| two complete genome sequences of squash mosaic virus from 20-year-old cucurbit leaf samples from australia. | we present the first complete australian squash mosaic virus (sqmv) genome sequences. we compared the 2 australian genomes from 20-year-old cucurbit samples with 8 other sqmv genomes. the australian genomes shared >99.0% nucleotide identities, and their rna1 and rna2 sequences most closely resembled isolates y and kimbe from japan, respectively. | 2017 | 28798181 |
| comparative analysis of rna silencing suppression activities between viral suppressors and an endogenous plant rna-dependent rna polymerase. | rna silencing is an evolutionarily conserved system that functions as an antiviral mechanism in eukaryotes, including higher plants. to counteract this, several plant viruses express silencing suppressors that inhibit rna silencing in host plants. here, we show that both 2b protein from peanut stunt virus (psv) and a hairpin construct (designated hp-rdr6) that silences endogenous rna-dependent rna polymerase 6 (rdr6) strongly suppress rna silencing. the agrobacterium infiltration system was used ... | 2012 | 22354861 |
| occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran. | during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ... | 2008 | 18564898 |
| cowpea mosaic virus-encoded protease does not recognize primary translation products of m rnas from other comoviruses. | the protease encoded by the large (b) rna segment of cowpea mosaic virus was tested for its ability to recognize the in vitro translation products of the small (m) rna segment from the comoviruses squash mosaic virus, red clover mottle virus, and cowpea severe mosaic virus (cpsmv, strains dg and ark), and from the nepovirus tomato black ring virus. like m rna from cowpea mosaic virus, the m rnas from squash mosaic virus, red clover mottle virus, cpsmv-dg, and cpsmv-ark were all translated into t ... | 1982 | 16789228 |
| the coat protein genes of squash mosaic virus: cloning, sequence analysis, and expression in tobacco protoplasts. | complementary dna of the middle-component rna of the melon strain of squash mosaic comovirus (sqmv) was cloned. clones containing the coat protein genes were identified by hybridization with a degenerate oligonucleotide synthesized according to the amino acid sequence of a purified peptide fragment of the sqmv large coat protein. a clone containing of 2.5 kbp cdna insert of sqmv m-rna was sequenced. the total insert sequence of 2510 bp included a 2373 bp open reading frame (orf) (encoding 791 am ... | 1993 | 8503782 |
| poliovirus rna-dependent rna polymerase synthesizes full-length copies of poliovirion rna, cellular mrna, and several plant virus rnas in vitro. | the poliovirus rna-dependent rna polymerase was active on synthetic homopolymeric rna templates as well as on every natural rna tested. the polymerase copied polyadenylate. oligouridylate [oligo(u)], polycytidylate . oligoinosinate, and polyinosinate. oligocytidylate templates to about the same extent. the observed activity on polyuridylate. oligoadenylate was about fourfold less. full-length copies of both poliovirion rna and a wide variety of other polyadenylated rnas were synthesized by the p ... | 1982 | 6183446 |
| electron microscopy of structural detail in frozen biological specimens. | a procedure is described whereby preshadowed replicas can be obtained from frozen biological specimens which have been cut and then etched by sublimation of the ice from their surfaces. electron micrographs showing details of the internal structure of plant virus crystals are presented to demonstrate the values of the procedure. crystals of purified tobacco ringspot virus and squash mosaic virus and some portions of turnip yellow mosaic virus crystals have been shown to exhibit hexagonal packing ... | 1957 | 13416310 |
| mapping of the two coat protein genes on the middle rna component of squash mosaic virus (comovirus group). | squash mosaic virus, a member of the comovirus group, has a divided genome identified as middle-component rna (m rna; mw = 1.4 x 10(6)) and bottom-component rna (b rna; mw = 2 x 108). the isometric capsid of squash mosaic virus is composed of two distinct protein monomers with molecular weights of 22,000 (22k) and 42k. the isolated rna components were translated in a rabbit reticulocyte lysate system. translation products of the b rna had estimated molecular weights of 190k, 51k, and 32k, while ... | 1981 | 18635086 |
