| serological relationships between robinia mosiac, clover blotch and peanut stunt viruses. | based on the results of agar gel double immunodiffusion tests with broad spectrum rabbit antisera and narrow spectrum mouse immune ascitic fluids and formalin-fixed purified viruses, a close relationship was established between 3 members of the cucumovirus group namely robinia mosaic virus (romv), clover blotch virus (cbv) and peanut stunt virus (psv). the relationship of these 3 viruses to cucumber mosiac virus proved to be more distant. the results also suggested that romv and cbv could repres ... | 1979 | 94769 |
| inactivation of peanut stunt virus by pancreatic ribonuclease. | | 1975 | 234653 |
| use of inactivating agents to detect and partially characterize very early events in the infection of cowpea leaf epidermal cells by peanut stunt virus. | | 1976 | 948863 |
| [effect of actinomycin d on the reproduction of peanut stunt virus]. | | 1975 | 1207519 |
| [structural changes in cells of host plants induced by peanut stunt virus]. | | 1975 | 1207524 |
| nucleotide sequence analyses of peanut stunt cucumovirus rnas 1 and 2. | the nucleotide sequences of the rnas 1 and 2 of the peanut stunt virus strain j (psv-j) were determined and compared with those of the cucumber mosaic virus strain y (cmv-y, subgroup i), strain q (cmv-q, subgroup ii) and the tomato aspermy virus strain v (tav-v) at both the nucleotide and protein levels. rna 1 of psv-j consists of 3355 nucleotides (nt) and has one large open reading frame (orf) which can encode the putative 1a protein of mr 112025. psv-j rna 1 and the 1a protein are 65 to 73% id ... | 1992 | 1545224 |
| replication of cucumber mosaic virus satellite rna in vitro by an rna-dependent rna polymerase from virus-infected tobacco. | an rna-dependent rna polymerase purified from tobacco infected with cucumber mosaic virus catalyzes the synthesis of (-) and (+) strands of the viral satellite rna, carna 5, but fails to replicate the satellite rna of peanut stunt virus (psv). the enzyme replicates the genomic rnas of the three principal cucumoviruses cmv, psv and tomato aspermy virus (tav) with varying efficiencies. the specificity with which cmv rdrp replicates different sequence-unrelated rna templates suggests that the site ... | 1991 | 1720399 |
| nucleotide sequence of rna 3 of peanut stunt cucumovirus. | the nucleotide sequence of the rna 3 of the peanut stunt virus strain j (psv-j) was determined and compared with those of the cucumber mosaic virus strain y (cmv-y, subgroup i) and the tomato aspermy virus strain c (tav-c) at both the nucleotide and protein levels. the rna 3 of psv-j consists of 2186 nucleotides and has two large open reading frames (orfs). by analogy to other tripartite plant viruses, it is presumed that the first orf (867 nucleotides) codes for the 3a protein and the second or ... | 1991 | 1926787 |
| improved elisa conditions for detection of plant viruses. | clover yellow vein virus (cyvv) and homologous antisera were used to test effects of time and temperature on enzyme-linked immunosorbent assay (elisa) in polystyrene substrate plates. replicated lattice square and youden square experimental designs were used to measure and account for variation in absorption values associated with sample position within polystyrene plates. adsorption of coating antibody to polystyrene was relatively rapid, reaching optimum assay efficiency in 1 h at 5 degrees c ... | 1981 | 7021575 |
| specificity of replicase-mediated resistance to cucumber mosaic virus. | plants transformed with a nucleotide sequence coding for a truncated rna 2 replicase gene of the subgroup i strain of cucumber mosaic virus, fny-cmv, are resistant to cucumber mosaic disease. two resistant lines representing independent transformations in the original study have been propagated, and their progeny have been examined. resistance to fny-cmv was genetically integrated and was retained in the r4 generation. fourteen subgroup i cmv strains, 6 strains uncharacterized as to subgroup, an ... | 1994 | 8184532 |
| molecular characterization of an atypical old world strain of peanut stunt virus. | northern blot analyses of rnas from an iranian strain of peanut stunt virus (psv-i cucumovirus) using cloned cdna probes to the genomic rnas from two psv strains, psv-er (subgroup i) and psv-w (subgroup ii), indicated that psv-i rna-3 is derived from a subgroup ii strain. no hybridization signals, however, were detected with psv-i rnas 1 and 2 suggesting they are distinct from both subgroups i and ii. nucleotide (nt) sequence analysis of cloned cdna fragments (1-1.5 kbp in size) representing psv ... | 1999 | 10486112 |
