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fungal transmission of a potyvirus: uredospores of puccinia sorghi transmit maize dwarf mosaic virus.maize dwarf mosaic virus (mdmv) and maize rust, puccinia sorghi schw., occur as natural infections on cultivated maize in south africa. p. sorghi often occurs as a secondary late infection on maize plants which have already been infected with mdmv earlier in the season, either seed or aphid transmitted. when mdmv isolates from maize plants naturally infected by both virus and fungus were propagated by sap inoculation in plant growth rooms, residual uredospores in the sap gave rise to the develop ...19921450745
differentiation of the four viruses of the sugarcane mosaic virus subgroup based on cytopathology.a cytological comparison has been made of representative isolates of johnsongrass mosaic (jgmv), maize dwarf mosaic (mdmv), sorghum mosaic (srmv) and sugarcane mosaic (scmv) viruses. these four viruses now encompass the complex of virus strains which were formerly considered as strains of sugarcane mosaic and/or maize dwarf mosaic viruses. the structure of the cytoplasmic cylindrical inclusions induced by these viruses, together with other cytological alterations, allow the four viruses to be di ...19921450762
present status of the sugarcane mosaic subgroup of potyviruses.until recently, sugarcane mosaic virus (scmv) was believed to be a single potyvirus consisting of a large number of strains, differing from each other in certain biological and antigenic properties. the use of affinity-purified polyclonal antibodies directed towards the surface-located, virus-specific amino termini of the coat proteins showed that 17 strains from australia and the united states represented four distinct potyviruses, namely johnsongrass mosaic virus (jgmv), maize dwarf mosaic vir ...19921450763
detection of ribonuclease-resistant rna in chloroplasts of corn leaf tissue infected with maize dwarf mosaic virus. 19744131629
infectivity changes of maize dwarf mosaic virus in vivo and in vitro. 19695377756
inhibition of viral aphid transmission by the n-terminus of the maize dwarf mosaic virus coat protein.since removal of the exposed n-terminus of the coat protein of some potyviruses abolishes aphid transmission, the role of this coat protein region of maize dwarf mosaic potyvirus (mdmv) in aphid transmission was investigated. the viral cdna encoding this region was cloned and expressed as a fusion protein in bacteria. the resulting purified n-terminus of the coat protein was used in controlled aphid transmission experiments in competition with mdmv. the results show that this region inhibits aph ...19957491793
banana bract mosaic virus: characterisation using potyvirus specific degenerate pcr primers.banana bract mosaic (bbmv) is a relatively new, non-persistently aphid transmitted disease of bananas in the philippines. partially purified preparations from infected plants contained low numbers of flexuous virions, 660 to 760 nm in length, and a 38kda protein, possibly the coat protein, which reacted with a general potyvirus antiserum in western blots. there were insufficient virions for conventional antiserum production or cdna synthesis. therefore, dna was amplified using potyvirus-specific ...19957733824
in situ localization of atpase activity in cells of plants infected by maize dwarf mosaic potyvirus.cells of healthy maize plants as well as those infected by maize dwarf mosaic potyvirus were examined by electron microscopy for the location of atpase activity. in healthy and virus infected plants, atpase activity was found in plasma membranes, chloroplast thylakoid membranes, nuclear membranes and in mitochondria. in virus-infected cells, atpase activity was also observed in cytoplasmic vesicles which were found in close proximity to the virus-specific cytoplasmic inclusion bodies (ci), at th ...19948129627
complete nucleotide sequence and analysis of the putative polyprotein of maize dwarf mosaic virus genomic rna (bulgarian isolate).the complete nucleotide sequence of maize dwarf mosaic virus bulgarian isolate (mdmv-bg) was determined. the viral genome was 9515 nt and contained an open reading frame encoding 3042 amino acids, flanked by 3'- and 5'-utrs of 139 and 250 nucleotides, respectively. mdmv-bg was more conserved in the coding region (52.9%) than in the utrs (45.8%) when compared to the 15 other potyviruses. of ten putative gene products of mdmv-bg, the p1 was the most variable protein (24.9%) while the nib was the m ...19989787662
properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria.a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ...19989842442
