| myxoviruses. | myxoviruses can be divided into 2 groups, orthomyxoviruses and paramyxoviruses. the former comprise the influenza group which is subdivided into types a,b and c. influenza b and c are purely human pathogens but influenza a, which includes a large number of antigenic subtypes, occurs in nature in pigs, horses, birds and man. all influenza a viruses irrespective of origin are chemically, biologically and genetically related. the epidemics which they cause are curious and puzzling and are an import ... | 1975 | 47825 |
| morphology and morphogenesis of a new paramyxovirus (pmv 107). | the morphology of the virions and nucleocapsids of paramyxovirus 107 (pvm 107) and the replication of the virus were investigated by electron microscopy. the virions and nucleocapsids exhibited the same structural properties as other paramyxoviruses. nuclecapsids were found in the nucleus and cytoplasm of infected bovine embryonic lung (bel) cell cultures. a similar situation has been described for the morbilliviruses measles, sspe, distemper and rinderpest. alignment of nucleocapsids beneath th ... | 1979 | 113501 |
| [comparative antigenic analysis of j-96 cell oncornavirus and mason-pfizer virus from a spontaneous monkey mammary tumor]. | a comparative study of the antigenic structure of oncornavirus of j-96 cells and mason-pfizer virus (m-pvm) isolated from cells of m. rhesus spontaneous mammary tumour was carried out using immunodiffusion in agar, immune autoradiography and indirect immunofluorescence procedures. immune rabbit serum to disrupted j-96 virus detected by agar immunodiffusion in preparations or purified virus three soluble antigens one of which was identical to group-specific m-pvm antigen. | 1976 | 180692 |
| enzyme-linked immunosorbent assay for the detection of antibodies to pneumonia virus of mice in rat sera. | an enzyme linked immunosorbent assay (elisa) was established to detect rat serum antibodies to pneumonia virus of mice (pvm). vero cells infected with the virus were absorbed to the surface of microplates, and the presence of antibodies in the sample evaluated were demonstrated by the subsequent use of goat anti-rat immune globulin g conjugated to peroxidase and by specific substrate. the results indicated that antibodies against pneumonia virus of mice could be detected in rat serum with the el ... | 1978 | 374862 |
| [serological examinations on natural infections with mouse pathogens in inbred mouse strains: difference in antibody detection among the strains (author's transl)]. | serological surveys on several infections were performed on the inbred mouse strains maintained at the central institute for experimental animals. in the first survey, 11 strains of mouse, which were 8 weeks of age or older and were kept in separate cages in the same animal room, were tested for antibodies to salmonella enteritidis, corynebacterium kutscheri, tyzzer's organisms, mycoplasma pulmonis, mouse hepatitis virus (mhv), sendai virus (hvj), pneumonia virus of mice (pvm) and minute virus o ... | 1978 | 631200 |
| pneumocystis carinii pneumonia in the rat model. | groups of barrier-raised but not certified virus-free sprague-dawley rats, obtained from the same source over the course of several years, were placed on an identical immunosuppressive regimen. this caused reactivation of latent pneumocystis carinii infection, manifest as p. carinii pneumonia (pcp) of varying severity. rats were euthanized after 9-12 wk of immunosuppression. an assessment of the severity of the induced pcp was made, based on the total number of organisms extracted from the lungs ... | 1991 | 1818139 |
| in vitro membrane binding of the translation products of the carlavirus 7-kda protein genes. | two double-stranded dna copies of the genes potentially coding for the 7-kda proteins of potato virus m (pvm) and potato virus s (pvs) were synthesized and cloned into t7 transcription vectors. cell-free translation of the corresponding monocistronic transcripts yielded in both cases a single protein of approximately 7-8 kda that contains a highly hydrophobic n-terminal segment. to analyze their membrane-binding potential, both proteins were synthesized in the membrane-enriched krebs-2 extract. ... | 1991 | 1853576 |
| genes 1 and 2 of pneumonia virus of mice encode proteins which have little homology with the 1c and 1b proteins of human respiratory syncytial virus. | genes 1 and 2 of pneumonia virus of mice (pvm) consist of 410 and 571 nucleotides and encode proteins of 113 and 156 amino acids respectively. the proteins show no extensive (gene 1 analogous to 1c) or low (gene 2 analogous to 1b) homology to their presumed counterparts in human respiratory syncytial virus (hrsv). the strongest homology is between regions of approximately 35 amino acids located near the carboxy termini of the gene 2 product and the 1b protein with 29% identity, although a lower ... | 1991 | 1919530 |
| [complete nucleotide sequence of genomic rna of the potato m-virus]. | the complete nucleotide sequence of potato virus m genomic rna has been determined to be 8534 nucleotides (with the exception of the poly(a) tail at the 3' end). the sequence contains six large open reading frames coding for proteins of mol. wt. 223206, 25438, 11893, 6793, 33906, and 12183 (in 5'----3' direction). according to its primary sequence analysis the 223k protein orf codes for a virus rna replicase. the in vitro translation product of 34k protein gene precipitates by the antisera again ... | 1991 | 1944258 |
| coat protein and protease activity as in vitro translation products of potato carlavirus m rna. | genomic-size rna isolated from purified potato carlavirus m (pvm) was translated in both the reticulocyte and the wheat germ cell-free, messenger-dependent systems. the pvm rna translated the same set of major products in both in vitro systems. the mr values of the most prominent polypeptides observed consistently were 185,000 (p185), 147,000 (p147), 94,000 (p94), 87,000 (p87), 72,000 (p72), 67,000 (p67), 52,000 (p52), 46,000 (p46), 35,000 (p35) and 25,000 (p25). relatively low amounts of a tran ... | 1991 | 1958128 |
| the genome organization of potato virus m rna. | the 8534 nucleotide sequence of the genome of the carlavirus, potato virus m (pvm), has been determined. the sequence contains six large open reading frames (orfs) and non-coding regions consisting of 75 nucleotides at the 5' end, 70 nucleotides followed by a poly(a) tail at the 3' end and 38 and 21 nucleotides between three large blocks of coding sequences. the orf beginning at the first initiation codon at nucleotide 76 encodes a polypeptide of 223k which, according to its primary sequence ana ... | 1991 | 1990070 |
| occurrence of chloroplast ribosome recognition sites within conserved elements of the rna genomes of carlaviruses. | the nucleotide sequences upstream from the carlavirus open reading frames were examined for direct sequence homology. blocks of homology were evident upstream from the 25 k orfs of potato virus s (pvs), potato virus m (pvm) and lily symptomless virus (lsv), and upstream from the coat protein initiation codons of pvs, pvm, lsv, carnation latent virus and helenium virus s. these blocks, which correspond to the 5'-terminal regions of the subgenomic rnas, were shown to contain potential ribosome rec ... | 1991 | 2013334 |
