| cloned dna copies of cowpea severe mosaic virus genomic rnas: infectious transcripts and complete nucleotide sequence of rna 1. | cowpea severe mosaic virus (cpsmv) is a member of the comovirus group of messenger-sense rna viruses with bipartite genomes, of which cowpea mosaic virus (cpmv) is the type member. full-length copies of cpsmv rna 1 were cloned in plasmids bearing a bacteriophage t7 promoter. previously, similar clones of cpsmv rna 2 had been obtained. a 5'-ruauuaaaauuuu sequence is common to rna 1 and rna 2. from two rna 1 clones and four rna 2 clones we excised non-cpsmv sequences so as to provide templates for ... | 1992 | 1448917 |
| effects of ph and ionic strength on precipitation of phytopathogenic viruses by polyethylene glycol. | the effects of ionic strength of the solution (changed by varying nacl concentrations or buffer molarity) on the precipitation with polyethylene glycol (peg) 6000 were studied on phytopathogenic viruses of different morphology: the isometric red clover mottle virus (rcmv), rod-shaped tobacco mosaic virus, flexuous potato virus x (pvx) and bacilliform alfalfa mosaic virus. with increasing nacl concentration or buffer molarity up to a certain level (0.1 mol/l), the efficiency of peg precipitation ... | 1989 | 2565676 |
| effect of mg2+ ions on the in vitro translation of red clover mottle virus m rna. | red clover mottle virus middle-component rna was translated in rabbit reticulocyte lysate into two primary polypeptides with molecular weights of 95,000 and 105,000. the relative ratio of the two polypeptides synthesized was affected by mg2+ concentration. | 1987 | 2894144 |
| comparison of the effects of chlorite-oxidized oxyamylose and polyacrylic acid on the multiplication of phytopathogenic viruses. | polyacrylic acid (paa) and chlorite-oxidized oxyamylose (coam) inhibit the multiplication of tobacco mosaic virus (tmv) in leaf disks by up to 50%. the reduction in tmv content is time-dependent and decreases with longer time intervals between the virus infection and the application of substances. the multiplication of potato virus x (pvx) in leaf disks is not affected by either paa or coam. in intact plants paa produces a strong antiviral effect on both pvx and red clover mottle virus (rcmv). t ... | 1985 | 4062284 |
| effect of lysolecithin analogues on plant viruses. | newly synthesized lysolecithin analogues 2-0-hexadecyl-glycero-3-phosphocholine (1) and its methyl (2), ethyl (3) and benzyl (4) derivatives were tested with regard to the anti- phytoviral effect on potato virus x ( pvx ), red clover mottle virus ( rcmv ) and tobacco mosaic virus (tmv). the first two compounds (1, 2) reduced markedly the content of pvx and rcmv in systemically infected host plants as evidenced by precipitin test and bio-assay. whereas compounds (1), (2) and (3) did not significa ... | 1984 | 6145345 |
| determination of broad bean stain virus serotypes by enzyme-linked immunosorbent assay. | enzyme-linked immunosorbent assay (elisa) was used to determine the degree of serological specificity of two serotypes of broad bean stain virus (bbsv) and their relationship to red clover mottle virus (rcmv). optimal conditions for the differentiation by elisa of the two viruses and the two bbsv serotypes were established. bbsv isolates from vicia sativa belonged to serotype i, those from pea plants (f1, kow 60) to serotype ii, and isolates from lens culinaris differed from these two serotypes. ... | 1993 | 7905241 |
| the coat protein genes of squash mosaic virus: cloning, sequence analysis, and expression in tobacco protoplasts. | complementary dna of the middle-component rna of the melon strain of squash mosaic comovirus (sqmv) was cloned. clones containing the coat protein genes were identified by hybridization with a degenerate oligonucleotide synthesized according to the amino acid sequence of a purified peptide fragment of the sqmv large coat protein. a clone containing of 2.5 kbp cdna insert of sqmv m-rna was sequenced. the total insert sequence of 2510 bp included a 2373 bp open reading frame (orf) (encoding 791 am ... | 1993 | 8503782 |
| the 24 kda proteinases of comoviruses are virus-specific in cis as well as in trans. | to investigate the specificity of comoviral 24 kda ('24k') proteinases, a full-length cdna copy of red clover mottle virus (rcmv) rna 1 has been cloned downstream of a t7 promoter. translation in rabbit reticulocyte lysates of in vitro transcripts from this clone resulted in the synthesis of a 200k protein which was processed in a manner similar to that of the equivalent protein from cowpea mosaic virus (cpmv). full-length cdna clones of the rna 1 molecules of rcmv and cpmv were used to create h ... | 1996 | 8811039 |
| complete nucleotide sequence of bean pod mottle virus rna1: sequence comparisons and evolutionary relationships to other comoviruses. | the complete nucleotide sequence of bean pod mottle comovirus (bpmv) rna 1 was determined. it is 5983 nucleotides long, excluding the poly(a) tail, and encodes a polyprotein of 1850 amino acid (aa) residues. multiple alignments of the deduced aa sequence of bpmv polyprotein with those of cowpea mosaic virus (cpmv), red clover mottle virus (rcmv) and cowpea severe mosaic virus (cpsmv) indicated that bpmv rna1 encodes the predicted set of five mature proteins: the equivalent of cpmv 32k protease c ... | 1999 | 10403699 |
