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purification of filamentous plant viruses by thin layer centrifugation.the construction of a modified thin layer ultracentrifuge rotor is described. this rotor was used in the purification of five filamentous plant viruses, viz. tmv, scmv, pvx, sgv and ymc. the purification and concentration of these viruses in their monomeric forms is hazardous when conventional "tube" rotors are used since they invariably result in dissociation and aggregation of the virus particles. using the thin layer rotor these infective agents may be concentrated in volumes of fluid equal t ...19751178651
nucleotide sequence of the 3'-terminal region of potato virus a rna.the sequence of the 3'-terminal region of the genome of the potato virus a (pva) was obtained from two independent cdna clones. this sequence is 1383 nucleotides long and contains an open reading frame of 1178 nucleotides, ending with the translation termination codon taa and followed by untranslated region of 205 nucleotides. since the n-terminal amino acid of the coat protein of pva was blocked, the position of the putative coat protein cleavage site has been deduced by searching for consensus ...19921604933
transient expression of the coat protein of sugarcane mosaic virus in sugarcane protoplasts and expression in escherichia coli.the coat protein (cp) of strain sc of sugarcane mosaic virus (scmv-sc) was expressed transiently in sugarcane protoplasts after electroporation with one of two plasmids encoding the cp gene. the cp gene was fused with either the cauliflower mosaic virus 35s promoter or the synthetic monocotyledon promoter "emu". the coat protein gene was also inducibly expressed in escherichia coli when fused to the trc promoter. the protein expressed in both systems had the same electrophoretic mobility and ant ...19921642548
the palindromic series i repeats in the simian cytomegalovirus major immediate-early promoter behave as both strong basal enhancers and cyclic amp response elements.a 600-base-pair (bp) enhancer region upstream from the major ie94 gene of simian cytomegalovirus (scmv) produces very strong basal expression of associated gene products. this domain consists of multiple sets of interspersed repetitive elements, including 11 copies of a conserved 16-bp palindromic sequence with the consensus ccattgacgtcaatgg. these series i repeats contain an 8-bp core tgacgtca that resembles the cyclic amp (camp) response element (cre) of cellular genes. in transient chloramphe ...19902152815
nucleotide sequence of the 3'-terminal region of potato virus yn rna.the sequence of the 3'-terminal 1611 nucleotides of the genome of the tobacco veinal necrosis strain of potato virus y (pvyn) was determined. the sequence revealed an open reading frame of 1285 nucleotides, of which the start was not identified, and an untranslated region of 316 nucleotides upstream of a poly(a) tract. comparison of the open reading frame with the amino-terminal sequence of the viral coat protein enabled mapping of the start of the coat protein at amino acid -267, and indicated ...19892732687
location, transcript analysis, and partial nucleotide sequence of the cytomegalovirus gene encoding an early dna-binding protein with similarities to icp8 of herpes simplex virus type 1.the results presented here locate the gene encoding an early, nonvirion, single-stranded dna-binding protein of human and simian strains of cytomegalovirus (cmv) [hcmv(towne) db140 and scmv(colburn) db129, respectively] and provide additional evidence that this protein is the cmv homolog of the herpes simplex virus type 1 (hsv-1) major dna-binding protein (icp8), as proposed earlier (d. g. anders, a. irmiere, and w. gibson, j. virol. 58:253-262). the icp8 gene was used as a probe in southern ana ...19882831398
multiple tandemly repeated binding sites for cellular nuclear factor 1 that surround the major immediate-early promoters of simian and human cytomegalovirus.we show that the large dna genomes of human and simian cytomegaloviruses (hcmv and scmv, respectively) each contain multiple binding sites for purified cellular nuclear factor 1 (nf1) protein. examination of the major immediate-early (ie) gene region in the hindiii h fragment of scmv (colburn) by filter binding assays showed that it competed 45-fold better than the single adenovirus type 2 binding site for nf1 protein and that it contained at least two distinct binding loci. direct dnase i footp ...19873033283
coat protein of potyviruses. 5. symptomatology, serology, and coat protein sequences of three strains of passionfruit woodiness virus.three strains of passionfruit woodiness virus, tip blight (pwv-tb), severe (pwv-s) and mild (pwv-m), were compared on the basis of their biological, serological and coat protein structural properties. each of the strains could be distinguished on the basis of their reactions on selected test plant species but no differences were observed in the serological properties of the three pwv strains. molecular weight estimates on sds-page suggest the pwv coat protein contains 275 amino acid residues and ...19883202695
[influence of ph on the sugarcane mosaic virus]. 19714261514
catalase and peroxidase activity in sugarcane infected with sugarcane mosaic virus. 19705413808
purification and electron microscopy of sugarcane mosaic virus. 19665905297
protein counterparts of human and simian cytomegaloviruses.cytomegalovirus (cmv) proteins from isolates of both human (hcmv) and simian (scmv) origin have been compared. three classes were analyzed: the immediate-early (ie) proteins, other infected-cell-specific proteins not present in virus particles, and the proteins that constitute the mature extracellular virion. comparisons were based on one- and two-dimensional (charge-size) separations in denaturing polyacrylamide gels, and on the selectivity of biosynthetic radiolabeling with [32p]orthophosphate ...19836310864
further studies on the use of protein a in immune electron microscopy for detecting virus particles.the immune electron microscopic technique which involves pre-coating electron microscope grids with protein a before coating them with the specific antiserum, has been found suitable for detecting isometric insect and plant viruses. with the three virus-antibody combinations tested, the optimum antiserum dilution for protein a plus antiserum treatment was found to be 1:100 or less, whereas in the case of grids treated with antiserum alone it ranged from 1:1000 to 1:2000 although the titre of the ...19807014777
electro-blot radioimmunoassay of virus-infected plant sap - a powerful new technique for detecting plant viruses.a new technique for detecting viruses in plant sap is described. it consists of sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the infected plant sap, electrophoretic transfer of protein bands to activated paper by the electro-blot technique, the subsequent probing of the viral coat protein band by specific antiserum (prepared against intact virus), and detection of immune complex with 125 i-labelled protein a. the technique successfully detected tobacco mosaic virus at a sap dilu ...19827061671
