| procedures for the efficient purification of pea seed-borne mosaic virus and its genomic rna. | an efficient protocol for the purification of pea seed-borne mosaic potyvirus (psbmv) particles was developed. this led to the purification of 10 psbmv isolates by a single procedure. virus aggregation during purification did not occur and consequently, high virus yield was consistently obtained. the virus thus purified was suitable for preparing viral genomic rna, although conventional methods for rna extraction resulted in rna degradation. an alternative method was adopted which yielded reprod ... | 1992 | 1560106 |
| the 5' untranslated region from pea seedborne mosaic potyvirus rna as a translational enhancer in pea and tobacco protoplasts. | we have exploited the transient expression of foreign genes introduced into plant protoplasts to investigate the effect of the pea seedborne mosaic potyvirus (psbmv) 5' untranslated region (5'utr) on the level of gene expression in pea and tobacco protoplasts. the plant viral 5'utrs were found to increase translation significantly in comparison to a plasmid containing no 5'utr of viral origin. the enhancement effect of the 5'utrs of psbmv and tobacco etch potyvirus (tev) was found to be similar ... | 1992 | 1607015 |
| early embryo invasion as a determinant in pea of the seed transmission of pea seed-borne mosaic virus. | seed transmission of an isolate of pea seed-borne mosaic virus (psbmv) in several pea genotypes has been studied. cross-pollination experiments showed that pollen transmission of psbmv did not occur and accordingly, virus was not detected in pollen grains by elisa or electron microscopy. comparative studies between two pea cultivars, one with a high incidence of seed transmission and one with none, showed that psbmv infected the floral tissues (sepals, petals, anther and carpel) of both cultivar ... | 1992 | 1629692 |
| nucleotide sequence of pea seed-borne mosaic potyvirus coat protein gene. | cdna of pea seed-borne mosaic potyvirus (psbmv) rna was synthesized and cloned in e. coli. four overlapping clones that cover the complete psbmv genome, except the extreme 5' terminus, were identified by restriction enzyme mapping, hybridization analysis, and partial sequencing. overlapping cdna clones covering 1386 nucleotides of the 3' terminus were sequenced. the nucleotide sequence contains one open reading frame (orf), followed by an untranslated region of 163 nucleotides and a poly(a)-trac ... | 1991 | 1796554 |
| the complete nucleotide sequence of pea seed-borne mosaic virus rna. | the complete nucleotide sequence of the rna genome of pea seed-borne mosaic virus (psbmv) was determined from cloned cdna and by direct sequencing of viral rna. the psbmv genomic sequence was determined to be 9924 nucleotides in length excluding the poly(a) tract. the rna contained an open reading frame (orf) of 9618 nucleotides with the potential to encode a polyprotein with a calculated mr of 364000 (364k). the orf was flanked by a 5' untranslated leader sequence of 143 nucleotides and a 3' un ... | 1991 | 1940858 |
| nucleotide sequence of the coat protein gene of pea seed-borne mosaic potyvirus. | the nucleotide sequence of a 1355 bp cdna representing the 3'-terminal sequences of pea seed-borne mosaic virus (psbmv) was determined. this sequence contained a single long open reading frame (orf) of 1189 bp ending with a single taa termination codon. downstream from the orf was an untranslatable region of 189 bp followed by eight bp of polyadenylate. the probable location of the psbmv coat protein codons within the long orf was determined by comparing the inferred amino acid sequence with oth ... | 1990 | 2391503 |
| detection of an unidentified potyvirus from roystonea regia palm using the polymerase chain reaction and degenerate, potyvirus specific, primers and potential problems arising from the amplification of host plant dna sequences. | degenerate potyvirus-specific primers were used in the pcr to amplify cdna representing a 335 nucleotide region of the coat protein gene in rna purified from an uncharacterised potyvirus isolated from roystonea regia palms in queensland, australia. the rna was also detected by pcr in total nucleic acid extracts from infected nicotiana benthamiana, n. clevelandii and vicia faba. the method also successfully detected pea seed-borne mosaic virus in pisum sativum. in addition the procedure amplified ... | 1994 | 7714044 |
