| antiviral effect of apple beverages. | a variety of apple beverages were tested for antiviral activity against poliovirus 1 or coxsackievirus b5. freshly prepared apple juice was particularly antiviral, but its activity declined more readily than that of commercial juice in response to heat and storage. the component responsible for activity was located both in the pulp and skin; after ultrafiltration, activity was present in fractions greater and less than molecular weight 10,000. virus infectivity was not restored from virus-apple ... | 1978 | 32832 |
| a study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens. | infectious adenovirus type 5 and coxsackievirus type b5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees c for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin g. lipid-containing herpes simplex virus type 1 and respiratory s ... | 1976 | 61971 |
| brain and spinal cord lesions in pigs inoculated with swine vesicular disease (ukg strain) virus and coxsackievirus b5. | pigs inoculated intravenously with swine vesicular disease virus (ukg strain), those inoculated with coxsackievirus b5, and other pigs exposed by pen contact to the same viruses developed diffuse encephalomyelitis. perivascular cuffing, with lymphocytes and formation of neuroglia cell foci, were most prominent in telencephalon, diencephalon, and mesencephalon. encephalitis was of mild to severe intensity. severity of lesions was more extensive and severe in the pigs exposed to swine vesicular di ... | 1975 | 128305 |
| organ culture of human aorta: prolonged survival with support of viral replication. | organ cultures were established with use of human fetal aorta obtained after death; cellular elements were preserved in the cultures for periods of up to eight weeks, with maintenance of their in vivo tissue relationships. histologically, these cultures preserve the three coats of the aorta, including an intact endothelial layer cells. the aorta organ cultures supported the replication of echovirus 11, herpes simplex virus type 1, adenovirus 2, and coxsackievirus b5 without the production of det ... | 1975 | 165246 |
| virus detection on grapes. | grapes inoculated with poliovirus 1 and coxsackievirus b5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. these washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. | 1977 | 198080 |
| coxsackievirus b cardiopathy and angiopathy in the hypercholesterolemic host. | studies on the pathogenic potential of the human cardiotropic enterovirus, coxsackievirus b5, show that this agent localizes and replicates in the aorta of mice. nutritionally-induced hypercholesterolemia leads to an increased replication and persistence of virus in tissues, specifically the aorta. coxsackievirus b cardiopathy is markedly augmented in the hypercholesterolemic host, resulting in a persistent cardiomyolysis which is not evident in virus-infected animals with normal cholesterol lev ... | 1978 | 214092 |
| the relative effectiveness of commonly used disinfectants in inactivation of coxsackievirus b5. | coxsackievirus b5 in the presence of fetal calf serum was exposed to six commonly used disinfectants for times of 10, 20 and 30 s. at the end of exposure times skim milk neutralized the disinfectant activity, with residual virus assayed by the plaque technique. the six disinfectants considered were javex, sodium hydroxide, ethanol, wescodyne, one stroke ves-phene and sonacide. although 95% (v/v) ethanol was significantly more virucidal than dilutions of the other five disinfectants tested causin ... | 1978 | 215674 |
| migration of epithelial cells in the small intestine of mice perorally infected with coxsackievirus b5. | the rate of cell migration in the small intestine during enteric viral infections has not been assessed previously. cd-1 mice (33 days old) were infected perorally with 1.0 x 10(8) plague-forming units of coxsackievirus b5 and 12 hr later were injected intraperitoneally with 2 micron ci of [3h]thymidine/g of body weight. after 2, 12, 24, 48, 60, and 72 hr, mice were killed, and the small intestine was removed. specimens obtained at each interval were examined by radioautography; similar specimen ... | 1979 | 221595 |
| response of pigs to recent isolates of coxsackievirus b5. | | 1979 | 398243 |
| the relative resistance of f2 bacteriophage to inactivation by disinfectants. | f2 bacteriophage in the presence of fetal calf serum (at a final concentration of 10%) was exposed to six commonly used disinfectants for times of 10, 20 and 30 sec. at the end of exposure times skim milk neutralized the disinfectant activity and residual virus was assayed using the plaque technique. the 6 disinfectants considered were javex, sodium hydroxide, ethanol, wescodyne, one stroke ves-phene and sonacide. a 0.25% (w/v) solution of sodium hydroxide and 1/50 javex (1200 parts/10(6) chlori ... | 1979 | 458139 |
| tubular aggregation of coxsackievirus b5 in hela cells. | | 1977 | 888354 |
| infection of hypercholesterolemic mice with coxsackievirus b. | adult male mice were made hypercholesterolemic by a diet high in cholesterol, cholic acid, animal fat, and sucrose. after three months on this diet, animals were infected with 5 x 10(9) plaque-forming units of coxsackievirus b5. control groups consisted of uninfected hypercholesterolemic mice and infected mice maintained on a standard laboratory diet. infection in the hypercholesterolemic animals was associated with leukopenia, severe fatty metamorphosis and focal necrosis in the liver, cholelit ... | 1976 | 932493 |
| coxsackievirus b5 infection and aseptic meningitis in neonates and children. | in metropolitan washington, dc, an outbreak of aseptic meningitis in children was recognized in the summer and fall of 1972. age-specific attack rates were highest in children less than 1 year of age. the incidence of cases showed two peaks: one in july and another in october. coxsackievirus b5 was associated with cases occurring in july, august, and september, but was not implicated in the october cases. seventy-six percent of the confirmed coxsackievirus b5 infections in aseptic meningitis pat ... | 1975 | 1121962 |
