coat protein properties suggest that azuki bean mosaic virus, blackeye cowpea mosaic virus, peanut stripe virus, and three isolates from soybean are all strains of the same potyvirus.the interrelationship of a number of potyviruses infecting legumes has been investigated by comparing molecular properties of their coat proteins. comparison of the coat proteins by the techniques of amino acid analysis and page was inadequate to distinguish strains from distinct potyviruses. however, high-performance liquid chromatographic peptide profiles of tryptic digests of coat proteins of these legume-infecting potyviruses enabled such assignments to be made. these data indicate that amin ...19921500273
[use of immunoenzyme analysis on nitrocellulose membranes for assessing different methods of purifying the yellow mosaic virus of beans].the comparative study of different methods for the purification of bean yellow mosaic virus isolated from lupine has been made. to evaluate the method of purification, electron-microscopic, spectrophotometric and elisa techniques have been used. the possibility of using elisa on nitrocellulose filters with the utilization of a peroxidase-antiperoxidase complex, manufactured in the ussr, for the determination of viral carriership in plants has been shown.19883284252
particle size of the severe strain of bean yellow mosaic virus. electron microscope investigations. 19674166072
intracellular characterization of bean yellow mosaic virus-induced inclusions by differential enzyme digestion.the composition of crystalline inclusions and dense bands in cells of broad bean leaves infected with bean yellow mosaic virus was studied by differential enzymatic digestion. frozen thick sections were prefixed in glutaraldehyde and exposed to proteinases and nucleases, after which ultrathin sections were prepared for electron microscopy. examination revealed that the crystals were completely digested by pepsin in 30 min, whereas the dense bands remained intact for the first 20 min, and could n ...19684874490
correlation of transmission of bean yellow mosaic virus with salivation activity of acyrthosiphon pisum (homoptera: aphididae). 19695374177
construction of a representative cdna library from mrna isolated from mouse oocytes.a representative cdna library has been constructed from the small quantities of poly(a)+ rna present in unfertilised mouse oocytes. the construction of this library has been achieved by use of cow pea mosaic virus rna as a carrier during isolation of polyadenylated message and during subsequent cloning procedures. this approach may be applicable to any system in which amounts of mrna are limiting.19863753684
molecular cloning, sequencing and expression in escherichia coli of the bean yellow mosaic virus coat protein gene.the sequence of 1015 nucleotides from the 3' poly(a) tract of the potyvirus bean yellow mosaic virus (bymv) rna has been determined from two cdna clones. this sequence contained a single long open reading frame (orf) starting upstream of the cloned region. the orf was expressed as a fusion protein in escherichia coli, and the product was detected by antibodies specific for the coat protein of bymv. the predicted length of the coat protein gene was 822 nucleotides, corresponding to a 273 amino ac ...19892671258
monoclonal antibodies produced to bean yellow mosaic virus, clover yellow vein virus, and pea mosaic virus which cross-react among the three viruses.monoclonal antibodies prepared against individual potyviruses that infect forage legumes cross-reacted among the viruses. the reaction occurs between capsid subunits and presumably involves epitopes located in the trypsin-resistant core of the coat protein.19892480762
physical mapping and molecular cloning of mung bean yellow mosaic virus dna.viral single-stranded dna of mung bean yellow mosaic virus (mymv) was converted to the double-stranded state in vitro, and physical mapping was carried out. the genome of mymv was found to consist of two major components (designated as dna 1 and dna 2). in addition, some minor components were detected. molecular cloning of the major components was carried out, using in vitro double-stranded dna and replicative intermediate dnas. dna 1 is about 2.72 and dna 2 about 2.67 kilobase pairs. no similar ...19902159956
identification of potyvirus isolates from faba bean (vicia faba l.), and the relationships between bean yellow mosaic virus and clover yellow vein virus.clover yellow vein virus (cyvv) isolate h and the related potyvirus isolates e178, e197, and e242 could be distinguished from bean yellow mosaic (bymv) isolates by their wider host range among non-legume test plant species, the peculiar enlargement of the nucleolus in infected plants, and the larger size of their coat protein as evidenced by slower migration in sds-page. serologically, they are qualitatively indistinguishable in electro-blot immunoassay (ebia) also with antibodies specific to th ...19912048976
selection of polyclonal antibodies to the n terminus of bean yellow mosaic potyvirus coat protein by induction of tolerance with monoclonal antibody.polyclonal antisera to potyviruses contain virus-specific as well as cross-reacting antibodies. the virus-specific antibodies are directed to the surface-located, n-terminal region of the coat protein, whereas cross-reacting antibodies are produced against multiple epitopes within the core region of the coat protein (minus n and c termini), which displays extensive sequence homology among distinct potyviruses. in the present study, immunological tolerance was induced in mice against the cross-re ...19911955491
relatedness of the nucleotide sequence of the 3'-terminal region of clover yellow vein potyvirus rna to bean yellow mosaic potyvirus rna.the sequence of the 3'-terminal 1,357 nucleotides of clover yellow vein potyvirus rna was determined. a coat protein gene was identified and its predicted amino acid sequence deduced. it had 273 residues with a molecular weight of 31,019. the amino acid and nucleotide sequences of the virus were compared with those of five other potyviruses. the homology between these viruses indicated that clover yellow vein virus was a distinct virus, but had a closer affinity to bean yellow mosaic virus than ...19911864719
bean yellow mosaic, clover yellow vein, and pea mosaic are distinct potyviruses: evidence from coat protein gene sequences and molecular hybridization involving the 3' non-coding regions.the sequences of the 3' 1019 nucleotides of the genome of an atypical strain of bean yellow mosaic virus (bymv-s) and of the 3' 1018 nucleotides of the clover yellow vein virus (cyvv-b) genome have been determined. these sequences contain the complete coding region of the viral coat protein followed by a 3' non-coding region of 173 and 178 nucleotides for bymv-s and cyvv-b, respectively. when the deduced amino acid sequences of the coat protein coding regions were compared, a sequence identity o ...19921731696
comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies.a panel of monoclonal antibodies (mabs) generated against an admixture of 12 potyvirus isolates was used to compare and differentiate diverse potyviruses. both native and denatured virions of strains of bean yellow mosaic (bymv), potato virus y, tobacco etch, pea seed-borne mosaic, iris severe mosaic, iris mild mosaic and asparagus virus-1 potyviruses were used as immunogen and as antigen for screening of the hybridoma cell lines. thirty cell lines secreting potyvirus-specific antibodies reactiv ...19911703561
cdna cloning and sequencing of the bean yellow mosaic virus nuclear inclusion protein genes. 19921600157
bean yellow mosaic virus subgroup; search for the group specific sequences in the 3' terminal region of the order to examine relationships among viruses of the bean yellow mosaic subgroup of the potyvirus genus, several isolates of bean yellow mosaic virus (bymv) and clover yellow vein virus (c1yvv) were compared by amino acid sequence of the coat protein and nucleotide sequence of the 3' terminal non-coding region. the sequence comparisons showed that bymv and c1yvv were distinct viruses but had close affinity to each other (85-95% homology among isolates of a virus but 70-77% homology between vir ...19921450764
potyviruses, chaos or order?at first potyviruses were easily distinguished by biological and serological properties because only a few were known and information on their host ranges was limited. the first evidence of serological cross reaction between two of these viruses was reported in 1951 and was further corroborated for three obviously distinct members of the group in 1960. in 1968 attention was drawn to the fact that some legume and non-legume potyviruses have much wider host ranges than previously known and that wi ...19921450755
potyviruses, monoclonal antibodies, and antigenic sites.virus-specific and cross-reactive monoclonal antibodies have been produced to at least 19 different aphid-transmitted potyviruses. this report summarizes the development of these monoclonal antibodies as well as presents information on the delineation of the virus-specific and group-common epitopes defined by these monoclonal antibodies. virus-specific and group-common antigenic determinants were mapped by a variety of techniques, including analysis of antigen: antibody reactivity patterns, dete ...19921280511
nodule infection by bean yellow mosaic virus in phaseolus vulgaris.infection of root nodules of beans, phaseolus vulgaris l., by bean yellow mosaic virus (bymv) and the effect of the disease on the specific activity of the nodule are reported. infectivity and serological microprecipitin assays with two sources of bymv antiserum demonstrated that nodules from bean plants whose leaves had been inoculated with bymv contain bymv antigen. the disease reduced the fresh weights of tops, roots, and root nodules and induced premature nodule decay and/or nodule drop. the ...1978736539
changes in chlorophyll and primary productivity of pea (pisum sativum l.) leaves as influenced by arhar (pigeon pea) mosaic virus infection. 1979554306
serological differences between some isolates of bean yellow mosaic virus.the degree of serological relatedness of three isolates of bean yellow mosaic virus (bymv) was studied. one common antigenic group (m) was demonstrated in all 3 isolates. in addition, another antigenic group (a) was demonstrated in the isolate fvm1. this group was absent from the isolates tpm3 and tpm11, in which the antigenic group n was found. antisera to the 3 isolates contained the corresponding antibody groups. the antibody group (m) shared by all 3 bymv isolates was present in the fvm1 ant ...19751992
an absolute requirement for the 5' cap structure for mrna translation in sea urchin eggs.translation of a variety of rnas was studied in a cell-free translation system derived from sea urchin eggs. while rnas such as globin or tobacco mosaic virus are efficiently translated, viral rnas which do not contain the 5' cap structure, such as cow pea mosaic virus (cpmv) and poliovirus, are not translated. mixing experiments with reticulocyte lysates indicated that the lack of translation of uncapped viral rnas is not due to the presence of a potent inhibitor or the absence of an activating ...19836653555
a mosaic disease of senna hirsuta induced by a potyvirus in nigeria.a virus inducing mosaic and severe leaf malformation, isolated from senna hirsuta in nigeria, was studied. the virus had a rather narrow host range, infecting a few species in caesalpinaceae, chenopodiaceae and fabaceae families. the virus was widespread in southern nigeria with prevalence ranging from 74% to 86.4% in some locations. it was transmitted mechanically and in a non-persistent manner by myzuspersicae, aphis craccivora and a. spiraecola. there was no evidence of transmission by seeds. ...200111719985
intraspecies variability in transmission efficiency of stylet-borne viruses by the pea aphid (acyrthosiphon pisum).the efficiency of bean yellow mosaic virus (bymv; potyvirus group) tranasmission from pea to pea plants by 36 clones of the pea aphid (acyrthosiphon pisum) was studied. the efficiency of the clones varied; it was not related to the aphid colour form (green, red or yellow) or the origin of the clone (host plant, geographic area). with alfalfa mosaic virus, the virus-vector relationships concerning transmission efficiency were, in general, similar to those found with bymv. the behaviour of the clo ...19806108064
fine structure of inclusions and organelles in vicia faba infected with bean yellow mosaic virus. 19665932839
evaluation of plant susceptibility to infection with bean yellow mosaic virus by aphid transmission. 19665905293
[some relations between the common pea mosaic virus and the peach tree aphis myzus persicae sulz]. 19655856645
vector-virus relationship of two less known aphid vectors of pea mosaic virus. 19675626183
[properties of bean yellow mosaic virus isolates, obtained from clover]. 19715559330
effects of temperature on plant susceptibility to infection with bean yellow mosaic virus by aphid transmission. 196414204703
occurrence and sequences of lily mottle virus and lily symptomless virus in plants grown from imported bulbs in zhejiang province, china.degenerate primers were used to amplify virus sequences from imported lilies in zhejiang province, china. two viruses, lily mottle virus (lmov, genus potyvirus) and lily symptomless virus (lsv, genus carlavirus) were detected, purified and completely sequenced from a mixed infection in a plant raised from bulbs imported from the netherlands. the sequence of lmov was 9644 nt long and encoded a polyprotein of 3095 amino acids with a calculated m(r) of 351.0 kda that had only 45.1-54.4% identity to ...200314648295