| nucleotide sequence of johnsongrass mosaic potyvirus genomic rna. | the complete nucleotide sequence of the rna genome of johnsongrass mosaic virus (jgmv), which infects monocotyledonous plant species, has been determined from cloned viral cdnas. the jgmv genomic rna is 9,766 nucleotides in length, excluding the poly (a) tail, and contains a large open reading frame that codes for a polyprotein of 3,052 amino acids. the open reading frame is flanked by a 5' untranslated region of 135 and a 3' untranslated region of 475 nucleotides. a comparison of the jgmv polyp ... | 1993 | 8150599 |
| a surprising effect of extraction conditions on the mobility of some plant virus coat proteins in sds-page. | the coat protein from purified particles of pea seedborne mosaic potyvirus (psbmv) moves in sds-page with an apparent molecular weight (m(r)) of 36 kda. however, extracts of psbmv infected plants prepared with sds or urea contain psbmv immunoreactive proteins with apparent m(r) 39 kda as well as 36 kda. the low mobility form may be generated from the apparent m(r) 36 kda form by incubating purified psbmv particles with healthy plant sap in the presence of denaturing agents. a similar effect is o ... | 1994 | 8188819 |
| biological and molecular properties of a pathotype p-1 and a pathotype p-4 isolate of pea seed-borne mosaic virus. | two isolates of pea seed-borne mosaic potyvirus, dpd1 and ny, were identified as pathotypes p-1 and p-4, respectively, by their infectivity on pisum sativum l. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. the two isolates differed in several biological characteristics. dpd1 induced transient vein clearing, downward rolling of leaflets and internode shortening on p. sativum, whereas ny only caused a slight growth reduction. dpd1 moved systemically in chenopodium quinoa whe ... | 1996 | 8683223 |
| susceptibility of some pea cultivars to pea seed-borne mosaic virus infection and virus transmission by seeds. | the susceptibility of 19 pea cultivars to pea seed-borne mosaic virus (psbmv) by mechanical inoculation and the virus transmission by seeds were determined. all tested cultivars were highly susceptible to the virus infection. the virus transmission by seeds was found in 1.9% - 32.7%. a correlation between mechanical and vector-mediated infections of pea and virus transmission by seeds is discussed. | 1995 | 8722299 |
| induction of hsp70 and polyubiquitin expression associated with plant virus replication. | by examining the front of virus invasion in immature pea embryos infected with pea seed-borne mosaic virus (psbmv), the selective control of different host genes has been observed. from our observations, the early responses to psbmv replication can be grouped into three classes, inhibited host gene expression, induced host gene expression, and no effect on a normal host function. the expression of two heat-inducible genes encoding hsp70 and polyubiquitin was induced coordinately with the onset o ... | 1996 | 8986804 |
| multiple viral determinants affect seed transmission of pea seedborne mosaic virus in pisum sativum. | two pea seedborne mosaic potyvirus (psbmv) isolates, p-1 dpd1 (p-1), which is highly seed-transmitted, and p-4 ny (p-4), which is rarely seed-transmitted, and chimeras between p-1 and p-4 were analysed to map the viral genetic determinants of seed transmission. infectivity of chimeric viruses was evaluated by inoculating pisum sativum with rna transcribed in vitro from recombinant full-length cdna clones. the chimeric viruses that were used demonstrated that a genomic segment encoding the 49 kda ... | 1996 | 9000110 |
| analysis of the sequence diversity of the p1, hc, p3, nib and cp genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses. | partial sequences from serologically characterized yam mosaic potyvirus (ymv) isolates were determined in conserved (helper-component proteinase, hc; nuclear inclusion b, nib) and variable (first protein, p1; third protein, p3; and coat protein, cp) regions of the potyviral genome in order to investigate the intraspecies molecular diversity of ymv. multiple sequence alignments and pairwise comparisons were used to quantify the sequence polymorphism in these regions. two levels of diversity were ... | 1997 | 9191916 |