the complete sequence of the genome of cocksfoot streak virus (csv), a grass infecting potyvirus.the complete nucleotide sequence of cocksfoot streak virus (csv) has been determined. the viral genome comprises 9663 nucleotides, excluding a 3'-terminal poly(a) sequence. the genome of csv has a 133 nt 5'-non coding and a 260 nt 3'-non coding region. the rna of csv encodes a single polyprotein of 3089 amino acid residues and has a deduced genome organization typical for a member of the family potyviridae. csv is transmissible by aphids and has a narrow host range in the gramineae. it was compa ...200212181676
maize dwarf mosaic disease in different regions of china is caused by sugarcane mosaic virus.sugarcane mosaic virus (scmv) was detected in all 62 maize samples collected from eight maize-growing provinces in china showing dwarf mosaic symptoms by immunocapture reverse-transcription polymerase chain reaction (rt-pcr). maize dwarf mosaic virus (mdmv), sorghum mosaic virus (srmv) and johnsongrass mosaic virus (jgmv), however, were not detected in any of the samples by rt-pcr. eleven cdna fragments of approximately 0.8 kilobases covering most of the coat protein (cp) gene of scmv were seque ...200212491109
characterization of a distinct johnsongrass mosaic virus strain isolated from sorghum in nigeria.a virus isolated from sorghum in nigeria has been partially characterized. it was tested by enzyme-linked immunosorbent assay (elisa) using antisera to maize dwarf mosaic virus, johnsongrass mosaic virus (jgmv), sugarcane mosaic virus strain-mdb, sorghum mosaic virus, and zea mosaic virus. a partial host range, symptom phenotypes for selected sorghum lines, and the mass of the coat protein (cp) subunit was analyzed by sodium-dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its ...200515503220
identification of a naturally occurring recombinant isolate of sugarcane mosaic virus causing maize dwarf mosaic disease.the complete nucleotide sequence of a potyvirus causing severe maize dwarf mosaic disease in shaanxi province, northwestern china was determined (genbank accession no. ay569692). the full genome is 9596 nucleotides in length excluding the 3 '-terminal poly (a) sequence. it contains a large open reading frame (orf) flanked by a 149 nt 5'-untranslated region (utr) and a 255 nt 3'-utr. the putative polyprotein encoded by this large orf comprises of 3063 amino acid residues. sequence comparisons and ...200515744565
complete genome sequences of maize dwarf mosaic and sugarcane mosaic virus isolates coinfecting maize in spain.the genomes of spanish isolates of maize dwarf mosaic virus (mdmv-sp) and sugarcane mosaic virus (scmv-sp) were completely sequenced. nucleotide sequence identities of scmv-sp to those of other scmv isolates ranged from 79 to 90%. mdmv-sp shared 85% nucleotide identity with the only other fully sequenced isolate of mdmv. mdmv-sp and scmv-sp differed from each other by 31% in their nucleotide sequences. phylogenetic analyses showed that scmv isolates group by host rather than by geographical loca ...200717680319
characterization of a novel potyvirus isolated from maize in israel.a potyvirus (proposed name of zea mosaic virus [zemv]) isolated from maize in israel was analyzed by serology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of capsid proteins, symptomatology, and sequencing. parts of the nuclear inclusion b, coat protein, and 3' regions were sequenced; the amino acid sequence of zemv capsid was determined by time-of-flight mass spectrometry (tofms). the results of these analyses were compared with those of similar analyses of the followin ...200018944557
impact of 9 years of bt-maize cultivation on the distribution of maize viruses.this study assesses the effect of bt-maize on the distribution of maize viruses. random surveys were conducted in spain between 2001 and 2006 to evaluate the occurrence of maize viruses in bt-maize cultivation areas and in areas where this crop had not been introduced. maize dwarf mosaic virus (mdmv) was the predominant virus in bt-areas, and maize rough dwarf virus (mrdv) was the most predominant one in non-bt-areas, with mrdv an emergent virus in both types of areas. a decline in the occurrenc ...200919067216
application of sequence-independent amplification (sia) for the identification of rna viruses in bioenergy crops.miscanthus x giganteus, energycane, and panicum virgatum (switchgrass) are three potential biomass crops being evaluated for commercial cellulosic ethanol production. viral diseases are potentially significant threats to these crops. therefore, identification of viruses infecting these bioenergy crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. the application is described of sequence-independent amplification, for the identif ...201020638415
