east african strains of cowpea aphid-borne mosaic virus. 19734701692
cowpea aphid borne mosaic virus-morocco and south african passiflora virus are strains of the same potyvirus.high performance liquid chromatography (hplc) profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphid-borne mosaic virus (cabmv). some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus (b1cmv) while other reports have concluded that this relationship is distant. in this report a tryptic digest of the coat protein of cabmv-morocco was compared w ...19948002788
molecular characterization and interviral homologies of a potyvirus infecting sesame (sesamum indicum) in georgia.phylogenetic analyses of the 3' terminal region of a potyvirus naturally infecting sesame (sesamum indicum) in georgia indicated that the virus belongs to the passionfruit woodiness potyvirus subgroup, and is most closely related to cowpea aphid-borne mosaic potyvirus (cabmv) and south african passiflora potyvirus. based on the sequence identity, this virus is a strain of cabmv. use of degenerate primers, reverse transcription-polymerase chain reaction, cloning and sequence analysis allowed us t ...19979672651
phylogenetic analysis of two potyvirus pathogens of commercial cowpea lines: implications for obtaining pathogen-derived a prelude to developing engineered resistance to two important potyvirus pathogens of cowpea, a phylogenetic analysis of strains of cowpea aphid-borne mosaic virus (cabmv) and bean common mosaic virus--blackeye cowpea strain (bcmv-b1c) was undertaken. nucleotide sequences for the coat protein genes and 3'-untranslated regions of four cabmv and one bcmv-b1c strains were determined and included in an analysis with published sequences. while all the newly sequenced viruses showed strong homology ...200010766309
properties of a virus isolated from vernonia amygdalina del. in lagos, nigeria.a previously uncharacterized virus tentatively named vernonia green vein-banding virus (vgvbv) was isolated from vernonia amygdalina del. ("bitterleaf") from lagos, nigeria. the virus was mechanically transmissible but had a narrow host range restricted to nicotiana benthamiana, chenopodium quinoa and c. amaranticolor. it was also transmissible in a non-persistent manner by myzus persicae. the virus was purified from n. benthamiana and about 750 nm long flexuous rod-shaped particles were observe ...200415745049
introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (cabmv).the introduction of anti-apoptotic genes into plants leads to resistance to environmental stress and broad-spectrum disease resistance. the anti-apoptotic gene (p35) from a baculovirus was introduced into the genome of passion fruit plants by biobalistics. eleven regenerated plants showed the presence of the p35 gene by pcr and/or dot blot hybridization. transcriptional analysis of regenerated plants showed the presence of specific p35 transcripts in 9 of them. regenerated plants containing the ...200717016672
interactions of viruses in cowpea: effects on growth and yield parameters.the study was carried out to investigate the effects of inoculating three cowpea cultivars: "olo ii", "oloyin" and it86d-719 with three unrelated viruses: cowpea aphid-borne mosaic virus (cabmv), genus potyvirus, cowpea mottle virus (cmev), genus carmovirus and southern bean mosaic virus (sbmv), genus sobemovirus singly and in mixture on growth and yield of cultivars at 10 and 30 days after planting (dap). generally, the growth and yield of the buffer inoculated control plants were significantly ...200717286870
cowpea viruses: effect of single and mixed infections on symptomatology and virus concentration.natural multiple viral infections of cultivated cowpeas have been reported in nigeria. in this study, three nigerian commercial cowpea cultivars ("olo 11", "oloyin" and "white") and two lines from the iita (it86d- 719 and tvu 76) were mechanically inoculated with cowpea aphid-borne mosaic virus (cabmv), bean southern mosaic virus (sbmv) and cowpea mottle virus (cmev) singly, as well as in all possible combinations at 10, 20 and 30 days after planting (dap). samples of leaves or stems were collec ...200717900355
