| comoviruses and enteroviruses share a t cell epitope. | an in vitro murine t cell proliferation assay was used to determine whether an antigenic epitope(s) recognized by enterovirus-immune t cells is held in common between plant comoviruses and human enteroviruses. splenocytes isolated from c3h/hej mice infected with coxsackievirus b3 (cvb3) proliferated in vitro not only against a variety of enterovirus (cvb2, cvb3, cvb6, cva16, pv1) antigens, but against comovirus (cpmv, bpmv) antigens as well. splenocytes from mice inoculated with bean pod mottle ... | 1992 | 1370127 |
| nucleotide sequence and genetic map of cowpea severe mosaic virus rna 2 and comparisons with rna 2 of other comoviruses. | we report the nucleotide sequence of cowpea severe mosaic comovirus (cpsmv) genomic rna 2. the molecule is composed of 3732 nucleotide (nt) residues, exclusive of the polyadenylate at the 3' end. only one of the six reading frame registers has a long open reading frame, from nt 255 to nt 3260 in the polarity of encapsidated rna and corresponding to a polyprotein of 1002 amino acid residues (aa). as has been reported for other comoviruses, a second in-frame aug, at nt position 531, apparently als ... | 1992 | 1546463 |
| conformations, interactions, and thermostabilities of rna and proteins in bean pod mottle virus: investigation of solution and crystal structures by laser raman spectroscopy. | we report and interpret laser raman spectra of the three virion components of bean pod mottle virus (bpmv). the top component of bpmv is an empty capsid; middle and bottom components package the rna2 and rna1 genome segments, respectively. all components were investigated as both single crystals and aqueous solutions, the latter over wide ranges of temperature and ionic strength. the isolated rna2 molecule of bpmv middle component was also investigated in both h2o and d2o solutions. the results ... | 1992 | 1637806 |
| influence of the c terminus of the small protein subunit of bean pod mottle virus on the antigenicity of the virus determined using monoclonal antibodies and anti-peptide antiserum. | middle component particles of bean pod mottle virus (bpmv) containing small protein subunits with a cleaved c terminus were used to produce monoclonal antibodies (mabs). all mabs were specific for cryptotopes, i.e. epitopes present only on dissociated bpmv protein. the mabs reacted more strongly with virus protein preparations containing the cleaved form of the small subunit than with preparations containing only the uncleaved form. it seems that the presence of additional residues at the c term ... | 1991 | 1716655 |
| analysis of the nucleotide sequence of bean pod mottle virus middle component rna. | the complete nucleotide sequence of the middle component rna (m rna) of the comovirus bean pod mottle virus (bpmv) has been determined. the sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 da. the proteolytic processing sites within this protein have been identified by comparison with the known three-dimensional structure of the virion and cleavage at these sites would lead to a range of products consistent with those ... | 1991 | 2053290 |
| structural studies of bean pod mottle virus, capsid, and rna in crystal and solution states by laser raman spectroscopy. | structures of protein and rna components of bean pod mottle virus (bpmv) have been investigated by use of laser raman spectroscopy. raman spectra were collected from both aqueous solutions and single crystals of bpmv capsids (top component) and virions (middle and bottom components, which package, respectively, small and large rna molecules). analysis of the data permits the assignment of conformation-sensitive raman bands to viral protein and rna constituents and observation of structural simil ... | 1990 | 2378865 |
| protein-rna interactions in an icosahedral virus at 3.0 a resolution. | nearly 20 percent of the packaged rna in bean-pod mottle virus (bpmv) binds to the capsid interior in a symmetric fashion and is clearly visible in the electron density map. the rna displaying icosahedral symmetry is single-stranded with well-defined polarity and stereochemical properties. interactions with protein are dominated by nonbonding forces with few specific contacts. the tertiary and quaternary structures of the bpmv capsid proteins are similar to those observed in animal picornaviruse ... | 1989 | 2749253 |
| fine structure of plant cells infected with bean pod mottle virus. | | 1972 | 4338989 |
| tubules with viruslike particles in leaf cells infected with bean pod mottle virus. | | 1971 | 5543823 |
| stability differences between the nucleoprotein components of bean pod mottle virus. | | 1965 | 5855568 |
| antigenic analysis of bean pod mottle virus using linear and cyclized synthetic peptides. | the antigenic structure of the comovirus bean pod mottle virus (bpmv) was studied using synthetic peptides selected on the basis of the exposed location of certain regions of the viral protein. three regions of domain a, four regions of domain b and two regions of domain c of bpmv coat protein were studied. each of four regions were synthesized in the form of linear and cyclized peptides while the others were synthesized as linear peptides only. the peptides were tested for their ability to be r ... | 1993 | 7679572 |
