| effects of fixation and postfixation treatments on volume of injured cells;. | the effects of fixation with glutaraldehyde (ga), formaldehyde (fa), glutaraldehyde-formaldehyde (ga-fa), flutaraldehyde-osmium tetroxide (ga-os04) and osmium tetroxide (oso4) on cel volume were studied in control, p-chloromercuribenzene sulfonic acid (pcmbs)-treated and hypotonically-treated ehrlich ascites tumor cells. among the variables investigated were concentration of the tixative agent, osmolity of the buffer, total osmolaity of the fixation solution, osmolaltity of the postfixation buff ... | 1975 | 805169 |
| dna-protein complex in circular dna from bacillus bacteriophage ga-1. | dna prepared from bacteriophage ga-1 contains circular dna molecules, which are converted to linear molecules by treatment with trypsin. | 1976 | 1271523 |
| glutaric aciduria type 1 an atypical presentation together with some observations upon treatment and the possible cause of cerebral damage. | this report describes an infant diagnosed aged twenty-five months as having glutaric aciduria type 1 (ga 1). initial presentation was with isolated macrocephaly at four months of age. severe hypertonia, and dystonia, within 24 hours of minor head injury occurred at nineteen months of age. serial cranial imaging showed subdural fluid collections, and increasing underlying cerebral atrophy, mainly frontal and temporal. confirmation of the clinical diagnosis required repeated blood and urine analys ... | 1992 | 1491752 |
| effects of antibodies to sperm surface fertilization antigen-1 on human sperm-zona pellucida interaction. | to investigate the effects of antibodies to well-defined sperm surface antigens (the fertilization antigen [fa-1] and germ-cell antigen [ga-1]) and nuclear antigen (protamine) on human sperm-zona interaction. | 1992 | 1601155 |
| mechanisms of fungal pathogenicity: correlation of virulence in vivo, susceptibility to killing by polymorphonuclear neutrophils in vitro, and neutrophil superoxide anion induction among blastomyces dermatitidis isolates. | seven blastomyces dermatitidis isolates varying in virulence for mice were compared for susceptibility to polymorphonuclear neutrophil (pmn) killing and the ability to induce superoxide anion (o2-) production by pmns in vitro. in vitro killing of six b. dermatitidis isolates by murine peripheral blood pmns or by pmns elicited from the peritoneal cavity by a local immune reaction (b. dermatitidis-immune mice given killed b. dermatitidis intraperitoneally 24 h earlier) inversely correlated with in ... | 1991 | 1649799 |
| characterization of proteins of chicken infectious anemia virus with monoclonal antibodies. | eight monoclonal antibodies (mabs) against chicken infectious anemia virus (ciav) were developed. these mabs identified three isolates adapted to grow in the marek's disease chicken cell line msb1 (cux-1, ga-1, and conn-b) and the chicken-propagated cia-1 isolate. all mabs stained msb1 in the same way with mostly perinuclear staining, although larger nuclear inclusions and cytoplasmic staining were also detected. none of the mabs neutralized cux-1. all mabs reacted in a direct enzyme-linked immu ... | 1991 | 1786015 |
| human spermatozoal fa-1 binds with zp3 of porcine zona pellucida. | experiments were carried out to study the binding of the major porcine zona pellucida glycoprotein zp3 (mr = 55,000) with human fa-1, a fertilization-related antigen isolated from human male germ cell plasma membranes and with ga-1, a germ cell antigen isolated from murine male germ cell plasma membranes. fa-1 showed a dose-dependent binding with zp3 in an enzyme-linked immunosorbent assay (elisa) and by the western blot procedure. fa-1 also bioneutralized the sperm ligand activity of zp3 in a b ... | 1991 | 1875328 |
| pulp response to collagen and glutaraldehyde in pulpotomized primary teeth of baboons. | this investigation assessed histologically the pulp tissue reaction to glutaraldehyde (ga) and to a commercial collagen preparation in pulpotomized primary teeth of baboons. one hundred and eighty-eight primary teeth were pulpotomized; in half of them inflammation was induced prior to the treatment. the teeth were divided into five groups: in three of them ga was used as a pulp dressing and applied for 1 min (group 1), 5 min (group 2), or mixed into the paste (group 3); collagen was used in grou ... | 1991 | 1909024 |
| [antitumor effect of direct intra-tumor administration of ok-432 on malignant glioma: basic and clinical observations]. | in this study, we tried to examine the efficacy of a cytotoxic factor which is induced by periodic, repeated local administration of ok-432 into the tumor cavity of malignant glioma patients. ok-432 was administered intratumorally via a tube to 4 malignant glioma patients on days 1, 3, 5, 7 and 12 in doses of 0.5 ke, 1 ke, 2 ke, and 3 ke, respectively. cerebrospinal fluid (csf) was collected several times during the 24-hour period beginning immediately after the administration on day 12. the csf ... | 1991 | 2038413 |
| photoregulated gene expression may involve ubiquitous dna binding proteins. | several promoter elements have previously been shown to influence the expression of the cab-e gene in nicotiana plumbaginifolia. here we demonstrate, by electrophoretic mobility shift and methylation interference assays, that a complex pattern of protein-dna interactions characterizes this promoter. among the multiple proteins identified, we focused on five different factors which either occupied important regulatory elements and/or were present in relatively large amounts in nuclear extracts. a ... | 1990 | 2209551 |
| infectious anemia caused by a parvovirus-like virus in georgia broilers. | pale chicks with necrotic dermatitis, small bursas of fabricius (bfs), small thymuses, pale bone marrow, and watery blood were suspected of having parvovirus-like virus- (pvlv) associated disease. histologic lesions included atrophy or hypoplasia of thymuses and bfs, and septic necrotizing clostridial dermatitis and hepatitis. clostridium perfringens was cultured from skin and liver. a pvlv was isolated in a marek's disease tumor cell line (mdcc-msb1) culture and was identified by physicochemica ... | 1989 | 2549935 |
| [mother and child stress parameters during cesarean section with general and peridural anesthesia]. | this study compared maternal and fetal stress responses during cesarean section in either general anesthesia (ga) or epidural anesthesia (ea). ten patients received ga with thiopental induction, intubation, and controlled ventilation with nitrous oxide and oxygen. after delivery, anesthesia was supplemented with fentanyl 0.2-0.3 mg. ten patients received ea via catheter, using bupivacaine 0.5%, and prilocaine or lidocaine 1%. maternal mean arterial pressure (map), hr, and plasma concentrations o ... | 1989 | 2554373 |
| the successful system in long-term cultivation of trypanosoma gambiense bloodstream forms. | rat astroglioma cell line (ga-1) was extremely useful for long-term in vitro cultivation of trypanosoma gambiense blood-stream forms. parasites could be continuously grown at 37 degrees c for more than 200 days in the culture system, consisted of hepes-buffered rpmi 1640 (ph 7.2, 300 milliosmole/kg) supplemented with 20% inactivated fetal calf serum in the presence of ga-1 cells. parasites cultured for more than 200 days still retained not only their virulence for mice but also their original an ... | 1986 | 3787303 |