effect of cowpea seeds contamination rate by the cowpea aphid borne mosaic virus on epidemics development.cowpea aphid borne mosaic virus (cabmv) diseased seeds provide at seedling, virus infected plants which are the only source of primary inoculum. secondary infections are bequeathed by aphids. the objective of this research is to study the development of the secondary infection in field. therefore, eight cowpea varieties with different seed contamination rate (0, 0.05, 0.25, 0.5, 1, 5%) were used over consecutive four years. the infected plants were recorded every week from the tenth day after so ...200719093498
indigenous and introduced potyviruses of legumes and passiflora spp. from australia: biological properties and comparison of coat protein nucleotide sequences.five australian potyviruses, passion fruit woodiness virus (pwv), passiflora mosaic virus (pamv), passiflora virus y, clitoria chlorosis virus (clcv) and hardenbergia mosaic virus (harmv), and two introduced potyviruses, bean common mosaic virus (bcmv) and cowpea aphid-borne mosaic virus (cabmv), were detected in nine wild or cultivated passiflora and legume species growing in tropical, subtropical or mediterranean climatic regions of western australia. when clcv (1), pamv (1), pavy (8) and pwv ...201121744001
inheritance of resistance to potato y viruses in phaseolus vulgaris l : 1. two independent genes for resistance to watermelon mosaic virus-2.resistance to watermelon mosaic virus-2 in phaseolus vulgaris l. is conferred by two distinct dominant alleles at independent loci. based on segregation data one locus is designated wmv, the other, hsw. the dominant allele wmv from cv. great northern 1140 prevents systemic spread of the virus but viral replication occurs in inoculated tissue. in contrast, hsw confers both local and systemic resistance to wmv-2 below 30c. at higher temperatures, plants that carry this allele in the absence of mod ...198724240215
analysis of genome comparison of two indian isolates of cowpea aphid-borne mosaic virus from india.the complete sequence of two cowpea aphid-borne mosaic virus (cabmv) isolates (rr3 and rr4) from india was determined. phylogenetic analysis showed that both isolates showed different closeness with other isolates of cabmv. cabmv-rr3 showed maximum identity of 99 % with cabmv-br1 from brazil at nucleotide and protein levels, whereas cabmv-rr4 showed identity of 73 and 95 % with cabmv-z isolate from zimbabwe at nucleotide and protein levels respectively. similarity identity matrix revealed 69 % i ...201526184969
a distinct tymovirus infecting cassia hoffmannseggii in brazil.leaves of cassia hoffmannseggii, a wild fabaceous species found in the atlantic forest, with a severe mosaic symptom were collected in pernambuco state, brazil. by transmission electron microscopy, two types of virus particles were found: the first was recognized as particles of a potyvirus, which was later identified as cowpea aphid-borne mosaic virus; and the second was isometric and present in high concentration. the observation of vesicles at the periphery of chloroplasts suggested a tymovir ...201222528644
inheritance of resistance to potyviruses in phaseolus vulgaris l. iii. cosegregation of phenotypically similar dominant responses to nine potyviruses.we have identified monogenic dominant resistance to azuki bean mosaic poty virus (azmv), passionfruit woodiness potyvirus-k (pwv-k), zucchini yellow mosaic potyvirus (zymv), and a dominant factor that conditioned lethal necrosis to thailand passiflora potyvirus (thpv), in phaseolus vulgaris 'black turtle soup 1'. resistance to azmv, pwv-k, zymv, watermelon mosaic potyvirus, cowpea aphid-borne mosaic potyvirus, blackeye cowpea mosaic potyvirus, and lethal necrosis to soybean mosaic potyvirus and ...199424178089
survey of aphid population in a yellow passion fruit crop and its relationship on the spread cowpea aphid-borne mosaic virus in a subtropical region of brazil.passion fruit woodiness may be caused by cowpea aphid-borne mosaic virus (cabmv) and is currently the major passion fruit disease in brazil. to assess the virus-vector-host interactions, a newly introduced golden passion fruit plantation located in eastern region of são paulo state, brazil, was monitored.201526413443