the complete nucleotide sequence of isolate bymv-gdd of bean yellow mosaic virus, and comparison to other potyviruses.the complete nucleotide sequence of bean yellow mosaic virus (bymv) gladiolus isolate gdd was determined and compared to broad bean isolates bymv-mb4 and bymv-s. the bymv-gdd genome (9528 nt) was more similar to bymv-mb4 (9532 nt) than to bymv-s (9547 nt), which has "atypical" symptom expression and host range. the greatest variability occurred in the 5' untranslated region, p1 protein, and nia-vpg protein, the n-terminal two thirds of hc-pro, and the c-terminal one third of p3. each of these re ...200314648299
[the effect of salivary gland (obtained from myzus persicae sulzer) extract on broad bean epidermal cells infected with bean yellow mosaic virus (author's transl)]. 19744478240
single-stranded dna in mung bean yellow mosaic virus. 19854069018
immunochromatographic purification of bean yellow mosaic virus.the method of immunoadsorptional purification of bean yellow mosaic virus has been worked out. immunosorbents were obtained by coupling the antibody (igg) fraction isolated from anti-bymv and anti-pea leaf protein antisera with cnbr-activated 1% agarose beads. conditions for preparation of immunosorbents, for bymv adsorption and elution as well as the method of plant protein separation from bymv were pointed out. the purity of bymv was checked by double immunodiffusion as well as by sds-acrylami ...19817025790
effective resistance to potyvirus infection conferred by expression of antisense rna in transgenic plants.approximately 660 nt including the carboxy-terminal portion of the bean yellow mosaic potyvirus (bymv) coat protein gene, complete 3' noncoding sequence and a short poly(a) tract were introduced to produce antisense rna in transgenic nicotiana benthamiana. original (r0) transformants were selfed, and homozygous second generation (r2) populations challenged with infected sap, purified virus, or viral rna. one transgenic line of 10 examined was extremely resistant to infection by mechanical inocul ...19957579613
effect of nitrogen fixation, nitrogen fertilization and viral infection on yield, tannin and protein contents and in vitro protein digestibility of faba bean.a field investigation of two faba bean cultivars (cv.), agabat and silaim, showed that bean yellow mosaic virus (bymv) infection reduced (p < or = 0.001) yield (kg/ha), protein content and in vitro protein digestibility (ivpd) but increased (p < or = 0.05) tannin content (mg/100 ml). nitrogen fertilization with viral infection significantly reduced yield and ivpd for cv. silaim and increased (p < or = 0.05) protein and tannin contents. nitrogen fertilization alone was found to increase (p < or = ...19957659703
the complete nucleotide sequences of the coat protein cistron and the 3' non-coding region of a newly-identified potyvirus infecting sweetpotato, as compared to those of sweetpotato feathery mottle virus.complementary dna representing 728 nucleotides of the 3' end of the genomic rna of sweetpotato virus g (spv-g) a newly-identified potyvirus infecting sweetpotato, was cloned and sequenced. this sequence was combined with that previously determined for the 5' terminal part of the coat protein cistron of the virus. the whole sequence contained a single open reading frame (orf) of 1065 nucleotide, with the capacity to encode a coat protein of 355 amino acids, significantly larger than that of other ...19947832639
resistance of green peas to legume viruses.a total of 16 green pea cultivars (pisum sativum l.) were screened for resistance to bean yellow mosaic virus (bymv), pea enation mosaic virus (pemv), and alfalfa mosaic virus (amv). cvs. avola, midget, wawerplus, wawerex, bunny and banff were found immune to bymv infection. cvs. marx and pion exhibited a high level (above 95%) of resistance to bymv, but they were highly susceptible to pemv and amv. it is recommended to use bymv-immune or highly bymv-resistant cultivars for further breeding. the ...19947976868
nucleotide sequence of the 3'-terminal region of the genome confirms that pea mosaic virus is a strain of bean yellow mosaic potyvirus.the 1,035 nucleotides at the 3'end of the i strain of pea mosaic potyvirus (pmv-i) genomic rna, encoding the coat protein, have been cloned and sequenced. a comparison of the derived coat protein sequence with those of the bean yellow mosaic virus (bymv) strains, cs, s, d and gdd, indicates that pmv-i is a strain of bymv. sequence comparisons and hybridisation studies using the 3'-noncoding region support this classification. the nucleotide and protein sequence data also suggest that pmv-i and b ...19948031241
effects of introgression and recombination on haplotype structure and linkage disequilibrium surrounding a locus encoding bymovirus resistance in barley.we present a detailed analysis of linkage disequilibrium (ld) in the physical and genetic context of the barley gene hv-eif4e, which confers resistance to the barley yellow mosaic virus (bymv) complex. eighty-three snps distributed over 132 kb of hv-eif4e and six additional fragments genetically mapped to its flanking region were used to derive haplotypes from 131 accessions. three haplogroups were recognized, discriminating between the alleles rym4 and rym5, which each encode for a spectrum of ...200717151251
evaluation of linked pcr-transcription amplification procedure for bean yellow mosaic virus detection in gladioli.bean yellow mosaic virus (bymv) concentration in in-vitro cultured gladiolus cormlets was low and impossible to detect by the commonly used diagnostic methods. the polymerase chain reaction (pcr) detected viral rna in most infected cormlets but not in all. additional amplification of the pcr products by transcription, using t7 rna polymerase (pcr/t), resulted in virus detection in cases which otherwise went undetected. pcr products having a single polymerase promoter at one end served as a bette ...19948051229
resistance to erysiphe polygoni of red clover infected with bean yellow mosaic virus.the development of erysiphe polygoni on leaves of red clover was retarded by poor infection with bean yellow mosaic virus. conidia germinated on leaves infected with the virus but disintegrated following necrosis of the leaf tissue in the immediate area of the germinating conidia.196417832405