| immunotoxic effects of hexachlorobenzene on the pathogenesis of systemic, pneumonic and hepatic virus infections in the mouse. | a quantitative histopathological method has been developed for the evaluation of the effects of hexachlorobenzene (hcb) on the pathogenesis of three virus infections in the mouse. hexachlorobenzene was selected because a substantial amount of immunotoxicological data already exists with which we could compare our results. to establish the validity of the method a systemic virus infection (mouse cytomegalovirus, mcmv), a pneumonia causing virus (pneumonia virus of mice, pvm) and a hepatitis virus ... | 1990 | 2176797 |
| the putative rna replicase of potato virus m: obvious sequence similarity with potex- and tymoviruses. | on the basis of comparison of the protein sequences of the putative virus-specific replicases, carlaviruses can be placed in the "sindbis-like" supergroup of plus-stranded rna viruses. among these, the amino acid sequences of the replication proteins of potex- and tymoviruses showed the highest similarity to potato virus m. the possible functions of conserved domains are suggested to be methyltransferase, nucleotide-binding domain, and rna polymerase. | 1990 | 2238481 |
| the 12 kda protein of potato virus m displays properties of a nucleic acid-binding regulatory protein. | the 3' terminal 1.4 kb segment of potato virus m (pvm) genomic rna was cloned and sequenced. this part of the viral genome encodes the capsid protein cp as well as a 12 kda protein of as yet unknown function. both proteins were expressed in bacteria and their nucleic acid-binding properties studied. the 12 kda protein (pr12), but not the capsid protein bound single- and double-stranded nucleic acids. this property of pr12 in conjunction with a zinc finger motif located adjacent to a basic region ... | 1990 | 2265707 |
| a comparison of the nucleotide sequence homologies between isolates of the andean and ordinary strains of potato virus s and their relationship to other carlaviruses. | sequence homologies between the rnas of five isolates of the ordinary strain of potato virus s (pvso), four isolates of the andean strain (pvsa), potato virus m(pvm), and the type member of the carlavirus group, carnation latent virus (clv), were compared using cdna solution hybridization. a high degree of homology (90-100%) was detected between the majority of pvso and pvsa isolates, but lower levels of homology (less than 10%) were observed between pvs, pvm, and clv. | 1990 | 2270676 |
| molecular cloning of pneumonia virus of mice. | cdna clones representing nine genes of pneumonia virus of mice (pvm) have been generated. the sizes of the corresponding mrnas and a provisional transcriptional map of the virus genome have been determined. the apparent gene order is very similar to that of respiratory syncytial virus. the sequences adjacent to the 3' termini of the pvm genes were determined and are very similar to those of respiratory syncytial virus. several pvm gene polypeptide products have been assigned. | 1990 | 2319655 |
| seromonitoring in small laboratory animal colonies. a five year survey: 1984-1988. | from 1984 to 1988 one thousand serologic investigations of laboratory animal colonies originating from 10 different european countries were performed. the most prevalent infections in mouse stocks were caused by mouse hepatitis virus (mhv), minute virus of mice (mvm), theiler's encephalomyelitis virus (temv), reovirus type 3 (reo3), sendaivirus, and pneumonia virus of mice (pvm). in mice no infections with lymphocytic choriomeningitis virus (lcm), polyomavirus, mouse adenovirus, and k-virus were ... | 1990 | 2321435 |
| nicotiana velutina mosaic virus: evidence for a bipartite genome comprising 3 kb and 8 kb rnas. | dna complementary to nicotiana velutina mosaic virus (nvmv) rna was cloned and five segments larger than 0.9 kb were used in northern blot hybridization analysis to identify two virus-specific rnas, approximately 8 kb (rna 1) and 3 kb (rna 2) in size. the clones selected as probes did not hybridize with rna from various tobamoviruses, or from beet necrotic yellow vein (bnyvv) and peanut clump furoviruses. in an attempt to determine the taxonomic position of the virus, about 75% of the nvmv rna 2 ... | 1990 | 2345363 |
| [nucleotide sequence and structural organization of the 3'-terminal region of potato virus m]. | the sequence of 2630 3'-terminal nucleotides has been determined for the genomic rna of potato virus m (pvm), a type member of the carlavirus group. analysis of this nucleotide sequence revealed five open reading frames coding for proteins of mol. wt. 25, 12, 7, 34 and 11 kda (in 5'----3' direction). the pvm genome organization has been shown to be basically analogous to that of potexviruses, except that the latter at their 3' end lack the gene encoding 11k protein. amino acid sequence compariso ... | 1990 | 2362590 |
| nucleotide sequence of the 3'-terminal region of helenium virus s rna. | the sequence of 1390 nucleotides of the 3'-terminal region of helenium virus s rna was determined from cloned cdna. this portion of the viral rna contains two major open reading frames (orfs) encoding proteins of mr 32.9k and 12.6k. the 32.9k protein, which is similar in size to the capsid protein of the virus, shares marked homology with the coat proteins of potexviruses and two other carlaviruses. the orf, corresponding to mr 12.6k, nearest to the 3'-terminal poly(a) tail shows extensive simil ... | 1990 | 2391504 |
| chrysanthemum virus b: antibodies to structural protein in mammals and mg2(+)-dependent reverse-transcriptase activity. | human plasma has been found to contain antibodies reacting with the structural protein of chrysanthemum virus b (cv-b) and about 10 times less intensively with the structural protein of another carlavirus, the potato virus m. it has been shown that the antibodies bind to cv-b through their f(ab)2 fragments. no reaction with proteins of other plant viruses or retroviruses was observed. antibodies reacting with cv-b protein are also present in the plasma of green monkey, goat, rabbit, and mouse, t ... | 1989 | 2483603 |
| influence of viral infections on body weight, survival, and tumor prevalence in fischer 344/ncr rats on two-year studies. | sendai virus (sv), pneumonia virus of mice (pvm), and rat coronavirus/sialodacryoadenitis virus (rcv/sdav) were common viral infections of rats in the national cancer institute-national toxicology program (nci-ntp) studies from 1977 to 1983. influence of these viral infections on body weight, survival, and prevalences of spontaneous tumors in the f344/ncr rats of 28 diet control groups at five different laboratories were evaluated. tumor prevalences evaluated in this investigation included the f ... | 1989 | 2554057 |
| simultaneous quantification of two plant viruses by double-label time-resolved immunofluorometric assay. | for simultaneous and sensitive detection of two antigens in one sample, monoclonal antibody (mab) to potato virus m (pvm) was labelled with a lanthanide eu3+ and mab to potato virus x (pvx) with another lanthanide sm3+. a mixture of the labelled mabs was used as a tracer. after performing the immunoreactions, the fluorescence of the dissociated lanthanides was measured at different wave-lengths in a time-resolved fluorometer to quantificate the pvx and pvm amount in a sample. double-label time-r ... | 1989 | 2643612 |