expression, purification, and use as an antigen of recombinant sugarcane mosaic virus coat protein.a high titre (1:10,000) antiserum was raised in a rabbit against the coat protein of sugarcane mosaic potyvirus (scmv), by injecting a preparation of recombinant coat protein purified from a fusion protein expressed in e. coli. the fusion protein consisted of the male maltose binding protein (mbp) and the viral coat protein separated by the protease factor xa cleavage site. the fusion protein was encoded by the plasmid pmal-ccpm, which was constructed by cloning a modified coat protein gene to t ...19957503681
human cytomegalovirus proteinase: candidate glutamic acid identified as third member of putative active-site triad.the human cytomegalovirus (hcmv) proteinase is synthesized as a 709-amino-acid precursor that undergoes at least three autoproteolytic cleavages. the mature proteinase, called assemblin, is one of the products of autoproteolysis and is composed of the first 256 amino acids of the precursor. hcmv assemblin and its homologs in other herpes group viruses contain five highly conserved domains (cd1 through cd5). an absolutely conserved serine in cd3 has been shown by site-directed mutagenesis of the ...19957769716
cytomegalovirus protein substrates are not cleaved by the herpes simplex virus type 1 proteinase.the herpesvirus maturational proteinase, assemblin, is made as a precursor that undergoes at least two autoproteolytic cleavages--one in a sequence toward its carboxyl end, called the maturational (m) site, and one in a sequence toward its midpoint, called the release (r) site. the m- and r-site sequences are both well conserved among the herpesvirus proteinase homologs, suggesting that the proteinase of one herpesvirus might be able to cleave the substrates of another. to test this possibility, ...19957983728
comparison of the czech scrophularia isolate with the italian anagyris strain of scrophularia mottle virus.scrophularia mottle virus (scmv) was newly found in the czech republic in scrophularia nodosa l. plants. the czech isolate (scmv-c) was serologically identical and similar in symptoms and host range to the italian anagyris strain (scmv-i). nicotiana tabacum l. cv. samsun, nicotiana glutinosa l., nicotiana tabacum l. cv. white burley, physalis floridana rybd. and cucumis sativus l. are described as new host plants of scmv. double-stranded rna patterns and the isoelectric point of this virus are c ...19948067309
sequence diversity in the surface-exposed amino-terminal region of the coat proteins of seven strains of sugarcane mosaic virus correlates with their host range.the n-terminal region of the coat proteins of five strains (isis, brisbane, sabi, bundaberg, and bc) of sugarcane mosaic virus (scmv) isolated from four different plant species (sugarcane, sabi grass, wild sorghum, and blue couch grass) have been compared with the previously published data for scmv-sc and scmv-mdb, isolated from sugarcane and maize, respectively. the region, beginning at residue 11 and ending 16 residues beyond the second trypsin cleavage site of the coat protein, varied in size ...19938379856
identification of a large bent dna domain and binding sites for serum response factor adjacent to the nfi repeat cluster and enhancer region in the major ie94 promoter from simian cytomegalovirus.the major immediate-early (mie) transactivator proteins of cytomegaloviruses (cmv) play a pivotal role in the initiation of virus-host cell interactions. therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of the outcome of virus infection. s1 nuclease analysis and in vitro transcription assays with the mie (or ie94) transcription unit of simian cmv (scmv) (colburn) revealed a single pro ...19938380090
monitoring fusion protein cleavage by capillary electrophoresis.capillary electrophoresis was successfully utilized to monitor the proteolytic cleavage of two fusion proteins; maltose binding protein-sugarcane mosaic potyvirus coat protein and maltose binding protein-paramyosin. the course of proteolysis by factor xa was monitored with high sensitivity and virtually in real time. capillary electrophoresis conditions were optimized to clearly resolve the fusion proteins, factor xa, lysis products and buffer components. rapid elucidation of time to complete di ...19938424876
simian cytomegalovirus-related stealth virus isolated from the cerebrospinal fluid of a patient with bipolar psychosis and acute encephalopathy.a cytopathic 'stealth' virus was cultured from the cerebrospinal fluid of a patient with a bipolar psychotic disorder who developed a severe encephalopathy leading to a vegetative state. dna sequencing of a polymerase chain reaction-amplified product from infected cultures has identified the virus as an african green monkey simian cytomegalovirus (scmv)-related stealth virus. the virus is similar to the scmv-related stealth virus isolated from a patient with chronic fatigue syndrome. the finding ...19968888270
cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus ul46.capsids of human and simian strains of cytomegalovirus (hcmv and scmv, respectively) have identified counterparts for all but one of the protein components of herpes simplex virus (hsv) capsids. the open reading frames (orfs) for the cmv and hsv counterpart proteins are positionally homologous in the two genomes. the hsv capsid protein without a recognized counterpart in cmv is vp19c, a 50-kda element of the intercapsomeric "triplex." vp19c is encoded by hsv orf ul38, whose positional homolog in ...19968892863
synergistic interactions between overlapping binding sites for the serum response factor and elk-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus major immediate-early promoters in monocyte and t-lymphocyte cell types.cytomegalovirus (cmv) infection is nonpermissive or persistent in many lymphoid and myeloid cell types but can be activated in differentiated macrophages. we have shown elsewhere that both the major immediate-early gene (mie) and lytic cycle infectious progeny virus expression can be induced in otherwise nonpermissive monocyte-like u-937 cell cultures infected with either human cmv (hcmv) or simian cmv (scmv) by treatment with the phorbol ester 12-o-tetradecanoylphorbol-13-acetate (tpa). two mul ...19968970984
sequence diversity in the coat protein coding region of twelve sugarcane mosaic potyvirus isolates from australia, usa and south africa.we have sequenced the coat protein (cp) coding region of 11 field isolates of scmv from australia, usa and south africa. the differences between the nucleotide sequences of the isolates was 0.2 to 4.1% and the encoded amino acid sequences differed by 0.0 to 3.5%. phylogenetic analysis of the cp coding sequences of the scmv isolates and the related potyviruses scmv-mdb, jgmv, srmv, mdmv-a and pvy showed that the scmv isolates formed a tightly clustered group, with scmv-mdb forming a separate bran ...19989687871
capsid structure of simian cytomegalovirus from cryoelectron microscopy: evidence for tegument attachment sites.we have used cryoelectron microscopy and image reconstruction to study b-capsids recovered from both the nuclear and the cytoplasmic fractions of cells infected with simian cytomegalovirus (scmv). scmv, a representative betaherpesvirus, could thus be compared with the previously described b-capsids of the alphaherpesviruses, herpes simplex virus type 1 (hsv-1) and equine herpesvirus 1 (ehv-1), and of channel catfish virus, an evolutionarily remote herpesvirus. nuclear b-capsid architecture is ge ...19999971801
