| [potato leafroll virus: purification, serologic detection and quantitative analysis in plants]. | the potato leafroll virus has been purified from potato. an antiserum obtained allows the use of the enzyme linked-immunosorbent assay, a direct means to detect the plrv in the plant and to assess its real concentration. | 1978 | 96980 |
| rna in potato leafroll virus. | | 1979 | 446731 |
| potato leafroll virus contains a double-stranded dna. | | 1976 | 1266041 |
| analysis of epitopes on potato leafroll virus capsid protein. | pepscan hexapeptides prepared to the capsid protein amino acid sequence of potato leafroll luteovirus (plrv) were tested against both polyclonal and monoclonal antibodies. twelve continuous epitopes were identified: 11 were detected by two different plrv polyclonal antisera, but only 4 were detected by both antisera. the 12th epitope reacted with monoclonal antibody bg3. the location of most of the epitopes did not correlate well with antigenic areas predicted by computer algorithms. comparison ... | 1992 | 1384231 |
| resistance to phloem transport of potato leafroll virus in potato plants. | a 'double-graft sandwich' technique in which sections of potato stem from different potato cultivars were grafted between a susceptible healthy stock plant and a potato leafroll virus (plrv)-infected scion was used to study the rate of phloem transport of plrv in cultivars differing in resistance to plrv infection (ir) and accumulation (ar). resistance to phloem transport (i.e. delayed plrv systemic movement) was found in bismark cultivar (ir a(s)). this was independent of ir and ar as the rate ... | 1992 | 1469360 |
| use of group-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses. | a general diagnostic assay for a number of distinct luteoviruses was developed using the polymerase chain reaction (pcr) and restriction enzyme analysis. two minimally degenerate, group-specific primers were derived from previously published rna sequences of three luteoviruses. this primer pair generated specific pcr fragments of about 530 bp from extracts of plants infected with potato leafroll virus, beet western yellows virus, or new york barley yellow dwarf virus (bydv) serotypes mav, pav, r ... | 1991 | 1675249 |
| a characterization of epitopes on potato leafroll virus coat protein. | a panel of ten stable hybridoma cell lines secreting monoclonal antibodies (mabs) specific for potato leafroll virus (plrv) antigen, was produced in two fusion experiments with murine splenic and myeloma cells. using different elisa procedures and western blotting it was shown that one mab detected a continuous epitope and nine mabs reacted with conformation-dependent ones. the conformation-dependent epitopes could be separated into two groups after alkaline treatment of the virions. the mabs we ... | 1990 | 1701986 |
| the 5'-terminal sequence of potato leafroll virus rna: evidence of recombination between virus and host rna. | the discrepancy between published sequences of the 5' non-coding regions of rna of a scottish (s) and that of dutch (d), australian and canadian isolates of potato leafroll virus (plrv) was investigated. reverse transcription followed by amplification by polymerase chain reaction showed that rna from three distinct scottish isolates of plrv contained molecules with 5' ends like that of the original scottish isolate. however, determination of the 5'-terminal sequences of rna in two of these prepa ... | 1991 | 1717645 |
| visualization of a luteovirus in the vector aphid's body by two gold immunolabelling techniques: a comparative study. | two gold immunolabelling techniques using electron microscopy were compared to examine the in situ localization of a luteovirus, potato leafroll virus (plrv), inside its main aphid vector, myzus persicae sulz. with gildow's technique, virus particles were labelled prior to fixation, embedding by injecting plrv-specific iggs into the living aphids. this facilitated the detection of extracellular particles located between the basal lamina and plasmalemma by trapping them in aggregates. the heavy c ... | 1991 | 1800527 |
| nucleotide sequence analysis and genomic organization of the ny-rpv isolate of barley yellow dwarf virus. | cdna clones representing the ssrna genome of the ny-rpv isolate of barley yellow dwarf luteovirus (bydv) were sequenced and 5600 nucleotides of the genome were determined. the deduced genome organization has limited similarity to that of another bydv isolate, vic-pav, but is identical to that of beet western yellows (bwyv) and potato leafroll (plrv) luteoviruses. ny-rpv has six major positive-sense open reading frames (orfs) and, by comparison with rna-dependent rna polymerase and nucleic acid h ... | 1991 | 1840612 |
| expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection. | transgenic potato plants, cultivar désirée, were produced that contained the coat protein gene of potato leafroll luteovirus (plrv). the transformed potato plants expressed the plrv coat protein (cp) rna sequences but accumulation of coat protein in transgenic tissues could not be detected. upon inoculation with plrv, the plrv cp rna expressing potato plants showed a reduced rate of virus multiplication. | 1991 | 1883996 |
| the phylogeny of rna-dependent rna polymerases of positive-strand rna viruses. | representative amino acid sequences of the rna-dependent rna polymerases of all groups of positive-strand rna viruses were aligned hierarchiacally, starting with the most closely related ones. this resulted in delineation of three large supergroups. within each of the supergroups, the sequences of segments of approximately 300 amino acid residues originating from the central and/or c-terminal portions of the polymerases could be aligned with statistically significant scores. specific consensus p ... | 1991 | 1895057 |
| the location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mrna. | northern blot analysis of nucleic acid from potato plant tissues and tobacco protoplasts infected with a scottish isolate of potato leafroll luteovirus (plrv) detected the 6 kb genomic rna and one subgenomic rna species of about 2.7 kb; rna extracted from virus particles contained only the genomic species. blotting with small defined probes suggested that the location of the 5' end of the subgenomic rna was between 2380 and 2510 nucleotides from the 3' end of the plrv genome (between 3370 and 35 ... | 1991 | 1940859 |
| development and application of a nonradioactive nucleic acid hybridization system for simultaneous detection of four potato pathogens. | cdna clones of potato virus x (pvxcp strain), potato virus y (pvyo strain), potato leaf roll virus (plrv) and potato spindle tuber viroid (pstv) were used separately or combined for the detection of the corresponding rnas in extracts of infected plants. a general method for the rapid preparation of rna extracts without use of organic solvents (i.e. phenol) was developed for this purpose. plant extracts from a range of field, artificially inoculated germplasm genotypes, micro-propagated and proto ... | 1991 | 2016393 |
