| differential effect of 2-thiouracil on synthesis of two plant viruses in the same host. | in cowpea leaves singly or doubly infected with cowpea chlorotic mottle virus (ccmv) and/or southern bean mosaic virus (sbmv), treatment with 2-thiouracil increased the accumulation of ccmv particles and strongly decreased the accumulation of sbmv particles. thiouracil prevented the usual inhibition of synthesis of ccmv particles at about 6 days after inoculation, and 2-3 times as many ccmv particles accumulated as in water-treated plants. a single treatment of thiouracil 48 h before, at the tim ... | 1977 | 870449 |
| coat protein properties suggest that azuki bean mosaic virus, blackeye cowpea mosaic virus, peanut stripe virus, and three isolates from soybean are all strains of the same potyvirus. | the interrelationship of a number of potyviruses infecting legumes has been investigated by comparing molecular properties of their coat proteins. comparison of the coat proteins by the techniques of amino acid analysis and page was inadequate to distinguish strains from distinct potyviruses. however, high-performance liquid chromatographic peptide profiles of tryptic digests of coat proteins of these legume-infecting potyviruses enabled such assignments to be made. these data indicate that amin ... | 1992 | 1500273 |
| differentiation of potyviruses and their strains by hybridization with the 3' non-coding region of the viral genome. | nucleic acid hybridization with the 3' non-coding region of the potyvirus genome as the probe was shown to be a relatively simple means of distinguishing between distinct potyviruses and their strains. comparisons of the nucleotide sequences of potyvirus genomes (ignoring gaps) showed that the degree of identity between equivalent genes of strains was greater than 96%, while between distinct potyviruses the identity ranged from 42% to 65%, suggesting that any extended sequence could be considere ... | 1992 | 1551936 |
| complete nucleotide sequences of two soybean mosaic virus strains differentiated by response of soybean containing the rsv resistance gene. | the complete nucleotide sequence of the genomic rnas of strains g2 and g7 of soybean mosaic virus were determined. in both cases, the genome is 9588 nucleotides long, excluding the 3'-terminal poly(a) sequence. a large open reading frame (nucleotides 132 to 9329) encodes a polyprotein of 3066 amino acids with a predicted m(r) of either 349542 (strain g2) or 349741 (strain g7). based on comparison with the proposed locations of cleavage sites of other potyvirus polyproteins, nine mature proteins ... | 1992 | 1645142 |
| coat protein of potyviruses. 7. amino acid sequence of peanut stripe virus. | the amino acid sequence of the 287-residue coat protein of peanut stripe virus (pstv) was determined from the sequences of overlapping peptide fragments. results indicated that the amino terminus was blocked by an acetyl group, as has previously been found for the coat protein of johnsongrass mosaic potyvirus. comparison of the pstv sequence with coat proteins of 20 distinct potyviruses gave sequence identities of 47-57%, except for zucchini yellow mosaic virus (zymv), passionfruit woodiness vir ... | 1991 | 1863222 |
| the complete nucleotide sequence of pea seed-borne mosaic virus rna. | the complete nucleotide sequence of the rna genome of pea seed-borne mosaic virus (psbmv) was determined from cloned cdna and by direct sequencing of viral rna. the psbmv genomic sequence was determined to be 9924 nucleotides in length excluding the poly(a) tract. the rna contained an open reading frame (orf) of 9618 nucleotides with the potential to encode a polyprotein with a calculated mr of 364000 (364k). the orf was flanked by a 5' untranslated leader sequence of 143 nucleotides and a 3' un ... | 1991 | 1940858 |
| nucleotide sequences of the coat protein genes of two aphid-transmissible strains of soybean mosaic virus. | the nucleotide sequences of the coat protein genes and 3' non-coding regions of two aphid-transmissible isolates (g2 and g7) of soybean mosaic virus (smv) were determined. the coat protein of the g2 isolate differs from that of the aphid non-transmissible n isolate by a single amino acid at position 12 (aspartic acid in n, glycine in g2 and g7). the g7 isolate differs from g2 and n at three and four amino acid residues, respectively. the nucleotide sequence similarity of the three isolates in th ... | 1991 | 2016589 |
| [a study of the inheritance and linkage of resistance to four strains of soybean mosaic virus in soybeans]. | ten susceptible x resistant and resistant x resistant crosses were made among eight varieties, of which seven were previously identified as resistant to one to four smv strains, in order to investigate the inheritance of resistance to the four strains (sa, sc, sg, and sh) and the linkage relationship among the resistant genes. seeds from f2 individual plants were randomly divided and planted in two separated nurseries, in which the seedlings were inoculated with two different strains. the genoty ... | 1991 | 2029427 |
| molecular genetic analyses of the soybean mosaic virus nia proteinase. | recombinant dna molecules containing cdna to a soybean mosaic virus (smv) rna genome were constructed and partial nucleotide sequences determined for two cdna inserts, psmv-34 and psmv-35. comparison of the predicted amino acid sequence encoded by the psmv-34 cdna insert to other potyvirus protein sequences revealed extensive homology with the region of the genome encoding the nia proteinase, with conservation of the amino acids proposed to form the catalytic triad of the active site. cell-free ... | 1990 | 2212987 |
| genome structure of tobacco necrosis virus strain a. | an almost complete sequence of the rna genome of tobacco necrosis virus (tnv) strain a has been determined. the genome organization is very similar to that of carnation mottle virus (carmv) and turnip crinkle virus (tcv). the 5'-proximal open reading frame (orf) encodes a 23-kda protein and read-through of its amber codon into the second orf is presumably used for the translation of a 82-kda protein. the third large orf encodes the 30-kda coat protein. two small orfs are located upstream and one ... | 1990 | 2371773 |
| watermelon mosaic virus ii and zucchini yellow mosaic virus: cloning of 3'-terminal regions, nucleotide sequences, and phylogenetic comparisons. | the 3'-terminal genomic regions of an isolate of watermelon mosaic virus ii (wmvii) and a florida isolate of zucchini yellow mosaic virus (zymv-f) have been cloned. the nucleotide sequence of the wmvii cdna clone shows the presence of the large nuclear inclusion protein gene, the coat protein gene and 3' untranslated region. the nucleotide sequence of a zymv-f cdna clone shows the presence of the coat protein gene and 3' untranslated region. comparisons of the nucleotide and deduced amino acid s ... | 1990 | 2374006 |
| the nucleotide sequence of a soybean mosaic virus coat protein-coding region and its expression in escherichia coli, agrobacterium tumefaciens and tobacco callus. | a dna complementary to the 3'-terminal 1168 nucleotides of the genome of the n strain of soybean mosaic virus (smv) has been cloned and sequenced. cdna sequence and coat protein analyses indicate that the smv coat protein-coding region is at the 3' end of the genome, and that the coat protein is processed from a larger protein. the coat protein-coding sequence is predicted to be 795 nucleotides in length, encoding a protein of 265 amino acids with a calculated mr of 29,857. the 3' untranslated r ... | 1989 | 2661723 |