| polypeptides of pneumonia virus of mice. i. immunological cross-reactions and post-translational modifications. | a murine polyclonal antiserum and monoclonal antibodies have been employed to identify pneumonia virus of mice (pvm) polypeptides in infected cells and to study post-translational modifications. immunoprecipitation experiments using a murine polyclonal antiserum and a monoclonal antibody directed against a 39k protein have established an antigenic relationship between two pvm proteins and the n and p proteins of human respiratory syncytial virus. although 20 virus-specific polypeptides have been ... | 1989 | 2732719 |
| polypeptides of pneumonia virus of mice. ii. characterization of the glycoproteins. | the kinetics of synthesis and the nature of the oligosaccharides of the glycoproteins of pneumonia virus of mice (pvm) were studied. tryptic peptide mapping showed that the two major glycosylated polypeptides g1 and g2 were different forms of the same protein. g2 was derived from g1 which in turn appeared to be derived from an unidentified precursor. the g1/g2 protein of pvm is probably a haemagglutinin since a monoclonal antibody directed against it has a high haemagglutination inhibition titre ... | 1989 | 2732720 |
| partial nucleotide sequence of potato virus m rna shows similarities to protexviruses in gene arrangement and the encoded amino acid sequences. | the nucleotide sequence of the 3'-proximal 2630 nucleotides of potato virus m (pvm) genomic rna was determined. the sequenced region contained five long open reading frames (orfs). the orf nearest to the 3'-terminal pol(a) tail corresponds to a polypeptide of mr 10,848. this orf is preceded by one which encodes a protein of mr 33,906 (34k) which has an amino acid sequence that is very similar in its carboxyterminal part to that of the coat proteins of some potexviruses. three other orfs encoding ... | 1989 | 2738581 |
| influence of viral infections on body weight, survival, and tumor prevalence of b6c3f1 (c57bl/6n x c3h/hen) mice in carcinogenicity studies. | sendai virus (sv), mouse hepatitis virus (mhv), and pneumonia virus of mice (pvm) are common viral infections of mice. influence of these viral infections on the prevalence of liver tumors, lung tumors, and lymphoma is of concern in chemical carcinogenicity studies. body weight, survival, and tumor prevalence of b6c3f1 mice with and without viral infections in 33 male and 34 female untreated control groups and 32 male and 32 female low- and high-dose groups of 2-year chemical carcinogenicity stu ... | 1989 | 2767355 |
| genetic and environmental control of diabetes induction by multi-dose streptozotocin in two balb/c substrains. | balb/cj male mice were resistant and balb/cbyj males were susceptible to induction of diabetes by multi-dose streptozotocin (msz). although both closely-related balb/c substrains expressed h-2d haplotype, they could be differentiated by allelic differences at three genetic loci [qa-2 (chr 17), bcd-1 (chr 5), and afr-1]. (balb/cj x balb/cbyj)f1 males inherited the balb/cj resistance phenotype in a dominant fashion, thereby eliminating the balb/cj-expressed afr-1b (recessive) allele as the suscept ... | 1988 | 2977107 |
| diagnosis of murine infections in relation to test methods employed. | comparative investigations of sendai virus, pneumonia virus of mice (pvm), mouse encephalomyelitis virus (mouse polio), minute virus of mice (mvm), and reovirus type 3 (reo 3) infected murine colonies revealed a 30% higher incidence of positive sera when enzyme-linked immunosorbent assay (elisa) was employed instead of hemagglutination inhibition (hi) tests. equivalent sensitivity as in the elisa was obtained when the same sera were investigated by indirect immunofluorescence (if) tests. the vir ... | 1986 | 3014210 |
| a serological survey on 15 murine pathogens in mice and rats. | a serological survey for 15 murine pathogens was performed on 269 mouse sera collected from 21 conventional and 12 barrier colonies, and on 376 rat sera collected from 21 conventional and 23 barrier colonies. animals having an antibody against at least one of the antigens were contained in 81.0% of conventional and 16.7% of barrier mouse colonies and also in 81.0% of conventional and 43.5% of barrier rat colonies. main contaminants were mouse hepatitis virus and sendai virus in mice, and sendai ... | 1986 | 3026818 |
| respiratory disease and wasting in athymic mice infected with pneumonia virus of mice. | although pneumonia virus of mice (pvm) is ubiquitous among rodent colonies in the united states, it has not been reported to cause clinically apparent disease in euthymic mice. however, pvm has been reported to cause respiratory disease and death in experimentally infected euthymic and athymic mice. a group of nu/nu mice, housed in quarantine in a trexler-type isolator, had weight loss and dyspnea. gross necropsy findings included cachexia and diffuse pulmonary edema or lobar consolidation. hist ... | 1988 | 3374086 |
| fatal pneumonia with terminal emaciation in nude mice caused by pneumonia virus of mice. | athymic nude mice used as sentinel animals in a mouse holding room died of pneumonia 17 to 32 weeks after being placed in the room. lesions in the pulmonary parenchyma consisted of monocytic exudate, epithelial cell necrosis, hemorrhage, fibrin deposition and interstitial fibrosis. septal edema, septal cell necrosis and septal capillary stasis were common, but there was limited sloughing of bronchial lining epithelium. indirect fluorescence microscopy (ifa) of lung sections using pneumonia virus ... | 1988 | 3411912 |
| murine pneumonia virus: seroepidemiological evidence of widespread human infection. | more than 75% of a random sample of adult human sera exhibited moderate to high murine pneumonia virus (pvm)-neutralizing activity. there was no correlation between pvm-neutralizing activity and respiratory syncytial virus or parainfluenza type 3 virus-neutralizing activities of the same sera. in children the proportion of sera with moderate to high titres increased with age, indicating early exposure to infection. seroconversion (i.e. greater than fourfold increase in titre) was observed in fou ... | 1986 | 3711866 |
| studies on pneumonia virus of mice (pvm) in cell culture. i. replication in baby hamster kidney cells and properties of the virus. | pneumonia virus of mice (pvm) has been serially propagated in a line of baby hamster kidney (bhk21) cells. a maximum titer of 6.3 x 10(6) tcid(50) per ml was obtained, and there was little variation in yield on serial passage. pvm grown in bhk21 cells was antigenically similar to virus obtained from the mouse lung, but was somewhat less virulent for the mouse after 10 serial passages in these cells. virus produced by bhk21 cells agglutinated mouse erythrocytes without prior heating or other trea ... | 1967 | 4165740 |
