| [the use of triton x-100 for purification of potato virus s]. | | 1975 | 1214645 |
| translation of potato virus s rna in vitro: evidence of protein processing. | rna from potato virus s (pvs), a member of the carlavirus group, was translated in vitro in rabbit reticulocyte lysate. time-course experiments revealed the largest product of mr 190 kd, decreasing in intensity after 60-min incubations, correlating with the accumulation of a 150-kd peptide. this apparent processing could be blocked by the addition of the amino-acid analogues p-fluorophenylalanine and l-canavanine for phenylalanine and arginine, respectively. l-canavanine also appeared to specifi ... | 1992 | 1312755 |
| analysis of the coat proteins of the ordinary and andean strains of potato virus s and antigenic comparisons of carlaviruses. | the coat proteins of both ordinary (pvso) and andean (pvsa) strains were shown to be similar in molecular weights, peptide maps, amino acid analysis and reaction to a range of carlavirus antiseras. | 1992 | 1359770 |
| the 3'-nucleotide sequence of an ordinary strain of potato virus s. | the sequence of 1346 nucleotides of the 3'-terminal region of an ordinary strain of potato virus s was determined from cloned cdna. this portion of the viral rna contains two major open reading frames (orfs), encoding the coat protein of m(r)33k and a protein of m(r)11k 3' to the coat protein, both of which showed high homology, 93% and 79%, respectively, with the equivalent proteins of the andean strain. a major block of amino acids that differ between the ordinary and andean strains was presen ... | 1992 | 1413539 |
| evidence for subgenomic rnas in leaves infected with an andean strain of potato virus s. | the genomic rna (7.5 kb) and two subgenomic rna species (2.5 kb and 1.1 kb) were detected by northern hybridization in leaves infected with an andean strain of potato virus s (pvsa). the single-stranded rnas contain a substantial poly-(a) tract as shown by oligo (dt)-cellulose chromatography and by an increased mobility on agarose gels following digestion with rnase h in the presence of oligo (dt). in rabbit reticulocyte lysates, the 1.1 kb rna directed the synthesis of the 34 kd virus coat prot ... | 1991 | 1683132 |
| molecular variation between ordinary and andean strains of potato virus s. | | 1991 | 1771295 |
| in vitro membrane binding of the translation products of the carlavirus 7-kda protein genes. | two double-stranded dna copies of the genes potentially coding for the 7-kda proteins of potato virus m (pvm) and potato virus s (pvs) were synthesized and cloned into t7 transcription vectors. cell-free translation of the corresponding monocistronic transcripts yielded in both cases a single protein of approximately 7-8 kda that contains a highly hydrophobic n-terminal segment. to analyze their membrane-binding potential, both proteins were synthesized in the membrane-enriched krebs-2 extract. ... | 1991 | 1853576 |
| occurrence of chloroplast ribosome recognition sites within conserved elements of the rna genomes of carlaviruses. | the nucleotide sequences upstream from the carlavirus open reading frames were examined for direct sequence homology. blocks of homology were evident upstream from the 25 k orfs of potato virus s (pvs), potato virus m (pvm) and lily symptomless virus (lsv), and upstream from the coat protein initiation codons of pvs, pvm, lsv, carnation latent virus and helenium virus s. these blocks, which correspond to the 5'-terminal regions of the subgenomic rnas, were shown to contain potential ribosome rec ... | 1991 | 2013334 |
| transgenic nicotiana debneyii expressing viral coat protein are resistant to potato virus s infection. | the coat protein gene from potato virus s (pvs) was introduced into nicotiana debneyii by leaf disc transformation using agrobacterium tumefaciens. transgenic plants expressing the viral coat protein were highly resistant to subsequent infection by the me strain of pvs as indicated by an absence of symptom development and a lack of accumulation of virus in both the inoculated and upper leaves. as in reported experiments with plants expressing potato virus x coat protein, plants expressing pvs co ... | 1990 | 2212996 |
| a comparison of the nucleotide sequence homologies between isolates of the andean and ordinary strains of potato virus s and their relationship to other carlaviruses. | sequence homologies between the rnas of five isolates of the ordinary strain of potato virus s (pvso), four isolates of the andean strain (pvsa), potato virus m(pvm), and the type member of the carlavirus group, carnation latent virus (clv), were compared using cdna solution hybridization. a high degree of homology (90-100%) was detected between the majority of pvso and pvsa isolates, but lower levels of homology (less than 10%) were observed between pvs, pvm, and clv. | 1990 | 2270676 |