| the establishment of rat hybridoma cell lines secreting mcab against strains of potato virus y and analysis of its stability. | the rat splenocytes immunized with potato virus y (pvyn) and ratmyeloma (ir983) were fused by peg (m. w.1450). three kinds of stable hybridoma cell lines secreting specific monoclonal antibodies (mcabs) were derived. one kind of the cell lines producing mcabs reacts to pvyn specifically. another reacts to pvyo specifically. the third one reacts to both of the two strains. tested by the methods of sandwich-elisa and indirect-elisa, all kinds of mcabs did not react to seven plant viruses: tobacco ... | 1990 | 2104212 |
| expression of the genome of potato leafroll virus: readthrough of the coat protein termination codon in vivo. | an antiserum was raised against a fusion protein containing part of the 56k polypeptide (p5) encoded by the open reading frame (orf) at the 3' end of the genome of potato leafroll virus (plrv). this antiserum reacted specifically with 80k and 90k polypeptides in plrv-infected protoplasts, with a 90k polypeptide in infected potato tissue and with a 53k polypeptide in protein extracted from purified particles of plrv. monoclonal antibodies raised against purified plrv particles also reacted with t ... | 1990 | 2230732 |
| ribozymes that cleave potato leafroll virus rna within the coat protein and polymerase genes. | two ribozymes were synthesized which were designed to cleave potato leafroll virus (plrv) positive strand rna within the regions known to encode the viral coat protein and the predicted rna polymerase gene. dna sequences encoding the ribozymes were inserted into the escherichia coli plasmids ptz18r and ptz19r under the control of the bacteriophage t7 promoter and enzymically active rna molecules generated by transcription by t7 rna polymerase in vitro. each ribozyme cleaved its cognate site in r ... | 1990 | 2230733 |
| nucleotide sequences of an australian and a canadian isolate of potato leafroll luteovirus and their relationships with two european isolates. | the genomes of an australian and a canadian isolate of potato leafroll virus have been cloned and sequenced. the sequences of both isolates are similar (about 93%), but the canadian isolate (plrv-c) is more closely related (about 98% identity) to a scottish (plrv-s) and a dutch isolate (plrv-n) than to the australian isolate (plrv-a). the 5'-terminal 18 nucleotide residues of plrv-c, plrv-a, plrv-n and beet western yellows virus have 17 residues in common. in contrast, plrv-s shows no obvious si ... | 1990 | 2313269 |
| relationships among luteoviruses based on nucleic acid hybridization and serological studies. | the luteoviruses barley yellow dwarf virus (bydv-pav and bydv-rpv), bean leaf roll virus (blrv) beet western yellows virus (bwyv), carrot red leaf virus, potato leaf roll virus, and soybean dwarf virus (sdv) were compared by hybridization with random cdna probes and serologically with polyclonal antisera. for hybridizations, filters had each rna blotted in duplicate dots at 10 ng/dot. random-primed cdna probes were prepared from 300 ng of each rna and used to probe filters at three levels of str ... | 1990 | 2338398 |
| nucleotide sequence and organization of potato leafroll virus genomic rna. | the nucleotide sequence of the genomic rna of potato leafroll virus was determined and its genetic organization deduced. the rna is 5882 nucleotides long and contains 6 open reading frames (orfs) encoding proteins of 70, 70, 56, 28, 23 and 17 kda. the putative genes for the coat protein (23 kda) and the rna-dependent rna polymerase (70 kda) were identified by interviral amino acid sequence homologies. for expression of the different orfs, translational frameshift and readthrough events are propo ... | 1989 | 2466700 |
| the use of peroxidase anti-peroxidase (pap) complexes in the detection of plant viruses by elisa. | an elisa test system for detection of plant viruses in field samples is described, based on the unlabelled antibody method using a peroxidase-antiperoxidase (pap) complex. novel features of the system include the use of acid-treated naked bacteria as combined carrier-adjuvants for the production of rabbit antiviral antibodies, and the use of acid-treated chicken antibodies (igy) for antigen trapping in the elisa. systems for detection of potato virus y (pvy), potato leafroll virus (plrv), grapev ... | 1989 | 2685004 |
| identification and characterization of the potato leafroll virus putative coat protein gene. | complementary dna clones representing approximately 6100 nucleotides of potato leafroll virus (plrv) were generated, restriction-mapped, and partially sequenced. within one of the cdna clones an open reading frame (orf) encoding a 23k protein was identified and further characterized. amino acid sequence comparison of this protein showed significant homology (47.1%) with the barley yellow dwarf virus (bydv-pav) coat protein. this and other observations suggested that this gene encodes the plrv co ... | 1989 | 2732704 |
| nucleotide sequence of potato leafroll luteovirus rna. | a sequence of 5987 nucleotides is reported for the rna of potato leafroll luteovirus (plrv). the sequence contains six large open reading frames, and non-coding regions of 174 nucleotides at the 5' end, 141 nucleotides at the 3' end and 197 nucleotides between two large blocks of coding sequences. the 5' coding region encodes two polypeptides of 28,000 (28k) and 70k which overlap in different reading frames and circumstantial evidence suggests that the third open reading frame in the 5' block is ... | 1989 | 2732710 |
| cloning of the gene for the capsid protein of potato leafroll virus. | dna complementary to the rna of purified potato leafroll virus (plrv) was synthesized and cloned into the lambda insertion vector nm1149. overlapping plrv-specific cdna clones were isolated that represent some 80% of the viral genome. sequences coding for the capsid protein were identified by subcloning size-selected cdnas into the lambda expression vector gt11 and screening with plrv-specific antisera. the gene for the viral capsid protein was shown to reside in the 3' terminal half of the geno ... | 1989 | 2751428 |
| nucleotide sequence of the potato leafroll virus coat protein gene. | | 1989 | 2922295 |
| immunological properties and biological function of monoclonal antibodies to tobacco mosaic virus. | monoclonal antibodies to tmv vulgare produced in hybridoma cultures as well as in ascitic fluid were characterized according to their reactivity with the virion and/or the coat protein monomer thus revealing specificity for epitopes, cryptotopes or neotopes. different patterns of crossreactivity of these monoclonal antibodies with tmv strains dahlemense and holmes' ribgrass occurred. some monoclonal antibodies showed stronger reactivity with these strains than with the immunizing strain. the mon ... | 1986 | 3942505 |