additive interactions of unrelated viruses in mixed infections of cowpea (vigna unguiculata l. walp).this study was carried out to determine the effects of single infections and co-infections of three unrelated viruses on three cowpea cultivars (one commercial cowpea cultivar "white" and 2 iita lines; it81d-985 and tvu 76). the plants were inoculated with cowpea aphid-borne mosaic virus (cabmv), genus potyvirus, cowpea mottle virus (cmev), genus carmovirus and southern bean mosaic virus (sbmv), genus sobemovirus singly and in mixture (double and triple) at 10, 20, and 30 days after planting (da ...201526483824
metagenomic-based screening and molecular characterization of cowpea-infecting viruses in burkina faso.cowpea, (vigna unguiculata l. (walp)) is an annual tropical grain legume. often referred to as "poor man's meat", cowpea is one of the most important subsistence legumes cultivated in west africa due to the high protein content of its seeds. however, african cowpea production can be seriously constrained by viral diseases that reduce yields. while twelve cowpea-infecting viruses have been reported from africa, only three of these have so-far been reported from burkina faso. here we use a virion- ...201627764211
inheritance of resistance to potyviruses in phaseolus vulgaris l. iv. inheritance, linkage relations, and environmental effects on systemic resistance to four potyviruses.we have examined the genetics of systemic resistance in phaseolus vulgaris to azuki bean mosaic virus (azmv) and cowpea aphid-borne mosaic virus (cabmv) and the relationship of this resistance to a phenotypically similar resistance to watermelon mosaic virus (wmv) and soybean mosaic virus (smv). in p. vulgaris cv 'great northern 1140' (gn1140), resistance to smv and wmv has been attributed to the genes smv and wmv, respectively, which have been shown to segregate as a unit. systemic resistance t ...199624166169
rna viruses and their silencing suppressors boost abutilon mosaic virus, but not the old world tomato yellow leaf curl sardinia virus.mixed viral infections can induce different changes in symptom development, genome accumulation and tissue tropism. these issues were investigated for two phloem-limited begomoviruses, abutilon mosaic virus (abmv) and tomato yellow leaf curl sardinia virus (tylcsv) in nicotiana benthamiana plants doubly infected by either the potyvirus cowpea aphid-borne mosaic virus (cabmv) or the tombusvirus artichoke mottled crinkle virus (amcv). both rna viruses induced an increase of the amount of abmv, led ...201121843560
comparative analysis of the genomes of two isolates of cowpea aphid-borne mosaic virus (cabmv) obtained from different hosts.the complete genomic sequences of two cowpea aphid-borne mosaic virus (cabmv) isolates from brazil, mg-avr from passion fruit (which also infects cowpea), and br1 from peanut (which also infects cowpea, but not passion fruit), were determined. their nucleotide sequences are 89% identical and display 85% identity to that of cabmv-z. both isolates have the typical potyvirus genome features. p3 and vpg are the most conserved proteins, with 99% amino acid sequence identity between the two isolates, ...201121409445
detection of a resistance gradient to passion fruit woodiness virus and selection of 'yellow' passion fruit plants under field conditions.productivity of 'yellow' passion fruit (passiflora edulis sims. f. flavicarpa o. deg.) is reduced by infection with cowpea aphid-borne mosaic virus (cabmv). we examined resistance in 72 yellow passion fruit plants grown from open-pollinated commercial seed. plants were mechanically inoculated with cabmv virus and maintained in the field in order to select contrasting genotypes for resistance. isolates were obtained from symptomatic leaves of yellow passion fruit plants from field production in l ...200819048500
the prehistory of potyviruses: their initial radiation was during the dawn of agriculture.potyviruses are found world wide, are spread by probing aphids and cause considerable crop damage. potyvirus is one of the two largest plant virus genera and contains about 15% of all named plant virus species. when and why did the potyviruses become so numerous? here we answer the first question and discuss the other.200818575612