| studies on pneumonia virus of mice (pvm) in cell culture. ii. structure and morphogenesis of the virus particle. | pneumonia virus of mice (pvm) particles are spheres 80-120 mmicro in diameter, or filaments of similar diameter with lengths up to 3 micro. the particles possess an outer spike-covered envelope and helical internal component 120-150 a in diameter. virus particles acquire their envelope by a budding process at the cell membrane; mature particles are seen only extracellularly. dense inclusions are prominent in the cytoplasm of pvm-infected bhk21 cells by 48 hr after inoculation. the inclusions app ... | 1967 | 4165741 |
| comparative structure, morphogenesis and biological characteristics of the respiratory syncytial (rs) virus and the pneumonia virus of mice (pvm). | | 1974 | 4213002 |
| ultrastructure of potato infected with potato virus m. | | 1970 | 5460133 |
| alteration of viral respiratory infections of mice by prior infection with mouse hepatitis virus. | pre-infection with mouse hepatitis virus (mhv) strains s, 3, or jhm reduced the ability of mice to seroconvert to pvm. geometric mean antibody titers to pvm among mhv pre-infected mice were lower than those for control mice given only pvm, and dually infected mice seroconverted to pvm later than mice given pvm alone. pvm was not recovered from normally permissive respiratory tract tissues of mhv-s pre-infected mice. pre-infection of dba/2 mice with mhv-s compromised the susceptibility of these m ... | 1984 | 6097762 |
| a comparison in germ-free mice of the pathogenesis of sendai virus and mouse pneumonia virus infections. | the poathogenesis of sendai virus and pneumonia virus of mice (pvm) was studied using the immunoperoxidase technique on paraffin lung sections. the pathology of sendai virus corresponds to that of a bronchopneumonia, with virus demonstrated by immunoperoxidase in the bronchial epithelium, and sometimes in macrophages, for a period of 2--9 days post-infection. pneumonia virus of mice produces an interstitial pneumonia with virus demonstrated in the bronchial epithelium but also in the alveolar wa ... | 1980 | 6157018 |
| persistence of pneumonia virus of mice and sendai virus in germ-free (nu/nu) mice. | the pathogenicity and persistence of pneumonia virus of mice (pvm) and sendai virus has been studied using germ-free nu/nu mice. pvm was found to infect cells of the bronchial epithelium (and the alveolar wall) of the lungs of germ-free nu/nu mice using the immunoperoxidase technique. the virus was located in the bronchial epithelium for 11 days before elimination, but persisted in the alveolar wall for the duration of the experiment (20 days). after day 10 a humoral antibody response to pvm was ... | 1980 | 6252933 |
| response of weanling random-bred mice to infection with pneumonia virus of mice (pvm). | weanling random bred mice inoculated intranasally with 2,500 median tissue culture infectious doses of pneumonia virus of mice (pvm) had recoverable virus in the upper respiratory tract for the first 9 days post-infection. virus was isolated from the lungs only on day 5. seroconversion was first detected on day 9, and serum antibody titers continued to rise throughout the period of the study (24 days). lesions were confined to the nasal cavity of infected mice. susceptible contacts placed in cag ... | 1984 | 6716954 |
| experimental pneumovirus infections: 1. hydrocephalus of mice due to infection with pneumonia virus of mice (pvm). | the effects of the intracerebral inoculation of pneumonia virus of mice (pvm) into neonatal mice were investigated by light microscopy, immunofluorescence, and viral isolation. viral inclusions and virus-specific antigens, as demonstrated by immunofluorescence, were maximal on the 6th day after inoculation, revealing general infection of ependyma and very limited infection of the leptomeninges and the choroid plexus. hydrocephalus occurred on day 21 in 80% of inoculated mice. this report describ ... | 1980 | 7004199 |
| comparison of hemagglutination inhibition, serum neutralization and elisa-pvm tests for the detection of antibodies to pneumonia virus of mice in rat sera. | recently, the seroneutralization, hemagglutination inhibition, and the elisa procedures were used to determine the presence of serum antibody to the pneumonia virus of mice in rat sera. this study was initiated to evaluate the reliability of these procedures to detect specific antibodies in our laboratory animal species. the results obtained indicate that: 1) the seroneutralization test is not reliable since numerous false-positive sera were detected; 2) the elisa is more sensitive than both the ... | 1981 | 7276130 |
| translation in vitro of ribonucleic acids from potato virus x, potato virus m and white clover mosaic virus. | ribonucleic acids from three plant viruses: potato virus x (pvx), potato virus m (pvm) and white clover mosaic virus (wclmv) have been purified and used as messengers in a wheat germ cell-free system. it was demonstrated that each of these viruses contained heavy genomic rna species of mol. wt. about 2 x 10(6), which appeared to be very efficient templates for protein synthesis in vitro. the synthesis of high-molecular-weight polypeptides: 180 x 10(3), 190 x 10(3) and 170 x 10(3) was directed by ... | 1981 | 7324703 |
| new isolates of pneumonia virus of mice (pvm) from japanese rat colonies and their characterization. | two virus strains were isolated from the lungs of athymic rats and mice used as sentinel animals in 2 colonies of laboratory rats in japan in which antibodies to the pneumonia virus of mice (pvm) had been detected. the new isolates were identified as pvm by the following characteristics: rna virus, susceptibility to ether treatment, long filamentous viral structure in the cytoplasm of infected cells, and hemagglutinating activity in various erythrocytes, including those of mice and rats. in addi ... | 1995 | 7601231 |
| nucleotide sequences of the genes encoding the putative attachment glycoprotein (g) of mouse and tissue culture-passaged strains of pneumonia virus of mice. | the sequences of the genes encoding the putative attachment (g) proteins of pathogenic (strain j3666) mouse lung-passaged and nonpathogenic (strain 15) tissue culture-passaged strains of pneumonia virus of mice (pvm) have been determined. in both cases the major polypeptide was synthesised from the second open reading frame (orf), a feature also found in the g gene of respiratory syncytial (rs) virus, another pneumovirus. however, the orfs of the g genes of the two pvm strains were initiated at ... | 1995 | 7871733 |
| the nucleic acid-binding zinc finger protein of potato virus m is translated by internal initiation as well as by ribosomal frameshifting involving a shifty stop codon and a novel mechanism of p-site slippage. | the genes for the capsid protein cp and the nucleic acid-binding 12k protein (pr12) of potato virus m (pvm) constitute the 3' terminal gene cluster of the pvm rna genome. both proteins are presumably translated from a single subgenomic rna. we have identified two translational strategies operating in pr12 gene expression. internal initiation at the first and the second aug codon of the pr12 coding sequence results in the synthesis of the 12k protein. in addition the protein is produced as a cp/1 ... | 1994 | 7937111 |