high-resolution mapping of loci conferring resistance to sugarcane mosaic virus in maize using rflp, ssr, and aflp markers.sugarcane mosaic virus (scmv) is one of the most important virus diseases of maize in europe. genetic analysis on backcross five (bc5) progeny derived from the cross fap1360a (resistant) x f7 (susceptible) confirmed that at least two dominant genes, scm1 and scm2, are required for resistance to scmv in the progeny of this cross. with the aid of rflp and ssr marker analyses, scm1 was mapped in the region of 8.7 cm between the nucleolus organizer region (nor) and rflp marker bnl6.29 on the short a ...199910323240
melanoma growth stimulatory activity (mgsa/gro-alpha) chemokine genes incorporated into an african green monkey simian cytomegalovirus-derived stealth virus.dna isolated from the culture of an african green monkey simian cytomegalovirus (scmv)-derived stealth virus has been cloned. a region of the virus that contains genes coding proteins homologous to the ul141, ul144, and ul145 proteins of human cytomegalovirus has recombined with cellular sequences encoding several distinct copies of the melanoma growth stimulatory activity (mgsa/gro-alpha) chemokine gene. this finding illustrates the capacity of stealth viruses to capture, amplify, and mutate ge ...199910331960
molecular characterization of a new potyvirus infecting sunflower.we have sequenced 1873 nucleotides from the 3'-end region of a sunflower potyvirus genome including the 3'-nib protein coding region (813 nucleotides), the entire coat protein coding region (807 nucleotides) and 3'-ncr (253 nucleotides), excluding the poly (a) tail. amino acids identity of the whole cp between the sunflower virus and potyvirus members ranged from 49.5% (scmv) to 81.5% (pvy-nsnr), and the core ranged from 55% (tvmv) to 87% (pvy-nsnr; pepmov). the 3'-ncr nucleotides showed 38.7% h ...200011205115
maize dwarf mosaic disease in different regions of china is caused by sugarcane mosaic virus.sugarcane mosaic virus (scmv) was detected in all 62 maize samples collected from eight maize-growing provinces in china showing dwarf mosaic symptoms by immunocapture reverse-transcription polymerase chain reaction (rt-pcr). maize dwarf mosaic virus (mdmv), sorghum mosaic virus (srmv) and johnsongrass mosaic virus (jgmv), however, were not detected in any of the samples by rt-pcr. eleven cdna fragments of approximately 0.8 kilobases covering most of the coat protein (cp) gene of scmv were seque ...200212491109
development of rga-caps markers and genetic mapping of candidate genes for sugarcane mosaic virus resistance in maize.three previously published resistance gene analogues (rgas), pic13, pic21 and pic19, were mapped in relation to sugarcane mosaic virus (scmv) resistance genes ( scmv1, scmv2) in maize. we cloned these rgas from six inbreds including three scmv-resistant lines (d21, d32, fap1360a) and three scmv-susceptible lines (d145, d408, f7). pairwise sequence alignments among the six inbreds revealed a frequency of one single nucleotide polymorphism (snp) per 33 bp for the three rgas, indicating a high degr ...200212582538
saturation of two chromosome regions conferring resistance to scmv with ssr and aflp markers by targeted bsa.quantitative trait loci (qtls) and bulked segregant analyses (bsa) identified the major genes scmv1 on chromosome 6 and scmv2 on chromosome 3, conferring resistance against sugarcane mosaic virus (scmv) in maize. both chromosome regions were further enriched for ssr and aflp markers by targeted bulked segregant analysis (tbsa) in order to identify and map only markers closely linked to either scmv1 or scmv2. for identification of markers closely linked to the target genes, symptomless individual ...200312589549
simian cytomegalovirus and contamination of oral poliovirus vaccines.in the 1950s the use of primary rhesus macaque kidney cultures to propagate poliovirus for vaccine production led to the contamination of vaccines with simian virus 40 (sv40). african green monkey kidney (agmk) cultures free of sv40 were used as an alternative cell substrate for vaccine manufacture. in this study we evaluate oral poliovirus seeds, vaccine bulks and vaccines themselves for the presence of a common contaminant of agmk cultures, simian cytomegalovirus (scmv). using sensitive polyme ...200312623061
complete sequence of the genomic rna of the prevalent strain of a potyvirus infecting maize in china.the complete nucleotide sequence of the prevalent strain of a potyvirus isolated from maize in beijing, china was determined and compared with other closely related potyviruses. the viral genome comprises 9595 nucleotides, excluding the poly (a) tail, and encodes a putative polyprotein of 3063 amino acid residues. sequence comparison of the coat proteins showed that this isolate was most closely related to most other potyviral isolates infecting maize across china with identities of about 99% an ...200312664299
identification of genetically linked rgas by bac screening in maize and implications for gene cloning, mapping and mas.the resistance gene analogue (rga) pic19 in maize, a candidate for sugarcane mosaic virus (scmv) resistance gene (r gene) scmv1, was used to screen a maize bac library to identify homologous sequences in the maize genome and to investigate their genomic organisation. fifteen positive bac clones were identified and could be classified into five physically independent contigs consisting of overlapping clones. genetic mapping clustered three contigs into the same genomic region as scmv1 on chromoso ...200312748766
simian cytomegalovirus encodes five rapidly evolving chemokine receptor homologues.many herpesviruses, poxviruses and retroviruses encode proteins related to chemokines and chemokine receptors. the first one discovered, us28 of human cytomegalovirus (hcmv), is a 7-transmembrane domain g protein-coupled chemokine receptor able to activate diverse cellular responses, including cell migration and gene expression. a related orf named us27 is adjacent to us28, but no functions have been defined yet. recently orfs 3-7, a cluster of five concatenated orfs with highest homology to us2 ...200414739653
african green monkey origin of the atypical cytopathic 'stealth virus' isolated from a patient with chronic fatigue syndrome.a cytomegalovirus-like 'stealth virus' had previously been isolated from a patient with the chronic fatigue syndrome (cfs).199515566831
identification of a naturally occurring recombinant isolate of sugarcane mosaic virus causing maize dwarf mosaic disease.the complete nucleotide sequence of a potyvirus causing severe maize dwarf mosaic disease in shaanxi province, northwestern china was determined (genbank accession no. ay569692). the full genome is 9596 nucleotides in length excluding the 3 '-terminal poly (a) sequence. it contains a large open reading frame (orf) flanked by a 149 nt 5'-untranslated region (utr) and a 255 nt 3'-utr. the putative polyprotein encoded by this large orf comprises of 3063 amino acid residues. sequence comparisons and ...200515744565
