| the structure and molecular weight of satellite tobacco necrosis virus: a neutron small-angle scattering study. | | 1977 | 929991 |
| subgenomic rnas mediate expression of cistrons located internally on the genomic rna of tobacco necrosis virus strain a. | upon infection of tobacco protoplasts, the genomic rna of tobacco necrosis virus strain a (tnv-a) accumulates linearly in time. the accumulation patterns of the two subgenomic rnas resemble those of endogenous mrnas in that the peak levels are reached after several hours. the accumulation of the 1.3-kb subgenomic rna is delayed by 1 h compared with that of the 1.6-kb subgenomic rna, which illustrates the important role of the subgenomic rnas in the regulation of tnv-a gene expression. the locati ... | 1992 | 1404597 |
| the complete nucleotide sequence of tobacco necrosis virus strain d. | the complete sequence of the rna genome of tobacco necrosis virus strain d (tnv-d) consisting of 3759 nucleotides has been determined. the positive strand contains five open reading frames (orfs). the 5'-proximal orf encodes a 22k protein terminating with an amber codon which may be read through to produce a 82k protein (p82). two small centrally located orfs each encode two out-of-frame 7k proteins (p7a and p7b). the 3'-proximal orf encodes the 29k coat protein (cp), the n terminus of which has ... | 1991 | 1856691 |
| genome structure of tobacco necrosis virus strain a. | an almost complete sequence of the rna genome of tobacco necrosis virus (tnv) strain a has been determined. the genome organization is very similar to that of carnation mottle virus (carmv) and turnip crinkle virus (tcv). the 5'-proximal open reading frame (orf) encodes a 23-kda protein and read-through of its amber codon into the second orf is presumably used for the translation of a 82-kda protein. the third large orf encodes the 30-kda coat protein. two small orfs are located upstream and one ... | 1990 | 2371773 |
| molecular cloning and sequencing of the coat protein gene of a nebraskan isolate of tobacco necrosis virus: the deduced coat protein sequence has only moderate homology with those of strain a and strain d. | a partial nucleotide sequence spanning the coat protein (cp) gene of a nebraskan isolate of tobacco necrosis virus (tnv-ne) has been determined. the sequence contains at least four open reading frames (orfs). the 5'-terminal orf encodes a protein that has 86% and 38% homology with the polymerases of strains a (tnv-a) and d (tnv-d), respectively. the second and third orfs probably encode 10.7 kda and 6.2 kda proteins (p 10.7 and p 6.2). these are respectively 90% and 96% amino acid homologous enc ... | 1993 | 8379851 |
| specificity of satellite activation by tobacco necrosis virus correlates with nucleic acid hybridization pattern between helper virus isolates. | tobacco necrosis virus (tnv) comprises over 20 different isolates which are usually classified on the basis of serological cross-reactivity of their virus particles or specific activation of satellite virus strains (stnv-1, -2, and -c). we have studied the relationships between five tnv isolates, tnv-a, -g, -cn, -d, and -ac36 which exhibit considerable differences in symptom formation on phaseolus vulgaris. it is shown that, like tnv-a, tnv-g and -cn support the multiplication of stnv-1 and -2. ... | 1993 | 8460499 |
| nucleotide sequence of the genome of a citrus isolate of olive latent virus 1. | the 3699 nt genome of olive latent virus 1 (olv-1), described years ago from southern italy as a putative sobemovirus, was completely sequenced. olv-1 genomic rna was not polyadenylated and had a structure virtually identical to that of species of the necrovirus rather than the sobemovirus genus. five open reading frames (orfs) were identified, of which the 5'-proximal encoded a 23k protein and ended with an amber codon whose readthrough could yield a putative 82k product. this polypeptide had e ... | 1996 | 8678829 |
| the satellite tobacco necrosis virus: a single protein and its genetic code. | | 1964 | 14224378 |
| conservation of rna structures enables tnv and bydv 5' and 3' elements to cooperate synergistically in cap-independent translation. | the subgenomic rna 2 of tobacco necrosis virus a (tnv sgrna2) encodes the viral coat protein, is unpolyadenylated and presumably uncapped. here, we show that tnv sgrna2 is translated cap independently. this cap-independent translation requires the leader and a 140 nt element of the trailer both in wheat germ extract and in tobacco protoplasts. similar to barley yellow dwarf virus (bydv), the tnv 5' and 3' elements stimulate translation synergistically. computer-aided phylogenetic analysis of the ... | 2004 | 15020708 |
| complete nucleotide sequence of a new strain of tobacco necrosis virus a infecting soybean in china and infectivity of its full-length cdna clone. | the complete nucleotide sequence of a virus isolated from soybean (glycine max (l.) merr.) in china, previously identified as a new strain of tobacco necrosis virus a (tnv-a) based on its biological, serological properties, and coat protein (cp) sequence and named as tnv-a c, was determined and compared with that of tnv-a and other closely related necroviruses and carmoviruses. the viral rna genome consists of 3,682 nucleotides and contains five open reading frames (orfs). tnv-a c showed 86.4% o ... | 2008 | 18071890 |
| development of tobacco necrosis virus a as a vector for efficient and stable expression of fmdv vp1 peptides. | plant virus-based expression systems provide attractive alternatives for production of animal virus-originated antigenic peptides. in the present study, an infectious cdna clone of tobacco necrosis virus a chinese isolate (tnv-a(c)) was used for expression of different peptides derived from foot and mouth disease virus (fmdv) serotype o vp1 fused downstream of the coat protein (cp) open reading frame (orf). chenopodium amaranticolor inoculated with in vitro transcripts of the chimaeras developed ... | 2010 | 20331532 |
| molecular characterization of a hungarian isolate of tobacco necrosis virus a. | | 2010 | 20364277 |
| validation of reference genes for gene expression studies in virus-infected nicotiana benthamiana using quantitative real-time pcr. | nicotiana benthamiana is the most widely-used experimental host in plant virology. the recent release of the draft genome sequence for n. benthamiana consolidates its role as a model for plant-pathogen interactions. quantitative real-time pcr (qpcr) is commonly employed for quantitative gene expression analysis. for valid qpcr analysis, accurate normalisation of gene expression against an appropriate internal control is required. yet there has been little systematic investigation of reference ge ... | 2012 | 23029521 |
| a degenerate pair of primers for simultaneous detection of four alpha- and betanecroviruses. | the high infection levels due to olive latent virus 1 (olv-1), olive mild mosaic virus (ommv) (alphanecrovirus) and tobacco necrosis virus d (tnv-d) (betanecrovirus) in portuguese olive orchards prompted us to develop a rapid pcr-based assay for the simultaneous detection of these viruses aimed at the sanitary selection and marketing of plant material in compliance with european union regulations. a pair of degenerate oligonucleotide primers, parrdrp5' and parcoat3' was designed based on conserv ... | 2014 | 25116202 |
| identification of an internal rna element essential for replication and translational enhancement of tobacco necrosis virus a(c). | different regulatory elements function are involved in plant virus gene expression and replication by long-distance rna-rna interactions. a cap-independent functional element of the barley yellow dwarf virus (bydv) - like translational enhancer (bte) is present in tobacco necrosis virus a (tnv-a), a necrovirus member in the tombusviridae family. in this paper, an rna stretch flanking the 5' proximal end of the tnv-a(c) coat protein (cp) gene was shown to be essential for viral replication in che ... | 2013 | 23460916 |