| structural analysis of p19 and p24 core polypeptides of primate lymphotropic retroviruses (plrv). | core polypeptides of primate lymphotropic retroviruses (plrv) have very similar molecular weights. to discriminate between individual plrvs we have compared two-dimensional tryptic peptide maps of 125i-labeled core polypeptides p19 and p24 of 11 isolates originating from humans and six simian species. peptide maps showed homologies between all the simian viruses and the human isolates, but they were completely different from those of human t-cell leukemia virus type iii (htlv-iii). in general p2 ... | 1986 | 3952986 |
| purification and electron microscopy of potato leafroll virus. | | 1969 | 4186521 |
| dna-like properties of the nucleic acid of potato leafroll virus. | | 1973 | 4768620 |
| transmission of potato leafroll virus by aphids after feeding on virus preparations from aphids and plants. | | 1968 | 5677801 |
| isolation and electron microscopy of potato leafroll virus from plants. | | 1968 | 5677802 |
| the purification of potato leafroll virus from its vector myzus persicae. | | 1967 | 6017406 |
| structural and epidemiological features of primate lymphotropic retroviruses. | primate lymphotropic retroviruses (plrv) have been isolated from man and various species of old world monkeys. the human isolates adult t-cell leukemia virus (atlv) or human t-cell leukemia virus (htlv-i) are endemic among japanese in the south-west of the country as well as africans in both africa and america. in these populations atl and its lymphoma variant are also endemic and all these patients harbor the virus and have serum antibodies to viral env gene polypeptides gp68, gp46, p21 as well ... | 1984 | 6100632 |
| isopycnic centrifugation of plant viruses in nycodenz density gradients. | isopycnic centrifugation of plant viruses in density gradients prepared from a new non-ionic medium (nycodenz) was investigated. particle density of luteo-, tymo-, nepo-, cocksfoot mild mosaic, tobamo-, hordei-like, potex- and potyviruses in buffered solutions of nycodenz ranged between 1.23 and 1.28 g/ml and did not strictly reflect their nucleic acid and protein composition. isopycnic centrifugation of several partially purified viruses yielded preparations which were pure by electron microsco ... | 1984 | 6520198 |
| electro-blot radioimmunoassay of virus-infected plant sap - a powerful new technique for detecting plant viruses. | a new technique for detecting viruses in plant sap is described. it consists of sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the infected plant sap, electrophoretic transfer of protein bands to activated paper by the electro-blot technique, the subsequent probing of the viral coat protein band by specific antiserum (prepared against intact virus), and detection of immune complex with 125 i-labelled protein a. the technique successfully detected tobacco mosaic virus at a sap dilu ... | 1982 | 7061671 |
| changes in the amino acid sequence of the coat protein readthrough domain of potato leafroll luteovirus affect the formation of an epitope and aphid transmission. | potato leafroll luteovirus (plrv) is transmitted naturally by aphids, but two isolates (15 and v) are known to be only poorly transmissible (pat); these isolates are also distinct in that their particles lack an epitope present in transmissible (hat) isolates of plrv (tamada et al., ann. appl. biol. 104, 107-116, 1984; massalski and harrison, j. gen. virol. 68, 1813-1821, 1987). virus cultures propagated vegetatively for several years in potato plants from the source of isolate v were shown stil ... | 1994 | 7513925 |
| differential response to frameshift signals in eukaryotic and prokaryotic translational systems. | the genomic rna of beet western yellows virus (bwyv) contains a potential translational frameshift signal in the overlap region of open reading frames orf2 and orf3. the signal, composed of a heptanucleotide slippery sequence and a downstream pseudoknot, is similar in appearance to those identified in retroviral rnas. we have examined whether the proposed bwyv signal functions in frameshifting in three translational systems, i.c. in vitro in a reticulocyte lysate or a wheat germ extract and in v ... | 1993 | 7680118 |
| nucleotide sequence of rna of a polish isolate of potato leafroll luteovirus. | the nucleotide sequence of the genomic rna of a polish isolate of a potato leafroll virus was determined. some variations between the determined sequences were observed. a comparison of the frequency of sequence variants in particular regions of the genome is presented. | 1994 | 7732757 |
| digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (pstvd), potato virus y (pvy), and potato leafroll virus (plrv). | a molecular probe, p3pot, was constructed of pstvd, pvy, plrv cdna fragments introduced into puc18 vector. sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3pot probe. this makes p3pot probe an useful tool for the routine testing, and selection of virus-free potatoes. | 1994 | 7732766 |
| molecular cloning and nucleotide sequence of the coat protein gene of a cuban isolate of potato leafroll virus and its expression in escherichia coli. | total rna from infected physalis floridana was isolated to generate complementary dna corresponding to the coat protein (gp) gene of a cuban isolate of potato leaf roll virus (plrv). this cdna was amplified by the polymerase chain reaction (pcr) and cloned into the bacterial expression vectors pex(1-3) for fusion protein expression in e. coli. the product was detected by antibodies specific for the plrv cp. the coding sequence of the cp gene was determined, and the predicted length of the cp was ... | 1994 | 7871764 |
| coat protein sequences of rmv-like strains of barley yellow dwarf virus separate them from other luteoviruses. | illinois (il) and minnesota (mn) rmv-like strains of barley yellow dwarf virus (bydv) were identified from maize displaying red leaf symptoms by enzyme-linked immunosorbent assay (elisa) using antiserum against a new york strain (bydv-rmv-ny). some il and mn strains, but not the ny strain, could be detected by elisa with a monoclonal antibody raised against bydv-rpv-ny. the region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequen ... | 1994 | 7928284 |
| endosymbiotic bacteria associated with circulative transmission of potato leafroll virus by myzus persicae. | in order to understand the molecular mechanisms underlying circulative transmission of potato leafroll virus (plrv) by aphids, we screened myzus persicae proteins as putative plrv binding molecules using a virus overlay assay of protein blots. in this way, we found that purified plrv particles exhibited affinity for five aphid proteins. the one most readily detected has an m(r) of 63k, and was identified as symbionin. this is the predominant protein synthesized by the bacterial endosymbiont of t ... | 1994 | 7931143 |
| nucleotide sequence of cucurbit aphid-borne yellows luteovirus. | the nucleotide sequence (5669 residues) of the genomic rna of cucurbit aphid-borne yellows luteovirus (cabyv) is presented. analysis of genome organization and sequence homologies indicate that cabyv is a member of luteovirus subgroup 2 (other sequenced members: beet western yellows virus, potato leafroll virus, and barley yellow dwarf virus, rpv isolate) and appears to be most closely related to beet western yellows virus. | 1994 | 8030201 |