| structural fingerprinting: subgrouping of comoviruses by structural studies of red clover mottle virus to 2.4-a resolution and comparisons with other comoviruses. | red clover mottle virus (rcmv) is a member of the comoviruses, a group of picornavirus-like plant viruses. the x-ray structure of rcmv strain s has been determined and refined to 2.4 a. the overall structure of rcmv is similar to that of two other comoviruses, cowpea mosaic virus (cpmv) and bean pod mottle virus (bpmv). the sequence of the coat proteins of rcmv strain o were modeled into the capsid structure of strain s without causing any distortion, confirming the close resemblance between the ... | 2000 | 10590139 |
| cowpea mosaic virus-encoded protease does not recognize primary translation products of m rnas from other comoviruses. | the protease encoded by the large (b) rna segment of cowpea mosaic virus was tested for its ability to recognize the in vitro translation products of the small (m) rna segment from the comoviruses squash mosaic virus, red clover mottle virus, and cowpea severe mosaic virus (cpsmv, strains dg and ark), and from the nepovirus tomato black ring virus. like m rna from cowpea mosaic virus, the m rnas from squash mosaic virus, red clover mottle virus, cpsmv-dg, and cpsmv-ark were all translated into t ... | 1982 | 16789228 |
| enhanced infectivity of combined bottom and middle components of red clover mottle virus. | | 2013 | 4385168 |
| complete nucleotide sequence of radish mosaic virus rna polymerase gene and phylogenetic relationships in the genus comovirus. | the 3'-terminal part of rna1 genome segment of radish mosaic virus (ramv) including complete rna polymerase gene was sequenced. the 207 amino acids long polymerase is matured from a polyprotein precursor by cleavage at putative q/h site by viral protease. the alignment of available amino acid sequences of rna polymerase genes of comoviruses revealed a closest (55%) identity of ramv to red clover mottle virus (rcmv). | 2005 | 16402684 |
| complete genome sequence of broad bean true mosaic virus. | the complete genome sequence of a severe isolate of broad bean true mosaic virus (genus comovirus, subfamily comovirinae, family secoviridae) is presented. comparison of the amino acid sequences of the capsid proteins and the polymerase showed striking differences to other comoviruses and highest similarities to legume-infecting comoviruses. red clover mottle virus was recognized as the most similar virus with amino acid sequence identities ranging from 43 to 67% for individual genes. | 2010 | 20496090 |
| [cytopathology of plants infected with viruses. ultrastructure of leguminous leaves infected with red clover mottle virus]. | we studied ultrathin sections of clover, pea, and cowpea leaves from intact plants and after infection by red clover mottle virus (rcmv, comovirus, 28 nm isometric virions) under an electron microscope. significant pathological changes were observed in the leaf cells after the infection: rcmv virions accumulated in the cytoplasm and vacuoles, tubular structures containing the virion-like particles, as well as vesicular aggregates were observed. large membrane-containing inclusions with long tubu ... | 2016 | 10881423 |
| some properties of coat proteins of two comoviruses. | properties of coat proteins of red clover mottle virus (rcmv) and broad bean strain virus (bbsv) belonging to comoviruses were studied using polyacrylamide gel electrophoresis in the presence of sds (sds-page), proteolytic cleavage. western blot analysis and monoclonal antibodies (moabs). boiling in the absence of detergent did not cause disintegration of virus particles, but the latter occurred in the presence of 0.2% sds. with 1% sds the disintegration began at 50 degrees c and above 60 degree ... | 1993 | 8105652 |
| the nucleotide sequence of red clover mottle virus bottom component rna. | the complete nucleotide sequence of the bottom component rna (b rna) of red clover mottle virus strain s has been determined. the sequence consists of 6033 nucleotides and contains a single long open reading frame sufficient to encode a protein of m(r) 210,258. the proteolytic processing sites within this protein have been deduced by comparison of its sequence with that of the b rna-encoded protein of cowpea mosaic virus. comparison of the amino acid sequences of the individual proteins confirms ... | 1992 | 1402822 |
| identification and characterization of pseudo-recombinants of red clover mottle comovirus. | cdna clones specific for the two genomic rnas of strain o of the comovirus red clover mottle virus (rcmv) were constructed. using these clones, in conjunction with clones specific for rnas of rcmv strain s, local lesion isolates containing reciprocal pseudo-recombinants between strains s and o were identified. investigation of the biological properties of these pseudo-recombinants showed that the ability of rcmv to infect chenopodium quinoa is determined by b rna. the results also suggest that b ... | 1992 | 1645149 |