a method combining immunocapture and pcr amplification in a microtiter plate for the detection of plant viruses and subviral pathogens.a method for the detection of rna viral and subviral plant pathogens was developed that combines pathogen partial purification by solid-phase adsorbed antibodies, reverse transcriptional-polymerase chain reaction (rt-pcr) and quantitation of the amplified products by fluorescence. the reverse transcription of the rna is performed directly on the retained material without any previous thermal or chemical disruption of the virus particles. the whole procedure can be carried out in a microtiter pla ...19938113346
a surprising effect of extraction conditions on the mobility of some plant virus coat proteins in sds-page.the coat protein from purified particles of pea seedborne mosaic potyvirus (psbmv) moves in sds-page with an apparent molecular weight (m(r)) of 36 kda. however, extracts of psbmv infected plants prepared with sds or urea contain psbmv immunoreactive proteins with apparent m(r) 39 kda as well as 36 kda. the low mobility form may be generated from the apparent m(r) 36 kda form by incubating purified psbmv particles with healthy plant sap in the presence of denaturing agents. a similar effect is o ...19948188819
the nucleotide sequence and genome structure of mung bean yellow mosaic geminivirus.complete nucleotide sequences of the infectious cloned dna components (dna 1 and dna 2) of mung bean yellow mosaic virus (mymv) were determined. mymv dna 1 and dna 2 consists of 2,723 and 2,675 nucleotides respectively. dna 1 and dna 2 have little sequence similarity except for a region of approximately 200 bases which is almost identical in the two molecules. analysis of open reading frames revealed nine potential coding regions for proteins of mol. wt. > 10,000, six in dna 1 and three in dna 2 ...19938231962
nucleotide and deduced amino acid sequence of the 3' end of the bymv-mi genome.we have cloned and sequenced cdna transcribed from the 3' 1,239 nucleotides of the genomic rna of a western australian isolate (mi) of bean yellow mosaic potyvirus (bymv). this sequence contains 246 nucleotides of the nib (replicase) gene and 819 nucleotides representing the entire coding region of the viral coat protein gene, followed by a 3' non-coding region of 174 nucleotides. the coding region of the coat protein gene is identical in length (273 amino acids) to that already reported for oth ...19958572947
the complete nucleotide sequence of bean yellow mosaic potyvirus rna.the complete nucleotide sequence of an australian strain of bean yellow mosaic virus (bymv-s) has been determined from cloned viral cdnas. the bymv-s genome is 9 547 nucleotides in length excluding a poly(a) tail. computer analysis of the sequence revealed a single long open reading frame (orf) of 9168 nucleotides, commencing at position 206 and terminating with uag at position 9374-6. the orf potentially encodes a polyprotein of 3056 amino acids with a deduced mr of 347 409. the 5' and 3' untra ...19968774684
encapsidation of potyviral rna in various forms of transgene coat protein is not correlated with resistance in transgenic plants.transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (cp) were inoculated with various potyviruses. antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from < 1% to as much as 25% transgenic cp. different levels of transcapsidation may reflect the extent of compatibility between transgene cp and the viral cp.19979353947
preparation of diagnostic monoclonal antibodies against two potyviruses.diagnostic monoclonal and polyclonal antibodies against bean yellow mosaic virus (bymv) and plum pox virus (ppv) were prepared, characterized and used for detection of these viruses in infected plants by enzyme-linked immunosorbent assay (elisa), immunoblot analysis and tissue print immunoblot assay (tpiba).199910749372
characterization of the immune response to canine parvovirus induced by vaccination with chimaeric plant viruses.nih mice were vaccinated subcutaneously or intranasally with chimaeric cow pea mosaic virus (cpmv) constructs expressing a 17-mer peptide sequence from canine parvovirus (cpv) as monomers or dimers on the small or large protein surface subunits. responses to the chimaeric virus particles (cvps) were compared with those of mice immunized with the native virus or with parvovirus peptide conjugated to keyhole limpet haemocyanin (klh). the characteristics of the immune response to vaccination were e ...200212034099
a non-aphid-transmissible isolate of bean yellow mosaic potyvirus has an altered nag motif in its coat isolate of bean yellow mosaic virus (bymv) not transmitted by aphids (nat) was compared with the aphid-transmissible isolate (mi) from which it was derived. for each isolate, the sequence of the coat protein and parts of the helper component was determined. a single nucleotide substitution caused a nag to nas alteration in the coat protein of the non aphid-transmissible isolate. loss of aphid transmissibility in isolate bymv(mi)-nat was most likely caused by this mutation within the nag motif ...200212209319
evidence for diversifying selection in potato virus y and in the coat protein of other potyviruses.the modes of evolution of the proteins of potato virus y were investigated with a maximum-likelihood method based on estimation of the ratio between non-synonymous and synonymous substitution rates. evidence for diversifying selection was obtained for the 6k2 protein (one amino acid position) and coat protein (24 amino acid positions). amino acid sites in the coat proteins of other potyviruses (bean yellow mosaic virus, yam mosaic virus) were also found to be under diversifying selection. most o ...200212237440
the 3' terminal region is strictly required for clover yellow vein virus genome identify the cis-element in the 3' terminal region of infectious cdna required for replication of clover yellow vein virus (clyvv), a series of mutants with duplications or deletions of the 3' terminal non-coding region (3'-ncr) of the genome that did not affect the orfs in the genome was constructed. these were tested for infectivity, and the 3' terminal regions of their progeny rnas were sequenced. deletion mutants that lacked portions of the 3'-ncr were not infectious. various mutants with ...200312664298
fabrication of assembled virus nanostructures on templates of chemoselective linkers formed by scanning probe nanolithography.we have developed a multistep route to the fabrication of virus assembled nanostructures with chemoselective protein-to-surface linkers synthesized by an efficient solid-phase method. these linkers were used to create patterns of 30-to-50-nm-width-lines by scanning probe nanolithography. genetically modified cow pea mosaic virus with unique cysteine residues at specific locations on their capsomers were assembled through covalent linkage on these patterns. the morphology of the assembled structu ...200312783520