| enhancement of resistance to potato leafroll virus multiplication in potato by combining the effects of host genes and transgenes. | four potato clones with host gene-mediated resistance to potato leafroll virus (plrv) multiplication were transformed with the plrv coat protein (cp) gene. plants of lines expressing high levels of transcript were highly resistant to plrv multiplication; virus concentration was only 20-40 ng/g of leaf, which is approximately 1% of the concentration reached in susceptible cultivars. the effects of the transgenic and host-derived resistance genes appear to be additive. | 1994 | 8075424 |
| a method combining immunocapture and pcr amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. | a method for the detection of rna viral and subviral plant pathogens was developed that combines pathogen partial purification by solid-phase adsorbed antibodies, reverse transcriptional-polymerase chain reaction (rt-pcr) and quantitation of the amplified products by fluorescence. the reverse transcription of the rna is performed directly on the retained material without any previous thermal or chemical disruption of the virus particles. the whole procedure can be carried out in a microtiter pla ... | 1993 | 8113346 |
| soybean dwarf luteovirus contains the third variant genome type in the luteovirus group. | complementary dnas covering the entire rna genome of soybean dwarf luteovirus (sdv) were cloned and sequenced. computer analysis of the 5861 nucleotide sequence revealed five major open reading frames (orfs) possessing conservation of sequence and organisation with known luteovirus sequences. comparative analyses of the genome structure show that sdv shares sequence homology and features of gene organisation with barley yellow dwarf virus (pav isolate) in the 5' half of the genome, yet is more c ... | 1994 | 8291248 |
| the intestine is a site of passage for potato leafroll virus from the gut lumen into the haemocoel in the aphid vector, myzus persicae sulz. | four detection techniques, three of which gave reliable identification of the virus particles, were used to locate potato leafroll virus (plrv) in the alimentary canal of its main aphid vector, myzus persicae sulz: immunofluorescence on cryostat sections, conventional transmission electron microscopy on ultrathin sections and immune electron microscopy with gold labeling, either prior to or after fixation-embedding. each method clearly showed the presence of the virus in the intestine epithelium ... | 1993 | 8347080 |
| purification of potato leaf roll virus. | | 1993 | 8367402 |
| structural requirements for efficient translational frameshifting in the synthesis of the putative viral rna-dependent rna polymerase of potato leafroll virus. | the putative rna-dependent rna polymerase of potato leafroll luteovirus (plrv) is expressed by -1 ribosomal frameshifting in the region where the open reading frames (orf) of proteins 2a and 2b overlap. the signal responsible for efficient frameshift is composed of the slippery site uuuaaau followed by a sequence that has the potential to adopt two alternative folding patterns, either a structure involving a pseudoknot, or a simple stem-loop structure. to investigate the structure requirements f ... | 1993 | 8502558 |
| sequence comparison and classification of beet luteovirus isolates. | three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (bwyv) and beet mild yellowing virus (bmyv) from europe, iran and the usa. the first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked to this host range specificity are 2 single base pair changes in a 1,200 nucleotide region of the genome. it is proposed that the european bwyv strains that can be transferred at l ... | 1995 | 8572940 |
| detection of potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potential epidemiological application. | a reverse transcription and polymerase chain reaction (rt-pcr) system was developed using two 20-mer primers located in the potato leafroll virus (plrv) capsid gene. a 336-bp pcr product was detected from aphids (myzus persicae) which had been fed on plrv-infected plants. the pcr band was specific to plrv as determined by southern blots and detection by a plrv-specific probe. as little as 5 min exposure of aphids to plrv-infected leaves resulted in the presence of plrv-specific bands in 13% of a ... | 1995 | 8576303 |
| non-canonical translation mechanisms in plants: efficient in vitro and in planta initiation at auu codons of the tobacco mosaic virus enhancer sequence. | the 5' untranslated leader (omega sequence) of tobacco mosaic virus (tmv) genomic rna was utilized as a translational enhancer sequence in expression of the 17 kda putative movement protein (pr17) of potato leaf roll luteovirus (plrv). in vitro translation of rnas transcribed from appropriate chimeric constructs, as well as their expression in transgenic potato plants, resulted in the expected wild-type pr17 protein, as well as in larger translational products recognized by pr17-specific antiser ... | 1996 | 8628648 |
| assembly of virus-like particles in insect cells infected with a baculovirus containing a modified coat protein gene of potato leafroll luteovirus. | dna encoding the coat protein (p3) of a scottish isolate of potato leafroll virus (plrv) was inserted into the genome of autographa californica nucleopolyhedrovirus (acnpv) such that the coat protein was expressed either in an unmodified form or with the addition of the amino acid sequence mhhhhhhgddddkdamg at the n terminus (p3-6h). insect cells infected with these recombinant baculoviruses accumulated substantial amounts of p3 and p3-6h. p3 could not be recovered from cell extracts unless it w ... | 1996 | 8757974 |
| ultrastructural study of acquisition and retention of potato leafroll luteovirus in the alimentary canal of its aphid vector, myzus persicae sulz. | the chronology of plrv acquisition and retention by myzus persicae was investigated using electron microscopy. examination demonstrated a rapid translocation of the virus through the intestine into the haemocoel. indeed, viral particles could be observed in the intestinal epithelial cells, then in the haemocoel, 4 and 8 h, respectively, after their arrival in the lumen of the alimentary canal. however, the virus accumulated in the intestinal epithelial cells. in these cells, the first viral part ... | 1996 | 8774687 |
| detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. | a reverse transcription polymerase chain reaction (rt-pcr) assay was designed to amplify stylet-borne potato virus yo (pvyo) in aphids using primers located in the viral capsid gene. a 480 bp long product was detected in aphids exposed to pvyo-infected potato plants. approximately 40% of myzus persicae and 15% of aphis nasturtii exposed briefly to pvyo-infected plants acquired the virus. this rate of acquisition by both species of aphids was typical of our earlier observation of the virus transm ... | 1996 | 8793847 |