| coat protein of potyviruses. 6. amino acid sequences suggest watermelon mosaic virus 2 and soybean mosaic virus-n are strains of the same potyvirus. | the amino acid sequence of the coat protein of watermelon mosaic virus 2 (wmv 2) was determined by a combination of peptide and nucleic acid sequencing. the coat protein of wmv 2 contained 281 amino acid residues including a single cysteine at position 132 and a blocked amino terminus. comparison with the coat protein sequences of 20 strains of ten distinct potyviruses showed sequence homologies ranging from 43% to 69% except for the n strain of soybean mosaic virus (smv-n), where the sequence h ... | 1989 | 2719555 |
| southern bean mosaic virus rna remains associated with swollen virions during translation in wheat germ cell-free extracts. | l-[35s]methionine-labeled translation complexes were prepared by incubating either swollen intact southern bean mosaic virus (sbmv) particles or unencapsidated sbmv rna in a wheat germ extract. the complexes were analysed by sucrose gradient centrifugation and by electron microscopy and dot blot hybridization of fractions from these gradients. in these complexes, 80 s ribosomes appeared to be associated with intact or near intact particles, suggesting that sbmv particles disassemble only after t ... | 1989 | 2763469 |
| the use of 3' non-coding nucleotide sequences in the taxonomy of potyviruses: application to watermelon mosaic virus 2 and soybean mosaic virus-n. | the sequence of the 3' 1106 nucleotides of the watermelon mosaic virus 2 (wmv 2) genome has been determined. the sequence contains the complete coding region of the viral coat protein followed by a 3' untranslated sequence of 251 nucleotides. when these sequences were compared with the equivalent regions of the n strain of soybean mosaic virus (smv-n), the coat protein coding regions were 82% homologous, whereas the 3' untranslated sequences were 78% homologous. optimal alignment of the 3' untra ... | 1989 | 2794980 |
| sequence and organization of southern bean mosaic virus genomic rna. | the genomic rna sequence of the cowpea strain of southern bean mosaic virus (sbmv-c) has been determined. the genome is 4194 nucleotides in length and has four open reading frames. a 5' proximal open frame, from base 49 to base 603, corresponds to the length of the p4 proteins translated in cell-free extracts from full-length and smaller virion rna. the largest open frame extends from base 570 to base 3437 and encodes the two largest proteins translated in cell-free extracts from full-length vir ... | 1987 | 2823471 |
| sobemovirus genome appears to encode a serine protease related to cysteine proteases of picornaviruses. | a putative serine protease was identified among non-structural proteins of southern bean mosaic virus (sbmv) by sequence comparison with cellular and viral proteases. the predicted sbmv protease displayed a significant similarity to cysteine proteases of picornaviruses, providing a possible evolutionary link between the two enzyme classes. it is suggested that sbmv follows the general expression strategy characteristic of other positive-strand rna viruses containing 5'-terminal covalently linked ... | 1988 | 3165867 |
| [intracellular inclusions of soybean mosaic virus]. | | 1968 | 5753639 |
| possibilities for inter- and intracellular translocation of some icosahedral plant viruses. | southern bean mosaic virus (sbmv) and tomato ringspot virus (tomrv) were compared with regard to possible ways of inter- and intracellular translocation. the pore complexes in the nuclear membranes of nuclei in leaf palisade and mesophyll cells of several plant species commonly used in plant virus research were studied. the pore structure resembled that earlier described. the diameter of the pores was great enough to allow icosahedral plant viruses between 25 and 30 mmicro wide to move through. ... | 1969 | 5765766 |
| detection of genomic-length soybean mosaic virus rna on polyribosomes of infected soybean leaves. | soybean mosaic virus (smv)-related rnas were examined in both polyribosomal and nonpolyribosomal fractions of systemically infected soybean leaves. viral rnas were detected by northern blot hybridization analysis using two cloned smv-cdnas representing different regions of the viral genome as hybridization probes. genomic length smv-rna (mr of 3.3 x 10(6] was found in specific association with edta-sensitive polyribosomes of infected leaves, indicating that it functions as a messenger rna in the ... | 1984 | 6388148 |
| rna-protein interactions in some small plant viruses. | the structure of the three quasi-equivalent protein subunits a, b and c of the spherical, t = 3 southern bean mosaic virus (sbmv) have been carefully built in accordance with a refined electron density map of the complete virus. the lower electron density in the rna portion of the map could not be explicitly interpreted in terms of a preferred rna structure on which some icosahedral symmetry might have been imposed. however, the extremely basic nature of the interior surface of the coat protein ... | 1983 | 6401119 |
| phosphorus-31 nuclear magnetic resonance study of internal motion in ribonucleic acid of southern bean mosaic virus. | phosphorus-31 nuclear magnetic resonance spectroscopy was used to study southern bean mosaic virus (sbmv) in its native and ethylenediaminetetraacetic acid treated forms at various ph values in the range 4.5-9.0. the degree of swelling in the latter form is a critical function of ph. spin-lattice relaxation time (t1), line-width, and nuclear overhauser effect (noe) measurements were made at 40.51, 80.98, and 190.3 mhz. the results indicate that the rna phosphate groups in native sbmv exhibit low ... | 1982 | 6816273 |
| detection of plant virus by using purified igg in elisa. | an optimized elisa system for the detection of soybean mosaic virus was developed. purified anti-soybean mosaic virus igg, a secondary ovalbumin coat, and a clearly defined optimal concentration of enzyme-conjugated anti-soybean mosaic virus igg are used. the test detects purified virus, or purified virus added to leaf and seed extracts, at a concentration of 2.5 ng/ml. various buffers significantly affect detection sensitivity. | 1981 | 7021576 |
| chemical and serological properties of a cyanogen bromide peptide of southern bean mosaic virus protein. | southern bean mosaic virus (sbmv) protein was cleaved with cyanogen bromide and a highly basic peptide, cb-1, was isolated by ion exclusion and ion-exchange chromatography. twelve peptides were separated from a tryptic digest of cb-1 by ion-exchange chromatography and the composition of these peptides was similar to that of peptides released from edta-swollen virus particles by limited tryptic digestion. the composition and n-termini of the tryptic peptides indicated cb-1 was from the n-terminus ... | 1980 | 7237270 |
| cdna cloning and sequence analysis of nib gene of soybean mosaic virus. | cdna of soybean mosaic virus (beijing isolate, smv-bj) has been synthesized, using viral genomic rna as a template and random hexanucleotides as primers. based on the sequences of smv-bj coat protein (cp) gene as well as smv- and wmv-ii-related regions, oligonucleotides were made as primers for polymerase chain reaction (pcr). nib gene of smv-bj was amplified by pcr, and cloned into pbluescript sk. the complete sequence was determined. the comparison of nib genes between smv-bj and wmv-ii (usa) ... | 1995 | 7755870 |