identification by suppression subtractive hybridization of genes that are differentially expressed between near-isogenic maize lines in association with sugarcane mosaic virus resistance.the molecular mechanisms underlying the development and progression of sugarcane mosaic virus (scmv) infection in maize are poorly understood. a study of differential expression was conducted to identify genes involved in resistance to scmv. in this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differently expressed in the near isogenic lines f7+ (scmv resistant) and f7 (susceptible). altogether, 302 differentially expressed gene ...200515891912
cloning and sequence analysis of coat protein gene for characterization of sugarcane mosaic virus isolated from sugarcane and maize in thailand.three isolates of sugarcane mosaic virus (scmv) from sugarcane and maize in thailand, were selected and used as viral sources for coat protein (cp) genes cloning by immunocapture reverse transcription polymerase chain reaction (ic-rt-pcr). the cp gene sequences and their deduced amino acids were determined. the sequences of three cloned cp genes, ut6th-sgc and ud7th-sgc from sugarcane, and sbc2th-mz from maize were compared with other previously reported sequences of scmv-cp gene and their phylo ...200616195785
coat protein gene sequence of a mexican isolate of sugarcane mosaic virus and its infectivity in maize and sugarcane plants. 200616328150
prospecting the utility of a pmi/mannose selection system for the recovery of transgenic sugarcane (saccharum spp. hybrid) plants.for the first time, the phosphomannose isomerase (pmi, ec 5.3.1.8)/mannose-based "positive" selection system has been used to obtain genetically engineered sugarcane (saccharum spp. hybrid var. cp72-2086) plants. transgenic lines of sugarcane were obtained following biolistic transformation of embryogenic callus with an untranslatable sugarcane mosaic virus (scmv) strain e coat protein (cp) gene and the escherichia coli pmi gene mana, as the selectable marker gene. postbombardment, transgenic ca ...200717149641
analysis of sugarcane mosaic virus resistance in maize in an isogenic dihybrid crossing scheme and implications for breeding potyvirus-resistant maize hybrids.the gene action of 2 sugarcane mosaic virus (scmv) resistance loci in maize, scmv1 and scmv2, was evaluated for potyvirus resistance in an isogenic background. all 4 homozygous and 5 heterozygous isogenic genotypes were produced for introgressions of the resistant donor (fap1360a) alleles at both loci into the susceptible parent (f7) genetic background using simple sequence repeat markers. for scmv and maize dwarf mosaic virus (mdmv), virus symptoms appeared rapidly in the 3 homozygous genotypes ...200617213909
complete genome sequences of maize dwarf mosaic and sugarcane mosaic virus isolates coinfecting maize in spain.the genomes of spanish isolates of maize dwarf mosaic virus (mdmv-sp) and sugarcane mosaic virus (scmv-sp) were completely sequenced. nucleotide sequence identities of scmv-sp to those of other scmv isolates ranged from 79 to 90%. mdmv-sp shared 85% nucleotide identity with the only other fully sequenced isolate of mdmv. mdmv-sp and scmv-sp differed from each other by 31% in their nucleotide sequences. phylogenetic analyses showed that scmv isolates group by host rather than by geographical loca ...200717680319
[comparative qtl mapping of resistance to sugarcane mosaic virus in maize based on bioinformatics].the development of genomics and bioinformatics offers new tools for comparative gene mapping. in this paper, an integrated qtl map for sugarcane mosaic virus (scmv) resistance in maize was constructed by compiling a total of 81 qtl loci available with the genetic map ibm2 2005 neighbors as reference. these 81 qtl loci were scattered on 7 chromosomes of maize, and most of them was clustered on chromosome 3 and 6. by using meta- analysis method, we identified one and two "consensus qtls" on chromo ...200818244910
contrasting effects of hc-pro and 2b viral suppressors from sugarcane mosaic virus and tomato aspermy cucumovirus on the accumulation of sirnas.rna silencing and suppression of silencing are host and virus interactions for defense and counter-defense. here, we explored the function effect of hc-pro encoded by sugarcane mosaic virus (scmv) on the suppression of rna silencing. sirna northern blotting assay indicated that the replication of scmv was regulated by host rna silencing machinery. co-expression assay demonstrated that the hc-pro encoded by scmv suppressed the rna silencing induced by sense rna and dsrna. transitive rna silencing ...200818280529
the genomic sequence and biological properties of pennisetum mosaic virus, a novel monocot-infecting potyvirus.the complete nucleotide sequences of two isolates of pennisetum mosaic virus (penmv) were determined. the viral genome comprised 9,611 nucleotides (nt) excluding the 3'-terminal poly(a) sequence, with the capacity of encoding a single polyprotein of 3,065 amino acids. the large open reading frame is flanked by a 172-nt 5'-untranslated region (utr) and a 244-nt 3'-utr. sequence comparisons and phylogenetic analyses of the complete genome and polyproteins suggest that penmv is closely related to o ...200818365128
viruses causing mosaic disease in sugarcane and their genetic diversity in southern china.a survey of cultivated hybrid sugarcane (saccharum inter-specific hybrid) and noble sugarcane (saccharum officinarum) in southern china for the presence of sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv) and sugarcane streak mosaic virus (scsmv) was conducted by rt-pcr from the years 2003 to 2006. scmv and srmv, but not scsmv, were found. a high incidence of scmv and srmv coinfection was revealed in both hybrid and noble sugarcanes. all coinfected plants showed mosaic symptom, whereas ...200818438601
coat protein of potyviruses. i. comparison of the four australian strains of sugarcane mosaic virus.as an aid to a more rational classification of the potyvirus group, we have examined the molecular weight, amino acid composition, and tryptic peptide map of the coat protein of four australian sugarcane mosaic virus strains: johnson grass (jg), sugarcane (sc), queensland blue couch grass (bc), and sabi grass (sabi). the proteins migrated as a single band in sds-polyacrylamide gel electrophoresis with molecular weights of 33,700 (sc), 34,200 (jg), 39,100 (bc), and 40,300 (sabi). the bc and sabi ...198118635056
towards a system for the identification and classification of potyviruses. ii. virus particle length, symptomatology, and cytopathology of six distinct viruses.the mean particle lengths of lettuce mosaic virus (lmv), bean common mosaic virus (bcmv), passionfruit woodiness virus (pwv), potato virus y (pvy), sugarcane mosaic virus (scmv), and one strain of bean yellow mosaic virus (bymv) were all shown to be close to 750 nm. however, the length of two other serologically closely related strains of bymv, variants of pea mosaic virus referred to as pmv and spmv, had significantly longer particles, about 792 and 842 nm. each of the distinct viruses had read ...198118635068