| two forms of g(o) type g proteins: identification and distribution in various rat tissues and cloned cells. | a g(o) type g protein distinct from the major species of g(o) was recently isolated from bovine brain and designated g(o)*. the cdnas encoding two forms of mammalian g(o) alpha were also isolated and designated goa alpha and gob alpha. to recognize two forms of g(o) type g proteins, we raised antibodies in rabbits against two peptides with sequences found only in the respective proteins of murine goa alpha (sntyedaaayiqtqf) and gob alpha (teavahiqgqywsk). purified anti-goa alpha antibodies react ... | 1992 | 1573397 |
| cross-reaction of a monoclonal antibody with fibroblasts from marsupials of the macropodidae and tarsipedidae families. | a monoclonal antibody, ga-1, was prepared against an m. rufogriseus cell surface antigen on an m. rufogriseus (red necked wallaby)--mouse somatic cell hybrid. fibroblasts from a number of marsupial species were tested for reaction with ga-1, and only those from the macropodidae species and the sole member of the family tarsipedidae reacted, indicating that the tarsipedidae is possibly more closely related to the macropodidae than to any other group of marsupials examined. | 1985 | 4062701 |
| characterization of a glial associated antigen ga-1 by monoclonal antibody. | a glial antigen (ga-1) was identified by monoclonal antibodies (mab) raised against c6 rat glioma cells. mab-7d3 (igg2a kappa) revealed ga-1 as a single protein band with a rf value of 0.09 by the use of basic-page western blot. sds-page western blot and radioimmunoprecipitation (rip) further resolved ga-1 into two subunits with a molecular weight of 200 and 78 kda respectively. subcellular localization by immunocytochemical staining revealed its cytosolic presence with a punctate pattern perinu ... | 1992 | 1501776 |
| cell wall composition of two strains of blastomyces dermatitidis exhibiting differences in virulence for mice. | cell walls isolated from two strains of blastomyces dermatitidis were examined. whereas strain ga-1 was practically avirulent for mice, strain kl-1 produced death by 21 days in 50% of the mice inoculated. analyses of the trypsin-treated cell walls of the two strains revealed a higher chitin and protein content in strain kl-1, whereas a higher polysaccharide content was observed in the cell walls of strain ga-1. extraction of the walls with 1 n naoh revealed a threefold difference in the amount o ... | 1972 | 4636784 |
| a new allele of ga-1-r, the gametophytic gene of the fourth chromosome in maize. | | 1971 | 5163424 |
| idiotypic analysis of hybridoma antibodies to branched synthetic polymer (tyr, glu)-ala-lys: idiotypic relationship with antibodies to linear random polymer (glu60, ala30, tyr10). | the expression of three anti-gat idiotypes, cgat, gte, and ga-1, on 17 c57bl/10 and four c3h.sw hybridoma anti-(t,g)-a--l antibodies was analyzed. these hybridoma anti-(t,g)-a--l antibodies exhibited two patterns of fine antigen binding specificity. the majority of the hybridoma antibodies bound the (t,g)-a--l, gt, and gat polymers but not the ga polymer, and were designated as gt-reactive hybridoma antibodies. a minor population of hybridoma anti(t,g)-a--l antibodies bound to (t,g)-a--l but not ... | 1982 | 6172496 |
| isolation and characterization of periaxolemmal and axolemmal enriched membrane fractions from the rat central nervous system. | in this report, we describe the fractionation of crude axolemmal fractions from rat lower brainstem into subfractions enriched in markers for either periaxolemmal myelin or axolemma. these subfractions were isolated on density gradients as bands layering on 0.8m and 1.0m sucrose. both subfractions consisted of unilamellar vesicles. relative to myelin purified from the same starting material, the 0.8m subfraction was enriched in mag, cnpase, carbonic anhydrase and na+, k+ atpase but was extremely ... | 1992 | 1382138 |
| the use of growth and liver aspartate aminotransferase to assess the effect of source of non-essential nitrogen on pyridoxine depletion, repletion and requirements of chicks. | 1. two experiments were carried out to study the relationship between growth, liver aspartate aminotransferase (asp at) and dietary pyridoxine to determine the pyridoxine requirement of chicks fed on diets containing crystalline essential amino acids with glutamic acid (ga) or diammonium citrate (dahc) as the non-essential nitrogen source. 2. in one experiment purified diets containing isolated soy-protein with 0 or 3 mg pyridoxine/kg were used. the deficient chicks were significantly lighter, c ... | 1976 | 1245000 |
| idiotypic analysis of anti-gat antibodies. vi. identification and strain distribution of the ga-1 idiotype. | | 1979 | 91634 |
| provisional mapping of the gene for a cell surface marker, ga-1, in the red-necked wallaby macropus rufogriseus. | a series of m. rufogriseus-mouse somatic cell hybrids was constructed and analysed cytologically, enzymatically and immunologically. a monoclonal antibody, ga-1, was prepared against an m. rufogriseus cell surface antigen on an m. rufogriseus-mouse somatic cell hybrid. a gene determining the expression of this antigen was provisionally assigned to the long arm of the m. rufogriseus chromosome 3. the monoclonal antibody also reacted with an m. rufus (red kangaroo)-mouse somatic cell hybrid contai ... | 1985 | 3834888 |
| reduction of fertility in female rabbits and mice actively immunized with a germ cell antigen (ga-1) from the rabbit. | female rabbits and mice were actively immunized against germ cell antigen (ga-1) of 63 kda molecular mass isolated from rabbit sperm and testis. there was a significant (p less than 0.05) reduction of fertility in rabbits actively immunized with ga-1 as compared to controls, as seen by the percentage of 9-day implants/corpora lutea ratio (ga-1, 36.3%; controls, 85.7%). in mice, there was again a significant (p less than 0.01) reduction in fertility as seen by mean 7-9 day implants +/- s.d. per m ... | 1986 | 3806526 |
| structures of the o-linked oligosaccharides of the major cell surface sialoglycoprotein of mat-b1 and mat-c1 ascites sublines of the 13762 rat mammary adenocarcinoma. | structures of the principal o-glycosides from the major cell surface sialoglycoprotein (asgp-1) of the mat-b1 and mat-c1 ascites sublines of the 13762 rat mammary adenocarcinoma have been determined. oligosaccharitols were released by alkaline borohydride treatments of asgp-1 and purified by gel filtration, deae-sephadex ion exchange chromatography, and high performance liquid chromatography. on the basis of carbohydrate composition, methylation analysis, periodate oxidation, and exoglycosidase ... | 1984 | 6715325 |
| idiotypic analysis of anti-gat antibodies. vii. common idiotype on hybridoma antibodies to poly(glu60 ala40) | a single dba/2 mouse, immunized with l-glutamic acid60-l-alanine40 (ga), was used to produce hybridoma cell lines. seven hybridoma anti-ga antibodies were obtained for idiotypic analyses. two hybridoma anti-l-glutamic acid60-l-alanine30-l-tyrosine10 (gat) antibodies, preferentially reactive to ga, were studied in parallel. anti-idiotypic antisera to purified anti-gat and anti-ga serum antibodies and to hybridoma anti-ga antibodies were analyzed by idiotype binding and inhibition of idiotype bind ... | 1980 | 6774014 |
| sialylated glycolipid antigens on human leukemic cell lines. | many granulocyte-specific mouse monoclonal antibodies recognize the carbohydrate sequence 3-fucosyllactosamine, ga 1 beta 1-4[fuc alpha 1-3]glcnac, which occurs in cell-surface glycolipids and glycoproteins. in general, these antibodies bind to blast cells from most patients with acute myeloblastic leukemia, but not to those with acute lymphocytic leukemia. neuraminidase treatment, however, increases exposure of this antigen on both myeloid and lymphoid cells. in the present study, the glycolipi ... | 1986 | 3732420 |