| the potato leafroll virus 17k movement protein is phosphorylated by a membrane-associated protein kinase from potato with biochemical features of protein kinase c. | the 17 kda protein (pr17), the phloem-limited movement protein (mp) of potato leafroll luteovirus (plrv), is associated with membranous structures and localized to plasmodesmata [tacke et al. (1993) virology 197, 274-282; schmitz, j. (1995) ph.d. thesis, university of cologne]. in planta the protein is predominantly present in its phosphorylated form, but it is rapidly dephosphorylated during isolation under native conditions. in an effort to examine the nature of the protein kinase(s) involved ... | 1997 | 9001398 |
| short and long distance spread of potato leafroll luteovirus: effects of host genes and transgenes conferring resistance to virus accumulation in potato. | potato leafroll luteovirus (plrv) movement through phloem of plrv-resistant potato clones was examined in experiments in which stem pieces were grafted either between infected rootstocks and virus-free susceptible scions or between infected scions and virus-free susceptible rootstocks. these test plants permitted either upwards or downwards virus movement into the susceptible tissue. resistant potato clones had either host gene-mediated resistance (h-mr) or transgene-mediated resistance (t-nr, c ... | 1997 | 9010310 |
| a scfv-alkaline phosphatase fusion protein which detects potato leafroll luteovirus in plant extracts by elisa. | a single chain fv antibody fragment (scfv) was obtained from a synthetic phage-antibody library after four rounds of selection against purified preparations of potato leafroll luteovirus (plrv). nucleotide sequence analysis showed that the scfv belongs to the human v(h)3 family. dna encoding the scfv was sub-cloned into pdap2 such that a scfv-alkaline phosphatase fusion protein was produced by transformed bacteria following induction by isopropyl-beta-d-thiogalactopyranoside (iptg). the fusion p ... | 1997 | 9015295 |
| expression of the potato leafroll virus orf0 induces viral-disease-like symptoms in transgenic potato plants. | the role of the open reading frame 0 (orf0) of luteoviruses in the viral infection cycle has not been resolved, although the translation product (p28) of this orf has been suggested to play a role in host recognition. to investigate the function of the potato leafroll luteovirus (plrv) p28 protein, transgenic potato plants were produced containing the orf0. in the lines in which the orf0 transcripts could be detected by northern (rna) analysis, the plants displayed an altered phenotype resemblin ... | 1997 | 9057321 |
| in vivo expression of a full-length cdna copy of potato leafroll virus (plrv) in protoplasts and transgenic plants. | a full-length cdna copy (plrvfl) of potato leafroll virus (plrv) was constructed and examined in vivo for its biological activities by transient expression experiments with plasmid dna or in vitro transcribed rna. in addition, plrvfl cdna was stably introduced into the genome of potato plants by agrobacterium-mediated leaf disc transformation. both transient and stable expression of plrvfl resulted in the synthesis of genomic and subgenomic plrv rnas. transgenic plants accumulated the 17-kda mov ... | 1997 | 9065694 |
| expression patterns of vascular-specific promoters rolc and sh in transgenic potatoes and their use in engineering plrv-resistant plants. | the expression patterns of gus fusion constructs driven by the agrobacterium rhizogenes rolc and the maize sh (shrunken; sucrose synthase-1) promoters were examined in transgenic potatoes (cv. atlantic). rolc drove high-level gene expression in phloem tissue, bundle sheath cells and vascular parenchyma, but not in xylem or non-vascular tissues. sh expression was exclusively confined to phloem tissue. potato leafroll luteovirus (plrv) replicates only in phloem tissues, and we show that when rolc ... | 1997 | 9132064 |
| evidence for heterologous encapsidation of potato spindle tuber viroid in particles of potato leafroll virus. | the aphid myzus persicae (sulz.) was shown to transmit potato spindle tuber viroid (pstvd) to potato clone dto-33 from source plants doubly infected with potato leafroll virus (plrv) and pstvd. transmission was of the persistent type and did not occur when the insects were allowed to feed on singly infected plants. only low levels of pstvd were associated with purified plrv virions, but its resistance to digestion with micrococcal nuclease indicates that the viroid rna is encapsidated within the ... | 1997 | 9191909 |
| direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method nasba. | nasba, an isothermal amplification method for nucleic acids, was applied to the detection of rna of potato leafroll virus (plrv) in a single enzymatic reaction at 41 degrees c. a set of primers was selected from the coat protein open reading frame sequence of plrv to allow amplification of viral rna. the nasba reaction products were visualized after electrophoresis by ethidium bromide or acridine orange staining. the specificity of the amplification products was validated by northern blot analys ... | 1997 | 9220387 |
| studies on the translation mechanism of subgenomic rna of potato leafroll virus. | the expression of open reading frames located on the subgenomic rna (sgrna) has been studied in an in vitro transcription and translation system. the obtained results indicate: a) translation of sgrna occurs according to the scanning model since the insertion of palindrome (delta g0 = -61 kcal/mol) prevents the initiation of translation; b) orf6 is translated by suppression of the stop codon separating orf4 from orf6 and the presence of suppressor trna is necessary for the readthrough; c) the pr ... | 1997 | 9241356 |
| the genome-linked protein of potato leafroll virus is located downstream of the putative protease domain of the orf1 product. | the sequence of the 32 n-terminal amino acids of the protein (vpg) which is covalently linked to the rna of potato leafroll virus has been determined. the obtained vpg sequence mapped to position 400 to 431 of the plrv orf1 product, downstream of the putative protease domain and in front of the rna-dependent rna polymerase. comparison with other viral sequences revealed significant similarities with the orf1 products of beet western yellows virus, cucurbit aphid-borne yellows virus, and beet mil ... | 1997 | 9268161 |
| in situ localization of the putative movement protein (pr17) from potato leafroll luteovirus (plrv) in infected and transgenic potato plants. | the potato leafroll virus (plrv) 17-kda protein (pr17), the putative movement protein for this phloem-limited luteovirus, was localized on ultrathin sections of leaves from plrv-infected and transgenic potato plants. the transgenic plants expressed the entire viral genome from a full-length cdna copy (plrvfl) or only the gene encoding pr17 (orf4) under the control of the cauliflower mosaic virus 35s promoter. virus-infected and plrvfl-transgenic plants developed symptoms typical of virus infecti ... | 1997 | 9281511 |