| potyvirus genome-linked protein (vpg) determines pea seed-borne mosaic virus pathotype-specific virulence in pisum sativum. | the mechanism of pisum sativum pathotype-specific resistance to pea seed-borne mosaic potyvirus (psbmv) was investigated and the coding region determinant of psbmv virulence was defined. homozygous recessive sbm-1 peas are unable to support replication of psbmv pathotype 1 (p-1), whereas biochemically and serologically related pathotype 4 (p-4) is fully infectious in the sbm-1/sbm-1 genotype. we were unable to detect viral coat protein or rna with double antibody sandwich-enzyme-linked immunosor ... | 1998 | 9450335 |
| a single conserved amino acid in the coat protein gene of pea seed-borne mosaic potyvirus modulates the ability of the virus to move systemically in chenopodium quinoa. | two isolates of pea seed-borne mosaic potyvirus, dpd1 and ny, which both infect pea (pisum sativum) systemically, differ in their ability to move long distance in chenopodium quinoa. dpd1 spreads to uninoculated leaves, whereas ny is restricted to the inoculated leaves. the ny isolate was found to move from cell to cell infecting all parts of the inoculated leaves, including the petiole. the coat protein (cp) coding region was identified as the determinant of long-distance movement. virus chimer ... | 1998 | 9499805 |
| ultrastructural and temporal observations of the potyvirus cylindrical inclusions (cls) show that the cl protein acts transiently in aiding virus movement. | a systematic ultrastructural study across the edge of an advancing infection in pea seed-borne mosaic potyvirus-infected pea cotyledons showed the cylindrical inclusion (ci) protein to exist in transient functional states. initially, the characteristic ci pinwheel inclusion bodies were positioned centrally over the plasmodesmal apertures (including those of plasmodesmata connected to the previously infected cell), in agreement with a proposed role in virus movement (carrington et al., 1998, plan ... | 1998 | 9614878 |
| specificity of resistance to pea seed-borne mosaic potyvirus in transgenic peas expressing the viral replicase (nlb) gene. | transgenic pea lines carrying the replicase (nib) gene of pea seed-borne mosaic potyvirus (psbmv) were generated and used in experiments to determine the effectiveness of induced resistance upon heterologous isolates. three pea lines showed inducible resistance in which an initial infection by the homologous isolate (psbmv-dpd1) was followed by a highly resistant state. resistance was observed in plants in either the homozygous or hemizygous condition and resulted in no overall yield loss despit ... | 1998 | 9880032 |
| a heat shock transcription factor in pea is differentially controlled by heat and virus replication. | since some heat-inducible genes [heat shock (hs) genes] can be induced by virus infection in pea [e.g. hsp70; aranda et al. 1996, proc. natl acad. sci. usa 93, 15289-15293], we have investigated the effect that heat and virus replication may have on the expression of a heat-shock transcription factor gene (hsf). we have characterized what appears to be the only member of the hsf family in pea, pshsfa. similar to hsp70, pshsfa is heat-inducible in vegetative and embryonic tissues, which is concor ... | 1999 | 10571875 |
| pea embryonic tissues show common responses to the replication of a wide range of viruses. | the response of pea embryonic tissues to the replication of a range of different viruses was investigated using in situ hybridization to analyze changes in the expression of two host genes, heat shock protein 70 (hsp70) and lipoxygenase (lox1). excised pea embryos were infected using microprojectile bombardment with a nonseed transmissible strain of pea seed-borne mosaic potyvirus, or with pea early browning tobravirus (pebv), white clover mosaic potexvirus, or beet curly top geminivirus. collec ... | 2000 | 10662627 |
| towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system. | a map for the interactions of the major proteins from potato virus a (pva) and pea seed-borne mosaic virus (psbmv) (members of the genus potyvirus:, family potyviridae:) was generated using the yeast two-hybrid system (yths). interactions were readily detected with five pva protein combinations (hc-hc, hc-ci, vpg-vpg, nia-nib and cp-cp) and weak but reproducible interactions were detected for seven additional combinations (p1-ci, p3-nib, niapro-nib, vpg-nia, vpg-niapro, niapro-nia and nia-nia). ... | 2001 | 11257200 |