| nucleotide sequence of the bean strain of southern bean mosaic virus. | the genome of the bean strain of southern bean mosaic virus (sbmv-b) comprises 4109 nucleotides and thus is slightly shorter than those of the two other sequenced sobemoviruses (southern bean mosaic virus, cowpea strain (sbmv-c) and rice yellow mottle virus (rymv)). sbmv-b has an overall sequence similarity with sbmv-c of 55% and with rymv of 45%. three potential open reading frames (orfs) were recognized in sbmv-b which were in similar positions in the genomes of sbmv-c and rymv. however, there ... | 1995 | 7831784 |
| identification of a coat protein binding site on southern bean mosaic virus rna. | the cowpea strain of southern bean mosaic virus (sbmv-c), a t = 3 icosahedral rna virus, was dissociated to yield a ribonucleoprotein complex (rnpc) composed of the viral rna and coat protein subunits. to determine if the coat protein subunits were bound to a specific site on the viral rna, the rnpc was treated with ribonuclease, and the remaining coat protein--rna complexes were recovered by filter binding. a single species of rna was isolated by this procedure and further characterized by sequ ... | 1995 | 7886960 |
| primary structure of sesbania mosaic virus coat protein: its implications to the assembly and architecture of the virus. | sesbania mosaic virus (smv) is a plant virus that infects sesbania grandiflora plants in andhra pradesh, india. the amino acid sequence of the coat protein of smv was determined using purified peptides generated by cleavage with trypsin, chymotrypsin, v8 protease and clostripain. the 230 residues so far determined were compared to the corresponding residues of southern bean mosaic virus (sbmv), the type member of sobemoviruses. the overall identity between the sequences is 61.7%. the amino termi ... | 1994 | 8002015 |
| cowpea aphid borne mosaic virus-morocco and south african passiflora virus are strains of the same potyvirus. | high performance liquid chromatography (hplc) profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphid-borne mosaic virus (cabmv). some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus (b1cmv) while other reports have concluded that this relationship is distant. in this report a tryptic digest of the coat protein of cabmv-morocco was compared w ... | 1994 | 8002788 |
| cloning of coat protein gene of soybean mosaic virus and its expression in escherichia coli. | the 3'-terminal genomic region of the beijing isolate of soybean mosaic virus (smv-bj) has been cloned through technique of polymerase chain reaction (pcr). we have analyzed the nucleotide sequence of 3' region of smv-bj genome. comparisons of the nucleotide and deduced amino acid sequence of smv-bj coat protein gene with those of smv-n strain show 93.4% and 98.5% identity between them, respectively. alignments of the 3' non-coding sequence in pairs with that of smv-n strain show homology of 88. ... | 1993 | 8049345 |
| nucleotide sequence and genome organization of peanut stripe potyvirus. | a blotch isolate of peanut stripe virus (pstv) was cloned, sequenced and compared with other full-length potyvirus sequences. the viral genome was 10059 nucleotides (nt) in length excluding the poly(a) tail. two potential aug start codons were identified in the 5' non-translated region. analysis of in vitro translation products from transcripts containing the first 600 nt of the pstv genome indicated that the first aug (nt 134 to 136) was preferred over the second aug (nt 146 to 148) for initiat ... | 1994 | 8077957 |
| watermelon mosaic virus-morocco is a distinct potyvirus. | the relationship of the morocco isolate of watermelon mosaic virus (wmv) to wmv2, soybean mosaic virus (a virus closely related to wmv2) and the w strain of papaya ringspot virus (prsv-w), formerly wmv1, was examined by comparing tryptic peptide profiles using high performance liquid chromatography. the profiles indicated that the coat protein sequence of wmv-morocco differed substantially from those of the other potyviruses. this conclusion was supported by sequence data from five tryptic pepti ... | 1993 | 8347085 |
| failure of long-distance movement of southern bean mosaic virus in a resistant host is correlated with lack of normal virion formation. | sunn-hemp mosaic tobamovirus (shmv) facilitated the spread of the cowpea strain of southern bean sobemovirus (sbmv-c) only in inoculated leaves of common bean (phaseolus vulgaris l. cv. bountiful), a resistant host for sbmv-c. tissue prints of bean primary leaves doubly inoculated with shmv and sbmv-c, developed by western blotting, showed the presence of the sbmv-c capsid antigen in the mesophyll and epidermis, but no antigen was detected in the conducting bundles. typical sbmv-c virions were n ... | 1993 | 8376965 |
| structure of sesbania mosaic virus at 4.7 a resolution and partial sequence of the coat protein. | sesbania mosaic virus (smv) is a plant virus infecting sesbania grandiflora plants in andhra pradesh, india. amino acid sequence of the tryptic peptides of smv coat protein were determined using a gas phase sequenator. these sequences showed identical amino acids at 69% of the positions when aligned with the corresponding residues of southern bean mosaic virus (sbmv). crystals diffracting to better than 3 a resolution were obtained by precipitating the virus with ammonium sulphate. the crystals ... | 1993 | 8421301 |
| variation of proline rich cell wall proteins in soybean lines with anthocyanin mutations. | the i locus controls inhibition of anthocyanin accumulation in the epidermal cells of the soybean seed coat and affects abundance of prp1, a proline-rich cell wall protein in the seed coat. saline-soluble prp1 is abundant in the developing seed coats of cultivar richland (homozygous i, yellow), while it is significantly decreased in the pigmented isogenic mutant t157 (homozygous i, imperfect black). in this report, we examined soluble prp1 in several cultivars containing alleles of the i locus w ... | 1993 | 8425044 |
| nucleotide sequence, serology and symptomatology suggest that vanilla necrosis potyvirus is a strain of watermelon mosaic virus ii. | vanilla necrosis potyvirus (vnv) is the cause of significant losses to the south pacific islands vanilla crop. the gene for the coat protein of vnv has been cloned and sequenced. comparison of this gene with other potyviral coat protein sequences revealed 97% nucleotide sequence homology (98% amino acid homology) to a us isolate of watermelon mosaic virus ii (wmv-ii), 93% nucleotide sequence homology (96% amino acid homology) to an australian isolate of wmv-ii and 81% nucleotide sequence homolog ... | 1993 | 8470960 |
| structure of sesbania mosaic virus at 3 a resolution. | sobemoviruses are a group of rna plant viruses that have a narrow host range. they are characterized in vitro by their stability, high thermal inactivation point and longevity. the three-dimensional structure of only one virus belonging to this group, southern bean mosaic virus (sbmv), is known. structural studies on sesbania mosaic virus (smv), which is closely related to sbmv, will provide details of the molecular interactions that are likely to be important in the stability and assembly of so ... | 1995 | 8589997 |
| the complete nucleotide sequence and genome organization of bean common mosaic virus (nl3 strain). | the nucleotide sequences of 3 cdna clones corresponding to entire rna genome of bean common mosaic virus nl3 strain have been determined. the rna is 9612 nucleotides long, excluding a 3'-terminal poly(a) tail. a putative start codon located at nucleotide positions 170-172 initiates one large open reading frame that is terminated with a uaa codon at position 9368-9370. the predicted polyprotein has 3066 amino acids and an m(r) of 340.3 kda. the positions of putative protein cleavage sites have be ... | 1995 | 8607279 |