| investigation of the 5' terminal structures of genomic and subgenomic rnas of potato virus s. | hybrid arrest translation involving an antisense rna, generated from a cloned cdna close to the 5' region of potato virus s genomic rna, blocked the synthesis of genomic encoded products but had little effect on subgenomic rna encoded products. similarly, the synthesis of pvs genomic rna-directed peptides was inhibited by the cap analogue m7g5'ppp5'g, suggesting the presence of a cap structure at the 5' terminus whilst subgenomic rna encoded products remained unaffected, suggesting an uncapped s ... | 1990 | 2288071 |
| evidence for the role of subgenomic rnas in the production of potato virus s coat protein during in vitro translation. | purified virus particles of potato virus s (pvs) when disrupted yielded only one readily resolved band of 7.5 kb in ethidium bromide-stained agarose gels. however northern hybridization of viral rna, probed with a clone specific to the viral coat protein gene, revealed a region of subgenomic rnas of approximately 1.3 kb. sucrose gradient fractionation of subgenomic rna revealed that it coded for viral coat protein when translated in vitro in rabbit reticulocyte lysate. virus particle lengths sug ... | 1990 | 2345371 |
| nucleotide sequence of the 3'-terminal region of helenium virus s rna. | the sequence of 1390 nucleotides of the 3'-terminal region of helenium virus s rna was determined from cloned cdna. this portion of the viral rna contains two major open reading frames (orfs) encoding proteins of mr 32.9k and 12.6k. the 32.9k protein, which is similar in size to the capsid protein of the virus, shares marked homology with the coat proteins of potexviruses and two other carlaviruses. the orf, corresponding to mr 12.6k, nearest to the 3'-terminal poly(a) tail shows extensive simil ... | 1990 | 2391504 |
| organization and interviral homologies of the 3'-terminal portion of potato virus s rna. | the sequence of 3553 nucleotides corresponding to the 3'-terminal region of potato virus s (pvs) has been determined from cloned cdna. the sequence obtained contains six open reading frames (orfs) encoding proteins of mr 10,734, mr 32,515, mr 7,222, mr 11,802, mr 25,092 and at least mr 41,052. the sequence of the 33k orf has been confirmed to be that of the viral coat protein gene. the nucleotide sequence of this orf was obtained from plasmids which were isolated by colony hybridization with a s ... | 1989 | 2732711 |
| optimum conditions for the storage of potato virus s. | the effect of storage conditions on the serological activity of two strains of potato virus s (pvs), andean and the ordinary, was studied by elisa. virus purificates, infected leaves and their homogenates, stored in lyophilized, frozen and dissolved form at various temperatures were tested. virus purificates were most stable in lyophilized form, their activity decreased after 9 months only by 20-30%. also non-purified virus was most stable as a lyophilized leaf homogenate, its activity decreased ... | 1995 | 7572470 |
| disruption of virus movement confers broad-spectrum resistance against systemic infection by plant viruses with a triple gene block. | white clover mosaic virus strain o (wclmv-o), species of the potexvirus genus, contains a set of three partially overlapping genes (the triple gene block) that encodes nonvirion proteins of 26 kda, 13 kda, and 7 kda. these proteins are necessary for cell-to-cell movement in plants but not for replication. the wclmv-o 13-kda gene was mutated (to 13*) in a region of the gene that is conserved in all viruses known to possess triple-gene-block proteins. all 10 13* transgenic lines of nicotiana benth ... | 1994 | 7937946 |
| in vivo characterisation of a translational enhancer upstream from the coat protein open reading frame of potato virus s. | the 101 nucleotide region upstream from the atg of the potato virus s (pvs) coat protein gene was isolated and the effect of this region on the translation of a downstream open reading frame analysed in vivo. translation was monitored using the reporter genes b-glucuronidase (gus) and luciferase (luc). translational enhancement was assayed transiently using dna microprojectile bombardment into both leaf and pollen tissue and also by polyethylene glycol mediated transfection of tobacco protoplast ... | 1994 | 7979985 |
| nucleotide sequence of the 7 k gene of helenium virus s. | the sequence of 380 nucleotides upstream of the start codon of the open reading frame (orf) of the coat protein of the carlavirus helenium virus (helvs) was determined from cloned cdna. this portion of the viral rna contained one complete orf encoding a protein of 7 k which had homology with similar sized proteins from other members of the carla- and potexvirus groups. sequence data was also obtained beyond the start codon of the 7 k orf into what was presumed to be the 12 k protein gene of the ... | 1993 | 8010191 |
