| expression of marek's disease virus in producer and non-producer lymphoblastoid cell lines. | continuous in vitro propagation of a producer marek's disease (md) lymphoblastoid cell line reduced the number of cells producing virus antigens and the inducibility of these antigens to 5-iododeoxyuridine (iudr) but did not change the oncogenicity of the cells for chickens. in contrast, propagation of a non-producer md cell line did not change the non-productivity of the cell line, but decreased the transplantability of the cells. the md lines were free of replicating avian leukosis sarcoma vir ... | 1978 | 221388 |
| occurrence of chloroplast ribosome recognition sites within conserved elements of the rna genomes of carlaviruses. | the nucleotide sequences upstream from the carlavirus open reading frames were examined for direct sequence homology. blocks of homology were evident upstream from the 25 k orfs of potato virus s (pvs), potato virus m (pvm) and lily symptomless virus (lsv), and upstream from the coat protein initiation codons of pvs, pvm, lsv, carnation latent virus and helenium virus s. these blocks, which correspond to the 5'-terminal regions of the subgenomic rnas, were shown to contain potential ribosome rec ... | 1991 | 2013334 |
| homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus x) from lily plants. | cdna clones complementary to the 3'-terminal regions of the genomic rnas of the carlavirus lily symptomless virus and the potexvirus lily virus x (lvx) have been sequenced. the carlavirus rna sequence contains five open reading frames (orfs) coding for proteins of mr 25,374, 11,631, 6960, 32,041 (coat protein) and 16,121, which are all very similar in size, amino acid sequence and relative position in the genome to proteins encoded by two different potato carlaviruses. the first four of these pr ... | 1990 | 2129538 |
| synthesis of hepatitis b surface antigen in mammalian cells: expression of the entire gene and the coding region. | we have constructed two simian virus 40 early replacement recombinants that have the coding sequences for hepatitis b virus surface antigen (hbsag). one construction, lsv-hbsag, has the coding region for hbsag but not the portion encoding the putative pre-surface antigen leader. transformed monkey kidney cells (cos) infected with this recombinant express large quantities of the characteristic partially glycosylated hbsag molecule, which are assembled into 22-nm particles that appear similar to t ... | 1983 | 6310147 |
| effect of ph and other conditions on immunosorbent electron microscopy of several plant viruses. | the efficiency of trapping, using immunosorbent electron microscopy, of particles of cucumber mosaic virus, lily symptomless virus, potato virus y, and carnation mottle virus, depended markedly on the ph of the virus extract. varying the ph of the serum had a smaller, though still significant effect. with potato virus y, the optimal ph of the virus extract varied with the host plant. it appears that for each virus and host a specific testing procedure is required to find the optimal trapping con ... | 1982 | 6752161 |
| nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. | we have synthesized and mapped a library of cdna clones representing the rna genome of a strain of blueberry scorch carlavirus (bbscv) associated with a disease known locally, in new jersey, u.s.a., as sheep pen hill disease. the nucleotide sequence of that strain was determined to be 8514 residues, excluding the poly(a) tail. in addition, cdna clones representing the 3' terminus of another strain of the virus from the same field were synthesized, mapped and sequenced. the overall identity betwe ... | 1994 | 8151289 |
| establishment and characterization of cell line lsv5 that retains the altered adhesive properties of human junctional epidermolysis bullosa keratinocytes. | herlitz junctional epidermolysis bullosa (h-jeb) is characterized by hampered expression of the adhesion ligand laminin-5. thus far, analysis of the processes underlying the epithelial-mesenchymal dysadhesion marking this disease has been limited by the reduced growth and adhesive capabilities of the epithelial cells derived from h-jeb patients. to overcome these difficulties, we used sv40 virus to immortalize h-jeb keratinocytes with a homozygous nonsense mutation in the gene that encodes the g ... | 1996 | 8612705 |
| phylogenetic analysis of triple gene block viruses based on the tgb 1 homolog gene indicates a convergent evolution. | the complete nucleotide sequence of the triple gene block one (tgb 1) of cymbidium mosaic potexvirus (cymmv) was compared to those from other potex-, carla-, furo- and hordeiviruses. seven conserved motifs in the tgb 1, including the atp-gtp binding domain (p-loop) consensus gxxgktsts, were found in all four virus genera. we propose that all tgbv can be classified into phylogenetic clusters based on their tgb 1 homolog genes. these clusters can be further delineated to form subgroups. the first ... | 1998 | 9654683 |
