| sugarcane yellow leaf virus: a novel member of the luteoviridae that probably arose by inter-species recombination. | the 5895 nucleotide long single-stranded rna genome of sugarcane yellow leaf virus florida isolate (scylv-f) includes six major orfs. all but the first of these are homologous to genes of known function encoded by viruses of the three newly defined genera in the luteoviridae: ('luteovirids'), i.e. poleroviruses, luccccteoviruses and the enamoviruses. scylv-f orfs 1 and 2 are most closely related to their polerovirus counterparts, whereas scylv-f orfs 3 and 4 are most closely related to counterpa ... | 2000 | 10859394 |
| sugarcane yellow leaf virus: an emerging virus that has evolved by recombination between luteoviral and poleroviral ancestors. | we have derived the genomic nucleotide sequence of an emerging virus, the sugarcane yellow leaf virus (scylv), and shown that it produces one to two subgenomic rnas. the family luteoviridae currently includes the luteovirus, polerovirus, and enamovirus genera. with the new scylv nucleotide sequence and existing luteoviridae sequence information, we have utilized new phylogenetic and evolutionary methodologies to identify homologous regions of luteoviridae genomes, which have statistically signif ... | 2000 | 10725208 |
| nucleotide sequence and e. coli expression of the coat protein gene of the yellowing strain of soybean dwarf luteovirus. | the nucleotide sequence of the coat protein gene of the yellowing (y) strain of soybean dwarf virus (sbdv) was determined from cloned cdna. the gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated m(r) of 22,200. the identity of the open reading frame (orf) encoding the coat protein was confirmed by expression of an escherichia coli beta-galactosidase fusion protein and detection by a dot blot immunoassay. sequence comparisons of the deduced coat protein amino ... | 1993 | 8240014 |
| relationships among luteoviruses based on nucleic acid hybridization and serological studies. | the luteoviruses barley yellow dwarf virus (bydv-pav and bydv-rpv), bean leaf roll virus (blrv) beet western yellows virus (bwyv), carrot red leaf virus, potato leaf roll virus, and soybean dwarf virus (sdv) were compared by hybridization with random cdna probes and serologically with polyclonal antisera. for hybridizations, filters had each rna blotted in duplicate dots at 10 ng/dot. random-primed cdna probes were prepared from 300 ng of each rna and used to probe filters at three levels of str ... | 1990 | 2338398 |
| influence of elisa conditions on detection of serological relationships among luteoviruses. | three variations in the elisa procedure were used in an attempt to understand the basis for serological relationships among three isolates of barley yellow dwarf virus (bydv-rpv-i from illinois, bydv-rpv-n from new york and bydv-pav from illinois), beet western yellows virus (bwyv) and soybean dwarf virus (sdv). detection of serological relationships was dependent on the state of the virus particle (e.g. dissociated or intact) and the method of detection (e.g. direct or indirect). in indirect el ... | 1991 | 1713915 |
| host adaptation of soybean dwarf virus following serial passages on pea (pisum sativum) and soybean (glycine max). | soybean dwarf virus (sbdv) is an important plant pathogen, causing economic losses in soybean. in north america, indigenous strains of sbdv mainly infect clover, with occasional outbreaks in soybean. to evaluate the risk of a us clover strain of sbdv adapting to other plant hosts, the clover isolate sbdv-md6 was serially transmitted to pea and soybean by aphid vectors. sequence analysis of sbdv-md6 from pea and soybean passages identified 11 non-synonymous mutations in soybean, and six mutations ... | 2017 | 28635666 |
| [codon bias and nucleotide substitutions in soybean dwarf virus]. | computational analysis of codon usage bias and spontaneous nucleotide substitutions in six strains of soybean dwarf virus was performed. it was shown that synonymous codon usage in the virus genes varies widely depending on the gene, gene overlapping, codon, codon's two first nucleotides, mononucleotide context located upstream and downstream of the codon, gc-content in virus-encoded genes and in the third codon position. overlapping of genes causes a 2.5-fold decrease of the total number of nuc ... | 2012 | 22830203 |
| fine mapping of foxglove aphid (aulacorthum solani) resistance gene raso1 in soybean and its effect on tolerance to soybean dwarf virus transmitted by foxglove aphid. | soybean dwarf virus (sbdv) causes serious dwarfing, yellowing and sterility in soybean (glycine max). the soybean cv. adams is tolerant to sbdv infection in the field and exhibits antibiosis to foxglove aphid (aulacorthum solani), which transmits sbdv. this antibiosis (termed "aphid resistance") is required for tolerance to sbdv in the field in segregated progenies of adams. a major quantitative trait locus, raso1, is reported for foxglove aphid resistance. our objectives were to fine map raso1 ... | 2012 | 23136500 |
| development and validation of dna markers linked to sdvy-1, a common bean gene conferring resistance to the yellowing strain of soybean dwarf virus. | the yellowing strain of soybean dwarf virus (sbdv-ys) causes yellowing and yield loss in common bean (phaseolus vulgaris). the most effective control is achieved through breeding for resistance. an indeterminate climbing cultivar with a white seed coat, 'oofuku', is resistant to sbdv-ys in inoculation tests. we crossed 'oofuku' with an elite cultivar, 'taisho-kintoki', which is sbdv-ys-susceptible, determinate dwarf with a red-purple seed coat, and performed amplified-fragment-length polymorphis ... | 2014 | 25914596 |
