serotype a and b strains of bean common mosaic virus are two distinct potyviruses.the serological relationships among strains of bean common mosaic virus (bcmv) (genus potyvirus, family potyviridae) were investigated by testing 13 isolates of the 10 known bcmv pathotypes with two monoclonal antibodies and six antisera to bcmv strains. in addition, other properties of serologically distinct bcmv strains were compared. two groups of bcmv strains were obtained by elisa and western blot serology: serotype a contained the bcmv strains nl3, nl5, and nl8 and serotype b contained the ...19921280510
nomenclature and relationships of some brazilian leguminous potyviruses related to bean common mosaic and/or passionfruit woodiness viruses.the main brazilian literature of the last 10 years on potyviruses of leguminous plants related to bean common mosaic virus (bcmv) and/or to passionfruit woodiness virus (pwv) is discussed and summarized. the viruses dealt with are canavalia acronecrosis, mosaico de canavalia, cassia yellow spot, cowpea green vein-banding, cowpea rugose mosaic and cowpea severe mottle. the viruses have similar biological properties, such as a host range restricted mainly to the leguminosae, aphid transmission, se ...19921450754
a proposal for a bean common mosaic subgroup of order to elucidate the taxonomic positions of bean common mosaic virus (bcmv) and blackeye cowpea mosaic virus (b1cmv), several strains of these viruses were compared on the basis of host ranges, antigenic properties established with antisera to virions and to n-terminal peptide domains of their coat proteins, and high performance liquid chromatographic peptide profiles. the comparison includes three strains of bcmv, viz. nl1, nl3 and ny15, four strains of b1cmv, viz. fla, ind, nr, and w, and ...19921450765
serological and biological relationships among viruses in the bean common mosaic virus subgroup.bean common mosaic virus (bcmv), blackeye cowpea mosaic virus (b1cmv), cowpea aphid-borne mosaic virus (cabmv), azuki bean mosaic virus (azmv), and peanut stripe virus (pstv) are five species of the genus potyvirus, family potyviridae which are seed-transmitted in beans or cowpeas. eighteen isolates of bcmv, five isolates of b1cmv, four isolates of cabmv, and one isolate each of azmv, and pstv were compared serologically using a panel of 13 monoclonal antibodies (mabs) raised against bcmv, b1cmv ...19921450766
strains of bean common mosaic virus consist of at least two distinct potyviruses.bean common mosaic virus (bcmv) consists of a large number of pathotypes and strains which have largely been identified by their characteristic interactions with a selected number of differential bean cultivars. the relationships among these strains and other potyviruses that infect legumes are complex, with indications that bcmv, blackeye cowpea mosaic virus (blcmv) and azuki bean mosaic virus (azmv) may be strains of the one virus. using high performance liquid chromatographic peptide profiles ...19921450767
the complete nucleotide sequences of the coat protein cistron and the 3' non-coding region of a newly-identified potyvirus infecting sweetpotato, as compared to those of sweetpotato feathery mottle virus.complementary dna representing 728 nucleotides of the 3' end of the genomic rna of sweetpotato virus g (spv-g) a newly-identified potyvirus infecting sweetpotato, was cloned and sequenced. this sequence was combined with that previously determined for the 5' terminal part of the coat protein cistron of the virus. the whole sequence contained a single open reading frame (orf) of 1065 nucleotide, with the capacity to encode a coat protein of 355 amino acids, significantly larger than that of other ...19947832639
distribution and localization of bean common mosaic virus and bean black root virus in stems of doubly infected bean plants.plants of bean (phaseolus vulgaris) inoculated first on one primary leaf with strain ny15 of bean common mosaic virus, as inducer, and after three days, on the opposite leaf, with the strain nl3 of bean black root virus, as challenger, did not show systemic necrosis characteristic of the latter strain. this interference phenomenon was studied by determining the amount, distribution and localization of both strains in the part of stem between primary leaves and first trifoliolate leaf in both cha ...19947980014
capsid protein properties of cowpea aphid-borne mosaic virus and blackeye cowpea mosaic virus confirm the existence of two major subgroups of aphid-transmitted, legume-infecting potyviruses.a study of the capsid proteins of different legume-infecting potyviruses using specific monoclonal antibodies on immunoblots of crude extracts from infected plants revealed that cowpea aphid-borne mosaic virus (camv) and blackeye cowpea mosaic virus (bicmv) have coat protein m(r) values of 32k and 35k, respectively. immunoblot comparisons of bicmv, peanut stripe mosaic virus (pstv), bean common mosaic virus (bcmv) and azuki bean mosaic virus (azmv) revealed equal reactivity of their 35k coat pro ...19947996147
cowpea aphid borne mosaic virus-morocco and south african passiflora virus are strains of the same potyvirus.high performance liquid chromatography (hplc) profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphid-borne mosaic virus (cabmv). some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus (b1cmv) while other reports have concluded that this relationship is distant. in this report a tryptic digest of the coat protein of cabmv-morocco was compared w ...19948002788
evolutionary relationships among bean common mosaic virus strains and closely related potyviruses.bean common mosaic virus (bcmv) consists of a large number of strains with complex and controversial relationships among them and with other potyviruses that infect legumes. in order to elucidate the bcmv taxonomic pattern and its evolutionary implications, a phylogenetic analysis has been carried out. the analysis of the coat protein gene and 3' non-coding region (ncr) sequences recently obtained by us and other currently available potyviral sequences confirms the clustering of viruses comprise ...19948165868