| coxsackievirus b5 infections in acute oliguric renal failure. | six patients with acute oliguric renal failure were studied for detection of acute viral or mycoplasma infection. precipitating causes, such as streptococcal infection, use of nephrotoxic drugs, etc., were ruled out. tests for neurtralization and complement fixation antibodies, in which 19 viral and mycoplasma agents were used as antigens, demonstrated a recent coxsachievirus b5 infection in five of six patients. rises in titer to other antigens tested were not noted. a group of control patients ... | 1975 | 1159332 |
| hand, foot and mouth disease associated with coxsackievirus group b. | this study from frankfurt, although similar to other reports with respect to clinical findings, presents further evidence of a varied etiology for hand, foot and mouth disease (hfmd). of the 6 patients with hfmd, 5 ahd virologic evidence of coxsackievirus b5 infection, and 1 had definite evidence of coxsackievirus b2 infection. our data suggest an etiologic association of coxsackievirus group b with hfmd. | 1975 | 1179172 |
| cryptosporidium and coxsackievirus b5 causing epidemic diarrhoea in a child-care centre. | | 1992 | 1321327 |
| synthesis and biological activity of new heterocyclic structures: [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c] [1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo [4', 5': 4,5] pyrimido[6,1-b][1,3]thiazine. | some derivatives of thiazolo[3,2-c]pyrimidine, pyrimido[6,1-b][1,3]thiazine, thiazolo[2,3-i]purine, [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c][1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo[4',5':4,5]pyrimido[6,1-b][1,3]thiazine were prepared. the compounds were tested for antimicrobial and antimycotic activity on a number of strains, namely, escherichia coli, proteus vulgaris, p. mirabilis, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus, streptococcus faecalis, bacillus sub ... | 1992 | 1482518 |
| use of modified diatomaceous earth for removal and recovery of viruses in water. | diatomaceous earth was modified by in situ precipitation of metallic hydroxides. modification decreased the negative charge on the diatomaceous earth and increased its ability to adsorb viruses in water. electrostatic interactions were more important than hydrophobic interactions in virus adsorption to modified diatomaceous earth. filters containing diatomaceous earth modified by in situ precipitation of a combination of ferric chloride and aluminum chloride adsorbed greater than 80% of enterovi ... | 1991 | 1768124 |
| synthesis and biological activity of pyrimido[2,1-b][1,3]thiazine, [1,3]thiazino[3,2-a]purine and [1,2,3]triazolo[4,5-d][1,3]thiazino[3,2-a]pyrimidine derivatives and thiazole analogues. | some series of thiazolo[3,2-a]pyrimidine, pyrimido[2,1-b] [1,3]thiazine, thiazolo[3,2-a]purine, [1,3]thiazino[3,2-a]purine, thiazolo[3,2-a][1,2,3]triazolo[4,5-d]pyrimidine and [1,2,3]triazolo[4,5-d][1,3]thiazino[3,2-a]pyrimidine derivatives, variously functionalized, were prepared. the compounds were tested for antimicrobial and antimycotic activity on a number of strains, namely: e. coli, proteus vulgaris, p. mirabilis, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus, s. faecalis, ... | 1991 | 1793474 |
| increased incidence of insulin-dependent diabetes mellitus following an epidemic of coxsackievirus b5. | in order to address the hypothesis that coxsackievirus b5 is a cause of insulin-dependent diabetes mellitus (iddm), the incidence of iddm was examined following an epidemic of coxsackievirus b5 in jefferson county, alabama. incidence estimates were obtained from a registry of iddm which was maintained in the county since 1979. a total of 266 cases were diagnosed (69 black and 197 white individuals less than 20 years of age) between 1979 and 1988. significant increases in incidence were observed ... | 1991 | 1852097 |
| [diversity of etiological agent associated with aseptic meningitis--a survey on an epidemic in tajimi city, gifu prefecture in 1984]. | during the period from may to september in 1984, an epidemic of aseptic meningitis occurred in tajimi city, gifu prefecture. virological and serological investigations were performed. cases involved ranged from 0 to 12 years of age, and 83.1% of them were under 7 years of age. four types of enteroviruses were isolated from 38 to 59 cases (64.4%), and virus type isolated were coxsackie b1 (15 cases), b4 (2 cases), b5 (4 cases), echovirus 16 (14 cases), a mixture of coxsackie-virus b4 and echoviru ... | 1990 | 2172413 |
| inhibition of the uncoating of bovine enterovirus by short chain fatty acids. | short chain fatty acids inhibit the replication of bovine enterovirus but are almost ineffective against poliovirus type 1, coxsackievirus b5, encephalomyocarditis virus and human rhinovirus 1b. lauric acid binds to bovine enterovirus, thereby stabilizing the virus particle to heat degradation. fatty acid-bound virions attach to susceptible cells but fail to undergo cell-mediated uncoating. the inhibitory effect is reversible with chloroform and may result from a hydrophobic interaction between ... | 1990 | 2172446 |
| isolation of enterovirus and reovirus from sewage and treated effluents in selected puerto rican communities. | sewage treatment plant effluents were surveyed for viral contributions to gastroenteritis outbreaks in puerto rico. of the 15 sewage treatment plants studied, all discharged their effluents upstream from water treatment plant intakes. no base-line data on the degree of viral challenge to these sewage treatment plants or the subsequent reduction of viruses before discharge existed. enterovirus counts were generally much higher than those found in the continental united states. at four plants, vir ... | 1989 | 2541664 |
| enterovirus replication in porcine ileal explants. | organ explants of porcine ileum were cultured in different media for up to 48 h. tissue preservation was evaluated by light microscopy and by transmission and scanning electron microscopy. cellular structure was well maintained after incubation for 48 h in cmrl-1066 supplemented with insulin and cortisone. explants of absorptive or lymphoid tissue from young or adult pigs were incubated with either coxsackievirus b5 (which is infectious for swine) or human poliovirus type 1 (which served as a co ... | 1987 | 2821187 |