| rt-pcr detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. | reverse transcription and polymerase chain reaction (rt-pcr) was used for detection and identification of three cucumoviruses (cucumber mosaic virus, cmv; peanut stunt virus, psv; tomato aspermy virus, tav) in various plants sources with a single pair of primers, designed as cptall-3 and cptall-5. the pair of cucumovirus genus-specific primers that flank the coat protein gene were designed and used to amplify a dna fragment of approximately ranging from 938 to 966 bp. the rt-pcr with the set of ... | 1999 | 10598084 |
| generation of infectious cdna clones of a korean strain of tomato aspermy virus. | infectious full-length cdna clones of the korean strain of tomato aspermy cucumovirus (kc-tav) were constructed using a long-template reverse transcription-polymerase chain reaction. the in vitro rna transcripts, which were produced using t7 rna polymerase from full-length cdnas, could systemically infect the nicotiana tabacum cv. xanti-nc plants and induce systemic symptoms on the upper leaves that are similar to the wildtype kc-tav. the complete nucleotide sequences of genomic rnas of kc-tav w ... | 2002 | 11911475 |
| homology modelling and protein structure based functional analysis of five cucumovirus coat proteins. | coat proteins (cp) of five cucumovirus isolates, cucumber mosaic virus (cmv) strains r, m and trk7, tomato aspermy virus (tav) strain p and peanut stunt virus (psv) strain er, were constructed by homology modelling. the x-ray structure of the fny-cmv cp subunit b was used as a template. models of cucumovirus cps were built by the modeller program. model refinements were carried out using the kollman molecular mechanical force field. models were analyzed by the procheck programs. electrostatic po ... | 2006 | 16257549 |
| identification and sequence analysis of potyviruses infecting crops in vietnam. | fifty-two virus isolates from 13 distinct potyvirus species infecting crops in vietnam were identified and the 3' region of each genome was sequenced. the viruses were: bean common mosaic virus (bcmv), potato virus y (pvy), sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv), chilli veinal mottle virus (chivmv), zucchini yellow mosaic virus (zymv), leek yellow stripe virus (lymv), shallot yellow stripe virus (sysv), onion yellow dwarf virus (oydv), turnip mosaic virus (tumv), dasheen mosa ... | 2008 | 17906829 |
| nucleotide sequence analyses of genomic rnas of peanut stunt virus mi, the type strain representative of a novel psv subgroup from china. | the complete nucleotide sequence of peanut stunt virus strain mi (psv-mi) from china was determined and compared to other viruses of the genus cucumovirus. the tripartite genome of psv-mi encoded five open reading frames (orfs) typical of cucumoviruses. distance analyses of four orfs indicated that psv-mi differed sufficiently in nucleotide sequence from other psv strains of subgroups i and ii to warrant establishment of a third subgroup of psv. | 2005 | 15747049 |
| interaction of replicase components between cucumber mosaic virus and peanut stunt virus. | cucumber mosaic virus (cmv) and peanut stunt virus (psv) each have genomes consisting of three single-stranded rna molecules: rna 1, 2 and 3. rnas 1 and 2 encode the 1a and 2a proteins, respectively, which are necessary for replication of the viral genome. although rna 3 is exchangeable between cmv and psv, exchange of rna 1 and 2 between the two viruses has been unsuccessful. in this study, reassortants containing psv rna 1 and cmv rna 2 together with rna 3 of cmv or psv were shown to be able t ... | 2003 | 12810890 |
| production of infectious rna transcripts from full-length cdna clones representing two subgroups of peanut stunt virus strains: mapping satellite rna support to rna1. | full-length cdna clones from which infectious transcripts could be generated were constructed from the genomic rnas of two distinct strains of peanut stunt cucumovirus (psv), psv-er and psv-w. psv-er, a subgroup i strain, is known to support efficient replication of satellite rna (satrna) in infected plants, whereas psv-w, a subgroup ii strain, does not support satrna replication. although artificial reassortants (pseudorecombinants) of all possible combinations of infectious transcripts represe ... | 1998 | 9714252 |