| pathogen factors and host factors in murine pulmonary blastomycosis. | factors related to pathogenesis were studied in a murine model of pulmonary fungal infection with strains of blastomyces dermatitidis. strain 26199v was greater than or equal to 10(5)-fold more lethal in pulmonary challenge than strain ga-1. this relationship between the two strains also occurred after intraperitoneal challenge. serial studies with lung cultures and histology of sacrificed animals after pulmonary challenge with similar doses, and challenge studies in mice of differing maturity, ... | 1982 | 7121557 |
| autocrine regulation of glial proliferation and differentiation: the induction of cytodifferentiation of postmitotic normal glioblast by growth-promoting factor from astrocytoma cell. | a growth-promoting factor (gpf) from astrocytoma cells (ga-1) cultured in serum-free medium (n2) exerted on normal glioblasts proliferative and differentiation-promoting effects, which have been observed in glia maturation factor (gmf) stimulation. the serum-free conditioned media of ga-1 provoked dna synthesis of glioblasts, and subsequently elicited a morphological differentiation characterized by the extrusion of processes as well as biochemical changes including an increased cellular level o ... | 1986 | 3719342 |
| inhibition of proliferative growth in glioma cells by calmodulin antagonists. | the effects of several calmodulin antagonists, such as n-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide hydrochloride (w-7) and its dechlorinated structural analogue (w-5), on the growth and proliferation of cultured and transplanted glioma (ga-1, chemically induced from rat glioblasts) were evaluated. under culture conditions, the concentration of w-7 necessary to exert 50% inhibition of ga-1 glioma cell growth was 50 microm. however, w-5, with a lower binding affinity to calmodulin than w-7 ... | 1986 | 3712030 |
| immunochemical detection of gtp-binding protein in cephalopod photoreceptors by anti-peptide antibodies. | we prepared polyclonal antibodies (pab) against the following peptides: partial sequences of bovine transducin alpha subunit including the adp-ribosylation sites sensitive to cholera toxin (ctx) and pertussis toxin (ptx) and the n-terminus of drosophila gtp-binding protein q alpha (dgqn); pab ctx, pab ptx and pab dgqn, respectively. these antibodies were specific to the peptides used as antigen and no crossreactivity was observed. pab ctx and pab ptx reacted with bovine transducin alpha subunit ... | 1993 | 7764138 |
| in vitro pollen plant induction and embryoid clone establishment of panax ginseng. | in anther culture of panax ginseng, its callus formation showed a wide adaptability to culture media. large numbers of calli were induced on media exhibiting better effects of induction. supplements of 5 mg 2,4-d/1 and 1 mg kt/1 to the media proved to be much effective. regeneration of the whole plantlets from anther culture of panax ginseng is usually quite difficult. during the past three years, however, sixteen of the 100 medium formulae tested were proved to be suitable. the formulae of ms + ... | 1987 | 3445136 |
| anodic polarization behavior and microstructure of a gallium-based alloy. | a gallium-based alloy (ga) that was developed as a substitute for dental amalgam was investigated for anodic polarization behavior in deoxygenated ringers solution, 37 degrees c. the related microstructures were examined and microanalyses were conducted. four polarization tests were conducted by scanning from -300mv to +1,000 mv (vs. sce) at 2 mv/s. polarization of the first sample (ga-1) was stopped after the first anodic dissolution peak (-100 mv, 1.5-2.0 x 10(-3) a/cm2). the fourth sample (ga ... | 1993 | 7988754 |
| respiratory compliance of healthy newborn infants measured by endinspiratory airway occlusion technique in the first hours of life. | the compliance of the respiratory system (crs) has been measured by an airway occlusion technique in 78 healthy newborn infants [gestational age (ga) 36.2 +/- 2.9 weeks, range 28-41 weeks; birth weight (bw) 2.418 +/- 0.879 kg, range 0.830-4.350 kg; body height (bh) 45.2 +/- 4.4 cm, range 33-52 cm in the first 8 h after birth (206.2 +/- 100.8 min, range 45-480 min). the prediction equations were (crs, ml/cm h2o): crs = 0.087.ga -1.173 (r = 0.49, p less than 0.0001), crs = 0.372.bw +1.067 (r = 0.6 ... | 1988 | 3420305 |
| a comparison of the antihepatotoxic activity between glycyrrhizin and glycyrrhetinic acid. | a comparison of antihepatotoxic activities between glycyrrhizin (18 beta-gl) and its genuine aglycone, glycyrrhetinic acid (18 beta-ga), was carried out using in vivo and in vitro assay methods. the oral administration of 18 beta-ga at 1, 24, and 48 h before d-galactosamine (galn) treatment significantly reduced the increase of serum transaminase activities 24 h after galn treatment, whereas 18 beta-gl did not inhibit the increase of serum transaminase activities. the intraperitoneal administrat ... | 1994 | 8202565 |
| trypsin-stimulated transformation of trypanosoma brucei gambiense bloodstream forms to procyclic forms in vitro. | the effect of trypsin treatment on the transformation of monomorphic trypanosoma b. gambiense (wellcome strain) bloodstream forms to procyclic forms was studied in hepes-buffered rpmi 1640 medium supplemented with 20% inactivated fetal calf serum in the presence of ga-1 cells as feeder layers at 27 degrees c. in this system, 35%-40% of the bloodstream forms transformed to procyclic forms within 24 h, and over 95% of the trypanosomes changed into procyclic forms by day 3 after initiation of the c ... | 1988 | 3194362 |
| signals in the phi 29 dna-terminal protein template for the initiation of phage phi 29 dna replication. | the protein-free terminal fragments hindiii b and l, from the left and right ends of phi 29 dna, respectively, but not internal fragments of similar size, were active as templates in the formation of the p3-damp initiation complex in an in vitro system containing purified phi 29 terminal protein p3 and dna polymerase p2, although the activity was lower than that obtained with the phi 29 dna-p3 complex. these results indicate the existence of specific sequences at the ends of phi 29 dna that allo ... | 1986 | 3097958 |
| free energies for refolding of the common beta turn into the inverse-common beta turn: simulation of the role of d/l chirality. | quantitative estimates of the gibbs free-energy change (delta g) for refolding of one beta-turn conformation into another would assist rational protein design. for beta-turn models, we studied a chirally representative set of nine peptides of the form ch3co-l1-l2-nhch3, where loop residues l1 (i + 1) and l2 (i + 2) are achiral gly (g), l-ala (a), or d-ala (a). the stabilities of their common (type i) and inverse-common (type i') beta-turn conformers (ggi is the type-i gg conformer, etc.) were es ... | 1993 | 8356099 |
| s-100 protein in clonal astroglioma cells is released by adrenocorticotropic hormone and corticotropin-like intermediate-lobe peptide. | s-100 protein in clonal ga-1 and c6 rat glioma cell lines was released in serum-free medium supplemented with adrenocorticotropic hormone (acth). the induction of s-100 protein release by acth was dose-dependent, showing a half-maximal release at about 5 microm, and the s-100 protein concentration in the medium increased sharply within 3 min, but slightly during further incubation. the s-100 protein release was apparently accompanied by a decrease in the membrane-bound form of s-100 protein in t ... | 1987 | 2822856 |