| mapping and expression of southern bean mosaic virus genomic and subgenomic rnas. | the coat protein of the cowpea strain of southern bean mosaic sobemovirus (sbmv-c) is translated from a subgenomic rna (sgrna) that is synthesized in the virus-infected cell. like the sbmv-c genomic rna, the sgrna has a viral protein (vpg) covalently bound to its 5' end. the mechanism(s) by which ribosomes initiate translation on the sbmv-c rnas is not known. to begin to characterize the translation of the sgrna it was first necessary to precisely map its 5' end. primer extension was used to ide ... | 1997 | 9268164 |
| structure of sesbania mosaic virus at 3 a resolution. | sesbania mosaic virus (smv) is an isometric, ss-rna plant virus found infecting sesbania grandiflora plants in fields near tirupathi, south india. the virus particles, which sediment at 116 s at ph 5.5, swell upon treatment with edta at ph 7.5 resulting in the reduction of the sedimentation coefficient to 108 s. smv coat protein amino acid sequence was determined and found to have approximately 60% amino acid sequence identity with that of southern bean mosaic virus (sbmv). the amino terminal 60 ... | 1997 | 9468608 |
| the 105-kda polyprotein of southern bean mosaic virus is translated by scanning ribosomes. | the cowpea strain of southern bean mosaic virus (sbmv-c) is a positive-sense rna virus. three open reading frames (orf-1, orf2, and orf3) are expressed from the genomic rna. the orf1 and orf2 initiation codons are located at nucleotide (nt) positions 49 and 570, respectively. orf1 is expressed by a 5' end-dependent scanning mechanism, but it is not known how ribosomes gain access to the orf2 initiation codon. in experiments described here, it was demonstrated that the translation of orf2 was sen ... | 1998 | 9656991 |
| the genome-linked protein (vpg) of southern bean mosaic virus is encoded by the orf2. | the sequence of the 20 n-terminal amino acids of the viral protein (vpg) which is covalently attached to the genomic rna of the bean strain of southern bean mosaic virus (sbmv-b) has been determined. the obtained vpg sequence mapped to position 327 to 346 of the sbmv-b orf2 product, downstream of the putative protease domain and in front of the rna-dependent rna polymerase. thus indicating that the sobemovirus genomic arrangement is similar to that of subgroup ii luteoviruses. comparison with ot ... | 1998 | 9778784 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| nucleotide sequence of a resistance breaking mutant of southern bean mosaic virus. | sbmv-s is a resistance-breaking mutant of an arkansas isolate of the bean strain of southern bean mosaic virus (sbmv-bark) that is able to move systemically in phaseolus vulgaris cvs. pinto and great northern, whereas the wild-type sbmv-bark causes local necrotic lesions and is restricted to the inoculated leaves of these hosts. sequence analysis of the 4136 nucleotide genomes of sbmv-bark and sbmv-s revealed seven nucleotide differences, but only four deduced amino acid changes. a single amino ... | 1998 | 9856101 |
| identification of viral genes required for cell-to-cell movement of southern bean mosaic virus. | inoculation of vigna unguiculata (cowpea) with transcripts synthesized in vitro from a genome-length cdna clone of the cowpea strain of southern bean mosaic virus (sbmv-c) resulted in a systemic sbmv-c infection of this host. capped rna was about five times more infectious than uncapped rna as determined by a local lesion assay. the sbmv-c cdna clone was also used for mutagenesis of the four sbmv-c open reading frames (orfs). orf1, orf3, and coat protein (cp) mutants were not infectious in cowpe ... | 1998 | 9878617 |
| complementation of the host range restriction of southern cowpea mosaic virus in bean by southern bean mosaic virus. | vigna unguiculata (cowpea) and phaseolus vulgaris (common bean) are permissive hosts for southern cowpea mosaic virus (scpmv) and southern bean mosaic virus (sbmv), respectively. neither of these two sobemoviruses systemically infects the permissive host of the other. although bean cells are permissive for scpmv rna synthesis, they do not support the assembly of this virus. thus, the host range restriction of scpmv in bean may occur at the level of movement and may involve the inability of scpmv ... | 2000 | 10612668 |
| mitigating epidemics caused by non-persistently transmitted aphid-borne viruses: the role of the pliant environment. | using soybean mosaic virus as a model system, the rate, magnitude and timing of epidemics caused by non-persistently transmitted, aphid-borne viruses are examined under various field conditions. emphasis is placed on the behavioural responses of vectors to environmental cues, although all three biotic components (host plant, vector and virus) are considered. both single and double manipulations of the cropping system environment are explored using a computer model developed earlier by ruesink an ... | 2000 | 11137172 |
| complete nucleotide sequence of sesbania mosaic virus: a new virus species of the genus sobemovirus. | the complete nucleotide sequence of the sesbania mosaic virus (semv) genomic rna was determined by sequencing overlapping cdna clones. the semv genome is 4149 nucleotides in length and encodes four potential overlapping open reading frames (orfs). comparison of the nucleotide sequence and the deduced amino acid sequence of the four orfs of semv with that of other sobemoviruses revealed that semv was closest to southern bean mosaic virus arkansas isolate (sbmv-ark, 73% identity). the 5' non-codin ... | 2001 | 11315633 |
| rsv1-mediated resistance against soybean mosaic virus-n is hypersensitive response-independent at inoculation site, but has the potential to initiate a hypersensitive response-like mechanism. | rsv1, a single dominant gene in soybean pi 96983, confers resistance to most strains of soybean mosaic virus (smv), including strain g2. the phenotypic response includes the lack of symptoms and virus recovery from mechanically inoculated leaves. to study the resistance mechanism, smv-n (an isolate of strain g2) was introduced into pi 96983 by grafting. hypersensitive response (hr)-like lesions occurred on the stems, petioles, and leaf veins, and virus was recovered from these lesions. the respo ... | 2001 | 11332723 |
| inheritance and allelism tests of raiden soybean for resistance to soybean mosaic virus. | the gene symbol rsv2 was previously assigned to the gene in the soybean [glycine max (l.) merr.] line ox670 for resistance to soybean mosaic virus (smv). the rsv2 gene was reported to be derived from the raiden soybean (pi 360844) and to be independent of rsv1. accumulated data from our genetic experiments were in disagreement with this conclusion. in this study, raiden and l88-8431, a williams bc5 isoline with smv resistance derived from raiden, were crossed with two smv-susceptible cultivars t ... | 2001 | 11336229 |
| a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family. | a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ... | 2001 | 11402861 |
| a mosaic disease of senna hirsuta induced by a potyvirus in nigeria. | a virus inducing mosaic and severe leaf malformation, isolated from senna hirsuta in nigeria, was studied. the virus had a rather narrow host range, infecting a few species in caesalpinaceae, chenopodiaceae and fabaceae families. the virus was widespread in southern nigeria with prevalence ranging from 74% to 86.4% in some locations. it was transmitted mechanically and in a non-persistent manner by myzuspersicae, aphis craccivora and a. spiraecola. there was no evidence of transmission by seeds. ... | 2001 | 11719985 |