| differential interactions among isolates of peanut stunt cucumovirus and its satellite rna. | the interactions of seven isolates of peanut stunt cucumovirus (psv) originating from north america, europe and africa, and two variants of psv satellite rna (sat rna) were analysed. electrophoretic and immunoblot analyses of the coat protein (cp) and northern blot hybridization analyses of the viral rnas showed that isolates psv f352, 1339 and 1507 belonged to subgroup i, and isolates psv w, su and b to subgroup ii. the seventh isolate, robinia mosaic virus (romv) clustered with subgroup i isol ... | 1998 | 9460940 |
| evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of rna3. | strains of peanut stunt cucumovirus (psv) were classified into two distinct subgroups, i and ii, based on western and northern blot analyses using antisera and cloned cdna probes to strains psv-er and psv-w. these results were corroborated by nucleotide sequence analyses of full-length cdna clones of rna3 from representative strains of the two subgroups. whereas the percentage nucleotide sequence identity between psv-er (or psv-j) and psv-w rna3s was determined to be 80%, the corresponding value ... | 1997 | 9129668 |
| characterization of defective rnas derived from rna 3 of the fny strain of cucumber mosaic cucumovirus. | two defective rnas (designated d rna 3 alpha and d rna 3 beta) were found to be associated with the fny strain of cucumber mosaic cucumovirus but not with the sny strain after serial passages in a tobacco host. the d rnas were derived from rna 3 by single, in-frame deletions within the 3a open reading frame. a full-length cdna clone from which biologically active transcripts can be produced in vitro has been constructed for d rna 3 beta. this transcript can be replicated in tobacco plants infect ... | 1995 | 7609040 |
| cucumber mosaic virus, a model for rna virus evolution. | summary taxonomic relationships: cucumber mosaic virus (cmv) is the type member of the cucumovirus genus, in the family bromoviridae. additional members of the genus are peanut stunt virus (psv) and tomato aspermy virus (tav). the rnas 3 of all members of the genus can be exchanged and still yield a viable virus, while the rnas 1 and 2 can only be exchanged within a species. physical properties: the virus particles are about 29 nm in diameter, and are composed of 180 subunits (t = 3 icosahedral ... | 2001 | 20572992 |
| tissue blot immunoassay and direct rt-pcr of cucumoviruses and potyviruses from the same nitropure nitrocellulose membrane. | a method is described for using nitropure nitrocellulose (npn) membranes as an effective solid matrix for retrieval of template rna of three potyviruses, tobacco etch virus, soybean mosaic virus and turnip mosaic virus, and two cucumoviruses, cucumber mosaic virus and peanut stunt virus. these npn membranes were also used for tissue blot immunosorbent assays (tbias) to identify and detect plant viruses. for rna detection, discs from dried membranes blotted with infected tissue were minimally cle ... | 2010 | 21126542 |
| Cucumber mosaic virus: viral genes as virulence determinants. | Taxonomic relationships: Cucumber mosaic virus (CMV) is the type species of the genus Cucumovirus in the family Bromoviridae, which also encompasses the Peanut stunt virus (PSV) and the Tomato aspermy virus (TAV). Nucleotide sequence similarity among these three cucumoviruses is 60%-65%. CMV strains are divided into three subgroups, IA, IB and II, based on the sequence of the 5' untranslated region of the genomic RNA 3. Overall nucleotide sequence similarity among CMV strains is approximately 70 ... | 2011 | 21980997 |
| replication of satellite rna in vitro by homologous and heterologous cucumoviral rna-dependent rna polymerases. | the rna-dependent rna polymerases (rdrp) of cucumber mosaic virus (cmv) and peanut stunt virus (psv), members of the cucumovirus group, have been purified from virus infected plants and were used to study rna synthesis in vitro using different viral rnas, two cucumoviral satellites, and chimeric satellite cdna clone transcripts as templates. the results show that solubilized rdrp preparations of cmv and psv have a high degree of template dependency and catalyze (-) strand synthesis of the homolo ... | 1993 | 7506938 |