| expression of a luteoviral movement protein in transgenic plants leads to carbohydrate accumulation and reduced photosynthetic capacity in source leaves. | elucidating the role of viral genes in transgenic plants revealed that the movement protein (mp) from tobacco mosaic virus is responsible for altered carbohydrate allocation in tobacco and potato plants. to study whether this is a general feature of viral mps, the movement protein mp17 of potato leafroll virus (plrv), a phloem-restricted luteovirus, was constitutively expressed in tobacco plants. transgenic lines were strongly reduced in height and developed bleached and sometimes even necrotic ... | 1997 | 9418046 |
| potato leafroll virus binds to the equatorial domain of the aphid endosymbiotic groel homolog. | a groel homolog with a molecular mass of 60 kda, produced by the primary endosymbiotic bacterium (a buchnera sp.) of myzus persicae and released into the hemolymph, has previously been shown to be a key protein in the transmission of potato leafroll virus (plrv). like other luteoviruses and pea enation mosaic virus, plrv readily binds to extracellular buchnera groel, and in vivo interference in this interaction coincides with reduced capsid integrity and loss of infectivity. to gain more knowled ... | 1998 | 9420234 |
| in planta transcription of a second subgenomic rna increases the complexity of the subgroup 2 luteovirus genome. | the genetic information of potato leafroll virus (plrv), a typical member of the subgroup 2 luteoviruses, is contained in a single-stranded (+) sense rna of approximately 5.9 kb. a single subgenomic rna (sgrna1) of approximately 2.3 kb has been characterized as the mrna for the 3' clustered viral open reading frames orf3, orf3/5 and orf4. here we demonstrate by northern blot analyses of polysomal rnas from plrv-infected solanum tuberosum and physalis floridana plants that, as with luteoviruses b ... | 1998 | 9421494 |
| expression of a cdna encoding phytolacca insularis antiviral protein confers virus resistance on transgenic potato plants. | to develop an antiviral agent and virus-resistant plants, a cdna clone encoding phytolacca insularis antiviral protein (pip) was isolated from a cdna library constructed with poly(a)+ rna purified from leaves of p. insularis. the pip cdna contains an open reading frame encoding 307 amino acids. the deduced amino acid sequence includes a putative signal sequence of 22 amino acids at the n-terminus. the amino acid sequence of pip shares 84% homology with that of the pokeweed antiviral protein (pap ... | 1997 | 9509425 |
| molecular beacon probes combined with amplification by nasba enable homogeneous, real-time detection of rna. | molecular beacon probes can be employed in a nasba amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. a molecular beacon, designed to hybridize within the target sequence, was introduced into nasba reactions that amplify the genomic rna of potato leafroll virus (plrv). during amplification, the probe anneals to the antisense rna amplicon generated by nasba, producing a specific fluorescent signal that can be monitored in real-time. the assay is ... | 1998 | 9547273 |
| genetic engineering of potato for broad-spectrum protection against virus infection. | transgenic potato plants expressing mutant alleles of plrv orf4, the gene for the movement protein pr17 of this luteovirus, were generated for broad-range protection against virus infection. when tested for protection against infection by plrv, all transgenic lines showed a significant reduction of virus antigen. potato lines accumulating n- or c-terminally extended plrv pr17 mutant proteins were resistant to infection by the unrelated potato viruses pvy and pvx. transgenic lines that did not ex ... | 1996 | 9634829 |
| infectious transcripts from cloned cdna of potato leafroll luteovirus. | infectious transcripts play a key role in the research on plant viruses at the molecular level. a number of cdna clones covering the whole genome of the polish isolate of potato leafroll virus were constructed. four overlapping clones were selected and assembled using restriction sites. the full copy was positioned between t7 rna polymerase promoter and unique scai site. the full-length capped transcripts of the sequence of the viral genome synthesised in vitro were able to replicate in protopla ... | 1998 | 9821890 |
| immunological analysis of potato leafroll luteovirus (plrv) p1 expression identifies a 25 kda rna-binding protein derived via p1 processing. | mono- and polyclonal antibodies directed against different domains of the potato leafroll luteovirus (plrv) p1 (orf1) protein were applied to the analysis of p1 expression during plrv replication in planta. western analyses detected p1 and a protein of approximately 25 kda (p1-c25) that accumulated to readily detectable amounts in plrv-infected plants, but was not detected by in vitro cell-free translation of p1. p1-c25 represents the c-terminus of p1 and is a proteolytic cleavage product produc ... | 1999 | 9862960 |
| properties of a panel of single chain variable fragments against potato leafroll virus obtained from two phage display libraries. | twelve single chain variable fragment (scfv) antibodies that bind to particles of potato leafroll virus (plrv) were obtained from two naive phage display libraries. phages were selected against plrv particles or dissociated plrv particles immobilised onto tubes. individual plrv-binding scfv were identified by elisa, after their expression either fused to the surface of phage particles, or as soluble scfv (scfv-c-myc), or as scfv-alkaline phosphatase fusion proteins (scfv-ap), obtained by subclon ... | 1999 | 10488774 |
| transformation of tobacco and potato with cdna encoding the full-length genome of potato leafroll virus: evidence for a novel virus distribution and host effects on virus multiplication. | a full-length cdna copy of the genome of potato leafroll virus (plrv) was introduced into the genome of tobacco and potato plants by agrobacterium tumefaciens-mediated transformation. transgenic lines were obtained in which the transgene was readily detected by pcr with dna extracted from t(1) tobacco seedlings and clonally multiplied potato plants. plrv-specific genomic and sub- genomic rnas, coat protein antigen and virus particles were detected in transgenic plants. aphids fed on the transgen ... | 1999 | 10580042 |
| a solvent-free, rapid and simple virus rna-release method for potato leafroll virus detection in aphids and plants by reverse transcription polymerase chain reaction. | a one-step, rapid and economical method for potato leafroll virus (plrv) rna release that is applicable to the use on a microcentrifuge scale is described. discs (3-6 mm diameter) from leaves, petioles, stems, and tubers of potato plants were incubated in microcentrifuge tubes with detergent solution. the supernatants were used directly for reverse transcription (rt) and polymerase chain reaction (pcr). of the seven nonionic detergents of the tritonx series evaluated, triton x405r was the most e ... | 1999 | 10598080 |