| control of virus diseases in soybeans. | soybean, one of the world's most important sources of animal feed and vegetable oil, can be infected by numerous viruses. however, only a small number of the viruses that can potentially infect soybean are considered as major economic problems to soybean production. therefore, we consider management options available to control diseases caused by eight viruses that cause, or have the potential to cause, significant economic loss to producers. we summarize management tactics in use and suggest di ... | 2014 | 25410106 |
| fine mapping of the major soybean dwarf virus resistance gene rsdv1 of the soybean cultivar 'wilis'. | soybean dwarf virus (sbdv), a luteoviridae family member, causes dwarfing, yellowing and sterility of soybean (glycine max), leading to one of the most serious problems in soybean production in northern japan. previous studies revealed that the indonesian soybean cultivar 'wilis' is resistant to sbdv and that the resistance can be introduced into japanese cultivars. a major qtl for sbdv resistance has been reported between ssr markers sat_217 and satt211 on chromosome 5. in this study, we named ... | 2013 | 24399914 |
| molecular analysis of soybean dwarf virus isolates in the eastern united states confirms the presence of both d and y strains and provides evidence of mixed infections and recombination. | soybean dwarf virus (sbdv), first identified as an agricultural problem in japan, has emerged as a growing problem in the midwestern united states. the majority of research on sbdv had been limited to four lab maintained strains from japan. sbdv had been found in clover in the eastern united states, but these isolates rarely emerged into soybeans. these isolates were analyzed by multiplex pcr and sequencing, revealing that some were infections of both y and d components, including a recombinant ... | 2011 | 21256532 |
| detection and discrimination of members of the family luteoviridae by real-time pcr and sybr® greener™ melting curve analysis. | this study investigated the suitability of a two step real-time rt-pcr melting curve analysis as a tool for the detection and discrimination of nine species in the plant virus family luteoviridae, being soybean dwarf virus [sbdv], bean leafroll virus [blrv], beet chlorosis virus [bchv], beet mild yellowing virus [bmyv], beet western yellows virus [bwyv], cereal yellow dwarf virus-rpv [cydv-rpv], cucurbit aphid-borne yellows virus [cabyv], potato leafroll virus [plrv] and turnip yellows virus [tu ... | 2010 | 20933015 |
| sequence diversity of readthrough proteins of soybean dwarf virus isolates from the midwestern united states. | the amino acid sequence diversity of readthrough proteins (rtps) of 24 dwarfing isolates of soybean dwarf virus (sbdv) from wisconsin and illinois was analyzed. the rtp, a minor component of viral capsids, has a significant role in specificity of aphid transmission of luteovirids. among the isolates, nucleotide sequence identities ranged from 95 to 100%. the predicted amino acid sequences differed at 56 amino acid positions in the 54 kda rtd compared to only five positions in the 22 kda cp. phyl ... | 2009 | 19360376 |
| epidemiology and integrated management of persistently transmitted aphid-borne viruses of legume and cereal crops in west asia and north africa. | cool-season food legumes (faba bean, lentil, chickpea and pea) and cereals (bread and durum wheat and barley) are the most important and widely cultivated crops in west asia and north africa (wana), where they are the main source of carbohydrates and protein for the majority of the population. persistently transmitted aphid-borne viruses pose a significant limitation to legume and cereal production worldwide. surveys conducted in many countries in wana during the last three decades established t ... | 2009 | 19152820 |
| identification, characterization, and relatedness of luteovirus isolates from forage legumes. | abstract virus isolates from forage legumes collected from eight different states were identified as luteoviruses closely related to soybean dwarf luteovirus dwarfing (sbdv-d) and yellowing (sbdv-y) described in japan. all isolates produced reddened leaf margins in subterranean clover and were transmitted in a persistent manner by acrythosiphon pisum, but not by aulacorthum solani. specific monoclonal antibodies raised against sbdv-y were differentially reactive with endemic isolates. immunoblot ... | 1999 | 18944749 |
| the n-terminal region of the readthrough domain is closely related to aphid vector specificity of soybean dwarf virus. | abstract it has been speculated that the n-terminal half of the readthrough domain (rtd) encoded by open reading frame 5 of soybean dwarf virus (sbdv) is related to the vector specificity. to further investigate this hypothesis, transmissibility via aphids was tested on 17 sbdv isolates and comparisons of the deduced amino acid sequences of the coat protein (cp) and other proteins encoded by the rtd were made between these isolates. isolates were distinguished into four strains: ys, causing yell ... | 2003 | 18943620 |
| soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene. | we transformed a construct containing the sense coat protein (cp) gene of soybean dwarf virus (sbdv) into soybean somatic embryos via microprojectile bombardment to acquire sbdv-resistant soybean plants. six independent t(0) plants were obtained. one of these transgenic lines was subjected to further extensive analysis. three different insertion patterns of southern blot hybridization analysis in t(1) plants suggested that these insertions introduced in t(0) plants were segregated from each othe ... | 2007 | 17632723 |
| biolistic inoculation of soybean plants with soybean dwarf virus. | soybean dwarf virus (sbdv), a member of the genus luteovirus, has been transmitted only by aphid vectors. the possibility of using a biolistic procedure of transmitting sbdv to soybean plants without relying on aphid vectors was investigated. biolistic inoculation using the helios gene gun system with rna transcribed in vitro from a full-length cdna clone of the ds strain of sbdv (psv-ds) resulted in 1/12 to 3/13 soybean plants infected systemically. the infected soybean plants showed the charac ... | 2006 | 16837069 |
| development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus. | in an attempt to generate soybean plants resistant to soybean dwarf virus (sbdv), we transformed a construct containing inverted repeat-sbdv coat protein (cp) genes spaced by beta-glucuronidase (gus) sequences into soybean somatic embryos via microprojectile bombardment. three t(0) plants with an introduced cp gene were obtained, and one generated t(1) seeds. the presence of the transgene in t(1) plants was confirmed by pcr and southern blot hybridization analysis, but expression of cp was not d ... | 2006 | 16763847 |
| discrimination of four soybean dwarf virus strains by dot-blot hybridization with specific probes. | soybean dwarf virus (sbdv) is divided into four strains (ys, yp, ds, and dp) on the basis of host symptoms in infected soybean plants and on aphid vector specificity. to detect and discriminate each strain of sbdv by dot-blot hybridization, probes y, d, s, and p were prepared. probes y and d, covering most of the 3'-noncoding region of the viral genome containing the sequence of small subgenomic rna, could discriminate strains in accord with the host symptoms. probes s and p were derived from th ... | 2006 | 16325927 |
| characterization of the small subgenomic rna of soybean dwarf virus. | we have characterized a small subgenomic rna of japanese strains of soybean dwarf virus (sbdv). northern blot analyses of sbdv-infected plants showed that the small rna contained the 3' terminal sequence of the genome and was detected in four typical japanese sbdv strains, ys, yp, ds and dp. in the case of sbdv-ds, the rna was 220 nucleotides in length and was transcribed from the 3' terminal region of the genome. this rna appeared at a similar time to genomic rna and a large sgrna, and thereaft ... | 2003 | 14505093 |
| nucleotide sequence shows that bean leafroll virus has a luteovirus-like genome organization. | the complete nucleotide sequence of the bean leafroll virus (blrv) genomic rna and the termini of its smallest subgenomic rnas were determined to better understand its mechanisms of gene expression and replication and its phylogenetic position within the luteoviridae: the number and placement of open reading frames (orfs) within the blrv genome was luteovirus-like. the nucleotide and predicted amino acid sequences of blrv were most similar to those of soybean dwarf virus (sbdv). phylogenetic ana ... | 2002 | 12075101 |
| comparison of complete nucleotide sequences of genomic rnas of four soybean dwarf virus strains that differ in their vector specificity and symptom production. | soybean dwarf virus (sbdv) is divided into four strains, namely ys, yp, ds and dp. ys and yp cause yellowing in soybeans, while ds and dp cause dwarfing. ys and ds are transmitted by aulacorthum solani, while yp and dp are transmitted by acyrthosiphon pisum. to clarify the taxonomic relationship between the four strains and to classify sbdv into an appropriate genus in the luteoviridae, we determined the complete nucleotide sequences of genomic rnas of four isolates belonging to each of the stra ... | 2001 | 11722012 |
| molecular evidence that sugarcane yellow leaf virus (scylv) is a member of the luteoviridae family. | a previously uncharacterized virus was reported in southeast brazil causing a yellowing leaf disease in sugarcane. the virus, termed sugarcane yellow leaf virus (scylv), shares features typical of the luteoviruses. to start the molecular characterization of scylv, the nucleotide sequence of the coat protein (cp), 17 kda protein and c-terminus of the rna-dependent rna polymerase coding regions was determined from an rt-pcr amplification product. comparisons showed that the deduced amino acid sequ ... | 2000 | 10881686 |
| sequence variability in the genome-3'-terminal region of bydv for 10 geographically distinct pav-like isolates of barley yellow dwarf virus: analysis of the orf6 variation. | nucleotide sequence of the genome terminal region 3' to the capsid-readthrough cistron were compared for 10 pav-like isolates of barley yellow dwarf virus (bydv) from three continents. the sequenced region varied in length from 853 to 864 nucleotides and the extent of sequence homology among the isolates ranged from 84 to 99%. sequence variations occur mainly in two locations, one in the orf6 coding region and the other near the genome 3' terminus. sequence homology grouping reveals three geneti ... | 1994 | 7832645 |