| role and mechanism of the maturation cleavage of vp0 in poliovirus assembly: structure of the empty capsid assembly intermediate at 2.9 a resolution. | the crystal structure of the p1/mahoney poliovirus empty capsid has been determined at 2.9 a resolution. the empty capsids differ from mature virions in that they lack the viral rna and have yet to undergo a stabilizing maturation cleavage of vp0 to yield the mature capsid proteins vp4 and vp2. the outer surface and the bulk of the protein shell are very similar to those of the mature virion. the major differences between the 2 structures are focused in a network formed by the n-terminal extensi ... | 1994 | 7849583 |
| raman dynamic probe of hydrogen exchange in bean pod mottle virus: base-specific retardation of exchange in packaged ssrna. | we describe a novel approach to investigating exchange kinetics in biological assemblies. the method makes use of a raman multichannel analyzer coupled with a dialysis flow cell. we employ this methodology to determine exchange rates of labile hydrogens in both the packaged rna genome and protein subunits of bean pod mottle virus (bpmv). in the bpmv assembly, which is similar to human picornaviruses, the x-ray structure indicates that about 20% of the ssrna chain is ordered at the threefold vert ... | 1993 | 8298026 |
| the coat protein genes of squash mosaic virus: cloning, sequence analysis, and expression in tobacco protoplasts. | complementary dna of the middle-component rna of the melon strain of squash mosaic comovirus (sqmv) was cloned. clones containing the coat protein genes were identified by hybridization with a degenerate oligonucleotide synthesized according to the amino acid sequence of a purified peptide fragment of the sqmv large coat protein. a clone containing of 2.5 kbp cdna insert of sqmv m-rna was sequenced. the total insert sequence of 2510 bp included a 2373 bp open reading frame (orf) (encoding 791 am ... | 1993 | 8503782 |
| complete nucleotide sequence of bean pod mottle virus rna1: sequence comparisons and evolutionary relationships to other comoviruses. | the complete nucleotide sequence of bean pod mottle comovirus (bpmv) rna 1 was determined. it is 5983 nucleotides long, excluding the poly(a) tail, and encodes a polyprotein of 1850 amino acid (aa) residues. multiple alignments of the deduced aa sequence of bpmv polyprotein with those of cowpea mosaic virus (cpmv), red clover mottle virus (rcmv) and cowpea severe mosaic virus (cpsmv) indicated that bpmv rna1 encodes the predicted set of five mature proteins: the equivalent of cpmv 32k protease c ... | 1999 | 10403699 |
| studies on hybrid comoviruses reveal the importance of three-dimensional structure for processing of the viral coat proteins and show that the specificity of cleavage is greater in trans than in cis. | a series of cowpea mosaic virus (cpmv)-based hybrid comoviral rna-2 molecules have been constructed. in these, the region encoding both the large (l) and small (s) viral coat proteins was replaced by the equivalent region from bean pod mottle virus (bpmv). the hybrid rna-2 molecules were able to replicate in cowpea protoplasts in the presence of cpmv rna-1. though processing of the hybrid polyproteins by the cpmv-specific 24k proteinase at the site between the 58/48k and l proteins could readily ... | 1999 | 10544093 |
| structural fingerprinting: subgrouping of comoviruses by structural studies of red clover mottle virus to 2.4-a resolution and comparisons with other comoviruses. | red clover mottle virus (rcmv) is a member of the comoviruses, a group of picornavirus-like plant viruses. the x-ray structure of rcmv strain s has been determined and refined to 2.4 a. the overall structure of rcmv is similar to that of two other comoviruses, cowpea mosaic virus (cpmv) and bean pod mottle virus (bpmv). the sequence of the coat proteins of rcmv strain o were modeled into the capsid structure of strain s without causing any distortion, confirming the close resemblance between the ... | 2000 | 10590139 |
| protein-rna interactions and virus stability as probed by the dynamics of tryptophan side chains. | the correlation between dynamics and stability of icosahedral viruses was studied by steady-state and time-resolved fluorescence approaches. we compared the environment and dynamics of tryptophan side chains of empty capsids and ribonucleoprotein particles of two icosahedral viruses from the comovirus group: cowpea mosaic virus (cpmv) and bean pod mottle virus (bpmv). we found a great difference between tryptophan fluorescence emission spectra of the ribonucleoprotein particles and the empty cap ... | 2002 | 12359712 |
| purification and properties of bean pod mottle virus and associated centrifugal and electrophoretic components. | | 1962 | 13864586 |