in vitro destabilization of plant viruses and cdna synthesis.dna copies of a wide range of rna viruses can be made by the direct addition of appropriately treated, purified virus particles to a reverse transcription reaction. therefore, many problems associated with rna isolation can be circumvented. virus particles can be sufficiently destabilized by adjustments of salt content, buffer, ph or by the use of physical force supplied by a freeze/thaw cycle so that rna in sufficient quantity and physical condition is available for the synthesis of in some cas ...19938263116
sequence data to settle the taxonomic position of bean common mosaic virus and blackeye cowpea mosaic virus isolates.the nucleotide sequences of the coat protein genes and 3' non-translated regions (3'-ntrs) of three isolates of bean common mosaic virus (nl1, nl3 and ny15) and one isolate of blackeye cowpea mosaic virus (w) were determined. comparison of these sequences revealed that the coat proteins of nl1, ny15 and w were identical in size (287 amino acids) and exhibited an overall sequence similarity (94 to 97%), and 84 to 98% in their n-terminal regions. furthermore, their 3'-ntrs were very similar in len ...19938409946
the complete nucleotide sequence and genome organization of bean common mosaic virus (nl3 strain).the nucleotide sequences of 3 cdna clones corresponding to entire rna genome of bean common mosaic virus nl3 strain have been determined. the rna is 9612 nucleotides long, excluding a 3'-terminal poly(a) tail. a putative start codon located at nucleotide positions 170-172 initiates one large open reading frame that is terminated with a uaa codon at position 9368-9370. the predicted polyprotein has 3066 amino acids and an m(r) of 340.3 kda. the positions of putative protein cleavage sites have be ...19958607279
frequent occurrence of recombinant potyvirus isolates.we have performed a systematic search for recombination in the region encoding coat protein and the 3' non-translated region in natural isolates of potyviruses, the largest group of plant rna viruses. the presence of recombination, and the localization of the cross-over points, were confirmed statistically, by three different methods. recombination was detected or suspected in 18 out of 109 potyvirus isolates tested, belonging to four out of eight virus species, and was most prevalent in potato ...19968760448
the nucleotide sequence of the coat protein gene and 3' untranslated region of azuki bean mosaic potyvirus, a member of the bean common mosaic virus subgroup.the relationship of azuki bean mosaic potyvirus (azmv) to members of the bean common mosaic virus (bcmv) subgroup has been unclear. degenerate oligonucleotide primers and the polymerase chain reaction were used to amplify and clone the coat protein (cp) gene and 3' untranslated region (utr) of azmv. the deduced amino acid sequence of the cp is 94% identical to that of dendrobium mosaic virus, establishing the two as strains of the same virus. while the cp amino acid identities between azmv and p ...19968870274
molecular detection of bean common mosaic and bean common mosaic necrosis potyviruses and pathogroups.partial nucleotide sequences of selected isolates of bean common mosaic virus (bcmv) and bean common mosaic necrosis virus (bcmnv) were determined. based on these sequences and previously published sequence data, a reverse transcription, polymerase chain reaction (rt-pcr) in combination with restriction endonuclease analyses, was developed for molecular detection of bcmv, bcmnv and some viral pathogroups (pg). specific detection of the two viruses was accomplished by constructing two virus-speci ...19968920828
development of a scar marker linked to the i gene in common bean.two 24-mer scar primers (sw13) were developed from a previously identified 10-mer rapd primer (ow13(690)) linked to the i gene, which conditions resistance to bean common mosaic virus (bcmv) in common bean. linkage between sw13 and the i gene was tested in three f2 populations segregating for both sw13 and the i gene: n84004/michelite (1.0 +/- 0.7 cm), seafarer/ui-114 (1.3 +/- 0.8 cm), and g91201/alpine (5.0 +/- 2.2 cm). sw13 proved to be more specific and reproducible than the ow13(690) rapd ma ...19968983191
phylogenetic analysis of the potyviridae with emphasis on legume-infecting potyviruses.the 3'-terminal nucleotide sequences of thirteen authenticated strains of bean common mosaic virus (bcmv) and one strain of bean common mosaic necrosis virus (bcmnv) were obtained. the regions sequenced included the coat protein coding sequence and 3'-end non-coding region. these data, combined with sequence information from other legume-infecting potyviruses and the potyviridae were used for phylogenetic analysis. evidence is provided for delineation of bcmnv as distinct from bcmv and the inclu ...19979413507
sequences of the coat protein gene of five peanut stripe virus (pstv) strains from thailand and their evolutionary relationship with other bean common mosaic virus sequences.the coat protein gene and part of the 3' non-coding region of five strains of peanut stripe virus (pstv) from thailand have been cloned and sequenced. phylogenetic comparisons of these strains, known as t1, t3, t5, t6 and t7, and related sequences showed that these strains are indeed strains of pstv. further, pstv strains appear to be related to each other according to their geographic origin. that is, the thai strains are more closely related to each other than they are to strains from the usa ...19989787652
properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria.a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ...19989842442
the nucleotide sequence of the 3'-terminal region of dasheen mosaic virus (caladium isolate) and expression of its coat protein in escherichia coli for antiserum production.a caladium isolate of dasheen mosaic virus (dsmv-ch) was cloned as cdna from genomic rna. the sequence of the 3'-terminal 3158 nucleotides, which consisted of the 3'-terminus of the nia gene, the nib gene, the coat protein (cp) gene, and a 246-nucleotide non-coding region, was between 57-68% similar at the nucleotide level and 72-82% similar at the amino acid level when compared with other potyviruses. phylogenetic analysis of aligned, selected potyviral cp sequences indicate that dsmv-ch is sim ...19989930202