| inactivation of animal viruses during sewage sludge treatment. | using a previously developed filter adsorption technique, the inactivation of a human rotavirus, a coxsackievirus b5, and a bovine parvovirus was monitored during sludge treatment processes. during conventional anaerobic mesophilic digestion at 35 to 36 degrees c, only minor inactivation of all three viruses occurred. the k' values measured were 0.314 log10 unit/day for rotavirus, 0.475 log10 unit/day for coxsackievirus b5, and 0.944 log10 unit/day for parvovirus. however, anaerobic thermophilic ... | 1987 | 2823708 |
| coxsackievirus-cell interactions that initiate infection in porcine ileal explants. | coxsackievirus b5 (cb5) labeled with tritiated uridine was used to trace the interaction of the virus with explant cultures of porcine ileum. similarly labeled human poliovirus 1 (po 1), which is not specifically retained by porcine tissue, was used as a control. the explant procedure employed could maintain ileal tissue in a differentiated state for up to 48 hours. porcine ileum was acquired from both young (4-6 week-old) and adult (9-11 month-old) animals. inoculated explants of either absorpt ... | 1988 | 2843148 |
| relative resistance to chlorine of poliovirus and coxsackievirus isolates from environmental sources and drinking water. | several poliovirus and coxsackievirus isolates from environmental sources were compared with laboratory strains to determine their rate of inactivation by chlorine. all viruses were tested for up to 1,000 min in the presence of an initial free residual chlorine level of ca. 0.4 mg/liter. coxsackievirus b5 (cb-5) isolates were found to be more resistant to chlorine than coxsackievirus b4 (cb-4), followed by poliovirus 1, 2, and 3 in order of decreasing resistance to chlorine. environmental isolat ... | 1985 | 2988441 |
| concentration of viruses from water by using cellulose filters modified by in situ precipitation of ferric and aluminum hydroxides. | untreated cellulose filters adsorbed only small amounts of poliovirus 1, echovirus 5, coxsackievirus b5, or bacteriophage ms2 that were added to tap water or to solutions of imidazole-glycine buffer at ph 5 to 7. modification of filters by in situ flocculation of ferric and aluminum hydroxides greatly increased the ability of the filters to adsorb viruses. viruses adsorbed to the modified filters could be recovered by treating the filters with 3% beef extract (ph 9.5). greater than 60% of the en ... | 1985 | 3004332 |
| coxsackievirus-positive cervices in women with febrile illnesses during the third trimester in pregnancy. | coxsackievirus b5 infection was demonstrated in five of seven third-trimester pregnant women with undifferentiated febrile illnesses or aseptic meningitis. coxsackievirus b5 was recovered from the cervix and throat in four women and from the rectum in three. no obvious illnesses were evident in the babies. these findings suggest that previously unrecognized cervical enterovirus carriage or infection is common in infected pregnant women in the last trimester and that subsequent neonatal infection ... | 1986 | 3014880 |
| reemergence of an epidemic coxsackievirus b5 genotype. | outbreaks of coxsackievirus b5 (cb5) infections occur primarily during peak epidemic years, with comparatively few cases occurring during intervening years. this pattern of periodic cb5 epidemicity is quite distinct from the general endemicity typical of other group b coxsackieviruses. to determine the genetic relationships among cb5 isolates from different outbreaks, we compared viral rnas by ribonuclease t1 oligonucleotide fingerprinting. isolates obtained within an epidemic year had very simi ... | 1987 | 3036966 |
| fatal hepatitis in siblings: isolation of coxsackievirus b5 and herpes simplex virus. | | 1970 | 4316279 |
| enterovirus recovery with vegetable floc. | a lettuce floc was prepared and used for recovering enterovirus from an aqueous suspension. the method is simple, and the adsorption of coxsackievirus b5, echovirus 7, and poliovirus 1 is quantitative. the virus-floc complex may be removed from aqueous suspension by low-speed centrifugation and dissolved at an alkaline ph in a small volume of water; virus is then available for assay on cultured cells. flocs from some other green vegetables also possess the property of virus adsorption | 1973 | 4356468 |
| a community and intensive care nursery outbreak of coxsackievirus b5 meningitis. | | 1974 | 4809793 |
| [acquisition of infectious power by coxsackievirus b5]. | | 1970 | 4986363 |
| an outbreak caused by coxsackievirus b5 among newborn infants. | | 1972 | 5021406 |
| persistance of neutralizing antibody to coxsackievirus b5 following aseptic meningitis. | | 1965 | 5294928 |
| the enhanced release of coxsackievirus b5 from a trypsinized monolayer of hep-2 cells. | | 1968 | 5644403 |
| virus enhancement with a growth product of streptomyces scabies. ii. increased release of coxsackievirus b5 from hep-2 monolayers treated with scaben. | | 1968 | 5681522 |
| mercaptoethanol-sensitive neutralizing-antibody in natural infection with coxsackievirus b5. | | 1966 | 5918940 |
| studies on plaque variants of coxsackievirus b5 by burst analyses. | | 1967 | 6035534 |
| replicase gene of coxsackievirus b3. | a cdna copy covering two-thirds of the coxsackievirus b3 genome was cloned in the psti site of the pbr322 vector. a nucleotide sequence containing the gene for the viral replicase and the 3' noncoding region of the coxsackievirus b3 genome was determined. the predicted amino acid sequence of the coxsackievirus b3 replicase was shown to be remarkably similar to that of the poliovirus 1 replicase. the 3' noncoding region, in contrast, was only weakly homologous to the poliovirus 1 sequence but sho ... | 1984 | 6088796 |
| enteroviruses in vermont, 1969-1978: an important cause of illness throughout the year. | enteroviruses continue to play an important role in both epidemic and sporadic febrile diseases. of the clinical specimens submitted for diagnosis during a 10-year period (1969-1978) at the medical center of vermont hospital, burlington, a wide variety of diseases were associated with the isolation of enteroviruses. among these diseases were aseptic meningitis, hand-foot-and-mouth disease, diarrhea, pleurodynia, myocarditis, herpangia, and fevers. in 1972 a single enterovirus serotype, coxsackie ... | 1980 | 6245151 |