a mosaic of beach bean (canavalia rosea) caused by an isolate of cowpea aphid-borne mosaic virus (cabmv) in brazil.beach bean (canavalia rosea) plants showing mosaic symptoms were found at massaguaçú beach, caraguatatuba, brazil. a potyvirus was found to be responsible for the symptoms, based on transmission assays and electron microscopy. a positive reaction in elisa was obtained against cowpea aphid-borne mosaic (cabmv) antisera. viral identity was confirmed by rt-pcr using specific primers to amplify part of the nib and the entire cp coding region of the genome and the 3'ntr. comparison of the amplified s ...200818273677
cowpea aphid-borne mosaic virus (cabmv) is widespread in passionfruit in brazil and causes passionfruit woodiness disease.leaf samples of yellow passionfruit (passiflora edulis f. flavicarpa) displaying fruit woodiness symptoms were collected in seven brazilian states and the federal district. viral infection was confirmed by host range and elisa, and fourteen viral isolates were obtained. all isolates were capable of infecting several leguminous host species, although differences in symptom severity were noticeable. woodiness symptoms were reproduced in yellow passionfruit, and mosaic symptoms were induced in comm ...200616596328
the potyvirus associated with the dappled fruit of passiflora edulis in kagoshima prefecture, japan is the third strain of the proposed new species east asian passiflora virus (eapv) phylogenetically distinguished from strains of passion fruit woodiness virus.a potyvirus (isolate ib) causing dappled or faded fruits and foliar mosaic symptoms of purple passionfruit, was found in the botanical garden of kagoshima university, japan. this isolate--differed in host range from isolates of passion fruit woodiness virus (pwv)-ao, previously reported to cause "woodiness" in japan. isolates ib and ao had 83% amino acid identity in their coat proteins (cps). in phylogenetic analysis, east asian isolates ib, ao, and pwv-taiwan clustered together, and were distin ...200616328141
subcellular location of the helper component-proteinase of cowpea aphid-borne mosaic virus.the helper component-proteinase (hc-pro) of cowpea aphid-borne mosaic virus (cabmv) was expressed in escherichia coli and used to obtain hc-pro antiserum that was used as an analytical tool for hc-pro studies. the antiserum was used in immunofluorescence assays to study the subcellular location of hc-pro expressed with other viral proteins in cowpea protoplasts in a natural cabmv infection, or in protoplasts transfected with a transient expression construct expressing hc-pro separately from othe ...200212416684
transgenic plants expressing hc-pro show enhanced virus sensitivity while silencing of the transgene results in resistance.nicotiana benthamiana plants were engineered to express sequences of the helper component-proteinase (hc-pro) of cowpea aphid-borne mosaic potyvirus (cabmv). the sensitivity of the transgenic plants to infection with parental and heterologous viruses was studied. the lines expressing hc-pro showed enhanced symptoms after infection with the parental cabmv isolate and also after infection with a heterologous potyvirus, potato virus y (pvy) and a comovirus, cowpea mosaic virus (cpmv). on the other ...200212206307
the genomic sequence of cowpea aphid-borne mosaic virus and its similarities with other potyviruses.the genomic sequence of a zimbabwe isolate of cowpea aphid-borne mosaic virus (cabmv-z) was determined by sequencing overlapping viral cdna clones generated by rt-pcr using degenerate and/or specific primers. the sequence is 9465 nucleotides in length excluding the 3' terminal poly (a) tail and contains a single open reading frame (orf) of 9159 nucleotides encoding a large polyprotein of 3,053 amino acids and predicted mr of 348. the size of the genome and the encoded polyprotein is in agreement ...200212021872
molecular characterisation and evolutionary relationships of a potyvirus infecting crotalaria in brazil. brief report.the 3' terminal genomic region of a potyvirus causing mosaic disease in several crotalaria species has been cloned and sequenced. comparisons of the nucleotide and deduced amino acid (aa) sequences of the cloned cdna with those from other potyviruses show that the crotalaria-infecting virus (designated crotalaria mosaic virus; crmv) is closely related to cowpea aphid-borne mosaic virus (cabmv). maximum identity (95.4%) at the coat protein (cp) aa level was observed between crmv and a brazilian s ...200211890532