| characterization of smv resistance genes in tousan 140 and hourei soybean. | 'tousan 140' and 'hourei', two soybean [glycine max (l) merr.] accessions from japan, each possess a single gene at different loci for resistance to japanese soybean mosaic virus (smv) strain smv c. however, more genetic information is needed to utilize these lines in a breeding program. the objectives of this study were to determine (i) the reaction of tousan 140 and hourei to smv-g1 through g7 strains, (ii) the inheritance of smv resistance in tousan 140 and hourei to strains smv-g1 and g7, an ... | 2002 | 11756258 |
| soybean mosaic virus (smv) and the smv resistance gene (rsv(1)): influence on phomopsis spp. seed infection in an aphid free environment. | infection of soybean [glycine max (l.) merr.] plants with soybean mosaic virus (smv) has been reported to enhance phomopsis spp. infection, which reduces seed quality. the timing and incidence of smv infection depends largely upon the level of primary inoculum and aphid-activity. two field experiments were conducted in aphid-free environments, to examine the influence of (i) smv-infection, and (ii) smv-resistance alleles of the rsv(1) gene, on the incidence of phomopsis spp. seed infection. in t ... | 2002 | 11756270 |
| genetic and sequence analysis of markers tightly linked to the soybean mosaic virus resistance gene, rsv3. | soybean mosaic virus (smv) is a major viral pathogen, affecting soybean [glycine max (l.) merr.] production worldwide. the rsv3 gene of soybean confers resistance to three of the most virulent strains (g5-g7) of smv. the objectives of this study were to map rsv3 and develop polymerase chain reaction (pcr) based markers for marker-assisted selection (mas) purposes. disease-response data were collected from two f(2) mapping populations, l29 (rsv3) x lee68 (rsv3) and tousan 140 (rsv3) x lee68 (rsv3 ... | 2002 | 11756284 |
| mapping tightly linked genes controlling potyvirus infection at the rsv1 and rpv1 region in soybean. | soybean mosaic virus (smv) and peanut mottle virus (pmv) are two potyviruses that cause yield losses and reduce seed quality in infested soybean (glycine max (l.) merr.) fields throughout the world. rsv1 and rpv1 are genes that provide soybean with resistance to smv and pmv, respectively. isolating and characterizing rsv1 and rpv1 are instrumental in providing insight into the molecular mechanism of potyvirus recognition in soybean. a population of 1056 f2 individuals from a cross between smv- a ... | 2002 | 12033629 |
| [distribution of soybean mosaic virus antigen in the leaves of soybean plants with different reaction to virus infection]. | the distribution and accumulation of soybean mosaic virus antigen in the leaves of sensitive plants with well expressed mosaic symptoms and symptomless tolerant soybean plants glycine max (l.) merr. were similar. low concentration of virus antigen was found in extremely resistant soybean plants in mechanically inoculated leaves and those of the 2-4 circles two-three weeks after inoculation but 5 weeks later the virus antigen was detected only in the top leaves. | 2002 | 12190033 |
| complementary action of two independent dominant genes in columbia soybean for resistance to soybean mosaic virus. | a stem-tip necrosis disease was observed in the soybean [glycine max (l.) merr.] cultivar columbia and its derivative ox686 when infected with a necrosis-causing strain of soybean mosaic virus (smv) in canada. a dominant gene named rsv3 was found in ox686 for the necrotic reaction. in the present research we have found that columbia is resistant to all known smv strains g1-g7, except g4. genetic studies were conducted to investigate the inheritance of resistance in columbia and interactions of r ... | 2002 | 12195033 |
| evidence that ribonuclease activity present in beetle regurgitant is found to stimulate virus resistance in plants. | phaseolus vulgaris l. cv. 'pinto' bean is a local lesion host for the plant pathogen southern bean mosaic virus (sbmv) and its vector is the mexican bean beetle, epilachna varivestis mulsant. the objective of this study was to determine if prior feeding by the beetle would affect 'pinto' bean's resistance to sbmv and determine if ribonuclease (rnase), a major constituent of beetle regurgitant, mediated the plant's response to the virus. 'pinto' bean plants fed upon by beetles had increased resis ... | 2002 | 12371820 |
| inheritance and allelism of resistance to soybean mosaic virus in zao18 soybean from china. | soybean mosaic disease caused by soybean mosaic virus (smv) occurs wherever soybean [glycine max (l.) merr.] is grown and is considered one of the most important soybean diseases in many areas of the world. use of soybean cultivars with resistance to smv is a very effective way of controlling the disease. china has rich soybean germplasm, but there is very limited information on genetics of smv resistance in chinese soybean germplasm and reaction of the resistance genes to smv strains g1-g7. the ... | 2002 | 12642647 |
| isolation and characterization of a full-length resistance gene homolog from soybean. | using mixed resistance gene analogs as probes, a putative resistance gene (kr1) was isolated from soybean and characterized further. the kr1 protein consists of a toll/interleukin receptor (tir) domain, a nucleotide binding site (nbs) domain, an imperfect leucine-rich repeat (lrr) domain and two c-terminal transmembrane segments. due to these features, kr1 represents a distinct member in the tir-nbs-lrr class of resistance genes. southern-blot analysis indicated that there were several kr1-relat ... | 2003 | 12647051 |
| genetic study of a lethal necrosis to soybean mosaic virus in pi 507389 soybean. | pi 507389 soybean [glycine max (l.) merr.], a large-seeded line from japan, exhibits a rapid, lethal, necrotic response to strains g1, g2, g5, and g6 of soybean mosaic virus (smv). unlike the hypersensitive necrotic reaction, this stem-tip necrosis can be a serious threat to soybean production. to investigate the genetic basis of lethal necrosis (ln), pi 507389 was crossed with the susceptible (s) cv. lee 68 and with resistant (r) lines pi 96983, cv. york, and cv. marshall, which carry single do ... | 2003 | 12816960 |
| molecular analysis of the 3' terminal region of the genome of beet mosaic virus and its relation with other potyviruses. | a1633 nucleotides long 3'-terminal sequence of genome of the beet mosaic virus (btmv) ren1 isolate from slovakia, spanning a part of the nib gene, complete capsid protein (cp) gene and a 3'non-coding region was determined. computer-assisted translation resulted in a polypeptide encompassing typical conserved potyviral amino acid (aa) motifs. homology blast search and phylogenetic analysis revealed that btmv was closest to bean common mosaic virus, soybean mosaic virus and zucchini yellow mosaic ... | 2003 | 14505096 |
| variability and transmission by aphis glycines of north american and asian soybean mosaic virus isolates. | the variability of north american and asian strains and isolates of soybean mosaic virus was investigated. first, polymerase chain reaction (pcr) products representing the coat protein (cp)-coding regions of 38 smvs were analyzed for restriction fragment length polymorphisms (rflp). second, the nucleotide and predicted amino acid sequence variability of the p1-coding region of 18 smvs and the helper component/protease (hc/pro) and cp-coding regions of 25 smvs were assessed. the cp nucleotide and ... | 2003 | 14551816 |