[pea mosaic virus in czechoslovakia]. 195513261210
the dna-a component of a plant geminivirus (indian mung bean yellow mosaic virus) replicates in budding yeast cells.understanding the biochemistry of dna replication of the plant dna viruses is important for the development of antiviral strategies. since dna replication is little studied in plants, a genetically tractable, easily culturable, eukaryotic model system is required to pursue such studies in a facile manner. here we report the development of a yeast model system that supports dna replication of a chosen geminivirus strain, indian mung bean yellow mosaic virus. the replication of plasmid dna in the ...200414963136
using epidemiological information to develop effective integrated virus disease management strategies.virus diseases cause serious losses in yield and quality of cultivated plants worldwide. these losses and the resulting financial damage can be limited by controlling epidemics using measures that minimise virus infection sources or suppress virus spread. for each combination of virus, cultivated plant and production system, there is an 'economic threshold' above which the financial damage is sufficient to justify using such measures. however, individual measures used alone may bring only small ...200415036832
association of cucumovirus and potyvirus with betelvine (piper betle l.) as evidenced by elisa and attempt was made to detect various viruses of piper betle grown at mahoba and banthara in india. dac-elisa and rt-pcr tests were performed in leaf sap samples of betelvine for detection of a cucumovirus (cucumber mosaic virus) and potyvirus (bean yellow mosaic virus) using specific antibodies and universal primers of respective viruses. dac-elisa could detect only cmv. however, rt-pcr detected both cucumovirus and potyvirus infection in betelvine samples. association of cmv with betelvine was ...200315267161
pcna interacts with indian mung bean yellow mosaic virus rep and downregulates rep activity.proliferative cell nuclear antigen (pcna), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. the biochemical role of pcna in rolling circle replication (rcr) of geminivirus dna has not been explored in detail. the initiation of rcr of the bipartite genome of a geminivirus, indian mung bean yellow mosaic virus (imymv), is mainly controlled by viral protein rep (or al1 or ac1). the role ...200415479830
transgenic gladiolus plants transformed with the bean yellow mosaic virus coat-protein gene in either sense or antisense orientation.transgenic gladiolus plants transformed with the bean yellow mosaic virus (bymv) coat-protein (cp) gene in either sense or antisense (as) orientation were developed using biolistics. four of the plants were confirmed to carry the cp gene in the sense orientation of the gene and seven plants in the as orientation. two of the cp plant lines and all of the as lines showed dna rearrangements of the transgene in addition to an intact copy of the transgene. the copy number ranged from one to nine. of ...200515480682
properties of a virus isolated from vernonia amygdalina del. in lagos, nigeria.a previously uncharacterized virus tentatively named vernonia green vein-banding virus (vgvbv) was isolated from vernonia amygdalina del. ("bitterleaf") from lagos, nigeria. the virus was mechanically transmissible but had a narrow host range restricted to nicotiana benthamiana, chenopodium quinoa and c. amaranticolor. it was also transmissible in a non-persistent manner by myzus persicae. the virus was purified from n. benthamiana and about 750 nm long flexuous rod-shaped particles were observe ...200415745049
attributes of bean yellow mosaic potyvirus transmission from clover to snap beans by four species of aphids (homoptera: aphididae).after characterization of the natural spread of necrosis-inducing bean yellow mosaic potyvirus (family potyviridae, genus potyvirus, bymv(n)), nonpersistently transmitted from clover, trifolium repens l., to an adjacent field of snap bean, phaseolus vulgaris l., in western oregon, we established a study site enabling us to investigate the virus reservoir, to observe en masse transmission of bymv(n) to bean plants, and to identify aphid species associated with virus spread. colonies of myzus pers ...200516539099
dielectrophoretic investigation of plant virus particles: cow pea mosaic virus and tobacco mosaic virus.this paper reports experimental results on the dielectrophoretic (dep) behaviour on two nonenveloped plant viruses of different geometrical shapes, namely cow pea mosaic virus (cpmv) and tobacco mosaic virus (tmv). the dep properties of carboxy-modified latex beads of the same size are also reported. the dep properties of single particles were obtained from measurement of the frequency at which the dep force on a particle goes to zero (the crossover frequency). the dep behaviour of particle ense ...200617054097
occurrence of viruses infecting pea in iran.a survey was conducted to determine the incidence of alfalfa mosaic virus (amv), bean yellow mosaic virus (bymv), broad bean wilt virus-1 (bbwv), pea leafroll virus (plrv), pea enation mosaic virus (pemv), pea seed borne mosaic virus (psbmv), potato virus x(pvx), tomato mosaic virus (tomv), tomato spotted wilt virus (tswv) on pea (pisum sativum) in iran. a total of 1276 random and 684 symptomatic pea samples were collected during the spring and summer of 2002-2004 in tehran province of iran, whe ...200617390891
the same allele of translation initiation factor 4e mediates resistance against two potyvirus spp. in pisum sativum.pathogenicity of two sequenced isolates of bean yellow mosaic virus (bymv) was established on genotypes of pisum sativum l. reported to carry resistance genes to bymv and other potyviruses. resistance to the white lupin strain of bymv (bymv-w) is inherited as a recessive gene named wlv that maps to linkage group vi together with other potyvirus resistances. one of these, sbm1, confers resistance to strains of pea seedborne mosaic virus and previously has been identified as a mutant allele of the ...200717849710
protective action of salicylic acid against bean yellow mosaic virus infection in vicia faba this study, morphological, ultrastructural and physiological modifications of faba bean (vicia faba cv giza 461) leaves in response to bean yellow mosaic virus (bymv) infection and salicylic acid (sa) treatments were examined. under bymv stress, leaves showed symptoms including severe mosaic, mottling, crinkling, size reduction and deformations. three weeks after virus inoculation, photosynthetic rate, pigment contents and transpiration rate were significantly reduced in response to bymv infe ...200817920158