| evidence for assembly-dependent folding of protein and rna in an icosahedral virus. | ordered nucleic acid in an icosahedral virus was first visualized in the x-ray structure of the picorna-like plant virus, bean pod mottle virus (bpmv). virus particles containing the 3500 nucleotide segment of the bpmv bipartite rna genome (middle component) had nearly 20% of the genome ordered. here we report the refined structures of the middle component, bottom component (particles containing the 5800 nucleotide segment of the genome), and top component (empty particles of bpmv capsid protein ... | 2003 | 14517057 |
| bean leaf beetle (coleoptera: chrysomelidae) management for reduction of bean pod mottle virus. | bean pod mottle virus (bpmv) is a management concern for soybean, glycine max (l.), producers in the north central states because it can cause yield loss and reduce seed quality by induction of seed coat mottling. the main vector of bpmv is the bean leaf beetle, cerotoma trifurcata (forster). an experiment was conducted in 2000 and 2001 at two locations in northwestern and central iowa to test three insecticide treatments for suppression of bean leaf beetles, and subsequently, bpmv. treatments o ... | 2004 | 15154436 |
| surface-exposed c-terminal amino acids of the small coat protein of cowpea mosaic virus are required for suppression of silencing. | the small (s) coat protein of cowpea mosaic virus (cpmv) has been identified previously as a virus-encoded suppressor of post-transcriptional gene silencing (ptgs). deletions within the c-terminal 24 aa of this protein affect the yield and systemic spread of the virus, suggesting that the c-terminal amino acids of the s protein, which are exposed on the surface of assembled virus particles, may be responsible for the suppressor activity. to investigate this, versions of cpmv rna-2 with deletions ... | 2004 | 15483261 |
| the bean pod mottle virus proteinase cofactor and putative helicase are symptom severity determinants. | full-length infectious cdna clones were constructed from the genomic rnas of three distinct strains (k-g7, k-ha1 and k-ho1) of the comovirus bean pod mottle virus (bpmv). whereas k-g7, a subgroup i strain, and k-ha1, a subgroup ii strain produce mild mottling, the reassortant strain k-ho1 (rna1(i) + rna2(ii)) induces necrotic primary lesions on inoculated leaves of soybean and severe systemic leaf mottling and blistering. pseudorecombinants of all possible combinations of transcripts were genera ... | 2005 | 15721361 |
| development of bean pod mottle virus-based vectors for stable protein expression and sequence-specific virus-induced gene silencing in soybean. | plant virus-based vectors provide valuable tools for expression of foreign proteins in plants and for gene function studies. none of the presently available virus vectors is suitable for use in soybean. in the present study, we produced bean pod mottle virus (bpmv)-based vectors that are appropriate for gene expression and virus-induced gene silencing (vigs) in soybean. the genes of interest were inserted into the rna2-encoded polyprotein open reading frame between the movement protein (mp) and ... | 2006 | 16226780 |
| no-choice preference of cerotoma trifurcata (coleoptera: chrysomelidae) to potential host plants of bean pod mottle virus (comoviridae) in iowa. | to better understand the naturally occurring host range of bean pod mottle virus (family comoviridae, genus comovirus, bpmv) and its principal vector cerotoma trifurcata (förster) (coleoptera: chrysomelidae), 18 field-collected perennial plant species were tested for the presence of bpmv. by using no-choice assays, we determined the preference of these plants by bean leaf beetle, by measuring their level of herbivory relative to soybean, glycine max (l.). new food hosts for adult bean leaf beetl ... | 2007 | 17598542 |
| an oleic acid-mediated pathway induces constitutive defense signaling and enhanced resistance to multiple pathogens in soybean. | stearoyl-acyl carrier protein-desaturase (sacpd)-catalyzed synthesis of oleic acid (18:1) is an essential step in fatty acid biosynthesis. arabidopsis mutants (ssi2) with reduced sacpd activity accumulate salicylic acid (sa) and exhibit enhanced resistance to multiple pathogens. we show that reduced levels of 18:1 induce similar defense-related phenotypes in soybean. a bean pod mottle virus (bpmv)-based vector was employed to effectively silence soybean sacpds. the silenced plants contained redu ... | 2008 | 18393616 |
| virus-induced gene silencing in medicago truncatula and lathyrus odorata. | virus-induced gene silencing (vigs) has become an important reverse genetics tool for functional genomics. vigs vectors based on pea early browning virus (pebv, genus tobravirus) and bean pod mottle virus (genus comovirus) are available for the legume species pisum sativum and glycine max, respectively. with the aim of extending the application of the pebv vigs vector to other legumes, we examined susceptibility of 99 accessions representing 24 legume species including 21 accessions of medicago ... | 2008 | 18495283 |