| evolution of soybean mosaic virus-g7 molecularly cloned genome in rsv1-genotype soybean results in emergence of a mutant capable of evading rsv1-mediated recognition. | plant resistance (r) genes direct recognition of pathogens harboring matching avirluent signals leading to activation of defense responses. it has long been hypothesized that under selection pressure the infidelity of rna virus replication together with large population size and short generation times results in emergence of mutants capable of evading r-mediated recognition. in this study, the rsv1/soybean mosaic virus (smv) pathosystem was used to investigate this hypothesis. in soybean line pi ... | 2003 | 14554079 |
| a virus related to soybean mosaic virus from pinellia ternata in china and its comparison with local soybean smv isolates. | a potyvirus isolated from pinellia ternata in china was characterised and shown to be related to soybean mosaic virus (smv). the virus was pathogenic on p. ternata and some soybean cultivars, whereas the local soybean smv isolate hh5 did not infect p. ternata. western blot experiments demonstrated a serological relationship between the virus from pinellia, smv and watermelon mosaic virus (wmv). the complete nucleotide sequences of the pinellia virus (isolate p-1, 9735 nt) and of the chinese soyb ... | 2004 | 14745600 |
| recombination within a nucleotide-binding-site/leucine-rich-repeat gene cluster produces new variants conditioning resistance to soybean mosaic virus in soybeans. | the soybean rsv1 gene for resistance to soybean mosaic virus (smv; potyvirus) has previously been described as a single-locus multi-allelic gene mapping to molecular linkage group (mlg) f. various rsv1 alleles condition different responses to the seven (g1-g7) described strains of smv, including extreme resistance, localized and systemic necrosis, and mosaic symptoms. we describe the cloning of a cluster of nbs-lrr resistance gene candidates from mlg f of the virus-resistant soybean line pi96983 ... | 2004 | 15020438 |
| genetics of resistance to two strains of soybean mosaic virus in differential soybean genotypes. | there are seven pathotypes of soybean mosaic virus (smv) representing seven strain groups (g1-g7) in the united states. soybean genotypes [glycine max (l.) merr.] may exhibit resistant (r), susceptible (s), or necrotic (n) reactions upon interacting with different smv strains. this research was conducted to investigate whether reactions to two smv strains are controlled by the same gene or by separate genes. two smv-resistant soybean lines, lr1 and lr2, were crossed with the susceptible cultivar ... | 2004 | 15247311 |
| the nucleotide sequence of watermelon mosaic virus (wmv, potyvirus) reveals interspecific recombination between two related potyviruses in the 5' part of the genome. | watermelon mosaic virus (wmv, potyvirus) is a potyvirus with a worldwide distribution, mostly in temperate and mediterranean regions. according to the partial sequences that were available, wmv appeared to share high sequence similarity with soybean mosaic virus (smv), and it was almost considered as a strain of smv in spite of its different and much broader host range. like smv, it was also related to legume-infecting potyviruses belonging to the " bean common mosaic virus (bcmv) subgroup". in ... | 2004 | 15290385 |
| characterization of an nbs-lrr resistance gene homologue from soybean. | conserved motifs such as the nucleotide-binding site (nbs) were found in many characterized plant disease resistance genes. based on the nbs domain, resistance gene analogs have been isolated in our previous study and were used as probes to screen a soybean (glycine max) cdna library. a full-length cdna, kr4, was isolated by screening the library and rapid amplification of cdna ends method. sequence analysis revealed that the cdna was 3818 bp in length and the open reading frame coded for a poly ... | 2004 | 15310071 |
| a protein interaction map of soybean mosaic virus strain g7h based on the yeast two-hybrid system. | a protein interaction map of soybean mosaic virus (smv) strain g7h was generated by the yeast two-hybrid system. clones encoding the genes p1, hc-pro, p3, 6k1, ci, 6k2, vpg, nia, nib, and cp were fused downstream of the gal4 binding domain (gal4-bd) and of the gal4 activation domain (gal4-ad). the gal4-bd and gal4-ad fusion derivatives of each gene were co-transformed into yeast and transformants in which interaction took place were identified on selective media. interacting fusion proteins were ... | 2004 | 15359133 |
| further molecular characterisation of potyviruses infecting aroid plants for medicinal use in china. | degenerate primers were used to detect and amplify 3'-terminal genome fragments of potyviruses from medicinal aroid plants growing at 16 sites in china. virus was detected in 7 samples of which six, all of pinellia ternata, contained a strain of soybean mosaic virus (smv) similar to that previously reported from this host in china. the complete sequence of one isolate and the p1 protein coding region of the other isolates were also sequenced. in all cases, the p1 proteins resembled isolates of d ... | 2005 | 15449140 |
| [mapping of five genes resistant to smv strains in soybean]. | soybean mosaic virus (smv) is one of the most prevalent pathogens that impact the soybean world widely. previous reports showed that most of the resistances were controlled by one pair of dominant genes. in this study, a soybean ril population njriky derived from kefeng 1 x nannong 1138-2 was used to study the inheritance of resistance to five smv strains (sa,sc-8, sc-9, n1, and n3) and mapping of resistant genes. kefeng 1 is resistant to all five smv strains while nannong 1138-2 is susceptible ... | 2004 | 15468924 |
| loss and gain of elicitor function of soybean mosaic virus g7 provoking rsv1-mediated lethal systemic hypersensitive response maps to p3. | rsv1, a single dominant resistance gene in soybean pi 96983 (rsv1), confers extreme resistance against all known american strains of soybean mosaic virus (smv), except g7 and g7d. smv-g7 provokes a lethal systemic hypersensitive response (lshr), whereas smv-g7d, an experimentally evolved variant of smv-g7, induces systemic mosaic. to identify the elicitor of rsv1-mediated lshr, chimeras were constructed by exchanging fragments between the molecularly cloned smv-g7 (psmv-g7) and smv-g7d (psmv-g7d ... | 2005 | 15613348 |
| cloning and characterization of an hdzip i gene gmhz1 from soybean. | by using cdna-aflp, we analyzed a recombinant inbred line population of soybean that was derived from a soybean mosaic virus (smv) resistant cultivar kefeng no.1 and a susceptible cultivar nannong 1138-2. one hundred and eight fragments showing polymorphism between smv resistant and susceptible pools were identified. one fragment w27 was 96 bp in length and showed homology to homeobox ggth with a coding region of 738 bp, encoding a protein of 245 amino acids. the genomic sequence analysis define ... | 2005 | 15754189 |
| emergence of rsv-resistance breaking soybean mosaic virus isolates from korean soybean cultivars. | twelve rsv resistance-breaking (rb) isolates of soybean mosaic virus (smv) were obtained from field-grown soybean plants showing mosaic symptoms and subsequently examined biologically and molecularly. all of these rb isolates were identified as smv based on serological and infectivity assays, and the amplification of p1 gene products by reverse transcription-polymerase chain reaction (rt-pcr). differential soybean cultivars, lines or accessions lee 68 (rsv), pi 96983, york, marshall, ogden, kwan ... | 2005 | 15869819 |