| nucleotide sequence and infectious cdna clone of the l1 isolate of pea seed-borne mosaic potyvirus. | the complete nucleotide sequence of pea seed-borne mosaic potyvirus isolate l1 has been determined from cloned virus cdna. the psbmv l1 genome is 9895 nucleotides in length excluding the poly(a) tail. computer analysis of the sequence revealed a single long open reading frame (orf) of 9594 nucleotides. the orf potentially encodes a polyprotein of 3198 amino acids with a deduced mr of 363537. nine putative proteolytic cleavage sites were identified by analogy to consensus sequences and genome arr ... | 2001 | 11266209 |
| mutations in pea seedborne mosaic virus genome-linked protein vpg after pathotype-specific virulence in pisum sativum. | pisum sativum plant introduction (pi) line 269818 is resistant to potyvirus pea seedborne mosaic virus (psbmv) isolates, categorized as pathotype p1, and is susceptible to pathotype p4 isolates. this difference in infectivity is determined by the viral genome-linked protein (vpg) cistron. mutational analysis of vpg of psbmv isolates dpd1 and ny representing pathotypes p1 and p4 revealed that codon changes affecting amino acids 105 to 117 in the central region of vpg influenced virulence on pi 26 ... | 2001 | 11386366 |
| recessive resistance in pisum sativum and potyvirus pathotype resolved in a gene-for-cistron correspondence between host and virus. | pea seed-borne mosaic potyvirus (psbmv) isolates are divided into pathotypes p-1, p-2, and p-4 according to their infection profile on a panel of pisum sativum lines. p. sativum pi 269818 is resistant to p-1 and p-2 isolates and is susceptible to p-4 isolates. resistance to p-1 is inherited as a single recessive gene, denoted sbm-1, and the pathogenicity determinant has previously been mapped to the virus-coded protein vpg. in the cultivar bonneville, a second recessive gene, sbm-2, confers spec ... | 2001 | 11413328 |
| genomic heterogeneity in pea seed-borne mosaic virus isolates from pakistan, the centre of diversity of the host species, pisum sativum. | a range of isolates of pea seed-borne mosaic virus (psbmv) was compared in the segments of the genome representing the partial nib/cp/utr and the partial p1-pro/hc-pro coding regions. nucleotide and amino acid sequences, and a phylogenetic analysis of the cp region, divided isolates with available sequence information into two groups, one representing pathotype 4, the other pathotype 1. the pathotype 1 group showed greater diversity than the pathotype 4 group. a comparison of 14 isolates, s6 (a ... | 2001 | 11722010 |
| a model for seed transmission of a plant virus: genetic and structural analyses of pea embryo invasion by pea seed-borne mosaic virus. | pea seed-borne mosaic virus (psbmv), a seed-transmitted virus in pea and other legumes, invades pea embryos early in development. this process is controlled by maternal genes and, in a cultivar that shows no seed transmission, is prevented through the action of multiple host genes segregating as quantitative trait loci. these genes control the ability of psbmv to spread into and/or multiply in the nonvascular testa tissues, thereby preventing the virus from crossing the boundary between the mate ... | 1994 | 12244258 |
| sequence analysis demonstrates that onion yellow dwarf virus isolates from china contain a p3 region much larger than other potyviruses. | the complete sequence of an isolate of onion yellow dwarf virus (oydv) from yuhang, zhejiang province, china, was determined. it was 10538 nts in length and was predicted to encode a polyprotein 3403 amino acids (aa) long with a calculated m(r) of 385.1 kda. the predicted p3 protein (530 aa) was larger than that of any of the potyviruses sequenced to date (344-378 aa). the additional sequence occurs at the n-terminus of the protein, does not represent a duplication from elsewhere in the oydv gen ... | 2003 | 12756621 |