the complete sequence of a sugarcane mosaic virus isolate causing maize dwarf mosaic disease in china.the complete sequence of a potyvirus from maize in zhejiang province was determined. the rna was 9596 nucleotides long, excluding the 3'-poly (a) tail, and there was a single long open reading frame (orf) of 9192 nts encoding a 346.1 ku polyprotein. the polyprotein had substantial amino acid sequence homology with those encoded by the rnas of a chinese isolate of sorghum mosaic virus (srmv-c) and a bulgarian isolate of maize dwarf mosaic virus, but it was most closely related to sugarcane mosaic ...200218759055
the pic19 nbs-lrr gene family members are closely linked to scmv1, but not involved in maize resistance to sugarcane mosaic virus.sugarcane mosaic virus (scmv) is the causal pathogen for a severe mosaic virus disease of maize worldwide. in our previous research, the maize resistance gene analog (rga) pic19 and its three cognate bac contigs were mapped to the same region as the scmv resistance gene scmv1. here we report the isolation and characterization of the pic19r gene family members from the inbred line fap1360a, which shows complete resistance to scmv. two primer pairs were designed based on the conserved regions amon ...200818772945
quantitative trait loci mapping of resistance to sugarcane mosaic virus in maize.abstract sugarcane mosaic virus (scmv) is an important virus disease of maize (zea mays) in europe. in this study, we mapped and characterized quantitative trait loci (qtl) affecting resistance to scmv in a maize population consisting of 219 f(3) or immortalized f(2) families from the cross of two european maize inbreds, d32 (resistant) x d145 (susceptible). resistance was evaluated in replicated field trials across two environments under artificial inoculation. the method of composite interval ...199918944678
sequence and relationships of sugarcane mosaic and sorghum mosaic virus strains and development of rt-pcr-based rflps for strain discrimination.abstract sugarcane mosaic is the most widespread virus disease affecting sugarcane production. we have established a collection of seven prominent sugarcane mosaic potyvirus (scmv) strains currently causing disease in sugarcane throughout the world and originally found in sugarcane in the united states. this collection includes scmv strains a, b, d, and e, and the sorghum mosaic virus (srmv) strains sch, sci, and scm. these viruses were propagated on sorghum bicolor cv. rio and purified. cloned ...199718945064
genetic diversity among viruses associated with sugarcane mosaic disease in tucumán, argentina.sugarcane leaves with mosaic symptoms were collected in 2006--07 in tucumán (argentina) and analyzed by reverse-transcriptase polymerase chain reaction (rt-pcr) restriction fragment length polymorphism (rflp) and sequencing of a fragment of the sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) coat protein (cp) genes. scmv was detected in 96.6% of samples, with 41% showing the rflp profile consistent with strain e. the remaining samples produced eight different profiles that did not ...200919055433
impact of 9 years of bt-maize cultivation on the distribution of maize viruses.this study assesses the effect of bt-maize on the distribution of maize viruses. random surveys were conducted in spain between 2001 and 2006 to evaluate the occurrence of maize viruses in bt-maize cultivation areas and in areas where this crop had not been introduced. maize dwarf mosaic virus (mdmv) was the predominant virus in bt-areas, and maize rough dwarf virus (mrdv) was the most predominant one in non-bt-areas, with mrdv an emergent virus in both types of areas. a decline in the occurrenc ...200919067216
rapid identification of viruses causing sugarcane mosaic by direct sequencing of rt-pcr products from crude extracts: a method for large scale virus surveys.sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) diversity studies are important to characterize virus populations in sugarcane producing areas, enabling (i) identification of shifts in predominant strains, (ii) detecting associations of strains with specific varieties, and (iii) possibly exposing the appearance of new strains which may affect the performance of varieties in a region. recent studies have shown significant sequence variability within scmv populations around the world ...200919152809
validation of candidate genes putatively associated with resistance to scmv and mdmv in maize (zea mays l.) by expression profiling.the potyviruses sugarcane mosaic virus (scmv) and maize dwarf mosaic virus (mdmv) are major pathogens of maize worldwide. two loci, scmv1 and scmv2, have ealier been shown to confer complete resistance to scmv. custom-made microarrays containing previously identified scmv resistance candidate genes and resistance gene analogs were utilised to investigate and validate gene expression and expression patterns of isogenic lines under pathogen infection in order to obtain information about the molecu ...200919187556
properties of virion transactivator proteins encoded by primate cytomegaloviruses.human cytomegalovirus (hcmv) is a betaherpesvirus that causes severe disease in situations where the immune system is immature or compromised. hcmv immediate early (ie) gene expression is stimulated by the virion phosphoprotein pp71, encoded by open reading frame (orf) ul82, and this transactivation activity is important for the efficient initiation of viral replication. it is currently recognized that pp71 acts to overcome cellular intrinsic defences that otherwise block viral ie gene expressio ...200919473490
simultaneous detection and identification of four sugarcane viruses by one-step rt-pcr.sugarcane mosaic disease (smd) caused by the sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv) and sugarcane streak mosaic virus (scsmv) and sugarcane yellow leaf disease (syld) caused by the sugarcane yellow leaf virus (scylv) are the two most prevalent and economically important viral diseases of sugarcane. in this study, a one-step quadruplex reverse transcription (rt)-pcr method that employed virus-specific primers was developed for the simultaneous detection and differentiation of ...200919646484
identification of new variants of scmv causing sugarcane mosaic in india and assessing their genetic diversity in relation to scmv type strains.sugarcane mosaic virus (scmv) is one of the two causative viruses of mosaic in sugarcane, a sugar crop widely grown under tropical and subtropical conditions worldwide. although molecular characterization of scmv strains was reported from many countries, strains occurring in india, a major sugarcane producer have not been reported so far. twenty-six sugarcane samples represented by seven major sugarcane growing states in india and usa were subjected to reverse-transcription polymerase chain reac ...200919757007
genetic and physical fine mapping of scmv2, a potyvirus resistance gene in maize.sugarcane mosaic virus (scmv) is an important virus pathogen both in european and chinese maize production, causing serious losses in grain and forage yield in susceptible cultivars. two major resistance loci confer resistance to scmv, one located on chromosome 3 (scmv2) and one on chromosome 6 (scmv1). we developed a large isogenic mapping population segregating in the scmv2, but not the scmv1 region, to minimize genetic variation potentially affecting expression of scmv resistance. we fine map ...201020155410