serological relationships between the cylindrical inclusion proteins of potyviruses.abstract antisera to the cytoplasmic inclusion proteins (cips) of bean yellow mosaic (bymv), clover yellow vein (clyvv), turnip mosaic (tumv), sweet potato feathery mottle (spfmv), and maize dwarf mosaic (mdmv) potyviruses were used to examine the relationships between the cips of 18 potyviruses. the antisera to cips of bymv, clyvv, tumv, and spfmv cross-reacted to most or all of the purified cips tested in western blot assays. the mdmv cip antiserum reacted significantly only to the mdmv and so ...199818944875
the complete sequence of a sugarcane mosaic virus isolate causing maize dwarf mosaic disease in china.the complete sequence of a potyvirus from maize in zhejiang province was determined. the rna was 9596 nucleotides long, excluding the 3'-poly (a) tail, and there was a single long open reading frame (orf) of 9192 nts encoding a 346.1 ku polyprotein. the polyprotein had substantial amino acid sequence homology with those encoded by the rnas of a chinese isolate of sorghum mosaic virus (srmv-c) and a bulgarian isolate of maize dwarf mosaic virus, but it was most closely related to sugarcane mosaic ...200218759055
iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences.iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ...201727632283
topographic analysis of tobacco etch virus capsid protein epitopes.monoclonal antibodies have been prepared, which react with capsid protein of an aphid-transmitted isolate of tobacco etch virus (tev). ten different monoclonal antibodies were characterized with reference to (1) antibody class, (2) reactivity with different plant virus antigens, (3) the spatial relationship between epitopes, and (4) whether these epitopes were located on the exterior surface of the virion. three monoclonal antibodies were specific for tev isolates. these monoclonal antibodies re ...198518640514
the genomic sequence and biological properties of pennisetum mosaic virus, a novel monocot-infecting potyvirus.the complete nucleotide sequences of two isolates of pennisetum mosaic virus (penmv) were determined. the viral genome comprised 9,611 nucleotides (nt) excluding the 3'-terminal poly(a) sequence, with the capacity of encoding a single polyprotein of 3,065 amino acids. the large open reading frame is flanked by a 172-nt 5'-untranslated region (utr) and a 244-nt 3'-utr. sequence comparisons and phylogenetic analyses of the complete genome and polyproteins suggest that penmv is closely related to o ...200818365128
identification of prevalent potyvirus on maize and johnsongrass in corn fields of tehran province of iran and a study on some of its properties.during a growing season in 2004, 231 leaf samples of virus infected and mosaic and dwarf mosaic symptoms showing maize (zea mays l.) plants and 258 leaf samples of mosaic showing johnsongrass (sorghum halepens l.) plants from various corn fields in tehran province were collected. serological tests of das-elisa and diba were performed on samples using antisera of sugarcane mosaic virus (scmv), maize dwarf mosaic virus (mdmv), sorghum mosaic virus (srmv) and johnsongrasss mosaic virus (jgmv). in b ...200617390895
the effects of b, k10, and ar chromosomes on the resistance of maize to viral infection.studies were conducted to determine if accessory (b) chromosomes, the abnormal tenth (k10) chromosome or the aberrant ratio (ar) phenomenon of maize (zea mays l.) affect the resistance of the plants to viral infection. genetically similar stocks of maize with and without these elements were compared to determine what effect they would have on the plants response to brome mosaic virus (bmv), maize dwarf mosaic virus (mdmv), wheat streak mosaic virus (wsmv) and barley stripe mosaic virus (bsmv).-t ...197817248865
complete sequence and development of a full-length infectious clone of an ohio isolate of maize dwarf mosaic virus (mdmv).maize dwarf mosaic virus (mdmv) is an important and widespread aphid-transmitted virus of maize. it is a member of the genus potyvirus in the family potyviridae with a monopartite (+) ssrna genome. here we report the complete genome sequence and construction and testing of infectious clones of an ohio isolate of mdmv. full-length mdmv cdna was cloned into the vector psport. full-length cdna pcr-amplified from the vector constructs were used as template for in vitro transcription, and transcripts ...201222342423
co-segregation of the maize dwarf mosaic virus resistance gene, mdm1, with the nucleolus organizer region in maize.the mdm1 locus on the short arm of chromosome six confers resistance in maize to five strains of the maize dwarf mosaic virus (mdmv), an aphid transmitted potyvirus. the location of mdm1 in relation to rflp and morphological loci on the short arm of chromosome six was determined using bc1 and f2 mapping populations. the following map order and distance in cm was obtained from the f2 population; jc1270-2.5-npi245-1.6-umc85/po1-0.5-mdm1/nor-0.5-bnl6.29a-0.5-npi235-0.8-npi101a-4.3-numc59. no recomb ...199524173923