| pea seed-borne mosaic virus seed transmission exploits novel symplastic pathways to infect the pea embryo and is, in part, dependent upon chance. | seed transmission of pea seed-borne mosaic virus (psbmv) depends upon symplastic transport of the virus from infected maternal cells to the embryo. such transport pathways have not been identified in higher plants. to identify these pathways, we have studied the ultrastructure of the tissues and cells around the micropyle of young developing seeds and compared transmitted and nontransmitted virus isolates. a characteristic of psbmv infection was the presence of cylindrical inclusions positioned ... | 2003 | 14513309 |
| identification of markers tightly linked to sbm recessive genes for resistance to pea seed-borne mosaic virus. | two virus resistance loci on linkage groups ii and vi have provided the only sources of natural resistance against pea seed-borne mosaic virus (psbmv, potyviridae) in the important crop plant pisum sativum l. a combination of parallel approaches was used to collate linked markers, particularly for sbm-1 resistance on linkage group vi. we have identified sequences derived from the genes for the eukaryotic translation initiation factors eif4e and eif(iso)4e as being very tightly linked to the resi ... | 2004 | 15067509 |
| the potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eif4e in cell-to-cell trafficking. | from the characterization of the recessive resistance gene, sbm1, in pea we have identified the eukaryotic translation initiation factor, eif4e, as a susceptibility factor required for infection with the potyvirus, pea seed-borne mosaic virus. a functional analysis of the mode of action of the product of the dominant allele revealed a novel function for eif4e in its support for virus movement from cell-to-cell, in addition to its probable support for viral rna translation, and hence replication. ... | 2004 | 15469495 |
| from orfeomes to protein interaction maps in viruses. | although cloned viral orfeomes are particularly well suited for genome-wide interaction mapping due to the limited size of viral genomes, only a few such studies have been published. here, we summarize virus interaction mapping projects involving vaccinia virus, hepatitis c virus (hcv), potato virus a (pva), pea seed-borne mosaic virus (psbmv), and bacteriophage t7, as well as some projects in progress. the studies reported suggest that virus-specific coding and replication strategies must be ta ... | 2004 | 15489322 |
| first report of occurrence of two viruses on pea field in iran. | an intensive survey was conducted to identify virus diseases affecting pea crops in tehran province of iran. a total of 270 pea samples were collected randomly from pea fields. samples were tested by double antibody sandwich enzyme linked immunosorbent assay (das-elisa) using polyclonal antisera prepared against psbmv (as-0129, dsmz, braunschweig, germany) and tswv (as-0580, dsmz, braunschweig, germany). virus disease incidence in pea samples was followed by psbmv (33%) tswv (24.4%) and psbmv+ts ... | 2005 | 16637207 |
| multiple determinants in the coding region of pea seed-borne mosaic virus p3 are involved in virulence against sbm-2 resistance. | viral determinants for overcoming pisum sativum recessive resistance, sbm-2, against the potyvirus pea seed-borne mosaic virus (psbmv) were identified in the region encoding the n-terminal part of the p3 protein. codons conserved between sbm-2 virulent isolates in this region: q21, k30 and h122 were found to specifically impair sbm-2 virulence when mutated in selected genetic backgrounds. the corresponding amino acids, gln21 and lys30, are neighbored by p3 residues strongly conserved among potyv ... | 2006 | 16908044 |
| occurrence of viruses infecting pea in iran. | a survey was conducted to determine the incidence of alfalfa mosaic virus (amv), bean yellow mosaic virus (bymv), broad bean wilt virus-1 (bbwv), pea leafroll virus (plrv), pea enation mosaic virus (pemv), pea seed borne mosaic virus (psbmv), potato virus x(pvx), tomato mosaic virus (tomv), tomato spotted wilt virus (tswv) on pea (pisum sativum) in iran. a total of 1276 random and 684 symptomatic pea samples were collected during the spring and summer of 2002-2004 in tehran province of iran, whe ... | 2006 | 17390891 |