| [a study of image processing condition of mammography using fcr (fuji computed radiography)]. | image processing conditions of mammography using fcr (fuji computed radiography) to detect mass shadow, marginal finding and microcalcification of breast cancer were examined. the subjects of the examination for mass shadow and marginal finding were 25 cases out of 55 breast cancers operated from december 1986 to august 1988. three surgeons and two radiologists evaluated processing conditions. the optimum conditions of spatial frequency enhancement were rn(1 or 3) rt(p) re(1.0) for mass shadow a ... | 1989 | 2798048 |
| [effectiveness of cycloplatam in the free form and included in liposomes during metastasis of ga-1 tumors in a/he mice]. | the 6-10-fold freezing and thawing of routine multilamellar phospholipid vesicles in cycloplatam solution give rise to liposomes which entrap in their aqueous phase up to 10 mg of the drug per 100 mg of lipids. when injected intravenously to mice with ha-1 tumour metastases in the liver, liposome-encapsulated cycloplatam increased their survival rate by 112-123%, whereas free cycloplatam--only by 39-61% as compared to the control. the intramuscular transplants of the tumour were affected similar ... | 1989 | 2791961 |
| immunogenicity and relative attenuation of different vaccinia-rabies virus recombinants. | immunogenicity and relative attenuation were examined for the following tian tan strain vaccinia-rabies recombinant viruses: 1) ngc-1, which coexpresses the glycoprotein (g) and nucleocapsid protein (n) of the rabies virus challenge virus standard (cvs) strain; 2) nc-1, which expresses the cvs n; 3) gc-2, gc-3, gc-4, and gc-5, which express cvs g via promoters from different vaccinia strains or from different vaccinia genome loci; 4) ga-1, which expresses the g of rabies virus strain ag; and 5) ... | 1996 | 8712923 |
| structural and neurobehavioral delay in postnatal brain development of preterm infants. | postnatal brain development of healthy prematurely born infants was assessed to study possible influence of premature birth and early extrauterine environment on structural, biochemical, and functional brain development. myelination and differentiation of gray and white matter were studied by in vivo magnetic resonance (mr) imaging (mri), changes in cerebral metabolism by 1hmr spectroscopy (mrs), and changes in early human neurobehavior by the assessment of preterm infant's behavior (apib). the ... | 1996 | 8726248 |
| [effectiveness of free and liposomal forms of platinum and anthracycline antibiotics for hepatic metastasis of ga-1 and p-388 tumors in mice]. | liposome-entrapped platinum and anthracycline antibiotics showed similar advantages over their free forms in both experimental runs in being less toxic and more effective as a means of metastasis control. since individual tumors differ in their sensitivity to different drugs, it might be suggested that liposomal forms of platinum coordination complexes may contribute to the hepatic metastasis drugs stockpile. | 1996 | 8801744 |
| the plant g box promoter sequence activates transcription in saccharomyces cerevisiae and is bound in vitro by a yeast activity similar to gbf, the plant g box binding factor. | g box and i box sequences of the arabidopsis thaliana ribulose-bisphosphate-1,5-carboxylase small subunit (rbcs) promoter are required for expression mediated by the arabidopsis rbcs-1a promoter in transgenic tobacco plants and are bound in vitro by factors from plant nuclear extracts termed gbf and ga-1, respectively. we show here that a -390 to -60 rbcs-1a promoter fragment containing the g box and two i boxes activates transcription from a truncated iso-1-cytochrome c (cyc1) gene promoter in ... | 1990 | 2161333 |
| regional hemodynamic effects produced following application of l-glutamic acid to the ventral medullary surface of the cat. | the neuronal stimulant l-glutamic acid (ga) was applied to the ventral medullary surface and diastolic arterial blood pressure (dbp), heart rate (hr), renal and femoral arterial blood flow were measured in the chloralose-anesthetized cat. ga (1.0 m) increased dap by 27 +/- 6.6 mmhg (p less than 0.05, n = 6), but heart rate was not affected (p greater than 0.05). renal arterial resistance (rr) increased by 15 +/- 5.8% (p less than 0.05) and femoral resistance (fr) increased by 46 +/- 10% (p less ... | 1990 | 1968242 |
| antitumor activity on murine tumors of a novel antitumor benzoylphenylurea derivative, ho-221. | a novel antitumor compound, n-[4-(5-bromo-2-pyrimidinyloxy)-3-chlorophenyl]-n'-(2-nitrobenzoyl ) urea (ho-221) was evaluated for its antitumor activity in experimental tumor models. ho-221 preparation was given orally to tumor-bearing animals. the compound exhibited significant effects against various tumors such as p388 and l1210 leukemias; m5076 reticulum-cell sarcoma; colon 38 carcinoma; human xenografts mx-1, lx-1, ga-1, and co-1; lewis lung carcinoma; sarcoma 180; and walker 256 carcinosarc ... | 1991 | 1914078 |
| detection of g proteins in bovine brain clathrin coated vesicles with common alpha and beta subunits antibodies. | clathrin coated vesicles (cv) from bovine brain were analyzed via western blots for the presence of the alpha and beta subunits of guanine nucleotide regulatory proteins. the results with the common alpha antibody ga/1 revealed the presence of apparently undissociated g-protein subunits migrating at approximately 80 kda. the predominant band was of about 39-41 kda, with minor labeling observed in the 41-52 kda range. western blot analysis for g beta subunit(s) revealed the presence of a single b ... | 1991 | 1908919 |
| translocation and induction of alpha b crystallin by heat shock in rat glioma (ga-1) cells. | response to heat shock of alpha b crystallin expressed in rat astrocytoma ga-1 cells was analysed quantitatively using an immunoassay method. ga-1 cells contained a considerable amount of alpha b crystallin at growing phase. when the growing cells were heated at 45 degrees c, concentrations of alpha b crystallin in cell extracts were decreased to less than one-fifth of the original level within 15 min, with an increase in the insoluble fraction which was detected by immunoblotting. the low level ... | 1992 | 1734885 |
| lack of association between helicobacter pylori and gastric atrophy or intestinal metaplasia in elderly patients. | helicobacter pylori (hp) is associated with gastritis and peptic ulcers. it may also induce gastric atrophy (ga) and intestinal metaplasia (im), and these changes may be the precursors of gastric carcinoma. the aim of this study was to determine if ga or im is associated with hp infection in elderly patients. consecutive patients admitted for gastroscopy were recruited. multiple biopsies were taken for histology and rapid urease (clo) tests along with igg enzyme-linked immunosorbent assay antibo ... | 1996 | 9138979 |
| refined fetal abdominal growth assessment in normal pregnancy: part i. abdominal anteroposterior diameter. | the assessment of the adequacy of fetal growth by parameters other than the abdominal anteroposterior diameter (aapd) of the fetus has been studied extensively. however the designs of these studies and the statistical methods used appears to deserve some criticism, based on present knowledge. noncross-sectional cases selection, uncertainty of the fetal normality and inadequacy in statistical method, mostly ignored the changing property of each standard deviation (sd) of each gestational age (ga) ... | 1997 | 9198291 |