analysis of sugarcane mosaic virus resistance in maize in an isogenic dihybrid crossing scheme and implications for breeding potyvirus-resistant maize hybrids.the gene action of 2 sugarcane mosaic virus (scmv) resistance loci in maize, scmv1 and scmv2, was evaluated for potyvirus resistance in an isogenic background. all 4 homozygous and 5 heterozygous isogenic genotypes were produced for introgressions of the resistant donor (fap1360a) alleles at both loci into the susceptible parent (f7) genetic background using simple sequence repeat markers. for scmv and maize dwarf mosaic virus (mdmv), virus symptoms appeared rapidly in the 3 homozygous genotypes ...200617213909
[isolation of the capsid protein gene of maize dwarf mosaic virus and its transformation in maize].the mdmv (maize dwarf mosaic virus, mdmv) cp (coat protein, cp) gene was cloned by rt-pcr method and introduced into the embryonic calli derived from immature embryos of elite inbred 18-599hong and 18-599bai via particle bombardment. bombarded calli were selected on selection medium containing 5-10 mg/l (ppt) bialaphos. from resistant calli, 79 plantlets were regenerated. 18 of 79 were grown and harvested. the results of southern blotting and pcr analysis demonstrated that mdmv cp have been inte ...200515859345
complete nucleotide sequence of a potyvirus causing maize dwarf mosaic disease in central china.the full-length nucleotide sequence of a potyvirus causing the maize dwarf mosaic (mdm) disease in henan province, central china, was obtained by reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of the cdna 5'-end (5'-race). the viral genome comprised of 9596 nucleotides except the polya tail and encoded a putative polyprotein of 3603 amino acids. the entire genomic sequence of this isolate shared identities of 94.2% and 98.3% with sugarcane mosaic virus (scmv) hz ...200315068377
molecular characterization of a distinct potyvirus from whitegrass in china.apotyvirus isolated from perennial whitegrass ( pennisetum centrasiaticum tzvel.) in north china was characterized at the molecular level. the 3' terminal nucleotide (nt) sequence of 1669 nt of the viral rna genome has been determined, which covered the coding region of the c-terminal part of the large nuclear inclusion protein (nib, rna polymerase), capsid protein (cp) gene and the 3' nontranslated region (ntr). the cp gene consisted of 909 nt (including the stop codon) encoding 302 amino acid ...200312756626
identification and molecular tagging of two complementary dominant resistance genes to maize dwarf mosaic virus.maize dwarf mosaic is one of the devastating and widespread viral diseases in the world. so far, only a few genes were identified and mapped in the resistant materials. a new resistant elite inbred line siyi was identified with resistance to maize dwarf mosaic virus strain b at early and adult stage. two complementary dominant genes conditioned the resistance, with a new genetic model, of the maize inbred line were found at adult stage by the genetic analysis based on parents, f1, f2 and backcro ...200212693101
complete sequence of the genomic rna of the prevalent strain of a potyvirus infecting maize in china.the complete nucleotide sequence of the prevalent strain of a potyvirus isolated from maize in beijing, china was determined and compared with other closely related potyviruses. the viral genome comprises 9595 nucleotides, excluding the poly (a) tail, and encodes a putative polyprotein of 3063 amino acid residues. sequence comparison of the coat proteins showed that this isolate was most closely related to most other potyviral isolates infecting maize across china with identities of about 99% an ...200312664299
cytological modifications in maize plants infected by barley yellow dwarf virus and maize dwarf mosaic virus.three inbred lines of maize (33-16, mo17 and b73) differing in their susceptibility to barley yellow dwarf virus and maize dwarf mosaic virus were studied to compare the ultrastructural modifications induced by the two viruses in leaf tissues of different age. the results demonstrate that the alterations induced by the two viruses in the different maize lines could depend on the particular line tested.200212475565
characterisation of potyviruses from sugarcane and maize in china.sugarcane or maize leaves with mosaic virus symptoms were collected from 13 sites in china. sequence data showed that all 8 samples from maize contained sugarcane mosaic virus (scmv); complete sequences were determined from 2 samples and partial sequences (the ci coding region and the 3'-part of the genome) from the others. the 5 sugarcane samples all contained a virus tentatively described as sorghum mosaic virus (srmv) and in three of them scmv was also detected; 2 srmv sequences and the 3 scm ...200212111432