| control of virus diseases in soybeans. | soybean, one of the world's most important sources of animal feed and vegetable oil, can be infected by numerous viruses. however, only a small number of the viruses that can potentially infect soybean are considered as major economic problems to soybean production. therefore, we consider management options available to control diseases caused by eight viruses that cause, or have the potential to cause, significant economic loss to producers. we summarize management tactics in use and suggest di ... | 2014 | 25410106 |
| a novel set of polyvalent primers that detect members of the genera bromovirus and cucumovirus. | rapid detection and diagnosis of plant virus infection is one of the most important steps in preventing damages caused by viral diseases. bromoviruses and cucumoviruses belong to the family bromoviridae, which is one of the most important families of plant viruses, and infect a broad range of host plants including various economically important crops. in this study, an rt-pcr assay was developed for the universal detection of bromoviruses and cucumoviruses using a set of primers designed to targ ... | 2014 | 24717165 |
| nucleotide sequence relationships among thirty peanut stunt virus isolates determined by competition hybridization. | the nucleotide sequence homology among 30 peanut stunt virus (psv) isolates was determined. the isolates divided into two groups with little homology between them but extensive homology among their members. only one isolate had nonoverlapping partial homology with both groups. | 1983 | 6838382 |
| separation of the complementary strands of double-stranded cucumber mosaic virus-associated rna 5 and peanut stunt virus-associated rna 5. | the double-stranded forms of carna 5 and parna 5, viral satellites of the cucumovirus group, after denaturation and polyacrylamide gel electrophoresis under appropriate conditions allow their complementary strands to be separated and fractionated. these fractionated (+) and (-) strands are free of contaminating viral rna fragments. they serve as templates for the preparation of cdnas of opposite polarity, which are used to probe the viral rna-dependent replication of carna 5 and parna 5. | 1983 | 6651844 |
| replication of peanut stunt virus and its associated rna 5 in cowpea protoplasts. | peanut stunt virus (psv) rna containing psv-associated rna 5 (parna 5) was used as the inoculum in the successful infection of cowpea protoplasts. total nucleic acid extracts of protoplast samples at different times after inoculation were analyzed for the presence of psv genomic rnas and parna 5 using glyoxal denaturation, agarose gel electrophoresis, blotting to nitrocellulose, and hybridization to specific probes. it appears that (+)-stranded psv genomic rnas are synthesized up to 36 hr after ... | 1984 | 6516215 |
| host range, purification, and properties of the western strain of peanut stunt virus. | | 1969 | 5377736 |
| nucleotide sequence of the satellite of peanut stunt virus reveals structural homologies with viroids and certain nuclear and mitochondrial introns. | peanut stunt virus-associated rna 5 (parna 5), the satellite of a plant cucumovirus, is a linear rna of 393 nucleotides with a 5' cap and a 3' hydroxyl group. determination of its nucleotide sequence has revealed two consecutive open reading frames that together extend most of its length. sequences at the 5' and 3' ends are homologous with those of the satellite of the related cucumber mosaic virus, and the double-stranded forms of both satellites contain an unpaired guanosine at the 3' end of t ... | 1985 | 3858808 |
| double-stranded cucumovirus associated rna 5: experimental analysis of necrogenic and non-necrogenic variants by temperature-gradient gel electrophoresis. | cucumber mosaic virus (cmv) and peanut stunt virus (psv) each contain a fifth major rna in the size range of 334 to 393 nucleotides. this fifth rna is a satellite capable of modulating the expression of viral disease symptoms. it is present in infected tissue in single-stranded and double-stranded form. nucleotide sequence variants of the double-stranded cmv-associated rna 5 (dscarna 5) and psv-associated rna 5 (dsparna 5) were analysed by temperature-gradient gel electrophoresis. gels were 5% p ... | 1987 | 3601667 |
| detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia. | a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ... | 2017 | 28855829 |
| nucleotide sequence analysis of a cdna clone encoding the coat protein gene of peanut stunt virus. | | 1991 | 1868220 |