| mineralocorticoid excess, dietary sodium, and myocardial fibrosis. | unlike the non-renin-dependent hypertension associated with infrarenal aorta banding, an abnormal accumulation of fibrillar collagen occurs within the adventitia of intramural coronary arteries and neighboring interstitial space of the left and right ventricles in arterial hypertension associated with primary or secondary hyperaldosteronism. based on these findings it was suggested that this interstitial and perivascular fibrosis was mediated by mineralocorticoid excess (i.e., elevated plasma al ... | 1992 | 1453111 |
| expression cloning and characterization of a renal organic anion transporter from winter flounder. | the cdna coding for a renal p-aminohippurate (pah) transporter from winter flounder (pseudopleuronectes americanus), designated froat, was cloned by functional expression in xenopus laevis oocytes. froat is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter roat1/oat1 and the organic cation transporters oct1 and oct2. in oocytes, froat mediated probenecid-sensitive pah uptake, with a km for pah of about 20 microm, and inhi ... | 1997 | 9409735 |
| glutaric aciduria type 1: an underdiagnosed cause of encephalopathy and dystonia-dyskinesia syndrome in children. | two cases of glutaric aciduria type 1 (ga 1) are presented. ga 1 is probably underdiagnosed and misdiagnosed, and may explain a proportion of cases of extrapyramidal and 'postencephalitic' cerebral palsy. most cases of ga 1 present with a severe dystonic-dyskinetic syndrome following an acute encephalopathy. asymptomatic cases have also been described, complicating genetic counselling and prenatal diagnosis. we raise awareness of ga 1 and stress that if clinically suspected, immediate institutio ... | 1998 | 9633984 |
| characterization of a gi-protein from trypanosoma cruzi epimastigote membranes. | a guanosine 5'-[gamma-[35s]thio]triphosphate-binding activity was detergent-extracted from trypanosoma cruzi membranes. this binding activity was co-eluted from gel-filtration columns with a factor which, in a heterologous reconstitution system, blocks glucagon stimulation of adenylate cyclase activity in liver membranes. adp-ribosylation of these membranes by pertussis toxin eliminated this blocking capacity. incubation of t. cruzi membranes with activated pertussis toxin and [adenylate-32p]nad ... | 1992 | 1445203 |
| nucleotide sequence analysis of dna replication origins of the small bacillus bacteriophages: evolutionary relationships. | the ends of the small bacillus phage genomes serve as origins and termini of their dna replication. we have determined nucleotide sequences at the termini of four different phage dnas and compared them with those of phi 29 dna which has been described previously. a high degree of homology was found at the extreme ends of dnas from phi 29, phi 15 (group a), m2y and nf (group b). 17 bp at the far left of the dnas are identical. a highly conserved dodecanucleotide sequence, ccatttccccat, was also f ... | 1985 | 3932129 |
| interrelationships among distinct idiotypic specificities. | the fine idiotypic properties of a hybridoma (h51.85.2) anti-gat antibody which lacks both cgat and ga-1 idiotypes are described. we identified another idiotype, termed ga-2 idiotype, on h51.85.2 hybridoma antibody. the ga-2 idiotype is present in all mouse strains tested and is induced by ga-related antigenic determinants. an interesting relationship between ga-1 and ga-2 idiotype on hybridoma anti-ga antibodies was observed. all ga-1+ hybridomas also express ga-2 idiotype. in contrast, ga-2+ h ... | 1984 | 6712164 |
| terminal proteins and short inverted terminal repeats of the small bacillus bacteriophage genomes. | the genome of bacillus phage phi 29 contains covalently linked protein at both ends. these dna terminal proteins are essential for phi 29 dna replication. we have isolated phi 29 terminal protein from each end separately and compared their two-dimensional peptide maps. our results showed the two proteins to be identical. the dnas of four phages examined (phi 15, nf, m2y, and ga-1) also contain protein at both ends of the dna molecules. the chymotryptic peptide maps of these dna terminal proteins ... | 1981 | 6941313 |
| locoregional versus general anesthesia in carotid surgery: is there an impact on perioperative myocardial ischemia? results of a prospective monocentric randomized trial. | the incidence of cardiac morbidity and mortality in patients who undergo carotid surgery ranges from 0.7% to 7.1%, but it still represents almost 50% of all perioperative complications. because no data are available in literature about the impact of the anesthetic technique on such complications, a prospective randomized monocentric study was undertaken to evaluate the role of local anesthesia (la) and general anesthesia (ga) on cardiac outcome. | 1999 | 10394163 |
| gh3 cell-specific expression of kv1.5 gene. regulation by a silencer containing a dinucleotide repetitive element. | a silencer element (kv1.5 repressor element; kre) was characterized by deletion analyses in the promoter of kv1.5, a voltage-gated potassium channel. the silencer element selectively decreases expression of kv1.5- and thymidine kinase-chloramphenicol acetyl-transferase reporter gene constructs in cell lines that do not express kv1.5 polypeptide. it contains a dinucleotide repetitive element (poly(gt)19(ga)1(ca)15(ga)16), and self-associates spontaneously in vitro to form complexes with slow elec ... | 1995 | 7499248 |
| ganglioside gm(1a) on the cell surface is involved in the infection by human rotavirus kun and mo strains. | rotavirus is the most common cause of severe gastroenteritis in infants and children worldwide. the cell attachment of most animal rotaviruses, which belong to the neuraminidase-sensitive strains, requires sialic acid residues on the host cell membranes. on the other hand, most human rotaviruses are classified as neuraminidase-insensitive strains. the involvement of gangliosides on the host cell surface in human rotavirus infection was investigated by immunostaining analysis of target cells, and ... | 1999 | 10502675 |
| the slender gene of pea encodes a gibberellin 2-oxidase. | the amount of active gibberellin (ga) in plant tissues is determined in part by its rate of catabolism through oxidation at c-2. in pea (pisum sativum l.) seeds, ga 2-oxidation is controlled by the sln (slender) gene, a mutation of which produces seedlings characterized by a slender or hyper-elongated phenotype. we cloned a ga 2-oxidase cdna from immature pea seeds by screening an expression library for enzyme activity. the clone contained a full-length open reading frame encoding a protein of 3 ... | 1999 | 10557225 |
| g-proteins in rat blood vessels--i. identification. | 1. the purpose of this investigation was to identify various types of conventional, low and high molecular weight g-proteins in purified membranes of rat aorta and mesenteric artery. 2. in both blood vessels, immunoblotting of g-proteins using as/7 antibody specific for gi-1/2, ec/2 antibody specific for gi-3 and rm/1 antibody specific for gs-type conventional g-proteins demonstrated the presence of m(r) 28-43 kda, m(r) 41 and 48 kda, and m(r) 36-46 kda polypeptides, respectively. immunoblotting ... | 1995 | 7713368 |