| nucleotide sequence analysis of the 3'-terminal region of two korean isolates of lily symptomless carlavirus and expression of the coat protein in e. coli. | the 3'-terminal regions of the genomic rnas of two korean isolates of the lily symptomless carlavirus (lsv), lsv-ko and lsv-kii, were cloned and their nucleotide sequences were determined. the nucleotide sequence analysis and protein analysis by the western blot revealed that e. coli expressed a 32-kda protein that is the viral coat protein (cp) for the lsv. the two korean strains share 98.4% and 98.3% sequence identities at the nucleotide and amino acid levels, respectively. the cp gene of lsv- ... | 1999 | 10420996 |
| control of aphid-borne lily symptomless virus and lily mottle virus in lilium in the netherlands. | an overview is presented on the management of viruses of lilium crops in the netherlands since the 1960s. this mainly concerns lily symptomless virus (lsv) and lily mottle virus (lmov). various factors which affect the efficiency of control are considered. the variable symptoms of lmov in the many vegetatively propagated cultivars grown (c. 340) pose problems in the efficient roguing of diseased plants of some cultivars. additionally the acceptable incidence of viruses such as lsv which are gene ... | 2000 | 11137159 |
| analysis of the triple gene block and coat protein sequences of two strains of kalanchoë latent carlavirus. | a region of the kalanchoë latent carlavirus (klv) genome, containing the coding capacity for the triple gene block and the coat protein, was cloned and sequenced for two isolates, the one infecting chenopodium quinoa systemically whereas the other infects c. quinoa locally. the sequence confirmed the classification of klv as a carlavirus. there was the highest identity in the amino acid sequences to the carlaviruses potato rough dwarf virus, lily latent virus, lily symptomless virus, blueberry s ... | 2001 | 11450944 |
| the complete nucleotide sequence of the genome rna of lily symptomless virus and its comparison with that of other carlaviruses. | the complete genomic nucleotide sequence and genome structure of lily symptomless virus (lsv), a lily-infecting carlavirus, have been obtained. the genome of the korean strain of lsv, lsv-kr, was 8394 nucleotides long and contained six open reading frames (orfs) coding for proteins of mr 220 kda (1948 aa), 25 kda (228 aa), 12 kda (106 aa), 7 kda (64 aa), 32 kda (291 aa) and 16 kda (140 aa) from the 5' to 3' end, respectively, which is typical of carlaviruses. genetic heterogeneity was observed i ... | 2003 | 14551817 |
| occurrence and sequences of lily mottle virus and lily symptomless virus in plants grown from imported bulbs in zhejiang province, china. | degenerate primers were used to amplify virus sequences from imported lilies in zhejiang province, china. two viruses, lily mottle virus (lmov, genus potyvirus) and lily symptomless virus (lsv, genus carlavirus) were detected, purified and completely sequenced from a mixed infection in a plant raised from bulbs imported from the netherlands. the sequence of lmov was 9644 nt long and encoded a polyprotein of 3095 amino acids with a calculated m(r) of 351.0 kda that had only 45.1-54.4% identity to ... | 2003 | 14648295 |
| electroanalytical determination of d(gcgaagc) hairpin. | hairpins (or hairpin-like structures) may play a major role in expansion events of triplet repeat expansion diseases (x syndrome, huntington's disease, friedreich's ataxia). the d(gcgaagc) fragment has been found in the replication origins of phage phix 174 and herpes simplex virus, in a promoter region of an escherichia coli heat-shock gene, and in rrna genes. the paper deals with the application of electrochemical methods to the determination of the dna heptamer-d(gcgaagc) which forms very sta ... | 2004 | 15110243 |
| square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal dna. | azidothymidine (azt, 3'-azido-3'-deoxythymidine, zidovudine, retrovir) is an approved and widely used antiretroviral drug for the treatment of human immunodeficiency virus (hiv) infection. dynamic electrochemical methods have been employed for the fast and inexpensive determination of this drug in natural samples. the electrochemical signal of azt, resulting from the reduction of azido group, was studied by square wave voltammetry (swv), linear sweep voltammetry (lsv) and elimination voltammetry ... | 2004 | 15110244 |