| is preventative, concurrent management of the soybean aphid (hemiptera: aphididae) and bean leaf beetle (coleoptera: chrysomelidae) possible? | in iowa, the management of insect pests in soybean, glycine max (l.) merr., has been complicated by the arrival of the invasive species soybean aphid, aphis glycines matsumura (hemiptera: aphididae), and occasional outbreaks of bean leaf beetle, cerotoma trifurcata (förster) (coleoptera: chrysomelidae), populations leading to economic losses. several insecticide programs designed to reduce abundance of the overwintered and first generation c. trifurcata and the incidence of bean pod mottle virus ... | 2008 | 18613581 |
| the synthesis and processing of the proteins of bean pod mottle virus in rabbit reticulocyte lysates. | translation of the two rna components (b-rna and m-rna) of bean pod mottle virus (bpmv) in rabbit reticulocyte lysates resulted in the formation of a specific set of proteins. among the products formed was a viral protease that catalyzed the cleavage of two precursor proteins coded by m-rna. the protease showed some specificity for bpmv proteins in that it did not cleave the proteins of cowpea mosaic virus (cpmv), which is related to bpmv. conversely, the synthesized bpmv precursor proteins were ... | 1982 | 18638812 |
| evaluation of management strategies for bean leaf beetles (coleoptera: chrysomelidae) and bean pod mottle virus (comoviridae) in soybean. | cerotoma trifurcata förster (coleoptera: chrysomelidae) and bean pod mottle virus (comoviridae) (bpmv) both can reduce yield and seed quality of soybean, glycine max (l.) merr. field experiments were conducted to evaluate the effects of systemic, seed-applied, and foliar-applied insecticides for the management of this pest complex at three locations in central, northeastern, and northwestern iowa during 2002-2004. seed-applied insecticide was evaluated according to a currently recommended manage ... | 2008 | 18767730 |
| novel naturally occurring bean pod mottle virus reassortants with mixed heterologous rna1 genomes. | abstract the comovirus bean pod mottle virus (bpmv) is widespread in the soybean-growing regions in the united states. it has a bipartite genome consisting of rna1 and rna2, which are encapsidated separately. we previously have reported the occurrence in nature of two distinct subgroups of bpmv strains (subgroups i and ii), as well as reassortants between the two subgroups. here, we report the isolation and molecular characterization of naturally occurring partial diploid reassortant strains, wh ... | 2007 | 18942940 |
| diversity among isolates of bean pod mottle virus. | abstract isolates of bean pod mottle virus (bpmv), a member of the genus comovirus, collected from soybean fields in kentucky, virginia, arkansas, and iowa were classified into two distinct subgroups, i and ii, based on nucleic acid hybridization analysis using cloned cdna probes to rna-1 from bpmv strains k-g7 and k-ha1. slot blot hybridization analysis using cloned cdna probes to rna-2 from the same two strains (k-g7 and k-ha1), however, revealed that some of the isolates, initially classified ... | 2002 | 18942958 |
| molecular characterization of naturally occurring rna1 recombinants of the comovirus bean pod mottle virus. | abstract the bean pod mottle virus, a member of the genus comovirus, has a bipartite genome consisting of rna1 and rna2. we previously reported the occurrence in nature of two distinct subgroups of bpmv strains (subgroups i and ii), as well as reassortants between the two subgroups. here, we report on the isolation and molecular characterization of rna1 recombinants from soybean plants infected with the partial diploid reassortant strain il-cb1, which induces very severe symptoms on soybean. cdn ... | 2007 | 18943683 |
| resistance to bean pod mottle virus in transgenic soybean lines expressing the capsid polyprotein. | abstract transgenic fertile soybean plants were generated from somatic embryos of soybean (glycine max) cv. jack transformed via particle bombardment with the capsid polyprotein (pcp) gene of bean pod mottle virus(bpmv). the plant transformation vector (phig/bpmv-pcp) utilized in these experiments contained the bpmv-pcp coding sequence, an intron-containing gus gene, and the hygromycin phosphotransferase gene. southern blot hybridization analysis showed that 19 transgenic soybean plants selected ... | 2001 | 18944228 |
| development and use of an efficient dna-based viral gene silencing vector for soybean. | virus-induced gene silencing (vigs) is increasingly being used as a reverse genetics tool to study functions of specific plant genes. it is especially useful for plants, such as soybean, that are recalcitrant to transformation. previously, bean pod mottle virus (bpmv) was shown to be an effective vigs vector for soybean. however, the reported bpmv vector requires in vitro rna transcription and inoculation, which is not reliable or amenable to high-throughput applications. to increase the efficie ... | 2009 | 19132865 |
