| control and expression of 3' open reading frames in clover yellow mosaic virus. | the genomic rna of clover yellow mosaic virus (cymv) contains at least seven open reading frames (orfs) which are organized in a more elaborate array than in other sequenced members of the potexvirus group. we have investigated the strategy by which orfs located in the 3' region of cymv's genomic rna are differentially expressed by correlating the location of the 5' termini of the two abundant viral subgenomic rnas with their coding potential. we have mapped the 5' termini of the subgenomic rnas ... | 1990 | 2238463 |
| electron microscopy of intracellular aggregates in pea (pisum sativum) infected with clover yellow mosaic virus. | | 1966 | 5943538 |
| genome characterization and taxonomy of plantago asiatica mosaic potexvirus. | the complete nucleotide sequence of plantago asiatica mosaic virus (p1amv) genomic rna has been determined. the 6128 nucleotide sequence contains five open reading frames (orfs) coding for proteins of m(r) 156k (orf1), 25k (orf2), 12k (orf3), 13k (orf4) and 22k (orf5). the sequences of these p1amv proteins exhibit strong homology to the proteins of the other potexviruses. phylogenetic trees based on the multiple sequence alignments of three conserved domains in orf1 product and capsid protein re ... | 1994 | 8113746 |
| a mosaic disease of senna hirsuta induced by a potyvirus in nigeria. | a virus inducing mosaic and severe leaf malformation, isolated from senna hirsuta in nigeria, was studied. the virus had a rather narrow host range, infecting a few species in caesalpinaceae, chenopodiaceae and fabaceae families. the virus was widespread in southern nigeria with prevalence ranging from 74% to 86.4% in some locations. it was transmitted mechanically and in a non-persistent manner by myzuspersicae, aphis craccivora and a. spiraecola. there was no evidence of transmission by seeds. ... | 2001 | 11719985 |
| detection of strain-specific serological activity in antisera of tobacco mosaic virus, clover yellow mosaic virus, and potato virus x, by complement fixation. | | 1963 | 14001977 |
| nucleotide sequence and in vitro translation of the coat protein gene of cymbidium mosaic virus. | the nucleotide sequence of the coat protein gene of cymbidium mosaic virus (cymv) has been determined from a cdna clone that encompassed the 3'-region of the virus genome. the 0.75 kb coat protein open reading frame (orf) codes for a 24kd polypeptide. the coat protein coding region was separated from the 3'-poly(a) terminal of the viral genome by an 80 base noncoding region. the derived amino acid sequence of this orf showed considerable homology with other potexvirus coat protein sequences, not ... | 1993 | 8103614 |
| cloning and sequencing of a cdna encoding the coat protein of a korean isolate of cymbidium mosaic virus. | the 3'-terminal nucleotide (nt) sequence of the coat protein (cp)-encoding gene of the korean isolate of cymbidium mosaic potexvirus (cymv-k) has been determined. the sequence contained a full-length orf coding for the viral cp. it comprises 663 nt and a polypeptide chain of 220 amino acids with a m(r) of 23,718. the stop codon (tga) is followed by a 3'-untranslated region (3'-utr) of 79 nt and a poly(a) tract. the putative polyadenylation signal, aataaa motif, is found in the terminus of the 3' ... | 1995 | 7758973 |
| viral protein in early stages of clover yellow mosaic virus infection of vicia faba. | | 1971 | 4108186 |
| single- and double-stranded viral rnas in plants infected with the potexviruses papaya mosaic virus and foxtail mosaic virus. | three classes of viral rna were recovered from polyribosomes purified from papaya leaves infected with papaya mosaic virus (papmv) and from barley leaves infected with foxtail mosaic virus (fomv): full-length viral rnas [6.8 and 6.2 kilobases (kb), respectively]; less abundant intermediate subgenomic rnas (2.2 and 1.9 kb), and abundant, small subgenomic rnas (1 and 0.9 kb). small amounts of the papmv-specified 1.0-kb subgenomic rna were encapsidated, whereas no encapsidated subgenomic rnas could ... | 1988 | 3182231 |
| nucleotide sequence of the 3'-terminal region of clover yellow mosaic virus rna. | the nucleotide sequence of the 3'-terminal region of clover yellow mosaic virus rna determined from cdna clones contains one major open reading frame (orf) encoding a protein with an mr of 28,093 (28.1 k). a smaller orf, in frame with the major one, is also present and encodes a 23.5k protein which is similar in size to the capsid protein of the virus and has several sequence similarities with the coat proteins of four other potexviruses. the smaller orf is probably used for the expression of th ... | 1989 | 2738582 |