| analysis of a translational enhancer upstream from the coat protein open reading frame of potato virus s. | evidence has suggested that the subgenomic rna of the carlavirus potato virus s is an efficient message for the coat protein, even though evidence suggests it is uncapped at its 5' terminus. we have investigated the effect of the upstream region of the coat protein gene of potato virus s on the level of reporter gene expression in vitro. the region of 101 nucleotides upstream of the coat protein, designated vte (viral translational enhancer) was found to increase levels of translation in compari ... | 1994 | 8129619 |
| nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. | we have synthesized and mapped a library of cdna clones representing the rna genome of a strain of blueberry scorch carlavirus (bbscv) associated with a disease known locally, in new jersey, u.s.a., as sheep pen hill disease. the nucleotide sequence of that strain was determined to be 8514 residues, excluding the poly(a) tail. in addition, cdna clones representing the 3' terminus of another strain of the virus from the same field were synthesized, mapped and sequenced. the overall identity betwe ... | 1994 | 8151289 |
| partial antigenic characterization of potato virus s (andean strain) by monoclonal antibodies. | four mouse monoclonal antibodies (moabs)) against potato virus s andean strain (pvsa) were tested. while moabs 2 and 3 reacted only with complete virions and were apparently specific for epitopes dependent on quaternary structure, moabs 1 and 4 appared to be conformation independent and reacted with exposed regions on native virions as well as on the surface of dissociated coat protein subunits. this seems to be an evidence of metatope existence. the results of competitive binding tests together ... | 1996 | 8886094 |
| deletion analysis of a translational enhancer upstream from the coat protein open reading frame of potato virus s. | we have previously demonstrated that the 101 nucleotides upstream from the atg of the potato virus s (pvs) coat protein gene has the ability to act as a translational enhancer in vitro and in vivo when fused to a range of marker genes. these 101 nucleotides, which have been designated vte (viral translational enhancer), contain a block of nucleotide homology that is conserved between members of the carlavirus group, centered around a core sequence of cctttaggtt. when deletions were generated tha ... | 1997 | 9155881 |
| analysis of a translational enhancer present within the 5'-terminal sequence of the genomic rna of potato virus s. | when present as a transcript leader the 5' untranslated sequence from the potato virus s genomic rna molecule enhances translation of a downstream open reading frame both in vitro and in vivo. translational enhancement was 30-fold in rabbit reticulocyte lysate and 15 fold in wheat germ above translation from a transcript with a synthetic leader. transient expression experiments using tobacco protoplasts and particle bombardment of leaf tissue resulted in enhancement of fourteen and five-fold, re ... | 1999 | 10481751 |
| a novel usage of random primers for multiplex rt-pcr detection of virus and viroid in aphids, leaves, and tubers. | a multiplex reverse transcription polymerase chain reaction (m-rt-pcr) was developed for the simultaneous detection of five potato viruses and a viroid. the synthesis of cdnas used for amplification was primed by hexanucleotides (random primers, rp). an rna extraction procedure employing dnase i, is routinely used to isolate potato viruses and viroid (potato virus s, pvs; potato leafroll virus, plrv; potato virus x, pvx; potato virus a and y, pva, pvy; and potato spindle tuber viroid, pstvd) fro ... | 2001 | 11164484 |
| analysis of the triple gene block and coat protein sequences of two strains of kalanchoë latent carlavirus. | a region of the kalanchoë latent carlavirus (klv) genome, containing the coding capacity for the triple gene block and the coat protein, was cloned and sequenced for two isolates, the one infecting chenopodium quinoa systemically whereas the other infects c. quinoa locally. the sequence confirmed the classification of klv as a carlavirus. there was the highest identity in the amino acid sequences to the carlaviruses potato rough dwarf virus, lily latent virus, lily symptomless virus, blueberry s ... | 2001 | 11450944 |
| plant 7sl rna and trna(tyr) genes with inserted antisense sequences are efficiently expressed in an in vitro transcription system from nicotiana tabacum cells. | rna polymerase iii-driven cassettes for the expression of antisense rnas and ribozymes have recently attracted much attention because (1) pol iii genes are transcribed abundantly in all kinds of tissues and (2) the transcripts are very stable by virtue of their small and compact size. we have designed two types of pol iii-based expression vehicles. antisense rna sequences targeted against conserved structural elements or domains in the rnas of potato spindle tuber viroid, hop latent viroid and p ... | 2002 | 12374302 |