complete nucleotide sequence and taxonomy of sugarcane streak mosaic virus, member of a novel genus in the family potyviridae.the complete genomic sequence of a pakistani isolate of sugarcane streak mosaic virus (scsmv-pak) is determined to be 9782 nucleotides in length, excluding the 3' poly(a) tail, and it comprises a large open reading frame encoding a polyprotein of 3130 amino acid residues. the deduced polyprotein is likely to be cleaved at nine putative protease sites by three viral proteases to ten mature proteins. conserved motifs of orthologous proteins of other potyviruses are identified in corresponding posi ...201020162446
genomic evidence of intraspecific recombination in sugarcane mosaic virus.the sugarcane mosaic virus (scmv) of the genus potyvirus, which primarily affects maize, sugarcane, sorghum, abaca, and grasses, occurs worldwide and causes significant economic loss. using the full genome sequences of scmv and several recombination detection methods, in this study we report that recombination is the major driving force in the evolution and emergence of several new variants of scmv. we reported eight highly significant (p < 0.001) recombination break points, majority of which ar ...201021193953
short distance movement of genomic negative strands in a host and nonhost for sugarcane mosaic virus (scmv).in order to obtain an initial and preliminary understanding of host and nonhost resistance in the initial step of potyvirus replication, both positive and negative sugarcane mosaic virus (scmv) strands where traced in inoculated and systemic leaves in host and nonhost resistant maize and sugarcane for one mexican potyviral isolate (scmv-ver1). intermediary replication forms, such as the negative viral strand, seem to only move a short distance as surveyed by rt-pcr analysis and elisa in differen ...201121232133
duplex-immunocapture-rt-pcr for detection and discrimination of two distinct potyviruses naturally infecting sugarcane (saccharum spp. hybrid).a sensitive duplex-immunocapture-rt-pcr (d-ic-rt-pcr) technique was developed for detection and discrimination of taxonomically distinct sugarcane streak mosaic virus (scsmv) and sugarcane mosaic virus (scmv) that naturally infect sugarcane. d-ic-rt-pcr was performed using polyclonal antisera for capture of virions. oligo 5'-d(t)18(agc)-3' as a common reverse primer for both viruses and virus specific forward primers, 5'-aagtggttaaacgcctgtgg-3' and 5'-atgtc(ga)aagaa(ga)atgcgcttgc-3' were used fo ...201121365999
responses of maize (zea mays l.) near isogenic lines carrying wsm1, wsm2, and wsm3 to three viruses in the potyviridae.genes on chromosomes six (wsm1), three (wsm2) and ten (wsm3) in the maize (zea mays l.) inbred line pa405 control resistance to wheat streak mosaic virus (wsmv), and the same or closely linked genes control resistance to maize dwarf mosaic virus (mdmv) and sugarcane mosaic virus (scmv). near isogenic lines (nil) carrying one or two of the genes were developed by introgressing regions of the respective chromosomes into the susceptible line oh28 and tested for their responses to wsmv, mdmv, and sc ...201121667271
complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of sugarcane mosaic virus.sugarcane mosaic virus (scmv) is the most prevalent virus causing maize dwarf mosaic disease in northern china. a scmv isolate, bd8, was obtained from the maize showing dwarf and mosaic symptoms in baoding, china. the complete genomic sequence of bd8 is 9,576 nucleotides (nt) excluding the poly(a) tail. it contains one single open reading frame of 9,192-ánt and encodes a large polyprotein of 3,063-áamino acids (aa), flanked by a 5'-untranslated region (utr) of 148-ánt and a 3'-utr of 236-ánt. th ...201121833715
application of rna interference methodology to investigate and develop scmv resistance in maize.specific fragments of the sugarcane mosaic virus (scmv) coat protein gene (cp) were amplified by reverse transcriptionpolymerase chain reaction and used to construct a marker free small interfering rna complex expression vector against scmv. in planta transformation was performed on maize (zea mays) inbred line 8112 mediated by agrobacterium tumefaciens. pcr and southern blot analyses demonstrated successful integration of the cp segment into the 8112 genome. the in planta transformation frequen ...201425189224
maize-pathogen interactions: an ongoing combat from a proteomics perspective.maize (zea mays l.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. a considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify defense-associated proteins. in this review, we summarize interactions of maize with its agriculturally important pathogens that were assessed at the proteome level. employing diff ...201526633370
selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time pcr.both genome-wide transcriptomic surveys of the mrna expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus-plant interaction involve the precise estimation of the transcript abundance. quantitative real-time pcr (qpcr) is the most widely adopted technique for mrna quantification. in order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. nevertheless, the stabilit ...201323954326
maize elongin c interacts with the viral genome-linked protein, vpg, of sugarcane mosaic virus and facilitates virus infection.the viral genome-linked protein, vpg, of potyviruses is involved in viral genome replication and translation. to determine host proteins that interact with sugarcane mosaic virus (scmv) vpg, a yeast two-hybrid screen was used and a maize (zea mays) elongin c (zmelc) protein was identified. zmelc transcript was observed in all maize organs, but most highly in leaves and pistil extracts, and zmelc was present in the cytoplasm and nucleus of maize cells in the presence or absence of scmv. zmelc exp ...201424954157
bacterially expressed dsrna protects maize against scmv infection.rna interference (rnai) is a sequence-specific, posttranscriptional gene silencing (ptgs) process in plants that is mediated by dsrna homologous to the silenced gene(s). in this study, we report an efficient method to produce dsrna using a bacterial expression system. two fragments of the sugarcane mosaic virus (scmv) cp (coat protein) gene were amplified by rt-pcr, and cloned into the inverted-repeat cloning vector puccrnai. the two recombinant plasmids were transformed individually into e. col ...201020734050
iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences.iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ...201727632283
further characterization of maize chlorotic mottle virus and its synergistic interaction with sugarcane mosaic virus in maize.maize chlorotic mottle virus (mcmv) was first reported in maize in china in 2009. in this study we further analyzed the epidemiology of mcmv and corn lethal necrosis disease (clnd) in china. we determined that clnd observed in china was caused by co-infection of mcmv and sugarcane mosaic virus (scmv). phylogenetic analysis using four full-length mcmv cdna sequences obtained in this study and the available mcmv sequences retrieved from genbank indicated that chinese mcmv isolates were derived fro ...201728059116
