| nature and specificity of the rna-protein interaction in the case of the tymoviruses. | dissociation-reassociation experiments performed with turnip yellow mosaic virus in the presence of various rnas and polynucleotides were used to investigate the degree of specificity and the contribution of the associated rna moiety to the stability of tymv. the results emphasize the importance of strategic cytosine residues spread along the rna chain. some insight into the contribution of the protein could be gained from comparison of tymv and eggplant mosaic virus (emv), a virus similar to ty ... | 1976 | 13423 |
| reverse transcription of turnip yellow mosaic virus rna primed with calf-thymus dna hydrolysate: characterization of the purified cdna product. | complementary dna was transcribed from turnip yellow mosaic virus rna, using the method of taylor et al. (1). the purified cdna thus obtained sedimented between 2 and 4 s and was a mostly uniform transcript of template rna. it hybridized with a sharp transition to homologous tymv-rna (crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the rnas of two other tymoviruses, namely andean potato latent virus and eggplant mosaic virus. | 1978 | 82938 |
| translation of eggplant mosaic virus rna in wheat germ extracts and reticulocyte lysates. | | 1978 | 570320 |
| comparison of the strategies of expression of five tymovirus rnas by in vitro translation studies. | total nucleotide sequencing of the rna genome of various tymoviruses has demonstrated that the overall genome organization of these viruses is identical. furthermore, the strategies of expression of the turnip yellow mosaic virus (tymv) genome have been established by in vitro translation studies; these include the synthesis of a subgenomic rna, the utilization of overlapping open reading frames (orfs) and maturation of a polyprotein. in the experiments described here, the strategies of expressi ... | 1992 | 1538200 |
| primary structure of belladonna mottle virus coat protein. | the coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. the peptides were sequenced manually using the 4-n, n-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method. the chymotryptic peptides were aligned by overlapping sequences of tryptic peptides and by homology wi ... | 1989 | 2467911 |
| nucleotide sequence of the ononis yellow mosaic tymovirus genome. | the nucleotide sequence of the genome of ononis yellow mosaic tymovirus (oymv) has been determined. the genome is single-stranded rna, 6211 nucleotides long, and has three main open reading frames (orfs), two of them overlapping. the largest orf (nucleotides 179-5509) encodes a polyprotein of 1776 amino acid residues that has sequence similarities with polymerases of other viruses with rna genomes. the smaller overlapping orf (nucleotides 172-1965) encodes a protein of 597 amino acids of unknown ... | 1989 | 2800337 |
| antiviral effects of dihydroxypropyladenine [rs)-dhpa) and bromovinyldeoxyuridine (bvdu) on plant viruses. | two established antiviral agents, dihydroxypropyladenine (rs)-dhpa and bromovinyldeoxyuridine (bvdu) were evaluated for their inhibitory effects on 4 plant viruses: tobacco mosaic virus, potato virus x (pvx), eggplant mosaic virus (emv) and a potyvirus isolated from solanum palinacanthum (poty-sp). using the leaf disc incubation test, bvdu proved virtually inactive while (rs)-dhpa efficiently inhibited emv and poty-sp when applied at concentrations as low as 5 mg/l. tmv was less susceptible to t ... | 1987 | 3446016 |
| the classification of tymoviruses by cdna-rna hybridization and other measures of relatedness. | the relationships of twelve tymoviruses have been assessed by cdna-rna hybridization. in addition, the percentage molar nucleotide composition of the genome of the pd strain of kennedya yellow mosaic virus and the percentage molar amino acid composition of the coat proteins of cacao yellow mosaic, kennedya yellow mosaic and turnip yellow mosaic (cardamine strain) viruses were estimated. these as well as published serological comparisons and genome and coat protein composition determinations were ... | 1987 | 3662825 |
| exploratory numerical taxonomy based on biochemical and biophysical characters of the tymoviruses. | three different exploratory analytical techniques, principal components analysis, reciprocal averaging and cluster analysis, were used to examine taxonomic relationships among the tymoviruses. the three techniques gave similar but not identical interpretations of the interrelationships within this group. exploratory numerical analyses were useful in assessing relatedness, and they suggest that andean potato latent virus is misclassified as a strain of eggplant mosaic virus. | 1987 | 3667185 |
