| coat protein rnas-mediated protection against andean potato mottle virus in transgenic tobacco. | the expression of translatable sequences of either one of the two andean potato mottle virus (apmov) coat protein (cp) genes (cp22 and cp42) and of the nontranslatable sequence of cp42 in transgenic tobacco provided protection against apmov. resistance was mediated by cp transgene rnas rather than the protein, as an inverse correlation between resistance and the accumulation levels of cps transgene mrnas was observed. these data indicated that a post-transcriptional gene silencing (ptgs) mechani ... | 2001 | 11448745 |
| the 18s rdna sequence of synchytrium endobioticum and its utility in microarrays for the simultaneous detection of fungal and viral pathogens of potato. | resting spores extracted from wart (synchytrium endobioticum)-infected potato tubers were used for dna extraction and amplification of 18s rdna. analysis of the cloned, sequenced fragment revealed high similarity to members of the chytridiomycota. using this information, specific oligonucleotide probes were designed and arrayed onto glass slides for detection of the pathogen. viral sequence information available in the databank was retrieved, or new viral sequences were generated, and used to de ... | 2005 | 15800764 |
| molecular cloning and sequence analysis of a segment from andean potato mottle virus b rna encoding the putative rna polymerase. | andean potato mottle virus (apmv), an endemic south american comovirus, has a bipartite genome consisting of two plus-strand rna molecules (m and b rna). we have cloned the 3' half of the b rna and identified the complete sequence of the putative apmv rna polymerase. the rna polymerase gene is part of a large polyprotein-encoding open reading frame. the putative, mature rna polymerase, as deduced by comparison with the related cowpea mosaic virus (cpmv), type member of the comovirus group, is 70 ... | 1993 | 8429307 |
| nucleotide sequence analysis of an andean potato mottle virus middle component rna cdna clone: comparisons of the encoded proteins with those of other comoviruses. | andean potato mottle virus (apmv) is a comovirus whose genomic structure consists of two plus-strand rna molecules (m- and b-rna). here we report the nucleotide sequence analysis of an apmv m-rna cdna clone with 3,669 nucleotide (nt) residues, exclusive of the polyadenylate at the 3' end, covering approximately 99% of the apmv m-rna. the first initiation codon in register translates from nt 194 to 3185 a polyprotein of 997 amino acid (aa) residues. a second initiation codon in register, beginnin ... | 1993 | 8150598 |
| complete nucleotide sequence of the 22 kda coat protein of andean potato mottle virus. | | 1992 | 1623195 |
| molecular cloning and sequence analysis of an andean potato mottle virus cdna clone encoding the major coat protein. | | 1992 | 16652998 |
| the 42-kda coat protein of andean potato mottle virus acts as a transcriptional activator in yeast. | interactions of viral proteins play an important role in the virus life cycle, especially in capsid assembly. andean potato mottle comovirus (apmov) is a plant rna virus with a virion formed by two coat proteins (cp42 and cp22). both apmov coat protein open reading frames were cloned into pgbt9 and pgad10, two-hybrid system vectors. hf7c yeast cells transformed with the p9cp42 construct grew on yeast dropout selection media lacking tryptophan and histidine. clones also exhibited beta-galactosida ... | 2002 | 11960188 |
| co-suppression mediated virus resistance in transgenic tobacco plants harboring the 3'-untranslated region of andean potato mottle virus. | nicotiana tabacum plants were transformed with the 3'-untranslated region of the andean potato mottle virus (apmov) genome rna-2. three strategies were used: the introduction of this region in sense and in antisense orientation and of a fragment comprising the entire 3'-untranslated region from rna-2 and part of the cp22 coat protein sequence. the transgenic lines were inoculated with the virus and different responses were observed, ranging from susceptibility to apmov to complete immunity to vi ... | 2001 | 11817537 |