| comparison of proliferation and cytopathogenicity of swine vesicular virus and coxsackievirus b5. | sequential appearance of both swine vesicular disease virus and coxsackievirus b5 antigens in a pig kidney cell line was studied by immunofluorescence and electron microscopy. the replication cycle of each virus was approximately 3-4 h. viral antigens were demonstrable in the cytoplasm 2 h after inoculation. a compact mass of fluorescence was seen when cells showed cytopathogenic effect at 5.5 h. after 3 h, a few viral particles, seen by electron microscopy, were in the cytoplasm. morphological ... | 1981 | 6269809 |
| [infections of the central nervous system caused by enterovirus: 223 cases seen at a pediatric hospital between 1973 and 1981]. | between 1973 and 1981, 223 patients were seen at hôpital sainte-justine in montreal for enteroviral infection of the nervous system. in 161 the cause was documented by isolation of an enterovirus from the cerebrospinal fluid (csf). the viruses most frequently isolated were echovirus 11 (36 isolates), echovirus 30 (24), coxsackievirus b2 (23), coxsackievirus b3 (19), echovirus 6 (18), coxsackievirus b5 (16), coxsackievirus a9 (15), echovirus 9 (13), echovirus 7 (12) and coxsackievirus b1 (11). as ... | 1982 | 6291736 |
| concentration of rotavirus and enteroviruses from blue crabs (callinectes sapidus). | a simple method for concentration and detection of rotavirus and enteroviruses in the blue crab is described. virus was separated from tissue homogenates at ph 9.5, concentrated by adsorption to protein precipitates at ph 3.5, and recovered by elution of precipitates at ph 9.2. test samples of 12 to 15 ml were produced from an initial 100 g of crab tissues. cat-floc precipitation was used to remove sample toxicity for cell cultures. recovery effectiveness averaged 52% with poliovirus 1, echoviru ... | 1983 | 6318667 |
| coxsackievirus b5 meningitis--texas, 1983. | | 1984 | 6425630 |
| risk of infection associated with a wastewater spray irrigation system used for farming. | the use of wastewater for agricultural purposes involves the potential risk of infection from microorganisms in the wastewater. the application of partially treated wastewater on farms has been reported in one study to be associated with human illness, but this has not been confirmed. in the present study, workers at a land application system involving low-pressure spray irrigation of corn fields with wastewater were followed through a growing season to determine if they had an increased risk of ... | 1984 | 6694008 |
| bleomycin differentially inhibits picornavirus, herpesvirus and poxvirus replication in a human carcinoma cell line. | ms-757, a cervix carcinoma cell line, was exposed to bleomycin at concentrations up to 200 microm for periods of 1 to 24 hours. bleomycin treatment caused the level of dna synthesis in uninfected cells to drop to 13% of the level achieved in the controls. protein synthesis fell to 50%, but rna synthesis was not affected. exposure of uninfected cells to 200 microm bleomycin for 24 h did not induce significant cell death measured as permeability to trypan blue). a tetrazolium dye-reduction assay, ... | 1993 | 7514894 |
| immunoglobulin m capture immunoassay in investigation of coxsackievirus b5 and b6 outbreaks in south australia. | an immunoglobulin m (igm) capture enzyme immunoassay was used to detect major overlapping outbreaks of disease in south australia caused by coxsackieviruses b5 (cbv-5) and b6 (cbv-6). cbv-5-specific igm was detected in patients presenting in spring 1992 with acute febrile illnesses, rash, severe acute respiratory disease, meningitis, myocarditis and/or pericarditis, while tests for other viruses were negative. cbv-5 was isolated from an early case. in december 1992 it was noted that cbv-6 had re ... | 1995 | 7650200 |
| complete nucleotide sequence of a coxsackie b5 virus and its relationship to swine vesicular disease virus. | we report the first complete nucleotide sequence of the picornavirus coxsackievirus b5 (cb5), strain 1954/uk/85, an isolate from a case of hand-foot-and-mouth disease. we have compared the sequence with those of other coxsackie b viruses, coxsackievirus a9, poliovirus and swine vesicular disease virus (svdv). the genes encoding the three major capsid proteins are most closely related to those of svdv but the 5' and 3' noncoding regions and the p3 gene are more similar to the corresponding region ... | 1993 | 8388019 |
| comparison of glass wool and glass powder methods for concentration of viruses from treated waste waters. | enumeration of cultivable virus particle in sewage requires the samples to be concentrated. two adsorption-elution methods, the glass wool cartridge method and the glass powder fluid layer method were compared. firstly, it was demonstrated that virus could be readily recovered from the head, first 25 ml, of eluate of glass wool rather than from a reconcentration of the entire eluate, either by organic flocculation: 83% of positivity vs 44% respectively or double precipitation by peg: 85% of posi ... | 1993 | 8476496 |
| genotypic variation in coxsackievirus b5 isolates from three different outbreaks in the united states. | genomic sequences in vp1/2a and 5'-non-coding region of 10 isolates of coxsackievirus b5 from three outbreaks were compared with published sequences of another coxsackievirus b5, swine vesicular disease virus, coxsackievirus b1, coxsackievirus b3, and coxsackievirus b4. isolates of coxsackievirus b5 from the same outbreak showed close relations, not exceeding 7.2% in nucleotide differences. differences were greater between isolates from different outbreaks, varying between 8.4 and 16%. we have a ... | 1995 | 8578854 |
| coxsackievirus-associated pancreatitis mimicking metastatic carcinoma. | the origin of idiopathic acute pancreatitis has not been well defined. several enteroviruses, including coxsackievirus, have been implicated. we report the case of a 21-year-old woman with severe, acute pancreatitis with no classic risk factors. initial evaluation failed to show choledocholithiasis. her condition improved with conservative treatment, but symptoms recurred after resumption of oral intake. computed tomography (ct) of the abdomen revealed an atypical pseudocyst and omental nodules ... | 1996 | 8614892 |