three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup.twenty-seven of 29 strains of viruses in the bean common mosaic virus (bcmv) subgroup of legume-infecting potyviruses reacted strongly with one or more of the monoclonal antibodies (mabs) which are known to be specific for epitopes located along the 50 amino acids which constitute the n-terminal end of the viral coat protein. approximately one half of the virus strains reacted with the n-terminal epitope specific (ntes) mab 4g12 which is specific for epitope e/b4, while the other half reacted wi ...199910446651
phylogenetic analysis of two potyvirus pathogens of commercial cowpea lines: implications for obtaining pathogen-derived a prelude to developing engineered resistance to two important potyvirus pathogens of cowpea, a phylogenetic analysis of strains of cowpea aphid-borne mosaic virus (cabmv) and bean common mosaic virus--blackeye cowpea strain (bcmv-b1c) was undertaken. nucleotide sequences for the coat protein genes and 3'-untranslated regions of four cabmv and one bcmv-b1c strains were determined and included in an analysis with published sequences. while all the newly sequenced viruses showed strong homology ...200010766309
the i gene of bean: a dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus bean common mosaic virus.the resistance to the potyvirus bean common mosaic virus (bcmv) conferred by the i allele in cultivars of phaseolus vulgaris has been characterized as dominant, and it has been associated with both immunity and a systemic vascular necrosis in infected bean plants under field, as well as controlled, conditions. in our attempts to understand more fully the nature of the interaction between bean with the i resistance allele and the pathogen bcmv, we carefully varied both i allele dosage and tempera ...200011059494
detection of infectious baboon cytomegalovirus after baboon-to-human liver xenotransplantation.xenotransplantation is considered to be a solution for the human donor shortage. however, there is a potential risk of transmitting animal infections from the transplanted organ. the known transmissibility and clinical significance of human cytomegalovirus (hcmv) infection after allotransplantation led us to evaluate whether baboon cytomegalovirus (bcmv) transmission could occur after a baboon-to-human liver xenotransplant. we examined serial blood samples from a baboon liver recipient and isola ...200111222707
a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family.a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ...200111402861
phenotypic recombination between bean common mosaic and bean common mosaic necrosis potyviruses in vivo.the us-5 strain of bean common mosaic virus (bcmv) and the nl-8 strain of bean common mosaic necrosis virus (bcmnv) are both seedborne potyviruses in common bean (phaseolus vulgaris l). they have contrasting and highly stable biological characteristics which are genetically controlled. bcmv strain us-5 belongs to pathogenicity group iv. bcmnv strain nl-8 belongs to pathogenicity group iii. the two strains have contrasting serological characteristics: nl-8 is serotype a; us-5 is serotype b. when ...200111448021
molecular characterisation of an isolate of dasheen mosaic virus from zantedeschia aethiopica in china and comparisons in the genus potyvirus.the complete nucleotide sequence of an isolate of dasheen mosaic virus from zantedeschia aethiopica in zhejiang province, china, was determined. the 9991 nucleotide genome was typical of the genus potyvirus and phylogenetic analysis showed it to be a member of the bean common mosaic virus subgroup. the 3'-terminal sequence, including the coat protein region, was determined for three further isolates from china and japan. variations in the length and composition of the n-terminus of the coat prot ...200111699967
a mosaic disease of senna hirsuta induced by a potyvirus in nigeria.a virus inducing mosaic and severe leaf malformation, isolated from senna hirsuta in nigeria, was studied. the virus had a rather narrow host range, infecting a few species in caesalpinaceae, chenopodiaceae and fabaceae families. the virus was widespread in southern nigeria with prevalence ranging from 74% to 86.4% in some locations. it was transmitted mechanically and in a non-persistent manner by myzuspersicae, aphis craccivora and a. spiraecola. there was no evidence of transmission by seeds. ...200111719985
activation of cytomegalovirus in pig-to-primate organ xenotransplantation.xenotransplantation of porcine organs carries the risk of reactivation of latent virus in donor and recipient tissues as well as transmission of viruses between species. we have investigated the activation of baboon cytomegalovirus (bcmv) and porcine cmv (pcmv) in a pig-to-primate model of xenotransplantation. tissues originating from a series of six swine-to-baboon composite thymokidney xenotransplants were investigated. four immunosuppressed baboons died (survival range, 7 to 27 days) with the ...200211967290
bean common mosaic virus isolates causing different symptoms in asparagus bean in china differ greatly in the 5'-parts of their genomes.potyvirus isolates from asparagus bean ( vigna sesquipedalis) plants in zhejiang province, china, caused either rugose and vein banding mosaic symptoms (isolate r) or severe yellowing (isolate y) in this host, but were otherwise similar in host range. both isolates were completely sequenced and shown to be isolates of bean common mosaic virus (bcmv). the complete sequences were 9992 (r) or 10062 (y) nucleotides long and shared 91.7% identical nucleotides (93.2% identical amino acids) in their ge ...200212111434