| complete nucleotide sequence of clover yellow mosaic virus rna. | the entire genomic rna of clover yellow mosaic virus was sequenced from cdna clones and run-off cdna transcripts. the genomic rna is 7015 nucleotides in length [excluding a 3' poly(a) tail], with six open reading frames (orfs) greater than 150 nucleotides in length. the first five orfs encode proteins of mr 191k, 26k, 12k, 6.5k and 28k, respectively. the sixth orf lies completely within orf1 and codes for a protein of mr 14k. the capsid protein coding region (mr 23k) is found within orf5 which e ... | 1990 | 2212986 |
| defective rnas of clover yellow mosaic virus encode nonstructural/coat protein fusion products. | a small group of 1.2-kb rnas present on polyribosomes from clover yellow mosaic virus (cymv)-infected tissue contains sequences from the genomic rna (grna) of cymv and is encapsidated by cymv coat protein. some features of these rnas suggest that they are similar to defective interfering (di) rnas, and would be the first to be reported for the potexvirus group. the prototype 1.2-kb rna is 1172 nucleotides in length excluding a probable poly(a) tail and is composed of two noncontiguous regions co ... | 1991 | 1830181 |
| complete genome sequence of a new bipartite begomovirus infecting cotton in the republic of benin in west africa. | here, we report the complete genome sequence of a novel bipartite begomovirus isolated from cotton plants (gossypium raimondii, malvaceae) exhibiting light yellow mosaic symptoms. the genome sequence was determined by illumina dna sequencing and confirmed by sanger sequencing of rca-enriched, cloned circular genomic components. the dna-a and dna-b components were each ~2.7 kb in size, and their genome arrangement was characteristic of other old world bipartite begomoviruses. while the dna-a comp ... | 2016 | 27224982 |
| complete nucleotide sequences of two begomoviruses infecting madagascar periwinkle (catharanthus roseus) from pakistan. | though catharanthus roseus (madagascar periwinkle) is an ornamental plant, it is famous for its medicinal value. its alkaloids are known for anti-cancerous properties, and this plant is studied mainly for its alkaloids. here, this plant has been studied for its viral diseases. complete dna sequences of two begomoviruses infecting c. roseus originating from pakistan were determined. the sequence of one begomovirus (clone kn4) shows the highest level of nucleotide sequence identity (86.5 %) to an ... | 2013 | 23065111 |
| nucleotide sequence and structural analysis of two satellite rnas associated with chicory yellow mottle virus. | the two satellite rnas associated with cymv infections were sequenced. the larger (scymv-l1) has only linear molecules 1145 nucleotides long, a poly(a) tail, a long open reading frame (orf) coding for a protein of mr 39,636 resembling in composition those of other large nepovirus satellite rnas, a 5' leader sequence of 16 nucleotides and a 3' non-coding region of 40 nucleotides. in vitro translation of scymv-l1 yielded a protein product with a size that corresponded to that predicted from the se ... | 1990 | 1698918 |
| mutagenesis of a hexanucleotide sequence conserved in potexvirus rnas. | biologically active in vitro transcripts were synthesized from a cloned cdna of a defective rna (d rna) of clover yellow mosaic virus (cymv) and were used to determine if a hexanucleotide motif (5'-acuuaa) conserved in the 3' noncoding region of potexvirus rnas is essential for accumulation of progeny d rna in planta. deletion of rearrangement of the entire hexanucleotide sequence in the d rna resulted in no detectable accumulation of progeny d rna when coinoculated with helper cymv rna. single- ... | 1992 | 1641994 |
| characterization of cymbidium mosaic virus coat protein gene and its expression in transgenic tobacco plants. | cymbidium mosaic virus (cymv) is the most prevalent virus infecting orchids. here, we report the isolation of partial cdna clones encoding the genomic rna of cymv. like most of the polyadenylated monopartite positive-strand rna viruses, the open reading frame (orf) coding for the viral coat protein (cp) is located at the 3' end. the orf predicts a polypeptide chain of 220 amino acids with a molecular weight of 23,600. sequence comparison of this orf to the cp sequences of potato virus x(pvx) and ... | 1992 | 1600145 |