| the potato virus s resistance gene ns maps to potato chromosome viii. | the dominant allele ns confers in potato resistance to potato virus s (pvs). to identify the chromosomal location of ns, we mapped the ns-linked marker scg17(448) and the issr marker ubc811(600) to linkage group viii of the rflp map of a population that did not segregate for ns. the map position of the ns locus on chromosome viii was confirmed with the detection of linkage between ns and three rflp markers, gp126, gp189 and cp16, known to be located in a corresponding region on potato chromosome ... | 2002 | 12582505 |
| dna microarray: parallel detection of potato viruses. | dna microarray assay has become a useful tool for gene expression studies. less frequent is its application to detection of viruses or diagnostics of virus diseases. here we show design of a microscope slide-based microarray assay for simultaneous identification of several potato viruses. different primer pairs were designed or adopted to obtain specific amplicons from six potato viruses: potato virus a (pva), potato virus s (pvs), potato virus x (pvx), potato virus y (pvy), potato mop-top virus ... | 2003 | 12828343 |
| serological relations between potato paracrinkle virus, potato virus s and carnation latent virus. | | 1956 | 13385442 |
| inter-simple sequence repeat (issr) markers for the ns resistance gene in potato (solanum tuberosum l.). | inter-simple sequence repeat (issr) polymorphism was used for finding markers linked to the ns gene, responsible for a resistance of potato (solanum tuberosum l.) to potato virus s (pvs). the issr markers ubc811(660) and ubc811(950) were found to be linked to ns. linkage distances were estimated to be 2.6 cm and 6.6 cm, respectively. ubc811(660) showed high accuracy for detection of pvs resistance in diploid potato clones. in tetraploids, among seventeen studied genotypes containing the resistan ... | 2001 | 14564048 |
| evaluation of a simple membrane-based nucleic acid preparation protocol for rt-pcr detection of potato viruses from aphid and plant tissues. | a rapid and simple protocol for preparing viral rna from aphids (myzus persicae) and potato (solanum tuberosum l.) tissue (leaves, sprouts, and tubers) for reverse transcription-polymerase chain reaction (rt-pcr) was developed. the four-step method involves: (1) preparing plant crude sap or aphid macerates in a buffered detergent (triton xl-80n) solution; (2) immobilizing clarified sap on a nitrocellulose membrane; (3) performing reverse transcription using eluted water extract from cut-out spot ... | 2004 | 15381353 |
| complete nucleotide sequence and molecular probing of potato virus s genome. | complete genomes of three isolates of potato virus s (pvs) were cloned and sequenced. the pvs orf-1 was characterized for the first time. it encodes a putative replication protein (rpt) that shares the highest homology (about 52%) with that of blueberry scorch virus (blscv). orf-1 motifs, characteristic for carlaviruses were found for methyltransferase (mtr), helicase (hel) and rna-dependent rna polymerase (rdrp). the complete sequence of pvs genome enabled to develop an immunocapture rt-pcr pro ... | 2005 | 16178517 |
| occurrence and physico-serological properties of potato virus s (pvs) in iran. | 1818 collected samples of potato plant showing virus infection symptoms from 85 fields were tested for pvs infection using das-elisa. average of infection to this virus varied from 0 to 100%. least infection was belonging to fields with new introduced varieties. on the other hand native and old introduced cultivars showed heavy infection. in field condition, pvs infected plants didn't show very obvious symptoms, so some infected plants may be missed in field sample collecting. the physical prope ... | 2005 | 16637209 |
| development and evaluation of a multiplex rt-pcr for detecting main viruses and a viroid of potato. | a multiplex rt-pcr (mrt-pcr) for detecting four potato viruses (potato virus s (pvs), potato virus x (pvx), potato virus y (pvy), and potato leaf roll virus (plrv)) and one potato viroid (potato spindle tuber viroid (pstvd)) was developed. the mrt-pcr consisted of one reaction with specific primers designed according to the sequences of coat protein (cp) genes of respective viruses and the sequence of the viroid. the entire procedure from tissue grinding to rt-pcr results takes about 4 hrs. the ... | 2006 | 16808331 |
| endonuclease restriction of scar amplicons sc811 is required to identify ns-false-positive markers in pvs-susceptible potato cultivars. | the ns gene confers resistance of potato to potato virus s (pvs). sixteen german and dutch potato cultivars, all registered in poland, were found to be susceptible to pvs infection. however, scoring of the cultivars for the presence of the ns-linked scar marker sc811(454) revealed additional amplicons with a similar electrophoretic migration rate as that of sc811(454), which resulted in ambiguous determination of the genotype at the ns locus. mboi or foki treatment of the pcr products allowed to ... | 2008 | 18263969 |