| [production of monoclonal antibodies to lily symptomless virus and application in lily virus detection]. | four hybridoma cell lines, 2a2, 5h9, 5h2 and 5e12, secreting monoclonal antibodies (mabs) against lily symptomless virus (lsv) were produced by fusing mouse myeloma cells (sp2/0) with spleen cells from balb/c immunized by the lsv particles. the four mabs could specifically react with lsv. the titres of ascitic fluids of the four mabs are up to 10(-6) in elisa. isotypes and subclasses of 5h9 and 5e12 belong to igg1 while those of 2a2 and 5h2 belong to igg3. isotypes of light strains of the four m ... | 2005 | 16245875 |
| the complete nucleotide sequence of passiflora latent virus and its phylogenetic relationship to other carlaviruses. | a virus identified as passiflora latent virus (plv) was isolated from passion fruit plants. particle morphology, host range and serological properties suggested that this virus belongs to the genus carlavirus. the complete genomic sequence of plv was determined by sequencing overlapping cdna fragments. the genome consisted of 8386 nt, excluding the poly (a) tail and contained six open reading frames, typical of carlaviruses. the overall similarities of the predicted amino acid sequence of plv to ... | 2007 | 16932981 |
| comparison of two gel filtration chromatographic methods for the purification of lily symptomless virus. | lily symptomless virus (lsv) occurs frequently in many lilium species worldwide and often causes developmental abnormalities such as a smaller flower and lower bulb yield. in this study, two moderate and efficient gel filtration chromatography (gfc) methods was compared, these two techniques were, respectively, based on superdex-200 hr and sephacryl s-1000 sf. the products purified by the two methods were then characterized by measurements with uv-spectrophotometer, reverse transcriptase (rt)-pc ... | 2007 | 17207540 |
| recombination and phylogeographical analysis of lily symptomless virus. | the complete genomic nucleotide sequence of an indian isolate of lily symptomless virus (lsv) was determined by sequencing 11 overlapping cdna fragments of different sizes. the genome consisted of 8,394 nucleotides, excluding the poly (a) tail and contained six open reading frames (orfs) coding protein for orf1 220 kda [1,948 amino acid (aa)], orf2 25 kda (228 aa), orf3 12 kda (106 aa), orf4 7 kda (64 aa), orf5 32 kda (291 aa) and orf6 16 kda (140 aa) from 5' to 3' end. sequence was analyzed wit ... | 2008 | 18202906 |
| a simple, sensitive, specific detection of mixed infection of multiple plant viruses using macroarray and microtube hybridization. | a simple, sensitive and specific method using a cdna macroarray to detect multiple viruses was devised. the method is used in plants such as potato and lily, which need a reliable routine diagnosis for mixed infection. the biotinylated crna targets were prepared using an in vitro transcription-based system that was designed especially to eliminate nonspecific hybridizations. the macroarray hybridization was carried out using a convenient, cost-effective "microtube hybridization" (mth) system. by ... | 2008 | 18760308 |
| electrochemical studies of the interaction of basic brown g with dna and determination of dna. | a new method of electrochemical probe has been proposed for the determination of herring sperm dna (dna) based on its interaction with basic brown g (bbg). the electrochemical behavior of interaction of bbg with dna was investigated on hg electrode. in 0.1moll(-1) nh(3)-nh(4)cl buffer solution (ph 8.0), bbg can be reduced on hg electrode with a well-defined voltammetric peak at -0.67v (versus sce). in the presence of dna, the reduction peak current of bbg decreases and the peak potential shifts ... | 2007 | 19071277 |
| is routine torch screening warranted in neonates with lenticulostriate vasculopathy? | congenital infections are associated with a wide spectrum of clinical symptoms, including lenticulostriate vasculopathy (lsv). | 2010 | 19887856 |
| expression, purification and characterization of the lily symptomless virus coat protein from lanzhou isolate. | lily symptomless virus (lsv) is widespread in many countries where lily are grown or planted, and causes severe economic losses in terms of quantity and quality of flower and bulb production. to study the structure-function relationship of coat protein (cp) of lsv, to investigate antigenic relationships between coat protein subunits or intact virons, and to prepare specific antibodies against lsv, substantial amounts of cp protein are needed. | 2010 | 20144245 |
| sequence and structure prediction of rna-dependent rna polymerase of lily symptomless virus isolated from l. × 'casablanca'. | the dna sequence of the rna-dependent rna polymerase (rdrp) gene of lily symptomless virus (lsv), a lily-infecting member of the genus carlavirus, was determined from nine overlapping cdna fragments of different sizes. the complete sequence of this rdrp gene (hm070294) consisted of 5,847 nucleotides coding for a protein of 220 kda. it had 97-98% sequence identity with rdrps of other known isolates at both the dna and the amino acid level. phylogenetic analysis indicated that this rdrp (designate ... | 2011 | 21409447 |