| nucleotide sequence of the 7 k gene of helenium virus s. | the sequence of 380 nucleotides upstream of the start codon of the open reading frame (orf) of the coat protein of the carlavirus helenium virus (helvs) was determined from cloned cdna. this portion of the viral rna contained one complete orf encoding a protein of 7 k which had homology with similar sized proteins from other members of the carla- and potexvirus groups. sequence data was also obtained beyond the start codon of the 7 k orf into what was presumed to be the 12 k protein gene of the ... | 1993 | 8010191 |
| sequence of the phosphoprotein gene of pneumonia virus of mice: expression of multiple proteins from two overlapping reading frames. | the gene encoding the phosphoprotein of the pneumovirus pneumonia virus of mice (pvm) has been cloned and sequenced. the gene is 903 nucleotides in length and contains a long open reading frame (orf) capable of encoding a polypeptide of 295 amino acid residues. a smaller, second, overlapping orf encoding a polypeptide 137 amino acids in length was also present. the large orf directed the synthesis of a 39-kda polypeptide and four additional polypeptides with masses of 37 kda, 26 kda, 23 kda, and ... | 1994 | 8035533 |
| transmission of murine viruses and mycoplasma in laboratory mouse colonies with respect to housing conditions. | pathogen-free sentinel mice were placed in 7 animal rooms with different housing conditions and were serologically screened for antibodies to mouse hepatitis virus (mhv), pneumonia virus of mice (pvm), sendai virus, reovirus 3, theiler's mouse encephalomyelitis virus (tmev), ectromelia virus and mycoplasma pulmonis by enzyme-linked immunosorbent assays, at intervals after introduction. the most commonly detected antibody was against mhv, which was found in mice from 4 rooms, followed by pvm anti ... | 1994 | 8035561 |
| nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. | we have synthesized and mapped a library of cdna clones representing the rna genome of a strain of blueberry scorch carlavirus (bbscv) associated with a disease known locally, in new jersey, u.s.a., as sheep pen hill disease. the nucleotide sequence of that strain was determined to be 8514 residues, excluding the poly(a) tail. in addition, cdna clones representing the 3' terminus of another strain of the virus from the same field were synthesized, mapped and sequenced. the overall identity betwe ... | 1994 | 8151289 |
| serological evidence of pneumonia virus of mouse (pvm) infection in laboratory rats. | pvm specific antibody was determined using 3 serological tests (parker's original hemagglutination-inhibition [hi] test, modified hi test, and indirect fluorescent antibody [ifa] test). ifa test was found to be the most sensitive method, so that ifa test was considered to be the method of choice for a small scale microbiological monitoring in animal facilities. a total of 1,280 sera including rats, mice, hamsters and rabbits were tested for pvm antibody. the prevalence of pvm antibody was 17.2% ... | 1993 | 8354359 |
| mutual viral and bacterial infections after housing rats of various breeders within an experimental unit. | fifteen athymic rat strains from 11 breeding colonies were housed within an experimental facility for an immunological study. health status records supplied with 14 of the strains listed infections by kilham's rat virus (krv), clostridium piliforme (bacillus piliformis) and pasteurella pneumotropica for 2, 2 and 1 colonies respectively. in sera taken previous to the study from euthymic rats of 10 strains, antibodies to krv were detected in 3 strains, to pneumonia virus of mice (pvm), rat corona ... | 1996 | 8709572 |
| characterisation of the interaction between the nucleoprotein and phosphoprotein of pneumonia virus of mice. | a protein blotting technique was used to study the interaction occurring between the pneumonia virus of mice n protein and other pvm encoded proteins expressed in infected cells. measurement of the degree of binding indicated that the n protein specifically interacted only with the full-length 39 kda p protein in infected cells. truncated n-related proteins were synthesised in vitro and incubated with filter-bound full-length and truncated p proteins. the data suggested that many regions of the ... | 1995 | 8837886 |
| experimental pneumonia virus of mice infection of guineapigs spontaneously infected with bordetella bronchiseptica. | guineapigs that were intranasally inoculated with pneumonia virus of mice (pvm) seroconverted to pvm by 11 days post-infection. during the course of study (2-60 days post-infection) no gross or histologic lesions were identified within the lungs that could be attributed to pvm infection. mild rhinitis and tracheitis were found in most animals and acute purulent bronchopneumonia in two animals, which may have resulted from spontaneous subclinical bordetella bronchiseptica infection. viral and bac ... | 1997 | 9121108 |
| nucleotide sequence of the genes encoding the matrix and small hydrophobic proteins of pneumonia virus of mice. | the nucleotide sequences of the genes encoding the matrix (m) protein and the small hydrophobic (sh) protein of pneumonia virus of mice (pvm) are described. the matrix protein gene contains a large open reading frame encoding polypeptide of 257 residues which shows considerable (38.6-42.1%) amino acid identity with the matrix proteins of other pneumoviruses. the matrix gene also contains a second, smaller, open reading frame (orf) as seen with the equivalent genes of other pneumoviruses. the pvm ... | 1997 | 9140191 |
| movement protein-derived resistance to triple gene block-containing plant viruses. | two mutant potato virus x (pvx) movement protein (mp) genes (m 12k-sal and m 12k-kpn) were obtained by inserting specific linkers at the boundary between the n-terminal hydrophobic and putative transmembrane segment, and the central invariant hydrophilic region of the respective 12 kda, 12k, triple gene block (tgb) protein. several transgenic potato lines which expressed m 12k-sal or m 12k-kpn to different degrees were resistant to infection by pvx, potato aucuba mosaic potexvirus and the carlav ... | 1997 | 9191914 |
| phylogenetic analysis of triple gene block viruses based on the tgb 1 homolog gene indicates a convergent evolution. | the complete nucleotide sequence of the triple gene block one (tgb 1) of cymbidium mosaic potexvirus (cymmv) was compared to those from other potex-, carla-, furo- and hordeiviruses. seven conserved motifs in the tgb 1, including the atp-gtp binding domain (p-loop) consensus gxxgktsts, were found in all four virus genera. we propose that all tgbv can be classified into phylogenetic clusters based on their tgb 1 homolog genes. these clusters can be further delineated to form subgroups. the first ... | 1998 | 9654683 |
| nucleotide sequence and genome structure of grapevine rupestris stem pitting associated virus-1 reveal similarities to apple stem pitting virus. | rupestris stem pitting (rsp), a component of the rugose wood complex, is one of the most widespread graft-transmissible diseases of grapevines. here we report on the consistent association of a high molecular mass dsrna (ca. 8.7 kbp) with rsp. the dsrna was reverse-transcribed and cdnas generated were cloned into lambda zap ii. sequence analysis of the cdna clones showed that the dsrna was of viral origin and the putative virus was designated rupestris stem pitting associated virus-1 (rspav-1). ... | 1998 | 9714258 |