| coding capacity determines in vivo accumulation of a defective rna of clover yellow mosaic virus. | naturally occurring defective rnas (d rnas) derived from the potexvirus clover yellow mosaic virus (cymv) contain large internal deletions yet maintain a single open reading frame (orf) representing the in-frame fusion of 5' and 3' terminal orfs. capped transcripts of the prototype 1.2-kb d rna of cymv were synthesized in vitro and used to inoculate broad bean plants. progeny d rna accumulated only if synthetic d rna transcripts were coinoculated with cymv rna. several experiments showed that he ... | 1992 | 1560537 |
| the nucleotide sequence and genome organization of strawberry mild yellow edge-associated potexvirus. | the nucleotide sequence (5966 nucleotides) of cdna clones of strawberry mild yellow edge-associated potexvirus was determined. the genome contains six open reading frames (orfs) encoding putative proteins with mrs of 149,423, 25,344, 11,576, 8079, 25,714 and 11,216. in the first three putative proteins and the coat protein considerable similarity was found to comparable polypeptides of the potexviruses potato virus x, clover yellow mosaic virus, narcissus mosaic virus, papaya mosaic virus, white ... | 1992 | 1339469 |
| a multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees. | a multiplex polymerase chain reaction (mpcr) assay was developed to detect six rna and one dna citrus virus: citrus leaf rugose virus (clrv), citrus psorosis virus (cpsv), citrus tatter leaf virus (ctlv), citrus tristeza virus (ctv), citrus variegation virus (cvv), citrus yellow mosaic virus (cymv), and indian citrus ringspot virus (icrsv) from citrus plants. these seven viruses are classified in six different virus genera. degenerate primers were designed based on the respective virus isolate s ... | 2005 | 15951030 |
| purification of clover yellow mosaic virus. | | 1962 | 13859645 |
| sodium sulphite yields improved dna of higher stability for pcr detection of citrus yellow mosaic virus from citrus leaves. | citrus yellow mosaic virus (cymv), a non-enveloped bacilliform dna virus causes a severe mosaic disease in sweet oranges in india. cymv is weakly immunogenic, thus serodiagnosis is not a preferred method for its detection. as an alternative a rapid and reliable detection protocol by polymerase chain reaction (pcr) was developed. however, high levels of polyphenolics and tannins in citrus leaves generally interfered with obtaining good quality dna, and thus affected the reliable detection of viru ... | 2003 | 12951224 |
| inability of agrobacterium tumefaciens ribosomes to translate in vivo mrnas containing non-shine-dalgarno translational initiators. | numerous data accumulated during the last decade have shown that the shine-dalgarno (sd) sequence is not a unique initiator of translation for escherichia coli. several other sequences, mostly of viral origin, have demonstrated their capability of either enhancing or initiating translation in vivo. a phage t7 gene 10 sequence, called "epsilon" (epsilon), has shown its high enhancing activity on translation in both escherichia coli and agrobacterium tumefaciens cells. in this study the epsilon, t ... | 2002 | 12064732 |
| cloning and sequence analysis of an infectious clone of citrus yellow mosaic virus that can infect sweet orange via agrobacterium-mediated inoculation. | citrus yellow mosaic virus (cymv), a member of the family caulimoviridae, genus badnavirus, causes citrus mosaic disease, a disease that occurs commonly in india. the cymv genome has been cloned and its complete nucleotide sequence determined. its dna genome is 7559 bp in length and contains six putative open reading frames (orfs), all on the plus-strand of the genome and each capable of encoding proteins with a molecular mass of greater than 10 kda. orf 3, the largest orf, encodes a putative po ... | 2001 | 11562547 |
| cymbidium mosaic virus coat protein gene in antisense confers resistance to transgenic nicotiana occidentalis. | the nucleotide sequence of the 3'-terminal region of the korean isolate of cymbidium mosaic virus (cymv-ca) from a naturally infected cattleya was determined. the sequence contains an open reading frame (orf) coding for the viral coat protein (cp) at the 3'-end and three other orfs (triple gene block or movement protein) of cymv. the cp gene encodes a polypeptide chain of 220 amino acids with a molecular mass of 23,760 da. the deduced cp sequence showed a strong homology with those of two cymvs ... | 1999 | 10672926 |