reduced efficacy of ganciclovir against porcine and baboon cytomegalovirus in pig-to-baboon pig-to-baboon xenotransplantation, porcine cytomegalovirus (pcmv) causes viremia, consumptive coagulopathy, and tissue-invasive disease. baboon cytomegalovirus (bcmv) is associated with invasive disease in xenograft recipients. the efficacy of prophylaxis with intravenous ganciclovir (gcv) was studied for prevention of pcmv and bcmv infections in pig-to baboon xenotransplantation. gcv prophylaxis did not alter the incidence of bcmv activation in recipients, but reduced the amount of virus in ...200312919084
molecular analysis of the 3' terminal region of the genome of beet mosaic virus and its relation with other potyviruses.a1633 nucleotides long 3'-terminal sequence of genome of the beet mosaic virus (btmv) ren1 isolate from slovakia, spanning a part of the nib gene, complete capsid protein (cp) gene and a 3'non-coding region was determined. computer-assisted translation resulted in a polypeptide encompassing typical conserved potyviral amino acid (aa) motifs. homology blast search and phylogenetic analysis revealed that btmv was closest to bean common mosaic virus, soybean mosaic virus and zucchini yellow mosaic ...200314505096
the nucleotide sequence of watermelon mosaic virus (wmv, potyvirus) reveals interspecific recombination between two related potyviruses in the 5' part of the genome.watermelon mosaic virus (wmv, potyvirus) is a potyvirus with a worldwide distribution, mostly in temperate and mediterranean regions. according to the partial sequences that were available, wmv appeared to share high sequence similarity with soybean mosaic virus (smv), and it was almost considered as a strain of smv in spite of its different and much broader host range. like smv, it was also related to legume-infecting potyviruses belonging to the " bean common mosaic virus (bcmv) subgroup". in ...200415290385
identification and molecular characterization of zantedeschia mild mosaic virus, a new calla lily-infecting potyvirus.tissue-cultured seedlings of calla lily (zantedeschia spp.) with mild mosaic symptom were observed in taiwan. a new potyvirus, zantedeschia mild mosaic virus (zammv), was identified in the diseased plants based on the result of elisa, virion morphology and sequence data. in the host range test, zammv only infected philodendron selloum and zantedeschia spp. causing systemic mosaic and mild mosaic symptom, respectively. the 3'-terminal region of the virus was amplified by rt-pcr from total rna usi ...200515703845
properties of a virus isolated from vernonia amygdalina del. in lagos, nigeria.a previously uncharacterized virus tentatively named vernonia green vein-banding virus (vgvbv) was isolated from vernonia amygdalina del. ("bitterleaf") from lagos, nigeria. the virus was mechanically transmissible but had a narrow host range restricted to nicotiana benthamiana, chenopodium quinoa and c. amaranticolor. it was also transmissible in a non-persistent manner by myzus persicae. the virus was purified from n. benthamiana and about 750 nm long flexuous rod-shaped particles were observe ...200415745049
resistance conferred against bean common mosaic virus by the incompletely dominant i locus of phaseolus vulgaris is active at the single cell level.a protoplast transfection system was used in phaseolus vulgaris to study the incompletely dominant resistance locus i. the genetic materials in the study were cultivar 'black turtle soup' lines nearly isogenic for i and their f1. accumulation of bean common mosaic virus (bcmv; genus potyvirus) rna and virions was assayed following bcmv rna electrotransfection of protoplasts from each genotype. bcmv rna and virions accumulated in all genotypes tested but the relative rates of rna accumulation dif ...200516132184
genetic and molecular characterization of the i locus of phaseolus vulgaris.the i locus of the common bean, phaseolus vulgaris, controls the development of four different phenotypes in response to inoculation with bean common mosaic virus, bean common mosaic necrosis virus, several other related potyviruses, and one comovirus. we have generated a high-resolution linkage map around this locus and have aligned it with a physical map constructed with bac clones. these clones were obtained from a library of the cultivar "sprite," which carries the dominant allele at the i l ...200616322513
detection and characterisation of a second potyvirus from thunberg fritillary in china.plants of thunberg fritillary (fritillaria thunbergii miq.) from zhejiang province, were found to be co-infected with two distinct potyviruses. one was an isolate of the recently reported thunberg fritillary mosaic virus (tfmv; wei et al., (2005) arch virol 150: 1271-1280), while the other was a distinct virus that did not react with tfmv antiserum nor with antisera to 17 other potyviruses, except for a weak reaction with antibodies produced to soybean mosaic virus (smv) pinellia strain. both vi ...200616328133
differential gene expression in phaseolus vulgaris i locus nils challenged with bean common mosaic virus.the phaseolus vulgaris i locus-bean common mosaic virus (bcmv; potyviridae) pathosystem is of critical importance to bean geneticists, breeders and pathologists because of the worldwide distribution of both the virus and germplasm containing this resistance gene. in order to learn more about the molecular responses characteristic of this resistance gene, a cdna-aflp screen was conducted on homozygous nils of p. vulgaris variety 'black turtle soup' (bt), containing either the i locus allele for r ...200616568285
the genomic sequence of wisteria vein mosaic virus and its similarities with other potyviruses.the complete nucleotide sequence of a beijing isolate of wisteria vein mosaic virus was determined to be 9695 nucleotides in length excluding the poly(a) tail. sequence analysis predicted a single large open reading frame of 9279 nucleotides potentially encodes a polyprotein of 3092 amino acids. phylogenetic analysis based on the genomic and deduced amino acid sequences support the current status of wisteria vein mosaic virus (wvmv) as a distinct virus of the genus potyvirus and a member of the ...200616732423