| rapid detection of lily symptomless virus with cdte quantum dots by flow cytometry. | lily symptomless virus (lsv) is the most common lily virus, being detected in many species and hybrids. we established a microsphere-based fluorescent immunoassay for the determination of lsv, using a polyclonal antibody against lsv covalently bound to carboxy-modified microspheres able to capture lsv antigen. a monoclonal antibody against lsv conjugated to quantum dots (qds) was used as a fluorescent probe, enabling lsv to be fluorescently detected by a combination of encoded beads and qds. thi ... | 2011 | 21728819 |
| mirabilis jalapa mottle virus: a new carlavirus infecting four o'clocks. | analysis of a next-generation sequence dataset from mirabilis jalapa resulted in the discovery of a novel virus in the genus carlavirus (family betaflexiviridae), mirabilis jalapa mottle virus (mjmv). the complete genome of mjmv was determined to consist of 8315 nucleotides (nt), with the six open reading frames indicative of carlaviruses. mjmv is most similar to kalanchoe latent virus (60% identity) and lily symptomless virus (59% identity). the virus can be transmitted mechanically to mirabili ... | 2011 | 21915718 |
| the genome sequence of lone star virus, a highly divergent bunyavirus found in the amblyomma americanum tick. | viruses in the family bunyaviridae infect a wide range of plant, insect, and animal hosts. tick-borne bunyaviruses in the phlebovirus genus, including severe fever with thrombocytopenia syndrome virus (sftsv) in china, heartland virus (hrtv) in the united states, and bhanja virus in eurasia and africa have been associated with acute febrile illness in humans. here we sought to characterize the growth characteristics and genome of lone star virus (lsv), an unclassified bunyavirus originally isola ... | 2013 | 23637969 |
| comprehensive bee pathogen screening in belgium reveals crithidia mellificae as a new contributory factor to winter mortality. | since the last decade, unusually high honey bee colony losses have been reported mainly in north-america and europe. here, we report on a comprehensive bee pathogen screening in belgium covering 363 bee colonies that were screened for 18 known disease-causing pathogens and correlate their incidence in summer with subsequent winter mortality. our analyses demonstrate that, in addition to varroa destructor, the presence of the trypanosomatid parasite crithidia mellificae and the microsporidian par ... | 2013 | 23991113 |
| extraction of total nucleic acid based on silica-coated magnetic particles for rt-qpcr detection of plant rna virus/viroid. | in this study, a nucleic acid extraction method based on silica-coated magnetic particles (smps) and rt-qpcr assay was developed to detect arabis mosaic virus (armv), lily symptomless virus (lsv), hop stunt viroid (hsvd) and grape yellow speckle viroid 1 (gysvd-1). the amplification sequences of rt-qpcr were reversely transcribed in vitro as rna standard templates. the standard curves covered six or seven orders of magnitude with a detection limit of 100 copies per each assay. extraction efficie ... | 2014 | 24291163 |
| honey bee infecting lake sinai viruses. | honey bees are critical pollinators of important agricultural crops. recently, high annual losses of honey bee colonies have prompted further investigation of honey bee infecting viruses. to better characterize the recently discovered and very prevalent lake sinai virus (lsv) group, we sequenced currently circulating lsvs, performed phylogenetic analysis, and obtained images of lsv2. sequence analysis resulted in extension of the lsv1 and lsv2 genomes, the first detection of lsv4 in the us, and ... | 2015 | 26110586 |
| reverse transcription polymerase chain reaction-based system for simultaneous detection of multiple lily-infecting viruses. | a detection system based on a multiplex reverse transcription (rt) polymerase chain reaction (pcr) was developed to simultaneously identify multiple viruses in the lily plant. the most common viruses infecting lily plants are the cucumber mosaic virus (cmv), lily mottle virus (lmov), lily symptomless virus (lsv). leaf samples were collected at lily-cultivation facilities located in the kangwon province of korea and used to evaluate the detection system. simplex and multiplex rt-pcr were performe ... | 2013 | 25288961 |
