| randomized dose range study of a recombinant hepatitis b vaccine produced in mammalian cells and containing the s and pres2 sequences. | the safety and immunogenicity of different doses (2, 5, 10, and 20 micrograms of a recombinant hepatitis b virus (rhbv) vaccine containing the s and pres2 sequences and produced in mammalian cells were compared to those of a plasma-derived hepatitis b virus vaccine (hevac b pasteur) in 482 volunteers. local and general side effects were mild and transient. no transaminase level elevation and autoantibody production were observed. the antibody to hepatitis b surface antigen (hbsag) seroconversion ... | 1989 | 2527274 |
| fine flexuous threadlike particles in cells of plants and insect hosts infected with rice hoja blanca virus. | | 1969 | 5358822 |
| capped nonviral sequences at the 5' end of the mrnas of rice hoja blanca virus rna4. | subgenomic rnas of both polarities corresponding to rice hoja blanca virus (rhbv) ambisense rna4 were detected in rhbv-infected rice tissues. total rna extracted from rhbv-infected and noninfected rice tissues and rna4 purified from rhbv ribonucleoprotein particles were used as templates for primer extension studies. the rnas extracted from rhbv-infected tissues contain a population of rna molecules with 10 to 17 nonviral nucleotides at their 5' end. the rna-cdna hybrids resulting from primer ex ... | 1995 | 7853540 |
| breaking tolerance leads to autoantibody production but not autoimmune liver disease in hepatitis b virus envelope transgenic mice. | hepatitis b virus (hbv) transgenic mice containing the hbv envelope open reading frame under the transcriptional control of the mouse albumin promoter express hepatitis b surface ag (hbsag) in all of their hepatocytes and secrete hbsag (10 to 40 ng/ml) into the circulation. because these transgenic mice show no signs of spontaneous liver cell injury or autoimmunity toward the viral (self-) ag, we asked whether the state of self-tolerance could be reversed by the induction of an acute necroinflam ... | 1995 | 7868916 |
| sequence analysis of rice hoja blanca virus rna 3. | rna 3 of rice hoja blanca tenuivirus (rhbv) has 2299 nucleotides and resembles rna 3 of other tenuiviruses such as maize stripe (mstv) and rice stripe (rstv) viruses in potentially coding with an ambisense strategy for two proteins. both the viral-sense protein of 23k and the complementary-sense protein of 35k have about 46% amino acid identity with the analogous proteins encoded by rna 3 of mstv and rstv. as the proteins of mstv and rstv have about 65% identity between themselves, rhbv cannot b ... | 1994 | 8046420 |
| approach to establishing a liver targeting gene therapeutic vector using naturally occurring defective hepatitis b viruses devoid of immunogenic t cell epitope. | the development of liver-directed virus vector may play a crucial role in hepatic gene therapy. hepatitis b virus (hbv) is the only known dna virus that has hepatocyte specificity. in order to construct an efficient hbv-based vector for targeting the liver, we studied the potential use of naturally occurring defective hbvs obtained from hepatitis patients. the enhanced green fluorescent protein (egfp) gene or small tag sequences (flag) were introduced in frame into the deleted sites of the defec ... | 2002 | 12034485 |
| hepatitis b virus vector carries a foreign gene into liver cells in vitro. | hepatitis b viruses (hbv) specifically target the liver, where they efficiently infect quiescent hepatocytes. thus, hbv virus has potential to be used as vectors for liver-directed gene transfer. we constructed a new hbv-based vector system. it is composed of transfer vector for transferring a foreign gene, green fluorescence protein (gfp) gene, and a helper vector. when the transfer vector and the helper vector were cotransfected into hepg2 cells, the recombinant hbv (rhbv) particles were gener ... | 2002 | 12086141 |
| no evidence of autoimmunity in 6-year-old children immunized at birth with recombinant hepatitis b vaccine. | taking into account that genetic predisposition, marked by human leukocyte antigen (hla) class i and ii genes, augments the probability of developing an autoimmune disorder after a triggering vaccination, as largely debated, we investigated the frequency of autoantibody production after recombinant hepatitis b vaccine (rhbv) in 6-year-old children immunized at birth to evaluate an association between autoimmune disorders and hepatitis b virus vaccination. | 2002 | 12093985 |
| negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. | posttranscriptional silencing of a green fluorescent protein (gfp) transgene in nicotiana benthamiana plants was suppressed when these plants were infected with tomato spotted wilt virus (tswv), a plant-infecting member of the bunyaviridae: infection with tswv resulted in complete reactivation of gfp expression, similar to the case for potato virus y, but distinct from that for cucumber mosaic virus, two viruses known to carry genes encoding silencing suppressor proteins. agrobacterium-based lea ... | 2003 | 12502849 |
| molecular characterization of iranian wheat stripe virus shows its taxonomic position as a distinct species in the genus tenuivirus. | the full lengths of three genome segments of iranian wheat stripe virus (iwsv) were amplified by reverse transcription (rt) followed by polymerase chain reaction (pcr) using a primer complementary to tenuivirus conserved terminal sequences. the segments were sequenced and found to comprise 3469, 2337, and 1831 nt, respectively. the gene organization of these segments is similar to that of other known tenuiviruses, each displaying an ambisense coding strategy. iwsv segments, however, are differen ... | 2006 | 16328148 |
| expression of the rice hoja blanca virus (rhbv) non-structural protein 3 (ns3) in escherichia coli and its in situ localization in rhbv-infected rice tissues. | the non-structural ns3 protein gene from the rice hoja blanca virus (rhbv) was fused to the glutathione-s-transferase carboxilic end and expressed in escherichia coli strain jm83. large quantities of fusion protein were produced in insoluble form. the fusion protein was fractionated in sds-page and purified by electroelution, polyclonal antibodies were raised in rabbit and the antiserum was absorbed with bacterial crude extract. a band of similar size as that of ns3 protein was observed in weste ... | 2004 | 17361569 |
| the ns3 protein of rice hoja blanca tenuivirus suppresses rna silencing in plant and insect hosts by efficiently binding both sirnas and mirnas. | rna silencing plays a key role in antiviral defense as well as in developmental processes in plants and insects. negative strand rna viruses such as the plant virus rice hoja blanca tenuivirus (rhbv) replicate in plants and in their insect transmission vector. like most plant-infecting viruses, rhbv encodes an rna silencing suppressor, the ns3 protein, and here it is demonstrated that this protein is capable of suppressing rna silencing in both plants and insect cells. biochemical analyses showe ... | 2007 | 17513697 |
| adenoviral delivery of recombinant hepatitis b virus expressing foreign antigenic epitopes for immunotherapy of persistent viral infection. | we previously reported a proof-of-concept study for curing chronic hepatitis b virus (hbv) infection using a foreign-antigen recombinant hbv (rhbv) as a gene therapy vector. targeted elimination of wild-type hbv (wthbv)-infected cells could be achieved by functionally activating an in situ t-cell response against the foreign antigen. however, as chronic hbv infection spreads to all hepatocytes, specific targeting of virus-infected cells is thought to be less critical. it is also feared that rhbv ... | 2014 | 24371056 |
| genetic diversity of costa rican populations of the rice planthopper tagosodes orizicolus (homoptera: delphacidae). | tagosodes orizicolus (homoptera: delphacidae) is one of the main constraints of the rice production in the neotropics. this planthopper produces severe damages as a phloem feeder, causes mechanical injury during oviposition and vectors the rice hoja blanca virus (rhbv). the main objective of this study was to determine the genetic diversity of t. orizicolus populations from three rice growing regions of costa rica, using rapds. individuals from guanacaste, parrita, san carlos and cali-colombia, ... | 2004 | 17361572 |
| nucleic acid binding property of the gene products of rice stripe virus. | gst fusion proteins of the six gene products from rnas 2,3 and 4 of the tenuivirus, rice stripe virus (rsv), were used to study the nucleic acid binding activities in vitro. three of the proteins, p3, pc3 and pc4, bound both single- and double-stranded cdna of rsv rna4 and also rna3 transcribed from its cdna clone, while p2, pc2-n (the n-terminal part of pc2) nor p4 bound the cdna or rna transcript. the binding activity of p3 is located in the carboxyl-terminus amino acid 154-194, which contains ... | 2005 | 16025246 |