| development of universal primers for detection of potato carlaviruses by rt-pcr. | to facilitate efficient and accurate detection of potato-infecting carlaviruses, degenerated universal primers were designed based on conserved amino acid and nucleotide sequences. two sense primers, car-f1 and car-f2, were based on the amino acid sequences "snnma" and "glgvpte", respectively, in the coat protein. the reverse primer, car-r, which was located at the border of the nucleic acid binding protein gene and the 3' untranslated region, and dt-b, which was derived from the oligo-dt target ... | 2008 | 18353450 |
| a single tube, quantitative real-time rt-pcr assay that detects four potato viruses simultaneously. | a high throughput, real-time multiplex, single tube rt-pcr assay was developed for simultaneous detection of potato leafroll virus (plrv), potato virus x (pvx) and potato virus s (pvs) in potato leaves and tubers, and tomato spotted wilt virus (tswv) in potato tubers and tomato leaves. the test uses four different fluorescently labelled taqman probes. limits of detection sensitivity were established using a range of virus transcript copy numbers (8 x 10(1) to 8 x 10(9) copies of pvx and pvs, 1 x ... | 2009 | 19596379 |
| complementation analysis of triple gene block of potato virus s (pvs) revealed its capability to support systemic infection and aphid transmissibility of recombinant potato virus x. | triple gene block (tgb) sequences derived from isolates of ordinary potato virus s (pvs-o) and chenopodium-systemic (pvs-cs) were analyzed. although the tgb sequences did not reveal any specific difference within the 7k protein, some specific differences within the 25k and 12k orfs were found. in order to investigate a possible functional divergence of pvs-o and pvs-cs tgb variants, these genes were propagated in chimeric potato virus x (pvx). both pvs tgb variants partly complemented pvx tgb in ... | 2009 | 19748533 |
| molecular characterization of two potato virus s isolates from late-blight-resistant genotypes of potato (solanum tuberosum). | | 2009 | 19820893 |
| genetic variability in the coat protein gene of potato virus s isolates and distinguishing its biologically distinct strains. | the complete coat protein (cp) nucleotide sequences of 13 potato virus s (pvs) isolates from australia and three from europe were compared to those of 37 others. on phylogenetic analysis, the australian sequences were in pvs(o) sub-clades iii and iv, and the european isolates were in sub-clades i and vii. the european isolates invaded chenopodium spp. systemically, but eight australian isolates did not. amino acid sequence differences at the n-terminal ends of the cps were unrelated to the abili ... | 2010 | 20467765 |
| analysis of iranian potato virus s isolates. | two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven iranian provinces. forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (das-elisa) using a potato virus s (pvs) polyclonal antibody. of these 12 isolates of pvs were selected based on the geographical location for biological and molecular characterization. the full coat protein (cp) a ... | 2011 | 21567245 |
| a new virus discovered by immunocapture of double-stranded rna, a rapid method for virus enrichment in metagenomic studies. | next-generation sequencing technologies enable the rapid identification of viral infection of diseased organisms. however, despite a consistent decrease in sequencing costs, it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. as the majority of plant viruses have an rna genome, a common approach is to extract the double-stranded rna (dsrna) replicative form, to enrich the replicating virus genetic material over the host background. the tradi ... | 2016 | 26990372 |
| multiarray on a test strip (mats): rapid multiplex immunodetection of priority potato pathogens. | multiarray on a test strip (mats) was developed for the detection of eight important potato pathogens. the proposed assay combines the rapidity of immunochromatography with the high throughput of array techniques. the test zone of the immunochromatographic strip comprises ordered rows of spots containing antibodies specific for different potato pathogens. the assay benefits from the simplicity of immunochromatography; colored immune complexes form at the corresponding spots within the test zone. ... | 2016 | 27007732 |
| simultaneous detection and identification of four viruses infecting pepino by multiplex rt-pcr. | potato virus m (pvm), pepino mosaic virus (pepmv), tomato mosaic virus (tomv), and potato virus s (pvs) infect pepino and cause serious crop losses. in this study, a multiplex rt-pcr method was developed for simultaneous detection and differentiation of pvs, tomv, pepmv and pvm. the method was highly reliable and sensitive; validation was accomplished by testing pepino samples collected from different regions of china. in this survey, pvm, tomv and pvs were detected in 37.0 %, 31.0 % and 5.5 % o ... | 2013 | 23338706 |