| [construction and primary analysis of subtractive library induced by soybean mosaic virus (smv)]. | smv is one of main diseases of soybean, which could affect yields and quality of soybean seriously. it was effective to soybean breeding by studying the expression of resistant gene to smv with molecular technology. in this study, a soybean resistance line, dongnong 8143, was used to construct a subtractive cdna library by ssh from soybean leaves inoculated by smv no.1 at primary stage. cdna dominantly or specifically expressed in infected leaves was purified using pcr purification kit and clone ... | 2005 | 16013498 |
| development of bean pod mottle virus-based vectors for stable protein expression and sequence-specific virus-induced gene silencing in soybean. | plant virus-based vectors provide valuable tools for expression of foreign proteins in plants and for gene function studies. none of the presently available virus vectors is suitable for use in soybean. in the present study, we produced bean pod mottle virus (bpmv)-based vectors that are appropriate for gene expression and virus-induced gene silencing (vigs) in soybean. the genes of interest were inserted into the rna2-encoded polyprotein open reading frame between the movement protein (mp) and ... | 2006 | 16226780 |
| strain-specific p3 of soybean mosaic virus elicits rsv1-mediated extreme resistance, but absence of p3 elicitor function alone is insufficient for virulence on rsv1-genotype soybean. | when challenged by mechanical inoculation, the rsv1 gene of soybean invokes extreme resistance (er) against soybean mosaic virus (smv) strain n, but not smv-g7 and its experimentally evolved variant, smv-g7d. smv-g7 provokes a lethal systemic hypersensitive response (lshr), whereas smv-g7d induces systemic mosaic. thus, for rsv1-genotype soybean, smv-g7 and smv-g7d are both virulent virus strains. the elicitor function of smv-g7 provoking rsv1-mediated lshr was recently mapped to p3, and the inf ... | 2006 | 16263149 |
| soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean. | soybean mosaic virus (smv) helper component protease (hc-pro), a suppressor of post-transcriptional gene silencing, was evaluated for its ability to enhance production of soybean hygromycin-resistant somatic embryos (hr-ses), and stabilize transgene expression. immature soybean cotyledonary explants were co-cultured with agrobacterium tumefaciens strain kyrt1 harboring either pcambia1302, carrying a hygromycin phosphotransferase gene (hpt) and a gene encoding green fluorescent protein; pcambia13 ... | 2005 | 16316753 |
| detection and characterisation of a second potyvirus from thunberg fritillary in china. | plants of thunberg fritillary (fritillaria thunbergii miq.) from zhejiang province, were found to be co-infected with two distinct potyviruses. one was an isolate of the recently reported thunberg fritillary mosaic virus (tfmv; wei et al., (2005) arch virol 150: 1271-1280), while the other was a distinct virus that did not react with tfmv antiserum nor with antisera to 17 other potyviruses, except for a weak reaction with antibodies produced to soybean mosaic virus (smv) pinellia strain. both vi ... | 2006 | 16328133 |
| importance of the c-terminal domain of soybean mosaic virus coat protein for subunit interactions. | the potyvirus coat protein (cp) is involved in aphid transmission, cell-to-cell movement and virus assembly, not only by binding to viral rna, but also by self-interaction or interactions with other factors. in this study, a number of cp mutants of soybean mosaic virus (smv) containing deletions and site-directed mutations were generated and cloned into yeast two-hybrid vectors. interaction was confirmed by the expression of reporter genes, including his3, ade2 and mel1, in yeast strain ah109. d ... | 2006 | 16361435 |
| pseudomonas syringae effector avrb confers soybean cultivar-specific avirulence on soybean mosaic virus adapted for transgene expression but effector avrpto does not. | soybean mosaic virus (smv) was adapted for transgene expression in soybean and used to examine the function of avirulence genes avrb and avrpto of pseudomonas syringae pvs. glycinea and tomato, respectively. a cloning site was introduced between the p1 and hc-pro genes in 35s-driven infectious cdnas of strains smv-n and smv-g7. insertion of the uida gene or the green fluorescent protein gene into either modified cdna and bombardment into primary leaves resulted in systemic expression that reflec ... | 2006 | 16570660 |
| replication of pea enation mosaic virus rna in isolated pea nuclei. | isolated nuclei from healthy pea plants were primed with pea enation mosaic virus (pemv), southern bean mosaic virus (sbmv), radish mosaic virus (rdmv), tobacco mosaic virus (tmv), pemv rna, sbmv rna, rdmv rna, or tmv rna. rna replication occurred only with pemv rna and not with intact pemv or any of the other viruses or rnas, as judged by ensuing actinomycin d-insensitive polymerase activity. molecular hybridization experiments showed that some of the product of the polymerase was pemv-specific ... | 1977 | 16592421 |
| application of comparative genomics in developing molecular markers tightly linked to the virus resistance gene rsv4 in soybean. | the rsv4 gene confers resistance to all the known strain groups of soybean mosaic virus in soybean (glycine max (l.) merr.). to construct a fine genetic map near rsv4 in soybean, we employed a comparative genomics approach that used genome sequence information of the model legume lotus japonicus. sequences of the soybean expressed sequence tags (ests) ai856415 and bf070293 mapping to one side of the rsv4 gene showed high similarity with gene sequences of the transformation-competent artificial c ... | 2006 | 16699558 |
| the genomic sequence of wisteria vein mosaic virus and its similarities with other potyviruses. | the complete nucleotide sequence of a beijing isolate of wisteria vein mosaic virus was determined to be 9695 nucleotides in length excluding the poly(a) tail. sequence analysis predicted a single large open reading frame of 9279 nucleotides potentially encodes a polyprotein of 3092 amino acids. phylogenetic analysis based on the genomic and deduced amino acid sequences support the current status of wisteria vein mosaic virus (wvmv) as a distinct virus of the genus potyvirus and a member of the ... | 2006 | 16732423 |
| the complete nucleotide sequence of a pakistani isolate of watermelon mosaic virus provides further insights into the taxonomic status in the bean common mosaic virus subgroup. | watermelon mosaic virus (wmv) is a potyvirus with a worldwide distribution, but is mostly found in temperate and mediterranean regions. the complete nucleotide (nt) sequence of a pakistani isolate of wmv (wmv-pk) was determined and compared with french isolate (wmv-fr) and other closely related potyviruses. wmv-pk showed overall identities of 94.4% (nt) and 96% (amino acid; aa) with the wmv-fr. however, variability was observed in the 5' utr and p1 region. although sequence identities over most ... | 2006 | 16732483 |
| [ultrastructure of parenchymal leaf cells of different soybean varieties systemically infected with soybean mosaic virus]. | a comparative study was made of the ultrastructure of parenchyma leaf cells of different soybean varieties systemically infected with soybean mosaic virus (smv). it has been shown that virus accumulation and formation of virus-specific cylindrical inclusions (cis) occur in the infected cells, in addition to intracellular changes showing stimulation of lytic processes, such as activation of smooth endoplasmic reticulum and golgi apparatus, formation of cytoplasmic vacuoles, cytosegresomes, myelin ... | 2006 | 16805310 |