| inhibition of host gene expression associated with plant virus replication. | pea seed-borne mosaic virus (psbmv) rna replication in pea cotyledonary tissues was restricted largely to a zone of cells close to the infection front. in situ hybridization probes representing nine genes from two pathways of metabolism failed to detect rna transcripts within this zone, although transcripts were found in similar amounts in tissues on either side of the zone. thus, in common with some animal viruses, psbmv transiently suppresses the expression of host genes. host protein accumula ... | 1995 | 17791345 |
| genetic and biological diversity of the pea seed-borne mosaic virus isolates occurring in czech republic. | eight isolates of the pea seed-borne mosaic virus (psbmv) from the czech republic were studied regarding their biological and molecular characteristics. molecular characterization using rt-pcr was done on the 5'(nter)nib-cp-utr3' region amplified using universal cpup/p9502 primer pair and the newly designed psb8812/psb944, and psb8800/psb9440 primer pairs, respectively. sequential and phylogenetic analysis of cp-utr3' region from all isolates showed that the available czech and genbank psbmv iso ... | 2008 | 18459836 |
| the use of green fluorescent protein-tagged recombinant viruses to test lettuce mosaic virus resistance in lettuce. | abstract seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mo1(1) and mo1(2), are the principal control methods for lettuce mosaic virus (lmv) in lettuce. although for a few lmv isolates, mo1(2) confers resistance with most isolates, the genes mo1(1) or mo1(2) confer a tolerance, and virus accumulation is readily detected in mo1-carrying plants. this phenotype complicates evaluation of the resistance status, in particular for mo1(1), for which ... | 2002 | 18943090 |
| genomic heterogeneity and host recovery of isolates of malva vein clearing virus. | malva vein clearing virus (mvcv), a tentative species of the genus potyvirus, was identified as the causal agent of viral symptoms in malva sp. weed plants. amplified viral genomic fragments corresponding to approximately 20% of the 3' terminal region of its genome were obtained using non-species specific, genus-specific reagents. the sequences of the pcr fragments were determined. blast and phylogenetic analyses of the deduced amino acid sequence indicated that mvcv is a distinct species of the ... | 2009 | 19059441 |
| quantifying effects of seedborne inoculum on virus spread, yield losses, and seed infection in the pea seed-borne mosaic virus-field pea pathosystem. | field experiments examined the effects of sowing field pea seed with different amounts of infection with pea seed-borne mosaic virus (psbmv) on virus spread, seed yield, and infection levels in harvested seed. plots were sown with seed with actual or simulated seed transmission rates of 0.3 to 6.5% (2005) or 0.1 to 8% (2006), and spread was by naturally occurring migrant aphids. plants with symptoms and incidence increased with the amount of primary inoculum present. when final incidence reached ... | 2009 | 19740029 |
| sources of target specificity associated with the recovery against pea seed-borne mosaic virus infection mediated by rna silencing in pea. | summary transgenic peas containing a copy of the pea seed-borne mosaic virus (psbmv) isolate dpd1 nib sequence develop a 'recovery' phenotype when challenged with either the homologous (dpd1) or a heterologous (ny) psbmv isolate. however, the specificity of the subsequent resistance differs with respect to the initiation by and targeting of different virus isolates. analysis of tissue in which recovery had been induced by either of the isolates, revealed the presence of low molecular rna molecul ... | 2004 | 20565580 |
| a comparison between virus replication and abiotic stress (heat) as modifiers of host gene expression in pea. | abstract pea embryonic tissues respond to active replication of pea seed-borne mosaic potyvirus (psbmv) by the down-regulation of a range of genes and the induction of others. both of these responses can be seen when tissues are subjected to abiotic stress, particularly heat. we have compared the effects of the two inducers to assess whether the host alterations following virus replication represent generic responses to stress, or more specific effects. five classes of response were identified: ... | 2000 | 20572962 |