transmission of viral rna and dna to maize kernels by vascular puncture inoculation.vascular puncture inoculation (vpi) is an effective technique for transmission of maize viruses without using arthropods or other biological vectors. it involves using a jeweler's engraving tool to push minuten pins through a droplet of virus inoculum toward the major vascular bundle in the scutellum of germinating kernels. here, vpi is shown to be useful for introducing rna and dna viral genomes into maize. maize dwarf mosaic potyvirus (mdmv) virions, mdmv genomic rna, foxtail mosaic potexvirus ...200111576640
nucleotide sequence of maize dwarf mosaic virus capsid protein gene and its expression in escherichia coli.the 3'-terminal 1,279 nucleotide sequence of maize dwarf mosaic virus (mdmv) genome has been determined. this sequence contains an open reading frame of 1,023 nucleotides and a 3'-non-coding region of 256 nucleotides. the open reading frame includes all of the coding regions for the viral capsid protein (cp) and part of the viral nuclear inclusion protein (nib). the predicted viral cp consists of 313 amino acid residues with a calculated molecular weight of 35,400. the amino acid sequence of the ...19957766313
transgenic corn plants expressing mdmv strain b coat protein are resistant to mixed infections of maize dwarf mosaic virus and maize chlorotic mottle virus.the maize dwarf mosaic virus strain b (mdmv-b) coat protein (cp) gene was cloned into a monocot expression cassette and introduced into sweet corn cell suspension cultures via particle bombardment or electroporation. transformed cells were selected on culture media containing 300 mg/l kanamycin, and plants were regenerated. cells from all transformed lines expressed the cp gene; and one transgenic line synthesized approximately 100-200 micrograms mdmv-cp per gram fresh weight. plants regenerated ...19937764246
production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay.hybridomas were developed that secreted antibodies to soybean mosaic (smv), lettuce mosaic (lmv) viruses, and to maize dwarf mosaic (mdmv) virus, strains ap and b. all hybridomas produced antibodies specific to the homologous virus except for one to lmv, which produced antibodies that reacted at a low level with smv and mdmv. monoclonal antibodies against smv were used in a double-antibody sandwich radioimmunoassay. the assay using only one type of monoclonal antibody lacked sensitivity because, ...19846699619
cylindrical inclusion bodies of wheat streak mosaic virus and three other potyviruses only self-assemble in mixed infections.potyviruses produce cylindrical inclusions (cis) in the cytoplasm of infected cells. immunogold labelling and electron microscopy of embedded and sectioned wheat and maize cells doubly infected by different potyviruses revealed no mixing of inclusion proteins in cis. the viruses were wheat streak mosaic virus (wsmv), agropyron mosaic virus and hordeum mosaic virus, in wheat, and wsmv and maize dwarf mosaic virus in maize. the three viruses in wheat were indistinguishable morphologically and in u ...19912005428
unexpected sequence diversity in the amino-terminal ends of the coat proteins of strains of sugarcane mosaic virus.the sequence of the 3'-terminal 1343 nucleotides of the sc strain of the sugarcane mosaic virus (scmv-sc) genome was compared with the 1376 nucleotides at the 3' terminus of maize dwarf mosaic virus b (mdmv-b). the scmv-sc sequence includes an open reading frame which codes for the viral coat protein of 313 amino acids (nucleotides 157 to 1116), followed by a 3' non-coding region of 235 nucleotides and a poly(a) tail. the mdmv-b sequence codes for the capsid protein (nucleotides 157 to 1139) of ...19911993866
improvement of resistance to maize dwarf mosaic virus mediated by transgenic rna interference.to overcome the low efficiency of agronomic protection from maize dwarf mosaic disease, susceptible maize inbred line was transformed by agrobacterium tumefaciens harboring hprna expression vectors containing inverted-repeat sequences of different lengths targeting coat protein gene (cp) of maize dwarf mosaic virus (mdmv). after pcr screening and southern blotting, the flanking sequences of the integration sites were amplified by thermal asymmetric interlaced pcr (tail-pcr) and used for analysis ...201121504770
influence of the maize dwarf mosaic virns and simazine upon the buffer soluble proteins and catalase activity of a virus-susceptible and a virus-tolerant variety of zea mays.inbred corn varieties differing in their tolerance to the maize dwarf mosaic virus infection also differ in varietal character and host response to simazine. oh 7b, a tolerant cv., has less total soluble and globulin proteins than the sensitive variety, h 55, but more kjeldahl nitrogen, albumin protein and a doubling of catalase activity. virus inoculation in oh 7b causes a reduction in all three protein fractions, kjeldahl nitrogen and catalase activity. deleterious virus-simazine interaction d ...196920925662