sequence of the 3'-terminal region of a zimbabwe isolate of cowpea aphid-borne mosaic virus (cabmv).the 3'-terminal 1221 nucleotides of a zimbabwe isolate of cowpea aphid-borne mosaic potyvirus (cabmv) genome have been sequenced. the sequence comprises an open reading frame (orf) of 990 nucleotides and a 3' non-coding-region of 231 nucleotides followed by a poly-a. the orf has high similarity to nib and coat proteins (cp) of potyviruses. a potential cp q/s cleavage site was identified, yielding a cp of 30.5 kda containing 275 amino acids. the cabmv sequence is closely related to that of south ...19968678838
improved diagnosis of cowpea aphid-borne mosaic virus in africa: significance for cowpea seed-indexing, breeding programs and potyvirus taxonomy.large-scale surveys in africa for blackeye cowpea mosaic (b1cmv) and cowpea aphid-borne mosaic (cabmv) showed that several cabmv isolates from southern africa were either not or poorly recognized by monoclonal antibodies prepared to isolates collected in west africa. selection of three new monoclonal antibodies prepared against the maputo (mozambique) isolate of cabmv, and their incorporation into a revised panel of monoclonal antibodies, resulted in the assignment of four of these new cabmv iso ...19968629941
evidence that cowpea aphid-borne mosaic and blackeye cowpea mosaic viruses are two different potyviruses.the immunoreactivity of a panel of polyclonal antibodies and monoclonal antibodies (mabs) raised against african isolates of potyviruses from cowpea and african yam bean was examined in elisas. a serological study including reference isolates followed by further characterization in differential hosts resulted in separation of the potyviruses into two distinct serogroups, one containing blackeye cowpea mosaic virus (blcmv) and the other containing cowpea aphid-borne mosaic virus (camv). using bio ...19938445363
capsid protein properties of cowpea aphid-borne mosaic virus and blackeye cowpea mosaic virus confirm the existence of two major subgroups of aphid-transmitted, legume-infecting potyviruses.a study of the capsid proteins of different legume-infecting potyviruses using specific monoclonal antibodies on immunoblots of crude extracts from infected plants revealed that cowpea aphid-borne mosaic virus (camv) and blackeye cowpea mosaic virus (bicmv) have coat protein m(r) values of 32k and 35k, respectively. immunoblot comparisons of bicmv, peanut stripe mosaic virus (pstv), bean common mosaic virus (bcmv) and azuki bean mosaic virus (azmv) revealed equal reactivity of their 35k coat pro ...19947996147
serological and biological relationships among viruses in the bean common mosaic virus subgroup.bean common mosaic virus (bcmv), blackeye cowpea mosaic virus (b1cmv), cowpea aphid-borne mosaic virus (cabmv), azuki bean mosaic virus (azmv), and peanut stripe virus (pstv) are five species of the genus potyvirus, family potyviridae which are seed-transmitted in beans or cowpeas. eighteen isolates of bcmv, five isolates of b1cmv, four isolates of cabmv, and one isolate each of azmv, and pstv were compared serologically using a panel of 13 monoclonal antibodies (mabs) raised against bcmv, b1cmv ...19921450766
a proposal for a bean common mosaic subgroup of order to elucidate the taxonomic positions of bean common mosaic virus (bcmv) and blackeye cowpea mosaic virus (b1cmv), several strains of these viruses were compared on the basis of host ranges, antigenic properties established with antisera to virions and to n-terminal peptide domains of their coat proteins, and high performance liquid chromatographic peptide profiles. the comparison includes three strains of bcmv, viz. nl1, nl3 and ny15, four strains of b1cmv, viz. fla, ind, nr, and w, and ...19921450765
identification of plant protein kinases in response to abiotic and biotic stresses using supersage.plants are sessile organisms subjected to many environmental adversities. for their survival they must sense and respond to biotic and abiotic stresses efficiently. during this process, protein kinases are essential in the perception of environmental stimuli, triggering signaling cascades. kinases are among the largest and most important gene families for biotechnological purposes, bringing many challenges to the bioinformaticians due to the combination of conserved domains besides diversified r ...201121827428
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