| the development of an efficient multipurpose bean pod mottle virus viral vector set for foreign gene expression and rna silencing. | plant viral vectors are valuable tools for heterologous gene expression, and because of virus-induced gene silencing (vigs), they also have important applications as reverse genetics tools for gene function studies. viral vectors are especially useful for plants such as soybean (glycine max) that are recalcitrant to transformation. previously, two generations of bean pod mottle virus (bpmv; genus comovirus) vectors have been developed for overexpressing and silencing genes in soybean. however, t ... | 2010 | 20200069 |
| application of sequence-independent amplification (sia) for the identification of rna viruses in bioenergy crops. | miscanthus x giganteus, energycane, and panicum virgatum (switchgrass) are three potential biomass crops being evaluated for commercial cellulosic ethanol production. viral diseases are potentially significant threats to these crops. therefore, identification of viruses infecting these bioenergy crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. the application is described of sequence-independent amplification, for the identif ... | 2010 | 20638415 |
| silencing genes encoding omega-3 fatty acid desaturase alters seed size and accumulation of bean pod mottle virus in soybean. | omega-3 fatty acid desaturase (fad3)-catalyzed conversion of linoleic acid to linolenic acid (18:3) is an important step for the biosynthesis of fatty acids as well as the phytohormone jasmonic acid (ja) in plants. we report that silencing three microsomal isoforms of gmfad3 enhanced the accumulation of bean pod mottle virus (bpmv) in soybean. the gmfad3-silenced plants also accumulated higher levels of ja, even though they contained slightly reduced levels of 18:3. consequently, the gmfad3-sile ... | 2011 | 21117867 |
| landscape epidemiology of bean pod mottle comovirus: molecular evidence of heterogeneous sources. | bean pod mottle virus (bpmv) rnas are grouped into subgroups (sgi and sgii). a bpmv partial diploid reassortant (ia-di1) from the perennial desmodium illinoense contained both rna1 subgroups and an rna1 recombinant. the rna2 of ia-di1 was characteristic of sgii. additionally, ten bpmv isolates from a soybean field adjacent to the locality of ia-di1 shared >98.5% nucleotide identity with rna1 sgii of ia-di1. the data demonstrate the co-existence of two differing consensus bpmv rna1 subgroups in a ... | 2011 | 21562882 |
| an effective virus-based gene silencing method for functional genomics studies in common bean. | abstract: | 2011 | 21668993 |
| Robust RNAi-based resistance to mixed infection of three viruses in soybean plants expressing separate short hairpins from a single transgene. | Transgenic plants expressing double-stranded RNA (dsRNA) of virus origin have been previously shown to confer resistance to virus infections through the highly conserved RNA-targeting process termed RNA silencing or RNA interference (RNAi). In this study we applied this strategy to soybean plants and achieved robust resistance to multiple viruses with a single dsRNA-expressing transgene. Unlike previous reports that relied on the expression of one long inverted repeat (IR) combining sequences of ... | 2011 | 21999157 |
| soybean homologs of mpk4 negatively regulate defense responses and positively regulate growth and development. | mitogen-activated protein kinase (mapk) cascades play important roles in disease resistance in model plant species such as arabidopsis (arabidopsis thaliana) and tobacco (nicotiana tabacum). however, the importance of mapk signaling pathways in the disease resistance of crops is still largely uninvestigated. to better understand the role of mapk signaling pathways in disease resistance in soybean (glycine max), 13, nine, and 10 genes encoding distinct mapks, mapkks, and mapkkks, respectively, we ... | 2011 | 21878550 |
| Label-free virus detection using silicon photonic microring resonators. | Viruses represent a continual threat to humans through a number of mechanisms, which include disease, bioterrorism, and destruction of both plant and animal food resources. Many contemporary techniques used for the detection of viruses and viral infections suffer from limitations such as the need for extensive sample preparation or the lengthy window between infection and measurable immune response, for serological methods. In order to develop a method that is fast, cost-effective, and features ... | 2012 | 22138465 |
| reduction in fecundity and shifts in cellular processes by a native virus on an invasive insect. | pathogens and their vectors have coevolutionary histories that are intricately intertwined with their ecologies, environments, and genetic interactions. the soybean aphid, aphis glycines, is native to east asia but has quickly become one of the most important aphid pests in soybean-growing regions of north america. in this study, we used bioassays to examine the effects of feeding on soybean infected with a virus it vectors (soybean mosaic virus [smv]) and a virus it does not vector (bean pod mo ... | 2014 | 24682151 |