| pathogenesis of pneumovirus infections in mice: detection of pneumonia virus of mice and human respiratory syncytial virus mrna in lungs of infected mice by in situ hybridization. | the pathogenesis of pneumonia virus of mice (pvm) and human respiratory syncytial virus (hrsv) in balb/c mice were investigated by using in situ hybridization to detect virus mrna in fixed lung sections. following intranasal inoculation with 120 p.f.u. pvm the pattern of hybridization showed that virus mrna was initially detected within 2 days in alveolar cells. as the infection progressed the number of hybridizing alveolar cells increased and signal was also detected in cells lining the termina ... | 1998 | 9780046 |
| detection and characterization of proteins encoded by the second orf of the m2 gene of pneumoviruses. | the nucleotide sequence of the m2 gene of pneumonia virus of mice (pvm) was determined. the sequence showed that the gene encoded a protein of 176 amino acids with a predicted molecular mass of 20165 da from a major orf, which is smaller than the equivalent proteins encoded by human, bovine and ovine respiratory syncytial (rs) viruses. the pvm m2 protein is conserved, having 41% similarity to the equivalent human rs virus protein. in common with the m2 genes of the rs viruses and avian pneumovir ... | 1999 | 10466798 |
| pulmonary eosinophilia and production of mip-1alpha are prominent responses to infection with pneumonia virus of mice. | human eosinophils secrete two distinct ribonucleases that have antiviral activity against pathogens of the family paramyxoviridae. to examine the role of eosinophils and their ribonucleases in host defense against paramyxovirus pathogens in vivo, we have developed a mouse model involving a viral pathogen that naturally targets a rodent host. in this work we describe infection of balb/c mice with pneumonia virus of mice (pvm, strain j3666), a paramyxovirus pathogen found frequently among rodent p ... | 2000 | 10753501 |
| ten-year long monitoring of laboratory mouse and rat colonies in french facilities: a retrospective study. | from 1988 to 1997, a total of 69 mouse colonies and 36 rat colonies were examined for the presence of antibodies to 14 indigenous viruses of mice and rats. among mouse viruses, high positivity rates were observed with mouse hepatitis virus (mhv), theiler's encephalomyelitis virus (themv), minute virus of mice (mvm), sendai virus and pneumonia virus of mice (pvm); the prevalence rates were high in rats with khilam's rat virus (krv), themv, toolan's h-1 virus, sendai virus, parker's rat coronaviru ... | 2000 | 10759370 |
| the chemokine macrophage-inflammatory protein-1 alpha and its receptor ccr1 control pulmonary inflammation and antiviral host defense in paramyxovirus infection. | in this work, we explore the responses of specific gene-deleted mice to infection with the paramyxovirus pneumonia virus of mice (pvm). we have shown previously that infection of wild type mice with pvm results in pulmonary neutrophilia and eosinophilia accompanied by local production of macrophage-inflammatory protein-1 alpha (mip-1 alpha). here we examine the role of mip-1 alpha in the pathogenesis of this disease using mice deficient in mip-1 alpha or its receptor, ccr1. the inflammatory resp ... | 2000 | 10946298 |
| mip-1alpha is produced but it does not control pulmonary inflammation in response to respiratory syncytial virus infection in mice. | the intent of this study was to compare the cellular and biochemical inflammatory responses of mice infected with the paramyxovirus pathogens respiratory syncytial virus (rsv) and pneumonia virus of mice (pvm). although rsv is not a natural pathogen of mice, it has been used extensively in mouse models of the human disease, as a limited respiratory infection can be established via intranasal inoculation of virus at high titer. in earlier work, we found that acute infection with the natural roden ... | 2000 | 11161432 |
| molecular characterization of hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses. | the complete nucleotide sequence of the hop latent virus (hplv) genome was determined. the viral rna genome is 8,612 nucleotides long, excluding the poly(a) tail, and contains six open reading frames (orfs), which encode putative proteins of 224-kda (orf 1), 25-kda (orf 2), 11-kda (orf 3), 7-kda (orf 4), 34-kda (orf 5), and 12-kda (orf 6). orf 5 encodes the coat protein as demonstrated by n-terminal sequencing of three proteolytic peptides derived from the virus particle. the genome organization ... | 2000 | 11205102 |
| gene expression in epithelial cells in response to pneumovirus infection. | respiratory syncytial virus (rsv) and pneumonia virus of mice (pvm) are viruses of the family paramyxoviridae, subfamily pneumovirus, which cause clinically important respiratory infections in humans and rodents, respectively. the respiratory epithelial target cells respond to viral infection with specific alterations in gene expression, including production of chemoattractant cytokines, adhesion molecules, elements that are related to the apoptosis response, and others that remain incompletely ... | 2001 | 11686888 |
| eosinophils, eosinophil ribonucleases, and their role in host defense against respiratory virus pathogens. | eosinophils remain among the most enigmatic of cells, as our appreciation of their detrimental activities--e.g., asthma and allergic disease--far outweighs our understanding of their beneficial effects. among the major secretory effector proteins of eosinophils are the ribonucleases eosinophil-derived neurotoxin (edn) and eosinophil cationic protein (ecp) in primates and their orthologs, the eosinophil-associated ribonucleases (ears) in rodents. the rapid diversification observed among these rib ... | 2001 | 11698487 |
| molecular characterization of hop mosaic virus: its serological and molecular relationships to hop latent virus. | the 3'-terminal sequence of hop mosaic virus (hpmv) genomic rna was determined. a cdna of approximately 1.8 kbp was amplified from the hpmv genome by 3' race using a degenerate primer, which was designed to anneal to the overlapping region of open reading frames (orfs) 2 and 3 of eight carlavirus genomes. the sequence contained three orfs, encoding proteins of 7-, 34-, and 11-kda, which corresponded to orfs 4, 5, and 6 of the carlavirus genome, respectively. the amino acid sequence of orf 5, enc ... | 2001 | 11722015 |
| glucocorticoid administration accelerates mortality of pneumovirus-infected mice. | the use of glucocorticoids for the treatment of symptoms associated with respiratory syncytial virus (rsv) infection has been questioned. to evaluate the sequelae of glucocorticoid administration in the setting of pneumovirus infection in vivo, hydrocortisone was administered to mice infected with pneumonia virus of mice (pvm), a pneumovirus and natural rodent pathogen that is closely related to rsv and replicates the signs and symptoms of severe human rsv infection. results showed that hydrocor ... | 2001 | 11740726 |