the complete nucleotide sequence of a pakistani isolate of watermelon mosaic virus provides further insights into the taxonomic status in the bean common mosaic virus subgroup.watermelon mosaic virus (wmv) is a potyvirus with a worldwide distribution, but is mostly found in temperate and mediterranean regions. the complete nucleotide (nt) sequence of a pakistani isolate of wmv (wmv-pk) was determined and compared with french isolate (wmv-fr) and other closely related potyviruses. wmv-pk showed overall identities of 94.4% (nt) and 96% (amino acid; aa) with the wmv-fr. however, variability was observed in the 5' utr and p1 region. although sequence identities over most ...200616732483
genetic mapping of the bean golden yellow mosaic geminivirus resistance gene bgm-1 and linkage with potyvirus resistance in common bean (phaseolus vulgaris l.).bean golden yellow mosaic virus (bgymv) is a whitefly-transmitted geminivirus of the begomovirus family that causes important yield losses to common beans grown in tropical and sub-tropical countries of latin america and the caribbean. a major resistance gene that has been widely deployed in this region is the recessive locus bgm-1 that prevents the development of severe yellowing typical of the disease. in this study, we developed a co-dominant sequence-characterized amplified region (scar) mar ...200717115130
a genetic linkage map of phaseolus vulgaris l. and localization of genes for specific resistance to six races of anthracnose (colletotrichum lindemuthianum).a genetic map of common bean was constructed using 197 markers including 152 rapds, 32 rflps, 12 scars, and 1 morphological marker. the map was established by using a f(2) population of 85 individuals from the cross between a line derived from the spanish landrace andecha (andean origin) and the mesoamerican genotype a252. the resulting map covers about 1,401.9 cm, with an average marker distance of 7.1 cm and includes molecular markers linked to disease resistance genes for anthracnose, bean co ...200717186216
recombination and gene duplication in the evolutionary diversification of p1 proteins in the family potyviridae.genome structure and sequence are notably conserved between members of the family potyviridae. however, some genomic regions of these viruses, such as that encoding the p1 protein, show strikingly high variability. in this study, some partially conserved motifs were identified upstream of the quite well-conserved protease domain located near the p1 c terminus. the irregular distribution of these motifs suggests that the potyviral p1 proteins have undergone complex evolutionary diversification. e ...200717325376
strains of bcmv and bcmnv characterized from lima bean plants affected by deforming mosaic disease in peru.viruses of the species bean common mosaic virus (bcmv) and bean common mosaic necrosis virus (bcmnv) were simultaneously detected by the different size of pcr amplicons in lima bean plants (phaseolus lunatus) displaying deforming mosaic symptoms in peru. phylogenetic analysis of partial deduced cp amino acid sequences indicated that the peruvian bcmv isolates belong to new strains. one isolate differed from the other peruvian isolates, and also from the ten previously described bcmv strains reco ...200717564759
identification and sequence analysis of potyviruses infecting crops in vietnam.fifty-two virus isolates from 13 distinct potyvirus species infecting crops in vietnam were identified and the 3' region of each genome was sequenced. the viruses were: bean common mosaic virus (bcmv), potato virus y (pvy), sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv), chilli veinal mottle virus (chivmv), zucchini yellow mosaic virus (zymv), leek yellow stripe virus (lymv), shallot yellow stripe virus (sysv), onion yellow dwarf virus (oydv), turnip mosaic virus (tumv), dasheen mosa ...200817906829
design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses.two pairs of degenerate primers were designed from sequences within the potyviral ci (cifor/cirev) and hc-pro-coding regions (hpfo/hprev), and these were shown to be highly specific to members of the genus potyvirus. using the cifor and cirev primers, three novel potyviruses infecting crop and weed species from vietnam were detected, namely telosma mosaic virus (telmv) infecting telosma (telosma cordata, asclepiadaceae), peace lily mosaic virus (pelmv) infecting peace lily (spathiphyllum patinii ...200817906831
the potyviruses of australia.many potyviruses have been found in australia. we analyzed a selected region of the coat protein genes of 37 of them to determine their relationships, and found that they fall into two groups. half were isolated from cultivated plants and crops, and are also found in other parts of the world. sequence comparisons show that the australian populations of these viruses are closely related to, but less variable than, those in other parts of the world, and they represent many different potyvirus line ...200818566735
towards a system for the identification and classification of potyviruses. ii. virus particle length, symptomatology, and cytopathology of six distinct viruses.the mean particle lengths of lettuce mosaic virus (lmv), bean common mosaic virus (bcmv), passionfruit woodiness virus (pwv), potato virus y (pvy), sugarcane mosaic virus (scmv), and one strain of bean yellow mosaic virus (bymv) were all shown to be close to 750 nm. however, the length of two other serologically closely related strains of bymv, variants of pea mosaic virus referred to as pmv and spmv, had significantly longer particles, about 792 and 842 nm. each of the distinct viruses had read ...198118635068
genetic characterization of differential reactions among host group 3 common bean cultivars to nl-3 k strain of bean common mosaic necrosis virus.abstract a previously unrecognized recessive resistance gene (or allele) was identified in three host group (hg) 3 common bean (phaseolus vulgaris) cvs. olathe, victor, and ui 37, based on genetic analysis of plants from five populations screened with the nl-3 k strain of bean common mosaic necrosis virus (bcmnv). the gene (or allele) was associated with resistance to leaf stunting and deformity and reduction in plant height. the gene (or allele) provides similar, but slightly better resistance ...200318943054