| localization of transmissible and nontransmissible viruses in the vector nematode xiphinema americanum. | abstract the inner lining of the food canal of nematodes that transmit plantinfecting viruses is regarded as the retention region of viruses. to characterize the location of transmissible and nontransmissible viruses in the vector nematode xiphinema americanum, three nepoviruses, tobacco ringspot virus (trsv), tomato ringspot virus(tomrsv), and cherry leaf roll virus(clrv), and one non-nematode-transmissible virus, squash mosaic virus (sqmv), were evaluated for transmission efficiency and locali ... | 2002 | 18944262 |
| the histological localization of squash mosaic virus in cantaloupe seedlings. | | 1970 | 4918265 |
| purification and electron microscopy of squash mosaic virus. | | 1969 | 5374244 |
| genome-associated proteins of comoviruses. | previously, a protein has been reported to be associated with the rnas of cowpea mosaic virus. here we present evidence for genome-associated proteins of other comoviruses: squash mosaic virus, echtes ackerbohnemosaik-virus, and another strain of cowpea mosaic virus. although the proteins and protein-oligonucleotide complexes derived from the rnas of these viruses showed similar patterns of electrophoretic mobility, two-dimensional chromatograms of their tryptic peptides were distinct. chromatog ... | 1979 | 18631616 |
| diversity among isolates of squash mosaic virus. | cdna clones of rna-2 of two isolates of squash mosaic virus (sqmv) were constructed and sequenced, revealing 87% sequence similarity. in northern blot hybridization analyses, dna probes made from these clones defined two sqmv hybridization subgroups. this grouping was verified by reciprocal hybridizations of purified rna from five sqmv isolates, as probed with cdna made from a member of each subgroup. comparison of the rna-2 sequence among the two sqmv isolates, and the reported sequence of othe ... | 1998 | 9780037 |
| evidence that resistance in squash mosaic comovirus coat protein-transgenic plants is affected by plant developmental stage and enhanced by combination of transgenes from different lines. | three transgenic lines of squash hemizygous for the coat protein genes of squash mosaic virus (sqmv) were shown previously to have resistant (sqmv-127), susceptible (sqmv-22) or recovery (sqmv-3) phenotypes. post-transcriptional gene silencing (ptgs) was the underlying mechanism for resistance of sqmv-127. here, experiments conducted to determine the mechanism of the recovery phenotype and whether enhanced resistance could be obtained by combining transgenes from susceptible and recovery plants ... | 2000 | 10950989 |
| nucleotide sequence of a japanese isolate of squash mosaic virus. brief report. | the complete nucleotide sequence of the rna-1 of squash mosaic virus (sqmv) was determined using a japanese isolate (y-sqmv). the sequence consisted of 5865 nucleotides excluding the poly (a) at the 3' terminus and contained a single long open reading frame with a coding capacity for a protein of mr209971. analysis of the deduced amino acid sequence suggested a genomic organization typical of comoviruses. the nucleotide sequence of the rna-2 of y-sqmv was also determined and compared with the sq ... | 2002 | 11890535 |
| the genomic sequence of a chinese isolate of squash mosaic virus with novel 5' conserved ends. | | 2009 | 19330435 |
| complete genome sequence of a novel genotype of squash mosaic virus infecting squash in spain. | the complete genome sequence of a new isolate of squash mosaic virus (sqmv) infecting squash plants in spain was obtained using deep sequencing of small rnas. the low nucleotide sequence identities, with only 87 to 88% for rna1 and 84 to 86% for rna2 to known sqmv isolates, suggested that this isolate belongs to a novel genotype. | 2015 | 25676773 |
| an electron microscope study of squash mosaic virus. | | 1947 | 20252517 |
| crystallization of squash mosaic virus. | | 1948 | 18913367 |
| biochemical and biophysical properties of squash mosaic virus and related macromolecules. | | 1962 | 14471880 |
| antiviral activity of thuja orientalis extracts against watermelon mosaic virus (wmv) on citrullus lanatus. | watermelon mosaic potyvirus (wmv) is considered as an important virus infecting watermelon and causing adverse effects on crop productivity. to overcome this problem one of the main objectives of plant breeders is to make these strains less effective in the ability to infect plants by treatment with plant extracts. due to the advantages of plant tissue culture, in vitro, in the process of the selection of different cultivars under biotic stress, this study was conducted to achieve this aim by ev ... | 2015 | 25737655 |