| the detection of enteroviruses in large volume concentrates of recreational waters by the polymerase chain reaction. | a rapid and simple method was developed to detect enteroviruses in large-volume water samples. it relies on the adsorption of the virus capsids to silica particles under acidic conditions, allowing their recovery by relatively gentle centrifugation. different reagents used in enterovirus concentration and detection were seeded with coxsackievirus b5 and used to optimise the recovery method, which was then used to detect the enteroviruses from seeded and unseeded 101 seawater samples in one pcr t ... | 1997 | 9274818 |
| coxsackievirus b5 and the relationship to swine vesicular disease virus. | | 1997 | 9294928 |
| [viral meningoencephalitis caused by enterovirus in cuba from 1990-1995]. | the results of a study on enterovirus as causal agents of viral meningoencephalitis from 1990 to 1995 are described. from the 1458 samples of patients studied, 586 were from feces, 108 from cerebrospinal fluid and 1095 were paired sera. the samples for viral isolation were inoculated in two different cellular systems. a total of 225 samples (32.42%) were found to be positive; the highest number of isolations (n = 217) was found in feces and in diploid human lung fibroblast cells (phue-1). antibo ... | 1997 | 9472139 |
| [outbreak of coxsackievirus b5 meningoencephalitis in cuba, 1995]. | an increase in the reported cases of viral meningoencephalitis (vme) was detected in october and november, 1995, compared with the same period of 1994. 43 stock specimens from children with this diagnosis were received at the laboratory of enterovirus from the "pedro kourí" institute of tropical medicine. 23 isolations (53.4%) were obtained and identified as coxsackievirus b5. besides, in 43 matched sera investigated by the neutralization test against some enteroviruses, 21 proved to be positive ... | 1997 | 9685965 |
| several different enterovirus serotypes can be associated with prediabetic autoimmune episodes and onset of overt iddm. childhood diabetes in finland (dime) study group. | in a prospective multicentre study described previously on prediabetic events in siblings of index cases with insulin-dependent diabetes mellitus, 31 children developed clinical diabetes during the observation period and 51 children seroconverted for islet cell antibodies or insulin autoantibodies. by using nonserotype specific eia and ria, it has shown recently that enterovirus infections in both groups were frequently associated with increases of islet cell antibody and/or insulin autoantibody ... | 1998 | 9700636 |
| [outbreak of viral meningoencephalitis. comparison of 2 biological systems used for isolation of the virus]. | an increase of the cases of viral meningoencephalitis was reported in our country from october to december, 1995. 23 faeces specimens were received from 2 provinces aimed at obtaining the viral isolation by their inoculation in cell culture and in newborn mice at the same time in order to compare the sensitivity of both systems in isolation. a higher number of isolations was obtained in cell culture. the cell culture from kidney of a normal adult green african monkey (vero) proved to be more sen ... | 1998 | 9842272 |
| enterovirus infections as a possible risk factor for myocardial infarction. | an increasing body of evidence suggests that, in addition to the well-known classic risk factors, some microbial infections may be associated with the development of atherosclerosis and myocardial infarction (mi). the aim of our study was to evaluate the possible role of enteroviral infections in the pathogenesis of mi. | 1998 | 9843459 |
| severe hepatitis in a newborn with coxsackievirus b5 infection. | | 1999 | 10618768 |
| the mechanism of chronic coxsackievirus b hepatitis in rabbits. | the pathophysiology of chronic hepatitis in rabbits infected with coxsackievirus b5 (cvb5), (strain mitchell) was investigated. three-week-old male new zealand white rabbits were inoculated intraperitoneally with 1 x 10(5) plaque forming units of virus. every 3 months for 15 months postinoculation (p.i.) groups of animals were sacrificed for the following tests: interleukin (il)-6, tumor necrosis factor-alpha (tnf-alpha) and interleukin (il)-beta cytokine levels by enzyme-linked immunosorbent as ... | 1999 | 10630792 |
| molecular analysis of the prototype coxsackievirus b5 genome. | to facilitate studies of the phylogenetic relationship between enteroviruses, in particular the prototype strain (faulkner) of coxsackievirus b5 (cvb5f) and other cvb5 isolates and to facilitate studies of the interactions between cvb5f and the target cell, the complete nucleotide sequence of the prototype has been determined. the complete sequence was collected from three overlapping reverse transcription polymerase chain reaction (rt-pcr) generated amplicons. molecular analysis of the cvb5f ge ... | 2000 | 10752549 |
| [treatment plants: viruses in aerosols]. | a survey was carried out on the animal virus occurrence in wastewater and aerosols, in 2 treatment plants. forty-five samples of sewage (10 litres) and 118 aerosol (2.3 m3) were examined. forty out of forty-five (88.9%) of sewage and four out of one hundred and eighteen of aerosol (3.4%) samples were positive for viruses. thirty-three reovirus and nine enterovirus strains were recovered from the sewage, two reovirus and two enterovirus strains from aerosol. among enterovirus strains 6 resulted c ... | 1999 | 10771712 |
| singleton reactors in the diagnosis of swine vesicular disease: the role of coxsackievirus b5. | swine vesicular disease virus (svdv) and coxsackie b5 virus (cvb5) are closely related viruses that can infect swine and man and give rise to cross-reacting serum antibodies. it is, therefore, possible that svd antibodies found in serologic screenings of pigs are induced by cvb5. single positive animals found in screening programmes are generally referred to as singleton reactors (sr). to determine whether sr in svdv screenings are induced by cvb5 infection, virus neutralisation tests (vnts) and ... | 2000 | 10973703 |
| enteroviruses in spain: virological and epidemiological studies over 10 years (1988-97). | a total of 15,662 clinical samples were analysed for enterovirus (ev) isolation in cell cultures during a 10-year period (1988-97). furthermore, 210 isolates of ev obtained in primary laboratories within spain from patients with meningitis were characterized. the total number of ev typed was 758, including 727 non-polio ev and 31 sabin-like (sl) polioviruses. twenty-eight ev serotypes were represented. echoviruses comprised 90% (653/727) of fully typed non-polio ev. the four most prevalent serot ... | 2000 | 10982074 |