| use of reverse transcription-polymerase chain reaction for cloning of coat protein-encoding genes of cymbidium mosaic virus. | a reverse transcription-polymerase chain reaction (rt-pcr) has been developed for the cloning of coat protein-encoding genes (cp) of cymbidium mosaic virus (cymv) isolated from three different infected species of thai orchid: cattleya, mokara and oncidium. the analysis of the compiled sequences of the cdna clones shows a single open reading frame which encoded a cymv cp gene. the gene is 669 nucleotides (nt) long and codes for a 23 761-da protein. the nt sequences of cymv cp from the thai isolat ... | 1996 | 8955635 |
| an appraisal of the banded and paracrystalline cytoplasmic inclusions induced in cymbidium mosaic potexvirus- and odontoglossum ringspot tobamovirus infected orchid cells using confocal laser scanning microscropy. | the cymbidium mosaic potexvirus (cymv) banded inclusions and the odontoglossum ringspot tobamovirus (orsv) paracrystalline inclusions were studied in flowers and leaves of nine orchid cultivars using the confocal laser scanning microscope. the inclusions varied in length and width and were mostly located adjacent to the cell walls. some orsv inclusions fully extended across entire infected cells. we propose that the cymv banded inclusion was formed from virus aggregates which aligned as periodic ... | 1996 | 8634017 |
| production of infectious in vitro transcripts from a full-length clover yellow mosaic virus cdna clone. | a full-length cdna copy of clover yellow mosaic virus (cymv) rna was constructed from two smaller cdna clones. in vitro transcription of linearized plasmid with t7 rna polymerase produced genomic-sized rna. these transcripts caused symptoms typical of cymv infection when used to inoculate both a systemic host (vicia faba) and a local lesion host (gomphrena globosa). electron microscopy of extracts from individual local lesions revealed virus particles identical to native cymv. increasing the len ... | 1993 | 8468561 |
| rapid detection of cymbidium mosaic virus by the polymerase chain reaction (pcr). | a direct, simple and sensitive method was developed for the detection of cymbidium mosaic virus (cymv), based on the polymerase chain reaction (pcr). two oligonucleotide primers were selected from regions that are homologous to potexviruses and cymv, and used to hybridize with purified viral rna and particles. this resulted in the amplification of a 313 bp fragment after 30 cycles of pcr in all samples. a less prominent fragment of 227 bp was also obtained due to mispriming of the second primer. ... | 1993 | 8432762 |
| cymbidium mosaic virus rna synthesis in isolated orchid protoplasts. | an in vitro protoplast host system has been developed to facilitate the study of cymbidium mosaic virus (cymv) replication mechanisms. cymv, a member of the potexvirus group of plant viruses, is among the most significant pathogens of cultivated orchid plants, but a convenient system for studying its molecular genetics has not been available previously. a cdna probe representing a segment of the cymv genome was used to detect viral rna following inoculation of vanda floral protoplasts with virus ... | 1993 | 8249447 |
| partial purification and characterization of foxtail mosaic potexvirus rna-dependent rna polymerase. | the rna-dependent rna polymerase (rdrp) of foxtail mosaic virus (fmv) was partially purified from infected leaves of chenopodium quinoa. the membrane fraction of crude plant extracts contained most of the fmv rdrp activity. additional purification was obtained by solubilization of the rdrp using kcl and dodecyl-sucrose and by centrifugation through a glycerol gradient. the rna template endogenous to rdrp preparations could be removed using micrococcal nuclease but the resulting fraction was unab ... | 1993 | 8249292 |
| identification and full sequence of an isolate of alternanthera mosaic potexvirus infecting phlox stolonifera. | a potexvirus was isolated from creeping phlox (phlox stolonifera) plants from a commercial nursery in pennsylvania. the virus was serologically related to clover yellow mosaic virus, plantain virus x, potato virus x, and potato aucuba mosaic virus, and was most closely related to papaya mosaic virus (papmv). the sequence of a pcr fragment obtained with potexvirus group-specific primers was distinct from that of papmv; the coat protein (cp) gene and 3' untranslated region (utr) were closely relat ... | 2006 | 16211329 |