| [detectability of intra-osseous lesions of jaws with computed panoramic radiography]. | the purpose of this study is to examine the diagnostic accuracy in the detection of jaw lesions with computed panoramic radiography and to find optimal parameters for every diagnostic purpose. a phantom of an artificial mandibular bone was devised, and radiolucent and radiopaque jaw lesions were simulated by a wedge-shaped cavity. panoramic radiographies were taken by varying the position of the phantom and the parameters of computed radiography (cr). the results were as follows: 1. detectabilit ... | 1994 | 7930821 |
| intravenous cannulation: a different approach. | this article presents a different method of venous cannulation. it has been used successfully to teach dental residents working on pediatric and developmentally disabled patients. the technique includes using a syringe of 1% lidocaine hydrochloride attached to a 25-ga needle to produce a skin wheal by "jet injection." this is accomplished by placing the bevel of the needle downward and forcing the syringe in a downward and backward direction, with pressure being continuously exerted on the plung ... | 1993 | 8185086 |
| chronic subdural hematoma, as an initial manifestation of glutaric aciduria type-1. | a 10-month-old male with glutaric aciduria type-1 (ga-1) is reported. this patient showed frequent partial motor seizures, irritability, and involuntary movements, including oral dyskinesia at the age of 3 months. on admission, magnetic resonance (mr) scanning revealed a chronic subdural hematoma and widening of the bilateral insular cisterns. urine organic acid analysis showed marked excretion of glutaric acid, 3-hydroxy glutaric acid and glutaconic acid, suggesting ga-1. removal of the subdura ... | 1993 | 8214331 |
| the gene pat-2, which induces natural parthenocarpy, alters the gibberellin content in unpollinated tomato ovaries. | we investigated the role of gibberellins (gas) in the effect of pat-2, a recessive mutation that induces facultative parthenocarpic fruit development in tomato (lycopersicon esculentum mill.) using near-isogenic lines with two different genetic backgrounds. unpollinated wild-type madrigal (ma/wt) and cuarenteno (cu/wt) ovaries degenerated, but ga(3) application induced parthenocarpic fruit growth. on the contrary, parthenocarpic growth of ma/pat-2 and cu/pat-2 fruits, which occurs in the absence ... | 2000 | 10677440 |
| identification of gq as one of the g-proteins which copurify with human platelet thromboxane a2/prostaglandin h2 receptors. | the present study employed ligand affinity and immunoaffinity chromatography to isolate human platelet thromboxane a2/prostaglandin h2 (txa2/pgh2) receptor-coupled g-proteins. purification of txa2/pgh2 receptors by ligand (sq31,491)-affinity chromatography resulted in the elution of receptor binding and gtpase activity in the same fraction. gtpase activity of this fraction was enriched (6-fold) relative to solubilized platelet membranes, was stimulated (65%) by 500 nm u46619, and was blocked (74 ... | 1993 | 8245033 |
| gibberellins and subapical cell divisions in relation to bud set and bud break in salix pentandra. | in young plants of salix pentandra, a temperate zone deciduous woody species, elongation growth ceases and a terminal bud is formed at day lengths shorter than a critical length. this is the first step in dormancy development, making survival under harsh winter conditions possible. early studies strongly indicate that gibberellin is involved in the photoperiodic control of bud set and bud break. ga(1) action was studied by application under short days to plants where cessation of shoot elongatio ... | 1999 | 10688705 |
| [development of brain atrophy, therapy and therapy monitoring in glutaric aciduria type i (glutaryl-coa dehydrogenase deficiency)]. | serial trans-fontanellar sonographic examination in a patient with glutaric aciduria type i (ga i) demonstrated that the typical frontotemporal cerebral atrophy developed postnatally within three months paralleling the onset of dystonic symptoms. pathogenesis of the accompanying macrocephaly remains unclear and can form a diagnostic pitfall. diet low in lysine and tryptophan led to a dramatic fall in urinary glutaric acid (ga) excretion but as in other patients with ga i did not substantially in ... | 1993 | 8445849 |
| protease-sensitive transfection of bacillus subtilis with bacteriophage ga-1 dna: a probable case of heterologous transfection. | the host bacterium of bacteriophage ga-1, bacillus sp. g1r, was compared with respect to its taxonomic relationship to bacillus subtilis, b. licheniformis, and b. pumilis. the physiological-biochemical properties of bacillus sp. g1r are equal to those of b. licheniformis, but the thermal denaturation midpoint of g1r dna differs by 3 c and the buoyant density by 0.005 g/cm(3) from that of b. licheniformis. transformation with g1r donor dna was neither observed in b. licheniformis nor in b. subtil ... | 1974 | 4207247 |
| gibberellins and seed development in maize. i. evidence that gibberellin/abscisic acid balance governs germination versus maturation pathways. | abscisic acid (aba) is required for the regulation of seed maturation in maize (zea mays l.). mutants blocked in aba synthesis (such as viviparous-5) do not mature to quiescent, desiccation-tolerant seeds, but germinate on the ear midway through kernel development. because gibberellins (ga) and aba act antagonistically in many aspects of plant development, we hypothesized that aba antagonizes a positive ga signal for precocious germination in maize. in these experiments, we show that a ga defici ... | 2000 | 10759503 |
| use of synthetic gramicidins in the determination of channel structure and mechanism. | the syntheses of (1-13c) trp9 gramicidin a (ga), (1-13c) trp11 ga, (1-13c) trp13 ga, (1-13c) trp15 ga, and d . leu2 ga are verified by means of high performance liquid chromatography, carbon-13 nuclear magnetic resonance, circular dichroism and characterization of transport properties. the use of these and other synthetic gramicidins is discussed in terms of determining ion binding sites within the channel, helix sense of the channel, the basis of monovalent vs divalent cation selectivity, and m ... | 1983 | 6186622 |
| repression of shoot growth, a bzip transcriptional activator, regulates cell elongation by controlling the level of gibberellins. | cell expansion, a developmental process regulated by both endogenous programs and environmental stimuli, is critically important for plant growth. here, we report the isolation and characterization of rsg (for repression of shoot growth), a transcriptional activator with a basic leucine zipper (bzip) domain. to examine the role of rsg in plant development, we generated transgenic tobacco plants expressing a dominant-negative form of rsg, which repressed the activity of full-length rsg. in transg ... | 2000 | 10852936 |
| structural bases for public idiotypic specificities of monoclonal antibodies directed against poly(glu60ala30tyr10) and poly(glu60ala40) random copolymers. | nh2-terminal amino acid sequences of heavy and light chains of seven poly(glu60ala30tyr10) (gat) specific hybridoma products derived from dba/2 and (dba/2 x balb/c)f1 hybrid mice and those of balb/b polyclonal antibodies have been determined over the first 40 residues. comparison of these sequences with those of nine other gat or poly(glu60ala40) (ga) specific hybridoma products previously reported allowed the following conclusions. (i) sequences of hybridoma h and l chains are present in the po ... | 1983 | 6190167 |