| genetic analysis of resistance to soybean mosaic virus in j05 soybean. | soybean cultivar j05 was identified to be resistant to the most virulent strain of soybean mosaic virus (smv) in northeastern china. however, the reaction of j05 to smv strains in the united states of america is unknown, and genetic information is needed to utilize this germplasm in a breeding program. the objectives of this study were to determine the reaction of j05 to all us strains of smv (g1-g7), the inheritance of smv resistance in j05, and the allelic relationship of resistance genes in j ... | 2006 | 16990409 |
| mapping of smv resistance gene rsc-7 by ssr markers in soybean. | soybean mosaic virus (smv) is one of the most prevalent pathogens that limit soybean production. in this study, segregation ratios of resistant plants to susceptible plants in p1, p2, f1, f2 populations of kefeng no. 1 (p1) x nannong 1138-2 (p2) and derived ril populations, were used to study the inheritance of resistance to the smv strain sc-7. populations kefeng no. 1 and f1 were found to be completely resistant to this smv strain while nannong 1138-2 was susceptible to it. the f2 and ril popu ... | 2006 | 17028941 |
| inheritance analysis of herbicide-resistant transgenic soybean lines. | four transgenic soybean lines generated via agrobacterium-mediated transformation were used to analyze inheritance of the transgenes. seed chip gus assay and herbicide leaf painting and spraying assays were applied to test the gus reporter gene and the herbicide resistant bar selectable marker gene, respectively. three of the four transgenic soybean lines were stably inherited in a mendelian fashion with co-segregation of both transgenes in a 3:1 segregation ratio in the t(1) progeny, indicating ... | 2006 | 17185170 |
| a method for quantitative analysis of spatially variable physiological processes across leaf surfaces. | many physiological processes are spatially variable across leaf surfaces. while maps of photosynthesis, stomatal conductance, gene expression, water transport, and the production of reactive oxygen species (ros) for individual leaves are readily obtained, analytical methods for quantifying spatial heterogeneity and combining information gathered from the same leaf but with different instruments are not widely used. we present a novel application of tools from the field of geographical imaging to ... | 2007 | 17211583 |
| interactions of viruses in cowpea: effects on growth and yield parameters. | the study was carried out to investigate the effects of inoculating three cowpea cultivars: "olo ii", "oloyin" and it86d-719 with three unrelated viruses: cowpea aphid-borne mosaic virus (cabmv), genus potyvirus, cowpea mottle virus (cmev), genus carmovirus and southern bean mosaic virus (sbmv), genus sobemovirus singly and in mixture on growth and yield of cultivars at 10 and 30 days after planting (dap). generally, the growth and yield of the buffer inoculated control plants were significantly ... | 2007 | 17286870 |
| rapid diagnosis of soybean mosaic virus n soybean by tissue-print immunoassay and diba in comparison to other serological methods. | soybean mosaic virus (smv) is an important disease in soybean and is widely distributed in northern of iran. smv transmitted by soybean seed and detection of it is very important for disease management. in this study, several detection methods including das-elisa, indirect-elisa, tissue-print immunoassay (tpia) and dot immunobinding assay (diba) were optimized and compared with each other to identify the virus, using polyclonal antibody. for tpia, nitrocellulose membrane was used to imprint fres ... | 2006 | 17390880 |
| the '6k1' protein of a strain of soybean mosaic virus localizes to the cell periphery. | the '6k1' protein of the pinellia isolate of soybean mosaic virus was cloned into a prokaryotic expression vector and a polyclonal antiserum raised to the expressed fusion protein. in immunogold labeling of thin sections of infected leaves of pinellia ternata, specific labeling occurred at the cell periphery. this might suggest that the potyvirus '6k1' protein plays some role in viral cell-to-cell movement but the lack of transmembrane domains suggests that it does not conform to currently-recog ... | 2007 | 17458620 |
| cowpea viruses: effect of single and mixed infections on symptomatology and virus concentration. | natural multiple viral infections of cultivated cowpeas have been reported in nigeria. in this study, three nigerian commercial cowpea cultivars ("olo 11", "oloyin" and "white") and two lines from the iita (it86d- 719 and tvu 76) were mechanically inoculated with cowpea aphid-borne mosaic virus (cabmv), bean southern mosaic virus (sbmv) and cowpea mottle virus (cmev) singly, as well as in all possible combinations at 10, 20 and 30 days after planting (dap). samples of leaves or stems were collec ... | 2007 | 17900355 |
| molecular characterization and evolutionary analysis of soybean mosaic virus infecting pinellia ternata in china. | twenty-nine pinellia ternata specimens were collected from representative areas in china, including the major production provinces of zhejiang, henan, shanxi, hunan, shandong and hubei. seven isolates related to soybean mosaic virus (smv), which could be pathogenic on p. ternata and some soybean [glycine max (l.) merr.] cultivars, were detected using double antibody sandwich immunosorbent assay (das-elisa) and rt-pcr amplification performed with degenerate primer of potyviruses. it is revealed t ... | 2008 | 18058013 |
| association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection. | compatible virus infection induces and suppresses host gene expression at the global level. these gene-expression changes are the molecular basis of symptom development and general stress and defence-like responses of the host. to assess transcriptional changes in soybean plants infected with soybean mosaic virus (smv), the first soybean trifoliate leaf, immediately above the smv-inoculated unifoliate leaf, was sampled at 7, 14 and 21 days post-inoculation (p.i.) and subjected to microarray anal ... | 2008 | 18343851 |
| genetic confirmation of 2 independent genes for resistance to soybean mosaic virus in j05 soybean using ssr markers. | j05 soybean was previously identified to carry 2 independent genes, rsv1 and rsv3, for "soybean mosaic virus" (smv) resistance by inheritance and allelism studies. the objective of this research was to confirm the 2 genes in j05 using molecular markers so that a marker-assisted selection can be implemented. the segregation of f(2) plants from j05 x essex exhibited a good fit to a 3:1 ratio when inoculated with smv g1. three simple sequence repeat (ssr) markers near rsv1, satt114, satt510, and sa ... | 2008 | 18492653 |
| gain of virulence on rsv1-genotype soybean by an avirulent soybean mosaic virus requires concurrent mutations in both p3 and hc-pro. | in soybean, rsv1, a single dominant resistance gene, invokes extreme resistance (er) against most soybean mosaic virus (smv) strains, including smv-n, but not smv-g7, which provokes a virulent lethal systemic hypersensitive response (lshr). the elicitor functions of the two viruses provoking rsv1-mediated er and lshr have been mapped to the n-terminal 271 amino acids of p3 from smv-n and smv-g7, respectively, which differ by nine residues between the two strains. to identify amino acids of p3 fr ... | 2008 | 18533833 |