nl-3 k strain is a stable and naturally occurring interspecific recombinant derived from bean common mosaic necrosis virus and bean common mosaic virus.abstract a strain of bean common mosaic necrosis virus (bcmnv) from idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the nl-3 d strain (of drifjhout) by reaction of differential bean cultivars. however, this bcmnv strain (designated nl-3 k) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, and 5. the nucleotide sequence encoding the predicted polyprotein of nl-3 k was 9,893 nucleotides (nt) i ...200518943301
genotyping common bean for the potyvirus resistance alleles i and bc-1(2) with a multiplex real-time polymerase chain reaction assay.abstract a multiplex real-time polymerase chain reaction (pcr) assay was developed to simultaneously genotype plants for the i and bc-1(2) alleles, which condition resistance in beans to bean common mosaic virus and bean common mosaic necrosis virus. a segregating f(2) population was derived from the cross between pinto bean breeding line p94207-189a (bc-1 bc-1 i i) and olathe (bc-12 bc-1(2) i i). real-time pcr assays were developed that were specific for each allele, and a multiplex pcr reactio ...200518943314
the bean common mosaic virus lineage of potyviruses: where did it arise and when?there are more than 30 species in the bean common mosaic virus lineage of the genus potyvirus. we have used their partial coat protein gene sequences to infer their phylogenies and have compared these with host and provenance information. members of six species of the lineage have been isolated from crops distributed around the world, but three of these show clear links with south and east asia. members of the remaining species have been found in wild plants, minor crop species or ornamentals, a ...200819030956
rhizobacteria-mediated resistance against the blackeye cowpea mosaic strain of bean common mosaic virus in cowpea (vigna unguiculata).the present study investigated the effect of seven bacillus-species plant-growth-promoting rhizobacteria (pgpr) seed treatments on the induction of disease resistance in cowpea against mosaic disease caused by the blackeye cowpea mosaic strain of bean common mosaic virus (bcmv).200919569083
mapping of qtls for morpho-agronomic and seed quality traits in a ril population of common bean (phaseolus vulgaris l.).the objective of this research was to determine the quantitative trait loci (qtls) controlling phenological traits (days to flowering, days to end of flowering, days to harvest as green pod, and days to maturity), seed size traits (seed length, seed height, seed width, and seed weight), and seed quality traits (water absorption, and coat proportion), in common bean. a population of 104 f(7) recombinant inbred lines (rils) derived from an inter-gene pool cross between xana, and cornell 49242, was ...201020084493
the complete genome sequence of freesia mosaic virus and its relationship to other potyviruses.we have completed the genomic sequence of a potyvirus, freesia mosaic virus (fremv), and compared it to those of other known potyviruses. the full-length genome sequence of fremv consists of 9,489 nucleotides. the large protein contains 3,077 amino acids, with an aug start codon and uaa stop codon, containing one open reading frame typical of a potyvirus polyprotein. the polyprotein of fremv-kr gives rise to eleven proteins (p1, hc-pro, p3, pipo, 6k1, ci, 6k2, vpg, nia, nib and cp), and putative ...201020401501
potyviral resistance derived from cultivars of phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eif4e allele.eukaryotic translation initiation factors (eifs) play a central role in potyviral infection. accordingly, mutations in the gene encoding eif4e have been identified as a source of recessive resistance in several plant species. in common bean, phaseolus vulgaris, four recessive genes, bc-1, bc-2, bc-3 and bc-u, have been proposed to control resistance to the potyviruses bean common mosaic virus (bcmv) and bean common mosaic necrosis virus. in order to identify molecular entities for these genes, w ...201020447274
identification of a novel soybean mosaic virus isolate in china that contains a unique 5' terminus sharing high sequence homology with bean common mosaic virus.two soybean viral isolates 4469-4 and sc6 from plants in china were characterized by serological assays, pathogenicity tests, full-genome sequencing and sequence analyses. both isolates are determined to be soybean mosaic virus (smv) isolates but 4469-4 is different from other known smvs by inducing symptoms on common bean. 4469-4 has an rna genome of 9994 nucleic acids (nt) encoding 3202 amino acids (aa), which is approximately 400 nt longer than that of sc6 and other smv strains. comparison wi ...201121262287
visualization of resistance responses in phaseolus vulgaris using reporter tagged clones of bean common mosaic virus.reporter tagged virus clones can provide detailed information on virus-host interactions. in phaseolus vulgaris (bean), four recessive and one dominant gene are known to control infection by strains of the potyvirus species bean common mosaic virus (bcmv). to study the interactions between bcmv and bean genotypes with different resistance gene combinations, an infectious clone of the strain ru1 was tagged with the uida gene encoding β-glucuronidase (gus). the clone was agroinoculated to bean gen ...201121549773
indigenous and introduced potyviruses of legumes and passiflora spp. from australia: biological properties and comparison of coat protein nucleotide sequences.five australian potyviruses, passion fruit woodiness virus (pwv), passiflora mosaic virus (pamv), passiflora virus y, clitoria chlorosis virus (clcv) and hardenbergia mosaic virus (harmv), and two introduced potyviruses, bean common mosaic virus (bcmv) and cowpea aphid-borne mosaic virus (cabmv), were detected in nine wild or cultivated passiflora and legume species growing in tropical, subtropical or mediterranean climatic regions of western australia. when clcv (1), pamv (1), pavy (8) and pwv ...201121744001