construction of infectious cdna clones derived from the potyviruses clover yellow vein virus and bean yellow mosaic virus.infectious cdna clones are now indispensible tools for the genetic analysis of viral factors involved in viral virulence and host resistance. in addition, infectious cdna-derived virus vectors that express foreign genes in infected plants enable the production of useful proteins at low cost and can confer novel crop traits. we constructed infectious cdna clones derived from two potyviruses, clover yellow vein virus and bean yellow mosaic virus, which infect legume plants and cause disease. here, ...201525287506
a comparison of the nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses.3h-labelled complementary dna (cdna) was reverse transcribed from the rna of three biologically distinct isolates of bean yellow mosaic virus (bymv-g, q, and s) by the random primer method of taylor et al. [biochim. biophys. acta 442, 324-330 (1976)]. excess virus rna was hybridized with each of the cdnas and percentage hybrid formation was assayed using the single-strand-specific nuclease s1, the r0t (1/2) values obtained for the homologous hybridization reactions (1.2 x 10(-2) mol sec liter(-1 ...198118635050
towards a system for the identification and classification of potyviruses. ii. virus particle length, symptomatology, and cytopathology of six distinct viruses.the mean particle lengths of lettuce mosaic virus (lmv), bean common mosaic virus (bcmv), passionfruit woodiness virus (pwv), potato virus y (pvy), sugarcane mosaic virus (scmv), and one strain of bean yellow mosaic virus (bymv) were all shown to be close to 750 nm. however, the length of two other serologically closely related strains of bymv, variants of pea mosaic virus referred to as pmv and spmv, had significantly longer particles, about 792 and 842 nm. each of the distinct viruses had read ...198118635068
ultrastructure of mixed plant virus infection: bean yellow mosaic virus with cowpea severe mosaic virus or cowpea mosaic virus in bean.ultrastructural responses of bean leaf cells simultaneously infected with two morphologically distinct rna viruses, cowpea mosaic virus (cpmv) and bean yellow mosaic virus (bymv), or cowpea severe mosaic virus (csmv) and bymv, were studied in situ. the major effects on cells infected with two viruses included: (1) association of virus group-specific cytoplasmic inclusions characteristic of each virus; (2) close association of virions into specifically arranged aggregates in which cpmv or csmv ic ...198318638866
analysis of the spatial distribution of identical and two distinct virus populations differently labeled with cyan and yellow fluorescent proteins in coinfected plants.abstract apple latent spherical virus (alsv) expressing yellow and cyan fluorescent proteins (alsv-yfp and alsv-cfp) was used to investigate the distribution of identical virus populations in coinfected plants. in chenopodium quinoa plants inoculated with a mixture of alsv-yfp and alsv-cfp, fluorescence from yfp and cfp was always distributed separately in both inoculated and upper uninoculated leaves. inoculation of each alsv-yfp and alsv-cfp to different leaves of a c. quinoa plant resulted in ...200718943677
development and evaluation of a complementation-dependent gene delivery system based on cucumber mosaic engineer cucumber mosaic virus (cmv-ix) into a gene vector, genome component rna 3 of the virus was modified and split into two sub-components, rna 3a and rna 3b. in rna 3a, the open reading frame of the movement protein (mp) was replaced by a reporter gene encoding the green fluorescent protein (gfp), to monitor virus replication and movement. in rna 3b, the coat protein (cp) gene was eliminated and a multiple cloning site (mcs) was created for foreign gene insertion. each sub-component alon ...200011205118
preventive and curative effects of apple latent spherical virus vectors harboring part of the target virus genome against potyvirus and cucumovirus latent spherical virus (alsv)-based vectors experimentally infect a broad range of plant species without causing symptoms and can effectively induce stable virus-induced gene silencing in plants. here, we show that pre-infection of alsv vectors harboring part of a target viral genome (we called alsv vector vaccines here) inhibits the multiplication and spread of the corresponding challenge viruses [bean yellow mosaic virus, zucchini yellow mosaic virus (zymv), and cucumber mosaic virus (cm ...201324074595
antibodies against a truncated staphylococcus aureus fibronectin-binding protein protect against dissemination of infection in the rat.staphylococcus aureus bacteraemia (sab) originating from local infections can lead to severe secondary infections such as endocarditis. the protective effect of antibodies against secondary infections was studied in a rat model, where a local joint infection leads to bacteraemia and endocarditis on damaged aortic valves. in this study, immunizations with a truncated d2-domain of the s. aureus fibronectin-binding protein displayed on a cow-pea mosaic virus (cpmv-d) carrier induced protection agai ...200111348701
an epidemiological model for externally sourced vector-borne viruses applied to bean yellow mosaic virus in lupin crops in a mediterranean-type environment.a hybrid mechanistic/statistical model was developed to predict vector activity and epidemics of vector-borne viruses spreading from external virus sources to an adjacent crop. the pathosystem tested was bean yellow mosaic virus (bymv) spreading from annually self-regenerating, legume-based pastures to adjacent crops of narrow-leafed lupin (lupinus angustifolius) in the winter-spring growing season in a region with a mediterranean-type environment where the virus persists over summer within dorm ...200819000002
activation of the salicylic acid signaling pathway enhances clover yellow vein virus virulence in susceptible pea cultivars.the wild-type strain (cl-wt) of clover yellow vein virus (clyvv) systemically induces cell death in pea cv. plant introduction (pi) 118501 but not in pi 226564. a single incompletely dominant gene, cyn1, controls systemic cell death in pi 118501. here, we show that activation of the salicylic acid (sa) signaling pathway enhances clyvv virulence in susceptible pea cultivars. the kinetics of virus accumulation was not significantly different between pi 118501 (cyn1) and pi 226564 (cyn1); however, ...200919132869
rhizobium leguminosarum inoculation decreases damage to faba bean (vicia faba) caused by broad bean mottle bromovirus and bean yellow mosaic potyvirus.faba bean (vicia faba) plants were inoculated with rhizobia and then their sap was infected with broad bean mottle bromovirus (bbmv) or bean yellow mosaic potyvirus (bymv) in a field experiment. both viral infections significantly decreased shoot and root dry weight, number of nodules, nodule dry weight, numbers of flowers and pods/plant, total plant n, grain yield and n2 fixation. however, inoculation withrhizobium leguminosarum significantly increased all these parameters, both in healthy and ...199524414508