| expression and inheritance of hypersensitive resistance to rice hoja blanca virus mediated by the viral nucleocapsid protein gene in transgenic rice. | rice hoja blanca virus (rhbv) is a major virus disease of economic importance affecting rice in northern south america, central america and the caribbean. this is the first report of transgenic resistance to rhbv and the transformation of an indica rice variety from latin america. rice transformed with the rhbv nucleocapsid protein ( n) gene had a significant reduction in disease development. several reactions were observed that ranged from susceptible to completely resistant plants (immunity). ... | 2003 | 12671749 |
| phylogenetic placement of a novel tenuivirus from the grass urochloa plantaginea. | evidence is presented that a tenuivirus recovered from the grass urochloa plantaginea is probably a novel tenuivirus species, to be called urochloa hoja blanca virus (uhbv). it is related to both echinochloa hoja blanca virus (ehbv) and rice hoja blanca virus (rhbv), and these three form a group distinct from maize stripe virus (mstv) and rice stripe virus (rstv). phylogenetic analysis of the sequence data for rna-3 and rna-4 of these viruses supports the hypothesis that ehbv and uhbv may have e ... | 2001 | 11450951 |
| infection of barley protoplasts with rice hoja blanca tenuivirus. brief report. | a barley protoplast system has been established that supports replication of rice hoja blanca tenuivirus (rhbv). following polyethylene glycol-mediated rhbv inoculation of barley protoplasts, newly synthesized viral rnas and proteins could be detected. time course analyses revealed de novo synthesis of genome length viral rna4, as well as subgenomic-sized rna4 molecules of both polarities. two proteins, n and ns4, encoded by viral complementary rna3 and viral rna4 respectively, were detected by ... | 1999 | 10603179 |
| comparison of colombian and costa rican strains of rice hoja blanca tenuivirus. | the sequences of rna-3 and rna-4 of rice hoja blanca tenuivirus isolates from colombia and from costa rica were determined and analyzed. these isolates were 98.9% and 98.6% identical in the coding and non-coding regions of rna-3, and 96.9 and 91.5% identical in the coding and non-coding regions of rna-4, and are therefore strains of the same virus. there is about three times as much variation between isolates (based on consensus sequences) as there is within isolates (based on sequences of indiv ... | 1997 | 9482584 |
| characterization of the in vitro activity of the rna-dependent rna polymerase associated with the ribonucleoproteins of rice hoja blanca tenuivirus. | an rna-dependent rna polymerase (rdrp) activity associated with the ribonucleoproteins of rice hoja blanca tenuivirus (rhbv) was detected and analyzed. conditions for in vitro rna synthesis and for coupled rna synthesis-translation of rhbv were established. in both cases, synthesis of the viral and viral complementary genomic and subgenomic rna3 and rna4 were observed, demonstrating that both transcription and replication occurred. though coupling of rna synthesis to translation allowed efficien ... | 1997 | 9060614 |
| sequence of echinochloa hoja blanca tenuivirus rna-3. | analysis of the sequence of the 2336 nucleotide rna-3 of echinochloa hoja blanca tenuivirus shows that it is closely related to rna-3 of rice hoja blanca tenuivirus, the principal virus disease of rice in latin america. this is especially true for the coding regions, where the viruses are almost 90% similar. however, the non-coding regions of rna-3 of these viruses, principally the intergenic region separating the two ambisense open reading frames, are only about 50% similar, suggesting that the ... | 1996 | 8938981 |
| sequence of echinochloa hoja blanca tenuivirus rna-4. | the sequence is presented of rna-4 of echinochloa hoja blanca tenuivirus (ehbv), one of two tenuiviruses associated with rice cultivation in latin america (together with rice hoja blanca virus; rhbv). analysis of the sequence shows that the coding regions of ehbv rna-4 are closely related to those of rhbv rna-4. however, the intergenic region separating the two ambisense open reading frames, are highly distinct for the two viruses. the features of the rna and the comparisons with the sequences o ... | 1996 | 8938980 |
