| the nucleotide sequence and genome organization of strawberry mild yellow edge-associated potexvirus. | the nucleotide sequence (5966 nucleotides) of cdna clones of strawberry mild yellow edge-associated potexvirus was determined. the genome contains six open reading frames (orfs) encoding putative proteins with mrs of 149,423, 25,344, 11,576, 8079, 25,714 and 11,216. in the first three putative proteins and the coat protein considerable similarity was found to comparable polypeptides of the potexviruses potato virus x, clover yellow mosaic virus, narcissus mosaic virus, papaya mosaic virus, white ... | 1992 | 1339469 |
| a new potexvirus associated with strawberry mild yellow edge disease. | a physical map of cdna clones prepared from dsrna associated with the my-18 source of strawberry mild yellow edge (smye) was constructed and 854 nucleotides adjacent to the 3' poly(a) tail were sequenced. the larger open reading frame product of mr 25714 showed considerable amino acid homology to the coat protein cistrons of six potexviruses and two carlaviruses. a second product of mr 11216 encoded completely within the coat protein cistron, but in a different frame, has similarities to two pot ... | 1990 | 2191082 |
| phylogenetic analysis of triple gene block viruses based on the tgb 1 homolog gene indicates a convergent evolution. | the complete nucleotide sequence of the triple gene block one (tgb 1) of cymbidium mosaic potexvirus (cymmv) was compared to those from other potex-, carla-, furo- and hordeiviruses. seven conserved motifs in the tgb 1, including the atp-gtp binding domain (p-loop) consensus gxxgktsts, were found in all four virus genera. we propose that all tgbv can be classified into phylogenetic clusters based on their tgb 1 homolog genes. these clusters can be further delineated to form subgroups. the first ... | 1998 | 9654683 |
| multiplex rt-pcr detection of four aphid-borne strawberry viruses in fragaria spp. in combination with a plant mrna specific internal control. | the principal aphid-borne viruses infecting strawberry (fragaria spp.) strawberry crinkle virus (scv), strawberry mild yellow edge virus (smyev), strawberry mottle virus (smov) and strawberry vein banding virus (svbv) can cause serious crop losses. in this paper, a multiplex reverse transcriptase polymerase chain reaction (rt-pcr) method is described for the simultaneous detection of all four viruses in combination with a plant mrna specific internal control which can be used as an indicator of ... | 2003 | 12880923 |
| the complete sequence of the genomic rna of an isolate of lily virus x (genus potexvirus). | the complete sequence of the genomic rna of an isolate of lily virus x (lvx) has been determined for the first time. the isolate from the netherlands was 5823 nucleotide (nt) long excluding the 3'-poly(a) tail, making it the shortest reported potexvirus sequence. the 5'-non-coding region begins with ggaaaa like that of scallion virus x (scavx) and some isolates of cymbidium mosaic virus (cymmv), whereas those of other sequenced potexviruses probably all begin with gaaaa. the genome organisation ... | 2004 | 15578239 |
| strain diversity and conserved genome elements in strawberry mild yellow edge virus. | the complete nucleotide sequence of strawberry mild yellow edge virus isolate d74, and the sequences of a 878 nt region of the coat protein and flanking regions of twenty three isolates of smyev were obtained and analysed. the full sequence of the aphid transmissible strain d74 was deduced and found to have an 86% sequence identity to the non-transmissible agrobacterium infectious my18 strain. in contrast to isolate my18 the 5' terminal nucleotides (gaaaac) of d74 are typical of those from other ... | 2004 | 15669103 |
| genetic structure of the aphid, chaetosiphon fragaefolii, and its role as a vector of the strawberry yellow edge virus to a native strawberry, fragaria chiloensis in chile. | the monoecious anholocyclical aphid, chaetosiphon fragaefolii (cockerell) (homoptera: aphididae), was collected on a native strawberry, fragaria chiloensis (l.) duchesne (rosales: rosaceae) from different sites in chile. the presence of this aphid was recorded during two consecutive years. f. chiloensis plants were collected from seven natural and cultivated growing areas in central and southern chile. aphids were genotyped by cross-species amplification of four microsatellite loci from other ap ... | 2012 | 23438175 |
| mint virus x: a novel potexvirus associated with symptoms in 'variegata' mint. | mentha x gracilis 'variegata', an ornamental mint clone first described about 200 years ago, exhibits virus-like vein banding symptoms. double-stranded rna and virion isolations revealed the presence of three viruses in a 'variegata' plant. cloning and sequencing disclosed that one of the viruses was a previously unidentified species with similarities to members of the flexiviridae family, designated as mint virus x (mvx). the complete nucleotide sequence of the virus was determined. phylogeneti ... | 2006 | 16096710 |
| infectious cdna clone used to identify strawberry mild yellow edge-associated potexvirus as causal agent of the disease. | a full-length in vivo infectious cdna clone of strawberry mild yellow edge-associated potexvirus (smyeav) was constructed and used to inoculate fragaria vesca 'alpine' seedlings and rubus rosifollus. both host plants could be infected using particle bombardment or agroinoculation, but not by mechanical inoculation. a method that used potted strawberry plants for particle bombardment resulted in high survival and infection rates. the plants developed systemic infection and virus particles were de ... | 1997 | 9292025 |