synergistic infection of two viruses mcmv and scmv increases the accumulations of both mcmv and mcmv-derived sirnas in maize.the co-infection of maize chlorotic mottle virus (mcmv) and sugarcane mosaic virus (scmv) can cause maize lethal necrosis. however, the mechanism underlying the synergistic interaction between these two viruses remains elusive. in this study, we found that the co-infection of mcmv and scmv increased the accumulation of mcmv. moreover, the profiles of virus-derived sirnas (vsirnas) from mcmv and scmv in single- and co-infected maize plants were obtained by high-throughput sequencing. our data sho ...201626864602
genome-wide association and genomic prediction of resistance to maize lethal necrosis disease in tropical maize germplasm.genome-wide association analysis in tropical and subtropical maize germplasm revealed that mlnd resistance is influenced by multiple genomic regions with small to medium effects. the maize lethal necrosis disease (mlnd) caused by synergistic interaction of maize chlorotic mottle virus and sugarcane mosaic virus, and has emerged as a serious threat to maize production in eastern africa since 2011. our objective was to gain insights into the genetic architecture underlying the resistance to mlnd b ...201526152570
maize lethal necrosis (mln), an emerging threat to maize-based food security in sub-saharan africa.in sub-saharan africa, maize is a staple food and key determinant of food security for smallholder farming communities. pest and disease outbreaks are key constraints to maize productivity. in september 2011, a serious disease outbreak, later diagnosed as maize lethal necrosis (mln), was reported on maize in kenya. the disease has since been confirmed in rwanda and the democratic republic of congo, and similar symptoms have been reported in tanzania, uganda, south sudan, and ethiopia. in 2012, y ...201525822185
recombination analysis of maize dwarf mosaic virus (mdmv) in the sugarcane mosaic virus (scmv) subgroup of potyviruses.recombination among rna viruses is a natural phenomenon that appears to have played a significant role in the species development and the evolution of many strains. it also has particular significance for the risk assessment of plants which have been genetically modified for disease resistance by incorporating viral sequences into their genomes. however, the exact recombination events taking place in viral genomes are not investigated in detail for many virus groups. in this analysis, different ...201525392089
genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line.novel and previously known resistance loci for six phylogenetically diverse viruses were tightly clustered on chromosomes 2, 3, 6 and 10 in the multiply virus-resistant maize inbred line, oh1vi. virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. genetic resistance to different viruses has been characterized in maize populations in diverse environments using different screening techniques, and resistance loci hav ...201424500307
application of sequence-independent amplification (sia) for the identification of rna viruses in bioenergy crops.miscanthus x giganteus, energycane, and panicum virgatum (switchgrass) are three potential biomass crops being evaluated for commercial cellulosic ethanol production. viral diseases are potentially significant threats to these crops. therefore, identification of viruses infecting these bioenergy crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. the application is described of sequence-independent amplification, for the identif ...201020638415
a one-step real-time rt-pcr assay for the detection and quantitation of sugarcane streak mosaic virus.sugarcane mosaic disease is caused by the sugarcane streak mosaic virus (scsmv; genus poacevirus, family potyviridae) which is common in some asian countries. here, we established a protocol of a one-step real-time quantitative reverse transcription pcr (real-time qrt-pcr) using the taqman probe for the detection of scsmv in sugarcane. primers and probes were designed within the conserved region of the scsmv coat protein (cp) gene sequences. standard single-stranded rna (ssrna) generated by pcr- ...201526185758
transgenic sugarcane resistant to sorghum mosaic virus based on coat protein gene silencing by rna interference.as one of the critical diseases of sugarcane, sugarcane mosaic disease can lead to serious decline in stalk yield and sucrose content. it is mainly caused by potyvirus sugarcane mosaic virus (scmv) and/or sorghum mosaic virus (srmv), with additional differences in viral strains. rna interference (rnai) is a novel strategy for producing viral resistant plants. in this study, based on multiple sequence alignment conducted on genomic sequences of different strains and isolates of srmv, the conserve ...201525685813
development of a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for the detection of sugarcane mosaic virus and sorghum mosaic virus in sugarcane.a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detecting sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) in sugarcane. six sets of four primers corresponding to the conserved coat protein gene were designed and tested for each virus. three primer sets designed for detecting scmv and four for detecting srmv were successful in the rt-lamp assay. the effective primer sets were not only specific for their target virus, but also able to ...201525445794
isolation, expression and functional analysis of a putative rna-dependent rna polymerase gene from maize (zea mays l.).rna-dependent rna polymerases (rdrps) in plants have been reported to be involved in post-transcriptional gene silencing (ptgs) and antiviral defense. in this report, an rdrp gene from maize (zmrdrp1) was obtained by rapid amplification of cdna ends (race) and rt-pcr. the mrna of zmrdrp1 was composed of 3785 nucleotides, including a 167 nt 5' untranslated region (utr), a 291 nt 3'utr and a 3327 nt open reading frame (orf), which encodes a putative protein of 1108 amino acids with an estimated mo ...201019685166
field performance of transgenic sugarcane lines resistant to sugarcane mosaic virus.sugarcane mosaic disease is mainly caused by the sugarcane mosaic virus (scmv), which can significantly reduce stalk yield and sucrose content of sugarcane in the field. coat protein mediated protection (cpmp) is an effective strategy to improve virus resistance. a 2-year field study was conducted to compare five independent transgenic sugarcane lines carrying the scmv-cp gene (i.e., b2, b36, b38, b48, and b51) with the wild-type parental clone badila (wt). agronomic performance, resistance to s ...201728228765
an atypical thioredoxin imparts early resistance to sugarcane mosaic virus in maize.sugarcane mosaic virus (scmv) causes substantial losses of grain yield and forage biomass in susceptible maize worldwide. a major quantitative trait locus, scmv1, has been identified to impart strong resistance to scmv at the early infection stage. here, we demonstrate that zmtrxh, encoding an atypical h-type thioredoxin, is the causal gene at scmv1, and that its transcript abundance correlated strongly with maize resistance to scmv. zmtrxh alleles, whether they are resistant or susceptible, sha ...201728216424