analysis of 3'-terminal region of bean common mosaic virus strains infecting common bean in india.four strains (nl-1, nl-1n, nl-7 and nl-7n) of bean common mosaic virus (bcmv) prevalent on common bean (phaseolus vulgaris) in himachal pradesh, a north-western himalayan state of india were compared at the 3' terminal region of the viral genome to elucidate variation and relationship among these strains. 3' terminal region comprising of partial nuclear inclusion b (nib) (171-233 bp), complete coat protein (cp) (860 bp) and 3' untranslated region (utr) (208-244 bp) shared 96-98% nucleotide and 9 ...201123637500
simultaneous detection of both rna and dna viruses infecting dry bean and occurrence of mixed infections by bgymv, bcmv and bcmnv in the central-west region of mexico.a multiplex reverse transcription polymerase chain reaction (rt-pcr) assay was developed to simultaneously detect bean common mosaic virus (bcmv), bean common mosaic necrotic virus (bcmnv), and bean golden yellow mosaic virus (bgymv) from common bean leaves dried with silica gel using a single total nucleic acid extraction cetyl trimethyl ammonium bromide (ctab) method. a mixture of five specific primers was used to amplify three distinct fragments corresponding to 272 bp from the ac1 gene of bg ...201728358318
bean common mosaic virus and bean common mosaic necrosis virus: relationships, biology, and prospects for control.the closely related potyviruses bean common mosaic virus (bcmv) and bean common mosaic necrosis virus (bcmnv) are major constraints on common bean (phaseolus vulgaris) production. crop losses caused by bcmv and bcmnv impact severely not only on commercial scale cultivation of this high-value crop but also on production by smallholder farmers in the developing world, where bean serves as a key source of dietary protein and mineral nutrition. in many parts of the world, progress has been made in c ...201526111585
a recombinant of bean common mosaic virus induces temperature-insensitive necrosis in an i gene-bearing line of common bean.the i gene is a single, dominant gene conferring temperature-sensitive resistance to all known strains of bean common mosaic virus (bcmv) in common bean (phaseolus vulgaris). however, the closely related bean common mosaic necrosis virus (bcmnv) induces whole plant necrosis in i-bearing genotypes of common bean, and the presence of additional, recessive genes is required to prevent this severe whole plant necrotic reaction caused by bcmnv. almost all known bcmnv isolates have so far been classif ...201424875385
metagenomic-based screening and molecular characterization of cowpea-infecting viruses in burkina faso.cowpea, (vigna unguiculata l. (walp)) is an annual tropical grain legume. often referred to as "poor man's meat", cowpea is one of the most important subsistence legumes cultivated in west africa due to the high protein content of its seeds. however, african cowpea production can be seriously constrained by viral diseases that reduce yields. while twelve cowpea-infecting viruses have been reported from africa, only three of these have so-far been reported from burkina faso. here we use a virion- ...201627764211
dynamic transcriptome profiling of bean common mosaic virus (bcmv) infection in common bean (phaseolus vulgaris l.).bean common mosaic virus (bcmv) is widespread, with phaseolus species as the primary host plants. numerous bcmv strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. the molecular responses in phaseolus to bcmv infection have not yet been well characterized.201627515794
bean common mosaic virus isolate exhibits a novel pathogenicity profile in common bean, overcoming the bc-3 resistance allele coding for the mutated eif4e translation initiation factor.resistance against bean common mosaic virus (bcmv) in phaseolus vulgaris is governed by six recessive resistance alleles at four loci. one of these alleles, bc-3, is able to protect p. vulgaris against all bcmv strains and against other potyviruses; bc-3 was identified as the eif4e allele carrying mutated eukaryotic translation initiation factor gene. here, we characterized a novel bcmv isolate 1755a that was able to overcome bc-2 and bc-3 alleles in common bean. thus, it displayed a novel patte ...201526196181
application of in silico bulked segregant analysis for rapid development of markers linked to bean common mosaic virus resistance in common bean.common bean was one of the first crops that benefited from the development and utilization of molecular marker-assisted selection (mas) for major disease resistance genes. efficiency of mas for breeding common bean is still hampered, however, due to the dominance, linkage phase, and loose linkage of previously developed markers. here we applied in silico bulked segregant analysis (bsa) to the beancap diversity panel, composed of over 500 lines and genotyped with the barcbean_3 6k snp beadchip, t ...201425326146
a genomic survey of thirty soybean-infecting bean common mosaic virus (bcmv) isolates from china pointed bcmv as a potential threat to soybean production.widely known as a severe pathogen of bean plants, the bean common mosaic virus (bcmv) has been reported to infect soybeans only sporadically and the involved strains were all found in china regions. to explore variations among soybean-infecting bcmv strains, hundreds of soybean mosaic leave samples were collected throughout china, with a total of 30 bcmv isolates detected and their genomes sequenced. these newly obtained genomes, together with 16 other bcmv genomes available in genbank were exam ...201425107622
