| the effects of bean leafroll virus on life history traits and host selection behavior of specialized pea aphid (acyrthosiphon pisum, hemiptera: aphididae) genotypes. | intraspecific specialization by insect herbivores on different host plant species contributes to the formation of genetically distinct "host races," but the effects of plant virus infection on interactions between specialized herbivores and their host plants have barely been investigated. using three genetically and phenotypically divergent pea aphid clones (acyrthosiphon pisum l.) adapted to either pea (pisum sativum l.) or alfalfa (medicago sativa l.), we tested how infection of these hosts by ... | 2017 | 28062535 |
| detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia. | a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ... | 2017 | 28855829 |
| detection and discrimination of members of the family luteoviridae by real-time pcr and sybr® greener™ melting curve analysis. | this study investigated the suitability of a two step real-time rt-pcr melting curve analysis as a tool for the detection and discrimination of nine species in the plant virus family luteoviridae, being soybean dwarf virus [sbdv], bean leafroll virus [blrv], beet chlorosis virus [bchv], beet mild yellowing virus [bmyv], beet western yellows virus [bwyv], cereal yellow dwarf virus-rpv [cydv-rpv], cucurbit aphid-borne yellows virus [cabyv], potato leafroll virus [plrv] and turnip yellows virus [tu ... | 2010 | 20933015 |
| molecular characterization of pea enation mosaic virus and bean leafroll virus from the pacific northwest, usa. | the family luteoviridae consists of eight viruses assigned to three different genera, luteovirus, polerovirus and enamovirus. the complete genomic sequences of pea enation mosaic virus (genus enamovirus) and bean leafroll virus (genus luteovirus) from the pacific northwest, usa, were determined. annotation, sequence comparisons, and phylogenetic analysis of selected genes together with those of known polero- and enamoviruses were conducted. | 2010 | 20697921 |
| epidemiology and integrated management of persistently transmitted aphid-borne viruses of legume and cereal crops in west asia and north africa. | cool-season food legumes (faba bean, lentil, chickpea and pea) and cereals (bread and durum wheat and barley) are the most important and widely cultivated crops in west asia and north africa (wana), where they are the main source of carbohydrates and protein for the majority of the population. persistently transmitted aphid-borne viruses pose a significant limitation to legume and cereal production worldwide. surveys conducted in many countries in wana during the last three decades established t ... | 2009 | 19152820 |
| nucleotide sequence shows that bean leafroll virus has a luteovirus-like genome organization. | the complete nucleotide sequence of the bean leafroll virus (blrv) genomic rna and the termini of its smallest subgenomic rnas were determined to better understand its mechanisms of gene expression and replication and its phylogenetic position within the luteoviridae: the number and placement of open reading frames (orfs) within the blrv genome was luteovirus-like. the nucleotide and predicted amino acid sequences of blrv were most similar to those of soybean dwarf virus (sbdv). phylogenetic ana ... | 2002 | 12075101 |
| nucleotide sequence and e. coli expression of the coat protein gene of the yellowing strain of soybean dwarf luteovirus. | the nucleotide sequence of the coat protein gene of the yellowing (y) strain of soybean dwarf virus (sbdv) was determined from cloned cdna. the gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated m(r) of 22,200. the identity of the open reading frame (orf) encoding the coat protein was confirmed by expression of an escherichia coli beta-galactosidase fusion protein and detection by a dot blot immunoassay. sequence comparisons of the deduced coat protein amino ... | 1993 | 8240014 |
| relationships among luteoviruses based on nucleic acid hybridization and serological studies. | the luteoviruses barley yellow dwarf virus (bydv-pav and bydv-rpv), bean leaf roll virus (blrv) beet western yellows virus (bwyv), carrot red leaf virus, potato leaf roll virus, and soybean dwarf virus (sdv) were compared by hybridization with random cdna probes and serologically with polyclonal antisera. for hybridizations, filters had each rna blotted in duplicate dots at 10 ng/dot. random-primed cdna probes were prepared from 300 ng of each rna and used to probe filters at three levels of str ... | 1990 | 2338398 |
| a characterization of epitopes on potato leafroll virus coat protein. | a panel of ten stable hybridoma cell lines secreting monoclonal antibodies (mabs) specific for potato leafroll virus (plrv) antigen, was produced in two fusion experiments with murine splenic and myeloma cells. using different elisa procedures and western blotting it was shown that one mab detected a continuous epitope and nine mabs reacted with conformation-dependent ones. the conformation-dependent epitopes could be separated into two groups after alkaline treatment of the virions. the mabs we ... | 1990 | 1701986 |