| sequence of rice hoja blanca tenuivirus rna-2. | the sequence of rice hoja blanca tenuivirus rna-2 is analysed and compared to its counter-part in rice stripe tenuivirus. the rna encodes two proteins, in an ambisense arrangement. the 94 kd pc2, located in the complementary sense rna, has several features typical of viral membrane (glyco)proteins, and also has regions of local homology to the glycoproteins of the phleboviruses (bunyaviridae). the 23 kd pv2 lies in the viral sense rna and has two small conserved domains that are almost exclusive ... | 1996 | 8883360 |
| sequence of echinochloa hoja blanca tenuivirus rna-5. | the sequence is presented of rna-5 of echinochloa hoja blanca tenuivirus, a second tenuivirus associated with rice cultivation in latin america (after rice hoja blanca virus). the rna is 1334 nucleotides long and contains in the complementary sense rna a single long open reading frame. the deduced amino acid sequence of this open reading frame shows that it encodes a highly basic and hydrophilic 44 kd protein (pc5) with about 50% similarity to the pc5 protein of maize stripe virus (mstv). this a ... | 1996 | 8879129 |
| rapid, small scale purification of rice hoja blanca and echinochloa hoja blanca tenuivirus ribonucleoprotein. | highly purified tenuivirus ribonucleoprotein was obtained from small amounts of leaf tissue by sedimenting the ribonucleoprotein particles from debris-free plant extract into a 30% sucrose cushion, in 1.5-ml microfuge tubes. using this protocol, significant size differences were discovered in the double-stranded forms of the viral genomic rnas of rice hoja blanca tenuivirus and a tenuivirus isolated from echinochloa colonum, a common weed of rice cultivation. | 1996 | 8690758 |
| sequence of the pv2 gene of rice hoja blanca tenuivirus rna-2. | comparison of a partial sequence of rice hoja blanca tenuivirus rna-2 with 40% similarity to rice stripe tenuivirus rna-2 revealed regions of high local sequence homology at the 5' terminus, within the coding region (the pv2 gene), and in the intergenic region separating this gene from the other protein (pc2) encoded by this ambisense rna. analysis of the conserved regions of the pv2 protein identified two motifs found principally in viral membrane glycoproteins and six motifs found each in a wi ... | 1995 | 8560781 |
| comparative light and electron microscopic analyses of tenuivirus major noncapsid protein (ncp) inclusion bodies in infected plants, and of the ncp in vitro. | tenuivirus infections are associated with the formation of abundant inclusion bodies and with the accumulation of large quantities of a viral noncapsid protein (ncp) in infected plants. examination of maize stripe virus and rice hoja blanca virus-infected plant tissues using light, immunofluorescent, and electron microscopy showed that the inclusion bodies induced by the two viruses were very similar. light microscopy revealed that both induced arrays of ring-like, figure-eight-like, and amorpho ... | 1993 | 8317091 |
| complete nucleotide sequence and coding strategy of rice hoja blanca virus rna4. | the complete sequence of rice hoja blanca virus (rhbv) rna4 has been determined, based on the sequence of the corresponding cdna clones. rna4 consists of 1991 nucleotides with two open reading frames (orfs). one putative orf is located in the 5'-proximal region of the viral rna4; it encodes a protein of predicted m(r) 20076 which corresponds to the major non-structural protein that accumulates in rhbv-infected rice plants, and which bears limited sequence identity with the helper component of to ... | 1993 | 8245863 |
| rice hoja blanca virus genome characterization and expression in vitro. | no information exists on the organization and mechanisms of expression of the genome of rice hoja blanca virus (rhbv), a member of the tenuivirus group, but here we describe the first steps in its characterization. rhbv contains four ssrna and three dsrna species, the sizes of which were estimated by native and denaturing gel electrophoresis. hybridization analyses using 32p-labelled riboprobes of viral and viral complementary polarities showed that unequal amounts of the two polarities of at le ... | 1992 | 1607863 |