do plant viruses facilitate their aphid vectors by inducing symptoms that alter behavior and performance?aphids can respond both positively and negatively to virus-induced modifications of the shared host plant. it can be speculated that viruses dependent on aphids for their transmission might evolve to induce changes in the host plant that attract aphids and improve their performance, subsequently enhancing the success of the pathogen itself. we studied how pea aphids [acyrthosiphon pisum (harris)] responded to infection of tic beans (vicia faba l.) by three viruses with varying degrees of depende ...200819161702
hybrid assembly of cpmv viruses and surface characteristics of different mutants.there is a trend toward viral-based hybrid systems to furnish viral nanoparticles with enhanced features, for function beyond a delivery vehicle. such hybrids have included nanogold for microwave release, quantum dots and fluorescent moieties, to provide simultaneous imaging capabilities, and iron oxide particles for image enhancement in mri. other systems are the subject of ongoing and vigorous research. nanogold surface decoration of cow pea mosaic virus (cpmv) to form ng-cpmv hybrids were exp ...200919198570
cross-protection against bean yellow mosaic virus (bymv) and clover yellow vein virus by attenuated bymv isolate m11.attenuated isolate m11 of bean yellow mosaic virus (bymv), obtained after exposing bymv-infected plants to low temperature, and its efficacy in cross-protecting against infection by bymv isolates from gladiolus, broad bean (vicia faba) and white clover (trifolium repens) was assessed with western blotting and reverse transcription-polymerase chain reaction. the level of cross-protection varied depending on the challenge virus isolates. cross-protection was complete against bymv isolates from gla ...200919203277
nodule infection by bean yellow mosaic virus in vicia faba and molecular characterization of it.root nodules infection of different faba bean (vicia faba l.) cultivars by bean yellow mosaic virus (bymv) and the effect of the disease on the specific activity of the nodule are reported. the disease reduced the fresh weights of tops, roots, root nodules and induced premature nodule decay and/or nodule drop. six local cultivars: barekat, iranshahri, saraziri, aljazayeri, shakhbozi and zohre of faba bean were selected and inoculated with bymv under greenhouse conditions. elisa test (das-elisa) ...200819226767
role of recombination in the evolution of host specialization within bean yellow mosaic complete genomes and 64 coat protein gene sequences belonging to bean yellow mosaic virus (bymv) isolates from different continents were examined for evidence of genetic recombination using six different recombination-detection programs. in the seven complete genomes and a single complete genome of the related virus clover yellow vein virus (clyvv), evidence for eight recombination patterns was found by four or more programs, giving firm evidence of their presence, and five additional reco ...200919351247
identification of a new pathotype of bean yellow mosaic virus (bymv) infecting blue passion flower and some evolutionary characteristics of bymv.the modes of molecular evolution of the coat protein (cp) and 3' non-coding region (ncr) were investigated in bean yellow mosaic virus (bymv) isolates, including a new pathotype from blue passion fruit. in phylogenetic analysis, the new pathotype did not cluster with pathotypes or host groups described previously. intraspecific recombinations involving the entire 3'-ncr and a variable portion of the 3'-terminal region of the cp gene were detected between a broad bean isolate and several isolates ...200919763780
variability of bean yellow mosaic virus isolates in the czech republic.three isolates of bean yellow mosaic virus (bymv) from the czech republic originating from gladiolus plants were examined according to their biological and molecular characteristics. partial sequence of coat protein-nuclear inclusion protein b (cp-nib) coding region (768 bp) of these isolates were determined and compared with the corresponding sequences of different bymv isolates obtained from genebank. phylogenetic analysis showed that the czech bymv isolates were distributed across the three g ...200919941392
comparative analysis of elisa, one step rt-pcr and ic-rt-pcr for the detection of bean yellow mosaic virus in overcome the problematic detection of bean yellow mosaic virus (bymv) concentration in gladiolus corms or cormlets, we tested suitability of different diagnostic methods such as elisa, one step rt-pcr and ic-rt-pcr. among these methods, das-elisa and one step rt-pcr method was able to detect virus in leaves and it failed to detect in corms or cormlets whereas ic-rt-pcr method was very sensitive and able to detect virus in both leaves and corms or cormlets of gladiolus plants this is due to du ...200920222572
characterization of a novel toll/interleukin-1 receptor (tir)-tir gene differentially expressed in common bean (phaseolus vulgaris cv. othello) undergoing a defence response to the geminivirus bean dwarf mosaic virus.summary common bean (phaseolus vulgaris l.) cultivar (cv.) othello develops a hypersensitive response-associated vascular resistance to infection by bean dwarf mosaic virus (bdmv), a single-stranded dna virus (genus begomovirus, family geminiviridae). a pcr-based cdna subtraction approach was used to identify genes involved in this resistance response. eighteen clones, potentially involved with bdmv resistance, were identified based upon being up-regulated in bdmv-infected tissues and/or having ...200720507487
silver nanoparticles synthesis mediated by new isolates of bacillus spp., nanoparticle characterization and their activity against bean yellow mosaic virus and human pathogens.extracellular agents produced by newly isolated bacterial strains were able to catalyze the synthesis of silver nanoparticles (agnps). the most effective isolates were identified as bacillus pumilus, b. persicus, and bacillus licheniformis using molecular identification. dls analysis revealed that the agnps synthesized by the above strains were in the size range of 77-92 nm. tem observations showed that the nanoparticles were coated with a capping agent, which was probably involved in nanopartic ...201526029190