| detection and serological relationships of cymbidium mosaic potexvirus isolates. | twenty-two isolates of cymbidium mosaic virus (cymv) were isolated from 35 orchid plants suspected of being infected with cymv. among the three methods used for detecting cymv, immunoelectron microscopy (iem-1) was shown to be the most sensitive method, being able to detect the virus in 71.43% of suspected cymv-infected plants while the electron microscopic method and the indexing plant method could detect 51.43 and 42.86%, respectively. out of 12 symptomless plants investigated, 25% were found ... | 1999 | 16232444 |
| the assembly of clover yellow mosaic virus and its protein. | clover yellow mosaic virus has been reconstituted near neutrality at low ionic strength. reconstitution is stopped by low temperatures or by 0.1 m kcl or 10(-3) m mgcl2. assembly is not confined to homologous rna. protein assembles into stacked-ring tubes near ph 8.0 or helical structures of two diameters at ph 5.0 to 5.5. | 1979 | 18631613 |
| translational strategies in potexviruses: products encoded by clover yellow mosaic virus, foxtail mosaic virus, and viola mottle virus rnas in vitro. | we have examined the template properties of the genomic rnas from three potexviruses: clover yellow mosaic virus (cymv), foxtail mosaic virus (fmv), and viola mottle virus (vmv). all three rnas encode a large (160,000 to 182,000 molecular weight) nonstructural protein when translated in the rabbit reticulocyte cell-free system. only cymv rna is able to direct the synthesis of significant quantities of a product whose electrophoretic mobility, antigenic determinants, and partial peptide map resem ... | 1986 | 18640646 |
| molecular cloning of clover yellow mosaic virus rna: identification of coat protein coding sequences in vivo and in vitro. | we have prepared double-stranded cdna from clover yellow mosaic virus (cymv) rna and have created a library of cloned cymv cdnas in plasmid vectors. cloned fragments ranging from 0.2 to 2.0 kbp have been mapped relative to the cymv genome and to each other by digestion with restriction enzymes and by colony hybridization. together, these cloned dnas constitute almost 90% of the cymv genome. two overlapping plasmids whose inserts originate from the 3' portion of cymv rna arrest the synthesis of c ... | 1987 | 18644558 |
| Sequencing of the Tamus red mosaic virus genome: further evidence that it is a distinct species of the genus Potexvirus. | In 1993, a virus causing red mosaic and leaf distortion has been isolated from black bryony (Tamus communis) in Italy. Based on particle properties and serology, the virus was assigned to the genus Potexvirus and named Tamus red mosaic virus (TRMV), pending a genome sequence. The original Italian TRMV isolate was submitted to the DSMZ plant virus collection (PV-0397). To confirm the taxonomic status of the virus, the entire genome sequence was determined comprising 6,495 nucleotides excluding th ... | 2011 | 21881939 |
| development of multiplex polymerase chain reaction assay for simultaneous detection of clostero-, badna- and mandari-viruses along with huanglongbing bacterium in citrus trees. | citrus trees harbor a large number of viral and bacterial pathogens. citrus yellow vein clearing virus (cyvcv), indian citrus ringspot virus (icrsv), citrus yellow mosaic virus (cymv), citrus tristeza virus (ctv) and a bacterium, candidatus liberibacter asiaticus (cla) associated with huanglongbing (hlb) disease, the most prevalent pathogens in citrus orchards of different regions in india and are responsible for debilitating citriculture. for detection of these viral and bacterial pathogens a q ... | 2016 | 27208471 |
| the generality of cation-binding sites in rod-shaped viruses. | hydrogen-ion titration curves have been measured for two filamentous plant viruses (clover yellow mosaic virus and potato virus x) and two filamentous bacterial viruses (fd and pf1) with and without ca2+ or mg2+ ions present, and for the protein of the pm6 mutant of tobacco mosaic virus. the bacterial viruses do not possess the 'strong' cation-binding sites found in all plant viruses, but they have 'weak' sites that can be assigned to juxtaposed carboxylate groups on their external surfaces. the ... | 1981 | 7295903 |