| template requirements for initiation of phage phi 29 dna replication in vitro. | the template requirements for the formation of the phi 29 protein p3-damp initiation complex in vitro have been studied. the initiation reaction requires the parental protein p3 but not an intact dna molecule. protein p3-containing fragments from the left- or right-hand dna ends were active as template for formation of the initiation complex provided they had a minimal size: a 26-base-pair-long fragment was active whereas a 10-base-pair-long one was essentially inactive. however, the activity of ... | 1984 | 6320176 |
| the absence of differentiation-promoting response of astroglioma cells to glia maturation factor. | the effects of glia maturation factor (gmf) on cell proliferation and differentiation were investigated with 3 astroglioma cells (ge-12, c6, and ga-1), schwannoma-like cells (354a), and mixed glioma cells (lrm-55). in the exponentially growing phase the growth rates of all glioma cells were enhanced by gmf regardless of the presence or absence of serum, but the factor failed to make the saturation density surpass the control level observed in the medium without gmf even in the chemically defined ... | 1984 | 6329449 |
| molecular phylogeny of phi29-like phages and their evolutionary relatedness to other protein-primed replicating phages and other phages hosted by gram-positive bacteria. | the phi29-like phage genus of podoviridae family contains phages b103, bs32, ga-1, m2, nf, phi15, phi29, and pza that all infect bacillus subtilis. they have very similar morphology and their genomes consist of linear double-stranded dna of approximately 20 kb. the nucleotide sequences of individual genomes or their parts determined thus far show that these phages evolved from a common ancestor. a terminal protein (tp) that is covalently bound to the dna 5'-end primes dna replication of these ph ... | 1999 | 9929388 |
| purification and preliminary characterization of a cardiac kv1.5 repressor element binding factor. | we have previously demonstrated that the cell-specific expression of kv1.5 promoter is regulated by a silencer (kv1.5 repressor element; kre) containing a dinucleotide-repetitive element, (gt)19(ga)1(ca) 15(ga)16. electromobility gel shift assays (emsas) of kre with gh3 nuclear extracts detected a unique dna-protein complex, which was not detectable in chinese hamster ovary or cos-7 cells. we further delineated kre and determined that a 52-bp fragment that contained a (gt)10(ga)1(ca)10 dinucleot ... | 1999 | 10222341 |
| expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice. | a major catabolic pathway for gibberellin (ga) is initiated by 2beta-hydroxylation, a reaction catalyzed by ga 2-oxidase. we have isolated and characterized a cdna, designated oryza sativa ga 2-oxidase 1 (osga2ox1) from rice (oryza sativa l. cv nipponbare) that encodes a ga 2-oxidase. the encoded protein, produced by heterologous expression in escherichia coli, converted ga(1), ga(4), ga(9), ga(20), and ga(44) to the corresponding 2beta-hydroxylated products ga(8), ga(34), ga(51), ga(29), and ga ... | 2001 | 11244129 |
| new class of magnetoresistance oscillations: interaction of a two-dimensional electron gas with leaky interface phonons. | we report on a new class of magnetoresistance oscillations observed in a high-mobility two-dimensional electron gas (2deg) in gaas-al(x)ga(1--x)as heterostructures. appearing in a weak magnetic field ( b < 0.3 t) and only in a narrow temperature range ( 2 k < t < 9 k), these oscillations are periodic in 1/b with a frequency proportional to the electron fermi wave vector, k(f). we interpret the effect as a magnetophonon resonance of the 2deg with leaky interface-acoustic phonon modes carrying a w ... | 2001 | 11328036 |
| phi29 family of phages. | continuous research spanning more than three decades has made the bacillus bacteriophage phi29 a paradigm for several molecular mechanisms of general biological processes, such as dna replication, regulation of transcription, phage morphogenesis, and phage dna packaging. the genome of bacteriophage phi29 consists of a linear double-stranded dna (dsdna), which has a terminal protein (tp) covalently linked to its 5' ends. initiation of dna replication, carried out by a protein-primed mechanism, ha ... | 2001 | 11381102 |
| potentiation of antitumor effects of cisplatin by tumor necrosis factor-beta. | recombinant tumor necrosis factor-beta potentiated the inhibitory effect of cisplatin on intramuscularly transplanted ga-1 tumor and liver metastasis in mice. the antitumor effect was related to cytotoxic and cytostatic activities of the test preparations rather than to initiation of apoptosis. | 2001 | 11391402 |
| the switch from early to late transcription in phage ga-1: characterization of the regulatory protein p4g. | the transcription program of the bacillus phage ga-1, a distant relative of phage phi29, has been studied. transcription of the ga-1 genome occurred in two stages, early and late. early genes were expressed from two promoters equivalent to the phi29 a2b and a2c promoters, whereas late transcription started at a site equivalent to the phi29 late a3 promoter. the activity of the ga-1 early a2b and a2c promoters diminished 10 minutes after infection, a time at which expression of the late promoter ... | 1999 | 10438592 |
| cloning and functional analysis of two gibberellin 3 beta -hydroxylase genes that are differently expressed during the growth of rice. | we have cloned two gibberellin (ga) 3 beta-hydroxylase genes, osga3ox1 and osga3ox2, from rice by screening a genomic library with a dna fragment obtained by pcr using degenerate primers. we have used full-scan gc-ms and kovats retention indices to show function for the two encoded recombinant fusion proteins. both proteins show 3 beta-hydroxylase activity for the steps ga(20) to ga(1), ga(5) to ga(3), ga(44) to ga(38), and ga(9) to ga(4). in addition, indirect evidence suggests that the osga3ox ... | 2001 | 11438692 |
| production of dwarf lettuce by overexpressing a pumpkin gibberellin 20-oxidase gene. | we investigated the effect of overexpressing a pumpkin gibberellin (ga) 20-oxidase gene encoding an enzyme that forms predominantly biologically inactive products on ga biosynthesis and plant morphology in transgenic lettuce (lactuca sativa cv vanguard) plants. lettuce was transformed with the pumpkin ga 20-oxidase gene downstream of a strong constitutive promoter cassette (el2-35s-omega). the transgenic plants in which the pumpkin gene was detected by polymerase chain reaction were dwarfed in t ... | 2001 | 11457947 |
| regulation of gibberellin 20-oxidase and gibberellin 3beta-hydroxylase transcript accumulation during de-etiolation of pea seedlings. | gibberellin (ga) 20-oxidase (ga 20-ox) and ga 3beta-hydroxylase (ga 3beta-hy) are enzymes that catalyze the late steps in the formation of active gas, and are potential control points in the regulation of ga biosynthesis by light. we have investigated the photoregulation of the ga 20-ox and ga 3beta-hy transcript levels in pea (pisum sativum l.). the ga 20-ox transcript level was higher in light-grown seedlings than in etiolated seedlings, whereas ga 3beta-hy mrna accumulation was higher in etio ... | 1999 | 10557226 |
| development of an in vivo assay to identify structural determinants in murine leukemia virus reverse transcriptase important for fidelity. | error-prone dna synthesis by retroviral reverse transcriptases (rts) is a major contributor to variation in retroviral populations. structural features of retroviral rts that are important for accuracy of dna synthesis in vivo are not known. to identify structural elements of murine leukemia virus (mlv) rt important for fidelity in vivo, we developed a d17-based encapsidating cell line (angie p) which is designed to express the amphotropic mlv envelope. angie p also contains an mlv-based retrovi ... | 2000 | 10590119 |