| recommended reference genes for quantitative pcr analysis in soybean have variable stabilities during diverse biotic stresses. | for real-time reverse transcription-pcr (qrt-pcr) in soybean, reference genes in different tissues, developmental stages, various cultivars, and under stress conditions have been suggested but their usefulness for research on soybean under various biotic stresses occurring in north-central u.s. is not known. here, we investigated the expression stabilities of ten previously recommended reference genes (abct, cyp, ef1a, fbox, gpdh, rpl30, tua4, tub4, tua5, and unk2) in soybean under biotic stress ... | 2015 | 26244340 |
| control of virus diseases in soybeans. | soybean, one of the world's most important sources of animal feed and vegetable oil, can be infected by numerous viruses. however, only a small number of the viruses that can potentially infect soybean are considered as major economic problems to soybean production. therefore, we consider management options available to control diseases caused by eight viruses that cause, or have the potential to cause, significant economic loss to producers. we summarize management tactics in use and suggest di ... | 2014 | 25410106 |
| the pathogenic development of sclerotinia sclerotiorum in soybean requires specific host nadph oxidases. | the plant membrane localized nadph oxidases, also known as respiratory burst oxidase homologs (rbohs), play crucial roles in various cellular activities including plant disease responses, and are a major source of reactive oxygen species (ros). sclerotinia sclerotiorum is a cosmopolitan fungal pathogen that causes sclerotinia stem rot (ssr) in soybean. via a key virulence factor, oxalic acid, it induces programmed cell death (pcd) in the host plant, a process that is reliant on ros generation. i ... | 2017 | 28378935 |
| a sweet story: bean pod mottle virus transmission dynamics by mexican bean beetles (epilachna varivestis). | | 2017 | 28204501 |
| bean pod mottle virus: a new powerful tool for functional genomics studies in pisum sativum. | pea (pisum sativum l.) is an important legume worldwide. the importance of pea in arable rotations and nutritional value for both human and animal consumption have fostered sustained production and different studies to improve agronomic traits of interest. moreover, complete sequencing of the pea genome is currently underway and will lead to the identification of a large number of genes potentially associated with important agronomic traits. because stable genetic transformation is laborious for ... | 2016 | 26896301 |
| shifts in buchnera aphidicola density in soybean aphids (aphis glycines) feeding on virus-infected soybean. | vertically transmitted bacterial symbionts are common in arthropods. aphids undergo an obligate symbiosis with buchnera aphidicola, which provides essential amino acids to its host and contributes directly to nymph growth and reproduction. we previously found that newly adult aphis glycines feeding on soybean infected with the beetle-transmitted bean pod mottle virus (bpmv) had significantly reduced fecundity. we hypothesized that the reduced fecundity was attributable to detrimental impacts of ... | 2015 | 25845267 |
| application of carboxyphenylboronic acid-functionalized magnetic nanoparticles for extracting nucleic acid from seeds. | magnetic iron oxide nanoparticles functionalized with 4-carboxyphenylboronic acid (cpba-mnps) were developed for extracting genomic dna, total rna and nucleic acids from seeds. the seed samples were genetically-modified maize seeds and unmodified soybean seeds infected by bean pod mottle virus and tobacco ringspot virus. the total nucleic acids, genomic dna, and rna could be separately extracted from these seeds with high qualities using cpba-mnps under different conditions. furthermore, the res ... | 2015 | 25214223 |
| the "one-step" bean pod mottle virus (bpmv)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of bpmv r-gene in common bean (phaseolus vulgaris l.). | over the last two years, considerable advances have been made in common bean (phaseolus vulgaris l.) genomics, especially with the completion of the genome sequence and the availability of rnaseq data. however, as common bean is recalcitrant to stable genetic transformation, much work remains to be done for the development of functional genomics tools adapted to large-scale studies. | 2014 | 25168520 |
| positive and negative roles for soybean mpk6 in regulating defense responses. | it has been well established that mpk6 is a positive regulator of defense responses in model plants such as arabidopsis and tobacco. however, the functional importance of soybean mpk6 in disease resistance has not been investigated. here, we showed that silencing of gmmpk6 in soybean using virus-induced gene silencing mediated by bean pod mottle virus (bpmv) caused stunted growth and spontaneous cell death on the leaves, a typical phenotype of activated defense responses. consistent with this ph ... | 2014 | 24762222 |