| swine vesicular disease: an overview. | swine vesicular disease (svd) is a notifiable viral disease of pigs included on the office international des epizooties list a. the first outbreak of the disease was recognized in italy in 1966. subsequently, the disease has been reported in many european and asian countries. the causative agent of the disease is svd virus which is currently classified as a porcine variant of human coxsackievirus b5 and a member of the genus enterovirus in the family picornaviridae. from a clinical point of view ... | 2000 | 11061956 |
| isolation of coxsackievirus b5 from pigs. | a cytopathic virus was isolated from young pigs suffering from severe diarrhea in okinawa, japan in 1986. the disease was highly contagious among young pigs. the physico-chemical properties of the virus indicated an enterovirus, but, no of serological relationship was detected with reference strains of porcine enteroviruses. with the aid of genbank for genomic sequence data, rt-pcr and hybridization method was performed. the viral isolate was identified as the coxsackievirus (cv) b5 of human ent ... | 2001 | 11497077 |
| swine vesicular disease virus. pathology of the disease and molecular characteristics of the virion. | swine vesicular disease is a highly contagious disease of pigs that is caused by an enterovirus of the family picornaviridae. the virus is a relatively recent derivative of the human coxsackievirus b5, with which it has high molecular and antigenic homology. the disease is not severe, and affected animals usually show moderate general weakening and slight weight loss that is recovered in few days, as well as vesicular lesions in the mucosa of the mouth and nose and in the interdigital spaces of ... | 2000 | 11708597 |
| encephalitis and myocarditis in a child with acute lymphoblastic leukemia: role of coxsackievirus b5? | enteroviruses are common causes of viral encephalitis in childhood and the most common cause of myocarditis. the prognosis is good with exception of the immunocompromised children who are at higher risk with increased mortality. a case of a 7-year-old boy with acute lymphoblastic leukemia and coxsackievirus b5-associated encephalitis and myocarditis is described. the boy was in complete remission and coxsackievirus b5 infection occurred 22 months after the beginning of chemotherapy. the clinical ... | 2002 | 11936734 |
| mapping of linear epitopes on the capsid proteins of swine vesicular disease virus using monoclonal antibodies. | the antigenic linear map of swine vesicular disease virus (svdv) has been studied using a repertoire of monoclonal antibodies (mabs) raised against a recombinant svdv polyprotein, p1. peptide-scanning analyses, cross-reactivity studies with homologous and heterologous viruses and predicted location on a computer-generated three-dimensional model of the capsid proteins have allowed the identification of five main linear sites. two sites, the n terminus of vp3 and amino acids 51-60 on vp1, corresp ... | 2002 | 12029154 |
| maternal first-trimester enterovirus infection and future risk of type 1 diabetes in the exposed fetus. | previous studies have suggested that enterovirus infections during pregnancy may increase the risk of type 1 diabetes in the offspring. our aim was to evaluate the role of first trimester enterovirus infections in a larger cohort of pregnant women. two series of pregnant women were analyzed as follows: 948 women (series 1) and 680 women (series 2) whose child developed clinical diabetes before the ages of 15 or 7 years, respectively. an equal number of control women with a nondiabetic child was ... | 2002 | 12145172 |
| transient broca aphasia in an elderly man caused by coxsackievirus b5. | | 2002 | 12242564 |
| comparative inactivation of enteroviruses and adenovirus 2 by uv light. | the doses of uv irradiation necessary to inactivate selected enteric viruses on the u.s. environmental protection agency contaminant candidate list were determined. three-log reductions of echovirus 1, echovirus 11, coxsackievirus b3, coxsackievirus b5, poliovirus 1, and human adenovirus type 2 were effected by doses of 25, 20.5, 24.5, 27, 23, and 119 mw/cm(2), respectively. human adenovirus type 2 is the most uv light-resistant enteric virus reported to date. | 2002 | 12324370 |
| a season of aseptic meningitis in germany: epidemiologic, clinical and diagnostic aspects. | we assessed epidemiologic, clinical and laboratory features of aseptic meningitis during one season of multiserotype enteroviral meningitis in east germany in 70 consecutive patients with aseptic meningitis admitted to the children's university hospital leipzig. | 2002 | 12488662 |
| crystal structure of swine vesicular disease virus and implications for host adaptation. | swine vesicular disease virus (svdv) is an enterovirus of the family picornaviridae that causes symptoms indistinguishable from those of foot-and-mouth disease virus. phylogenetic studies suggest that it is a recently evolved genetic sublineage of the important human pathogen coxsackievirus b5 (cbv5), and in agreement with this, it has been shown to utilize the coxsackie and adenovirus receptor (car) for cell entry. the 3.0-a crystal structure of strain uk/27/72 svdv (highly virulent) reveals th ... | 2003 | 12692248 |
| molecular typing and epidemiology of enteroviruses identified from an outbreak of aseptic meningitis in belgium during the summer of 2000. | non-polio enteroviruses are the most common cause of aseptic meningitis worldwide. from may to september 2000, a major outbreak of aseptic meningitis occurred in belgium. cerebrospinal fluid samples (csf) of 122 patients were found to contain enterovirus rna using diagnostic rt-pcr that targeted a 231-bp gene fragment in the 5' noncoding region. in addition, a molecular typing method was developed based on rt-nested pcr and sequencing directly from csf(a) 358-bp fragment in the aminoterminal par ... | 2003 | 12767006 |
| an epidemic of aseptic meningitis due to coxsackievirus b5 in nara prefecture, japan: an epidemiological analysis by pcr-rflp. | | 2003 | 12824693 |