| symptom-modulating properties of peanut stunt virus satellite rna sequence variants. | the symptom-modulating properties of three peanut stunt virus (psv) satellite rna (satrna) sequence variants were studied. the (v)-satrna did not affect symptom development in tobacco plants infected with psv. the (g)- or (wc)-satrna, on the other hand, attenuated the symptoms. in these plants, the symptoms of psv were restricted primarily to the inoculated leaves, and in some cases, a few leaves above the inoculated leaf showed small chlorotic areas. northern blot analysis of total nucleic acid ... | 1991 | 1718510 |
| peanut stunt virus satellite rna: analysis of sequences that affect symptom attenuation in tobacco. | the v-satellite rna (v-satrna) of peanut stunt virus (psv) has no effect on symptoms produced in tobacco by psv. in contast, the g-satrna induced complete or nearly complete suppression of systemic symptom development. because g-satrna differs from v-satrna in only five nucleotide positions, these two satrnas provide excellent material for investigating the molecular basis of satrna-mediated symptom attenuation. for this purpose, we constructed transcription vectors containing full-length cdna c ... | 1992 | 1641984 |
| physical and chemical differentiation of three strains of cucumber mosaic virus and peanut stunt virus. | | 1976 | 982815 |
| peanut stunt virus-associated rna 5: second tripartite genome virus with an associated satellite-like replicating rna. | | 1978 | 676076 |
| isolation of viral double-stranded rnas using a licl fractionation procedure. | a general procedure for the isolation of virus-specific double-stranded rna (ds-rna) is discribed. the procedure is based on the differential solubility of different types of nucleic acids in licl. principal advantages over conventional methods are simplicity, avoidance of enzymatic treatment, and relatively good yields of undegraded ds-rna while permitting separation of several main groups of cellular and viral nucleic acids from the same batch of tissue. the method has been successfully applie ... | 1978 | 643823 |
| cucumber mosaic virus-associated rna 5. vi. characterization and denaturation-renaturation behavior of the double-stranded form. | the double-stranded form of cucumber mosaic virus-associated rna 5 has been purified and further characterized. its molecular weight determined by sedimentation equilibrium is 2.15 . 10(5). the buoyant density calculated from its symmetrical distribution in cs2so4, following isopycnic ultracentrifugation, is 1.615 g/cm3. the sedimentation rate of double-stranded cucumber mosaic virus-associated rna 5 is slightly greater than that of cucumber mosaic virus-associated rna 5; its electrophoretic mob ... | 1979 | 486481 |
| expression of the peanut stunt virus coat protein gene is essential and sufficient for production of host-dependent ribbon-like inclusions in infected plants. | abstract we previously have reported that infection of tobacco protoplasts or leaf tissue with the cucumovirus peanut stunt virus (psv) induced the production of unusual cytoplasmic ribbon-like inclusions. the formation of these novel inclusions is strain-specific, because infection of tobacco with subgroup ii psv strains, but not subgroup i strains, induced the production of inclusions. furthermore, we have demonstrated that induction of the ribbon-like inclusions maps to psv subgroup ii rna3, ... | 2004 | 18943904 |
| peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in nicotiana benthamiana. | to analyze the role of the 2b protein (2bp) of peanut stunt virus (psv) in the viral infection cycle, we constructed psv mutants that express either no 2bp or n-terminal-truncated 2bp. the accumulation of wild-type and mutant viruses in tobacco protoplasts indicated that the 2b cistron is not essential for viral replication. viral accumulation in nicotiana benthamiana plants suggested that the 2b cistron is responsible for viral accumulation in inoculated and upper leaves and has a role in virul ... | 2008 | 18663407 |
| cucumber mosaic virus and peanut stunt virus differ in an initial event that occurs during the infection of cowpea leaf epidermal cells. | using procedures described earlier for peanut stunt virus (psv) (g. i. mink, virology 72, 291-298, 1976), hydroxylamine, ribonuclease (rnase), and tetrachloro-o-benzoquinone were used to determine 10-min inactivation profiles for cucumber mosaic virus (cmv) and cmv-rna. these profiles indicate that the first event that is detectable by the use of inactivating agents in the infection of cowpea primary leaves by either cmv or cmv-rna differed significantly from that reported earlier for psv and ps ... | 1980 | 18631664 |