| tagging potato leafroll virus with the jellyfish green fluorescent protein gene. | a full-length cdna corresponding to the rna genome of potato leafroll virus (plrv) was modified by inserting cdna that encoded the jellyfish green fluorescent protein (gfp) into the p5 gene near its 3' end. nicotiana benthamiana protoplasts electroporated with plasmid dna containing this cdna behind the 35s rna promoter of cauliflower mosaic virus became infected with the recombinant virus (plrv-gfp). up to 5% of transfected protoplasts showed gfp-specific fluorescence. progeny virus particles w ... | 2000 | 10675399 |
| identifying the determinants in the equatorial domain of buchnera groel implicated in binding potato leafroll virus. | luteoviruses avoid degradation in the hemolymph of their aphid vector by interacting with a groel homolog from the aphid's primary endosymbiotic bacterium (buchnera sp.). mutational analysis of groel from the primary endosymbiont of myzus persicae (mpb groel) revealed that the amino acids mediating binding of potato leafroll virus (plrv; luteoviridae) are located within residues 9 to 19 and 427 to 457 of the n-terminal and c-terminal regions, respectively, of the discontinuous equatorial domain. ... | 2000 | 10775590 |
| duplex rt-pcr: reagent concentrations at reverse transcription stage affect the pcr performance. | test conditions for the simultaneous detection of potato leafroll virus (plrv) and potato virus y (pvy) in dormant tubers and leaves by reverse transcription-polymerase chain reaction (rt-pcr) were optimized. various factors optimized at the reverse transcription (rt) stage rather than at the amplification (pcr) stage affected the outcome. in the simplex rt-pcr a onefold dntps concentration (0.5 mm) was sufficient in yielding a plrv or pvy band. in contrast, the duplex rt-pcr required a minimum ... | 2000 | 10785287 |
| potato leafroll virus protein p1 contains a serine proteinase domain. | the multi-domain potato leafroll virus replicase protein p1 was expressed in insect cells from the polyhedrin promoter of autographa californica nucleopolyhedrovirus. using antisera raised against p1, it was shown that p1 was cleaved near the vpg in insect cells in a manner similar to that in plant cells, to produce a approximately 27 kda c-terminal fragment. furthermore, it was shown that the proposed serine proteinase-like domain within p1 is responsible for this processing and that this can o ... | 2000 | 10859393 |
| mutational study reveals that tertiary interactions are conserved in ribosomal frameshifting pseudoknots of two luteoviruses. | expression of the putative replicase of potato leafroll virus (plrv) is regulated by -1 ribosomal frameshifting in which a primary viral transcript has two overlapping open reading frames (orfs). a region of 39 nt at the junction of the two orfs is essential for frameshifting to occur. it has been shown to harbor two signals, one active on the level of the primary structure, termed the slippery sequence, and one component that forms a secondary or tertiary level structure, described as either a ... | 2000 | 10943894 |
| mechanical transmission of potato leafroll virus. | like typical luteoviruses, potato leafroll virus (plrv) cannot be transmitted mechanically by rubbing plants with solutions containing virus particles. however, plrv was found to be mechanically transmissible from extracts of plants that had been inoculated by viruliferous aphids and then post-inoculated with pea enation mosaic virus-2 (pemv-2). unlike the asymptomatic infections induced by either virus alone, double infections in nicotiana benthamiana induced necrotic symptoms with some line pa ... | 2000 | 11038393 |
| effect of genomic and subgenomic leader sequences of potato leafroll virus on gene expression. | the effect of the genomic and subgenomic leader sequence of potato leafroll polerovirus on the efficiency of translation of the downstream located genes has been studied. the results obtained in vitro and in vivo indicate that neither leader sequence functions as translational enhancer, a generally important feature of leader sequences. deletion analyses demonstrated that both leader sequences not only decrease translation of the downstream located genes but also alter the ratio of the synthesiz ... | 2000 | 11056217 |
| the passage of potato leafroll virus through myzus persicae gut membrane regulates transmission efficiency. | potato leafroll virus (plrv) is transmitted by aphids in a persistent manner. although virus circulation within the aphid leading to transmission has been well characterized, the mechanisms involved in virus recognition at aphid membranes are still poorly understood. one isolate in our collection (plrv-14.2) has been shown to be non- or only poorly transmitted by some clones of aphids belonging to the myzus persicae complex. to determine where the transmission process was blocked within the aphi ... | 2001 | 11125153 |
| some epidemiological approaches to the control of aphid-borne virus diseases in seed potato crops in northern europe. | an account is given of progress during the last 30 years in knowledge of the epidemiology of diseases caused by aphid-borne viruses in seed potato production areas of northern europe. during this period, potato leafroll virus (plrv) and strains of potato virus y (pvy(o), pvy(n)) were consistently the most prevalent and harmful. the main factors influencing spread involve the amount of initial virus inoculum in seed crops, agricultural practices in relation to seed potato production, the status o ... | 2000 | 11137160 |
| extreme resistance to potato leafroll virus in potato cv. russet burbank mediated by the viral replicase gene. | high levels of field resistance to potato leafroll virus (plrv; genus: polerovirus; family: luteoviridae) were achieved by expression of the unmodified, full-length plrv replicase gene in potato plants cv. russet burbank. a high degree of resistance was also achieved, but less frequently, by expression of a truncated construct of the replicase gene. in limited testing, neither miss-frame nor antisense constructs of the replicase gene conferred resistance. the degree of resistance expressed among ... | 2000 | 11137161 |
| a novel usage of random primers for multiplex rt-pcr detection of virus and viroid in aphids, leaves, and tubers. | a multiplex reverse transcription polymerase chain reaction (m-rt-pcr) was developed for the simultaneous detection of five potato viruses and a viroid. the synthesis of cdnas used for amplification was primed by hexanucleotides (random primers, rp). an rna extraction procedure employing dnase i, is routinely used to isolate potato viruses and viroid (potato virus s, pvs; potato leafroll virus, plrv; potato virus x, pvx; potato virus a and y, pva, pvy; and potato spindle tuber viroid, pstvd) fro ... | 2001 | 11164484 |
| mechanical transmission of poleroviruses. | previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. biolistic inoculation allowed for the first time the mechanical transmission of beet western yellows virus (bwyv) and potato leafroll virus (plrv) to several host plants. inoculation with purified preparations and viral rna extracts of plrv resulted in 30-50% systemically infected nicotiana occidentalis p1 plants and 15-30% infected nicotiana clevelandii plants. particle bombardment was also used succe ... | 2001 | 11164501 |