molecular variability and distribution of sugarcane mosaic virus in shanxi, china.sugarcane mosaic virus (scmv) is responsible for large-scale economic losses in the global production of sugarcane, maize, sorghum, and some other graminaceous species. to understand the evolutionary mechanism of scmv populations, this virus was studied in shanxi, china. a total of 86 maize leaf samples (41 samples in 2012 and 45 samples in 2013) were collected from 4 regions of shanxi.201626987060
the complete genome sequences of two naturally occurring recombinant isolates of sugarcane mosaic virus from iran.sugarcane mosaic virus (scmv) is the most prevalent virus causing sugarcane mosaic and maize dwarf mosaic diseases. here, we presented the first two complete genomic sequences of iranian scmv isolates, nra and zra from sugarcane and maize. the complete genome sequences of nra and zra were, respectively, 9571 and 9572 nucleotides (nt) in length, excluding the 3'-terminal poly(a) tail. both isolates contained a 5'-untranslated region (utr) of 149 nt, an open reading frame of 9192 nt encoding a pol ...201626905544
characterization of sugarcane mosaic virus scmv1 and scmv2 resistance regions by regional association analysis in maize.sugarcane mosaic virus (scmv) causes one of the most severe virus diseases in maize worldwide, resulting in reduced grain and forage yield in susceptible cultivars. in this study, two association panels consisting of 94 inbred lines each, from china and the u.s., were characterized for resistance to two isolates: scmv-seehausen and scmv-bj. the population structure of both association panels was analyzed using 3072 single nucleotide polymorphism (snp) markers. the chinese and the u.s. panel were ...201526488483
nematode interactions with weeds and sugarcane mosaic virus in louisiana sugarcane.weeds did not appear to serve as reservoirs for phytophagous louisiana sugarcane nematode populations except for criconemella spp., meloidogyne spp., tylenchorhynchus annulatus, and total phytophagous nematode densities were lower on weed-stressed cane and were accompanied by reduced accumulations of free cysteine, proline, and 13 other free amino acids in sugarcane. a significant weed-virus interaction for sugarcane free cysteine accumulation was detected; t. annulatus populations were highly c ...199019287686
characterization of a novel potyvirus isolated from maize in israel.a potyvirus (proposed name of zea mosaic virus [zemv]) isolated from maize in israel was analyzed by serology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of capsid proteins, symptomatology, and sequencing. parts of the nuclear inclusion b, coat protein, and 3' regions were sequenced; the amino acid sequence of zemv capsid was determined by time-of-flight mass spectrometry (tofms). the results of these analyses were compared with those of similar analyses of the followin ...200018944557
characterization of small interfering rnas derived from sugarcane mosaic virus in infected maize plants by deep sequencing.rna silencing is a conserved surveillance mechanism against viruses in plants. it is mediated by dicer-like (dcl) proteins producing small interfering rnas (sirnas), which guide specific argonaute (ago)-containing complexes to inactivate viral genomes and may promote the silencing of host mrnas. in this study, we obtained the profile of virus-derived sirnas (vsirnas) from sugarcane mosaic virus (scmv) in infected maize (zea mays l.) plants by deep sequencing. our data showed that vsirnas which d ...201424819114
combined linkage and association mapping reveals candidates for scmv1, a major locus involved in resistance to sugarcane mosaic virus (scmv) in maize.sugarcane mosaic virus (scmv) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. maize resistance to scmv is associated with two dominant genes, scmv1 and scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. we combined both linkage and association mapping to identify positional candidate genes for scmv1.201324134222
proteomic and phytohormone analysis of the response of maize (zea mays l.) seedlings to sugarcane mosaic virus.sugarcane mosaic virus (scmv) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. control strategies have been developed, but only marginal successes have been achieved. for the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required.201323894637
comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus.sugarcane mosaic virus (scmv) is an important viral pathogen and has caused serious losses in grain and forage yield. to identify candidate scmv resistance proteins and to explore the molecular mechanisms involved in the plant-scmv interaction, we conducted proteomic analyses of leaf samples from resistant and susceptible ecotypes of maize infected with scmv. proteins were analyzed by quantitative two-dimensional differential gel electrophoresis (2d-dige), and 93 protein spots showed statistical ...201323770298
genetic diversity and population structure of sugarcane mosaic virus.sugarcane mosaic virus (scmv) is one of the causal pathogens of mosaic diseases on sugarcane, maize, sorghum and some other graminaceous species with worldwide distribution. the global genetic diversity and molecular evolution of scmv capsid protein (cp) gene were investigated with the nucleotide sequences available in the genbank database. phylogenetic analyses revealed that scmv isolates clustered in relation to their original hosts, and geographically distinct isolates from maize or sugarcane ...201323116592
identification of differentially regulated maize proteins conditioning sugarcane mosaic virus systemic infection.sugarcane mosaic virus (scmv) is the most important cause of maize dwarf mosaic disease. to identify maize genes responsive to scmv infection and that may be involved in pathogenesis, a comparative proteomic analysis was performed using the first and second systemically infected leaves (termed 1 sl and 2 sl, respectively). seventy-one differentially expressed proteins were identified in 1 sl and 2 sl upon scmv infection. among them, eight proteins showed the same changing patterns in both 1 sl a ...201728627019
modeling virus coinfection to inform management of maize lethal necrosis in kenya.maize lethal necrosis (mln) has emerged as a serious threat to food security in sub-saharan africa. mln is caused by coinfection with two viruses, maize chlorotic mottle virus and a potyvirus, often sugarcane mosaic virus. to better understand the dynamics of mln and to provide insight into disease management, we modeled the spread of the viruses causing mln within and between growing seasons. the model allows for transmission via vectors, soil, and seed, as well as exogenous sources of infectio ...201728535127
dissecting the molecular mechanism of the subcellular localization and cell-to-cell movement of the sugarcane mosaic virus p3n-pipo.the coding sequence of p3n-pipo was cloned by fusion pcr from sugarcane mosaic virus (scmv), a main causal agent of sugarcane (saccharum spp. hybrid) mosaic disease. scmv p3n-pipo preferentially localized to the plasma membrane (pm) compared with the plasmodesmata (pd), as demonstrated by transient expression and plasmolysis assays in the leaf epidermal cells of nicotiana benthamiana. the subcellular localization of the p3n-pipo mutants p3n-pipot1 and p3n-pipot2 with 29 and 63 amino acids delete ...201728852157
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