| nucleotide sequence analysis of peanut stunt virus rp strain suggests the role of homologous recombination in cucumovirus evolution. | the complete nucleotide (nt) sequence of peanut stunt virus robinia strain (psv-rp) was determined and compared to other psv strains and to representatives of the genus cucumovirus. nt sequence comparison showed 74.1-84.6% identity with the known psv strains. phylogenetic analysis revealed the different origin of the two genes encoded by rna3. while the 3a gene clustered with psv-w, the coat protein gene clustered with psv-mi. recombination breakpoint analysis revealed two recombination points o ... | 2008 | 18523838 |
| the sequence and model structure analysis of three polish peanut stunt virus strains. | peanut stunt virus (psv) belongs to the cucumovirus genus of the family bromoviridae and is widely distributed worldwide, also in poland. psv is a common pathogen of a wide range of economically important plants. its coat protein (cp), similarly as in other viruses, plays an important role in many processes during viral life cycle and has great impact on the infectivity. in this study, we present the results of sequence-structure analysis of cp derived from three polish strains of psv: psv ag, g ... | 2008 | 18049887 |
| unusual cytoplasmic inclusions induced in tobacco by peanut stunt virus subgroup ii strains map to rna3. | abstract infection of tobacco protoplasts or leaf tissues with peanut stunt virus (psv) subgroup ii strains induced the production of unusual cytoplasmic ribbon-like inclusions. the inclusion structures appeared as long, thin, densely staining sheets that were prevalent within the cytoplasm, accumulating most commonly near vacuoles. numerous virions and ribosomes could be seen adjacent to the inclusion surfaces. the formation of these novel inclusions appeared to be subgroup specific, since infe ... | 1998 | 18944853 |
| molecular evidence that strain bv-15 of peanut stunt cucumovirus is a reassortant between subgroup i and ii strains. | abstract in northern hybridization assays, rna1 of peanut stunt virus (psv) strain bv-15 hybridized strongly with a cloned cdna probe to rna1 from strain psv-w (subgroup ii). cloned probes to psv-w rna2 and rna3, however, did not hybridize with the corresponding rnas from strain bv-15. the complete nucleotide sequence of psv-bv-15 rna2 has been determined, and sequence comparison analysis showed that it is closely related to psv subgroup i strains; the percent nucleotide sequence identity betwee ... | 1998 | 18944976 |
| complete nucleotide sequence of a polish strain of peanut stunt virus (psv-p) that is related to but not a typical member of subgroup i. | peanut stunt virus (psv) is a common legume pathogen present worldwide. it is also infectious for many other plants including peanut and some vegetables. viruses of this species are classified at present into three subgroups based on their serology and nucleotide homology. some of them may also carry an additional subviral element - satellite rna. analysis of the full genome sequence of a polish strain - psv-p - associated with satrna was performed and showed that it may be classified as a deriv ... | 2008 | 19081851 |
| response of trifolium repens clones to infection by meloidogyne incognita and peanut stunt virus. | the responses of selected clones of white clover (trifolium repens) to simultaneous infection by the southern root-knot nematode (meloidogyne incognita) and peanut stunt virus (psv) were determined. two white clover clones, which were resistant (nc-r) or sensitive (nc-s) to ozone injury, were evaluated. plant growth and m. incognita reproduction were measured. root, stolon, and top growth were reduced by psv infection, which affected nc-r more than nc-s. both clones were tolerant of m. incognita ... | 1993 | 19279855 |
| symptom induction and rna silencing suppression by the cucumber mosaic virus 2b protein. | the 2b protein encoded by cucumber mosaic virus (cmv) and other cucumoviruses is multifunctional, having roles in local and systemic virus movement, symptom determination, evasion of defense mediated by salicylic acid, and in suppression of antiviral rna silencing. it also perturbs silencing-mediated regulation of host transcripts, suggesting that another function of 2b protein is to manipulate host gene expression and physiology in a way that may aid the virus. the 2b proteins encoded by the va ... | 2010 | 20404501 |
