, were distinguished. early transcription of a 10.9 kb region adjacent to the lytic/lysogenic switch started within the first 10 min of infection and produced three groups of mrnas mostly related to dna replication. four ... | 2003 | 14523130 |
| identification, characterisation and specificity of a cell wall lytic enzyme from lactobacillus fermentum br11. | screening of a genomic library with an antiserum raised against whole lactobacillus fermentum br11 cells identified a clone expressing an immunoreactive 37-kda protein. analysis of the 3010-bp dna insert contained within the clone revealed four open reading frames (orfs). one orf encodes lysa, a 303 amino acid protein which has up to 35% identity with putative endolysins from prophages lj928 and lj965 from lactobacillus johnsonii and lp1 and lp2 from lactobacillus plantarum as well as with the e ... | 2004 | 15336396 |
| molecular properties of the two-component cell lysis system encoded by prophage phigay of lactobacillus gasseri jcm 1131t: cloning, sequencing, and expression in escherichia coli. | shotgun cloning of the lactobacillus gasseri jcm 1131t whole dna yielded two recombinant plasmids, p118gay1 and p118gay2, which directed cell lysis activity. sequencing analysis revealed that the two plasmids carried almost identical inserted genes in following orders (truncated genes, in parentheses): in p118gay1, (orf149)-orf92-holgay-lysgay-orf35-attl-(mnaagay1); in p118gay2, (orfxgay1)-orf169-orf149-orf92-holgay-lysgay-orf35-attp-(intgay). the lysgay-encoded protein (designated as lysgay, 33 ... | 2005 | 15808364 |
| codon utilization, dna landscaping and fractal analysis in bacteriophage phi(adh). | the bacteriophage phi(adh) has a low g+c content and encodes its protein products using a restricted number of the codons, which it could theoretically use. investigated were (i) the restricted codon usage by determining codon indices and codon distances for various genes and orfs, (ii) distribution of purines and pyrimidines on the two strands of the double-stranded genome and within all genes and orfs, and (iii) nucleotide positional bias within the genome. the genes and orfs can be clustered ... | 2005 | 16178514 |
| high-frequency plasmid transduction by lactobacillus gasseri bacteriophage phiadh. | the temperate bacteriophage phiadh mediates plasmid dna transduction in lactobacillus gasseri adh at frequencies in the range of 10 to 10 transductants per pfu. bglii-generated dna fragments from phage phiadh were cloned into the bcli site of the transducible plasmid vector pgk12 (4.4 kb). phage phiadh lysates induced from lactobacillus lysogens harboring pgk12 or the recombinant plasmids were used to transduce strain adh to chloramphenicol resistance. the transduction frequencies of recombinant ... | 1992 | 16348621 |
| plasmid transduction using bacteriophage phi(adh) for expression of cc chemokines by lactobacillus gasseri adh. | vaginal mucosal microfloras are typically dominated by gram-positive lactobacillus species, and colonization of vaginal mucosa by exogenous microbicide-secreting lactobacillus strains has been proposed as a means of enhancing this natural mucosal barrier against human immunodeficiency virus (hiv) infection. we asked whether an alternative strategy could be utilized whereby anti-hiv molecules are expressed within the cervicovaginal milieu by endogenous vaginal lactobacillus populations which have ... | 2010 | 20418431 |