| human rhabdomyosarcoma cells for rapid detection of enteroviruses by shell-vial assay. | the ability of the rd (rhabdomyosarcoma) and mrc-5 cell-lines to detect enteroviruses in 33 clinical samples (cerebrospinal fluid, stools and throat swabs) was evaluated. the samples had previously tested enterovirus-positive by traditional tube-culture and had been frozen after their initial processing. by traditional tube-culture, 100 and 85 % of samples were positive for enterovirus in rd and mrc-5 cells, respectively. by rapid shell-vial assay, 94 and 45.5 % were positive after 48 h incubati ... | 2003 | 12909656 |
| structure of swine vesicular disease virus: mapping of changes occurring during adaptation of human coxsackie b5 virus to infect swine. | the structure of swine vesicular disease virus (svdv) was solved and refined at a 3.0-a resolution by x-ray crystallography to gain information about the role of sequence changes that occurred as this virus evolved from the parental human pathogen coxsackievirus b5 (cvb5). these amino acid substitutions can be clustered in five distinct regions: (i) the antigenic sites, (ii) the hydrophobic pocket of the vp1 beta-sandwich, (iii) the putative car binding site, (iv) the putative heparan sulfate bi ... | 2003 | 12941886 |
| assessment of an enterovirus sewage surveillance system by comparison of clinical isolates with sewage isolates from milwaukee, wisconsin, collected august 1994 to december 2002. | the quantity and serotypes of enteroviruses (evs) in the influent of a local sewage treatment plant were compared to local clinical ev cases to determine if testing of sewage is adequate for an ev surveillance system. the study was carried out from august 1994 to december 2002. monthly influent specimens were processed by organic flocculation, and dilutions of concentrate were inoculated onto a number of different cell types for virus isolation. evs were detected in 88 of 100 monthly influent sa ... | 2003 | 14660364 |
| molecular characterization of coxsackievirus b5 isolates. | coxsackie b viruses of serotype 5 are associated frequently with sporadic cases of neurological diseases, epidemics of meningitis, and chronic diseases such as cardiomyopathy and diabetes. in this article, 15 strains of coxsackievirus b5 isolated from patients with neurological disorders and healthy people were investigated by partial sequencing in the 5' half of the vp1 region and compared to other published sequences of coxsackievirus b5, in the same genomic region. all coxsackievirus b5 seque ... | 2004 | 14695669 |
| mechanisms of beta cell death during restricted and unrestricted enterovirus infection. | coxsackie b virus (cvb-5) infections potentially trigger and accelerate pancreatic beta cell damage leading to type 1 diabetes. in vivo, all viruses face natural resistance mediated by various host factors which restrict the progression of infection. thus, the aims of this study were to generate a tissue culture model of restricted coxsackie b virus infection in primary islet cells by preventing the production of viral progeny with a selective inhibitor of viral rna replication and to investigat ... | 2004 | 14748069 |
| novel 1,2,5-oxadiazine derivatives--synthesis and in vitro biological studies. | a new synthetic approach to the 1,2,5-oxadiazine ring system is described. 2-substituted or 2,4-disubstituted 2h-1,2,5-oxadiazine-3,6(4h,5h)-dione derivatives 4 were prepared by cyclisation of hydroxamic acids 3 derived from n-(1-benzotriazolylcarbonyl)-amino acids 1. the structures of the synthesised compounds were fully characterised by ir, 1h and 13c nmr spectroscopy and elemental analysis. the aim of this study was to evaluate biological activity of the newly synthesised oxadiazine derivativ ... | 2003 | 14769241 |
| mechanisms of coxsackievirus b5 mediated beta-cell death depend on the multiplicity of infection. | coxsackievirus infections may trigger and accelerate pancreatic beta-cell death, leading to type i diabetes. unrestricted coxsackievirus b5 replication in cultured beta-cells inoculated with high multiplicity leads to rapid lytic cell death. evidence from other virus-host cell systems indicates that host cell responses to infection may depend on the multiplicity of infection (moi). thus, the aim of this study was to compare the mechanisms of beta-cell death during high versus low multiplicity of ... | 2004 | 14981761 |
| inactivation of selected picornaviruses by high hydrostatic pressure. | the potential of high hydrostatic pressure processing (hpp) to inactivate aichi virus (aiv), human parechovirus-1 (hpev-1), and the coxsackievirus strains a9 and b5 was investigated. for coxsackievirus a9 (cav9), a 5-min hpp treatment in minimum essential growth medium (mem) supplemented with 10% fetal bovine sera (fbs) resulted in 3.4-, 6.5-, and 7.6-log(10) tissue culture infectious dose (50%) (tcid(50)) reductions at 400, 500, and 600megapascals (mpa), respectively. for hpev-1, a 5-min treatm ... | 2004 | 15084405 |
| buthionine sulfoximine inhibits cytopathic effect and apoptosis induced by infection with human echovirus 9. | we studied the effect of buthionine sulfoximine (bso) on the replication of an isolate of human echovirus 9 (ev9) and the apoptosis induced by it in gmk cells. one hundred microm bso markedly inhibited the cytopathic effect (cpe) induced by ev9. bso also significantly inhibited apoptosis induced by ev9. bso did not influence replication of ev9 genome, but inhibited virion formation. these results suggest that the inhibition by bso of cpe and apoptosis induced by ev9 may be associated with the im ... | 2004 | 15168199 |
| coxsackie b viruses use multiple receptors to infect human cardiac cells. | viral myocarditis is an inflammatory disease of the heart muscle that can be fatal. the primary viruses that have been linked to myocarditis and dilated cardiomyopathy are the human enteroviruses. the most common viruses associated with this disease are the coxsackie b viruses and in particular coxsackievirus b3 and coxsackievirus b5. early events in viral infection include attachment of the virus onto cell surface receptors. even though, cd55 and coxsackievirus adenovirus receptor protein (car) ... | 2004 | 15332279 |