recombinants of bean common mosaic virus (bcmv) and genetic determinants of bcmv involved in overcoming resistance in common bean.bean common mosaic virus (bcmv) exists as a complex of strains classified by reactions to resistance genes found in common bean (phaseolus vulgaris); seven bcmv pathotypes have been distinguished thus far, numbered i to vii. virus genetic determinants involved in pathogenicity interactions with resistance genes have not yet been identified. here, we describe the characterization of two novel field isolates of bcmv that helped to narrow down these genetic determinants interacting with specific p. ...201424915430
histochemical visualization of ros and antioxidant response to viral infections of vegetable crops grown in azerbaijan.extremes of environmental conditions, such as biotic stresses, strongly affect plant growth and development and may adversely affect photosynthetic process. virus infection is especially problematic in crops, because unlike other diseases, its impact cannot be reduced by phytosanitary treatments. the vegetable crops (solanum lycopеrsicum l, cucurbita melo l., cucumis sativus l., piper longum l., solánum melongéna l., vicia faba l.) showing virus-like symptoms were collected from fields located i ...201424661407
hardenbergia mosaic virus: crossing the barrier between native and introduced plant species.hardenbergia mosaic virus (harmv), genus potyvirus, belongs to the bean common mosaic virus (bcmv) potyvirus lineage found only in australia. the original host of harmv, hardenbergia comptoniana, family fabaceae, is indigenous to the south-west australian floristic region (swafr), where lupinus spp. are grown as introduced grain legume crops, and exist as naturalised weeds. two plants of h. comptoniana and one of lupinus cosentinii, each with mosaic and leaf deformation symptoms, were sampled fr ...201424594521
recombinant soybean mosaic virus is prevalent in chinese soybean fields.recombinant soybean mosaic virus (smv-r) is a novel strain that has recently been identified. smv-r was first isolated from chongqing, china, and exhibits different pathogenicity on soybeans and common beans compared with normal soybean mosaic virus (smv-n). smv-r arose from a recombination event between smv and bean common mosaic virus (bcmv) or a bcmv-like virus. in this study, we assessed the prevalence of smv-r in chinese soybean fields. polymerase chain reaction results showed that smv-r wa ...201424445813
cross-kingdom sequence similarities between human micro-rnas and plant viruses.micro-rnas regulate the expression of cellular and tissue phenotypes at a post-transcriptional level through a complex process involving complementary interactions between micro-rnas and messenger-rnas. similar nucleotide interactions have been shown to occur as cross-kingdom events; for example, between plant viruses and plant micro-rnas and also between animal viruses and animal micro-rnas. in this study, this view is expanded to look for cross-kingdom similarities between plant virus and huma ...201324228136
immunological detection of bean common mosaic virus in french bean (phaseolus vulgaris l.) leaves.bean common mosaic potyvirus (bcmv) is an important seed borne pathogen of french bean. differential inoculation with bean common mosaic virus at cotylodonary trifoliate leaf stage and pre-flowering stage of crop growth revealed that cotyledonary leaf infection favored maximum disease expression. under immunosorbent electron microscopy (isem) the virus particles of filamentous structure having a diameter of 750 nm (l) and 15 nm (w) were observed. these particles gave positive precipitin tests wi ...201023100839
towards an integrated linkage map of common bean 2. development of an rflp-based linkage map.a restriction fragment length polymorphism (rflp)-based linkage map for common bean (phaseolus vulgaris l.) covering 827 centimorgans (cm) was developed based on a f2 mapping population derived from a cross between bat93 and jalo eep558. the parental genotypes were chosen because they exhibited differences in evolutionary origin, allozymes, phaseolin type, and for several agronomic traits. the segregation of 152 markers was analyzed, including 115 rflp loci, 7 isozyme loci, 8 random amplified po ...199324195923
inheritance of resistance to potato y viruses in phaseolus vulgaris l : 1. two independent genes for resistance to watermelon mosaic virus-2.resistance to watermelon mosaic virus-2 in phaseolus vulgaris l. is conferred by two distinct dominant alleles at independent loci. based on segregation data one locus is designated wmv, the other, hsw. the dominant allele wmv from cv. great northern 1140 prevents systemic spread of the virus but viral replication occurs in inoculated tissue. in contrast, hsw confers both local and systemic resistance to wmv-2 below 30c. at higher temperatures, plants that carry this allele in the absence of mod ...198724240215
detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia.a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ...201728855829
robust rnai-mediated resistance to infection of seven potyvirids in soybean expressing an intron hairpin nib rna.viral pathogens, such as soybean mosaic virus (smv), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. engineering resistance by rnai-mediated gene silencing is a powerful strategy for controlling viral diseases. in this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, nib) gene was isolated from the smv sc3 strain, driven by the leaf-specific rbcs2 promoter from phaseolus vulgaris, and introduced into soybean. th ...201728840434
rnai-mediated smv p3 cistron silencing confers significantly enhanced resistance to multiple potyvirus strains and isolates in transgenic soybean.robust rnai-mediated resistance to multiple potyvirus strains and isolates, but not to secovirus bpmv, was conferred by expressing a short smv p3 hairpin in soybean plants. engineering resistance to multiple potyvirus strains is of great interest because of a wide variability of the virus strains, and mixed infections of multiple viruses or strains commonly associated with field grown soybean. in this study, rnai-mediated silencing of the soybean mosaic virus (smv) p3 cistron, which is reported ...201728756582
Displaying items 1 - 81 of 81