| genome structure and complete sequence of genomic rna of daphne virus s. | the complete genomic nucleotide sequence and structure of daphne virus s (dvs), a daphne-infecting member of the genus carlavirus, were determined. the genome of dvs was 8,739 nucleotides long, excluding the poly (a) tails. the genome of dvs contained six open reading frames coding for proteins of mr 227 kda (viral replicase), 25 kda, 11 kda and 7 kda (triple gene block tgb) proteins 1, 2 and 3), 35 kda (coat protein; cp), and 12 kda from the 5' to 3' ends; respectively. this is the typical geno ... | 2006 | 16096707 |
| characterization of the 3'-terminal nucleotide sequence of two korean isolates of daphne virus s support its placement as a distinct species of the genus carlavirus. | this study determined the 3'-terminal nucleotide sequences of two korean isolates of daphne virus s (dvs), a tentative member of the genus carlavirus, causing leaf distortion and chlorotic spot disease symptoms in daphne plants. the 3'-terminal 1,465 nucleotide sequences of the two isolates contained two open reading frames coding for proteins of 36 kda viral coat protein (cp) and 12 kda from the 5'-3' end, which is a typical genome structure of the 3'-terminal region of carlaviruses. both dvs i ... | 2003 | 14551815 |
| nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. | we have synthesized and mapped a library of cdna clones representing the rna genome of a strain of blueberry scorch carlavirus (bbscv) associated with a disease known locally, in new jersey, u.s.a., as sheep pen hill disease. the nucleotide sequence of that strain was determined to be 8514 residues, excluding the poly(a) tail. in addition, cdna clones representing the 3' terminus of another strain of the virus from the same field were synthesized, mapped and sequenced. the overall identity betwe ... | 1994 | 8151289 |
| nucleotide sequence of the 7 k gene of helenium virus s. | the sequence of 380 nucleotides upstream of the start codon of the open reading frame (orf) of the coat protein of the carlavirus helenium virus (helvs) was determined from cloned cdna. this portion of the viral rna contained one complete orf encoding a protein of 7 k which had homology with similar sized proteins from other members of the carla- and potexvirus groups. sequence data was also obtained beyond the start codon of the 7 k orf into what was presumed to be the 12 k protein gene of the ... | 1993 | 8010191 |
| nucleotide sequence of the 3'-terminal region of helenium virus s rna. | the sequence of 1390 nucleotides of the 3'-terminal region of helenium virus s rna was determined from cloned cdna. this portion of the viral rna contains two major open reading frames (orfs) encoding proteins of mr 32.9k and 12.6k. the 32.9k protein, which is similar in size to the capsid protein of the virus, shares marked homology with the coat proteins of potexviruses and two other carlaviruses. the orf, corresponding to mr 12.6k, nearest to the 3'-terminal poly(a) tail shows extensive simil ... | 1990 | 2391504 |
| occurrence of chloroplast ribosome recognition sites within conserved elements of the rna genomes of carlaviruses. | the nucleotide sequences upstream from the carlavirus open reading frames were examined for direct sequence homology. blocks of homology were evident upstream from the 25 k orfs of potato virus s (pvs), potato virus m (pvm) and lily symptomless virus (lsv), and upstream from the coat protein initiation codons of pvs, pvm, lsv, carnation latent virus and helenium virus s. these blocks, which correspond to the 5'-terminal regions of the subgenomic rnas, were shown to contain potential ribosome rec ... | 1991 | 2013334 |
| expression of helenium virus s coat protein in escherichia coli, in vitro in rabbit reticulocyte lysate and transgenic tobacco. | the coat protein open reading frame (orf) sequence of helenium virus s (helvs) was cloned and expressed in e. coli, rabbit reticulocyte and transgenic tobacco. in e. coli the size of the protein was identical to that obtained for the coat protein from purified virus particles and less than that predicted for the fusion protein. this may be due to ribosome binding at a potential ribosome binding site present on the viral sequence, approximately 45 nucleotides upstream from the initiating methioni ... | 1992 | 1363991 |