seven members of the (1→3)-β-glucanase gene family in barley (hordeum vulgare) are clustered on the long arm of chromosome 3 (3hl).members of the (1→3)-β-glucan glucanohydrolase (ec gene family have been mapped on the barley genome using three doubled haploid populations and seven wheat-barley addition lines. specific probes or polymerase chain reaction (pcr) primers were generated for the seven barley (1→3)-β-glucanase genes for which cdna or genomic clones are currently available. the seven genes are all located on the long arm of chromosome 3 (3hl), and genes encoding isoenzymes gi, gii, giii, giv, gv and gvii ...199624166542
histochemical visualization of ros and antioxidant response to viral infections of vegetable crops grown in azerbaijan.extremes of environmental conditions, such as biotic stresses, strongly affect plant growth and development and may adversely affect photosynthetic process. virus infection is especially problematic in crops, because unlike other diseases, its impact cannot be reduced by phytosanitary treatments. the vegetable crops (solanum lycopеrsicum l, cucurbita melo l., cucumis sativus l., piper longum l., solánum melongéna l., vicia faba l.) showing virus-like symptoms were collected from fields located i ...201424661407
identification and molecular characterization of bean yellow mosaic virus infecting french bean in himachal pradesh.french bean (phaseolus vulgaris l.), is one of the most widely grown vegetable crop. disease samples showing yellow mosaic symptoms on leaves and pods were collected from himachal pradesh and inoculated on common bean cv. jawala through sap inoculation. the virus successfully transmitted by mechanical inoculation produced yellow mosaic, leaf distortion, curling, wrinkling of leaves followed by stunting of plants. the identity of the virus as bean yellow mosaic virus (bymv) was established throug ...201526645043
apple latent spherical virus vector as vaccine for the prevention and treatment of mosaic diseases in pea, broad bean, and eustoma plants by bean yellow mosaic virus.we investigated the protective effects of a viral vector based on an apple latent spherical virus (alsv) harboring a segment of the bean yellow mosaic virus (bymv) genome against mosaic diseases in pea, broad bean, and eustoma plants caused by bymv infection. in pea plants pre-inoculated with the alsv vaccine and challenge inoculated with bymv expressing green fluorescence protein, bymv multiplication occurred in inoculated leaves, but was markedly inhibited in the upper leaves. no mosaic sympto ...201425386843
development of a vigs vector based on the β-satellite dna associated with bhendi yellow vein mosaic virus.bhendi yellow vein mosaic virus (bymv) is a monopartite begomovirus with an associated β-satellite. βc1 orf encoded by the β-satellite is the symptom determinant and a strong suppressor of post transcriptional gene silencing. to create a virus induced gene silencing vector based upon the β-satellite associated with byvmv the βc1 orf was replaced with multiple cloning sites. gfp transgene and plant endogenous genes su, pds, pcna and ago1 were cloned into β-satellite based vigs vector. gfp express ...201525169741
split personality of a potyvirus: to specialize or not to specialize?bean yellow mosaic virus (bymv), genus potyvirus, has an extensive natural host range encompassing both dicots and monocots. its phylogenetic groups were considered to consist of an ancestral generalist group and six specialist groups derived from this generalist group during plant domestication. recombination was suggested to be playing a role in bymv's evolution towards host specialization. however, in subsequent phylogenetic analysis of whole genomes, group names based on the original hosts o ...201425148372
plant virology and next generation sequencing: experiences with a generation sequencing is quickly emerging as the go-to tool for plant virologists when sequencing whole virus genomes, and undertaking plant metagenomic studies for new virus discoveries. this study aims to compare the genomic and biological properties of bean yellow mosaic virus (bymv) (genus potyvirus), isolates from lupinus angustifolius plants with black pod syndrome (bps), systemic necrosis or non-necrotic symptoms, and from two other plant species. when one clover yellow vein virus (c ...201425102175
[influence of bean yellow mosaic virus on metabolism of photosynthetic pigments, proteins and carbohydrates in glycine soja l].this paper presents data on bymv effects on some physiological processes of glycine soja l. cultivated in the right-bank forest-steppe regions. pigment content (chlorophyll a, b and carotenoids), soluble proteins and water soluble carbohydrates were estimated and, as has been shown, are subjected to significant changes as compared with control plants, namely: a decrease in the content of chlorophyll a, b and carotenoids was 64%, 53% and 36% compared with the control plants. the significant incre ...201424800515
new record of association of bean yellow mosaic virus with mosaic disease of vicia faba in india. 201324426268
serological relationships between the cylindrical inclusion proteins of potyviruses.abstract antisera to the cytoplasmic inclusion proteins (cips) of bean yellow mosaic (bymv), clover yellow vein (clyvv), turnip mosaic (tumv), sweet potato feathery mottle (spfmv), and maize dwarf mosaic (mdmv) potyviruses were used to examine the relationships between the cips of 18 potyviruses. the antisera to cips of bymv, clyvv, tumv, and spfmv cross-reacted to most or all of the purified cips tested in western blot assays. the mdmv cip antiserum reacted significantly only to the mdmv and so ...199818944875
biological, serological, and molecular variabilities of clover yellow vein virus.abstract a comparative study was made on the host reactions, serological properties, and nucleotide sequences of the coat protein (cp) gene of 10 clover yellow vein virus (c1yvv) isolates and one bean yellow mosaic virus (bymv) isolate collected from different host plant species and locations in japan. two strains of c1yvv isolates, grouped on the basis of host reactions on chenopodium amaranticolor, c. quinoa, nicotianaclevelandii, n. benthamiana, vicia faba, and trifolium repens, corresponded ...199718945034
biological characteristic and identification of soybean virus isolated from different ukraine examine the presence and level of viral infection, field observations of the soybean crops in the cherkassy, vinnitsa and kyiv regions have been performed. it was established that the diseases in the soybean plants growing in the examined areas have been caused by two major viruses--smv (soybean mosaic virus) and bymv (bean yellow mosaic virus). the results of field observations have been confirmed using light and electron microscopy and elisa.201222545444
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