| discovery and characterization of a novel carlavirus infecting potatoes in china. | a new carlavirus, tentatively named potato virus h (pvh), was found on potato plants with mild symptoms in hohhot, inner mongolia autonomous region, china. pvh was confirmed by genome sequencing, serological reactions, electron microscopy, and host index assays. the pvh particles were filamentous and slightly curved, with a modal length of 570 nm. complete rna genomic sequences of two isolates of pvh were determined using reverse transcription-pcr (rt-pcr) and the 5' rapid amplification of cdna ... | 2013 | 23805334 |
| detection of multiple potato viruses in the field suggests synergistic interactions among potato viruses in pakistan. | viral diseases have been a major limiting factor threating sustainable potato (solanum tuberosum l.) production in pakistan. surveys were conducted to serologically quantify the incidence of rna viruses infecting potato; potato virus x (pvx), potato virus y (pvy), potato virus s (pvs), potato virus a (pva), potato virus m (pvm) and potato leaf roll virus (plrv) in two major potato cultivars (desiree and cardinal). the results suggest the prevalence of multiple viruses in all surveyed areas with ... | 2014 | 25506305 |
| [detection and molecular characterization of potato virus s (pvs, carlavirus) from colombia]. | in colombia, potato crops are affected by a wide variety of viruses such as pvy, plrv, pvx, pmtv and pvs. unfortunately, there are very few studies on the biology, distribution and pathogenicity of these viruses; this situation is even worse for the latent virus pvs. in this work, we evaluated the presence ofpvs in four colombian provinces (antioquia, boyacá, cundinamarca, nariño) by the use of elisa. we also studied the degree of molecular variation by sequence comparison of a segment of the ge ... | 2013 | 23885575 |
| viruses of potato. | potatoes are an important crop in mediterranean countries both for local consumption and for export to other countries, mainly during the winter. many mediterranean countries import certified seed potato in addition to their own seed production. the local seeds are mainly used for planting in the autumn and winter, while the imported seed are used for early and late spring plantings. potato virus y is the most important virus in mediterranean countries, present mainly in the autumn plantings. th ... | 2012 | 22682169 |
| rnai-mediated simultaneous resistance against three rna viruses in potato. | rna interference (rnai) technology has been successfully applied in stacking resistance against viruses in numerous crop plants. during rnai, the production of small interfering rnas (sirnas) from template double-standard rna (dsrna) derived from expression constructs provides an on-switch for triggering homology-based targeting of cognate viral transcripts, hence generating a pre-programmed immunity in transgenic plants prior to virus infection. in the current study, transgenic potato lines (so ... | 2017 | 28194691 |
| molecular characterization of domestic and exotic potato virus s isolates and a global analysis of genomic sequences. | five potato virus s (pvs) isolates from the usa and three isolates from chile were characterized based on biological and molecular properties to delineate these pvs isolates into either ordinary (pvs(o)) or andean (pvs(a)) strains. five isolates - 41956, cosimar, galaxy, nd2492-2r, and q1 - were considered ordinary strains, as they induced local lesions on the inoculated leaves of chenopodium quinoa, whereas the remaining three (fl206-1d, q3, and q5) failed to induce symptoms. considerable varia ... | 2014 | 24610552 |
| complete genome sequence of a novel potato virus s strain infecting solanum phureja in colombia. | potato virus s (pvs) (genus carlavirus, family betaflexiviridae) is one of the most prevalent viruses in potato crops (solanum tuberosum and s. phureja) around the world, causing reductions in crop yields between 10 and 20 %. symptoms of pvs infection may include leaf mottling, rugosity of leaves, deepening of the veins and reductions in crop yields between 10 and 20 %. virions are flexuous rods of 610-710 nm with a positive-sense ssrna genome of approximately 8500 nt comprising six orfs, a 5'ca ... | 2013 | 23665769 |
| complete genome sequence of the first andean strain of potato virus s from brazil and evidence of recombination between pvs strains. | an isolate of the andean strain of potato virus s (pvs), named bb-and, was detected for the first time in a brazilian potato crop, fully sequenced and analyzed. a comparison of bb-and with other pvs isolates (andean and ordinary) showed that bb-and is quite distinct. the lowest amino acid sequence identity to the only other fully sequenced andean isolate was found in orf 1 (82%) and orf 6 (87%). recombination analysis showed that the isolate vltava (aj863510), from germany, is a recombinant betw ... | 2012 | 22456909 |