| in situ immunogold labeling analysis of the rice hoja blanca virus nucleoprotein and major noncapsid protein. | ribonucleoprotein particles (rnps) of rice hoja blanca virus (rhbv) were purified and used for electron microscopic analysis and antibody production. antibodies made to rnps specifically decorated purified rnps. the rnps typically showed characteristic tenuivirus morphologies. they were approximately 8 nm in diameter, mostly circular in nature, and exhibited branching and a high degree of superhelicity. when the rnp antibodies were used for in situ immunogold labeling analysis of rhbv-infected t ... | 1992 | 1448918 |
| is obesity a risk factor for vaccine non-responsiveness? | understanding the link between vaccine immunogenicity and efficacy is currently a major focus in hiv research. consequently, recent developments in the hiv-1 vaccine field have led to a closer look at immune responses to known efficacious vaccines. we undertook a study to explore clinical predictors of vaccine efficacy following recombinant hepatitis b (rhbv) vaccination in a cohort of hiv-uninfected, hepatitis b virus naïve women living in a peri-urban setting in cape town. our aim was to defin ... | 2013 | 24349359 |
| overcoming hbv immune tolerance to eliminate hbsag-positive hepatocytes via pre-administration of gm-csf as a novel adjuvant for a hepatitis b vaccine in hbv transgenic mice. | granulocyte-macrophage colony-stimulating factor (gm-csf) is known to be a potential vaccine adjuvant despite contradictory results from animal and human studies. the discrepancies may be due to the different doses and regimens of gm-csf that were used, given that either mature or immature dendritic cells (dcs) could be induced under different conditions. to test the hypothesis that gm-csf can be used as a novel adjuvant for a hepatitis b virus (hbv) therapeutic vaccine, we administered gm-csf o ... | 2016 | 26166767 |
| structural implications into dsrna binding and rna silencing suppression by ns3 protein of rice hoja blanca tenuivirus. | rice hoja blanca tenuivirus (rhbv), a negative strand rna virus, has been identified to infect rice and is widely transmitted by the insect vector. ns3 protein encoded by rhbv rna3 was reported to be a potent rnai suppressor to counterdefense rna silencing in plants, insect cells, and mammalian cells. here, we report the crystal structure of the n-terminal domain of rhbv ns3 (residues 21-114) at 2.0 å. rhbv ns3 n-terminal domain forms a dimer by two pairs of a-helices in an anti-parallel mode, w ... | 2011 | 21460234 |
| hepatitis b virus as a gene delivery vector activating foreign antigenic t cell response that abrogates viral expression in mouse models. | chronic hepatitis b virus (hbv) infection is characterized by functionally impaired t cell responses. to ensure active immunotherapy, the immune response must be switched from exhausted t cells to functional effectors that can attain the liver and cure the viral infection. we thus designed a recombinant hbv (rhbv) containing a modified viral core gene that specifically delivers a foreign antigenic polyepitope to the liver. this recombinant virus could only be self-maintained in hepatocytes alrea ... | 2009 | 19821533 |
| binding of small interfering rna molecules is crucial for rna interference suppressor activity of rice hoja blanca virus ns3 in plants. | the ns3 protein of rice hoja blanca virus represents a viral suppressor of rna interference (rnai) that sequesters small interfering (si)rnas in vitro. to determine whether this sirna binding property is the critical determinant for the suppressor activity of ns3, ns3 was altered by alanine point mutations and the resulting mutant proteins were tested for both sirna binding ability and rnai suppressor activity in plants. alanine substitutions of lysine residues at positions 173-175 resulted in m ... | 2009 | 19282433 |
| the ns3 protein of rice hoja blanca virus complements the rnai suppressor function of hiv-1 tat. | the question of whether rna interference (rnai) acts as an antiviral mechanism in mammalian cells remains controversial. the antiviral interferon (ifn) response cannot easily be distinguished from a possible antiviral rnai pathway owing to the involvement of double-stranded rna (dsrna) as a common inducer molecule. the non-structural protein 3 (ns3) protein of rice hoja blanca virus (rhbv) is an rna silencing suppressor (rss) that exclusively binds to small dsrna molecules. here, we show that th ... | 2009 | 19218918 |