| disruption of rpp1-mediated soybean rust immunity by virus-induced gene silencing. | phakopsora pachyrhizi, a fungus that causes rust disease on soybean, has potential to impart significant yield loss and disrupt food security and animal feed production. rpp1 is a soybean gene that confers immunity to soybean rust, and it is important to understand how it regulates the soybean defense system and to use this knowledge to protect commercial crops. it was previously discovered that some soybean proteins resembling transcription factors accumulate in the nucleus of rpp1 soybeans. to ... | 2013 | 24401541 |
| the bean pod mottle virus rna2-encoded 58-kilodalton protein p58 is required in cis for rna2 accumulation. | bean pod mottle virus (bpmv) is a bipartite, positive-sense (+) rna plant virus in the secoviridae family. its rna1 encodes proteins required for genome replication, whereas rna2 primarily encodes proteins needed for virion assembly and cell-to-cell movement. however, the function of a 58-kda protein (p58) encoded by rna2 has not been resolved. p58 and the movement protein (mp) of bpmv are two largely identical proteins differing only at their n termini, with p58 extending mp upstream by 102 ami ... | 2014 | 24390330 |
| the 5' untranslated region of bean pod mottle virus rna2 tolerates unusually large deletions or insertions. | bean pod mottle virus (bpmv) is a bipartite, positive-sense (+) rna virus of secoviridae. we recently reported that a 137 nucleotide (nt) stretch (#263-399) of the 466 nt 5' untranslated region (5' utr) of bpmv rna2 can be deleted without compromising bpmv propagation in host plants [lin et al., j. gen. virol. 94 (2013) 1415-1420]. here we demonstrate that nonviral insertions of up to 625 nt is tolerated by the same region. furthermore, one insertion mutant underwent recombination in infected pl ... | 2014 | 24211666 |
| overexpression of gmcam4 in soybean enhances resistance to pathogens and tolerance to salt stress. | plant diseases inflict heavy losses on soybean yield, necessitating an understanding of the molecular mechanisms underlying biotic/abiotic stress responses. ca(2) (+) is an important universal messenger, and protein sensors, prominently calmodulins (cams), recognize cellular changes in ca(2) (+) in response to diverse signals. because the development of stable transgenic soybeans is laborious and time consuming, we used the bean pod mottle virus (bpmv)-based vector for rapid and efficient protei ... | 2014 | 24118726 |
| geospatial and temporal analyses of bean pod mottle virus epidemics in soybean at three spatial scales. | a statewide survey was carried out from 2005 through 2007 to quantify, map, and analyze the spatial dynamics and seasonal patterns of bean pod mottle virus (bpmv) prevalence and incidence within iowa. in all, 8 to 16 soybean fields were arbitrarily sampled from 96 counties in 2005 and all 99 counties in 2006 and 2007. field- and county-scale bpmv prevalence and incidence data were mapped using geographic information systems software. bpmv prevalence was highest in the 2006 soybean growing season ... | 2014 | 24111574 |
| a virus-induced gene silencing method to study soybean cyst nematode parasitism in glycine max. | bean pod mottle virus (bpmv) based virus-induced gene silencing (vigs) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. however, bpmv-vigs protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed. | 2013 | 23830484 |
| a stem-loop structure in the 5' untranslated region of bean pod mottle virus rna2 is specifically required for rna2 accumulation. | bean pod mottle virus (bpmv) is a bipartite, positive-sense (+) rna plant virus of the family secoviridae. its rna1 encodes all proteins needed for genome replication and is capable of autonomous replication. by contrast, bpmv rna2 must utilize rna1-encoded proteins for replication. here, we sought to identify rna elements in rna2 required for its replication. the exchange of 5' untranslated regions (utrs) between genome segments revealed an rna2-specific element in its 5' utr. further mapping l ... | 2013 | 23388202 |
| virus-induced gene silencing in soybean and common bean. | plant viral vectors are useful for transient gene expression as well as for downregulation of gene expression via virus-induced gene silencing (vigs). when used in reverse genetics approaches, vigs offers a convenient way of transforming genomic information into knowledge of gene function. efforts to develop and improve plant viral vectors have expanded their applications and have led to substantial advances needed to facilitate gene function studies in major row crops. here, we describe a dna-b ... | 2013 | 23386301 |
| one-step detection of bean pod mottle virus in soybean seeds by the reverse-transcription loop-mediated isothermal amplification. | bean pod mottle virus (bpmv) is a wide-spread and destructive virus that causes huge economic losses in many countries every year. a sensitive, reliable and specific method for rapid surveillance is urgently needed to prevent further spread of bpmv. | 2012 | 22958497 |