| complete nucleotide sequence of the genomic rna of aconitum latent virus (genus carlavirus) isolated from delphinium sp.. | we determined the complete nucleotide sequence of aconitum latent virus (aclv-d) isolated from delphinium sp. the genomic rna of aclv-d is 8,657 nucleotides in length, excluding the poly (a) tail. based on computer analysis, six open reading frames (orfs) were identified as for other carlaviruses. although each orf differs from those of previously reported carlaviruses, the coat protein of aclv and potato virus m (pvm) shows a high level of identity. the results suggest that aclv is a distinct c ... | 2002 | 12038696 |
| differential expression of proinflammatory cytokine genes in vivo in response to pathogenic and nonpathogenic pneumovirus infections. | pneumonia virus of mice (pvm; paramyxoviridae, subfamily pneumovirinae) is an important pathogen for the study of physiologically relevant acute inflammatory responses in rodent hosts. in contrast to the severe symptomatology observed in response to infection with pvm strain j3666, infection with strain 15 resulted in few clinical symptoms, limited cellular inflammatory response, and no production of macrophage inflammatory protein-1alpha or monocyte chemoattractant peptide (mcp)-1. microarray a ... | 2002 | 12089656 |
| new host-virus relations between different solanum species and viruses. | we have studied the susceptibility or resistance of solanum capsicastrum link. et schauer, s. comatum sendt., s. dulcamara l., s. luteum mill., s. malacoxylon sendt. and s. nigrum l. to three aphid transmissible viruses [alfalfa mosaic alfamovirus (amv), potato m. carlavirus (pvm) and potato s carlavirus (pvs)]. out of the species, s. capsicastrum, s. comatum, s. dulcamara, s. malacoxylon and s. nigrum to amv, s. capsicastrum and s. malacoxylon to pvm and s. capsicastrum, s. luteum and s. nigrum ... | 2001 | 12425036 |
| characterization of a novel parainfluenza virus, caviid parainfluenza virus 3, from laboratory guinea pigs (cavia porcellus). | a novel respirovirus was isolated from nasopharyngeal swab specimens from clinically normal laboratory guinea pigs, and was characterized and named caviid parainfluenza virus 3 (cavpiv-3). the cavpiv-3 is enveloped, is 100 to 300 nm in diameter, and has a characteristic 15-nm-diameter chevron-shaped virus ribonucleocapsid protein. sequence analysis of the fusion glycoprotein of cavpiv-3 revealed it to be 94% identical to human and guinea pig parainfluenza 3 (piv-3) viruses and 80% identical to b ... | 2002 | 12540169 |
| identification of amino acids that are critical to the processivity function of respiratory syncytial virus m2-1 protein. | the m2-1 protein of respiratory syncytial virus (rsv) is a transcription processivity factor that is essential for virus replication. the function of rsv m2-1 protein can be examined by using an rsvlacz minigenome assay in vitro since the expression of the lacz gene is dependent on m2-1. the m2-1 protein of pneumonia virus of mice (pvm), also a member of the pneumovirus genus, functions poorly in the rsvlacz minigenome assay despite conservation of the cys(3)-his(1) motif at its n terminus and a ... | 2003 | 12692207 |
| detection of sendai virus and pneumonia virus of mice by use of fluorogenic nuclease reverse transcriptase polymerase chain reaction analysis. | sendai virus may induce acute respiratory tract disease in laboratory mice and is a common contaminant of biological materials. pneumonia virus of mice (pvm) also infects the respiratory tract and, like sendai virus, may induce a persistent wasting disease syndrome in immunodeficient mice. reverse transcriptase-polymerase chain reaction (rt-pcr) assays have proven useful for detection of sendai virus and pvm immunodeficient animals and contaminated biomaterials. fluorogenic nuclease rt-pcr assay ... | 2003 | 12784851 |
| diminished expression of an antiviral ribonuclease in response to pneumovirus infection in vivo. | the mouse eosinophil-associated ribonucleases (mears) are species specific, divergent orthologs of the human antiviral rnase a ribonucleases, eosinophil-derived neurotoxin (rnase 2) and eosinophil cationic protein (rnase 3). we show here that mear 2 is also an antiviral ribonuclease, as micromolar concentrations promote a approximately sixfold reduction in the infectivity of pneumonia virus of mice (pvm) for target respiratory epithelial cells in vitro. although initially identified as a compone ... | 2003 | 12927308 |
| effect of sodium monofluoroacetate on the multiplication of influenza viruses, mumps virus and pneumonia virus of mice (pvm). | sodium fluoroacetate, given after virus inoculation in doses of 3 to 4 mg. per kg. in mice or 2 mg. in chick embryos, caused only a slight delay in the multiplication of the pr8 strain of influenza a virus in the mouse lung and of pr8 or the lee strain of influenza b virus in the allantoic sac. the quantities of the compound used were sufficient to cause approximately 10 to 20 per cent mortality in mice and 100 per cent in chick embryos. the use of small virus inocula did not markedly increase t ... | 1952 | 13022848 |
| studies on primary atypical pneumonia. i. localization, isolation, and cultivation of a virus in chick embryos. | by means of fluorescein-labelled antibody, the primary atypical pneumonia virus was found to multiply exclusively in the cytoplasm of the epithelial cells lining the bronchioles and air sacs of developing chick embryos. when 13-day old embryos were inoculated intra-amniotically and incubated at 35 degrees c. for 5 days or longer, over 90 per cent of the inoculated embryos became infected. between 1954 and 1956, seven strains of pap virus were isolated from sputums or nasopharyngeal washings in p ... | 1957 | 13475605 |
| chimeric pneumovirus nucleocapsid (n) proteins allow identification of amino acids essential for the function of the respiratory syncytial virus n protein. | the nucleocapsid (n) protein of the pneumovirus respiratory syncytial virus (rsv) is a major structural protein which encapsidates the rna genome and is essential for replication and transcription of the rsv genome. the n protein of the related virus pneumonia virus of mice (pvm) is functionally unable to replace the rsv n protein in a minigenome replication assay. using chimeric proteins, in which the immediate c-terminal part of the rsv n protein was replaced with the equivalent region of the ... | 2003 | 13679601 |
| lack of identity of polyoma virus and pneumonia virus of mice (pvm). | | 1960 | 13828133 |
| pneumonia virus of mice (pvm) in cell culture. | | 1962 | 13980577 |
| potato virus m and paracrinkle. | | 1960 | 14403449 |
| the dependence of the pathological lesion upon the multiplication of pneumonia virus of mice (pvm); kinetic relation between the degree of viral multiplication and the extent of pneumonia. | the rate of multiplication of pvm in the mouse lung is relatively constant, averaging 7.9-fold per day with but slight variations, irrespective of the amount of virus inoculated. similarly, the rate of increase in the amount of pneumonia is relatively constant, averaging 4.7-fold per day, even though the quantity of virus inoculated is varied over a wide range. it follows that viral multiplication proceeds 1.7 times more rapidly than does extension of the pathological lesion, both reaching limit ... | 1951 | 14803638 |