| development of an indirect elisa and immunocapture rt-pcr for lily virus detection. | multiple viruses such as lily symptomless virus (lsv), lily mottle virus (lmov), and cucumber mosaic virus (cmv) are the most prevalent viruses infecting lilies in korea. leaf samples and bulbs showing characteristic symptoms of virus infection were collected from gangwon, chungnam, and jeju provinces of korea in 2008-2011. coat protein (cp) genes of lsv and lmov were amplified from collected samples by reverse transcription-polymerase chain reaction (rt-pcr) and cloned into a pet21d(+) expressi ... | 2012 | 23221542 |
| amperometric sensing of anti-hiv drug zidovudine on ag nanofilm-multiwalled carbon nanotubes modified glassy carbon electrode. | the zidovudine (zdv) is the first drug approved for the treatment of hiv virus infection. the detection and determination of this drug are very importance in human serum because of its undesirable effects. a new zdv sensor was fabricated on the basis of nanocomposite of silver nanofilm (ag-nf) and multiwalled carbon nanotubes (mwcnts) immobilized on glassy carbon electrode (gce). the modified electrodes were characterized by scanning electron microscopy (sem), energy dispersive x-ray spectroscop ... | 2014 | 24863205 |
| rapid and sensitive detection of lily symptomless virus by reverse transcription loop-mediated isothermal amplification. | lily symptomless virus (lsv) is one of the most prevalent viruses that infect lily plants worldwide. a rapid and sensitive reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detection of lsv, using two primer pairs that specifically amplified the conserved sequence of lsv coat protein. the optimum reaction conditions were as follows: 4mm mgcl2 and 0.8m betaine with incubation at 64°c for 30min. the limit of detection of lsv from infected lily leaves wa ... | 2016 | 27737784 |
| varroa destructor macula-like virus, lake sinai virus and other new rna viruses in wild bumblebee hosts (bombus pascuorum, bombus lapidarius and bombus pratorum). | pollinators such as bumblebees (bombus spp.) are in decline worldwide which poses a threat not only for ecosystem biodiversity but also to human crop production services. one main cause of pollinator decline may be the infection and transmission of diseases including rna viruses. recently, new viruses have been discovered in honeybees, but information on the presence of these in wild bumblebees is largely not available. in this study, we investigated the prevalence of new rna viruses in bombus s ... | 2016 | 26706994 |
| the etiology of lenticulostriate vasculopathy and the role of congenital infections. | lenticulostriate vasculopathy (lsv) refers to increased echogenicity of the penetrating vessels that supply the basal ganglia and segments of the internal capsule seen on cranial ultrasound. initially identified in infants with congenital infection, lsv has now been associated with a variety of infectious and non-infectious conditions. although robust epidemiologic studies are lacking, the available evidence does not support broad evaluation for multiple congenital infections when lsv is identif ... | 2015 | 25960415 |
| development of an immunochromatographic strip test for rapid detection of lily symptomless virus. | a rapid immunochromatographic strip (ics) test for specific detection of lily symptomless virus (lsv) was developed. the test is based on a double-antibody sandwich format and employs two distinct anti-lsv polyclonal antibodies (igg1 and igg2). the first antibody, igg1 was used as the detection antibody conjugated to colloidal gold and the second antibody, igg2 was used to as the capture antibody at the test line. the performance of the ics test was evaluated and the results obtained were compar ... | 2015 | 25845624 |
| genome sequence heterogeneity of lake sinai virus found in honey bees and orf1/rdrp-based polymorphisms in a single host. | honey bees (apis mellifera) are susceptible to a wide range of pathogens, including a broad set of viruses. recently, next-generation sequencing has expanded the list of viruses with, for instance, two strains of lake sinai virus. soon after its discovery in the usa, lsv was also discovered in other countries and in other hosts. in the present study, we assemble four almost complete lsv genomes, and show that there is remarkable sequence heterogeneity based on the orf1, rna-dependent rna polymer ... | 2015 | 25725149 |
| metagenomic detection of viral pathogens in spanish honeybees: co-infection by aphid lethal paralysis, israel acute paralysis and lake sinai viruses. | the situation in europe concerning honeybees has in recent years become increasingly aggravated with steady decline in populations and/or catastrophic winter losses. this has largely been attributed to the occurrence of a variety of known and "unknown", emerging novel diseases. previous studies have demonstrated that colonies often can harbour more than one pathogen, making identification of etiological agents with classical methods difficult. by employing an unbiased metagenomic approach, which ... | 2013 | 23460860 |