| neonatal meningitis caused by coxsackievirus b5. | enteroviral infections are more severe in neonates than in older children, and coxsackievirus b is most frequently associated with severe neonatal disease. we summarize the course and clinical data of four neonates with meningitis caused by coxsackievirus b5. their clinical symptoms and signs were persistent fever, poor appetite, and decreased activity, with vomiting in two. laboratory analysis of cerebrospinal fluid revealed the following: white blood count, 340-660/mm3; red blood count, 0-250/ ... | 2004 | 15624370 |
| phenotypic and genetic changes in coxsackievirus b5 following repeated passage in mouse pancreas in vivo. | common enterovirus infections appear to initiate or facilitate the pathogenetic processes leading to type 1 diabetes, and also sometimes precipitate the clinical disease. in experimental infection of mice, coxsackieviruses have shown to have a strong affinity for the exocrine tissue, while even in lethal cases, the islets remain unaffected. the virus strain most intensively studied in this respect is the diabetogenic variant e2 of coxsackievirus b4. in addition, it is known that all six serotype ... | 2005 | 15714484 |
| distribution of viral rna in mouse tissues during acute phase of coxsackievirus b5 infection. | to investigate histopathological changes and distribution of coxsackievirus b5 (cvb5) rna in mouse heart, liver, and pancreas during the acute phase of infection. | 2005 | 15812189 |
| simultaneous type 1 diabetes onset in mother and son coincident with an enteroviral infection. | enterovirus (ev) infections have been implicated in the development of type 1 diabetes. (t1d). they may cause beta-cell destruction either by cytolytic infection of the cells or indirectly by triggering the autoimmune response. virus was isolated from a woman at diagnosis of t1d (tuvemo 1) and in addition, virus was isolated from her son at diagnosis of t1d at the same day (tuvemo 2). none of the isolates could initially be serotyped by conventional methods. the tuvemo 1 virus was genotyped and ... | 2005 | 15911432 |
| global profiling of coxsackievirus- and cytokine-induced gene expression in human pancreatic islets. | it is thought that enterovirus infections initiate or facilitate the pathogenetic processes leading to type 1 diabetes. exposure of cultured human islets to cytolytic enterovirus strains kills beta cells after a protracted period, suggesting a role for secondary virus-induced factors such as cytokines. | 2005 | 15991020 |
| [investigation of enterovirus in cases of aseptic meningitis syndrome of belém, pa]. | with the objective of isolating and identifying enteroviral serotypes, which are the most frequent etiologic agents of aseptic meningitis syndrome, cerebrospinal fluid samples were studied from patients referred to the health unit in the city of belém (state of pará, brazil), from march 2002 to march 2003. the samples were inoculated in rd and hep-2 cells line, and the positive were identified by neutralization test or indirect immunofluorescence. out of 249 samples, 33 (13.2%) were positive 57. ... | 2005 | 16172753 |
| sequence analysis of the 5' untranslated region of swine vesicular disease virus reveals block deletions between the end of the internal ribosomal entry site and the initiation codon. | swine vesicular disease virus (svdv) is a picornavirus closely related to the human pathogen coxsackievirus b5. in common with other picornaviruses, the 5' untranslated region (5' utr) of svdv contains an internal ribosomal entry site (ires) that plays an important role in cap-independent translation. the aim of this study was to use rt-pcr and sequencing to characterize a fragment of the 5' utr encompassing the entire ires. sequence analysis demonstrated high nucleotide identities within the ir ... | 2005 | 16186229 |
| annual report of the australian national poliovirus reference laboratory, 2004. | the australian national poliovirus reference laboratory at the victorian infectious diseases reference laboratory is the world health organization designated laboratory for the isolation and testing of poliovirus from clinical specimens within australia, the pacific island countries and brunei darussalam. surveillance for acute flaccid paralysis (afp) within australia, the main clinical manifestation of poliomyelitis, is also coordinated at the victorian infectious diseases reference laboratory ... | 2005 | 16220861 |
| enterovirus meningitis in brazil, 1998-2003. | acute viral infections of the central nervous system (cns) such as acute flaccid paralysis, meningitis, and encephalitis, are responsible for a high morbidity, particularly in children. non-polio enteroviruses (npev) are known to be responsible for over 80% of viral meningitis in which the etiologic agent is identified. in the present study, we show the frequency of enterovirus meningitis in brazil from december 1998 to december 2003. enterovirus were isolated from 162 (15.8%), of a total of 1,0 ... | 2006 | 16299728 |
| congenital coxsackievirus b5 infection: report of one case. | in infants and children, enterovirus (ev) infections are very common. in neonates, ev infections may lead to fatal myocarditis, encephalitis, or necrotizing hepatitis. transplacental viremia before delivery is possible but has only been demonstrated occasionally. the presence or absence of passively acquired maternal antibody specific for the infecting ev serotype plays the most important role in neonatal ev infections. we report a fatal case of congenital coxsackievirus b5 infection, confirmed ... | 2005 | 16640011 |
| [etiology of aseptic meningitis prevalent in xuzhou]. | to find the pathogenic agents of aseptic meningitis prevalent in xuzhou of jiangsu province in 2001. | 2006 | 16642223 |
| enteroviral infection outbreak in the republic of belarus: principal characteristics and phylogenetic analysis of etiological agents. | for the last decade enterovirus outbreaks were registered in all of six districts of belarus. two of them, reported in 1997 (in gomel) and in 2003 (in minsk), were the most extensive and involved 461 and 1,351 patients respectively. virus echo 30 was identified as the dominant etiologic agent of the outbreak in 1997 whereas co-circulation of echo 30, echo 6 and coxsackievirus b5 took place in 2003. analysis of clinical manifestations during the minsk outbreak revealed unusually high rate of seve ... | 2006 | 16830607 |