| the thermal stability and decapsidation mechanism of tymoviruses: a differential calorimetric study. | the thermal stability of virions present in purified suspensions of three tymoviruses, turnip yellow mosaic virus (tymv), belladonna mottle virus (belmv) and eggplant mosaic virus (emv) was investigated by microcalorimetry. virions are less stable than natural empty shells at 4.5 < or = ph < or = 8.5. polyvalent cations present in tymv stabilize the virions at ph < or = 5.0 only. virions decapsidate in three steps: i) the release of the viral rna, probably through a hole in the capsid; ii) the d ... | 1993 | 8286439 |
| infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cdna. | eggplant mosaic virus (emv) and ononis yellow mosaic virus (oymv) are two tymoviruses that have ssrna genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. full-length cdna clones of these viruses were constructed with a t7 promoter adjacent to the 5' terminus of the dna copy of the viral genome, and with unique restriction endonuclease sites at the 3' terminus. this allowed rna to be transcribed from the dna encoding the genome. the transcript rna ... | 1993 | 8328917 |
| transfer rna mimicry among tymoviral genomic rnas ranges from highly efficient to vestigial. | three trna-associated properties of a representative set of tymoviral rnas have been quantitatively assessed using higher plant (wheat germ) proteins: aminoacylation, ef-1alpha*gtp binding, and 3'-adenylation of 3'-cc forms of the rnas by ctp, atp:trna nucleotidyltransferase. the rnas fall into three classes differing in the extent of trna mimicry. turnip yellow mosaic (tymv) and kennedya yellow mosaic virus rnas had activities in all three properties similar to those of a higher plant trnavaltr ... | 1998 | 9742235 |
| the semipermeability of simple spherical virus capsids. | hydrogen-ion titration curves are reported for tomato bushy stunt virus, two strains of cowpea chlorotic mottle virus, and turnip crinkle virus, with particular attention to the hysteresis loops associated with the swelling and contraction of virions. there appears to be an archetypal shape of hysteresis loops, which is shared by viruses in several groups, suggestive of many intermediate states in the swelling of any one particle. in contrast, eggplant mosaic virus behaves as if its protein caps ... | 1984 | 18639804 |
| chayote mosaic virus, a new tymovirus infecting cucurbitaceae. | abstract chayote mosaic virus (chmv) is a putative tymovirus isolated from chayote crops in costa rica. chmv was characterized at the host range, serological, and molecular levels. chmv was transmitted mechanically and induced disease symptoms mainly in cucurbitaceae hosts. asymptomatic infections were detected in other host families. serologically, chmv is related to the andean potato latent virus (aplv) and the eggplant mosaic virus (emv), both members of the genus tymovirus infecting solanace ... | 2000 | 18944472 |
| aminoacylation and messenger functions of eggplant mosaic virus rna. | the predominant rna of eggplant mosaic virus (emv) was found to have a molecular weight of 1.9 x 10(6) by formamide gel electrophoresis. electrophoretic analysis of virion protein under dissociating conditions revealed two polypeptides, a major component of 21,000 daltons, and a minor component of 22,000 daltons. both peptides were present in translation products coded by rna isolated from virions, but the proportion of the 22,000-dalton peptide was higher in products synthesized using rna isola ... | 1979 | 18631604 |
| aminoacylation properties of eggplant mosaic virus rna. separation and association of trnas. | | 1978 | 695329 |
| the stability of eggplant mosaic virus: action of urea and alkaline ph on top and bottom components. | | 1977 | 17214 |
| self-assembly of eggplant mosaic virus protein. | | 1977 | 14438 |
| stucture of the amino-acid accepting 3'-end of high-molecular-weight eggplant mosaic virus rna. | the sequence of the first 59 nucleotides from the 3'-oh terminus of high-molecular-weight eggplant mosaic virus rna has been determined by standard radio-chemical techniques. the fragment was identified among the products of partial t1 rnase digestion by making use of the reverse migration, at ph 2.5, of the 3'-oh terminal oligonucleotide. no abnormal bases were found. a model of secondary structure may be constructed for this fragment, which is known to fix valine in the presence of valyl-trna ... | 1976 | 1062783 |
| the structure of eggplant mosaic virus: evidence for the presence of low molecular weight rna in top component. | | 1976 | 1258369 |
| amino acid accepting structures in tymovirus rnas. | we have shown that the 3' terminal nucleotide of tymovirus rna binds valine as do trnas. we have mainly studied eggplant mosaic virus which presents two types of particles. the heavy particle contains the genome which binds valine and a 4s satellite rna esterified by lysine. the light particle contains 4s rnas, possessing various acceptor capacities. among these 4s rnas we have identified the host plant trnasval, a single trna lys and probably the moiety of the genome which accepts valine. | 1976 | 1275405 |