| characteristics of the multiplication cycle of pneumonia virus of mice (pvm). | a study of the multiplication of pvm in the mouse lung yielded evidence in support of the hypothesis that discrete cycles occur. the cycle is characterized by a latent period of about 15 hours, an incremental period of 10 to 15 hours and a total duration of 24 to 30 hours. the yield of virus per cycle is approximately 16-fold. a large proportion, approximately 90 per cent, of pvm is lost on intranasal inoculation and cannot be recovered from the lung. of the virus which reaches the lung, almost ... | 1951 | 14803639 |
| therapy of infection with pneumonia virus of mice (pvm); effect of a polysaccharide on the multiplication cycles of the virus and on the course of the viral pneumonia. | inhibition of the multiplication of pvm by the capsular polysaccharide of friedländer bacillus, type b, is associated with restriction in the development of pneumonia induced with the virus in the mouse lung. the extent of the pneumonic process appears to be a function of the degree of viral multiplication; the greater the inhibition of multiplication, the less extensive is the pneumonia and the more probable is the recovery of animals treated with the polysaccharide. effective therapy of pneumo ... | 1951 | 14803640 |
| in vitro reactions of pneumonia virus of mice (pvm). | | 1951 | 14849618 |
| interference between mumps virus and pneumonia virus of mice (pvm) fate of mumps virus in the mouse lung. | | 1951 | 14888901 |
| quantitative aspects of the multiplication of influenza a virus in the mouse lung; relation between the degree of viral multiplication and the extent of pneumonia. | influenza a virus, pr8 strain, increases in amount in the infected mouse lung at a relatively constant rate. when more than 25 m.s.50 doses of virus is inoculated, the rate of multiplication appears to be independent of the amount of virus introduced; has a value of 1,100-fold increase per day. the rate of increase in the pulmonary lesions induced by infection of the mouse lung with pr8 also appears to be relatively constant and independent of the amount of virus inoculated; has a value of 8.5-f ... | 1952 | 14907966 |
| the enhancing effect of urethane on the severity of infection with pneumonia virus of mice. | urethane, given parenterally or orally to mice, increased the severity of pvm infection. not only were the lesions more extensive but mice could be infected with smaller inocula of virus and the multiplication of virus in the lung was enhanced. there was atrophy of lymphoid tissues but no suppression was noted of antibody formation. no relation could be found between pvm infection and the development of pulmonary adenomas in mice. | 1952 | 14907967 |
| a micro-test for measuring circulating antibodies against a virus (pvm). | | 1951 | 14908077 |
| effect of adrenal hormones on infection of mice with pneumonia virus of mice (pvm). | | 1951 | 14911933 |
| respiratory syncytial virus, pneumonia virus of mice, and influenza a virus differently affect respiratory allergy in mice. | respiratory viral infections in early childhood may interact with the immune system and modify allergen sensitization and/or allergic manifestations. in mice, respiratory syncytial virus (rsv) infection during allergic provocation aggravates the allergic t helper (th) 2 immune response, characterized by the production of il-4, il-5, and il-13, and inflammatory infiltrates. however, it is unclear whether the rsv-enhanced respiratory allergic response is a result of non-specific virus-induced dama ... | 2004 | 15005745 |
| further studies on the associative reactions of pneumonia virus of mice and influenza viruses; combination with various animal tissues and adsorbents. | tissue particles from a wide variety of animal tissues possess the capacity to combine with pvm. various adsorbents also combine with the virus. elution of pvm from combination with either tissue particles or adsorbents can be achieved by appropriately altering the ionic environment. influenza viruses also are adsorbed on and eluted from adsorbents under similar conditions. reasons are presented for thinking that combination of pvm with tissue particles results from the action of adsorptive forc ... | 1950 | 15395573 |
| reevaluation of the virulence of prototypic strain 15 of pneumonia virus of mice. | prototypic strain 15 of pneumonia virus of mice (pvm) has been described as being nonpathogenic in mice, in contrast to the mouse-passaged, highly virulent strain j3666. previous sequence analysis also indicated that strain 15 encodes an attachment g protein that is truncated at the amino terminus, which for the amino terminally anchored protein deletes the cytoplasmic tail. however, we found that pvm strain 15 obtained from the american type culture collection was highly virulent in mice and wa ... | 2004 | 15542688 |
| genome sequence of the non-pathogenic strain 15 of pneumonia virus of mice and comparison with the genome of the pathogenic strain j3666. | pneumonia virus of mice (pvm) is a member of the subfamily pneumovirinae and is the closest known relative of respiratory syncytial virus. both viruses cause pneumonia in their respective hosts. here, the genome sequences of two strains of pvm, non-pathogenic strain 15 and pathogenic strain j3666, are reported. comparison of the genome sequences revealed 59 nucleotide differences between the two strains, 37 of which were coding. the nucleotide differences were spread throughout the genome, affec ... | 2005 | 15604443 |
| the pneumonia virus of mice infection model for severe respiratory syncytial virus infection: identifying novel targets for therapeutic intervention. | pneumonia virus of mice (pvm) is the first infection model that replicates features of severe human respiratory syncytial virus (hrsv) disease in the mouse. the pvm model has highlighted the importance of inflammation to the pathogenesis of severe disease, demonstrating that the inflammatory response remains active and acute even when virus replication ceases in response to appropriate antiviral therapy. the fact that the inflammatory response continues and is not completely linked to ongoing vi ... | 2005 | 15626452 |
| complete sequence of the rna genome of pneumonia virus of mice (pvm). | pneumonia virus of mice (pvm) is an enveloped rna-containing virus of family paramyxoviridae. sequences had been determined previously for a number of pvm genes, although these represented cloned cdnas rather than consensus sequences. sequences were not available for the 3' -leader and 5' -trailer regions that constitute the genome termini or for the large polymerase l gene that accounts for 43% of the genome. also, the available sequences were from an attenuated variant of strain 15, whereas th ... | 2005 | 15744580 |
| experimental and natural weed host-virus relations. | weeds, as alternative hosts of plant viruses and nutrient plants of virus vectors play important role in virus ecology and epidemiology. the aim of our study was to discover new weed-virus relations. therefore some weed species were mechanically inoculated with 28 viruses (strains or isolates) maintained in our glasshouse. different weed species with and without visible symptoms were collected from agro-, water ecosystems and wastelands of hungary between 1997 and 2003. virus infections were eva ... | 2004 | 15759395 |