| development of a multiplex amplidet rna for the simultaneous detection of potato leafroll virus and potato virus y in potato tubers. | a novel isothermal multiplex amplidet rna system is described for the simultaneous amplification and detection of potato leafroll virus (plrv) and potato virus y (pvy) in seed potatoes. the risk of contamination by carry-over during diagnostic screening is eliminated by performing the reaction in a single closed tube. the viruses present in a sample are identified using differently coloured molecular beacons directed to a selected virus-specific sequence within the amplicon formed during amplifi ... | 2001 | 11311350 |
| compositional analysis of tubers from insect and virus resistant potato plants. | genetically modified potato plants that are resistant to the colorado potato beetle, plus either the potato leaf roll virus or potato virus y, have recently been commercialized. as part of the safety assessment for plants produced by modern biotechnology, the composition of the food/feed must be compared to that of the food/feed produced by an equivalent plant variety from a conventional source. the composition of important nutritional and antinutritional factors in tubers produced by virus- and ... | 2000 | 11312768 |
| potyviral helper-component proteinase expressed in transgenic plants enhances titers of potato leaf roll virus but does not alleviate its phloem limitation. | coinfection of nicotiana benthamiana with potato virus a (pva, a potyvirus) and potato leaf-roll virus (plrv, a luteovirus) induces a synergistic interaction manifested by enhanced titers of plrv. the helper component proteinase (hc-pro) of potyviruses is involved in viral vascular movement and suppression of an antiviral defense mechanism in plants. data of our study showed that accumulation of plrv in transgenic n. benthamiana expressing the pva hc-pro was enhanced on average by 4.5-fold, as c ... | 2001 | 11336553 |
| mutational analysis of the proteinase function of potato leafroll virus. | cdna expression vectors of potato leafroll virus (plrv) were used to analyse specific mutations in the proteinase and replicase domains of the proteins encoded by orf1 and orf2. agrobacterium-mediated dna transfer was used to introduce a plrv rna expression unit, controlled by the 35s promoter of cauliflower mosaic virus, into potato leaf cells. expression of unmodified plrv cdna led to the replication of viral genomic and subgenomic rnas and accumulation of the viral capsid protein, whereas alt ... | 2001 | 11369899 |
| the orfo product of potato leafroll virus is indispensable for virus accumulation. | using a cdna expression cassette in combination with agroinoculation of potato leaf discs we have investigated the role the protein encoded by orf0 of potato leafroll virus (plrv) and have shown its importance for virus accumulation. two mutations introduced into orf0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells. they did not, however, affect translation of orf1 and orf2. we therefore conclude that orf0 ... | 2001 | 11369900 |
| analysis of a three-dimensional structure of potato leafroll virus coat protein obtained by homology modeling. | viruses of the family luteoviridae are ssrna plant viruses that have particles that exhibit icosahedral symmetry. to identify the residues that might be exposed on the surface of the potato leafroll virus (plrv; genus polerovirus, family luteoviridae) capsid, and therefore involved in biological interactions, we performed a structural analysis of the plrv coat protein (cp) on the basis of comparisons with protein sequences and known crystal structures of cps of other viruses. the cp of plrv disp ... | 2001 | 11448160 |
| umbravirus gene expression helps potato leafroll virus to invade mesophyll tissues and to be transmitted mechanically between plants. | potato leafroll virus (plrv) was mechanically transmissible when inocula also contained the umbravirus pea enation mosaic virus-2 (pemv-2). in plants infected with plrv and pemv-2, plrv accumulated in clusters of mesophyll cells in both inoculated and systemically infected leaves. no transmissions were obtained by coinoculation with potato virus y, potato virus x (pvx), tobacco mosaic virus, or cucumber mosaic virus (cmv), although plrv was transmissible from mixtures with cmv(orf4) (a recombina ... | 2001 | 11485404 |
| transgenic resistance in potato plants expressing potato leaf roll virus (plrv) replicase gene sequences is rna-mediated and suggests the involvement of post-transcriptional gene silencing. | genetically engineered expression of replicase encoding sequences has been proposed as an efficient system to confer protection against virus diseases by eliciting protection mechanisms in the plant. potato leaf-roll was one of the first diseases for which this kind of protection was engineered in potato plants. however, details of the protecting mechanism were not reported, so far. the orf2b of an argentinean strain of plrv was cloned and sequenced finding 94% and 97% of homology with australia ... | 2001 | 11556710 |
| sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection by rt-pcr. | phenolic compounds from plant tissues inhibit reverse transcription-polymerase chain reaction (rt-pcr). multiple-step protocols using several additives to inhibit polyphenolic compounds during nucleic acid extraction are common, but time consuming and laborious. the current research highlights that the inclusion of 0.65 to 0.70% of sodium sulphite in the extraction buffer minimizes the pigmentation of nucleic acid extracts and improves the rt-pcr detection of potato virus y (pvy) and potato leaf ... | 2002 | 11684310 |
| evidence for rna-mediated defence effects on the accumulation of potato leafroll virus. | in plants infected with potato leafroll virus (plrv), or other luteoviruses, infection is very largely confined to cells in the vascular system. even in tobacco plants transformed with plrv full-length cdna, in which all mesophyll cells should synthesize infectious plrv rna transcripts, only a minority of the mesophyll cells accumulate detectable amounts of virus. we have explored this phenomenon further by transforming a better plrv host, nicotiana benthamiana, with the same transgene, by super ... | 2001 | 11714988 |
| a major quantitative trait locus for resistance to potato leafroll virus is located in a resistance hotspot on potato chromosome xi and is tightly linked to n-gene-like markers. | potato leafroll virus (plrv) causes one of the most widespread and important virus diseases in potato. resistance to plrv is controlled by genetic factors that limit plant infection by viruliferous aphids or virus multiplication and accumulation. quantitative trait locus (qtl) analysis of resistance to virus accumulation revealed one major and two minor qtl. the major qtl, plrv.1, mapped to potato chromosome xi in a resistance hotspot containing several genes for qualitative and quantitative res ... | 2001 | 11768537 |