| the ns3 protein of rice hoja blanca virus suppresses rna silencing in mammalian cells. | the ns3 protein of the tenuivirus rice hoja blanca virus (rhbv) has previously been shown to represent the viral rna interference (rnai) suppressor and is active in both plant and insect cells by binding short interfering rnas (sirnas) in vitro. using a firefly luciferase-based silencing assay it is described here that ns3 is also active in mammalian cells. this activity is independent of the inducer molecule used. using either synthetic sirnas or a short hairpin rna construct, ns3 was able to s ... | 2008 | 18089758 |
| high expression of hepatitis b virus based vector with reporter gene in hepatitis b virus infection system. | to construct a hepatitis b virus (hbv)-based vector with a reporter gene and to establish an hbv infection system to evaluate the availability of the vector. | 2007 | 17552034 |
| multicenter review of liver transplant for hepatitis b-related liver disease: disparities in gender and ethnicity. | orthotopic liver transplantation (olt) is the preferred treatment for selected patients with hepatitis b virus (hbv)-related liver disease. this study aimed to (i) define long-term outcomes following olt for hbv; (ii) to quantify the incidence of hbv recurrence (rhbv) as it relates to anti-hbv treatment; and (iii) to determine outcomes for specific patient subgroups. we performed a retrospective chart review of 738 patients undergoing olt between 1985 and 2010 at seven us transplant centers and ... | 2014 | 24033475 |
| novel recombinant hepatitis b virus vectors efficiently deliver protein and rna encoding genes into primary hepatocytes. | hepatitis b virus (hbv) has extremely restricted host and hepatocyte tropism. hbv-based vectors could form the basis of novel therapies for chronic hepatitis b and other liver diseases and would also be invaluable for the study of hbv infection. previous attempts at developing hbv-based vectors encountered low yields of recombinant viruses and/or lack of sufficient infectivity/cargo gene expression in primary hepatocytes, which hampered follow-up applications. in this work, we constructed a nove ... | 2013 | 23552416 |
| [micromorphology of leaf epidermis of some venezuelan rice cultivars (poaceae) associated with the mechanical damage of sogata t. orizicolus (homoptera: delphacidae)]. | rice cultivars are affected directly and indirectly by the insect sogata. the mechanical damage or direct loss, is produced after feeding and ovoposition on the young leaves tissues, while the indirect damage is produced after the transmission of the rice hoja blanca virus. we studied the morpho-anatomic structures associated with the resistance of the mechanical damage produced by the insect, in six rice cultivars, including controls for resistance and susceptibility (makalioka and bluebonnet 5 ... | 2014 | 25102661 |
| major qtls control resistance to rice hoja blanca virus and its vector tagosodes orizicolus. | rice hoja blanca (white leaf) disease can cause severe yield losses in rice in the americas. the disease is caused by the rice hoja blanca virus (rhbv), which is transmitted by the planthopper vector tagosodes orizicolus. because classical breeding schemes for this disease rely on expensive, time-consuming screenings, there is a need for alternatives such as marker-aided selection. the varieties fedearroz 2000 and fedearroz 50, which are resistant to rhbv and to the feeding damage caused by t. o ... | 2014 | 24240781 |
| mechanisms of oryza sativa (poaceae) resistance to tagosodes orizicolus (homoptera: delphacidae) under greenhouse condition in venezuela. | tagosodes orizicolus is one of the main plagues of rice in tropical america causing two types of damages, the direct one, feeding and oviposition effect, and an indirect one, by the transmission of the "rice hoja blanca virus". during 2006-2007 we carried out research under greenhouse conditions at fundaci6n danac, venezuela, in order to determine the mechanisms of antixenosis, antibiosis and tolerance to t. orizicolus, which could be acting in commercial varieties and advanced lines of the rice ... | 2012 | 22458212 |