| the effect of gibberellins on flowering in roses. | the gibberellins a(1), a(3), a(5), a(8), a(19), a(20), and a(29) were identified in vegetative shoot tips of rosa canina by comparing their mass spectra and kovats retention indices with those of standards. most wild roses have a short flowering season of 2-4 weeks in spring, whereas most modern cultivars flower recurrently. 'félicité et perpétue' is a short-season hybrid from a cross between a wild rose and a recurrent-flowering rose, whereas its sport, 'little white pet,' flowers recurrently. ... | 1999 | 10594246 |
| hormonal changes in the grains of rice subjected to water stress during grain filling. | lodging-resistant rice (oryza sativa) cultivars usually show slow grain filling when nitrogen is applied in large amounts. this study investigated the possibility that a hormonal change may mediate the effect of water deficit that enhances whole plant senescence and speeds up grain filling. two rice cultivars showing high lodging resistance and slow grain filling were field grown and applied with either normal or high amount nitrogen (hn) at heading. well-watered and water-stressed (ws) treatmen ... | 2001 | 11553759 |
| long-day induction of flowering in lolium temulentum involves sequential increases in specific gibberellins at the shoot apex. | one challenge for plant biology has been to identify floral stimuli at the shoot apex. using sensitive and specific gas chromatography-mass spectrometry techniques, we have followed changes in gibberellins (gas) at the shoot apex during long day (ld)-regulated induction of flowering in the grass lolium temulentum. two separate roles of gas in flowering are indicated. first, within 8 h of an inductive ld, i.e. at the time of floral evocation, the ga(5) content of the shoot apex doubled to about 1 ... | 2001 | 11598236 |
| zener model description of ferromagnetism in zinc-blende magnetic semiconductors | ferromagnetism in manganese compound semiconductors not only opens prospects for tailoring magnetic and spin-related phenomena in semiconductors with a precision specific to iii-v compounds but also addresses a question about the origin of the magnetic interactions that lead to a curie temperature (t(c)) as high as 110 k for a manganese concentration of just 5%. zener's model of ferromagnetism, originally proposed for transition metals in 1950, can explain t(c) of ga(1-)(x)mn(x)as and that of it ... | 2000 | 10669409 |
| microalbuminuria in niddm is caused by increased excretion of unmodified albumin. | after an intravenous infusion of l-arginine to inhibit tubular reabsorption of albumin, glomerular clearance, renal clearance, and tubular reabsorption of unmodified albumin (uma) and glycated albumin (ga) were determined in 72 patients with niddm without (niddm-i; n = 47) or with microalbuminuria (niddm-ii; n = 25) and in 24 healthy control subjects. samples of serum albumin and dialyzed urine obtained 60 min before and during l-arginine infusion were applied to an affinity column to separate g ... | 1996 | 8635645 |
| evidence for heterotrimeric gtp-binding proteins in toxoplasma gondii. | toxoplasma gondii, an intracellular protozoan parasite, resides within a host-derived vacuole that is rapidly modified by a parasite-secreted membranous tubular network. in this study we investigated the involvement of heterotrimeric g proteins in the secretory pathway of t. gondii. aluminum fluoride (alfn), a specific activator of heterotrimeric g proteins, induced secretion from isolated tachyzoites of t. gondii in vitro, as seen by light optics and electron microscopy. in western blot analyse ... | 1996 | 8640189 |
| ontogeny of apical membrane ion conductances and channel expression in cortical collecting duct cells. | ontogeny of apical-basolateral epithelial cell polarity was studied by a comparison of embryonic ureteric bud (ub) and mature cortical collecting duct (ccd) primary cultures. patch-clamp techniques were applied to identify apical ion channels and to measure specific conductance (in ns/10 pf) of the apical membrane (ga) and of the whole cell (gw). in ub, gw (1.3 +/- 0.17; n = 32) and ga (1.4 +/- 0.15; n = 9) were not different, whereas they differed greatly in ccd (gw: 0.79 +/- 0.07, n = 35; ga: ... | 1996 | 8853433 |
| subdural hemorrhage as an initial sign of glutaric aciduria type 1: a diagnostic pitfall. | the case of a 9-month-old girl with glutaric aciduria type 1 (ga 1) is reported. on initial presentation at 6 months of age, the patient demonstrated bilateral subdural hemorrhages and widening of the basal cisterns. after neurosurgical intervention the subdural effusions regressed; their etiology remained unclear. at the age of 9 months the patient presented again because of progressive loss of psychomotor abilities and a dystonic movement disorder. cerebral mri revealed regressive subdural hem ... | 1996 | 8929376 |
| gamyb-like genes, flowering, and gibberellin signaling in arabidopsis. | we have identified three arabidopsis genes with gamyb-like activity, atmyb33, atmyb65, and atmyb101, which can substitute for barley (hordeum vulgare) gamyb in transactivating the barley alpha-amylase promoter. we have investigated the relationships between gibberellins (gas), these gamyb-like genes, and petiole elongation and flowering of arabidopsis. within 1 to 2 d of transferring plants from short- to long-day photoperiods, growth rate and erectness of petioles increased, and there were morp ... | 2001 | 11743113 |
| activation of replication origins in phi29-related phages requires the recognition of initiation proteins to specific nucleoprotein complexes. | protein p6 of bacillus subtilis phage phi29 activates the initiation of viral dna replication by forming a multimeric nucleoprotein complex at the origins of replication, located at both ends of the linear genome. this activation requires a precise positioning of the protein p6 array with respect to the initiation site. to investigate this activation mechanism, we have purified the phi29 protein p6 counterparts from the related phages nf and ga-1 and analyzed the formation of complexes with dna. ... | 1996 | 8940089 |
| functional characterization of the genes coding for the terminal protein and dna polymerase from bacteriophage ga-1. evidence for a sliding-back mechanism during protein-primed ga-1 dna replication. | we have determined the nucleotide sequence of 2676 bp at the left part of the linear genome of bacillus subtilis bacteriophage ga-1. computer analysis revealed that this fragment contains two open reading frames (orfs), orf1 and orf2, which contain 265 and 578 codons, respectively. data base search revealed that orf1 and orf2 might encode proteins similar to the terminal protein (tp) and dna polymerase, respectively, of bacteriophage phi29. by using extracts of b. subtilis infected with ga-1, we ... | 1996 | 8969297 |
| [chronically hiv-1 infected human monocyte culture u937 as a model for assessing the efficacy of anti-hiv agents]. | cell culture u937 chronically infected with hiv-1 is suggested as a model for adequate evaluation of antiviral activity of hiv inhibitors. azidothimidine (azt) notable decreased hiv-1 reproduction in chronically infected u937 cells to passages 15-18. glycirrhizic acid (ga) effectively inhibited the virus production during the first four passages, while in subsequent passages (up to passage 20) decreased the virus production by only 60% in comparison with the control. if a combination of azt and ... | 2001 | 11785387 |