genetic diversity of hungarian maize dwarf mosaic virus isolates.the genetic diversity of the coat-protein (cp) region and the untranslated c-terminal region (3'utr) of maize dwarf mosaic virus (mdmv) was analyzed to evaluate the variability between isolates (inter-isolate sequence diversity). the results of inter-isolate sequence diversity analysis showed that the diversity of the mdmv cp gene is fairly high (p-distance: up to 0.136). during sequence analysis, a 13 amino-acid residue insertion and an 8 amino-acid residue deletion were found within the n-term ...201020033839
natural insertions within the n-terminal region of the coat protein of maize dwarf mosaic potyvirus (mdmv) have an effect on the rna stability.a 13 amino acid residue insertion was found in the n-terminal region of the coat protein of several maize dwarf mosaic virus isolates (mdmv). these insertions seem to be the result of a direct duplication event, but differ in some positions. in order to evaluate the influence of the insertion on the rna secondary structure and stability, the rna secondary structures and minimum free energies (mfe) of all existing mdmv coat protein sequences were estimated using three different softwares, the vie ...201019937270
validation of candidate genes putatively associated with resistance to scmv and mdmv in maize (zea mays l.) by expression profiling.the potyviruses sugarcane mosaic virus (scmv) and maize dwarf mosaic virus (mdmv) are major pathogens of maize worldwide. two loci, scmv1 and scmv2, have ealier been shown to confer complete resistance to scmv. custom-made microarrays containing previously identified scmv resistance candidate genes and resistance gene analogs were utilised to investigate and validate gene expression and expression patterns of isogenic lines under pathogen infection in order to obtain information about the molecu ...200919187556
responses of maize (zea mays l.) near isogenic lines carrying wsm1, wsm2, and wsm3 to three viruses in the potyviridae.genes on chromosomes six (wsm1), three (wsm2) and ten (wsm3) in the maize (zea mays l.) inbred line pa405 control resistance to wheat streak mosaic virus (wsmv), and the same or closely linked genes control resistance to maize dwarf mosaic virus (mdmv) and sugarcane mosaic virus (scmv). near isogenic lines (nil) carrying one or two of the genes were developed by introgressing regions of the respective chromosomes into the susceptible line oh28 and tested for their responses to wsmv, mdmv, and sc ...201121667271
recombination analysis of maize dwarf mosaic virus (mdmv) in the sugarcane mosaic virus (scmv) subgroup of potyviruses.recombination among rna viruses is a natural phenomenon that appears to have played a significant role in the species development and the evolution of many strains. it also has particular significance for the risk assessment of plants which have been genetically modified for disease resistance by incorporating viral sequences into their genomes. however, the exact recombination events taking place in viral genomes are not investigated in detail for many virus groups. in this analysis, different ...201525392089
viruses in maize and johnsongrass in southern ohio.the two major u.s. maize viruses, maize dwarf mosaic virus (mdmv) and maize chlorotic dwarf virus (mcdv), emerged in southern ohio and surrounding regions in the 1960s and caused significant losses. planting resistant varieties and changing cultural practices has dramatically reduced virus impact in subsequent decades. current information on the distribution, diversity, and impact of known and potential u.s. maize disease-causing viruses is lacking. to assess the current reservoir of viruses pre ...201424918609
genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line.novel and previously known resistance loci for six phylogenetically diverse viruses were tightly clustered on chromosomes 2, 3, 6 and 10 in the multiply virus-resistant maize inbred line, oh1vi. virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. genetic resistance to different viruses has been characterized in maize populations in diverse environments using different screening techniques, and resistance loci hav ...201424500307
the population genetics of maize dwarf mosaic virus in spain.the population genetics of maize dwarf mosaic virus (mdmv) in spain was assessed by analysis of the p1-hc region. restriction fragment length polymorphism analysis of 363 isolates revealed that the mdmv population consisted of 69 haplotypes. sequence analysis of 112 isolates confirmed a high degree of nucleotide sequence diversity (0.143), which was higher for p1 than for the hc. twelve sequences showed a single different recombination event. selection pressure analysis revealed that the p1-hc r ...201222855126
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