| the primary structure of the 24k protease from red clover mottle virus: implications for the mode of action of comovirus proteases. | we have determined the nucleotide sequence of the region of red clover mottle virus (rcmv) bottom component rna which encodes the rcmv equivalent of the cowpea mosaic virus (cpmv) 24k protease. from the alignment of the deduced amino acid sequence of the rcmv 24k protein with that of the homologous protein from cpmv, we speculate on the relative importance of the various amino acid residues which have been implicated in the catalytic mechanism of comovirus proteases. | 1990 | 2179468 |
| identification and subcellular localization of a putative cell-to-cell transport protein from red clover mottle virus. | to investigate the mode of gene expression of red clover mottle virus (rcmv) middle component (m) rna, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the rcmv counterparts of the cowpea mosaic virus (cpmv) 48k and 58k proteins. using an antiserum raised against this synthetic oligopeptide, we have detected a 43-kda protein in the 30,000 g pellet from extracts of rcmv-infected cowpea protoplasts. immunogold cytochemistry further localized this protein to th ... | 1989 | 2596020 |
| loss of infectivity of red clover mottle virus by lysolecithin. | 2-0-hexadecyl-glycero-3-phosphocholine (gpc) 1 mmol/l solution reduced the infectivity of red clover mottle virus (rcmv) by over 90%. the binding of gpc molecules to rcmv particles was investigated with 1h-nmr spectroscopy, which showed a striking interaction between virus particles and gpc molecules. in 10 mmol/l gpc solution each virus particle bound about 10(3) gpc molecules. | 1987 | 2886030 |
| the primary structure of red clover mottle virus middle component rna. | the primary structure of middle component rna of red clover mottle virus (rcmv) has been determined. the rna is polyadenylated, has a small protein (vpg) covalently linked to its 5' terminus and consists of 3543 nucleotides. the rna possesses a single long open reading frame which can code for a polypeptide of molecular weight 109,452. comparison of the 3' terminal sequence of the m rna with that of the homologous b rna reveals a high degree of sequence conservation in this area. computer-assist ... | 1986 | 18640659 |
| serological and electrophoretic typing of red clover mottle virus isolates. | serological and immunoelectrophoretic properties of 22 rcmv isolates from czechoslovakia were determined. based on the differences among the isolates, two electrophoretic types were established: type i--isolates with one non-separable electrophoretic form, and type ii--isolates with two electrophoretic forms. depending on the mobility of the electrophoretic forms these types could be subdivided further into two subtypes each. in all selected isolates representing the individual electrophoretic t ... | 1984 | 6152131 |
| [demonstration of the genome fragmentation of the red clover mottle virus]. | | 2006 | 7382868 |
| the effects of polyacrylic acid and virazole on the replication and component formation of red clover mottle virus. | polyacrylic acid and virazole inhibited the replication of red clover mottle virus (rcmv) in systemically infected pea (pisum sativum) plants, as shown both by serological and infectivity tests. the virazole-induced changes had little effect on the proportion of the three components in the total virus yield, but treatment with polyacrylic acid markedly increased the proportion of the rna-free top component. this suggests that either viral rna synthesis or rna encapsidation was inhibited. | 1979 | 39438 |
| genetic complementation between natural strains of red clover mottle virus. | three strains of red clover mottle virus were purified and their components separated by density gradient and isopycnic centrifugation. the infectivity of the purified components and of homologous and heterologous mixtures of them was assayed using phaseolus vulgaris. infectivity enhancement occurred in all combinations of middle and bottom components. local lesion isolates from heterologous combinations were propagated and their symptoms on pisum sativum and chenopodium quinoa are described. it ... | 1978 | 669903 |
| disintegration of red clover mottle virus virions under different conditions of storage in vitro. | red clover mottle virus was kept in 0.1 m phosphate buffer, ph 7.0, at 4, --20 and --60 degrees c for 1 and 4 weeks and 3 months. the degree of virion disintegration was the highest at --20 degrees c and the lowest at 4 degrees c. two cycles of freezing and thawing destroyed the virions. in frozen samples (at both --20 and --60 degrees c), the nucleoprotein sedimentation component m was disintegrated more than component b. | 1977 | 15440 |
| biological and physiocochemical characteristics of three strains of red clover mottle virus. | | 1976 | 982827 |
| ph stability of red clover mottle virus. | | 1972 | 4403182 |
| photoreactivation of red clover mottle virus. | | 1972 | 4403179 |
| efficient purification procedure for red clover mottle virus. | | 1971 | 4398083 |
| changes in peroxidase isozymes in bean leaves following infection with red clover mottle virus. | | 1970 | 5506588 |
| studies on the effect of temperature on the red clover mottle virus in vivo and in vitro. | | 1969 | 5824396 |
| [on the occurrence of the red clover mottle virus in slovakia]. | | 1966 | 5928917 |
| effects of ultraviolet radiation on infection by intact and phenol-disrupted red clover mottle virus. | | 1961 | 13688062 |