| temporal and spatial bean pod mottle virus-induced gene silencing in soybean. | virus-induced gene silencing (vigs) is a powerful reverse genetics tool in plant science. in this study, we investigated the temporal and spatial silencing patterns achieved by bean pod mottle virus (bpmv)-based vigs in soybean using virus constructs targeting green fluorescence protein (gfp). silencing gfp enabled an in-depth analysis of silencing in soybean tissues over time in a transgenic line constitutively expressing gfp. we discovered evidence for variable gfp silencing based on insert or ... | 2012 | 22738403 |
| virus-induced gene silencing in soybean. | understanding the biology of a system requires the association of gene functions to phenotypes and vice versa. although a large number of resources and genomic tools are available for studies in the crop plant soybean, investigations related to gene function have been lacking. this is largely due to the fact that rapid functional genomics tools have been unavailable for application in soybean. this constraint was recently alleviated when a novel bean pod mottle virus (bpmv)-based system for viru ... | 2012 | 22678587 |
| detection of members of the secoviridae in the tallgrass prairie preserve, osage county, oklahoma, usa. | viruses are most frequently discovered because they cause disease. to expand knowledge of plant-associated viruses beyond these narrow constraints, non-cultivated plants of the tallgrass prairie of the united states were systematically surveyed for evidence of viruses. this report discusses putative viruses of the family secoviridae identified by the survey. sequence analysis suggests the presence of at least six viruses in the study site, including bean pod mottle virus, maize chlorotic dwarf v ... | 2012 | 22487310 |
| production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene. | soybean (glycine max. merrill. cv. fayette) cotyledonary nodes were transformed with bean pod mottle virus (bpmv) coat protein precursor (cp-p) gene via agrobacterium-mediated transformation. the transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. southern blot hybridization verified the integration of the bpmv cp-p gene. inheritance and expression of this gene in r1 plants were also demonstrated. abo ... | 1996 | 24178163 |
| robust rnai-mediated resistance to infection of seven potyvirids in soybean expressing an intron hairpin nib rna. | viral pathogens, such as soybean mosaic virus (smv), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. engineering resistance by rnai-mediated gene silencing is a powerful strategy for controlling viral diseases. in this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, nib) gene was isolated from the smv sc3 strain, driven by the leaf-specific rbcs2 promoter from phaseolus vulgaris, and introduced into soybean. th ... | 2017 | 28840434 |
| rnai-mediated smv p3 cistron silencing confers significantly enhanced resistance to multiple potyvirus strains and isolates in transgenic soybean. | robust rnai-mediated resistance to multiple potyvirus strains and isolates, but not to secovirus bpmv, was conferred by expressing a short smv p3 hairpin in soybean plants. engineering resistance to multiple potyvirus strains is of great interest because of a wide variability of the virus strains, and mixed infections of multiple viruses or strains commonly associated with field grown soybean. in this study, rnai-mediated silencing of the soybean mosaic virus (smv) p3 cistron, which is reported ... | 2017 | 28756582 |
| protection against common bean rust conferred by a gene-silencing method. | rust disease of the dry bean plant, phaseolus vulgaris, is caused by the fungus uromyces appendiculatus. the fungus acquires its nutrients and energy from bean leaves using a specialized cell structure, the haustorium, through which it secretes effector proteins that contribute to pathogenicity by defeating the plant immune system. candidate effectors have been identified by dna sequencing and motif analysis, and some candidates have been observed in infected leaves by mass spectrometry. to asse ... | 2017 | 28437139 |
| virus-induced gene silencing (vigs) and foreign gene expression in pisum sativum l. using the "one-step" bean pod mottle virus (bpmv) viral vector. | plant viral vectors have been developed to facilitate gene function studies especially in plant species not amenable to traditional mutational or transgenic modifications. in the fabaceae plant family, the most widely used viral vector is derived from bean pod mottle virus (bpmv). originally developed for overexpression of foreign proteins and vigs studies in soybean, we adapted the bpmv-derived vector for use in other legume species such as phaseolus vulgaris and pisum sativum. here, we describ ... | 2017 | 28986801 |
| rapid detection of fifteen known soybean viruses by dot-immunobinding assay. | a dot-immunobinding assay (diba) was optimized and used successfully for the rapid detection of 15 known viruses [alfalfa mosaic virus (amv), bean pod mottle virus (bpmv), bean yellow mosaic virus (bymv), cowpea mild mottle virus (cpmmv), cowpea severe mosaic virus (cpsmv), cucumber mosaic virus (cmv), peanut mottle virus (pemov), peanut stunt virus (psv), southern bean mosaic virus (sbmv), soybean dwarf virus (sbdv), soybean mosaic virus (smv), soybean vein necrosis virus (svnv), tobacco ringsp ... | 2017 | 28887190 |