| recombination-based generation of the agroinfectious clones of peanut stunt virus. | full-length cdna clones of peanut stunt virus strain p (psv-p) were constructed and introduced into nicotiana benthamiana plants via agrobacterium tumefaciens. the cdna fragments corresponding to three psv genomic rnas and satellite rna were cloned into pgreen binary vector between cauliflower mosaic virus (camv) 35s promoter and nopaline synthase (nos) terminator employing seamless recombinational cloning system. the plasmids were delivered into a. tumefaciens, followed by infiltration of hosts ... | 2016 | 27659243 |
| comparative analysis of rna silencing suppression activities between viral suppressors and an endogenous plant rna-dependent rna polymerase. | rna silencing is an evolutionarily conserved system that functions as an antiviral mechanism in eukaryotes, including higher plants. to counteract this, several plant viruses express silencing suppressors that inhibit rna silencing in host plants. here, we show that both 2b protein from peanut stunt virus (psv) and a hairpin construct (designated hp-rdr6) that silences endogenous rna-dependent rna polymerase 6 (rdr6) strongly suppress rna silencing. the agrobacterium infiltration system was used ... | 2012 | 22354861 |
| effect of temperature on the pathogenesis, accumulation of viral and satellite rnas and on plant proteome in peanut stunt virus and satellite rna-infected plants. | temperature is an important environmental factor influencing plant development in natural and diseased conditions. the growth rate of plants grown at c27°c is more rapid than for plants grown at 21°c. thus, temperature affects the rate of pathogenesis progression in individual plants. we have analyzed the effect of temperature conditions (either 21°c or 27°c during the day) on the accumulation rate of the virus and satellite rna (satrna) in nicotiana benthamiana plants infected by peanut stunt v ... | 2015 | 26579153 |
| how can plant virus satellite rnas alter the effects of plant virus infection? a study of the changes in the nicotiana benthamiana proteome after infection by peanut stunt virus in the presence or absence of its satellite rna. | peanut stunt virus (psv), which belongs to the cucumovirus genus, is a pathogen of legumes. certain psv strains associated with a satellite rna (satrna) modify the symptoms of infected plants and interfere with plant metabolism. we used psv-p genomic transcripts (gts) with and without psv-p satrna and a comparative proteomic 2d-dige/ms study to assess their effects on nicotiana benthamiana infection. when the proteomes of the psv-p genomic transcripts-infected (no satrna present) and mock-inocul ... | 2013 | 23580405 |
| screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative rpsv1 resistance gene. | the peanut stunt virus (psv) causes yield losses in soybean and reduced seed quality due to seed mottling. the objectives of this study were to determine the phenotypic reactions of soybean germplasms to inoculation with two psv isolates (psv-k, psv-t), the inheritance of psv resistance in soybean cultivars, and the locus of the psv resistance gene. we investigated the psv resistance of 132 soybean cultivars to both psv isolates; of these, 73 cultivars exhibited resistance to both psv isolates. ... | 2012 | 23136501 |
| analysis of two strains of peanut stunt virus: satrna-associated and satrna free. | peanut stunt virus (psv) is a pathogen of legumes, vegetables, trees, and weeds occurring worldwide. the species is characterized by significant genetic variability. psv strains are classified into four subgroups on the basis of their nucleotide sequence homology. here, we are presenting two further, fully sequenced psv strains-psv-ag and psv-g, that could be considered as i subgroup representatives. however, their sequence homology with other typical i subgroups members, similarly as another st ... | 2012 | 22392626 |
| corrigendum: effect of temperature on the pathogenesis, accumulation of viral and satellite rnas and on plant proteome in peanut stunt virus and satellite rna-infected plants. | [this corrects the article on p. 903 in vol. 6, pmid: 26579153.]. | 2016 | 27375674 |