| structure-based mutational analysis of eif4e in relation to sbm1 resistance to pea seed-borne mosaic virus in pea. | pea encodes eukaryotic translation initiation factor eif4e (eif4e(s)), which supports the multiplication of pea seed-borne mosaic virus (psbmv). in common with hosts for other potyviruses, some pea lines contain a recessive allele (sbm1) encoding a mutant eif4e (eif4e(r)) that fails to interact functionally with the psbmv avirulence protein, vpg, giving genetic resistance to infection. | 2011 | 21283665 |
| genetic variability of the coat protein sequence of pea seed-borne mosaic virus isolates and the current relationship between phylogenetic placement and resistance groups. | nucleotide sequences of complete or partial coat protein (cp) genes were determined for 11 isolates of pea seed-borne mosaic virus (psbmv) from australia and one from china, and compared with known sequences of 20 other isolates. on phylogenetic analysis, the isolates from australia and china grouped into 2 of 3 clades. clade a contained three sub-clades (ai, aii and aiii), australian isolates were in ai or aiii, and the chinese isolate in aii. clade a contained isolates in pathotypes p-1, p-2 a ... | 2011 | 21519930 |
| narrow bottlenecks affect pea seedborne mosaic virus populations during vertical seed transmission but not during leaf colonization. | the effective size of populations (ne) determines whether selection or genetic drift is the predominant force shaping their genetic structure and evolution. populations having high ne adapt faster, as selection acts more intensely, than populations having low ne, where random effects of genetic drift dominate. estimating ne for various steps of plant virus life cycle has been the focus of several studies in the last decade, but no estimates are available for the vertical transmission of plant vi ... | 2014 | 24415934 |
| proteomics offers insight to the mechanism behind pisum sativum l. response to pea seed-borne mosaic virus (psbmv). | pea seed-borne mosaic virus (psbmv) significantly reduces yields in a broad spectra of legumes. the eukaryotic translation initiation factor has been shown to confer resistance to this pathogen, thus implying that translation and proteome dynamics play a role in resistance. this study presents the results of a proteome-wide analysis of pisum sativum l. response to psbmv infection. lc-ms profiling of two contrasting pea cultivars, resistant (b99) and susceptible (raman) to psbmv infection, detect ... | 2017 | 27235724 |
| linkage mapping of sbm-1, a gene conferring resistance to pea seed-borne mosaic virus, using molecular markers in pisum sativum. | the location of sbm-1 on the pisum sativum genetic map was determined by linkage analysis with eight syntenic molecular markers. analysis of the progeny of two crosses confirmed that sbm-1 is on chromosome 6 and permitted a more detailed map of this chromosome to be constructed. the inclusion of fed-1 and prx-3 among the markers facilitated the comparison of our map with the classical genetic map of pea. the sbm-1 gene is most closely linked to rflp marker gs185, being separated by a distance of ... | 1993 | 24195936 |
| forecasting model for pea seed-borne mosaic virus epidemics in field pea crops in a mediterranean-type environment. | an empirical model was developed to forecast pea seed-borne mosaic virus (psbmv) incidence at a critical phase of the annual growing season to predict yield loss in field pea crops sown under mediterranean-type conditions. the model uses pre-growing season rainfall to calculate an index of aphid abundance in early-august which, in combination with psbmv infection level in seed sown, is used to forecast virus crop incidence. using predicted psbmv crop incidence in early-august and day of sowing, ... | 2017 | 28559099 |
| establishing alighting preferences and species transmission differences for pea seed-borne mosaic virus aphid vectors. | pea seed-borne mosaic virus (psbmv) infection causes a serious disease of field pea (pisum sativum) crops worldwide. the psbmv transmission efficiencies of five aphid species previously found landing in south-west australian pea crops in which psbmv was spreading were studied. with plants of susceptible pea cv. kaspa, the transmission efficiencies of aphis craccivora, myzus persicae, acyrthosiphon kondoi and rhopalosiphum padi were 27%, 26%, 6% and 3%, respectively. lipaphis erysimi did not tran ... | 2017 | 28408208 |